Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0005739

Physical Location & Seq

Genomic Coordinates Forward (+)

22648639 .. 22655851

Gene Structure 7213 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1138300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2769 bp

ATGATGGAAAACGGAGCTAGTTGCTTACCCAACACGCCGGAGGAGGAGAAGCGGATCGTCGATGAATTGGCCCGAAAATCGGAGGCCAATGTCAAGGAAGGCAACTTGTTTTATGCTGTTTCCAATGGGTGGTACTCAAGCTGGAAGAGGTATGTTGAGCAAGGTGCTGGAGAACATCTATATGATGAGCAGGATTCTGTGTCTCATAATTTGGATATTTTCTCATCTAAAATTATAGCTAGGCCTGGACCTATTGATAATTCTGATATAGTTGTAAGTGAATCTGAGGGAAACGATTTACAGCTTCATAGAACACTGCTGGAAGACCTTGACTATGTCTTAGTTTCTCAAGAAGTTTGGGAAAAGCTGTTCGACTGGTACAAAGGAGGCCCAGCATTACCAAGAAAGATGATTTCTCAGGGTGATCTTATCAAGAATCTGATGGTGGAGGTCTACCCATTATGTCTGAAGATTATCGATTCAAGAGACAACAGCCAGACAGTTATAAGGTTAAGCAAAAAGGCTTCTGTACAGGAGCTTTACGAGAAGGTGTGCAGAATTAGAGGAATAGAGCAACAAAAGGCTCGTATTTGGGACTACTTCAACATGGAGAAGAATTCATTGTTGGTGGTTTCAAACCAAACCCTAGAGCAGCTGAACTTGCAGATGGATCAAGAAATTCTTCTTGAGGTGCAAACTGATGCAAACTTTTCTTCTCAATTCTCCATGGATTCAACAGGAAATGAGTTAGCATTGGTACCCCTGGAACCCTCAAGGTCATCTATCACAATTGCAGGGGGTCCTTCCTTGTCAAATGGTCATTCAATGGATTATAAAAGTTTTAATTTGCACCAGGGAAGTGCCTTAAGCAGCTCAACGTCAACAGATACAGATGATAAATATTATGCTTATAATCCAATGAAAAAAGTAGATAGGGGAGGTTTGGCTGGATTGCAAAATCTTGGAAACACTTGTTTTATGAATAGTTCACTTCAGTGCTTAGTCCACACACCTCCGCTTGTTGAGTATTTCTTGCAAGATTACTCTGATGAGATCAACACTGACAATCCCTTAGGAATGCGTGGTGAGCTTGCTCTTGCCTTCGGTGAGTTGTTGAGAAAATTGTGGTCATCAGGACGAACGACAATTGCACCACGTGCTTTCAAGGGAAAACTTTCTCGATTTGCTCCCCAGTTTAGTGGCTATAATCAACATGATTCTCAAGAACTTCTTGCTTTCTTACTTGATGGACTACATGAAGATTTGAATCGTGTCAAGAATAAGCCATACATTGAAACAAAAGATTCAGATGGTCGTCCAGATGACGAAGTTGCAATTGAGTGTTGGAAAAATCACAGAGCCCGAAATGATTCATTGATTGTTGATGTTTGCCAAGGTCAATATAAATCAACATTGGTATGTCCAGTTTGTAGCAAGGTTTCAATTACATTTGATCCCTTTATGTACTTATCATTGCCACTACCTTCAACTGTCACCCGGCCAATGACGGTGACTGTGCTTTATGGTGATGGGCGTGGTCTTCCAAGGCCATACACTGTAACCATGATAAAAGATCAATGCTGTAAGAATCTTATTGAGGCATTGGCTACCGCATGCTGCTTGAGGAGTGATGAAACCCTTCTGCTTGCTGAGGTATATGAACATCGAATTTATCGATATCTGGACAACCTTTCTGAACCATTGTCTTCAATTAAGAATGATGATCGGATTGTGGCCTATCGGTATTCTAAAGAAAAAGTAGCTTCCAGTACCAGACTTGAAATAATTCATCGACGGCAGGAAAAATGTGCATTAGATCCTCTGAAGGGTCAGAGAAAGCTTTTTGGAACACCTTTGGTTGCCTATCTAGGAGAAGACCAAATACGTGGTGTTGATGTTGATAGAGCAGTTTCTAGAATTCTGTCACCTTTGAAGAGAGTAGTTAAGCTTCATAGCACGAAAGAAAATGGATTAGTTTCAGAAGCCATTGCTGAACCATCAAACAACCACTTTTTGAGGCCCACGGAGAACATAGAACTAGGGGAAACATCAATTGGTGAGTTATCCTTCCAGCTATTTTTAGCTGATGAGAGGGGTATGACCTGCAAACCAATTGAAAAGTCTTCGTCCCTAAGTTCTGGTAAACTCGTAAAGATATTTGTGGACTGGACTGACCAAGAAAACGAAGTATATGATGCTAGCTATCTCATGGATCTACCTGAGGTATACAAGAATGTGTTCACAGCGAAGAAAACTAGGCAAGAAGCTATTTCCTTATTTACATGCTTGGAGGCATTTTTGAAGGAAGAACCTCTAGGTCCCGATGATATGTGGTACTGCCCTAAGTGCAAGGAACATAGACAAGCTACAAAGAAACTAGATTTGTGGATGTTGCCAGAGGTTCTTGTTTTTCACCTGAAACGGTTCTCATACAGCAGATACTCGAAGAATAAACTTGATACTCTGGTGACTTTTCCAATTCATAATCTTAATTTGAGTCAATATGTGAAAAGCAAGGATGGAAAAGCTCATCTGTATGAGCTATACGCCATTAGCAACCATTACGGCGGTCTAGGTGGTGGGCACTATACTGCATATGCAAAGTTGATTGATGAGAACAGATGGTACCATTTTGATGACAGTCATGTATCTCCAGTCAATGAAACTGATATCAAGACTTCGGCCGCCTATGTGTTATTTTATCGGAGAGTTAAAAGTGGACAGAACACCGGAGAGGCGGAGTCATCCGGGACTCATATGGAATCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04137 map04137

Pfam Domains

Protein Families

DUSP UCH

Protein Analysis

923

Amino Acids

104.59

Weight (kDa)

5.71

Isoelectric Point (pI)

40.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUSP	PF06337	35 - 138	5.5e-22	DUSP domain
UCH	PF00443	316 - 901	6e-77	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	316 - 495	1.2e-06	Ubiquitin carboxyl-terminal hydrolase
UCH_1	PF13423	728 - 883	5.2e-06	Ubiquitin carboxyl-terminal hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005739)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G32850 AT4G10570 AT4G10590 AT4G10590 AT4G10590 AT4G10590
fragaria_vesca	FvH4_5g10610 FvH4_5g10610 FvH4_5g10610
malus_domestica	MD06G1121900.v1.1 MD14G1138300.v1.1
prunus_persica	Prupe.5G132600_v2.0.a1 Prupe.5G132600_v2.0.a1 Prupe.5G132600_v2.0.a1
pyrus_communis	pycom06g11300 pycom14g11340
rosa_chinensis	RchiOBHm_Chr7g0187531
rosa_laevigata	RLG00000004773
rosa_multiflora	Rmu_sc0008640.1_g000004 Rmu_sc0014966.1_g000001 Rmu_sc0023622.1_g000001 Rmu_ssc0000110.1_g000024
rosa_roxburghii	Rroxscaffold_3G00267350
rosa_rugosa	Rorug06G0482500
rosa_samantha	Rh7AG087100 Rh7BG088500 Rh7CG087800 Rh7DG089400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	506, 834, 912
Acc36I	ACCTGC	1 cut(s)	2111
Acc65I	GGTACC	2 cut(s)	757, 2625
AccB1I	GGYRCC	2 cut(s)	757, 2625
AccB7I	CCANNNNNTGG	1 cut(s)	129
AccI	GTMKAC	2 cut(s)	453, 2226
AciI	CCGC	6 cut(s)	52, 1016, 1611, 2568, 2685, 2738
AclWI	GGATC	5 cut(s)	62, 678, 1448, 1811, 2220
AcoI	YGGCCR	2 cut(s)	1499, 2682
AcsI	RAATTY	4 cut(s)	616, 678, 1668, 1917
AcuI	CTGAAG	3 cut(s)	488, 977, 1844
AcvI	CACGTG	1 cut(s)	1157
AdeI	CACNNNGTG	1 cut(s)	1157
AfaI	GTAC	8 cut(s)	134, 380, 531, 759, 1466, 1771, 2336, 2627
AfiI	CCNNNNNNNGG	3 cut(s)	79, 129, 1826
AflII	CTTAAG	1 cut(s)	865
AjnI	CCWGG	3 cut(s)	244, 762, 852
AjuI	GAANNNNNNNTTGG	4 cut(s)	608, 640, 2037, 2069
AleI	CACNNNNGTG	1 cut(s)	994
AloI	GAACNNNNNNTCC	2 cut(s)	353, 385
Alw26I	GTCTC	2 cut(s)	207, 480
AlwI	GGATC	5 cut(s)	62, 678, 1448, 1811, 2220
AoxI	GGCC	9 cut(s)	69, 84, 242, 388, 1499, 1547, 1734, 2018, 2682
ApeKI	GCWGC	3 cut(s)	652, 870, 1617
ApoI	RAATTY	4 cut(s)	616, 678, 1668, 1917
Asp700I	GAANNNNTTC	7 cut(s)	681, 1174, 1369, 1638, 1785, 2237, 2423
Asp718I	GGTACC	2 cut(s)	757, 2625
AspS9I	GGNCC	6 cut(s)	70, 248, 389, 800, 2019, 2318
AsuC2I	CCSGG	2 cut(s)	1498, 2749
AsuNHI	GCTAGC	1 cut(s)	2198
AvaII	GGWCC	3 cut(s)	248, 800, 2318
AxyI	CCTNAGG	2 cut(s)	1072, 2220
BaeGI	GKGCMC	1 cut(s)	2586
BaeI	ACNNNNGTAYC	2 cut(s)	2451, 2484
BanI	GGYRCC	2 cut(s)	757, 2625
BanII	GRGCYC	1 cut(s)	1363
BarI	GAAGNNNNNNTAC	4 cut(s)	1866, 1898, 1932, 1964
BbrPI	CACGTG	1 cut(s)	1157
BbsI	GAAGAC	5 cut(s)	330, 1532, 1698, 1881, 2115
BbvCI	CCTCAGC	1 cut(s)	1650
BbvI	GCAGC	3 cut(s)	664, 882, 1604
BccI	CCATC	8 cut(s)	436, 661, 1241, 1304, 1523, 2005, 2513, 2616
BceAI	ACGGC	2 cut(s)	1811, 2581
BciT130I	CCWGG	3 cut(s)	246, 764, 854
BcnI	CCSGG	2 cut(s)	1498, 2749
BcoDI	GTCTC	2 cut(s)	207, 480
BfrI	CTTAAG	1 cut(s)	865
BfuAI	ACCTGC	1 cut(s)	2111
BisI	GCNGC	4 cut(s)	653, 871, 1618, 2685
BlsI	GCNGC	4 cut(s)	654, 872, 1619, 2686
Bme1390I	CCNGG	5 cut(s)	246, 764, 854, 1498, 2749
Bme18I	GGWCC	3 cut(s)	248, 800, 2318
BmgT120I	GGNCC	6 cut(s)	70, 248, 389, 800, 2019, 2318
BmiI	GGNNCC	5 cut(s)	759, 768, 801, 2320, 2627
BmrFI	CCNGG	5 cut(s)	246, 764, 854, 1498, 2749
BmrI	ACTGGG	1 cut(s)	1186
BmsI	GCATC	2 cut(s)	691, 2185
BmtI	GCTAGC	1 cut(s)	2202
BmuI	ACTGGG	1 cut(s)	1186
BpiI	GAAGAC	5 cut(s)	330, 1532, 1698, 1881, 2115
BpmI	CTGGAG	2 cut(s)	189, 2637
Bpu10I	CCTNAGC	1 cut(s)	1650
BpuEI	CTTGAG	6 cut(s)	121, 333, 707, 757, 1206, 1642
BpuMI	CCSGG	2 cut(s)	1498, 2749
Bsa29I	ATCGAT	2 cut(s)	477, 1675
BsaAI	YACGTR	2 cut(s)	1157, 1886
BsaBI	GATNNNNATC	1 cut(s)	1266
BsaJI	CCNNGG	6 cut(s)	726, 762, 853, 1393, 1544, 2022
BsaWI	WCCGGW	1 cut(s)	2729
Bsc4I	CCNNNNNNNGG	3 cut(s)	79, 129, 1826
Bse1I	ACTGG	6 cut(s)	380, 1192, 1424, 1767, 2171, 2654
Bse21I	CCTNAGG	2 cut(s)	1072, 2220
Bse3DI	GCAATG	2 cut(s)	1472, 1986
Bse8I	GATNNNNATC	1 cut(s)	1266
BseBI	CCWGG	3 cut(s)	246, 764, 854
BseCI	ATCGAT	2 cut(s)	477, 1675
BseDI	CCNNGG	6 cut(s)	726, 762, 853, 1393, 1544, 2022
BseGI	GGATG	3 cut(s)	2394, 2524, 2744
BseJI	GATNNNNATC	1 cut(s)	1266
BseLI	CCNNNNNNNGG	3 cut(s)	79, 129, 1826
BseMI	GCAATG	2 cut(s)	1472, 1986
BseMII	CTCAG	4 cut(s)	276, 431, 1641, 2211
BseNI	ACTGG	6 cut(s)	380, 1192, 1424, 1767, 2171, 2654
BseRI	GAGGAG	3 cut(s)	56, 59, 1639
BseSI	GKGCMC	1 cut(s)	2586
BseX3I	CGGCCG	1 cut(s)	2682
BseXI	GCAGC	3 cut(s)	664, 882, 1604
BseYI	CCCAGC	1 cut(s)	391
BsgI	GTGCAG	1 cut(s)	574
Bsh1285I	CGRYCG	1 cut(s)	2685
BshFI	GGCC	9 cut(s)	71, 86, 244, 390, 1501, 1549, 1736, 2020, 2684
BshNI	GGYRCC	2 cut(s)	757, 2625
BshVI	ATCGAT	2 cut(s)	477, 1675
BsiEI	CGRYCG	1 cut(s)	2685
BsiSI	CCGG	4 cut(s)	38, 1498, 2730, 2748
BslFI	GGGAC	4 cut(s)	608, 2113, 2304, 2764
BslI	CCNNNNNNNGG	3 cut(s)	79, 129, 1826
BsmAI	GTCTC	2 cut(s)	207, 480
BsmFI	GGGAC	4 cut(s)	608, 2113, 2304, 2764
BsmI	GAATGC	1 cut(s)	1083
BsnI	GGCC	9 cut(s)	71, 86, 244, 390, 1501, 1549, 1736, 2020, 2684
Bsp1286I	GDGCHC	2 cut(s)	1363, 2586
Bsp1407I	TGTACA	1 cut(s)	529
Bsp143I	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
Bsp19I	CCATGG	1 cut(s)	726
BspACI	CCGC	6 cut(s)	52, 1016, 1611, 2568, 2685, 2738
BspANI	GGCC	9 cut(s)	71, 86, 244, 390, 1501, 1549, 1736, 2020, 2684
BspCNI	CTCAG	4 cut(s)	277, 430, 1642, 2212
BspDI	ATCGAT	2 cut(s)	477, 1675
BspLI	GGNNCC	5 cut(s)	759, 768, 801, 2320, 2627
BspMI	ACCTGC	1 cut(s)	2111
BspOI	GCTAGC	1 cut(s)	2202
BspPI	GGATC	5 cut(s)	62, 678, 1448, 1811, 2220
BspT107I	GGYRCC	2 cut(s)	757, 2625
BspTI	CTTAAG	1 cut(s)	865
BsrDI	GCAATG	2 cut(s)	1472, 1986
BsrGI	TGTACA	1 cut(s)	529
BsrI	ACTGG	6 cut(s)	380, 1192, 1424, 1767, 2171, 2654
BssECI	CCNNGG	6 cut(s)	726, 762, 853, 1393, 1544, 2022
BssMI	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
BssNAI	GTATAC	1 cut(s)	2227
BssT1I	CCWWGG	3 cut(s)	726, 1393, 1544
Bst1107I	GTATAC	1 cut(s)	2227
Bst2UI	CCWGG	3 cut(s)	246, 764, 854
Bst4CI	ACNGT	7 cut(s)	502, 1492, 1510, 1516, 1558, 2424, 2642
Bst6I	CTCTTC	2 cut(s)	140, 1928
BstAFI	CTTAAG	1 cut(s)	865
BstAPI	GCANNNNNTGC	1 cut(s)	1157
BstAUI	TGTACA	1 cut(s)	529
BstBAI	YACGTR	2 cut(s)	1157, 1886
BstC8I	GCNNGC	4 cut(s)	1092, 1615, 1647, 2200
BstDEI	CTNAG	9 cut(s)	285, 340, 417, 1000, 1072, 1650, 2132, 2220, 2343
BstDSI	CCRYGG	2 cut(s)	726, 2022
BstF5I	GGATG	3 cut(s)	2394, 2524, 2744
BstKTI	GATC	9 cut(s)	57, 427, 673, 1056, 1456, 1576, 1726, 1819, 2215
BstMAI	GTCTC	2 cut(s)	207, 480
BstMBI	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
BstMCI	CGRYCG	1 cut(s)	2685
BstMWI	GCNNNNNNNGC	4 cut(s)	661, 1087, 1157, 2346
BstNI	CCWGG	3 cut(s)	246, 764, 854
BstNSI	RCATGY	2 cut(s)	1617, 2286
BstSCI	CCNGG	5 cut(s)	244, 762, 852, 1496, 2747
BstSLI	GKGCMC	1 cut(s)	2586
BstV1I	GCAGC	3 cut(s)	664, 882, 1604
BstV2I	GAAGAC	5 cut(s)	330, 1532, 1698, 1881, 2115
BstX2I	RGATCY	2 cut(s)	1816, 2212
BstXI	CCANNNNNNTGG	2 cut(s)	1199, 1886
BstYI	RGATCY	2 cut(s)	1816, 2212
BstZ17I	GTATAC	1 cut(s)	2227
BstZI	CGGCCG	1 cut(s)	2682
Bsu15I	ATCGAT	2 cut(s)	477, 1675
Bsu36I	CCTNAGG	2 cut(s)	1072, 2220
BsuRI	GGCC	9 cut(s)	71, 86, 244, 390, 1501, 1549, 1736, 2020, 2684
BsuTUI	ATCGAT	2 cut(s)	477, 1675
BtgI	CCRYGG	2 cut(s)	726, 2022
BtsCI	GGATG	3 cut(s)	2394, 2524, 2744
BtsI	GCAGTG	1 cut(s)	314
BtsIMutI	CAGTG	4 cut(s)	314, 1001, 1059, 1554
BveI	ACCTGC	1 cut(s)	2111
Cac8I	GCNNGC	4 cut(s)	1092, 1615, 1647, 2200
Cfr13I	GGNCC	6 cut(s)	70, 248, 389, 800, 2019, 2318
ClaI	ATCGAT	2 cut(s)	477, 1675
Csp6I	GTAC	8 cut(s)	133, 379, 530, 758, 1465, 1770, 2335, 2626
CviAII	CATG	9 cut(s)	607, 727, 1214, 1256, 1564, 1614, 2209, 2283, 2645
CviQI	GTAC	8 cut(s)	133, 379, 530, 758, 1465, 1770, 2335, 2626
DdeI	CTNAG	9 cut(s)	285, 340, 417, 1000, 1072, 1650, 2132, 2220, 2343
DpnI	GATC	9 cut(s)	56, 426, 672, 1055, 1455, 1575, 1725, 1818, 2214
DpnII	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
DraIII	CACNNNGTG	1 cut(s)	1157
EaeI	YGGCCR	2 cut(s)	1499, 2682
EagI	CGGCCG	1 cut(s)	2682
Eam1104I	CTCTTC	2 cut(s)	140, 1928
EarI	CTCTTC	2 cut(s)	140, 1928
EciI	GGCGGA	1 cut(s)	2753
EclXI	CGGCCG	1 cut(s)	2682
Eco130I	CCWWGG	3 cut(s)	726, 1393, 1544
Eco147I	AGGCCT	1 cut(s)	244
Eco24I	GRGCYC	1 cut(s)	1363
Eco32I	GATATC	2 cut(s)	1679, 2671
Eco47I	GGWCC	3 cut(s)	248, 800, 2318
Eco52I	CGGCCG	1 cut(s)	2682
Eco57I	CTGAAG	3 cut(s)	488, 977, 1844
Eco72I	CACGTG	1 cut(s)	1157
Eco81I	CCTNAGG	2 cut(s)	1072, 2220
EcoO109I	RGGNCCY	2 cut(s)	800, 2318
EcoRI	GAATTC	2 cut(s)	616, 1917
EcoRII	CCWGG	3 cut(s)	244, 762, 852
EcoRV	GATATC	2 cut(s)	1679, 2671
EcoT14I	CCWWGG	3 cut(s)	726, 1393, 1544
EcoT38I	GRGCYC	1 cut(s)	1363
ErhI	CCWWGG	3 cut(s)	726, 1393, 1544
FaeI	CATG	9 cut(s)	610, 730, 1217, 1259, 1567, 1617, 2212, 2286, 2648
FalI	AAGNNNNNCTT	4 cut(s)	666, 698, 1215, 1247
FaqI	GGGAC	4 cut(s)	608, 2113, 2304, 2764
FatI	CATG	9 cut(s)	606, 726, 1213, 1255, 1563, 1613, 2208, 2282, 2644
FauNDI	CATATG	2 cut(s)	2596, 2757
FblI	GTMKAC	2 cut(s)	453, 2226
Fnu4HI	GCNGC	4 cut(s)	653, 871, 1618, 2685
FokI	GGATG	3 cut(s)	2401, 2531, 2731
FriOI	GRGCYC	1 cut(s)	1363
Fsp4HI	GCNGC	4 cut(s)	653, 871, 1618, 2685
GluI	GCNGC	4 cut(s)	653, 871, 1618, 2685
GsaI	CCCAGC	1 cut(s)	395
GsuI	CTGGAG	2 cut(s)	189, 2637
HaeIII	GGCC	9 cut(s)	71, 86, 244, 390, 1501, 1549, 1736, 2020, 2684
HapII	CCGG	4 cut(s)	38, 1498, 2730, 2748
Hin1II	CATG	9 cut(s)	610, 730, 1217, 1259, 1567, 1617, 2212, 2286, 2648
HincII	GTYRAC	1 cut(s)	882
HindII	GTYRAC	1 cut(s)	882
HindIII	AAGCTT	2 cut(s)	1838, 1946
HpaII	CCGG	4 cut(s)	38, 1498, 2730, 2748
Hpy166II	GTNNAC	9 cut(s)	454, 882, 989, 1006, 2144, 2164, 2227, 2241, 2720
Hpy8I	GTNNAC	9 cut(s)	454, 882, 989, 1006, 2144, 2164, 2227, 2241, 2720
Hpy99I	CGWCG	2 cut(s)	62, 1797
HpyAV	CCTTC	9 cut(s)	92, 541, 813, 1111, 1494, 1649, 1819, 2077, 2296
HpyCH4III	ACNGT	7 cut(s)	502, 1492, 1510, 1516, 1558, 2424, 2642
HpyCH4IV	ACGT	3 cut(s)	878, 1156, 1885
HpyF10VI	GCNNNNNNNGC	4 cut(s)	661, 1087, 1157, 2346
HpyF3I	CTNAG	9 cut(s)	285, 340, 417, 1000, 1072, 1650, 2132, 2220, 2343
HpySE526I	ACGT	3 cut(s)	878, 1156, 1885
Hsp92II	CATG	9 cut(s)	610, 730, 1217, 1259, 1567, 1617, 2212, 2286, 2648
KpnI	GGTACC	2 cut(s)	761, 2629
Kzo9I	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
LmnI	GCTCC	3 cut(s)	14, 535, 1192
Lsp1109I	GCAGC	3 cut(s)	664, 882, 1604
LweI	GCATC	2 cut(s)	691, 2185
MaeII	ACGT	3 cut(s)	878, 1156, 1885
MaeIII	GTNAC	5 cut(s)	1492, 1510, 1558, 1923, 2467
MalI	GATC	9 cut(s)	56, 426, 672, 1055, 1455, 1575, 1725, 1818, 2214
MboI	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
MfeI	CAATTG	5 cut(s)	789, 1146, 1335, 2052, 2112
MflI	RGATCY	2 cut(s)	1816, 2212
MhlI	GDGCHC	2 cut(s)	1363, 2586
MlyI	GAGTC	3 cut(s)	2506, 2746, 2750
MmeI	TCCRAC	1 cut(s)	1325
MroXI	GAANNNNTTC	7 cut(s)	681, 1174, 1369, 1638, 1785, 2237, 2423
MseI	TTAA	7 cut(s)	512, 843, 866, 1713, 1944, 2490, 2712
MslI	CAYNNNNRTG	5 cut(s)	180, 994, 1417, 2535, 2634
MspA1I	CMGCKG	1 cut(s)	655
MspCI	CTTAAG	1 cut(s)	865
MspI	CCGG	4 cut(s)	38, 1498, 2730, 2748
MspR9I	CCNGG	5 cut(s)	246, 764, 854, 1498, 2749
MunI	CAATTG	5 cut(s)	789, 1146, 1335, 2052, 2112
Mva1269I	GAATGC	1 cut(s)	1083
MvaI	CCWGG	3 cut(s)	246, 764, 854
MwoI	GCNNNNNNNGC	4 cut(s)	661, 1087, 1157, 2346
NciI	CCSGG	2 cut(s)	1498, 2749
NcoI	CCATGG	1 cut(s)	726
NdeI	CATATG	2 cut(s)	2596, 2757
NdeII	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
NheI	GCTAGC	1 cut(s)	2198
NlaIII	CATG	9 cut(s)	610, 730, 1217, 1259, 1567, 1617, 2212, 2286, 2648
NlaIV	GGNNCC	5 cut(s)	759, 768, 801, 2320, 2627
NmuCI	GTSAC	4 cut(s)	1492, 1510, 1923, 2467
NspI	RCATGY	2 cut(s)	1617, 2286
OliI	CACNNNNGTG	1 cut(s)	994
PaeI	GCATGC	1 cut(s)	1617
PceI	AGGCCT	1 cut(s)	244
PcsI	WCGNNNNNNNCGW	1 cut(s)	1672
PctI	GAATGC	1 cut(s)	1083
PdmI	GAANNNNTTC	7 cut(s)	681, 1174, 1369, 1638, 1785, 2237, 2423
PflFI	GACNNNGTC	1 cut(s)	335
PflMI	CCANNNNNTGG	1 cut(s)	129
PfoI	TCCNGGA	1 cut(s)	2747
PkrI	GCNGC	4 cut(s)	654, 872, 1619, 2686
PleI	GAGTC	3 cut(s)	2505, 2746, 2749
PmaCI	CACGTG	1 cut(s)	1157
PmlI	CACGTG	1 cut(s)	1157
PpsI	GAGTC	3 cut(s)	2505, 2746, 2749
Ppu21I	YACGTR	2 cut(s)	1157, 1886
PpuMI	RGGWCCY	2 cut(s)	800, 2318
PsiI	TTATAA	3 cut(s)	506, 834, 912
Psp5II	RGGWCCY	2 cut(s)	800, 2318
Psp6I	CCWGG	3 cut(s)	244, 762, 852
PspCI	CACGTG	1 cut(s)	1157
PspFI	CCCAGC	1 cut(s)	391
PspGI	CCWGG	3 cut(s)	244, 762, 852
PspN4I	GGNNCC	5 cut(s)	759, 768, 801, 2320, 2627
PspPI	GGNCC	6 cut(s)	70, 248, 389, 800, 2019, 2318
PspPPI	RGGWCCY	2 cut(s)	800, 2318
PsrI	GAACNNNNNNTAC	2 cut(s)	2609, 2641
PsuI	RGATCY	2 cut(s)	1816, 2212
PsyI	GACNNNGTC	1 cut(s)	335
PvuII	CAGCTG	1 cut(s)	655
RsaI	GTAC	8 cut(s)	134, 380, 531, 759, 1466, 1771, 2336, 2627
RsaNI	GTAC	8 cut(s)	133, 379, 530, 758, 1465, 1770, 2335, 2626
RseI	CAYNNNNRTG	5 cut(s)	180, 994, 1417, 2535, 2634
SaqAI	TTAA	7 cut(s)	512, 843, 866, 1713, 1944, 2490, 2712
SatI	GCNGC	4 cut(s)	653, 871, 1618, 2685
Sau3AI	GATC	9 cut(s)	54, 424, 670, 1053, 1453, 1573, 1723, 1816, 2212
Sau96I	GGNCC	6 cut(s)	70, 248, 389, 800, 2019, 2318
SchI	GAGTC	3 cut(s)	2506, 2746, 2750
ScrFI	CCNGG	5 cut(s)	246, 764, 854, 1498, 2749
SduI	GDGCHC	2 cut(s)	1363, 2586
SfaNI	GCATC	2 cut(s)	691, 2185
SinI	GGWCC	3 cut(s)	248, 800, 2318
SmiMI	CAYNNNNRTG	5 cut(s)	180, 994, 1417, 2535, 2634
SmlI	CTYRAG	7 cut(s)	136, 348, 686, 772, 865, 1221, 1621
SmoI	CTYRAG	7 cut(s)	136, 348, 686, 772, 865, 1221, 1621
SphI	GCATGC	1 cut(s)	1617
SseBI	AGGCCT	1 cut(s)	244
SsiI	CCGC	6 cut(s)	52, 1016, 1611, 2568, 2685, 2738
SspI	AATATT	1 cut(s)	902
StuI	AGGCCT	1 cut(s)	244
StyD4I	CCNGG	5 cut(s)	244, 762, 852, 1496, 2747
StyI	CCWWGG	3 cut(s)	726, 1393, 1544
TaaI	ACNGT	7 cut(s)	502, 1492, 1510, 1516, 1558, 2424, 2642
TaiI	ACGT	3 cut(s)	881, 1159, 1888
TaqI	TCGA	8 cut(s)	60, 372, 477, 1180, 1666, 1675, 1792, 2444
TatI	WGTACW	2 cut(s)	529, 1464
TauI	GCSGC	1 cut(s)	2687
Tru1I	TTAA	7 cut(s)	512, 843, 866, 1713, 1944, 2490, 2712
Tru9I	TTAA	7 cut(s)	512, 843, 866, 1713, 1944, 2490, 2712
TscAI	CASTG	4 cut(s)	321, 1001, 1066, 1561
TseFI	GTSAC	4 cut(s)	1492, 1510, 1923, 2467
TseI	GCWGC	3 cut(s)	652, 870, 1617
Tsp45I	GTSAC	4 cut(s)	1492, 1510, 1923, 2467
TspGWI	ACGGA	2 cut(s)	27, 2039
TspRI	CASTG	4 cut(s)	321, 1001, 1066, 1561
Tth111I	GACNNNGTC	1 cut(s)	335
Van91I	CCANNNNNTGG	1 cut(s)	129
Vha464I	CTTAAG	1 cut(s)	865
VpaK11BI	GGWCC	3 cut(s)	248, 800, 2318
XapI	RAATTY	4 cut(s)	616, 678, 1668, 1917
XbaI	TCTAGA	1 cut(s)	1913
XceI	RCATGY	2 cut(s)	1617, 2286
XmiI	GTMKAC	2 cut(s)	453, 2226
XmnI	GAANNNNTTC	7 cut(s)	681, 1174, 1369, 1638, 1785, 2237, 2423

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.