Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004476

Physical Location & Seq

Genomic Coordinates Forward (+)

30991014 .. 30994951

Gene Structure 3938 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1169300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1287 bp

ATGGCCGGCGCCGCCTCGGCGCTTTTTCTGCTCGACATCAAAGGACGAGTTTTGATTTGGCGCGACTACCGTGGCGATGTCCCTGCTGCTCAGGCCGAACGCTTCTTCACCAAGTTCATCGAAAAAGAGGGCGATCCGCAATCCCAAGATCCAGTGGTGTACGACAATGGAGTCAGCTACATGTTCATACAACACAACAATATCTACTTGATGATTGCGTCGAGGCAGAACTGCAATGCTGCTAGCTTGCTGCTGTTTCTGCACCGCGTAATCGATGTTTTTAAGCATTATTTTGAAGAGCTAGAAGAGGAATCGCTAAGAGACAACTTTGTCGTAGTGTACGAGTTACTTGATGAAATTATGGACTTTGGCTTCCCGCAATTCACTGAAGCGAAGATTCTTAGTGAATTTATCAAGACCGATGCTTACAGGATGGAGGTGACACAACGTCCTCCCATGGCCGTTACAAATGCAGTGTCTTGGCGCAGTGAAGGGATACGGTACAAGAAGAACGAAGTCTTTTTGGATGTGGTGGAAAGTGTCAACATACTTGTCAACAGCATCGGGCAAATAATACGGTCAGATGTCGTTGGGGCCCTAAAGATGAGAACATATTTGAGTGGTATGCCCGAGTGTAAGCTTGGACTAAATGATAGAGTGCTGCTAGAAGCGCAAGGTCAAACATCGAAAGGAAAATCCATTGATCTGGATGACATTAAGTTCCATCAGTGTGTGCGTTTGTCTCGATTTGAGAATGACCGGACTATATCGTTCATCCCCCCTGATGGAGCATTTGATCTGATGACATACAGACTCAGTACTCAGGTGAAGCCTCTTATCTGGGTGGAAGCACAAGTTGAAAATCATTCTAGATGTCGTATTGAATTCACGGTAAAAGCAAGGAGCCAGTATAAGGATCGCAGCACGGCCACAAATGTCGAAATTGAGTTGCCTGTGCCCGCTGATGCTACAAACCCTAATGTCCGGACATCAATAGGATCTGCTGCATATGCTCCTGAAAGAGATGCTTTGGTCTGGAAAATTAAGTCTTTTCCTGGTAATAAGGAATACATGTTGAGATCGGAGTTTACACTTCCTAGTATAACCGCTGAAGAAGCGGTTCCCGAAAGAAGAGCTCCTATTCGGGTGAAGTTTGAGATACCGTATTTTACTGTCTCCGGAATTCAGGTCCGATACCTAAAAATCATTGAGAAAAGTGGGTACCAGGCTCTTCCATGGGTGAGATACATAACAATGGCTGGCGAATACGAACTGAGACTAACGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0000323 GO:0001775 GO:0002252 GO:0002263 GO:0002274 GO:0002275 GO:0002283 GO:0002366 GO:0002376 GO:0002443 GO:0002444 GO:0002446 GO:0002478 GO:0002495 GO:0002504 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005765 GO:0005773 GO:0005774 GO:0005794 GO:0005802 GO:0005829 GO:0005886 GO:0006810 GO:0006887 GO:0006955 GO:0006996 GO:0008150 GO:0009605 GO:0009987 GO:0012505 GO:0012506 GO:0016020 GO:0016032 GO:0016043 GO:0016050 GO:0016192 GO:0019882 GO:0019884 GO:0019886 GO:0021700 GO:0030135 GO:0030136 GO:0030141 GO:0030154 GO:0030659 GO:0030662 GO:0030665 GO:0030667 GO:0031090 GO:0031410 GO:0031982 GO:0031984 GO:0032438 GO:0032502 GO:0032588 GO:0032940 GO:0033059 GO:0035579 GO:0035646 GO:0036230 GO:0042119 GO:0042581 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043299 GO:0043312 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044403 GO:0044419 GO:0044422 GO:0044424 GO:0044431 GO:0044433 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0045055 GO:0045321 GO:0046903 GO:0046907 GO:0048002 GO:0048066 GO:0048753 GO:0048757 GO:0048869 GO:0050690 GO:0050896 GO:0050931 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051704 GO:0051716 GO:0071840 GO:0071944 GO:0097708 GO:0098588 GO:0098791 GO:0098805 GO:0098852 GO:0099503

KEGG Pathways

Metabolic & Signaling

ko04142 map04142

Pfam Domains

Protein Families

Adap_comp_sub Clat_adaptor_s

Protein Analysis

429

Amino Acids

49.13

Weight (kDa)

5.92

Isoelectric Point (pI)

41.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Clat_adaptor_s	PF01217	7 - 126	5.1e-06	Clathrin adaptor complex small chain
Adap_comp_sub	PF00928	159 - 426	1.1e-95	Adaptor complexes medium subunit family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004476)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10730 AT1G60780
fragaria_vesca	FvH4_4g25870 FvH4_4g25870 FvH4_5g05940 FvH4_5g05940
malus_domestica	MD06G1169300.v1.1 MD13G1097400.v1.1 MD14G1174600.v1.1 MD16G1098500.v1.1
prunus_persica	Prupe.1G248000_v2.0.a1 Prupe.1G248000_v2.0.a1 Prupe.5G168100_v2.0.a1
pyrus_communis	pycom06g15080 pycom14g14570 pycom16g08380
rosa_chinensis	RchiOBHm_Chr4g0433301 RchiOBHm_Chr7g0192901
rosa_laevigata	RLG00000004314 RLG00000006765
rosa_multiflora	Rmu_sc0000354.1_g000005 Rmu_sc0002040.1_g000054
rosa_roxburghii	Rroxscaffold_3G00262560 Rroxscaffold_5G00374860
rosa_rugosa	Rorug04G0267900 Rorug07G0005500
rosa_samantha	Rh4BG329900 Rh4CG345300 Rh4DG326600 Rh7AG131200
rosa_wichuraiana	Rw4G027900 Rw7G011190

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	170, 329
Acc65I	GGTACC	1 cut(s)	1221
AccB1I	GGYRCC	2 cut(s)	8, 1221
AccII	CGCG	2 cut(s)	63, 267
AccIII	TCCGGA	2 cut(s)	984, 1178
AciI	CCGC	7 cut(s)	12, 137, 265, 377, 960, 1107, 1118
AclWI	GGATC	4 cut(s)	128, 143, 924, 1006
AcoI	YGGCCR	3 cut(s)	3, 459, 927
AcsI	RAATTY	3 cut(s)	407, 884, 1182
AcuI	CTGAAG	2 cut(s)	408, 1131
AcyI	GRCGYC	1 cut(s)	9
AfaI	GTAC	5 cut(s)	161, 341, 503, 820, 1223
AfiI	CCNNNNNNNGG	2 cut(s)	785, 913
AflIII	ACRYGT	2 cut(s)	180, 1071
AgsI	TTSAA	3 cut(s)	296, 860, 884
AhdI	GACNNNNNGTC	1 cut(s)	447
AjnI	CCWGG	2 cut(s)	1054, 1224
AluBI	AGCT	5 cut(s)	177, 246, 301, 640, 1136
AluI	AGCT	5 cut(s)	177, 246, 301, 640, 1136
Alw21I	GWGCWC	1 cut(s)	1138
Alw26I	GTCTC	4 cut(s)	315, 747, 1180, 1270
AlwI	GGATC	4 cut(s)	128, 143, 924, 1006
Ama87I	CYCGRG	1 cut(s)	629
Aor13HI	TCCGGA	2 cut(s)	984, 1178
AoxI	GGCC	5 cut(s)	3, 93, 459, 594, 927
ApaI	GGGCCC	1 cut(s)	598
ApeKI	GCWGC	6 cut(s)	86, 239, 250, 661, 921, 1004
ApoI	RAATTY	3 cut(s)	407, 884, 1182
ArsI	GACNNNNNNTTYG	2 cut(s)	461, 493
Asp700I	GAANNNNTTC	1 cut(s)	1119
Asp718I	GGTACC	1 cut(s)	1221
AspLEI	GCGC	5 cut(s)	11, 22, 63, 486, 673
AspS9I	GGNCC	3 cut(s)	594, 595, 1189
AsuHPI	GGTGA	5 cut(s)	100, 451, 838, 1159, 1252
AsuNHI	GCTAGC	1 cut(s)	242
AvaI	CYCGRG	1 cut(s)	629
AvaII	GGWCC	1 cut(s)	1189
BaeGI	GKGCMC	2 cut(s)	598, 960
BanI	GGYRCC	2 cut(s)	8, 1221
BanII	GRGCYC	2 cut(s)	598, 1138
Bbv12I	GWGCWC	1 cut(s)	1138
BbvI	GCAGC	6 cut(s)	73, 226, 237, 648, 933, 991
BccI	CCATC	3 cut(s)	427, 732, 779
BceAI	ACGGC	2 cut(s)	446, 942
BcgI	CGANNNNNNTGC	2 cut(s)	65, 99
BciT130I	CCWGG	2 cut(s)	1056, 1226
BciVI	GTATCC	1 cut(s)	489
BcoDI	GTCTC	4 cut(s)	315, 747, 1180, 1270
BfaI	CTAG	5 cut(s)	243, 302, 665, 870, 1098
BfoI	RGCGCY	2 cut(s)	12, 23
BfuI	GTATCC	1 cut(s)	489
BglI	GCCNNNNNGGC	1 cut(s)	17
BisI	GCNGC	7 cut(s)	12, 87, 240, 251, 662, 922, 1005
BlsI	GCNGC	7 cut(s)	13, 88, 241, 252, 663, 923, 1006
BmcAI	AGTACT	1 cut(s)	820
Bme1390I	CCNGG	2 cut(s)	1056, 1226
Bme18I	GGWCC	1 cut(s)	1189
BmeRI	GACNNNNNGTC	1 cut(s)	447
BmeT110I	CYCGRG	1 cut(s)	629
BmgT120I	GGNCC	3 cut(s)	594, 595, 1189
BmiI	GGNNCC	6 cut(s)	10, 595, 596, 905, 1122, 1223
BmrFI	CCNGG	2 cut(s)	1056, 1226
BmsI	GCATC	4 cut(s)	412, 570, 955, 1015
BmtI	GCTAGC	1 cut(s)	246
Bpu10I	CCTNAGC	1 cut(s)	90
Bsa29I	ATCGAT	1 cut(s)	273
BsaHI	GRCGYC	1 cut(s)	9
BsaJI	CCNNGG	4 cut(s)	15, 70, 456, 1235
BsaWI	WCCGGW	3 cut(s)	759, 984, 1178
Bsc4I	CCNNNNNNNGG	2 cut(s)	785, 913
Bse118I	RCCGGY	1 cut(s)	5
Bse1I	ACTGG	2 cut(s)	152, 907
Bse3DI	GCAATG	1 cut(s)	241
BseAI	TCCGGA	2 cut(s)	984, 1178
BseBI	CCWGG	2 cut(s)	1056, 1226
BseCI	ATCGAT	1 cut(s)	273
BseDI	CCNNGG	4 cut(s)	15, 70, 456, 1235
BseGI	GGATG	4 cut(s)	438, 532, 715, 774
BseLI	CCNNNNNNNGG	2 cut(s)	785, 913
BseMI	GCAATG	1 cut(s)	241
BseMII	CTCAG	4 cut(s)	104, 829, 836, 1265
BseNI	ACTGG	2 cut(s)	152, 907
BseSI	GKGCMC	2 cut(s)	598, 960
BseXI	GCAGC	6 cut(s)	73, 226, 237, 648, 933, 991
BsgI	GTGCAG	1 cut(s)	245
Bsh1236I	CGCG	2 cut(s)	63, 267
BshFI	GGCC	5 cut(s)	5, 95, 461, 596, 929
BshNI	GGYRCC	2 cut(s)	8, 1221
BshVI	ATCGAT	1 cut(s)	273
BsiHKAI	GWGCWC	1 cut(s)	1138
BsiHKCI	CYCGRG	1 cut(s)	629
BsiSI	CCGG	4 cut(s)	6, 760, 985, 1179
BslFI	GGGAC	1 cut(s)	65
BslI	CCNNNNNNNGG	2 cut(s)	785, 913
BsmAI	GTCTC	4 cut(s)	315, 747, 1180, 1270
BsmFI	GGGAC	1 cut(s)	65
BsnI	GGCC	5 cut(s)	5, 95, 461, 596, 929
BsoBI	CYCGRG	1 cut(s)	629
Bsp120I	GGGCCC	1 cut(s)	594
Bsp1286I	GDGCHC	3 cut(s)	598, 960, 1138
Bsp13I	TCCGGA	2 cut(s)	984, 1178
Bsp143I	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
Bsp19I	CCATGG	2 cut(s)	456, 1235
BspACI	CCGC	7 cut(s)	12, 137, 265, 377, 960, 1107, 1118
BspANI	GGCC	5 cut(s)	5, 95, 461, 596, 929
BspCNI	CTCAG	4 cut(s)	103, 828, 835, 1266
BspDI	ATCGAT	1 cut(s)	273
BspEI	TCCGGA	2 cut(s)	984, 1178
BspFNI	CGCG	2 cut(s)	63, 267
BspLI	GGNNCC	6 cut(s)	10, 595, 596, 905, 1122, 1223
BspOI	GCTAGC	1 cut(s)	246
BspPI	GGATC	4 cut(s)	128, 143, 924, 1006
BspQI	GCTCTTC	3 cut(s)	291, 1126, 1236
BspT107I	GGYRCC	2 cut(s)	8, 1221
BsrDI	GCAATG	1 cut(s)	241
BsrFI	RCCGGY	1 cut(s)	5
BsrI	ACTGG	2 cut(s)	152, 907
BssAI	RCCGGY	1 cut(s)	5
BssECI	CCNNGG	4 cut(s)	15, 70, 456, 1235
BssMI	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
BssNI	GRCGYC	1 cut(s)	9
BssT1I	CCWWGG	2 cut(s)	456, 1235
Bst2UI	CCWGG	2 cut(s)	1056, 1226
Bst4CI	ACNGT	6 cut(s)	71, 501, 579, 892, 1164, 1174
Bst6I	CTCTTC	4 cut(s)	291, 300, 1126, 1236
BstACI	GRCGYC	1 cut(s)	9
BstC8I	GCNNGC	5 cut(s)	7, 244, 248, 960, 1261
BstDEI	CTNAG	6 cut(s)	90, 317, 401, 815, 822, 1274
BstDSI	CCRYGG	3 cut(s)	70, 456, 1235
BstF5I	GGATG	4 cut(s)	438, 532, 715, 774
BstFNI	CGCG	2 cut(s)	63, 267
BstH2I	RGCGCY	2 cut(s)	12, 23
BstHHI	GCGC	5 cut(s)	11, 22, 63, 486, 673
BstKTI	GATC	7 cut(s)	136, 151, 706, 799, 919, 1001, 1082
BstMAI	GTCTC	4 cut(s)	315, 747, 1180, 1270
BstMBI	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 17, 28, 92, 259, 670, 1010, 1115
BstNI	CCWGG	2 cut(s)	1056, 1226
BstNSI	RCATGY	2 cut(s)	184, 1075
BstSCI	CCNGG	2 cut(s)	1054, 1224
BstSLI	GKGCMC	2 cut(s)	598, 960
BstUI	CGCG	2 cut(s)	63, 267
BstV1I	GCAGC	6 cut(s)	73, 226, 237, 648, 933, 991
BstX2I	RGATCY	2 cut(s)	148, 998
BstXI	CCANNNNNNTGG	1 cut(s)	706
BstYI	RGATCY	2 cut(s)	148, 998
Bsu15I	ATCGAT	1 cut(s)	273
BsuI	GTATCC	1 cut(s)	489
BsuRI	GGCC	5 cut(s)	5, 95, 461, 596, 929
BsuTUI	ATCGAT	1 cut(s)	273
BtgI	CCRYGG	3 cut(s)	70, 456, 1235
BtgZI	GCGATG	1 cut(s)	90
BtsCI	GGATG	4 cut(s)	438, 532, 715, 774
BtsI	GCAGTG	2 cut(s)	480, 493
BtsIMutI	CAGTG	5 cut(s)	159, 384, 480, 493, 734
Cac8I	GCNNGC	5 cut(s)	7, 244, 248, 960, 1261
CfoI	GCGC	5 cut(s)	11, 22, 63, 486, 673
Cfr10I	RCCGGY	1 cut(s)	5
Cfr13I	GGNCC	3 cut(s)	594, 595, 1189
ClaI	ATCGAT	1 cut(s)	273
CseI	GACGC	1 cut(s)	207
Csp6I	GTAC	5 cut(s)	160, 340, 502, 819, 1222
CviAII	CATG	4 cut(s)	181, 457, 1072, 1236
CviQI	GTAC	5 cut(s)	160, 340, 502, 819, 1222
DdeI	CTNAG	6 cut(s)	90, 317, 401, 815, 822, 1274
DinI	GGCGCC	1 cut(s)	10
DpnI	GATC	7 cut(s)	135, 150, 705, 798, 918, 1000, 1081
DpnII	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
DrdI	GACNNNNNNGTC	2 cut(s)	170, 329
DriI	GACNNNNNGTC	1 cut(s)	447
DseDI	GACNNNNNNGTC	2 cut(s)	170, 329
EaeI	YGGCCR	3 cut(s)	3, 459, 927
Eam1104I	CTCTTC	4 cut(s)	291, 300, 1126, 1236
Eam1105I	GACNNNNNGTC	1 cut(s)	447
EarI	CTCTTC	4 cut(s)	291, 300, 1126, 1236
Ecl136II	GAGCTC	1 cut(s)	1136
Eco130I	CCWWGG	2 cut(s)	456, 1235
Eco24I	GRGCYC	2 cut(s)	598, 1138
Eco47I	GGWCC	1 cut(s)	1189
Eco53kI	GAGCTC	1 cut(s)	1136
Eco57I	CTGAAG	2 cut(s)	408, 1131
Eco88I	CYCGRG	1 cut(s)	629
EcoICRI	GAGCTC	1 cut(s)	1136
EcoO109I	RGGNCCY	2 cut(s)	594, 595
EcoRI	GAATTC	2 cut(s)	884, 1182
EcoRII	CCWGG	2 cut(s)	1054, 1224
EcoT14I	CCWWGG	2 cut(s)	456, 1235
EcoT38I	GRGCYC	2 cut(s)	598, 1138
EgeI	GGCGCC	1 cut(s)	10
EheI	GGCGCC	1 cut(s)	10
ErhI	CCWWGG	2 cut(s)	456, 1235
FaeI	CATG	4 cut(s)	184, 460, 1075, 1239
FalI	AAGNNNNNCTT	2 cut(s)	1012, 1044
FaqI	GGGAC	1 cut(s)	65
FatI	CATG	4 cut(s)	180, 456, 1071, 1235
FauI	CCCGC	2 cut(s)	384, 967
FauNDI	CATATG	1 cut(s)	1009
Fnu4HI	GCNGC	7 cut(s)	12, 87, 240, 251, 662, 922, 1005
FokI	GGATG	4 cut(s)	445, 539, 722, 761
FriOI	GRGCYC	2 cut(s)	598, 1138
Fsp4HI	GCNGC	7 cut(s)	12, 87, 240, 251, 662, 922, 1005
FspBI	CTAG	5 cut(s)	243, 302, 665, 870, 1098
GlaI	GCGC	5 cut(s)	10, 21, 62, 485, 672
GluI	GCNGC	7 cut(s)	12, 87, 240, 251, 662, 922, 1005
HaeII	RGCGCY	2 cut(s)	12, 23
HaeIII	GGCC	5 cut(s)	5, 95, 461, 596, 929
HapII	CCGG	4 cut(s)	6, 760, 985, 1179
HgaI	GACGC	1 cut(s)	207
HhaI	GCGC	5 cut(s)	11, 22, 63, 486, 673
Hin1I	GRCGYC	1 cut(s)	9
Hin1II	CATG	4 cut(s)	184, 460, 1075, 1239
Hin6I	GCGC	5 cut(s)	9, 20, 61, 484, 671
HinP1I	GCGC	5 cut(s)	9, 20, 61, 484, 671
HincII	GTYRAC	2 cut(s)	544, 556
HindII	GTYRAC	2 cut(s)	544, 556
HindIII	AAGCTT	1 cut(s)	638
HinfI	GANTC	4 cut(s)	171, 311, 397, 813
HpaII	CCGG	4 cut(s)	6, 760, 985, 1179
HphI	GGTGA	5 cut(s)	100, 451, 838, 1159, 1252
Hpy166II	GTNNAC	5 cut(s)	160, 340, 544, 556, 1089
Hpy188I	TCNGA	4 cut(s)	583, 801, 1084, 1193
Hpy188III	TCNNGA	9 cut(s)	415, 707, 744, 870, 985, 1016, 1036, 1124, 1179
Hpy8I	GTNNAC	5 cut(s)	160, 340, 544, 556, 1089
Hpy99I	CGWCG	1 cut(s)	223
HpyAV	CCTTC	1 cut(s)	485
HpyCH4III	ACNGT	6 cut(s)	71, 501, 579, 892, 1164, 1174
HpyCH4IV	ACGT	2 cut(s)	448, 1283
HpyCH4V	TGCA	4 cut(s)	234, 262, 473, 1007
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 17, 28, 92, 259, 670, 1010, 1115
HpyF3I	CTNAG	6 cut(s)	90, 317, 401, 815, 822, 1274
HpySE526I	ACGT	2 cut(s)	448, 1283
Hsp92I	GRCGYC	1 cut(s)	9
Hsp92II	CATG	4 cut(s)	184, 460, 1075, 1239
HspAI	GCGC	5 cut(s)	9, 20, 61, 484, 671
KasI	GGCGCC	1 cut(s)	8
Kpn2I	TCCGGA	2 cut(s)	984, 1178
KpnI	GGTACC	1 cut(s)	1225
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Kzo9I	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
LguI	GCTCTTC	3 cut(s)	291, 1126, 1236
LmnI	GCTCC	4 cut(s)	788, 903, 1018, 1141
Lsp1109I	GCAGC	6 cut(s)	73, 226, 237, 648, 933, 991
LweI	GCATC	4 cut(s)	412, 570, 955, 1015
MaeI	CTAG	5 cut(s)	243, 302, 665, 870, 1098
MaeII	ACGT	2 cut(s)	448, 1283
MaeIII	GTNAC	3 cut(s)	345, 439, 463
MalI	GATC	7 cut(s)	135, 150, 705, 798, 918, 1000, 1081
MboI	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
MboII	GAAGA	8 cut(s)	97, 308, 317, 406, 520, 1124, 1143, 1223
MflI	RGATCY	2 cut(s)	148, 998
MhlI	GDGCHC	3 cut(s)	598, 960, 1138
MluCI	AATT	7 cut(s)	357, 380, 407, 884, 942, 1041, 1182
Mly113I	GGCGCC	1 cut(s)	9
MlyI	GAGTC	2 cut(s)	180, 807
MnlI	CCTC	7 cut(s)	25, 121, 216, 301, 430, 462, 843
MroI	TCCGGA	2 cut(s)	984, 1178
MroNI	GCCGGC	1 cut(s)	5
MroXI	GAANNNNTTC	1 cut(s)	1119
MseI	TTAA	3 cut(s)	282, 717, 1044
MslI	CAYNNNNRTG	2 cut(s)	729, 1253
MspA1I	CMGCKG	2 cut(s)	962, 1109
MspI	CCGG	4 cut(s)	6, 760, 985, 1179
MspR9I	CCNGG	2 cut(s)	1056, 1226
MvaI	CCWGG	2 cut(s)	1056, 1226
MvnI	CGCG	2 cut(s)	63, 267
MwoI	GCNNNNNNNGC	8 cut(s)	11, 17, 28, 92, 259, 670, 1010, 1115
NaeI	GCCGGC	1 cut(s)	7
NarI	GGCGCC	1 cut(s)	9
NcoI	CCATGG	2 cut(s)	456, 1235
NdeI	CATATG	1 cut(s)	1009
NdeII	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
NgoMIV	GCCGGC	1 cut(s)	5
NheI	GCTAGC	1 cut(s)	242
NlaIII	CATG	4 cut(s)	184, 460, 1075, 1239
NlaIV	GGNNCC	6 cut(s)	10, 595, 596, 905, 1122, 1223
NmuCI	GTSAC	1 cut(s)	439
NspI	RCATGY	2 cut(s)	184, 1075
PciI	ACATGT	2 cut(s)	180, 1071
PciSI	GCTCTTC	3 cut(s)	291, 1126, 1236
PcsI	WCGNNNNNNNCGW	1 cut(s)	339
PdiI	GCCGGC	1 cut(s)	7
PdmI	GAANNNNTTC	1 cut(s)	1119
PfeI	GAWTC	2 cut(s)	311, 397
PkrI	GCNGC	7 cut(s)	13, 88, 241, 252, 663, 923, 1006
PleI	GAGTC	2 cut(s)	179, 807
PluTI	GGCGCC	1 cut(s)	12
PpsI	GAGTC	2 cut(s)	179, 807
PscI	ACATGT	2 cut(s)	180, 1071
Psp124BI	GAGCTC	1 cut(s)	1138
Psp6I	CCWGG	2 cut(s)	1054, 1224
PspGI	CCWGG	2 cut(s)	1054, 1224
PspN4I	GGNNCC	6 cut(s)	10, 595, 596, 905, 1122, 1223
PspOMI	GGGCCC	1 cut(s)	594
PspPI	GGNCC	3 cut(s)	594, 595, 1189
PsuI	RGATCY	2 cut(s)	148, 998
RsaI	GTAC	5 cut(s)	161, 341, 503, 820, 1223
RsaNI	GTAC	5 cut(s)	160, 340, 502, 819, 1222
RseI	CAYNNNNRTG	2 cut(s)	729, 1253
SacI	GAGCTC	1 cut(s)	1138
SapI	GCTCTTC	3 cut(s)	291, 1126, 1236
SaqAI	TTAA	3 cut(s)	282, 717, 1044
SatI	GCNGC	7 cut(s)	12, 87, 240, 251, 662, 922, 1005
Sau3AI	GATC	7 cut(s)	133, 148, 703, 796, 916, 998, 1079
Sau96I	GGNCC	3 cut(s)	594, 595, 1189
ScaI	AGTACT	1 cut(s)	820
SchI	GAGTC	2 cut(s)	180, 807
ScrFI	CCNGG	2 cut(s)	1056, 1226
SduI	GDGCHC	3 cut(s)	598, 960, 1138
SfaNI	GCATC	4 cut(s)	412, 570, 955, 1015
SfoI	GGCGCC	1 cut(s)	10
SinI	GGWCC	1 cut(s)	1189
SmiMI	CAYNNNNRTG	2 cut(s)	729, 1253
Sse9I	AATT	7 cut(s)	357, 380, 407, 884, 942, 1041, 1182
SsiI	CCGC	7 cut(s)	12, 137, 265, 377, 960, 1107, 1118
SspDI	GGCGCC	1 cut(s)	8
SspMI	CTAG	5 cut(s)	243, 302, 665, 870, 1098
SstI	GAGCTC	1 cut(s)	1138
StyD4I	CCNGG	2 cut(s)	1054, 1224
StyI	CCWWGG	2 cut(s)	456, 1235
TaaI	ACNGT	6 cut(s)	71, 501, 579, 892, 1164, 1174
TaiI	ACGT	2 cut(s)	451, 1286
TaqI	TCGA	7 cut(s)	33, 120, 221, 273, 686, 745, 939
TaqII	GACCGA	1 cut(s)	434
TasI	AATT	7 cut(s)	357, 380, 407, 884, 942, 1041, 1182
TatI	WGTACW	1 cut(s)	818
TauI	GCSGC	1 cut(s)	14
TfiI	GAWTC	2 cut(s)	311, 397
Tru1I	TTAA	3 cut(s)	282, 717, 1044
Tru9I	TTAA	3 cut(s)	282, 717, 1044
TscAI	CASTG	5 cut(s)	159, 391, 480, 493, 734
TseFI	GTSAC	1 cut(s)	439
TseI	GCWGC	6 cut(s)	86, 239, 250, 661, 921, 1004
Tsp45I	GTSAC	1 cut(s)	439
TspDTI	ATGAA	4 cut(s)	106, 175, 369, 763
TspRI	CASTG	5 cut(s)	159, 391, 480, 493, 734
VpaK11BI	GGWCC	1 cut(s)	1189
XapI	RAATTY	3 cut(s)	407, 884, 1182
XbaI	TCTAGA	1 cut(s)	869
XceI	RCATGY	2 cut(s)	184, 1075
XmnI	GAANNNNTTC	1 cut(s)	1119
XspI	CTAG	5 cut(s)	243, 302, 665, 870, 1098
ZrmI	AGTACT	1 cut(s)	820

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.