Rw7G011720

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014735
Physical Location & Seq
Forward (+)
11016036 .. 11020296
4261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G011720.1

Sequence Viewer

Length: 2502 bp
ATGGCTGGTGTGAGAGTGAGACCACAAGGCTCGTGTTCGCTGAGACCATCGGAGCCATTACACTGCTACAAGTTGAGAGGTGGTGCTACAACAGTTTCTTTTGCTTCAAAGTTTCAATCTTTATGTTGCAAATCACAGTTCCTGGGAAACCCATCTCGGAAATCGAGGAATTGTTCCCGGATTGATGTTGTTTCGGCCGGTGAGCTGATCAAGAGGTACGTTGAGAATGGGAATTGCGAGGATGCGATAGTGGTTTATGTTAAAATGCTTGAACTTGGTTTGCCTGTAGCTGAGGAATTTCAGTTCTTTCCGATTTTGATTAAGGCCTTTGGGGGGCTTTGTGATGTGGGCAAGGCTAGGGAGATTCATGGGCATGTGTTGAAATTGGGGGTTTTGGGCGATGTTTACGATGCCAATTCGATTTTGGGGGTTTATTGGAAATGTGGGGAGGTTGAGGATGCGGTTCAGATGTTTGAGAAAATGCCTGAGAGGGATTTGGTGTCTTGGAATACGATGATATCTGGGTTTTGCCGTTCAGGGGATTATGTGAGCTCGTTGGGGATGTTTAGTCGGATGGTTCGTGATCATTGGGTGTTGCCTAACCGGGTGGCTTGCCTTTCGGCTCTCACTTCGTGCTCTTCGGTTCACTCTTTGGTTCATGGCAGAGAGATTCATGGCTTTGCCCTGAAGCGTTGGCTGGATGGTGATCAGTTTATGGTTAGTGGGTTGATTGATATGTACATGAAATGTGGGGATGTGAAGAATGCGGAATGTGTTTATAGGAGCGTTATTGGTGAAGCGTGTATTAGAGAAAATACAGTGATATGGAATGTGATGATTTCGGGCTATGTCTCTAATGGGTGTTGGCCGCAGGCGGTGGACTTGTTTCTAGAGATGTTGGCGATTGGGTTCTTACCCGATACTTCCACAATGGTGGCCATTACAGGTTTGTGCTCTCACCTAATGGATTTAGCATTTGGAAAGCAGATCCATAAATTCTGTTTGAGCACTCAATTGAACACTGATGTACGAGTTGAAACAGCTCTTATGGACATGTATTTCAAATGTGGTGATAGTACAACTGGTCTTGAAATCTTCAGAAGGTCCCTGAATCGTAACATGGTCATGTGGGGCGCTGCCATCTCAAATTTTGCTCAAAGTAGTCATCCTCGTGAGGCATTGAATCTGTTTCATGACTATATTTTAGAATATGGCTTTGTGGATTCTGTCATAATCTTGGCTGTTCTGCGGGCTTGTTCATCCCTGGCTGTTAAGTCTATAGGGATGGAAATTCATGGGCTACTAGTGAAGTTGGGGTTTGATTCAGACCATTTTGTTGCCAGTGCATTAGTTGATTTTTATTCAAAAACCAGAGATATTGAGTCTGCTCAAAAAGTATTTCACAGACTACCGGCTAGAGATTTGGTGTCATGGAATGCCTTGATATCCGGATATACTCAGAATGAGTATCTAGATGAAGCTTTAAAGGCATTCCTTGACATGCAACTTGAAGAAATTAGACCTAATACTGTGACAGTGGCTAGTATTCTGTCAGTTTGTGCTCAGTTGTCAATCATGATGTTGTGTAAGGAGGTTCATGGTTACCTATTACGGCAAGAATTTGAGTCCAATGTTCTTGTGAGCAATTCCCTGATGACTACGTATGCAAAATGTGGAGACATAAAGAGCTCAAGGAGTGTGTTTGTGAAAATGCCTGAAAGAAATTTAGTTTCATGGAACTCAATTCTTCTGGGGTTAGGAGTGCATGGCCATGCAGATGAAACATTTGCTTTATTTGAAAAAATGGAAGCGGCAGGATTGGAGCCTGATCATGCAACTTTTACAGCTCTTCTTTCTGCTTGCAGCCATGCAGGGAGGATAGAAGAGGGGCTGAAGTATTTTCAGCGTATGGTAGAAGTTTACAAGATTGAGCCTCAACTTGAACAGTACACCTGCATGGTTGATCTTTTAGGCCGAGCTGGCCATCTAAAGCAAGCATATGATATTATTTCAACAATGCCTTGTGCCTCCGATGATCGTATATGGGGATCATTGCTTGGATCATGCAAAATTCATGGTGATGAAAGACTGGCAGAAGTTGTGGCTGATCATATCTTTAAACTGGATCCTACTAATATTGGCTACCGTACACTTCTTGCAAATTTGTACGAAGATTATGGGAAATGGAATGAAGTTACCAGGGTTAGATCAGACATCAAAGGTGTGGGACTCAAGAAGATGCCTGGGTGTAGTTGGATTGAAGTTGATAACAATCTCCATACCTTTACTGCAGGTGATCATTCTCATAACGAGTCAGAGGAGATATATTTTACAATAGAAAATCTGACAACTGAAATTAGGAAGGCAGGATATATCCCTCAATTACTGACTCTGGGTGAGGGGCTTGATGAGGTTCTTGATGAAGATCTTACGTCATCTAGAGACAAGGGTTTGTTGTTTCTGGTTGCAAGTACAAACAATCACCAACAAGTTTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

833

Amino Acids

92.98

Weight (kDa)

5.74

Isoelectric Point (pI)

40.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 167 - 195 2.7e-07 PPR repeat
PPR_2 PF13041 272 - 318 5.5e-08 PPR repeat family
PPR PF01535 274 - 304 3.8e-07 PPR repeat
PPR_2 PF13041 474 - 521 1.2e-09 PPR repeat family
PPR PF01535 476 - 503 4.2e-06 PPR repeat
PPR_2 PF13041 575 - 622 2.7e-10 PPR repeat family
PPR_long PF17177 592 - 673 6.6e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 692 - 754 2.1e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014735)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05260
malus_domestica MD06G1174400.v1.1 MD06G1174600.v1.1
prunus_persica Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1
pyrus_communis pycom06g15570
rosa_chinensis RchiOBHm_Chr7g0193611
rosa_laevigata RLG00000004256
rosa_multiflora Rmu_sc0042944.1_g000002
rosa_roxburghii Rroxscaffold_3G00261960
rosa_rugosa Rorug07G0011000 Rorug07G0011100
rosa_samantha Rh7AG137600 Rh7BG137800 Rh7CG141400
rosa_wichuraiana Rw7G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1961, 2282
Acc36I ACCTGC 2 cut(s) 1961, 2282
AccIII TCCGGA 1 cut(s) 1448
AciI CCGC 6 cut(s) 461, 767, 869, 875, 1249, 1811
AclWI GGATC 5 cut(s) 982, 2056, 2068, 2120, 2133
AcoI YGGCCR 5 cut(s) 195, 866, 936, 1768, 1981
AcsI RAATTY 8 cut(s) 296, 995, 1147, 1290, 1619, 1723, 2070, 2161
AcuI CTGAAG 3 cut(s) 707, 1081, 1913
AdeI CACNNNGTG 1 cut(s) 633
AfaI GTAC 8 cut(s) 218, 740, 1029, 1078, 1949, 2149, 2168, 2473
AfiI CCNNNNNNNGG 2 cut(s) 333, 538
AflIII ACRYGT 1 cut(s) 1053
AhlI ACTAGT 1 cut(s) 1303
AjnI CCWGG 4 cut(s) 141, 1263, 2198, 2242
AleI CACNNNNGTG 1 cut(s) 932
AloI GAACNNNNNNTCC 2 cut(s) 287, 319
AluBI AGCT 8 cut(s) 205, 290, 552, 1043, 1481, 1689, 1847, 1979
AluI AGCT 8 cut(s) 205, 290, 552, 1043, 1481, 1689, 1847, 1979
Alw21I GWGCWC 6 cut(s) 554, 638, 956, 1010, 1564, 1691
Alw26I GTCTC 5 cut(s) 13, 37, 856, 1671, 2436
AlwI GGATC 5 cut(s) 982, 2056, 2068, 2120, 2133
AlwNI CAGNNNCTG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 1448
AoxI GGCC 7 cut(s) 195, 324, 866, 936, 1768, 1972, 1981
ApeKI GCWGC 2 cut(s) 1136, 1863
ApoI RAATTY 8 cut(s) 296, 995, 1147, 1290, 1619, 1723, 2070, 2161
AspLEI GCGC 1 cut(s) 1136
AspS9I GGNCC 1 cut(s) 1104
AsuC2I CCSGG 2 cut(s) 178, 605
AsuHPI GGTGA 9 cut(s) 212, 716, 806, 950, 1082, 2090, 2306, 2408, 2474
AvaII GGWCC 1 cut(s) 1104
BalI TGGCCA 3 cut(s) 938, 1770, 1983
BamHI GGATCC 1 cut(s) 2125
BanII GRGCYC 2 cut(s) 554, 1691
BauI CACGAG 2 cut(s) 31, 1170
Bbv12I GWGCWC 6 cut(s) 554, 638, 956, 1010, 1564, 1691
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 2 cut(s) 1123, 1875
BccI CCATC 7 cut(s) 55, 160, 568, 695, 1148, 1279, 1992
BceAI ACGGC 2 cut(s) 516, 1628
BciT130I CCWGG 4 cut(s) 143, 1265, 2200, 2244
BclI TGATCA 6 cut(s) 207, 583, 706, 1828, 2107, 2296
BcnI CCSGG 2 cut(s) 178, 605
BcoDI GTCTC 5 cut(s) 13, 37, 856, 1671, 2436
BcuI ACTAGT 1 cut(s) 1303
BfaI CTAG 7 cut(s) 357, 890, 1304, 1416, 1472, 1542, 2439
BfmI CTRYAG 3 cut(s) 285, 1278, 2289
BfoI RGCGCY 1 cut(s) 1137
BfuAI ACCTGC 2 cut(s) 1961, 2282
BglI GCCNNNNNGGC 1 cut(s) 1980
BglII AGATCT 1 cut(s) 2425
BisI GCNGC 4 cut(s) 869, 1137, 1812, 1864
BlsI GCNGC 4 cut(s) 870, 1138, 1813, 1865
Bme1390I CCNGG 6 cut(s) 143, 178, 605, 1265, 2200, 2244
Bme18I GGWCC 1 cut(s) 1104
BmgT120I GGNCC 1 cut(s) 1104
BmiI GGNNCC 4 cut(s) 54, 1106, 1824, 2127
BmrFI CCNGG 6 cut(s) 143, 178, 605, 1265, 2200, 2244
BmsI GCATC 4 cut(s) 232, 400, 448, 2229
Bpu10I CCTNAGC 1 cut(s) 291
BpuEI CTTGAG 2 cut(s) 1675, 2216
BpuMI CCSGG 2 cut(s) 178, 605
BsaAI YACGTR 1 cut(s) 1662
BsaBI GATNNNNATC 3 cut(s) 705, 2271, 2424
BsaI GGTCTC 2 cut(s) 13, 37
BsaJI CCNNGG 4 cut(s) 142, 1263, 2199, 2243
BsaWI WCCGGW 1 cut(s) 1448
Bsc4I CCNNNNNNNGG 2 cut(s) 333, 538
Bse118I RCCGGY 2 cut(s) 197, 1411
Bse1I ACTGG 4 cut(s) 1087, 1341, 2094, 2127
Bse3DI GCAATG 1 cut(s) 2051
Bse8I GATNNNNATC 3 cut(s) 705, 2271, 2424
BseAI TCCGGA 1 cut(s) 1448
BseBI CCWGG 4 cut(s) 143, 1265, 2200, 2244
BseDI CCNNGG 4 cut(s) 142, 1263, 2199, 2243
BseGI GGATG 9 cut(s) 247, 463, 567, 579, 706, 760, 1165, 1259, 1290
BseJI GATNNNNATC 3 cut(s) 705, 2271, 2424
BseLI CCNNNNNNNGG 2 cut(s) 333, 538
BseMI GCAATG 1 cut(s) 2051
BseMII CTCAG 5 cut(s) 32, 282, 477, 1472, 1577
BseNI ACTGG 4 cut(s) 1087, 1341, 2094, 2127
BseRI GAGGAG 1 cut(s) 2333
BseX3I CGGCCG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 1123, 1875
Bsh1285I CGRYCG 1 cut(s) 198
BshFI GGCC 7 cut(s) 197, 326, 868, 938, 1770, 1974, 1983
BsiEI CGRYCG 1 cut(s) 198
BsiHKAI GWGCWC 6 cut(s) 554, 638, 956, 1010, 1564, 1691
BsiSI CCGG 5 cut(s) 178, 198, 604, 1412, 1449
BslFI GGGAC 2 cut(s) 1090, 2241
BslI CCNNNNNNNGG 2 cut(s) 333, 538
BsmAI GTCTC 5 cut(s) 13, 37, 856, 1671, 2436
BsmFI GGGAC 2 cut(s) 1090, 2241
BsmI GAATGC 3 cut(s) 769, 1441, 1490
BsnI GGCC 7 cut(s) 197, 326, 868, 938, 1770, 1974, 1983
Bso31I GGTCTC 2 cut(s) 13, 37
Bsp1286I GDGCHC 6 cut(s) 554, 638, 956, 1010, 1564, 1691
Bsp13I TCCGGA 1 cut(s) 1448
Bsp1407I TGTACA 1 cut(s) 738
BspACI CCGC 6 cut(s) 461, 767, 869, 875, 1249, 1811
BspANI GGCC 7 cut(s) 197, 326, 868, 938, 1770, 1974, 1983
BspCNI CTCAG 5 cut(s) 33, 283, 478, 1471, 1576
BspEI TCCGGA 1 cut(s) 1448
BspHI TCATGA 2 cut(s) 1192, 1575
BspLI GGNNCC 4 cut(s) 54, 1106, 1824, 2127
BspMAI CTGCAG 1 cut(s) 2293
BspMI ACCTGC 2 cut(s) 1961, 2282
BspPI GGATC 5 cut(s) 982, 2056, 2068, 2120, 2133
BspQI GCTCTTC 2 cut(s) 643, 1854
BspTNI GGTCTC 2 cut(s) 13, 37
BsrDI GCAATG 1 cut(s) 2051
BsrFI RCCGGY 2 cut(s) 197, 1411
BsrGI TGTACA 1 cut(s) 738
BsrI ACTGG 4 cut(s) 1087, 1341, 2094, 2127
BssAI RCCGGY 2 cut(s) 197, 1411
BssECI CCNNGG 4 cut(s) 142, 1263, 2199, 2243
BssSI CACGAG 2 cut(s) 31, 1170
Bst2BI CACGAG 2 cut(s) 31, 1170
Bst2UI CCWGG 4 cut(s) 143, 1265, 2200, 2244
Bst4CI ACNGT 7 cut(s) 94, 138, 820, 1531, 1537, 1947, 2147
Bst6I CTCTTC 3 cut(s) 643, 1854, 1878
BstAUI TGTACA 1 cut(s) 738
BstBAI YACGTR 1 cut(s) 1662
BstC8I GCNNGC 6 cut(s) 613, 873, 1251, 1861, 1981, 1995
BstDEI CTNAG 5 cut(s) 41, 291, 486, 1458, 1563
BstEII GGTNACC 1 cut(s) 1601
BstF5I GGATG 9 cut(s) 247, 463, 567, 579, 706, 760, 1165, 1259, 1290
BstH2I RGCGCY 1 cut(s) 1137
BstHHI GCGC 1 cut(s) 1136
BstMAI GTCTC 5 cut(s) 13, 37, 856, 1671, 2436
BstMCI CGRYCG 1 cut(s) 198
BstMWI GCNNNNNNNGC 2 cut(s) 1487, 1980
BstNI CCWGG 4 cut(s) 143, 1265, 2200, 2244
BstNSI RCATGY 3 cut(s) 377, 1057, 1504
BstPI GGTNACC 1 cut(s) 1601
BstSCI CCNGG 6 cut(s) 141, 176, 603, 1263, 2198, 2242
BstSFI CTRYAG 3 cut(s) 285, 1278, 2289
BstSNI TACGTA 1 cut(s) 1662
BstV1I GCAGC 2 cut(s) 1123, 1875
BstX2I RGATCY 3 cut(s) 987, 2125, 2425
BstXI CCANNNNNNTGG 1 cut(s) 934
BstYI RGATCY 3 cut(s) 987, 2125, 2425
BstZI CGGCCG 1 cut(s) 195
BsuRI GGCC 7 cut(s) 197, 326, 868, 938, 1770, 1974, 1983
BtgZI GCGATG 1 cut(s) 414
BtsCI GGATG 9 cut(s) 247, 463, 567, 579, 706, 760, 1165, 1259, 1290
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 5 cut(s) 61, 825, 1020, 1348, 1542
BveI ACCTGC 2 cut(s) 1961, 2282
Cac8I GCNNGC 6 cut(s) 613, 873, 1251, 1861, 1981, 1995
CaiI CAGNNNCTG 1 cut(s) 142
CciI TCATGA 2 cut(s) 1192, 1575
CfoI GCGC 1 cut(s) 1136
Cfr10I RCCGGY 2 cut(s) 197, 1411
Cfr13I GGNCC 1 cut(s) 1104
Csp6I GTAC 8 cut(s) 217, 739, 1028, 1077, 1948, 2148, 2167, 2472
CviQI GTAC 8 cut(s) 217, 739, 1028, 1077, 1948, 2148, 2167, 2472
DdeI CTNAG 5 cut(s) 41, 291, 486, 1458, 1563
DraI TTTAAA 2 cut(s) 1485, 2119
DraIII CACNNNGTG 1 cut(s) 633
EaeI YGGCCR 5 cut(s) 195, 866, 936, 1768, 1981
EagI CGGCCG 1 cut(s) 195
Eam1104I CTCTTC 3 cut(s) 643, 1854, 1878
EarI CTCTTC 3 cut(s) 643, 1854, 1878
Ecl136II GAGCTC 2 cut(s) 552, 1689
EclXI CGGCCG 1 cut(s) 195
Eco105I TACGTA 1 cut(s) 1662
Eco147I AGGCCT 1 cut(s) 326
Eco24I GRGCYC 2 cut(s) 554, 1691
Eco31I GGTCTC 2 cut(s) 13, 37
Eco32I GATATC 2 cut(s) 519, 1446
Eco47I GGWCC 1 cut(s) 1104
Eco52I CGGCCG 1 cut(s) 195
Eco53kI GAGCTC 2 cut(s) 552, 1689
Eco57I CTGAAG 3 cut(s) 707, 1081, 1913
Eco91I GGTNACC 1 cut(s) 1601
EcoICRI GAGCTC 2 cut(s) 552, 1689
EcoO109I RGGNCCY 1 cut(s) 1104
EcoO65I GGTNACC 1 cut(s) 1601
EcoRII CCWGG 4 cut(s) 141, 1263, 2198, 2242
EcoRV GATATC 2 cut(s) 519, 1446
EcoT38I GRGCYC 2 cut(s) 554, 1691
FaqI GGGAC 2 cut(s) 1090, 2241
FauI CCCGC 1 cut(s) 1242
FauNDI CATATG 1 cut(s) 1999
FbaI TGATCA 6 cut(s) 207, 583, 706, 1828, 2107, 2296
Fnu4HI GCNGC 4 cut(s) 869, 1137, 1812, 1864
FokI GGATG 9 cut(s) 254, 470, 574, 586, 713, 767, 1152, 1246, 1297
FriOI GRGCYC 2 cut(s) 554, 1691
Fsp4HI GCNGC 4 cut(s) 869, 1137, 1812, 1864
FspBI CTAG 7 cut(s) 357, 890, 1304, 1416, 1472, 1542, 2439
GlaI GCGC 1 cut(s) 1135
GluI GCNGC 4 cut(s) 869, 1137, 1812, 1864
HaeII RGCGCY 1 cut(s) 1137
HaeIII GGCC 7 cut(s) 197, 326, 868, 938, 1770, 1974, 1983
HapII CCGG 5 cut(s) 178, 198, 604, 1412, 1449
HhaI GCGC 1 cut(s) 1136
Hin6I GCGC 1 cut(s) 1134
HinP1I GCGC 1 cut(s) 1134
HindIII AAGCTT 1 cut(s) 1479
HpaII CCGG 5 cut(s) 178, 198, 604, 1412, 1449
HphI GGTGA 9 cut(s) 212, 716, 806, 950, 1082, 2090, 2306, 2408, 2474
Hpy166II GTNNAC 6 cut(s) 406, 646, 880, 1921, 1950, 2150
Hpy8I GTNNAC 6 cut(s) 406, 646, 880, 1921, 1950, 2150
HpyAV CCTTC 2 cut(s) 1095, 2356
HpyCH4III ACNGT 7 cut(s) 94, 138, 820, 1531, 1537, 1947, 2147
HpyCH4IV ACGT 3 cut(s) 219, 1661, 2432
HpyF10VI GCNNNNNNNGC 2 cut(s) 1487, 1980
HpyF3I CTNAG 5 cut(s) 41, 291, 486, 1458, 1563
HpySE526I ACGT 3 cut(s) 219, 1661, 2432
HspAI GCGC 1 cut(s) 1134
Kpn2I TCCGGA 1 cut(s) 1448
Ksp22I TGATCA 6 cut(s) 207, 583, 706, 1828, 2107, 2296
LguI GCTCTTC 2 cut(s) 643, 1854
LmnI GCTCC 3 cut(s) 52, 783, 1822
Lsp1109I GCAGC 2 cut(s) 1123, 1875
LweI GCATC 4 cut(s) 232, 400, 448, 2229
MaeI CTAG 7 cut(s) 357, 890, 1304, 1416, 1472, 1542, 2439
MaeII ACGT 3 cut(s) 219, 1661, 2432
MaeIII GTNAC 4 cut(s) 1115, 1531, 1601, 2194
MfeI CAATTG 1 cut(s) 1013
MflI RGATCY 3 cut(s) 987, 2125, 2425
MhlI GDGCHC 6 cut(s) 554, 638, 956, 1010, 1564, 1691
MlsI TGGCCA 3 cut(s) 938, 1770, 1983
MluNI TGGCCA 3 cut(s) 938, 1770, 1983
MlyI GAGTC 5 cut(s) 1391, 1634, 2223, 2321, 2383
MmeI TCCRAC 2 cut(s) 551, 2234
Mox20I TGGCCA 3 cut(s) 938, 1770, 1983
MroI TCCGGA 1 cut(s) 1448
MscI TGGCCA 3 cut(s) 938, 1770, 1983
MseI TTAA 5 cut(s) 261, 321, 1272, 1484, 2118
MslI CAYNNNNRTG 8 cut(s) 372, 932, 1124, 1170, 1770, 1776, 1955, 2079
Msp20I TGGCCA 3 cut(s) 938, 1770, 1983
MspI CCGG 5 cut(s) 178, 198, 604, 1412, 1449
MspR9I CCNGG 6 cut(s) 143, 178, 605, 1265, 2200, 2244
MunI CAATTG 1 cut(s) 1013
Mva1269I GAATGC 3 cut(s) 769, 1441, 1490
MvaI CCWGG 4 cut(s) 143, 1265, 2200, 2244
MwoI GCNNNNNNNGC 2 cut(s) 1487, 1980
NciI CCSGG 2 cut(s) 178, 605
NdeI CATATG 1 cut(s) 1999
NlaIV GGNNCC 4 cut(s) 54, 1106, 1824, 2127
NmeAIII GCCGAG 1 cut(s) 2000
NmuCI GTSAC 1 cut(s) 1531
NspI RCATGY 3 cut(s) 377, 1057, 1504
OliI CACNNNNGTG 1 cut(s) 932
PagI TCATGA 2 cut(s) 1192, 1575
PaqCI CACCTGC 2 cut(s) 1961, 2282
PceI AGGCCT 1 cut(s) 326
PciI ACATGT 1 cut(s) 1053
PciSI GCTCTTC 2 cut(s) 643, 1854
PcsI WCGNNNNNNNCGW 1 cut(s) 577
PctI GAATGC 3 cut(s) 769, 1441, 1490
PfeI GAWTC 6 cut(s) 364, 670, 1111, 1183, 1223, 1322
PfoI TCCNGGA 1 cut(s) 176
PkrI GCNGC 4 cut(s) 870, 1138, 1813, 1865
PleI GAGTC 5 cut(s) 1390, 1633, 2223, 2320, 2383
PpsI GAGTC 5 cut(s) 1390, 1633, 2223, 2320, 2383
Ppu21I YACGTR 1 cut(s) 1662
PpuMI RGGWCCY 1 cut(s) 1104
PscI ACATGT 1 cut(s) 1053
Psp124BI GAGCTC 2 cut(s) 554, 1691
Psp5II RGGWCCY 1 cut(s) 1104
Psp6I CCWGG 4 cut(s) 141, 1263, 2198, 2242
PspEI GGTNACC 1 cut(s) 1601
PspGI CCWGG 4 cut(s) 141, 1263, 2198, 2242
PspN4I GGNNCC 4 cut(s) 54, 1106, 1824, 2127
PspPI GGNCC 1 cut(s) 1104
PspPPI RGGWCCY 1 cut(s) 1104
PstI CTGCAG 1 cut(s) 2293
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 3 cut(s) 987, 2125, 2425
RsaI GTAC 8 cut(s) 218, 740, 1029, 1078, 1949, 2149, 2168, 2473
RsaNI GTAC 8 cut(s) 217, 739, 1028, 1077, 1948, 2148, 2167, 2472
RseI CAYNNNNRTG 8 cut(s) 372, 932, 1124, 1170, 1770, 1776, 1955, 2079
SacI GAGCTC 2 cut(s) 554, 1691
SapI GCTCTTC 2 cut(s) 643, 1854
SaqAI TTAA 5 cut(s) 261, 321, 1272, 1484, 2118
SatI GCNGC 4 cut(s) 869, 1137, 1812, 1864
Sau96I GGNCC 1 cut(s) 1104
SchI GAGTC 5 cut(s) 1391, 1634, 2223, 2321, 2383
ScrFI CCNGG 6 cut(s) 143, 178, 605, 1265, 2200, 2244
SduI GDGCHC 6 cut(s) 554, 638, 956, 1010, 1564, 1691
SfaNI GCATC 4 cut(s) 232, 400, 448, 2229
SfcI CTRYAG 3 cut(s) 285, 1278, 2289
SfiI GGCCNNNNNGGCC 1 cut(s) 1980
SinI GGWCC 1 cut(s) 1104
SmiMI CAYNNNNRTG 8 cut(s) 372, 932, 1124, 1170, 1770, 1776, 1955, 2079
SmlI CTYRAG 2 cut(s) 1690, 2231
SmoI CTYRAG 2 cut(s) 1690, 2231
SnaBI TACGTA 1 cut(s) 1662
SpeI ACTAGT 1 cut(s) 1303
SseBI AGGCCT 1 cut(s) 326
SsiI CCGC 6 cut(s) 461, 767, 869, 875, 1249, 1811
SspI AATATT 1 cut(s) 2137
SspMI CTAG 7 cut(s) 357, 890, 1304, 1416, 1472, 1542, 2439
SstI GAGCTC 2 cut(s) 554, 1691
StuI AGGCCT 1 cut(s) 326
StyD4I CCNGG 6 cut(s) 141, 176, 603, 1263, 2198, 2242
TaaI ACNGT 7 cut(s) 94, 138, 820, 1531, 1537, 1947, 2147
TaiI ACGT 3 cut(s) 222, 1664, 2435
TaqI TCGA 2 cut(s) 164, 419
TatI WGTACW 4 cut(s) 738, 1076, 1947, 2471
TauI GCSGC 2 cut(s) 871, 1814
TfiI GAWTC 6 cut(s) 364, 670, 1111, 1183, 1223, 1322
Tru1I TTAA 5 cut(s) 261, 321, 1272, 1484, 2118
Tru9I TTAA 5 cut(s) 261, 321, 1272, 1484, 2118
TscAI CASTG 5 cut(s) 68, 825, 1027, 1348, 1542
TseFI GTSAC 1 cut(s) 1531
TseI GCWGC 2 cut(s) 1136, 1863
Tsp45I GTSAC 1 cut(s) 1531
TspRI CASTG 5 cut(s) 68, 825, 1027, 1348, 1542
VpaK11BI GGWCC 1 cut(s) 1104
XapI RAATTY 8 cut(s) 296, 995, 1147, 1290, 1619, 1723, 2070, 2161
XbaI TCTAGA 3 cut(s) 889, 1471, 2438
XceI RCATGY 3 cut(s) 377, 1057, 1504
XcmI CCANNNNNNNNNTGG 1 cut(s) 421
XspI CTAG 7 cut(s) 357, 890, 1304, 1416, 1472, 1542, 2439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.