Rh7CG141400

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0014735
Physical Location & Seq
Forward (+)
11485350 .. 11489523
4174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG141400.1

Sequence Viewer

Length: 2535 bp
ATGGCTGCTGTGAGAGTGAGACCACAAGGCTCGTGTTCGCTAAGACCATCGGAGCCATTACACTGCTACAAGTTGAGAGGTGGTGCTACAACGACGCCTTGCTCAGTTTCTTTTGCTTCAAAGTTTCAATCTTTATGTTGCAAATCACAGTTCCTGGGAAACCCATCTCGGAAATCGAATTCCAGAGGTGGGTTTTCGAGGAATTGTTCCCGGATTGATGTTGTTTCGGCCGGTGAGCTGATCAAGAGGTACGTTGAGAATGGGAATTGCGAGGATGCGAAAGCGGTTTATGTTAAAATGCTTGAATTTGGGTTGCCTGTAGCTGAGGAATTTCAGTTCTTTCCGATTTTGATTAAGGCCTTTGGGGGGCTTTGTGATGTGGGCAAGGCTAGGGAGATTCATGGGCATGTGTTGAAATTGGGGGTTTTGGGCGATGTTTACGATGCCAATTCGATTTTGGGGGTTTATTGGAAATGTGGGGAGGTTGAGGATGCCGTTCAGATGTTTGAGAAAATGCCTGAGAGGGATTTGGTGTCTTGGAATACGATGATATCTGGGTTTTGCCATTCAGGGGATTATGTGAGCTCGTTGGGGATGTTTAGTCGGATGGTTCGGGATCATTGGGTGTTGCCTAATCGGGTGGCTTGCCTTTCGGCTCTCACTTCGTGCTCTTCGGTTCAGTCTTTGGTTCATGGCAGAGAGATTCATGGCTTTGCCGTGAAGCGTTGGCTGGATGGTGATCAGTTTATGGTTAGTGGGTTGATTGATATGTACATGAAATGTGGGGATGTGAAGAATGCGGAATGTGTTTATAGGAGCGTTATTGGTGAAGCGTGTATTAGAGAAAATACAGTGATATGGAATGTGATGATTTCGGGCTATGTCTCTAATGGGTGTTGGCCTCAGGCGGTGGAGTTGTTTCTAGAGATGTTGGCAATTGGGTTGTTACCCGATACTTCCACAATGGTGGCCATTACAGGTTTGTGCTCTCACCTAATGGATTTAGCATTCGGAAAGCAGATCCATAAATTCTGTTTGAGCACTCAATTGAACACTGATGTACGAGTTGAAACAGCTCTTATGGACATGTATTTCAAATGTGGTGATAGTACAACTGGTCTTGAAATCTTCAGAAGGTCCCTGAATCGTAACATGGTCATGTGGGGCGCCGCCATCTCAAATTTTGCTCAAGGTAGTCATCCTCGTGAGGCATTGAATCTGTTTCATGACTATATTTTAGAATATGGCTTTGTGGATTCTGTCATAATCTTGGCTGTTCTGCGGGCTTGTTCATCCCTGGCTGTTAAGTCTATAGGGATGGAAATTCATGGGCTACTAGTGAAATTGGGGTTTGATTCAGACCATTTTGTTGCCAGTGCATTAGTTGATTTTTATTCAAAAACCAGAGATATTGAGTCTGCTCAAAAAGTATTTCACAGACTACCAGCTAGAGATTTGGTGTCATGGAATGCCTTGATATCCGGATATACTCAGAATGAGTATCTAGATGAAGCTTTAAAGGCATTCCTTGACATGCAACTTGAAGAAATTAGGCCTAATACTGTGACAGTGGCTAGTATTCTGTCAGTTTGTGCTCAGTTGTCAATCATGATGTTGTGTAAGGAGGTTCATGGTTACCTATTACGGCAAGAATTTGAGTCCAATGTTCTTGTGAGCAATTCCCTGATGACTACCTATGCAAAATGTGGAGACATAAAGAGCTCAAGGAGTGTGTTTGTGAAAATGCCTGAAAGAAATTTAGTTTCATGGAACTCAATTCTTCTGGGGTTAGGAATGCATGGCCATGCAGATGAAACATTTGCTTTATTTGAAAAAATGGAGGCGGCAGGATTGGAGCCTGATCATGCAACTTTTACAGCTCTTCTTTCTGCTTGCAGCCATGCAGGGAGGATAGAAGAGGGGCTGAAGTATTTTCAGCGTATGGTAGAAGTTTACAAAATTGAGCCTCAACTTGAACAGTACACCTGCATGGTTGATCTTTTAGGCCGAGCTGGCCATCTAAAGCAAGCATATGATATTATTTCAACAATGCCTTGTGCCTCCGATGATCGTATATGGGGATCATTGCTTGGATCATGCAAAATTCATGGTGATGAAAGACTGGCAGAAGTTGTGGCTGATCATATCTTTAAACTGGATCCTACTAATATTGGCTACCGTACACTTCTTGCAAATTTGTACGAAGATTATGGGAAATGGAATGAAGTTACCAGGGTTAGATCAGACATCAAAGGTGTGGGACTCAAGAAGATGCCTGGGTGTAGTTGGATTGAAGTTGATAACAATCTCCATACCTTTACTGCAGGTGATCATTCTCATAACGAGTCAGAGGAGATATATTTTACAATAGAAAATCTGACAACTGAAATTAGGAAGGCAGGATATATCCCTCAATTACTGACTCTGGGTGAGGGGCTTGATGAGGTTCTTGATGAAGATCTTACGTCATCTAGAGACAAGGGTTTGTTGTTTCTGGTTGCAAGTACAAACAATCACCAACAAGTTTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

844

Amino Acids

94.05

Weight (kDa)

5.78

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 178 - 206 9e-07 PPR repeat
PPR_2 PF13041 283 - 329 6e-08 PPR repeat family
PPR PF01535 285 - 314 2.8e-07 PPR repeat
PPR_2 PF13041 485 - 532 1.2e-09 PPR repeat family
PPR PF01535 487 - 514 4.2e-06 PPR repeat
PPR_2 PF13041 586 - 633 1.3e-10 PPR repeat family
PPR_long PF17177 602 - 684 5.2e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 703 - 765 2.1e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014735)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05260
malus_domestica MD06G1174400.v1.1 MD06G1174600.v1.1
prunus_persica Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1
pyrus_communis pycom06g15570
rosa_chinensis RchiOBHm_Chr7g0193611
rosa_laevigata RLG00000004256
rosa_multiflora Rmu_sc0042944.1_g000002
rosa_roxburghii Rroxscaffold_3G00261960
rosa_rugosa Rorug07G0011000 Rorug07G0011100
rosa_samantha Rh7AG137600 Rh7BG137800 Rh7CG141400
rosa_wichuraiana Rw7G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1994, 2315
Acc36I ACCTGC 2 cut(s) 1994, 2315
AccB1I GGYRCC 1 cut(s) 1166
AccIII TCCGGA 1 cut(s) 1481
AciI CCGC 6 cut(s) 284, 800, 908, 1170, 1282, 1844
AclWI GGATC 6 cut(s) 624, 1015, 2089, 2101, 2153, 2166
AcoI YGGCCR 4 cut(s) 228, 969, 1801, 2014
AcuI CTGAAG 2 cut(s) 1114, 1946
AcyI GRCGYC 2 cut(s) 95, 1167
AdeI CACNNNGTG 1 cut(s) 666
AfaI GTAC 8 cut(s) 251, 773, 1062, 1111, 1982, 2182, 2201, 2506
AfiI CCNNNNNNNGG 3 cut(s) 189, 366, 571
AflIII ACRYGT 1 cut(s) 1086
AhlI ACTAGT 1 cut(s) 1336
AjnI CCWGG 4 cut(s) 153, 1296, 2231, 2275
AleI CACNNNNGTG 1 cut(s) 965
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 9 cut(s) 238, 323, 585, 1076, 1448, 1514, 1722, 1880, 2012
AluI AGCT 9 cut(s) 238, 323, 585, 1076, 1448, 1514, 1722, 1880, 2012
Alw21I GWGCWC 6 cut(s) 587, 671, 989, 1043, 1597, 1724
Alw26I GTCTC 4 cut(s) 13, 889, 1704, 2469
AlwI GGATC 6 cut(s) 624, 1015, 2089, 2101, 2153, 2166
AlwNI CAGNNNCTG 1 cut(s) 154
Aor13HI TCCGGA 1 cut(s) 1481
AoxI GGCC 8 cut(s) 228, 357, 899, 969, 1553, 1801, 2005, 2014
ApeKI GCWGC 2 cut(s) 5, 1896
AspLEI GCGC 1 cut(s) 1169
AspS9I GGNCC 1 cut(s) 1137
AsuC2I CCSGG 1 cut(s) 211
AsuHPI GGTGA 9 cut(s) 245, 749, 839, 983, 1115, 2123, 2339, 2441, 2507
AvaII GGWCC 1 cut(s) 1137
AxyI CCTNAGG 1 cut(s) 903
BalI TGGCCA 3 cut(s) 971, 1803, 2016
BamHI GGATCC 1 cut(s) 2158
BanI GGYRCC 1 cut(s) 1166
BanII GRGCYC 2 cut(s) 587, 1724
BauI CACGAG 2 cut(s) 31, 1203
Bbv12I GWGCWC 6 cut(s) 587, 671, 989, 1043, 1597, 1724
BbvCI CCTCAGC 1 cut(s) 324
BbvI GCAGC 1 cut(s) 1908
BccI CCATC 7 cut(s) 55, 172, 601, 728, 1181, 1312, 2025
BceAI ACGGC 3 cut(s) 479, 701, 1661
BciT130I CCWGG 4 cut(s) 155, 1298, 2233, 2277
BclI TGATCA 5 cut(s) 240, 739, 1861, 2140, 2329
BcnI CCSGG 1 cut(s) 211
BcoDI GTCTC 4 cut(s) 13, 889, 1704, 2469
BcuI ACTAGT 1 cut(s) 1336
BfaI CTAG 7 cut(s) 390, 923, 1337, 1449, 1505, 1575, 2472
BfmI CTRYAG 3 cut(s) 318, 1311, 2322
BfoI RGCGCY 1 cut(s) 1170
BfuAI ACCTGC 2 cut(s) 1994, 2315
BglI GCCNNNNNGGC 1 cut(s) 2013
BglII AGATCT 1 cut(s) 2458
BisI GCNGC 4 cut(s) 6, 1170, 1845, 1897
BlsI GCNGC 4 cut(s) 7, 1171, 1846, 1898
Bme1390I CCNGG 5 cut(s) 155, 211, 1298, 2233, 2277
Bme18I GGWCC 1 cut(s) 1137
BmgT120I GGNCC 1 cut(s) 1137
BmiI GGNNCC 5 cut(s) 54, 1139, 1168, 1857, 2160
BmrFI CCNGG 5 cut(s) 155, 211, 1298, 2233, 2277
BmsI GCATC 4 cut(s) 265, 433, 481, 2262
Bpu10I CCTNAGC 1 cut(s) 324
BpuEI CTTGAG 3 cut(s) 1173, 1708, 2249
BpuMI CCSGG 1 cut(s) 211
BsaBI GATNNNNATC 3 cut(s) 738, 2304, 2457
BsaHI GRCGYC 2 cut(s) 95, 1167
BsaI GGTCTC 1 cut(s) 13
BsaJI CCNNGG 4 cut(s) 154, 1296, 2232, 2276
BsaWI WCCGGW 1 cut(s) 1481
Bsc4I CCNNNNNNNGG 3 cut(s) 189, 366, 571
Bse118I RCCGGY 1 cut(s) 230
Bse1I ACTGG 4 cut(s) 1120, 1374, 2127, 2160
Bse21I CCTNAGG 1 cut(s) 903
Bse3DI GCAATG 1 cut(s) 2084
Bse8I GATNNNNATC 3 cut(s) 738, 2304, 2457
BseAI TCCGGA 1 cut(s) 1481
BseBI CCWGG 4 cut(s) 155, 1298, 2233, 2277
BseDI CCNNGG 4 cut(s) 154, 1296, 2232, 2276
BseGI GGATG 9 cut(s) 280, 496, 600, 612, 739, 793, 1198, 1292, 1323
BseJI GATNNNNATC 3 cut(s) 738, 2304, 2457
BseLI CCNNNNNNNGG 3 cut(s) 189, 366, 571
BseMI GCAATG 1 cut(s) 2084
BseMII CTCAG 6 cut(s) 117, 315, 510, 917, 1505, 1610
BseNI ACTGG 4 cut(s) 1120, 1374, 2127, 2160
BseRI GAGGAG 1 cut(s) 2366
BseX3I CGGCCG 1 cut(s) 228
BseXI GCAGC 1 cut(s) 1908
Bsh1285I CGRYCG 1 cut(s) 231
BshFI GGCC 8 cut(s) 230, 359, 901, 971, 1555, 1803, 2007, 2016
BshNI GGYRCC 1 cut(s) 1166
BsiEI CGRYCG 1 cut(s) 231
BsiHKAI GWGCWC 6 cut(s) 587, 671, 989, 1043, 1597, 1724
BsiSI CCGG 3 cut(s) 211, 231, 1482
BslFI GGGAC 2 cut(s) 1123, 2274
BslI CCNNNNNNNGG 3 cut(s) 189, 366, 571
BsmAI GTCTC 4 cut(s) 13, 889, 1704, 2469
BsmFI GGGAC 2 cut(s) 1123, 2274
BsmI GAATGC 5 cut(s) 802, 1007, 1474, 1523, 1800
BsnI GGCC 8 cut(s) 230, 359, 901, 971, 1555, 1803, 2007, 2016
Bso31I GGTCTC 1 cut(s) 13
Bsp1286I GDGCHC 6 cut(s) 587, 671, 989, 1043, 1597, 1724
Bsp13I TCCGGA 1 cut(s) 1481
Bsp1407I TGTACA 1 cut(s) 771
BspACI CCGC 6 cut(s) 284, 800, 908, 1170, 1282, 1844
BspANI GGCC 8 cut(s) 230, 359, 901, 971, 1555, 1803, 2007, 2016
BspCNI CTCAG 6 cut(s) 116, 316, 511, 916, 1504, 1609
BspEI TCCGGA 1 cut(s) 1481
BspHI TCATGA 2 cut(s) 1225, 1608
BspLI GGNNCC 5 cut(s) 54, 1139, 1168, 1857, 2160
BspMAI CTGCAG 1 cut(s) 2326
BspMI ACCTGC 2 cut(s) 1994, 2315
BspPI GGATC 6 cut(s) 624, 1015, 2089, 2101, 2153, 2166
BspQI GCTCTTC 2 cut(s) 676, 1887
BspT107I GGYRCC 1 cut(s) 1166
BspTNI GGTCTC 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 2084
BsrFI RCCGGY 1 cut(s) 230
BsrGI TGTACA 1 cut(s) 771
BsrI ACTGG 4 cut(s) 1120, 1374, 2127, 2160
BssAI RCCGGY 1 cut(s) 230
BssECI CCNNGG 4 cut(s) 154, 1296, 2232, 2276
BssNI GRCGYC 2 cut(s) 95, 1167
BssSI CACGAG 2 cut(s) 31, 1203
Bst2BI CACGAG 2 cut(s) 31, 1203
Bst2UI CCWGG 4 cut(s) 155, 1298, 2233, 2277
Bst4CI ACNGT 6 cut(s) 150, 853, 1564, 1570, 1980, 2180
Bst6I CTCTTC 3 cut(s) 676, 1887, 1911
BstACI GRCGYC 2 cut(s) 95, 1167
BstAUI TGTACA 1 cut(s) 771
BstC8I GCNNGC 5 cut(s) 646, 1284, 1894, 2014, 2028
BstDEI CTNAG 7 cut(s) 41, 103, 324, 519, 903, 1491, 1596
BstEII GGTNACC 1 cut(s) 1634
BstF5I GGATG 9 cut(s) 280, 496, 600, 612, 739, 793, 1198, 1292, 1323
BstH2I RGCGCY 1 cut(s) 1170
BstHHI GCGC 1 cut(s) 1169
BstMAI GTCTC 4 cut(s) 13, 889, 1704, 2469
BstMCI CGRYCG 1 cut(s) 231
BstMWI GCNNNNNNNGC 2 cut(s) 1520, 2013
BstNI CCWGG 4 cut(s) 155, 1298, 2233, 2277
BstNSI RCATGY 3 cut(s) 410, 1090, 1537
BstPI GGTNACC 1 cut(s) 1634
BstSCI CCNGG 5 cut(s) 153, 209, 1296, 2231, 2275
BstSFI CTRYAG 3 cut(s) 318, 1311, 2322
BstV1I GCAGC 1 cut(s) 1908
BstX2I RGATCY 3 cut(s) 1020, 2158, 2458
BstXI CCANNNNNNTGG 1 cut(s) 967
BstYI RGATCY 3 cut(s) 1020, 2158, 2458
BstZI CGGCCG 1 cut(s) 228
Bsu36I CCTNAGG 1 cut(s) 903
BsuRI GGCC 8 cut(s) 230, 359, 901, 971, 1555, 1803, 2007, 2016
BtgZI GCGATG 1 cut(s) 447
BtsCI GGATG 9 cut(s) 280, 496, 600, 612, 739, 793, 1198, 1292, 1323
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 5 cut(s) 61, 858, 1053, 1381, 1575
BveI ACCTGC 2 cut(s) 1994, 2315
Cac8I GCNNGC 5 cut(s) 646, 1284, 1894, 2014, 2028
CaiI CAGNNNCTG 1 cut(s) 154
CciI TCATGA 2 cut(s) 1225, 1608
CfoI GCGC 1 cut(s) 1169
Cfr10I RCCGGY 1 cut(s) 230
Cfr13I GGNCC 1 cut(s) 1137
CseI GACGC 1 cut(s) 103
Csp6I GTAC 8 cut(s) 250, 772, 1061, 1110, 1981, 2181, 2200, 2505
CviQI GTAC 8 cut(s) 250, 772, 1061, 1110, 1981, 2181, 2200, 2505
DdeI CTNAG 7 cut(s) 41, 103, 324, 519, 903, 1491, 1596
DinI GGCGCC 1 cut(s) 1168
DraI TTTAAA 2 cut(s) 1518, 2152
DraIII CACNNNGTG 1 cut(s) 666
EaeI YGGCCR 4 cut(s) 228, 969, 1801, 2014
EagI CGGCCG 1 cut(s) 228
Eam1104I CTCTTC 3 cut(s) 676, 1887, 1911
EarI CTCTTC 3 cut(s) 676, 1887, 1911
Ecl136II GAGCTC 2 cut(s) 585, 1722
EclXI CGGCCG 1 cut(s) 228
Eco147I AGGCCT 2 cut(s) 359, 1555
Eco24I GRGCYC 2 cut(s) 587, 1724
Eco31I GGTCTC 1 cut(s) 13
Eco32I GATATC 2 cut(s) 552, 1479
Eco47I GGWCC 1 cut(s) 1137
Eco52I CGGCCG 1 cut(s) 228
Eco53kI GAGCTC 2 cut(s) 585, 1722
Eco57I CTGAAG 2 cut(s) 1114, 1946
Eco81I CCTNAGG 1 cut(s) 903
Eco91I GGTNACC 1 cut(s) 1634
EcoICRI GAGCTC 2 cut(s) 585, 1722
EcoO109I RGGNCCY 1 cut(s) 1137
EcoO65I GGTNACC 1 cut(s) 1634
EcoRI GAATTC 1 cut(s) 178
EcoRII CCWGG 4 cut(s) 153, 1296, 2231, 2275
EcoRV GATATC 2 cut(s) 552, 1479
EcoT22I ATGCAT 1 cut(s) 1800
EcoT38I GRGCYC 2 cut(s) 587, 1724
EgeI GGCGCC 1 cut(s) 1168
EheI GGCGCC 1 cut(s) 1168
FaqI GGGAC 2 cut(s) 1123, 2274
FauI CCCGC 1 cut(s) 1275
FauNDI CATATG 1 cut(s) 2032
FbaI TGATCA 5 cut(s) 240, 739, 1861, 2140, 2329
Fnu4HI GCNGC 4 cut(s) 6, 1170, 1845, 1897
FokI GGATG 9 cut(s) 287, 503, 607, 619, 746, 800, 1185, 1279, 1330
FriOI GRGCYC 2 cut(s) 587, 1724
Fsp4HI GCNGC 4 cut(s) 6, 1170, 1845, 1897
FspBI CTAG 7 cut(s) 390, 923, 1337, 1449, 1505, 1575, 2472
GlaI GCGC 1 cut(s) 1168
GluI GCNGC 4 cut(s) 6, 1170, 1845, 1897
HaeII RGCGCY 1 cut(s) 1170
HaeIII GGCC 8 cut(s) 230, 359, 901, 971, 1555, 1803, 2007, 2016
HapII CCGG 3 cut(s) 211, 231, 1482
HgaI GACGC 1 cut(s) 103
HhaI GCGC 1 cut(s) 1169
Hin1I GRCGYC 2 cut(s) 95, 1167
Hin6I GCGC 1 cut(s) 1167
HinP1I GCGC 1 cut(s) 1167
HindIII AAGCTT 1 cut(s) 1512
HpaII CCGG 3 cut(s) 211, 231, 1482
HphI GGTGA 9 cut(s) 245, 749, 839, 983, 1115, 2123, 2339, 2441, 2507
Hpy166II GTNNAC 4 cut(s) 439, 1954, 1983, 2183
Hpy8I GTNNAC 4 cut(s) 439, 1954, 1983, 2183
Hpy99I CGWCG 1 cut(s) 97
HpyAV CCTTC 2 cut(s) 1128, 2389
HpyCH4III ACNGT 6 cut(s) 150, 853, 1564, 1570, 1980, 2180
HpyCH4IV ACGT 2 cut(s) 252, 2465
HpyF10VI GCNNNNNNNGC 2 cut(s) 1520, 2013
HpyF3I CTNAG 7 cut(s) 41, 103, 324, 519, 903, 1491, 1596
HpySE526I ACGT 2 cut(s) 252, 2465
Hsp92I GRCGYC 2 cut(s) 95, 1167
HspAI GCGC 1 cut(s) 1167
KasI GGCGCC 1 cut(s) 1166
Kpn2I TCCGGA 1 cut(s) 1481
Ksp22I TGATCA 5 cut(s) 240, 739, 1861, 2140, 2329
LguI GCTCTTC 2 cut(s) 676, 1887
LmnI GCTCC 3 cut(s) 52, 816, 1855
Lsp1109I GCAGC 1 cut(s) 1908
LweI GCATC 4 cut(s) 265, 433, 481, 2262
MaeI CTAG 7 cut(s) 390, 923, 1337, 1449, 1505, 1575, 2472
MaeII ACGT 2 cut(s) 252, 2465
MaeIII GTNAC 5 cut(s) 945, 1148, 1564, 1634, 2227
MfeI CAATTG 2 cut(s) 936, 1046
MflI RGATCY 3 cut(s) 1020, 2158, 2458
MhlI GDGCHC 6 cut(s) 587, 671, 989, 1043, 1597, 1724
MlsI TGGCCA 3 cut(s) 971, 1803, 2016
MluNI TGGCCA 3 cut(s) 971, 1803, 2016
Mly113I GGCGCC 1 cut(s) 1167
MlyI GAGTC 5 cut(s) 1424, 1667, 2256, 2354, 2416
MmeI TCCRAC 2 cut(s) 584, 2267
Mox20I TGGCCA 3 cut(s) 971, 1803, 2016
Mph1103I ATGCAT 1 cut(s) 1800
MroI TCCGGA 1 cut(s) 1481
MscI TGGCCA 3 cut(s) 971, 1803, 2016
MseI TTAA 5 cut(s) 294, 354, 1305, 1517, 2151
MslI CAYNNNNRTG 8 cut(s) 405, 965, 1157, 1203, 1803, 1809, 1988, 2112
Msp20I TGGCCA 3 cut(s) 971, 1803, 2016
MspI CCGG 3 cut(s) 211, 231, 1482
MspR9I CCNGG 5 cut(s) 155, 211, 1298, 2233, 2277
MunI CAATTG 2 cut(s) 936, 1046
Mva1269I GAATGC 5 cut(s) 802, 1007, 1474, 1523, 1800
MvaI CCWGG 4 cut(s) 155, 1298, 2233, 2277
MwoI GCNNNNNNNGC 2 cut(s) 1520, 2013
NarI GGCGCC 1 cut(s) 1167
NciI CCSGG 1 cut(s) 211
NdeI CATATG 1 cut(s) 2032
NlaIV GGNNCC 5 cut(s) 54, 1139, 1168, 1857, 2160
NmeAIII GCCGAG 1 cut(s) 2033
NmuCI GTSAC 1 cut(s) 1564
NsiI ATGCAT 1 cut(s) 1800
NspI RCATGY 3 cut(s) 410, 1090, 1537
OliI CACNNNNGTG 1 cut(s) 965
PagI TCATGA 2 cut(s) 1225, 1608
PaqCI CACCTGC 2 cut(s) 1994, 2315
PceI AGGCCT 2 cut(s) 359, 1555
PciI ACATGT 1 cut(s) 1086
PciSI GCTCTTC 2 cut(s) 676, 1887
PctI GAATGC 5 cut(s) 802, 1007, 1474, 1523, 1800
PfeI GAWTC 6 cut(s) 397, 703, 1144, 1216, 1256, 1355
PfoI TCCNGGA 1 cut(s) 209
PkrI GCNGC 4 cut(s) 7, 1171, 1846, 1898
PleI GAGTC 5 cut(s) 1423, 1666, 2256, 2353, 2416
PluTI GGCGCC 1 cut(s) 1170
PpsI GAGTC 5 cut(s) 1423, 1666, 2256, 2353, 2416
PpuMI RGGWCCY 1 cut(s) 1137
PscI ACATGT 1 cut(s) 1086
Psp124BI GAGCTC 2 cut(s) 587, 1724
Psp5II RGGWCCY 1 cut(s) 1137
Psp6I CCWGG 4 cut(s) 153, 1296, 2231, 2275
PspEI GGTNACC 1 cut(s) 1634
PspGI CCWGG 4 cut(s) 153, 1296, 2231, 2275
PspN4I GGNNCC 5 cut(s) 54, 1139, 1168, 1857, 2160
PspPI GGNCC 1 cut(s) 1137
PspPPI RGGWCCY 1 cut(s) 1137
PstI CTGCAG 1 cut(s) 2326
PstNI CAGNNNCTG 1 cut(s) 154
PsuI RGATCY 3 cut(s) 1020, 2158, 2458
RsaI GTAC 8 cut(s) 251, 773, 1062, 1111, 1982, 2182, 2201, 2506
RsaNI GTAC 8 cut(s) 250, 772, 1061, 1110, 1981, 2181, 2200, 2505
RseI CAYNNNNRTG 8 cut(s) 405, 965, 1157, 1203, 1803, 1809, 1988, 2112
SacI GAGCTC 2 cut(s) 587, 1724
SapI GCTCTTC 2 cut(s) 676, 1887
SaqAI TTAA 5 cut(s) 294, 354, 1305, 1517, 2151
SatI GCNGC 4 cut(s) 6, 1170, 1845, 1897
Sau96I GGNCC 1 cut(s) 1137
SchI GAGTC 5 cut(s) 1424, 1667, 2256, 2354, 2416
ScrFI CCNGG 5 cut(s) 155, 211, 1298, 2233, 2277
SduI GDGCHC 6 cut(s) 587, 671, 989, 1043, 1597, 1724
SfaNI GCATC 4 cut(s) 265, 433, 481, 2262
SfcI CTRYAG 3 cut(s) 318, 1311, 2322
SfiI GGCCNNNNNGGCC 1 cut(s) 2013
SfoI GGCGCC 1 cut(s) 1168
SinI GGWCC 1 cut(s) 1137
SmiMI CAYNNNNRTG 8 cut(s) 405, 965, 1157, 1203, 1803, 1809, 1988, 2112
SmlI CTYRAG 3 cut(s) 1188, 1723, 2264
SmoI CTYRAG 3 cut(s) 1188, 1723, 2264
SpeI ACTAGT 1 cut(s) 1336
SseBI AGGCCT 2 cut(s) 359, 1555
SsiI CCGC 6 cut(s) 284, 800, 908, 1170, 1282, 1844
SspDI GGCGCC 1 cut(s) 1166
SspI AATATT 1 cut(s) 2170
SspMI CTAG 7 cut(s) 390, 923, 1337, 1449, 1505, 1575, 2472
SstI GAGCTC 2 cut(s) 587, 1724
StuI AGGCCT 2 cut(s) 359, 1555
StyD4I CCNGG 5 cut(s) 153, 209, 1296, 2231, 2275
TaaI ACNGT 6 cut(s) 150, 853, 1564, 1570, 1980, 2180
TaiI ACGT 2 cut(s) 255, 2468
TaqI TCGA 3 cut(s) 176, 197, 452
TatI WGTACW 4 cut(s) 771, 1109, 1980, 2504
TauI GCSGC 2 cut(s) 1172, 1847
TfiI GAWTC 6 cut(s) 397, 703, 1144, 1216, 1256, 1355
Tru1I TTAA 5 cut(s) 294, 354, 1305, 1517, 2151
Tru9I TTAA 5 cut(s) 294, 354, 1305, 1517, 2151
TscAI CASTG 5 cut(s) 68, 858, 1060, 1381, 1575
TseFI GTSAC 1 cut(s) 1564
TseI GCWGC 2 cut(s) 5, 1896
Tsp45I GTSAC 1 cut(s) 1564
TspRI CASTG 5 cut(s) 68, 858, 1060, 1381, 1575
VpaK11BI GGWCC 1 cut(s) 1137
XbaI TCTAGA 3 cut(s) 922, 1504, 2471
XceI RCATGY 3 cut(s) 410, 1090, 1537
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XspI CTAG 7 cut(s) 390, 923, 1337, 1449, 1505, 1575, 2472
Zsp2I ATGCAT 1 cut(s) 1800
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.