MD06G1174400.v1.1

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014735
Physical Location & Seq
Forward (+)
31451830 .. 31455454
3625 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1174400.v1.1.491

Sequence Viewer

Length: 2541 bp
ATGGCGTTTGTAGTGCCACAAGGCTGGTATTCAATTCATTACCCGGCAATACAATCGGAGGCGGCGTCACACTACAAATTCCGACGTCTAAGTACGCCGTGCTTTCTATCTTTTACTTCAAAGTTTCATAATTTCTGCTCCGGAATCCAATTCCTGAAAGCCCCATCTCGGAAACTGATTCTGGGACGTGGGTTTTCGCGGGAGAGTTCGCTGCTCGAAGTGTCGTCGGCCGGCCAGCTGATTAAGGACTACGTGGAGGACGGGAACTTCGAAGATGCAATCACCATTTATGTCAAAGTTCTTGAATTTGGTCTGCCGGTTTCGGAATTTCGATTCTTTCCCTGTTTGATTAAGGCGTTCGGGGGGCTTTGTGATGCCGGAAAGGTTCGCGAGATTCATGGGCACGTTTTGAAATTAGGAGTTTTGGATGATGTTTACGATGTCAATTCGCTTTTGGGTGTTTACTGGAAGTGCTGGGCTGTTGAGGATGCAATCCAGCTGTTTGAGAAAATGCGTGAGAGAGATTTGGTTTCTTGGAATACTATGATATCTGGGCTTTGCCATTGGGGAGATTATATGGGTTCGTTGAGGATGTTTAGTCGGATGGTTAATGATTACTGGGTGTTGCCAAACCGGGTGGCTTGCCTTTCTCTTCGGTTCTCCTCAGTTCAGTGTTCAGTTCATGGAAGGGAAATTCATGGGTTTGTTGTGAAAAGGGAGATAGCTACAGATCAGTTCTTGGTTAGTGGATTAATCGATATGTACATGAAATGTGGGGATGTAAAGAATGCGGAATATGTTTTTGGGAGCATTGTTTATGAAGAATCGATCAGAGGGAATCCGGTGATATGGAATGTGATGATCTCGGGCTATGTTGCGAACGGGCGTTTGCCACAAGCAGTGGAGTTGTTCCTAGAGATGTTAGAAATTGGTTTATCACCAGATACTTCCACAATGGTCGCTGTTATAGGTTTGTGCTCTCAGCTGTTGGATTTAGCATTGGGAAGGCAAATCCATAAGTTCTGTTATAGCATTCAATTGAACCATGATGCAAGAGTTGAAACGGCTCTTATGGACATGTATTTTAAATGCGGTGATAGCAAAGCTGGTCTTGAAATCTTTCAAAGATCTCAAAATCGTAACATTGTCATGTGGGGAGCTGTCATCTCAAATTTCGCTCAAAGCAGTCATCCTCATGAGGCATTGAATCTGTTCCATAACTATGTGTTAGAATATGGCTTTGTGGATTCTGTCATCGTATTGGCTGTTCTGCGAGCCTGTTCTTCCTTGGCTGTTAGGCCTAGAGGCATGGAAATCCATGGGCTAGTAGTCAAAATGGGGCTTGATTCAGATGTTTTTGTCGGAGGTACCTTAGTCGATATGTATGCGAAATGCAGAGACATGGAGTCAGCTCAAATTGTCTTTTATAGACTACCTGCTAGAGATTTGGTATCTTGGAATGCCTTGATATCTGGATATACTCAGAATGAGTTTCCAGATGAAGCTTTAAAGGCATTCCTCGACATGCAGTCCGATGGAGTCAAACCCAATGCCGTGACAGTTGCGAGTATGCTGTCAGTTTGTACTCAGTTGTCAGTCATGAAGTCGTGCAAGGAGGTTCACAGTTACATTTTACGACAAGAATTTGAGTCCAATGTTCTTGTGAGCAATTCCCTTATAACTACCTATGCAAAATGTGGAGACATGAAGAGCTCATGGGCTACATTTGAGAGCATGCCTGAAAGAACTGAAGTATCATGGAATTCAATTCTTTTGGGGTTAGGAATGCACGGTCATGTGGATGAAACATTCGGTTTATTTGAGAAAATGGAAGCAGCAGGAATGAAGCCTGACCATGCGACTTTTACAGCTCTACTGTCTGCTTGCAGCCATGCAGGGAGGGTTGAAGAGGGATTGAAATATTTTAAACGTATGGTCGAGGATTTCACAATTGAACCTCGACTTGAACAGTACACCTGCATGGTTGATCTTTTAGGCCGAGCTGGCCATCTAAAACATGCATACGATATTATTTTGGCAATGCCTTGCACCCCAGATGATCGTGTATGGGGATCATTGCTCGGATCATGCAAAATTCACGGTGATGAAAGACTTGCAGAAGTTGTGTCTGATCATATCTTTGAACTCGATCCCACTAGTATTGGTTACCGTACACTTCTTGCAAACTTGTATGAAGAGTATGGGAAATGGAATGATGTTACTAGGATTAGATCGGATATCAAAGGCAGGGGGCTCAAGAAGACACCTGGCTGCAGTTGGATTGAAGTCGATAGTAATGTTCATGTATTTATGGCGGGTGATCAGTCTCATAATCAGTTGGAGGAGATATATGCTACGGTTGAATGTTTAACAAATGAAATAAGGAAGGCAGGATATATTCCTCAATCACCTACAGTAAGTGTTCGGCATGATGAGGTTAATCATGAAAATCTTTCATCATTTAAAGACGATGATTTGCTGTTTCCGGTTGCAAGTAGAAACCATAGCCGAACAGGTTCAGCGGAATTGATGATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

847

Amino Acids

95.12

Weight (kDa)

5.75

Isoelectric Point (pI)

41.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 177 - 204 7.6e-06 PPR repeat
PPR_2 PF13041 281 - 326 2.5e-09 PPR repeat family
PPR PF01535 282 - 312 1.2e-07 PPR repeat
PPR_2 PF13041 482 - 528 1.2e-10 PPR repeat family
PPR PF01535 484 - 514 1.9e-07 PPR repeat
PPR_2 PF13041 583 - 630 9e-11 PPR repeat family
PPR_long PF17177 599 - 677 3.3e-06 Pentacotripeptide-repeat region of PRORP
PPR PF01535 621 - 647 9.6e-06 PPR repeat
E_motif PF20431 700 - 762 6.4e-16 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014735)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05260
malus_domestica MD06G1174400.v1.1 MD06G1174600.v1.1
prunus_persica Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1
pyrus_communis pycom06g15570
rosa_chinensis RchiOBHm_Chr7g0193611
rosa_laevigata RLG00000004256
rosa_multiflora Rmu_sc0042944.1_g000002
rosa_roxburghii Rroxscaffold_3G00261960
rosa_rugosa Rorug07G0011000 Rorug07G0011100
rosa_samantha Rh7AG137600 Rh7BG137800 Rh7CG141400
rosa_wichuraiana Rw7G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1679
AarI CACCTGC 1 cut(s) 1985
AatII GACGTC 1 cut(s) 88
Acc36I ACCTGC 2 cut(s) 1444, 1985
Acc65I GGTACC 1 cut(s) 1367
AccB1I GGYRCC 1 cut(s) 1367
AccII CGCG 2 cut(s) 199, 390
AccIII TCCGGA 1 cut(s) 140
AciI CCGC 6 cut(s) 62, 199, 791, 1092, 2315, 2522
AclWI GGATC 3 cut(s) 2080, 2092, 2144
AcoI YGGCCR 3 cut(s) 228, 232, 2005
AcsI RAATTY 8 cut(s) 77, 305, 326, 693, 1171, 1643, 1762, 2094
AcuI CTGAAG 1 cut(s) 1770
AcyI GRCGYC 2 cut(s) 65, 85
AfaI GTAC 6 cut(s) 94, 764, 1369, 1585, 1973, 2173
AfiI CCNNNNNNNGG 1 cut(s) 168
AflIII ACRYGT 1 cut(s) 1077
AhdI GACNNNNNGTC 2 cut(s) 1405, 1528
AhlI ACTAGT 1 cut(s) 2156
AjiI CACGTC 1 cut(s) 188
AjnI CCWGG 1 cut(s) 2266
AjuI GAANNNNNNNTTGG 3 cut(s) 786, 818, 2517
Alw21I GWGCWC 2 cut(s) 980, 1715
Alw26I GTCTC 3 cut(s) 1392, 1695, 2331
AlwI GGATC 3 cut(s) 2080, 2092, 2144
Ama87I CYCGRG 1 cut(s) 865
Aor13HI TCCGGA 1 cut(s) 140
AoxI GGCC 5 cut(s) 228, 232, 1298, 1996, 2005
ApeKI GCWGC 4 cut(s) 211, 1835, 1887, 2271
ApoI RAATTY 8 cut(s) 77, 305, 326, 693, 1171, 1643, 1762, 2094
ArsI GACNNNNNNTTYG 2 cut(s) 251, 283
AseI ATTAAT 1 cut(s) 752
Asp700I GAANNNNTTC 3 cut(s) 1119, 1211, 2515
Asp718I GGTACC 1 cut(s) 1367
AsuC2I CCSGG 2 cut(s) 44, 635
AsuHPI GGTGA 7 cut(s) 274, 856, 930, 1106, 2114, 2330, 2400
AsuII TTCGAA 1 cut(s) 270
AvaI CYCGRG 1 cut(s) 865
BaeGI GKGCMC 1 cut(s) 405
BaeI ACNNNNGTAYC 4 cut(s) 1359, 1392, 1737, 1770
BalI TGGCCA 1 cut(s) 2007
BanI GGYRCC 1 cut(s) 1367
BanII GRGCYC 2 cut(s) 1715, 2256
BbsI GAAGAC 1 cut(s) 2267
Bbv12I GWGCWC 2 cut(s) 980, 1715
BbvI GCAGC 4 cut(s) 198, 1847, 1899, 2258
BccI CCATC 4 cut(s) 172, 598, 1529, 2016
BceAI ACGGC 3 cut(s) 82, 1080, 1538
BcgI CGANNNNNNTGC 4 cut(s) 36, 70, 1367, 1401
BciT130I CCWGG 1 cut(s) 2268
BclI TGATCA 2 cut(s) 2131, 2320
BcnI CCSGG 2 cut(s) 44, 635
BcoDI GTCTC 3 cut(s) 1392, 1695, 2331
BcuI ACTAGT 1 cut(s) 2156
BfaI CTAG 6 cut(s) 914, 1302, 1325, 1440, 2157, 2223
BfmI CTRYAG 3 cut(s) 726, 2272, 2412
BfuAI ACCTGC 2 cut(s) 1444, 1985
BglI GCCNNNNNGGC 1 cut(s) 2004
BglII AGATCT 1 cut(s) 1127
BisI GCNGC 5 cut(s) 63, 212, 1836, 1888, 2272
BlsI GCNGC 5 cut(s) 64, 213, 1837, 1889, 2273
Bme1390I CCNGG 3 cut(s) 44, 635, 2268
BmeRI GACNNNNNGTC 2 cut(s) 1405, 1528
BmeT110I CYCGRG 1 cut(s) 865
BmgBI CACGTC 1 cut(s) 188
BmiI GGNNCC 1 cut(s) 1369
BmrFI CCNGG 3 cut(s) 44, 635, 2268
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 4 cut(s) 265, 364, 478, 1039
BmuI ACTGGG 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 2267
Bpu14I TTCGAA 1 cut(s) 270
BpuEI CTTGAG 1 cut(s) 2240
BpuMI CCSGG 2 cut(s) 44, 635
Bsa29I ATCGAT 2 cut(s) 756, 827
BsaAI YACGTR 1 cut(s) 253
BsaHI GRCGYC 2 cut(s) 65, 85
BsaJI CCNNGG 2 cut(s) 1287, 1318
BsaWI WCCGGW 3 cut(s) 140, 841, 2485
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 1 cut(s) 168
Bse118I RCCGGY 2 cut(s) 230, 316
Bse1I ACTGG 2 cut(s) 470, 623
Bse3DI GCAATG 2 cut(s) 2046, 2075
BseAI TCCGGA 1 cut(s) 140
BseBI CCWGG 1 cut(s) 2268
BseCI ATCGAT 2 cut(s) 756, 827
BseDI CCNNGG 2 cut(s) 1287, 1318
BseGI GGATG 7 cut(s) 433, 493, 597, 609, 784, 1189, 1807
BseLI CCNNNNNNNGG 1 cut(s) 168
BseMI GCAATG 2 cut(s) 2046, 2075
BseMII CTCAG 4 cut(s) 678, 995, 1496, 1601
BseNI ACTGG 2 cut(s) 470, 623
BseRI GAGGAG 2 cut(s) 652, 2357
BseSI GKGCMC 1 cut(s) 405
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 4 cut(s) 198, 1847, 1899, 2258
BseYI CCCAGC 1 cut(s) 474
Bsh1236I CGCG 2 cut(s) 199, 390
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 5 cut(s) 230, 234, 1300, 1998, 2007
BshNI GGYRCC 1 cut(s) 1367
BshVI ATCGAT 2 cut(s) 756, 827
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 2 cut(s) 980, 1715
BsiHKCI CYCGRG 1 cut(s) 865
BsiSI CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 842, 2486
BslFI GGGAC 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 168
BsmAI GTCTC 3 cut(s) 1392, 1695, 2331
BsmFI GGGAC 1 cut(s) 198
BsmI GAATGC 5 cut(s) 793, 1032, 1465, 1514, 1791
BsnI GGCC 5 cut(s) 230, 234, 1300, 1998, 2007
BsoBI CYCGRG 1 cut(s) 865
Bsp119I TTCGAA 1 cut(s) 270
Bsp1286I GDGCHC 4 cut(s) 405, 980, 1715, 2256
Bsp13I TCCGGA 1 cut(s) 140
Bsp1407I TGTACA 1 cut(s) 762
Bsp19I CCATGG 1 cut(s) 1318
Bsp68I TCGCGA 1 cut(s) 390
BspACI CCGC 6 cut(s) 62, 199, 791, 1092, 2315, 2522
BspANI GGCC 5 cut(s) 230, 234, 1300, 1998, 2007
BspCNI CTCAG 4 cut(s) 677, 994, 1495, 1600
BspDI ATCGAT 2 cut(s) 756, 827
BspEI TCCGGA 1 cut(s) 140
BspFNI CGCG 2 cut(s) 199, 390
BspHI TCATGA 3 cut(s) 1195, 1599, 2443
BspLI GGNNCC 1 cut(s) 1369
BspMAI CTGCAG 1 cut(s) 2276
BspMI ACCTGC 2 cut(s) 1444, 1985
BspPI GGATC 3 cut(s) 2080, 2092, 2144
BspQI GCTCTTC 1 cut(s) 1703
BspT104I TTCGAA 1 cut(s) 270
BspT107I GGYRCC 1 cut(s) 1367
BsrDI GCAATG 2 cut(s) 2046, 2075
BsrFI RCCGGY 2 cut(s) 230, 316
BsrGI TGTACA 1 cut(s) 762
BsrI ACTGG 2 cut(s) 470, 623
BssAI RCCGGY 2 cut(s) 230, 316
BssECI CCNNGG 2 cut(s) 1287, 1318
BssNI GRCGYC 2 cut(s) 65, 85
BssT1I CCWWGG 2 cut(s) 1287, 1318
Bst2UI CCWGG 1 cut(s) 2268
Bst4CI ACNGT 9 cut(s) 1561, 1625, 1793, 1878, 1971, 2102, 2171, 2359, 2416
Bst6I CTCTTC 4 cut(s) 657, 1703, 1902, 2190
BstACI GRCGYC 2 cut(s) 65, 85
BstAUI TGTACA 1 cut(s) 762
BstBAI YACGTR 1 cut(s) 253
BstBI TTCGAA 1 cut(s) 270
BstC8I GCNNGC 7 cut(s) 232, 236, 643, 1275, 1736, 1885, 2005
BstDEI CTNAG 6 cut(s) 89, 664, 981, 1372, 1482, 1587
BstDSI CCRYGG 1 cut(s) 1318
BstEII GGTNACC 1 cut(s) 2165
BstF5I GGATG 7 cut(s) 433, 493, 597, 609, 784, 1189, 1807
BstFNI CGCG 2 cut(s) 199, 390
BstMAI GTCTC 3 cut(s) 1392, 1695, 2331
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 3 cut(s) 1098, 1511, 2004
BstNI CCWGG 1 cut(s) 2268
BstNSI RCATGY 4 cut(s) 1081, 1528, 1738, 2021
BstPI GGTNACC 1 cut(s) 2165
BstSCI CCNGG 3 cut(s) 42, 633, 2266
BstSFI CTRYAG 3 cut(s) 726, 2272, 2412
BstSLI GKGCMC 1 cut(s) 405
BstUI CGCG 2 cut(s) 199, 390
BstV1I GCAGC 4 cut(s) 198, 1847, 1899, 2258
BstV2I GAAGAC 1 cut(s) 2267
BstX2I RGATCY 1 cut(s) 1127
BstXI CCANNNNNNTGG 1 cut(s) 24
BstYI RGATCY 1 cut(s) 1127
BstZI CGGCCG 1 cut(s) 228
Bsu15I ATCGAT 2 cut(s) 756, 827
BsuRI GGCC 5 cut(s) 230, 234, 1300, 1998, 2007
BsuTUI ATCGAT 2 cut(s) 756, 827
BtgI CCRYGG 1 cut(s) 1318
BtrI CACGTC 1 cut(s) 188
BtsCI GGATG 7 cut(s) 433, 493, 597, 609, 784, 1189, 1807
BtsI GCAGTG 1 cut(s) 906
BtsIMutI CAGTG 2 cut(s) 677, 906
BtuMI TCGCGA 1 cut(s) 390
BveI ACCTGC 2 cut(s) 1444, 1985
Cac8I GCNNGC 7 cut(s) 232, 236, 643, 1275, 1736, 1885, 2005
CciI TCATGA 3 cut(s) 1195, 1599, 2443
Cfr10I RCCGGY 2 cut(s) 230, 316
ClaI ATCGAT 2 cut(s) 756, 827
CseI GACGC 1 cut(s) 54
Csp6I GTAC 6 cut(s) 93, 763, 1368, 1584, 1972, 2172
CspCI CAANNNNNGTGG 4 cut(s) 618, 653, 2143, 2178
CviQI GTAC 6 cut(s) 93, 763, 1368, 1584, 1972, 2172
DdeI CTNAG 6 cut(s) 89, 664, 981, 1372, 1482, 1587
DraI TTTAAA 4 cut(s) 1087, 1509, 1927, 2464
DriI GACNNNNNGTC 2 cut(s) 1405, 1528
EaeI YGGCCR 3 cut(s) 228, 232, 2005
EagI CGGCCG 1 cut(s) 228
Eam1104I CTCTTC 4 cut(s) 657, 1703, 1902, 2190
Eam1105I GACNNNNNGTC 2 cut(s) 1405, 1528
EarI CTCTTC 4 cut(s) 657, 1703, 1902, 2190
Ecl136II GAGCTC 1 cut(s) 1713
EclXI CGGCCG 1 cut(s) 228
Eco130I CCWWGG 2 cut(s) 1287, 1318
Eco147I AGGCCT 1 cut(s) 1300
Eco24I GRGCYC 2 cut(s) 1715, 2256
Eco32I GATATC 3 cut(s) 549, 1470, 2239
Eco52I CGGCCG 1 cut(s) 228
Eco53kI GAGCTC 1 cut(s) 1713
Eco57I CTGAAG 1 cut(s) 1770
Eco88I CYCGRG 1 cut(s) 865
Eco91I GGTNACC 1 cut(s) 2165
EcoICRI GAGCTC 1 cut(s) 1713
EcoO65I GGTNACC 1 cut(s) 2165
EcoRI GAATTC 1 cut(s) 1762
EcoRII CCWGG 1 cut(s) 2266
EcoRV GATATC 3 cut(s) 549, 1470, 2239
EcoT14I CCWWGG 2 cut(s) 1287, 1318
EcoT22I ATGCAT 1 cut(s) 2023
EcoT38I GRGCYC 2 cut(s) 1715, 2256
ErhI CCWWGG 2 cut(s) 1287, 1318
FalI AAGNNNNNCTT 2 cut(s) 1095, 1127
FaqI GGGAC 1 cut(s) 198
FauI CCCGC 2 cut(s) 192, 2308
FbaI TGATCA 2 cut(s) 2131, 2320
Fnu4HI GCNGC 5 cut(s) 63, 212, 1836, 1888, 2272
FokI GGATG 7 cut(s) 440, 500, 604, 616, 791, 1176, 1814
FriOI GRGCYC 2 cut(s) 1715, 2256
FseI GGCCGGCC 1 cut(s) 234
Fsp4HI GCNGC 5 cut(s) 63, 212, 1836, 1888, 2272
FspBI CTAG 6 cut(s) 914, 1302, 1325, 1440, 2157, 2223
GluI GCNGC 5 cut(s) 63, 212, 1836, 1888, 2272
GsaI CCCAGC 1 cut(s) 478
HaeIII GGCC 5 cut(s) 230, 234, 1300, 1998, 2007
HapII CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 842, 2486
HgaI GACGC 1 cut(s) 54
Hin1I GRCGYC 2 cut(s) 65, 85
HindIII AAGCTT 1 cut(s) 1503
HpaII CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 842, 2486
HphI GGTGA 7 cut(s) 274, 856, 930, 1106, 2114, 2330, 2400
Hpy166II GTNNAC 5 cut(s) 436, 463, 1621, 1974, 2174
Hpy8I GTNNAC 5 cut(s) 436, 463, 1621, 1974, 2174
Hpy99I CGWCG 2 cut(s) 87, 229
HpyAV CCTTC 3 cut(s) 681, 999, 2380
HpyCH4III ACNGT 9 cut(s) 1561, 1625, 1793, 1878, 1971, 2102, 2171, 2359, 2416
HpyCH4IV ACGT 5 cut(s) 85, 187, 252, 405, 1930
HpyF10VI GCNNNNNNNGC 3 cut(s) 1098, 1511, 2004
HpyF3I CTNAG 6 cut(s) 89, 664, 981, 1372, 1482, 1587
HpySE526I ACGT 5 cut(s) 85, 187, 252, 405, 1930
Hsp92I GRCGYC 2 cut(s) 65, 85
Kpn2I TCCGGA 1 cut(s) 140
KpnI GGTACC 1 cut(s) 1371
KroI GCCGGC 1 cut(s) 230
KroNI GCCGGC 1 cut(s) 232
Ksp22I TGATCA 2 cut(s) 2131, 2320
LguI GCTCTTC 1 cut(s) 1703
LmnI GCTCC 3 cut(s) 143, 807, 1157
Lsp1109I GCAGC 4 cut(s) 198, 1847, 1899, 2258
LweI GCATC 4 cut(s) 265, 364, 478, 1039
MaeI CTAG 6 cut(s) 914, 1302, 1325, 1440, 2157, 2223
MaeII ACGT 5 cut(s) 85, 187, 252, 405, 1930
MaeIII GTNAC 6 cut(s) 66, 1139, 1555, 1625, 2165, 2218
MboII GAAGA 8 cut(s) 284, 644, 833, 1275, 1720, 1919, 2207, 2272
MfeI CAATTG 2 cut(s) 1037, 1950
MflI RGATCY 1 cut(s) 1127
MhlI GDGCHC 4 cut(s) 405, 980, 1715, 2256
MlsI TGGCCA 1 cut(s) 2007
MluNI TGGCCA 1 cut(s) 2007
MlyI GAGTC 3 cut(s) 1415, 1548, 1658
MmeI TCCRAC 6 cut(s) 106, 581, 969, 1342, 2258, 2319
Mox20I TGGCCA 1 cut(s) 2007
Mph1103I ATGCAT 1 cut(s) 2023
MroI TCCGGA 1 cut(s) 140
MroNI GCCGGC 1 cut(s) 230
MroXI GAANNNNTTC 3 cut(s) 1119, 1211, 2515
MscI TGGCCA 1 cut(s) 2007
MslI CAYNNNNRTG 7 cut(s) 1148, 1194, 1221, 1794, 1800, 1979, 2103
Msp20I TGGCCA 1 cut(s) 2007
MspA1I CMGCKG 4 cut(s) 238, 499, 985, 2522
MspI CCGG 8 cut(s) 44, 141, 231, 317, 378, 634, 842, 2486
MspR9I CCNGG 3 cut(s) 44, 635, 2268
MunI CAATTG 2 cut(s) 1037, 1950
Mva1269I GAATGC 5 cut(s) 793, 1032, 1465, 1514, 1791
MvaI CCWGG 1 cut(s) 2268
MvnI CGCG 2 cut(s) 199, 390
MwoI GCNNNNNNNGC 3 cut(s) 1098, 1511, 2004
NaeI GCCGGC 1 cut(s) 232
NciI CCSGG 2 cut(s) 44, 635
NcoI CCATGG 1 cut(s) 1318
NgoMIV GCCGGC 1 cut(s) 230
NlaIV GGNNCC 1 cut(s) 1369
NmeAIII GCCGAG 1 cut(s) 2024
NmuCI GTSAC 2 cut(s) 66, 1555
NruI TCGCGA 1 cut(s) 390
NsiI ATGCAT 1 cut(s) 2023
NspI RCATGY 4 cut(s) 1081, 1528, 1738, 2021
NspV TTCGAA 1 cut(s) 270
PaeI GCATGC 1 cut(s) 1738
PagI TCATGA 3 cut(s) 1195, 1599, 2443
PaqCI CACCTGC 1 cut(s) 1985
PceI AGGCCT 1 cut(s) 1300
PciI ACATGT 1 cut(s) 1077
PciSI GCTCTTC 1 cut(s) 1703
PcsI WCGNNNNNNNCGW 2 cut(s) 62, 267
PctI GAATGC 5 cut(s) 793, 1032, 1465, 1514, 1791
PdiI GCCGGC 1 cut(s) 232
PdmI GAANNNNTTC 3 cut(s) 1119, 1211, 2515
PfeI GAWTC 9 cut(s) 144, 178, 333, 394, 824, 838, 1207, 1247, 1346
PkrI GCNGC 5 cut(s) 64, 213, 1837, 1889, 2273
PleI GAGTC 3 cut(s) 1414, 1547, 1657
PpsI GAGTC 3 cut(s) 1414, 1547, 1657
Ppu21I YACGTR 1 cut(s) 253
PscI ACATGT 1 cut(s) 1077
PshBI ATTAAT 1 cut(s) 752
PsiI TTATAA 1 cut(s) 1679
Psp124BI GAGCTC 1 cut(s) 1715
Psp6I CCWGG 1 cut(s) 2266
PspEI GGTNACC 1 cut(s) 2165
PspFI CCCAGC 1 cut(s) 474
PspGI CCWGG 1 cut(s) 2266
PspN4I GGNNCC 1 cut(s) 1369
PsrI GAACNNNNNNTAC 2 cut(s) 1251, 1283
PstI CTGCAG 1 cut(s) 2276
PsuI RGATCY 1 cut(s) 1127
PvuII CAGCTG 3 cut(s) 238, 499, 985
RigI GGCCGGCC 1 cut(s) 234
RruI TCGCGA 1 cut(s) 390
RsaI GTAC 6 cut(s) 94, 764, 1369, 1585, 1973, 2173
RsaNI GTAC 6 cut(s) 93, 763, 1368, 1584, 1972, 2172
RseI CAYNNNNRTG 7 cut(s) 1148, 1194, 1221, 1794, 1800, 1979, 2103
SacI GAGCTC 1 cut(s) 1715
SapI GCTCTTC 1 cut(s) 1703
SatI GCNGC 5 cut(s) 63, 212, 1836, 1888, 2272
SchI GAGTC 3 cut(s) 1415, 1548, 1658
ScrFI CCNGG 3 cut(s) 44, 635, 2268
SduI GDGCHC 4 cut(s) 405, 980, 1715, 2256
SfaNI GCATC 4 cut(s) 265, 364, 478, 1039
SfcI CTRYAG 3 cut(s) 726, 2272, 2412
SfiI GGCCNNNNNGGCC 1 cut(s) 2004
SfuI TTCGAA 1 cut(s) 270
SmiMI CAYNNNNRTG 7 cut(s) 1148, 1194, 1221, 1794, 1800, 1979, 2103
SmlI CTYRAG 1 cut(s) 2255
SmoI CTYRAG 1 cut(s) 2255
SpeI ACTAGT 1 cut(s) 2156
SphI GCATGC 1 cut(s) 1738
SseBI AGGCCT 1 cut(s) 1300
SsiI CCGC 6 cut(s) 62, 199, 791, 1092, 2315, 2522
SspI AATATT 1 cut(s) 1922
SspMI CTAG 6 cut(s) 914, 1302, 1325, 1440, 2157, 2223
SstI GAGCTC 1 cut(s) 1715
StuI AGGCCT 1 cut(s) 1300
StyD4I CCNGG 3 cut(s) 42, 633, 2266
StyI CCWWGG 2 cut(s) 1287, 1318
TaaI ACNGT 9 cut(s) 1561, 1625, 1793, 1878, 1971, 2102, 2171, 2359, 2416
TaiI ACGT 5 cut(s) 88, 190, 255, 408, 1933
TatI WGTACW 3 cut(s) 762, 1583, 1971
TauI GCSGC 1 cut(s) 65
TfiI GAWTC 9 cut(s) 144, 178, 333, 394, 824, 838, 1207, 1247, 1346
TscAI CASTG 2 cut(s) 677, 906
TseFI GTSAC 2 cut(s) 66, 1555
TseI GCWGC 4 cut(s) 211, 1835, 1887, 2271
Tsp45I GTSAC 2 cut(s) 66, 1555
TspRI CASTG 2 cut(s) 677, 906
VspI ATTAAT 1 cut(s) 752
XapI RAATTY 8 cut(s) 77, 305, 326, 693, 1171, 1643, 1762, 2094
XceI RCATGY 4 cut(s) 1081, 1528, 1738, 2021
XmnI GAANNNNTTC 3 cut(s) 1119, 1211, 2515
XspI CTAG 6 cut(s) 914, 1302, 1325, 1440, 2157, 2223
ZraI GACGTC 1 cut(s) 86
Zsp2I ATGCAT 1 cut(s) 2023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.