MD06G1135700.v1.1
NAC Family

NAC transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0007675
Physical Location & Seq
Reverse (-)
28061855 .. 28063088
1234 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1135700.v1.1.491

Sequence Viewer

Length: 681 bp
ATGGACAAGTTCAAATTTGTTAGAAATGGGATGATCAGATTGCCTCCTGGTTTCCGATTCCAACCAACAGATGAAGAGCTAGTTTTTCAGTACCTGAGATGCAAAGTCTTCTCATGCCCACTTCCTGCTTCCATTATTCCCGAGATCAACGTTTGCATGTATGATCCTTGGGATTTGCCAGGTAATTTGGAACAAGAGAGGTATTTCTTCAGCAACAAGGAGTCAAAATACCGGAATGGAAACCGAGCCAACAGGGTGACAAGTTCCGGTTACTGGAAAGCAACCGGCGCAGATAAAAAGATTGTATCTTCAAGGAGGAATCATATTGTGGGAAAGAAAAAGACTCTAGTATTTTACAGAGGGAAGTCTCCACATGTTTCTAAGACTGATTGGGTCATGCATGAATATTGCCTTGTGAATGCAGAAACTACAGCTTCCATTCACGCAACTGAGAATGCTTTAACGCCAAAAGGAAATTGGGTTTTGTGTCGGGTTTTTTCTAAGAAAAGAAGTGGCAAGATGAATGAGGAGATTGTGGTGAATTACAACAGCATCAAAGTTAACAATAATGCGAATCCTGCATCTTCTTCCTCCTCTTGTTCAAGTTCAAGTGGGATCACAGAAGTAACTTCCCCAAGTGAAGAATGTGGTGAAGAAATCAGTAGCTGCCCTAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

227

Amino Acids

25.56

Weight (kDa)

9.23

Isoelectric Point (pI)

58.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 15 - 138 4.3e-44 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 150
AclWI GGATC 2 cut(s) 158, 623
AcsI RAATTY 2 cut(s) 14, 674
AcuI CTGAAG 1 cut(s) 193
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 1 cut(s) 273
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 4 cut(s) 13, 312, 603, 609
AjnI CCWGG 2 cut(s) 46, 178
AluBI AGCT 3 cut(s) 79, 434, 666
AluI AGCT 3 cut(s) 79, 434, 666
Alw26I GTCTC 1 cut(s) 372
AlwI GGATC 2 cut(s) 158, 623
AlwNI CAGNNNCTG 2 cut(s) 94, 666
Ama87I CYCGRG 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 666
ApoI RAATTY 2 cut(s) 14, 674
AspLEI GCGC 1 cut(s) 290
AsuHPI GGTGA 3 cut(s) 268, 550, 662
AvaI CYCGRG 1 cut(s) 140
BbsI GAAGAC 1 cut(s) 100
BbvI GCAGC 1 cut(s) 653
BciT130I CCWGG 2 cut(s) 48, 180
BclI TGATCA 1 cut(s) 33
BcoDI GTCTC 1 cut(s) 372
BfaI CTAG 2 cut(s) 80, 347
BfmI CTRYAG 1 cut(s) 429
BisI GCNGC 1 cut(s) 667
BlsI GCNGC 1 cut(s) 668
Bme1390I CCNGG 2 cut(s) 48, 180
BmeT110I CYCGRG 1 cut(s) 140
BmrFI CCNGG 2 cut(s) 48, 180
BmsI GCATC 3 cut(s) 89, 561, 590
BpiI GAAGAC 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 167
BsaWI WCCGGW 2 cut(s) 231, 266
Bsc4I CCNNNNNNNGG 1 cut(s) 273
Bse118I RCCGGY 1 cut(s) 284
Bse1I ACTGG 1 cut(s) 278
BseBI CCWGG 2 cut(s) 48, 180
BseDI CCNNGG 1 cut(s) 167
BseGI GGATG 1 cut(s) 36
BseLI CCNNNNNNNGG 1 cut(s) 273
BseMII CTCAG 2 cut(s) 86, 441
BseNI ACTGG 1 cut(s) 278
BseRI GAGGAG 2 cut(s) 542, 583
BseXI GCAGC 1 cut(s) 653
BsiHKCI CYCGRG 1 cut(s) 140
BsiSI CCGG 3 cut(s) 232, 267, 285
BslI CCNNNNNNNGG 1 cut(s) 273
BsmAI GTCTC 1 cut(s) 372
BsmI GAATGC 2 cut(s) 424, 460
BsoBI CYCGRG 1 cut(s) 140
Bsp143I GATC 4 cut(s) 33, 144, 163, 615
BspCNI CTCAG 2 cut(s) 87, 442
BspPI GGATC 2 cut(s) 158, 623
BspQI GCTCTTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 284
BsrI ACTGG 1 cut(s) 278
BssAI RCCGGY 1 cut(s) 284
BssECI CCNNGG 1 cut(s) 167
BssMI GATC 4 cut(s) 33, 144, 163, 615
BssT1I CCWWGG 1 cut(s) 167
Bst2UI CCWGG 2 cut(s) 48, 180
Bst6I CTCTTC 1 cut(s) 69
BstDEI CTNAG 4 cut(s) 95, 381, 450, 501
BstF5I GGATG 1 cut(s) 36
BstHHI GCGC 1 cut(s) 290
BstKTI GATC 4 cut(s) 36, 147, 166, 618
BstMAI GTCTC 1 cut(s) 372
BstMBI GATC 4 cut(s) 33, 144, 163, 615
BstMWI GCNNNNNNNGC 2 cut(s) 287, 578
BstNI CCWGG 2 cut(s) 48, 180
BstNSI RCATGY 2 cut(s) 160, 377
BstSCI CCNGG 2 cut(s) 46, 178
BstSFI CTRYAG 1 cut(s) 429
BstV1I GCAGC 1 cut(s) 653
BstV2I GAAGAC 1 cut(s) 100
BtsCI GGATG 1 cut(s) 36
CaiI CAGNNNCTG 2 cut(s) 94, 666
CfoI GCGC 1 cut(s) 290
Cfr10I RCCGGY 1 cut(s) 284
Csp6I GTAC 1 cut(s) 91
CviAII CATG 5 cut(s) 114, 157, 374, 397, 401
CviJI RGCY 4 cut(s) 79, 248, 434, 666
CviKI_1 RGCY 4 cut(s) 79, 248, 434, 666
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 4 cut(s) 95, 381, 450, 501
DpnI GATC 4 cut(s) 35, 146, 165, 617
DpnII GATC 4 cut(s) 33, 144, 163, 615
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 193
Eco88I CYCGRG 1 cut(s) 140
EcoRII CCWGG 2 cut(s) 46, 178
EcoT14I CCWWGG 1 cut(s) 167
EcoT22I ATGCAT 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 167
FaeI CATG 5 cut(s) 117, 160, 377, 400, 404
FaiI YATR 7 cut(s) 115, 158, 162, 324, 375, 398, 402
FatI CATG 5 cut(s) 113, 156, 373, 396, 400
FbaI TGATCA 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 667
FokI GGATG 1 cut(s) 43
Fsp4HI GCNGC 1 cut(s) 667
FspBI CTAG 2 cut(s) 80, 347
GlaI GCGC 1 cut(s) 289
GluI GCNGC 1 cut(s) 667
HapII CCGG 3 cut(s) 232, 267, 285
HhaI GCGC 1 cut(s) 290
Hin1II CATG 5 cut(s) 117, 160, 377, 400, 404
Hin6I GCGC 1 cut(s) 288
HinP1I GCGC 1 cut(s) 288
HincII GTYRAC 1 cut(s) 562
HindII GTYRAC 1 cut(s) 562
HinfI GANTC 5 cut(s) 57, 221, 319, 343, 574
HpaI GTTAAC 1 cut(s) 562
HpaII CCGG 3 cut(s) 232, 267, 285
HphI GGTGA 3 cut(s) 268, 550, 662
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 2 cut(s) 38, 56
Hpy188III TCNNGA 1 cut(s) 140
Hpy8I GTNNAC 1 cut(s) 562
HpyCH4IV ACGT 1 cut(s) 150
HpyCH4V TGCA 5 cut(s) 102, 156, 400, 422, 581
HpyF10VI GCNNNNNNNGC 2 cut(s) 287, 578
HpyF3I CTNAG 4 cut(s) 95, 381, 450, 501
HpySE526I ACGT 1 cut(s) 150
Hsp92II CATG 5 cut(s) 117, 160, 377, 400, 404
HspAI GCGC 1 cut(s) 288
Ksp22I TGATCA 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 562
Kzo9I GATC 4 cut(s) 33, 144, 163, 615
LguI GCTCTTC 1 cut(s) 69
Lsp1109I GCAGC 1 cut(s) 653
LweI GCATC 3 cut(s) 89, 561, 590
MaeI CTAG 2 cut(s) 80, 347
MaeII ACGT 1 cut(s) 150
MaeIII GTNAC 3 cut(s) 256, 269, 625
MalI GATC 4 cut(s) 35, 146, 165, 617
MboI GATC 4 cut(s) 33, 144, 163, 615
MboII GAAGA 8 cut(s) 86, 100, 199, 300, 576, 579, 653, 665
MluCI AATT 5 cut(s) 14, 184, 475, 541, 674
MlyI GAGTC 2 cut(s) 230, 337
MmeI TCCRAC 1 cut(s) 85
MnlI CCTC 7 cut(s) 54, 192, 309, 353, 520, 601, 604
Mph1103I ATGCAT 1 cut(s) 402
MseI TTAA 2 cut(s) 461, 561
MspI CCGG 3 cut(s) 232, 267, 285
MspR9I CCNGG 2 cut(s) 48, 180
Mva1269I GAATGC 2 cut(s) 424, 460
MvaI CCWGG 2 cut(s) 48, 180
MwoI GCNNNNNNNGC 2 cut(s) 287, 578
NdeII GATC 4 cut(s) 33, 144, 163, 615
NlaIII CATG 5 cut(s) 117, 160, 377, 400, 404
NmuCI GTSAC 1 cut(s) 256
NsiI ATGCAT 1 cut(s) 402
NspI RCATGY 2 cut(s) 160, 377
PciI ACATGT 1 cut(s) 373
PciSI GCTCTTC 1 cut(s) 69
PctI GAATGC 2 cut(s) 424, 460
PfeI GAWTC 3 cut(s) 57, 319, 574
PkrI GCNGC 1 cut(s) 668
PleI GAGTC 2 cut(s) 229, 337
PpsI GAGTC 2 cut(s) 229, 337
PscI ACATGT 1 cut(s) 373
Psp1406I AACGTT 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 46, 178
PspGI CCWGG 2 cut(s) 46, 178
PstNI CAGNNNCTG 2 cut(s) 94, 666
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 461, 561
SatI GCNGC 1 cut(s) 667
Sau3AI GATC 4 cut(s) 33, 144, 163, 615
SchI GAGTC 2 cut(s) 230, 337
ScrFI CCNGG 2 cut(s) 48, 180
SetI ASST 7 cut(s) 81, 96, 153, 184, 203, 436, 668
SfaNI GCATC 3 cut(s) 89, 561, 590
SfcI CTRYAG 1 cut(s) 429
Sse9I AATT 5 cut(s) 14, 184, 475, 541, 674
SspI AATATT 1 cut(s) 407
SspMI CTAG 2 cut(s) 80, 347
StyD4I CCNGG 2 cut(s) 46, 178
StyI CCWWGG 1 cut(s) 167
TaiI ACGT 1 cut(s) 153
TasI AATT 5 cut(s) 14, 184, 475, 541, 674
TfiI GAWTC 3 cut(s) 57, 319, 574
Tru1I TTAA 2 cut(s) 461, 561
Tru9I TTAA 2 cut(s) 461, 561
TseFI GTSAC 1 cut(s) 256
TseI GCWGC 1 cut(s) 666
Tsp45I GTSAC 1 cut(s) 256
TspDTI ATGAA 3 cut(s) 87, 417, 536
XapI RAATTY 2 cut(s) 14, 674
XceI RCATGY 2 cut(s) 160, 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 474
XspI CTAG 2 cut(s) 80, 347
Zsp2I ATGCAT 1 cut(s) 402
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.