Rorug07G0011000

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0014735
Physical Location & Seq
Forward (+)
905924 .. 909727
3804 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0011000.1

Sequence Viewer

Length: 1629 bp
ATGCCCATAATTCAAACCCACTCACCTTACTCTTTCAGCTCCTCCACTCTCAGACCCTCTTCCACATTCTTCACCGGAAAAGACCCAACTTTGAAACCCAATTTCGCTCCTTTCATCCGTACAAAACCTCCACGTTTGGTCCTCAGGGCCGCCGGAACCGATTACTATAAGACCCTCAACCTCGACAGAAATGCAACCCTGAAGGAGATAAAGACATCTTATCGAAAGCTCGCTCGACAGTACCATCCTGATTTGAACAAGGGCTCTGGAGCTGAAGACAAGTTCAAAGAGATTAGTGCTGCATATGAGGTCCTATCAGATGAGGAGAAAAGAGCTTCGTATGACCGCTTTGGCGAGGCAGGCTTACAGGGAGAATATGGTTCTTCGAACACGGGTTCAGCCGGGGTGGATCCTTTTGACGTTTTTGATGCGTACTTTGGGGGAAGGGGTGAATCAGGAGGCATGAACTTCAATTTTAACTTCAATATGCCAAACTCAGGAAATCAGGGCCTTGACATTCGGTATGAACTGCAACTGAGCTTTGAAGAATCCATTTTCGGAGGACAGAAGGAATTTGAAGTTTCTTGTGTTGAGACTTGTGATACATGTGGTGGAAGTGGTGCTAAATCTGATAACTGCATCAAATCATGTACTGGTTGTGGAGGAAGAGGAGGGGTGACAAAAACTCAGAGAACACCATTTGGCATGATATCCATGGTATCTACCTGCTCAAAGTGTGACGGTCAAGGTACAATAATAACTGATCCCTGCAAAAAATGCAATGGCAAGGGACAAGTGCAGTCAAAAAGAGCAATGGTTGTAACCATTCCAGCTGGTGTTAGTGATGGAGACACAATGAAAGTTCAAGGGGAGGGGAATATTGATAAGAGAAGGGGTGTAGTTGGTGATGTCTACATAATGCTTCGTATAAATGAAAAGGCTGGAATTCGGAGGGAGGGTGTCAATCTATATTCAACTATAAATATTGATTATACTGAGGCTATTTTAGGAACTGTGATAAAGGTAGAAACTGTGGAAGGCATGAAAGAGCTTGAAATTCCTTCTGGAATTCAGCCTGGAGAAACAGTAAAGTTGTCCCGTATGGGGGTTCCAAACATGCGTAAACCTTCTGTTAGAGGAGATCATCACTTTGTTGTGAATGTACTTATTCCAAAGAGAATCAGTGGCGCAGAACGCACACTTGTTGAAGAACTCGCTTCACTTAGGACCTCTAAAAAGGGCCACTCAGGTGCTTCAAAAGGCACTGGGACACAAGATGGGAATTCTGACGTGCATAAAAGCAGAAACCACGCAACTAGTAGTTCAAGGCAGGGGAAAAAATGTGTTTCGTCCTTGTGGAACTCTATAAAAGGCGTATTGGGGGGGAGGCAGTCGCAGGACAGATTTGCATCAGTTACCGTGGACAGACGGGCACTTTTGTGGGGTTCAAGCAAGCCTGATTCCTGTTATATGGCATCAATTTTTGGAGTTTTAGTTGTAACTTGTATTTGTACCGCGATAGGAAAGACCCGAAACCTCACTTTATCACAACACCACAAGGATAGTTCTCGTACTCATAGAACAAACAACAATGCTGAAGGAAATACTCAATCTGACATAGATGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

542

Amino Acids

58.84

Weight (kDa)

9.07

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 54 - 115 4.9e-25 DnaJ domain
DnaJ_C PF01556 173 - 391 4.7e-37 DnaJ C terminal domain
DnaJ_CXXCXGXG PF00684 200 - 264 1.5e-14 DnaJ central domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014735)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05260
malus_domestica MD06G1174400.v1.1 MD06G1174600.v1.1
prunus_persica Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1
pyrus_communis pycom06g15570
rosa_chinensis RchiOBHm_Chr7g0193611
rosa_laevigata RLG00000004256
rosa_multiflora Rmu_sc0042944.1_g000002
rosa_roxburghii Rroxscaffold_3G00261960
rosa_rugosa Rorug07G0011000 Rorug07G0011100
rosa_samantha Rh7AG137600 Rh7BG137800 Rh7CG141400
rosa_wichuraiana Rw7G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 734
AccI GTMKAC 1 cut(s) 912
AccII CGCG 1 cut(s) 1517
AciI CCGC 3 cut(s) 150, 346, 1515
AclWI GGATC 3 cut(s) 404, 417, 758
AcsI RAATTY 5 cut(s) 572, 945, 1056, 1068, 1282
AcuI CTGAAG 3 cut(s) 221, 294, 1617
AfaI GTAC 8 cut(s) 121, 242, 434, 652, 751, 1164, 1513, 1573
AfiI CCNNNNNNNGG 3 cut(s) 497, 1104, 1105
AflIII ACRYGT 1 cut(s) 605
AhlI ACTAGT 1 cut(s) 1316
AjiI CACGTC 1 cut(s) 1291
AjnI CCWGG 1 cut(s) 1075
AleI CACNNNNGTG 2 cut(s) 1248, 1438
AluBI AGCT 7 cut(s) 39, 229, 272, 335, 540, 833, 1051
AluI AGCT 7 cut(s) 39, 229, 272, 335, 540, 833, 1051
Alw26I GTCTC 2 cut(s) 587, 843
AlwI GGATC 3 cut(s) 404, 417, 758
AoxI GGCC 3 cut(s) 147, 508, 1240
ApeKI GCWGC 1 cut(s) 299
ApoI RAATTY 5 cut(s) 572, 945, 1056, 1068, 1282
AspLEI GCGC 1 cut(s) 1190
AspS9I GGNCC 6 cut(s) 139, 147, 310, 508, 1227, 1240
AsuC2I CCSGG 1 cut(s) 403
AsuHPI GGTGA 5 cut(s) 15, 64, 461, 688, 917
AsuII TTCGAA 1 cut(s) 386
AvaII GGWCC 3 cut(s) 139, 310, 1227
AxyI CCTNAGG 1 cut(s) 143
BaeGI GKGCMC 1 cut(s) 1435
BamHI GGATCC 1 cut(s) 409
BanII GRGCYC 1 cut(s) 266
BbsI GAAGAC 1 cut(s) 282
BbvI GCAGC 1 cut(s) 286
BccI CCATC 3 cut(s) 252, 839, 1271
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 1 cut(s) 1077
BcnI CCSGG 1 cut(s) 403
BcoDI GTCTC 2 cut(s) 587, 843
BcuI ACTAGT 1 cut(s) 1316
BfaI CTAG 1 cut(s) 1317
BfuAI ACCTGC 1 cut(s) 734
BisI GCNGC 2 cut(s) 150, 300
BlsI GCNGC 2 cut(s) 151, 301
Bme1390I CCNGG 2 cut(s) 403, 1077
Bme18I GGWCC 3 cut(s) 139, 310, 1227
BmgBI CACGTC 1 cut(s) 1291
BmgT120I GGNCC 6 cut(s) 139, 147, 310, 508, 1227, 1240
BmiI GGNNCC 3 cut(s) 157, 411, 1110
BmrFI CCNGG 2 cut(s) 403, 1077
BmrI ACTGGG 1 cut(s) 1275
BmsI GCATC 4 cut(s) 418, 648, 1418, 1484
BmuI ACTGGG 1 cut(s) 1275
BpiI GAAGAC 1 cut(s) 282
BpmI CTGGAG 2 cut(s) 288, 1098
Bpu14I TTCGAA 1 cut(s) 386
BpuMI CCSGG 1 cut(s) 403
BsaBI GATNNNNATC 1 cut(s) 1408
BsaJI CCNNGG 3 cut(s) 402, 714, 1419
BsaWI WCCGGW 1 cut(s) 74
BsaXI ACNNNNNCTCC 8 cut(s) 112, 142, 943, 973, 1071, 1101, 1479, 1509
Bsc4I CCNNNNNNNGG 3 cut(s) 497, 1104, 1105
Bse1I ACTGG 2 cut(s) 658, 1270
Bse21I CCTNAGG 1 cut(s) 143
Bse3DI GCAATG 2 cut(s) 787, 819
Bse8I GATNNNNATC 1 cut(s) 1408
BseBI CCWGG 1 cut(s) 1077
BseDI CCNNGG 3 cut(s) 402, 714, 1419
BseGI GGATG 2 cut(s) 114, 244
BseJI GATNNNNATC 1 cut(s) 1408
BseLI CCNNNNNNNGG 3 cut(s) 497, 1104, 1105
BseMI GCAATG 2 cut(s) 787, 819
BseMII CTCAG 7 cut(s) 64, 157, 510, 527, 701, 987, 1260
BseNI ACTGG 2 cut(s) 658, 1270
BseRI GAGGAG 4 cut(s) 31, 338, 684, 1152
BseSI GKGCMC 1 cut(s) 1435
BseXI GCAGC 1 cut(s) 286
BsgI GTGCAG 1 cut(s) 818
Bsh1236I CGCG 1 cut(s) 1517
BshFI GGCC 3 cut(s) 149, 510, 1242
BsiSI CCGG 3 cut(s) 75, 153, 402
BslFI GGGAC 3 cut(s) 804, 1081, 1282
BslI CCNNNNNNNGG 3 cut(s) 497, 1104, 1105
BsmAI GTCTC 2 cut(s) 587, 843
BsmFI GGGAC 3 cut(s) 804, 1081, 1282
BsnI GGCC 3 cut(s) 149, 510, 1242
Bsp119I TTCGAA 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 266, 1435
Bsp143I GATC 3 cut(s) 409, 763, 1141
Bsp19I CCATGG 1 cut(s) 714
BspACI CCGC 3 cut(s) 150, 346, 1515
BspANI GGCC 3 cut(s) 149, 510, 1242
BspCNI CTCAG 7 cut(s) 63, 156, 509, 528, 700, 988, 1259
BspFNI CGCG 1 cut(s) 1517
BspLI GGNNCC 3 cut(s) 157, 411, 1110
BspMI ACCTGC 1 cut(s) 734
BspPI GGATC 3 cut(s) 404, 417, 758
BspT104I TTCGAA 1 cut(s) 386
BsrDI GCAATG 2 cut(s) 787, 819
BsrI ACTGG 2 cut(s) 658, 1270
BssECI CCNNGG 3 cut(s) 402, 714, 1419
BssMI GATC 3 cut(s) 409, 763, 1141
BssT1I CCWWGG 1 cut(s) 714
Bst2UI CCWGG 1 cut(s) 1077
Bst4CI ACNGT 6 cut(s) 240, 743, 1015, 1033, 1087, 1420
Bst6I CTCTTC 2 cut(s) 64, 661
BstAPI GCANNNNNTGC 1 cut(s) 777
BstBI TTCGAA 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 231, 361, 1454
BstDEI CTNAG 8 cut(s) 50, 143, 496, 536, 687, 996, 1223, 1246
BstDSI CCRYGG 2 cut(s) 714, 1419
BstF5I GGATG 2 cut(s) 114, 244
BstFNI CGCG 1 cut(s) 1517
BstHHI GCGC 1 cut(s) 1190
BstKTI GATC 3 cut(s) 412, 766, 1144
BstMAI GTCTC 2 cut(s) 587, 843
BstMBI GATC 3 cut(s) 409, 763, 1141
BstMWI GCNNNNNNNGC 3 cut(s) 360, 777, 1194
BstNI CCWGG 1 cut(s) 1077
BstNSI RCATGY 2 cut(s) 609, 1120
BstSCI CCNGG 2 cut(s) 401, 1075
BstSLI GKGCMC 1 cut(s) 1435
BstUI CGCG 1 cut(s) 1517
BstV1I GCAGC 1 cut(s) 286
BstV2I GAAGAC 1 cut(s) 282
BstX2I RGATCY 1 cut(s) 409
BstYI RGATCY 1 cut(s) 409
Bsu36I CCTNAGG 1 cut(s) 143
BsuRI GGCC 3 cut(s) 149, 510, 1242
BtgI CCRYGG 2 cut(s) 714, 1419
BtrI CACGTC 1 cut(s) 1291
BtsCI GGATG 2 cut(s) 114, 244
BtsIMutI CAGTG 2 cut(s) 1189, 1263
BveI ACCTGC 1 cut(s) 734
Cac8I GCNNGC 3 cut(s) 231, 361, 1454
CfoI GCGC 1 cut(s) 1190
Cfr13I GGNCC 6 cut(s) 139, 147, 310, 508, 1227, 1240
Csp6I GTAC 8 cut(s) 120, 241, 433, 651, 750, 1163, 1512, 1572
CviAII CATG 7 cut(s) 463, 606, 648, 706, 715, 1042, 1117
CviQI GTAC 8 cut(s) 120, 241, 433, 651, 750, 1163, 1512, 1572
DdeI CTNAG 8 cut(s) 50, 143, 496, 536, 687, 996, 1223, 1246
DpnI GATC 3 cut(s) 411, 765, 1143
DpnII GATC 3 cut(s) 409, 763, 1141
Eam1104I CTCTTC 2 cut(s) 64, 661
EarI CTCTTC 2 cut(s) 64, 661
Eco130I CCWWGG 1 cut(s) 714
Eco24I GRGCYC 1 cut(s) 266
Eco32I GATATC 1 cut(s) 711
Eco47I GGWCC 3 cut(s) 139, 310, 1227
Eco57I CTGAAG 3 cut(s) 221, 294, 1617
Eco81I CCTNAGG 1 cut(s) 143
EcoO109I RGGNCCY 3 cut(s) 310, 508, 1227
EcoRI GAATTC 3 cut(s) 945, 1068, 1282
EcoRII CCWGG 1 cut(s) 1075
EcoRV GATATC 1 cut(s) 711
EcoT14I CCWWGG 1 cut(s) 714
EcoT38I GRGCYC 1 cut(s) 266
ErhI CCWWGG 1 cut(s) 714
FaeI CATG 7 cut(s) 466, 609, 651, 709, 718, 1045, 1120
FaqI GGGAC 3 cut(s) 804, 1081, 1282
FatI CATG 7 cut(s) 462, 605, 647, 705, 714, 1041, 1116
FauNDI CATATG 1 cut(s) 304
FblI GTMKAC 1 cut(s) 912
Fnu4HI GCNGC 2 cut(s) 150, 300
FokI GGATG 2 cut(s) 101, 231
FriOI GRGCYC 1 cut(s) 266
Fsp4HI GCNGC 2 cut(s) 150, 300
FspBI CTAG 1 cut(s) 1317
GlaI GCGC 1 cut(s) 1189
GluI GCNGC 2 cut(s) 150, 300
GsuI CTGGAG 2 cut(s) 288, 1098
HaeIII GGCC 3 cut(s) 149, 510, 1242
HapII CCGG 3 cut(s) 75, 153, 402
HhaI GCGC 1 cut(s) 1190
Hin1II CATG 7 cut(s) 466, 609, 651, 709, 718, 1045, 1120
Hin6I GCGC 1 cut(s) 1188
HinP1I GCGC 1 cut(s) 1188
HinfI GANTC 4 cut(s) 452, 548, 1179, 1460
HpaII CCGG 3 cut(s) 75, 153, 402
HphI GGTGA 5 cut(s) 15, 64, 461, 688, 917
Hpy166II GTNNAC 3 cut(s) 913, 1124, 1423
Hpy188I TCNGA 8 cut(s) 53, 319, 560, 631, 690, 951, 1288, 1615
Hpy188III TCNNGA 5 cut(s) 248, 267, 456, 498, 1065
Hpy8I GTNNAC 3 cut(s) 913, 1124, 1423
HpyAV CCTTC 8 cut(s) 196, 438, 562, 885, 1031, 1071, 1137, 1592
HpyCH4III ACNGT 6 cut(s) 240, 743, 1015, 1033, 1087, 1420
HpyCH4IV ACGT 3 cut(s) 133, 420, 1290
HpyCH4V TGCA 9 cut(s) 194, 302, 532, 639, 771, 780, 799, 1294, 1409
HpyF10VI GCNNNNNNNGC 3 cut(s) 360, 777, 1194
HpyF3I CTNAG 8 cut(s) 50, 143, 496, 536, 687, 996, 1223, 1246
HpySE526I ACGT 3 cut(s) 133, 420, 1290
Hsp92II CATG 7 cut(s) 466, 609, 651, 709, 718, 1045, 1120
HspAI GCGC 1 cut(s) 1188
Kzo9I GATC 3 cut(s) 409, 763, 1141
LmnI GCTCC 3 cut(s) 44, 112, 269
Lsp1109I GCAGC 1 cut(s) 286
LweI GCATC 4 cut(s) 418, 648, 1418, 1484
MaeI CTAG 1 cut(s) 1317
MaeII ACGT 3 cut(s) 133, 420, 1290
MaeIII GTNAC 5 cut(s) 676, 737, 820, 1414, 1498
MalI GATC 3 cut(s) 411, 765, 1143
MboI GATC 3 cut(s) 409, 763, 1141
MboII GAAGA 7 cut(s) 51, 61, 287, 375, 557, 678, 1220
MflI RGATCY 1 cut(s) 409
MhlI GDGCHC 2 cut(s) 266, 1435
MluCI AATT 9 cut(s) 9, 100, 472, 572, 945, 1056, 1068, 1282, 1479
MseI TTAA 2 cut(s) 477, 1627
MslI CAYNNNNRTG 2 cut(s) 1248, 1438
MspA1I CMGCKG 1 cut(s) 833
MspI CCGG 3 cut(s) 75, 153, 402
MspR9I CCNGG 2 cut(s) 403, 1077
MvaI CCWGG 1 cut(s) 1077
MvnI CGCG 1 cut(s) 1517
MwoI GCNNNNNNNGC 3 cut(s) 360, 777, 1194
NciI CCSGG 1 cut(s) 403
NcoI CCATGG 1 cut(s) 714
NdeI CATATG 1 cut(s) 304
NdeII GATC 3 cut(s) 409, 763, 1141
NlaIII CATG 7 cut(s) 466, 609, 651, 709, 718, 1045, 1120
NlaIV GGNNCC 3 cut(s) 157, 411, 1110
NmuCI GTSAC 2 cut(s) 676, 737
NspI RCATGY 2 cut(s) 609, 1120
NspV TTCGAA 1 cut(s) 386
OliI CACNNNNGTG 2 cut(s) 1248, 1438
PciI ACATGT 1 cut(s) 605
PfeI GAWTC 4 cut(s) 452, 548, 1179, 1460
PkrI GCNGC 2 cut(s) 151, 301
PpuMI RGGWCCY 2 cut(s) 310, 1227
PscI ACATGT 1 cut(s) 605
Psp5II RGGWCCY 2 cut(s) 310, 1227
Psp6I CCWGG 1 cut(s) 1075
PspGI CCWGG 1 cut(s) 1075
PspN4I GGNNCC 3 cut(s) 157, 411, 1110
PspPI GGNCC 6 cut(s) 139, 147, 310, 508, 1227, 1240
PspPPI RGGWCCY 2 cut(s) 310, 1227
PsuI RGATCY 1 cut(s) 409
PvuII CAGCTG 1 cut(s) 833
RsaI GTAC 8 cut(s) 121, 242, 434, 652, 751, 1164, 1513, 1573
RsaNI GTAC 8 cut(s) 120, 241, 433, 651, 750, 1163, 1512, 1572
RseI CAYNNNNRTG 2 cut(s) 1248, 1438
SaqAI TTAA 2 cut(s) 477, 1627
SatI GCNGC 2 cut(s) 150, 300
Sau3AI GATC 3 cut(s) 409, 763, 1141
Sau96I GGNCC 6 cut(s) 139, 147, 310, 508, 1227, 1240
ScrFI CCNGG 2 cut(s) 403, 1077
SduI GDGCHC 2 cut(s) 266, 1435
SfaNI GCATC 4 cut(s) 418, 648, 1418, 1484
SfuI TTCGAA 1 cut(s) 386
SinI GGWCC 3 cut(s) 139, 310, 1227
SmiMI CAYNNNNRTG 2 cut(s) 1248, 1438
SpeI ACTAGT 1 cut(s) 1316
Sse9I AATT 9 cut(s) 9, 100, 472, 572, 945, 1056, 1068, 1282, 1479
SsiI CCGC 3 cut(s) 150, 346, 1515
SspI AATATT 2 cut(s) 880, 985
SspMI CTAG 1 cut(s) 1317
StyD4I CCNGG 2 cut(s) 401, 1075
StyI CCWWGG 1 cut(s) 714
TaaI ACNGT 6 cut(s) 240, 743, 1015, 1033, 1087, 1420
TaiI ACGT 3 cut(s) 136, 423, 1293
TaqI TCGA 4 cut(s) 183, 223, 235, 386
TasI AATT 9 cut(s) 9, 100, 472, 572, 945, 1056, 1068, 1282, 1479
TatI WGTACW 2 cut(s) 650, 1162
TauI GCSGC 1 cut(s) 152
TfiI GAWTC 4 cut(s) 452, 548, 1179, 1460
Tru1I TTAA 2 cut(s) 477, 1627
Tru9I TTAA 2 cut(s) 477, 1627
TscAI CASTG 2 cut(s) 1189, 1270
TseFI GTSAC 2 cut(s) 676, 737
TseI GCWGC 1 cut(s) 299
Tsp45I GTSAC 2 cut(s) 676, 737
TspDTI ATGAA 6 cut(s) 103, 479, 540, 872, 948, 1058
TspGWI ACGGA 1 cut(s) 107
TspRI CASTG 2 cut(s) 1189, 1270
VpaK11BI GGWCC 3 cut(s) 139, 310, 1227
XapI RAATTY 5 cut(s) 572, 945, 1056, 1068, 1282
XceI RCATGY 2 cut(s) 609, 1120
XmiI GTMKAC 1 cut(s) 912
XspI CTAG 1 cut(s) 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.