MD17G1010300.v1.1
NAC Family

NAC transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0007675
Physical Location & Seq
Reverse (-)
641271 .. 642512
1242 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1010300.v1.1.491

Sequence Viewer

Length: 795 bp
ATGGAGAATATTAAAGAAAATTATGTAATTTATGGAGGAGTGAAGATGCCGGTTGGGTACCGGTTTCGTCCCACGGACGAAGAGCTGGTGATTCACTACTTGATGAGGAAGGTTCATGCTGCCCCATTGCCTGCTTCCATCATTCCAGAGTTTGATGTCTTCGAAACTCATCCCTGGGGCTTGCCAGGTGATATTAGGGAAACAAGGTATTTCTTTTATAATGAAAATATGAATAAGTATGTTGGCATCAACAGCAAGAGAGCTGCTGGTTGTGGGTACTGGAAACCTATTGGCAAAAAGAAGCAAATTGTTGCTAATTCTAAATCCAATGAAGCCGTTGGGATCCGGAAGACGTTGGGTTTCTGTGAAAGGAAGCGCCGCCGTTGCCACAACCACTACCACGAGAGTACTAGTACTACCACTCAAACTCGATGGCTCATGCATGAATACCGCCTCTTGGCTTGTCAAGCGAATCCAACCCAGGCTTTCAAAGCGGAAGTGGGAAACTGGGTGGTGTTTCGCATATTTCAGAAAAAGAGAAGGCCTACTCAAAGAATATCAGATGACATGGGAGTAGTTTCTCAACGCTCATCAAACTGCAAGAGGAATCGAAGATTAATGGAGGAATATATCTCTGCAGCTGATCATCGTTCACGACGTTCGGATCCTGATCTTACAACACTTTCTTCCCCATCATGCTCGAGTGATATCACTCATGAGCTCACTTCCAATGACAATGGCGAGCTAGACGATGATCAGGAGGGACACAGTGTTAGCGTGCGGATTAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

265

Amino Acids

30.58

Weight (kDa)

9.34

Isoelectric Point (pI)

59.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 17 - 152 3.1e-32 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 219
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 57
AccIII TCCGGA 1 cut(s) 345
AciI CCGC 4 cut(s) 379, 451, 494, 781
AclWI GGATC 4 cut(s) 337, 350, 659, 672
AfaI GTAC 4 cut(s) 59, 278, 409, 415
AfiI CCNNNNNNNGG 1 cut(s) 457
AgeI ACCGGT 1 cut(s) 60
AgsI TTSAA 1 cut(s) 490
AhlI ACTAGT 1 cut(s) 410
AjnI CCWGG 3 cut(s) 173, 184, 480
AluBI AGCT 5 cut(s) 85, 263, 641, 721, 745
AluI AGCT 5 cut(s) 85, 263, 641, 721, 745
Alw21I GWGCWC 1 cut(s) 723
AlwI GGATC 4 cut(s) 337, 350, 659, 672
Ama87I CYCGRG 1 cut(s) 700
Aor13HI TCCGGA 1 cut(s) 345
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 3 cut(s) 119, 263, 638
AseI ATTAAT 2 cut(s) 617, 786
AsiGI ACCGGT 1 cut(s) 60
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 378
AsuHPI GGTGA 2 cut(s) 100, 200
AsuII TTCGAA 1 cut(s) 162
AvaI CYCGRG 1 cut(s) 700
BamHI GGATCC 2 cut(s) 342, 664
BanI GGYRCC 1 cut(s) 57
BanII GRGCYC 1 cut(s) 723
BauI CACGAG 1 cut(s) 401
BbsI GAAGAC 2 cut(s) 151, 356
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 3 cut(s) 106, 250, 650
BccI CCATC 3 cut(s) 146, 426, 700
BceAI ACGGC 2 cut(s) 320, 366
BciT130I CCWGG 3 cut(s) 175, 186, 482
BclI TGATCA 2 cut(s) 643, 754
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 2 cut(s) 411, 746
BfmI CTRYAG 1 cut(s) 636
BfoI RGCGCY 1 cut(s) 379
BisI GCNGC 4 cut(s) 120, 264, 379, 639
BlsI GCNGC 4 cut(s) 121, 265, 380, 640
BmcAI AGTACT 2 cut(s) 409, 415
Bme1390I CCNGG 3 cut(s) 175, 186, 482
BmeT110I CYCGRG 1 cut(s) 700
BmiI GGNNCC 3 cut(s) 59, 344, 666
BmrFI CCNGG 3 cut(s) 175, 186, 482
BmrI ACTGGG 1 cut(s) 517
BmsI GCATC 2 cut(s) 36, 255
BmuI ACTGGG 1 cut(s) 517
BpiI GAAGAC 2 cut(s) 151, 356
Bpu14I TTCGAA 1 cut(s) 162
BsaBI GATNNNNATC 1 cut(s) 669
BsaJI CCNNGG 4 cut(s) 72, 173, 174, 480
BsaWI WCCGGW 2 cut(s) 60, 345
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse118I RCCGGY 2 cut(s) 49, 60
Bse1I ACTGG 2 cut(s) 284, 512
Bse3DI GCAATG 1 cut(s) 125
Bse8I GATNNNNATC 1 cut(s) 669
BseAI TCCGGA 1 cut(s) 345
BseBI CCWGG 3 cut(s) 175, 186, 482
BseDI CCNNGG 4 cut(s) 72, 173, 174, 480
BseGI GGATG 1 cut(s) 169
BseJI GATNNNNATC 1 cut(s) 669
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 1 cut(s) 125
BseNI ACTGG 2 cut(s) 284, 512
BseRI GAGGAG 1 cut(s) 51
BseXI GCAGC 3 cut(s) 106, 250, 650
BshFI GGCC 1 cut(s) 544
BshNI GGYRCC 1 cut(s) 57
BshTI ACCGGT 1 cut(s) 60
BsiHKAI GWGCWC 1 cut(s) 723
BsiHKCI CYCGRG 1 cut(s) 700
BsiSI CCGG 3 cut(s) 50, 61, 346
BslFI GGGAC 2 cut(s) 54, 777
BslI CCNNNNNNNGG 1 cut(s) 457
BsmFI GGGAC 2 cut(s) 54, 777
BsnI GGCC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 700
Bsp119I TTCGAA 1 cut(s) 162
Bsp1286I GDGCHC 1 cut(s) 723
Bsp13I TCCGGA 1 cut(s) 345
Bsp143I GATC 5 cut(s) 342, 643, 664, 670, 754
BspACI CCGC 4 cut(s) 379, 451, 494, 781
BspANI GGCC 1 cut(s) 544
BspEI TCCGGA 1 cut(s) 345
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 3 cut(s) 59, 344, 666
BspMAI CTGCAG 1 cut(s) 640
BspPI GGATC 4 cut(s) 337, 350, 659, 672
BspQI GCTCTTC 1 cut(s) 75
BspT104I TTCGAA 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 125
BsrFI RCCGGY 2 cut(s) 49, 60
BsrI ACTGG 2 cut(s) 284, 512
BssAI RCCGGY 2 cut(s) 49, 60
BssECI CCNNGG 4 cut(s) 72, 173, 174, 480
BssMI GATC 5 cut(s) 342, 643, 664, 670, 754
BssSI CACGAG 1 cut(s) 401
Bst2BI CACGAG 1 cut(s) 401
Bst2UI CCWGG 3 cut(s) 175, 186, 482
Bst4CI ACNGT 1 cut(s) 770
Bst6I CTCTTC 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 162
BstC8I GCNNGC 4 cut(s) 132, 182, 743, 779
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 1 cut(s) 169
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 5 cut(s) 345, 646, 667, 673, 757
BstMBI GATC 5 cut(s) 342, 643, 664, 670, 754
BstMWI GCNNNNNNNGC 4 cut(s) 252, 384, 467, 491
BstNI CCWGG 3 cut(s) 175, 186, 482
BstSCI CCNGG 3 cut(s) 173, 184, 480
BstSFI CTRYAG 1 cut(s) 636
BstV1I GCAGC 3 cut(s) 106, 250, 650
BstV2I GAAGAC 2 cut(s) 151, 356
BstX2I RGATCY 2 cut(s) 342, 664
BstYI RGATCY 2 cut(s) 342, 664
BsuRI GGCC 1 cut(s) 544
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 775
Cac8I GCNNGC 4 cut(s) 132, 182, 743, 779
CciI TCATGA 1 cut(s) 715
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 2 cut(s) 49, 60
Csp6I GTAC 4 cut(s) 58, 277, 408, 414
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 6 cut(s) 116, 439, 443, 568, 696, 716
CviQI GTAC 4 cut(s) 58, 277, 408, 414
DpnI GATC 5 cut(s) 344, 645, 666, 672, 756
DpnII GATC 5 cut(s) 342, 643, 664, 670, 754
Eam1104I CTCTTC 1 cut(s) 75
EarI CTCTTC 1 cut(s) 75
Ecl136II GAGCTC 1 cut(s) 721
Eco147I AGGCCT 1 cut(s) 544
Eco24I GRGCYC 1 cut(s) 723
Eco32I GATATC 1 cut(s) 709
Eco53kI GAGCTC 1 cut(s) 721
Eco88I CYCGRG 1 cut(s) 700
EcoICRI GAGCTC 1 cut(s) 721
EcoRII CCWGG 3 cut(s) 173, 184, 480
EcoRV GATATC 1 cut(s) 709
EcoT22I ATGCAT 1 cut(s) 444
EcoT38I GRGCYC 1 cut(s) 723
FaeI CATG 6 cut(s) 119, 442, 446, 571, 699, 719
FaqI GGGAC 2 cut(s) 54, 777
FatI CATG 6 cut(s) 115, 438, 442, 567, 695, 715
FbaI TGATCA 2 cut(s) 643, 754
Fnu4HI GCNGC 4 cut(s) 120, 264, 379, 639
FokI GGATG 1 cut(s) 156
FriOI GRGCYC 1 cut(s) 723
Fsp4HI GCNGC 4 cut(s) 120, 264, 379, 639
FspBI CTAG 2 cut(s) 411, 746
GlaI GCGC 1 cut(s) 377
GluI GCNGC 4 cut(s) 120, 264, 379, 639
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 1 cut(s) 544
HapII CCGG 3 cut(s) 50, 61, 346
HhaI GCGC 1 cut(s) 378
Hin1II CATG 6 cut(s) 119, 442, 446, 571, 699, 719
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 3 cut(s) 91, 472, 607
HpaII CCGG 3 cut(s) 50, 61, 346
HphI GGTGA 2 cut(s) 100, 200
Hpy166II GTNNAC 1 cut(s) 653
Hpy188I TCNGA 3 cut(s) 531, 562, 664
Hpy188III TCNNGA 6 cut(s) 146, 346, 654, 668, 716, 758
Hpy8I GTNNAC 1 cut(s) 653
Hpy99I CGWCG 1 cut(s) 660
HpyAV CCTTC 2 cut(s) 103, 534
HpyCH4III ACNGT 1 cut(s) 770
HpyCH4IV ACGT 2 cut(s) 353, 658
HpyCH4V TGCA 3 cut(s) 442, 600, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 252, 384, 467, 491
HpySE526I ACGT 2 cut(s) 353, 658
Hsp92II CATG 6 cut(s) 119, 442, 446, 571, 699, 719
HspAI GCGC 1 cut(s) 376
Kpn2I TCCGGA 1 cut(s) 345
KpnI GGTACC 1 cut(s) 61
Ksp22I TGATCA 2 cut(s) 643, 754
Kzo9I GATC 5 cut(s) 342, 643, 664, 670, 754
LguI GCTCTTC 1 cut(s) 75
Lsp1109I GCAGC 3 cut(s) 106, 250, 650
LweI GCATC 2 cut(s) 36, 255
MaeI CTAG 2 cut(s) 411, 746
MaeII ACGT 2 cut(s) 353, 658
MalI GATC 5 cut(s) 344, 645, 666, 672, 756
MboI GATC 5 cut(s) 342, 643, 664, 670, 754
MboII GAAGA 6 cut(s) 55, 92, 151, 361, 624, 678
MflI RGATCY 2 cut(s) 342, 664
MhlI GDGCHC 1 cut(s) 723
MluCI AATT 4 cut(s) 19, 27, 306, 316
MmeI TCCRAC 1 cut(s) 500
MnlI CCTC 6 cut(s) 29, 99, 464, 597, 616, 754
Mph1103I ATGCAT 1 cut(s) 444
MroI TCCGGA 1 cut(s) 345
MseI TTAA 3 cut(s) 12, 617, 786
MspA1I CMGCKG 1 cut(s) 641
MspI CCGG 3 cut(s) 50, 61, 346
MspR9I CCNGG 3 cut(s) 175, 186, 482
MvaI CCWGG 3 cut(s) 175, 186, 482
MwoI GCNNNNNNNGC 4 cut(s) 252, 384, 467, 491
NdeII GATC 5 cut(s) 342, 643, 664, 670, 754
NlaIII CATG 6 cut(s) 119, 442, 446, 571, 699, 719
NlaIV GGNNCC 3 cut(s) 59, 344, 666
NsiI ATGCAT 1 cut(s) 444
NspV TTCGAA 1 cut(s) 162
PaeR7I CTCGAG 1 cut(s) 700
PagI TCATGA 1 cut(s) 715
PasI CCCWGGG 1 cut(s) 174
PceI AGGCCT 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 1 cut(s) 655
PfeI GAWTC 3 cut(s) 91, 472, 607
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 4 cut(s) 121, 265, 380, 640
PshBI ATTAAT 2 cut(s) 617, 786
PsiI TTATAA 1 cut(s) 219
Psp124BI GAGCTC 1 cut(s) 723
Psp6I CCWGG 3 cut(s) 173, 184, 480
PspGI CCWGG 3 cut(s) 173, 184, 480
PspN4I GGNNCC 3 cut(s) 59, 344, 666
PspXI VCTCGAGB 1 cut(s) 700
PstI CTGCAG 1 cut(s) 640
PsuI RGATCY 2 cut(s) 342, 664
PvuII CAGCTG 1 cut(s) 641
RsaI GTAC 4 cut(s) 59, 278, 409, 415
RsaNI GTAC 4 cut(s) 58, 277, 408, 414
SacI GAGCTC 1 cut(s) 723
SapI GCTCTTC 1 cut(s) 75
SaqAI TTAA 3 cut(s) 12, 617, 786
SatI GCNGC 4 cut(s) 120, 264, 379, 639
Sau3AI GATC 5 cut(s) 342, 643, 664, 670, 754
ScaI AGTACT 2 cut(s) 409, 415
ScrFI CCNGG 3 cut(s) 175, 186, 482
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 2 cut(s) 36, 255
SfcI CTRYAG 1 cut(s) 636
Sfr274I CTCGAG 1 cut(s) 700
SfuI TTCGAA 1 cut(s) 162
SlaI CTCGAG 1 cut(s) 700
SmlI CTYRAG 1 cut(s) 700
SmoI CTYRAG 1 cut(s) 700
SpeI ACTAGT 1 cut(s) 410
Sse9I AATT 4 cut(s) 19, 27, 306, 316
SseBI AGGCCT 1 cut(s) 544
SsiI CCGC 4 cut(s) 379, 451, 494, 781
SspI AATATT 2 cut(s) 10, 790
SspMI CTAG 2 cut(s) 411, 746
SstI GAGCTC 1 cut(s) 723
StuI AGGCCT 1 cut(s) 544
StyD4I CCNGG 3 cut(s) 173, 184, 480
TaaI ACNGT 1 cut(s) 770
TaiI ACGT 2 cut(s) 356, 661
TaqI TCGA 4 cut(s) 162, 430, 610, 701
TasI AATT 4 cut(s) 19, 27, 306, 316
TatI WGTACW 2 cut(s) 407, 413
TauI GCSGC 1 cut(s) 381
TfiI GAWTC 3 cut(s) 91, 472, 607
Tru1I TTAA 3 cut(s) 12, 617, 786
Tru9I TTAA 3 cut(s) 12, 617, 786
TscAI CASTG 1 cut(s) 775
TseI GCWGC 3 cut(s) 119, 263, 638
TspDTI ATGAA 5 cut(s) 104, 237, 245, 345, 459
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 775
VspI ATTAAT 2 cut(s) 617, 786
XhoI CTCGAG 1 cut(s) 700
XspI CTAG 2 cut(s) 411, 746
ZrmI AGTACT 2 cut(s) 409, 415
Zsp2I ATGCAT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.