pycom111g00470
NAC Family

NAC transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
SuperScaffold_111
OG0007675
Physical Location & Seq
Reverse (-)
339785 .. 340793
1009 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom111g00470.1

Sequence Viewer

Length: 792 bp
ATGGAAAATATTAGAGAAAACTATGTTATAAATGGAGGAGTGAAGATGCCAGTTGGGTACCGGTTTCGTCCCACGGATGAGGAGCTGGTGATTCACTACTTGATGAGGAAGGTTCATGCCGCCCCATTGCCTGCTTCCATCATTCCAGAGTTCGATGTCTTCGAAACTCATCCCTGGGGCTTGCCAGGTGATGTTAGGGAAAAAAGGTATTTCTTTTACAATGAAAATATGAATAAGAATGTTGGCATCAACAGCAGGAGAGCTGCAGGATGTGGGTACTGGAAGCCTATTGGCAAAAAGAAGCAGATTGTTAATAATTCTGAATCCAAGGAAGCCGTTGGGATCCGGAAGACGTTGGTTTTCTGTGAAAGGAAGCGCCGCCGTTGCCACCACCACCACCATAGGAGTACTGATACTACCACTCAAACTCGATGGCTCATGCATGAATACCGCCTGTTGGCTTCTCAAGCAAATCCAACCCAGGTTTTGGAAAAGGAATCCGGGAACTGGGTGGTCTGTCGCATATTTCAGAAAAAGAGAAGGCCTACTCAAAGAACATCAGATGATATGGGGGTCGTTTCTGAACGCTCATCAAACTGCAACAAGACTCGGAGATTAATGGGGGATTATATCTCTGCAGATGATCATTCACGACGTTCGGATCCTGATCTTACAACACTTTCTTCCCCATCATGCTCGAGTGACATCACTCGTGAGTTCTCTTCGAATGACAATGGTGAGCTAGACGATGATCAGGAGGGACACAGTGTTAGCATGCCGATTAATATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAM

Protein Analysis

264

Amino Acids

30.43

Weight (kDa)

9.11

Isoelectric Point (pI)

60.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 17 - 152 5.3e-34 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 29
Acc65I GGTACC 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 57
AccB7I CCANNNNNTGG 1 cut(s) 487
AccIII TCCGGA 1 cut(s) 345
AciI CCGC 3 cut(s) 120, 379, 451
AclWI GGATC 4 cut(s) 337, 350, 656, 669
AfaI GTAC 3 cut(s) 59, 278, 409
AfiI CCNNNNNNNGG 3 cut(s) 457, 487, 507
AgeI ACCGGT 1 cut(s) 60
AjnI CCWGG 3 cut(s) 173, 184, 480
AluBI AGCT 3 cut(s) 85, 263, 742
AluI AGCT 3 cut(s) 85, 263, 742
AlwI GGATC 4 cut(s) 337, 350, 656, 669
Ama87I CYCGRG 1 cut(s) 697
Aor13HI TCCGGA 1 cut(s) 345
AoxI GGCC 1 cut(s) 542
ApeKI GCWGC 1 cut(s) 263
AseI ATTAAT 2 cut(s) 617, 783
AsiGI ACCGGT 1 cut(s) 60
Asp718I GGTACC 1 cut(s) 57
AspLEI GCGC 1 cut(s) 378
AsuC2I CCSGG 1 cut(s) 502
AsuHPI GGTGA 3 cut(s) 100, 200, 749
AsuII TTCGAA 2 cut(s) 162, 725
AvaI CYCGRG 1 cut(s) 697
BamHI GGATCC 2 cut(s) 342, 661
BanI GGYRCC 1 cut(s) 57
BauI CACGAG 1 cut(s) 711
BbsI GAAGAC 2 cut(s) 151, 356
BbvI GCAGC 1 cut(s) 250
BccI CCATC 3 cut(s) 146, 426, 697
BceAI ACGGC 2 cut(s) 320, 366
BciT130I CCWGG 3 cut(s) 175, 186, 482
BclI TGATCA 2 cut(s) 643, 751
BcnI CCSGG 1 cut(s) 502
BfaI CTAG 1 cut(s) 743
BfmI CTRYAG 2 cut(s) 264, 636
BfoI RGCGCY 1 cut(s) 379
BisI GCNGC 3 cut(s) 120, 264, 379
BlsI GCNGC 3 cut(s) 121, 265, 380
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 4 cut(s) 175, 186, 482, 502
BmeT110I CYCGRG 1 cut(s) 697
BmiI GGNNCC 3 cut(s) 59, 344, 663
BmrFI CCNGG 4 cut(s) 175, 186, 482, 502
BmrI ACTGGG 1 cut(s) 517
BmsI GCATC 2 cut(s) 36, 255
BmuI ACTGGG 1 cut(s) 517
BpiI GAAGAC 2 cut(s) 151, 356
Bpu14I TTCGAA 2 cut(s) 162, 725
BpuEI CTTGAG 1 cut(s) 450
BpuMI CCSGG 1 cut(s) 502
BsaBI GATNNNNATC 1 cut(s) 666
BsaJI CCNNGG 5 cut(s) 72, 173, 174, 327, 480
BsaWI WCCGGW 2 cut(s) 60, 345
Bsc4I CCNNNNNNNGG 3 cut(s) 457, 487, 507
Bse118I RCCGGY 1 cut(s) 60
Bse1I ACTGG 3 cut(s) 50, 284, 512
Bse3DI GCAATG 1 cut(s) 125
Bse8I GATNNNNATC 1 cut(s) 666
BseAI TCCGGA 1 cut(s) 345
BseBI CCWGG 3 cut(s) 175, 186, 482
BseDI CCNNGG 5 cut(s) 72, 173, 174, 327, 480
BseGI GGATG 3 cut(s) 82, 169, 275
BseJI GATNNNNATC 1 cut(s) 666
BseLI CCNNNNNNNGG 3 cut(s) 457, 487, 507
BseMI GCAATG 1 cut(s) 125
BseNI ACTGG 3 cut(s) 50, 284, 512
BseRI GAGGAG 2 cut(s) 51, 95
BseXI GCAGC 1 cut(s) 250
BshFI GGCC 1 cut(s) 544
BshNI GGYRCC 1 cut(s) 57
BshTI ACCGGT 1 cut(s) 60
BsiHKCI CYCGRG 1 cut(s) 697
BsiSI CCGG 3 cut(s) 61, 346, 501
BslFI GGGAC 2 cut(s) 54, 774
BslI CCNNNNNNNGG 3 cut(s) 457, 487, 507
BsmFI GGGAC 2 cut(s) 54, 774
BsnI GGCC 1 cut(s) 544
BsoBI CYCGRG 1 cut(s) 697
Bsp119I TTCGAA 2 cut(s) 162, 725
Bsp13I TCCGGA 1 cut(s) 345
Bsp143I GATC 5 cut(s) 342, 643, 661, 667, 751
BspACI CCGC 3 cut(s) 120, 379, 451
BspANI GGCC 1 cut(s) 544
BspEI TCCGGA 1 cut(s) 345
BspLI GGNNCC 3 cut(s) 59, 344, 663
BspMAI CTGCAG 2 cut(s) 268, 640
BspPI GGATC 4 cut(s) 337, 350, 656, 669
BspT104I TTCGAA 2 cut(s) 162, 725
BspT107I GGYRCC 1 cut(s) 57
BsrDI GCAATG 1 cut(s) 125
BsrFI RCCGGY 1 cut(s) 60
BsrI ACTGG 3 cut(s) 50, 284, 512
BssAI RCCGGY 1 cut(s) 60
BssECI CCNNGG 5 cut(s) 72, 173, 174, 327, 480
BssMI GATC 5 cut(s) 342, 643, 661, 667, 751
BssSI CACGAG 1 cut(s) 711
BssT1I CCWWGG 1 cut(s) 327
Bst2BI CACGAG 1 cut(s) 711
Bst2UI CCWGG 3 cut(s) 175, 186, 482
Bst4CI ACNGT 1 cut(s) 767
Bst6I CTCTTC 1 cut(s) 727
BstBI TTCGAA 2 cut(s) 162, 725
BstC8I GCNNGC 3 cut(s) 132, 182, 776
BstDSI CCRYGG 1 cut(s) 72
BstF5I GGATG 3 cut(s) 82, 169, 275
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 5 cut(s) 345, 646, 664, 670, 754
BstMBI GATC 5 cut(s) 342, 643, 661, 667, 751
BstMWI GCNNNNNNNGC 3 cut(s) 252, 384, 467
BstNI CCWGG 3 cut(s) 175, 186, 482
BstNSI RCATGY 1 cut(s) 778
BstSCI CCNGG 4 cut(s) 173, 184, 480, 500
BstSFI CTRYAG 2 cut(s) 264, 636
BstV1I GCAGC 1 cut(s) 250
BstV2I GAAGAC 2 cut(s) 151, 356
BstX2I RGATCY 2 cut(s) 342, 661
BstYI RGATCY 2 cut(s) 342, 661
BsuRI GGCC 1 cut(s) 544
BtgI CCRYGG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 82, 169, 275
BtsIMutI CAGTG 1 cut(s) 772
Cac8I GCNNGC 3 cut(s) 132, 182, 776
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 60
Csp6I GTAC 3 cut(s) 58, 277, 408
CspAI ACCGGT 1 cut(s) 60
CviAII CATG 5 cut(s) 116, 439, 443, 693, 775
CviJI RGCY 9 cut(s) 85, 180, 263, 286, 335, 436, 461, 544, 742
CviKI_1 RGCY 9 cut(s) 85, 180, 263, 286, 335, 436, 461, 544, 742
CviQI GTAC 3 cut(s) 58, 277, 408
DpnI GATC 5 cut(s) 344, 645, 663, 669, 753
DpnII GATC 5 cut(s) 342, 643, 661, 667, 751
Eam1104I CTCTTC 1 cut(s) 727
EarI CTCTTC 1 cut(s) 727
Eco130I CCWWGG 1 cut(s) 327
Eco147I AGGCCT 1 cut(s) 544
Eco88I CYCGRG 1 cut(s) 697
EcoRII CCWGG 3 cut(s) 173, 184, 480
EcoT14I CCWWGG 1 cut(s) 327
EcoT22I ATGCAT 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 5 cut(s) 119, 442, 446, 696, 778
FaqI GGGAC 2 cut(s) 54, 774
FatI CATG 5 cut(s) 115, 438, 442, 692, 774
FbaI TGATCA 2 cut(s) 643, 751
Fnu4HI GCNGC 3 cut(s) 120, 264, 379
FokI GGATG 3 cut(s) 89, 156, 282
Fsp4HI GCNGC 3 cut(s) 120, 264, 379
FspBI CTAG 1 cut(s) 743
GlaI GCGC 1 cut(s) 377
GluI GCNGC 3 cut(s) 120, 264, 379
HaeII RGCGCY 1 cut(s) 379
HaeIII GGCC 1 cut(s) 544
HapII CCGG 3 cut(s) 61, 346, 501
HhaI GCGC 1 cut(s) 378
Hin1II CATG 5 cut(s) 119, 442, 446, 696, 778
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HinfI GANTC 4 cut(s) 91, 323, 497, 607
HpaII CCGG 3 cut(s) 61, 346, 501
HphI GGTGA 3 cut(s) 100, 200, 749
Hpy188I TCNGA 6 cut(s) 322, 531, 562, 583, 612, 661
Hpy188III TCNNGA 6 cut(s) 146, 346, 651, 665, 713, 755
Hpy99I CGWCG 1 cut(s) 657
HpyAV CCTTC 2 cut(s) 103, 534
HpyCH4III ACNGT 1 cut(s) 767
HpyCH4IV ACGT 2 cut(s) 353, 655
HpyCH4V TGCA 4 cut(s) 266, 442, 600, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 252, 384, 467
HpySE526I ACGT 2 cut(s) 353, 655
Hsp92II CATG 5 cut(s) 119, 442, 446, 696, 778
HspAI GCGC 1 cut(s) 376
Kpn2I TCCGGA 1 cut(s) 345
KpnI GGTACC 1 cut(s) 61
Ksp22I TGATCA 2 cut(s) 643, 751
Kzo9I GATC 5 cut(s) 342, 643, 661, 667, 751
LmnI GCTCC 1 cut(s) 82
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 2 cut(s) 36, 255
MaeI CTAG 1 cut(s) 743
MaeII ACGT 2 cut(s) 353, 655
MaeIII GTNAC 1 cut(s) 701
MalI GATC 5 cut(s) 344, 645, 663, 669, 753
MboI GATC 5 cut(s) 342, 643, 661, 667, 751
MboII GAAGA 5 cut(s) 55, 151, 361, 675, 714
MflI RGATCY 2 cut(s) 342, 661
MluCI AATT 1 cut(s) 316
MlyI GAGTC 1 cut(s) 601
MmeI TCCRAC 1 cut(s) 500
MnlI CCTC 4 cut(s) 29, 73, 99, 751
Mph1103I ATGCAT 1 cut(s) 444
MroI TCCGGA 1 cut(s) 345
MseI TTAA 3 cut(s) 312, 617, 783
MspI CCGG 3 cut(s) 61, 346, 501
MspR9I CCNGG 4 cut(s) 175, 186, 482, 502
MvaI CCWGG 3 cut(s) 175, 186, 482
MwoI GCNNNNNNNGC 3 cut(s) 252, 384, 467
NciI CCSGG 1 cut(s) 502
NdeII GATC 5 cut(s) 342, 643, 661, 667, 751
NlaIII CATG 5 cut(s) 119, 442, 446, 696, 778
NlaIV GGNNCC 3 cut(s) 59, 344, 663
NmuCI GTSAC 1 cut(s) 701
NsiI ATGCAT 1 cut(s) 444
NspI RCATGY 1 cut(s) 778
NspV TTCGAA 2 cut(s) 162, 725
PaeI GCATGC 1 cut(s) 778
PaeR7I CTCGAG 1 cut(s) 697
PasI CCCWGGG 1 cut(s) 174
PceI AGGCCT 1 cut(s) 544
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 3 cut(s) 91, 323, 497
PflMI CCANNNNNTGG 1 cut(s) 487
PfoI TCCNGGA 1 cut(s) 500
PinAI ACCGGT 1 cut(s) 60
PkrI GCNGC 3 cut(s) 121, 265, 380
PleI GAGTC 1 cut(s) 601
PpsI GAGTC 1 cut(s) 601
PshBI ATTAAT 2 cut(s) 617, 783
PsiI TTATAA 1 cut(s) 29
Psp6I CCWGG 3 cut(s) 173, 184, 480
PspGI CCWGG 3 cut(s) 173, 184, 480
PspN4I GGNNCC 3 cut(s) 59, 344, 663
PspXI VCTCGAGB 1 cut(s) 697
PstI CTGCAG 2 cut(s) 268, 640
PsuI RGATCY 2 cut(s) 342, 661
RsaI GTAC 3 cut(s) 59, 278, 409
RsaNI GTAC 3 cut(s) 58, 277, 408
SaqAI TTAA 3 cut(s) 312, 617, 783
SatI GCNGC 3 cut(s) 120, 264, 379
Sau3AI GATC 5 cut(s) 342, 643, 661, 667, 751
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 1 cut(s) 601
ScrFI CCNGG 4 cut(s) 175, 186, 482, 502
SetI ASST 9 cut(s) 87, 114, 190, 209, 265, 356, 486, 658, 744
SfaNI GCATC 2 cut(s) 36, 255
SfcI CTRYAG 2 cut(s) 264, 636
Sfr274I CTCGAG 1 cut(s) 697
SfuI TTCGAA 2 cut(s) 162, 725
SlaI CTCGAG 1 cut(s) 697
SmlI CTYRAG 2 cut(s) 465, 697
SmoI CTYRAG 2 cut(s) 465, 697
SphI GCATGC 1 cut(s) 778
Sse9I AATT 1 cut(s) 316
SseBI AGGCCT 1 cut(s) 544
SsiI CCGC 3 cut(s) 120, 379, 451
SspI AATATT 2 cut(s) 10, 787
SspMI CTAG 1 cut(s) 743
StuI AGGCCT 1 cut(s) 544
StyD4I CCNGG 4 cut(s) 173, 184, 480, 500
StyI CCWWGG 1 cut(s) 327
TaaI ACNGT 1 cut(s) 767
TaiI ACGT 2 cut(s) 356, 658
TaqI TCGA 5 cut(s) 153, 162, 430, 698, 725
TasI AATT 1 cut(s) 316
TatI WGTACW 1 cut(s) 407
TauI GCSGC 2 cut(s) 122, 381
TfiI GAWTC 3 cut(s) 91, 323, 497
Tru1I TTAA 3 cut(s) 312, 617, 783
Tru9I TTAA 3 cut(s) 312, 617, 783
TscAI CASTG 1 cut(s) 772
TseFI GTSAC 1 cut(s) 701
TseI GCWGC 1 cut(s) 263
Tsp45I GTSAC 1 cut(s) 701
TspDTI ATGAA 4 cut(s) 104, 237, 245, 459
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 1 cut(s) 772
Van91I CCANNNNNTGG 1 cut(s) 487
VspI ATTAAT 2 cut(s) 617, 783
XceI RCATGY 1 cut(s) 778
XhoI CTCGAG 1 cut(s) 697
XspI CTAG 1 cut(s) 743
ZrmI AGTACT 1 cut(s) 409
Zsp2I ATGCAT 1 cut(s) 444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.