RchiOBHm_Chr7g0193611

DYW family of nucleic acid deaminases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014735
Physical Location & Seq
Forward (+)
11901133 .. 11905286
4154 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17314

Sequence Viewer

Length: 2523 bp
ATGGCTGGTGTGAGAGTGAGACCACAAGGCTCGTGTTCGCTGAGACCATCGGAGCCATTACACTGCTACAAGTTGAGAGGTGGTGCTACAACAGTTTCTTTTGCTTCAAAGTTTCAATCTTTATGTTGCAAATCACAGTTCCCGGGAAACCCATCTCGGAAATCGAATTCCAGAGGTGGGTTTTCGAGGAATTGTTCCCGGATTGATGTTGTTTCGGCCGGTGAGCTGATCAAGAGGTACGTTGAGAATGGGAATTGCGAGGATGCGATAGTGGTTTATGTTAAAATGCTTGAACTTGGTTTGCCTGTAGCTGAGGAATTTCAGTTCTTTCCGATTTTGATTAAGGCCTTTGGGGGGCTTTGTGATGTGGGCAAGGCTAGGGAGATTCATGGGCATGTGTTGAAATTGGGGGTTTTGGGCGATGTTTACGATGCCAATTCGATTTTGGGGGTTTATTGGAAATGTGGGGAGGTTGACGATGCGGTTCAGATGTTTGAGAAAATGCCTGAGAGGGATTTGGTGTCTTGGAATACGATGATATCTGGGTTTTGCCATTCAGGGGATTATGTGAGCTCGTTGGGGATGTTTAGTCGGATGGTTCGGGATCATTGGGTGTTGCCTAATCGGGTGGCTTGCCTTTCGGCTCTCACTTCGTGCTCTTCGCTTCACTCTTTGGTTCATGGCAGAGAGATTCATGGCTTTGCCCTGAAGCGTTGGCTGGATGGTGATCAGTTTATGGTTAGTGGGTTGATTGATATGTACATGAAATGTGGGGATGTGAAGAATGCGGAACGTGTTTATAGGAGCGTTATTGGTGAAGCGTGTATTAGAGAAAATACAGTGATATGGAATGTGATGATTTCGGGCTATGTCTCTAATGGGTGTTGGCCGCAGGCCGCGGAGTTGTTTCTAGAGATGTTGGCGATTGGGTTCTTACCCGATACTTCCACAATGGTGGCCATTACAGGTTTGTGCTCTCACCTAATGGATTTAGCATTCGGAAAGCAGATCCATAAATTCTGTTTGAGCACTCAATTGAACACTGATGTACGAGTTGAAACAGCTCTTATGGACATGTATTTCAAATGTGGTGATAGCACAACTGGTCTTGAAATCTTCAGAAGGTCCCTGAATCGTAACATGGTCATGTGGGGCGCCGCCATCTCAAATTTTGCTCAAAGTAGTCATCCTCGTGAGGCATTGAATCTGTTTCATGACTATATTTTAGAATATGGCTTTGTGGATTCTGTCATAATCTTGGCTATTCTGCGGGCTTGTTCATCCCTGGCGGTTAAGTCTATAGGGATGGAAATTCATGGGCTACTAGTGAAATTGGGGTTTGATTCAGACCATTTTGTTGCCAGTGCATTAGTTGATTTTTATTCAAAAACCAGAGATATTGAGTCTGCTCAAAAAGTATTTCACAGACTACCAGCTAGAGATTTGGTGTCATGGAATGCCTTGATATCCGGATATACTCAGAATGAGTATCTAGATGAAGCTTTAAAGGCATTCCTTGACATGCAACTTGAAGAAATTAGACCTAATACTGTGACAGTGGCTAGTATTCTGTCAGTTTGTGCTCAGTTGTCAATCATGATGTTGTGTAAGGAGGTTCATGGTTACCTAATACGGCAAGAATTTGAGTCCAATGTTCTTGTGAGCAATTCCCTGATGACTACGTATGCAAAATGTGGAGACATAAAGAGCTCAAGGAGTGTGTTTGTGAAAATGCCTGAAAGAAATTTAGTTTCATGGAACTCAATTCTTCTGGGGTTAGGAGTGCATGGCCATGCAGATGAAACATTTGCTTTATTTGAAAAAATGGAAGCGGCAGGATTGGAGCCTGATCATGCAACTTTTACAGCTCTTCTTTCTGCTTGCAGCCATGCAGGGAGGATAGAAGAGGGGCTGAAGTATTTTCAGCGTATGGTAGAAGTTTACAAAATTGAGCCTCAACTTGAACAGTACACCTGCATGGTTGATCTTTTAGGCCGAGCTGGCCATCTAAAGCAAGCATATGATATTATTTCAACAATGCCTTGTGCCTCCGATGATCGTATATGGGGATCATTGCTTGGATCATGCAAAATTCATGGTGATGAAAGACTGGCAGAAGTTGTGGCTGATCATATCTTTAAACTGGATCCTACTAATATTGGCTACCGTACACTTCTTGCAAATTTGTACGAAGGTTATGGGAAATGGAATGACGTTACCAGGGTTAGATCAGACATCAAAGGTGTGGGACTCAAGAAGATGCCTGGGTGTAGTTGGATTGAAGTTGATAACAATCTCCATACCTTTACTGCAGGTGATCATTCTCATAACGAGGCAGAGGAGATATATTTTACAATAGAAAATCTGACAACTGAAATTAGGAAGGCAGGATATATCCCTCAATTACTGACTCTGGGTGAGGGGCTTGATGAGGTTCTTGATGAAGATCTTACGTCATCTAGAGACAAGGGTTTGTTGTTTCTGGTTGCAAGTACAAACAATCACCAACAAGTTTCAATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

840

Amino Acids

93.62

Weight (kDa)

5.86

Isoelectric Point (pI)

39.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR PF01535 174 - 202 9e-07 PPR repeat
PPR_2 PF13041 279 - 325 2.3e-07 PPR repeat family
PPR PF01535 281 - 311 1e-06 PPR repeat
PPR_2 PF13041 481 - 528 1.2e-09 PPR repeat family
PPR PF01535 483 - 510 4.2e-06 PPR repeat
PPR_2 PF13041 582 - 629 2.7e-10 PPR repeat family
PPR_long PF17177 599 - 680 6.6e-07 Pentacotripeptide-repeat region of PRORP
E_motif PF20431 699 - 761 2.4e-15 E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014735)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g05260
malus_domestica MD06G1174400.v1.1 MD06G1174600.v1.1
prunus_persica Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1 Prupe.5G174400_v2.0.a1
pyrus_communis pycom06g15570
rosa_chinensis RchiOBHm_Chr7g0193611
rosa_laevigata RLG00000004256
rosa_multiflora Rmu_sc0042944.1_g000002
rosa_roxburghii Rroxscaffold_3G00261960
rosa_rugosa Rorug07G0011000 Rorug07G0011100
rosa_samantha Rh7AG137600 Rh7BG137800 Rh7CG141400
rosa_wichuraiana Rw7G011720

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 1982, 2303
Acc36I ACCTGC 2 cut(s) 1982, 2303
AccB1I GGYRCC 1 cut(s) 1154
AccII CGCG 1 cut(s) 899
AccIII TCCGGA 1 cut(s) 1469
AciI CCGC 9 cut(s) 482, 788, 890, 897, 899, 1158, 1270, 1289, 1832
AclWI GGATC 6 cut(s) 612, 1003, 2077, 2089, 2141, 2154
AcoI YGGCCR 5 cut(s) 216, 887, 957, 1789, 2002
AcsI RAATTY 9 cut(s) 166, 317, 1016, 1168, 1311, 1640, 1744, 2091, 2182
AcuI CTGAAG 3 cut(s) 728, 1102, 1934
AcyI GRCGYC 1 cut(s) 1155
AdeI CACNNNGTG 1 cut(s) 654
AfaI GTAC 7 cut(s) 239, 761, 1050, 1970, 2170, 2189, 2494
AfiI CCNNNNNNNGG 3 cut(s) 177, 354, 559
AflIII ACRYGT 2 cut(s) 793, 1074
AhlI ACTAGT 1 cut(s) 1324
AjnI CCWGG 3 cut(s) 1284, 2219, 2263
AleI CACNNNNGTG 1 cut(s) 953
AloI GAACNNNNNNTCC 2 cut(s) 308, 340
AluBI AGCT 9 cut(s) 226, 311, 573, 1064, 1436, 1502, 1710, 1868, 2000
AluI AGCT 9 cut(s) 226, 311, 573, 1064, 1436, 1502, 1710, 1868, 2000
Alw21I GWGCWC 6 cut(s) 575, 659, 977, 1031, 1585, 1712
Alw26I GTCTC 5 cut(s) 13, 37, 877, 1692, 2457
AlwI GGATC 6 cut(s) 612, 1003, 2077, 2089, 2141, 2154
Ama87I CYCGRG 1 cut(s) 142
Aor13HI TCCGGA 1 cut(s) 1469
AoxI GGCC 8 cut(s) 216, 345, 887, 894, 957, 1789, 1993, 2002
ApeKI GCWGC 1 cut(s) 1884
ApoI RAATTY 9 cut(s) 166, 317, 1016, 1168, 1311, 1640, 1744, 2091, 2182
AspLEI GCGC 1 cut(s) 1157
AspS9I GGNCC 1 cut(s) 1125
AsuC2I CCSGG 3 cut(s) 143, 144, 199
AsuHPI GGTGA 9 cut(s) 233, 737, 827, 971, 1103, 2111, 2327, 2429, 2495
AvaI CYCGRG 1 cut(s) 142
AvaII GGWCC 1 cut(s) 1125
BalI TGGCCA 3 cut(s) 959, 1791, 2004
BamHI GGATCC 1 cut(s) 2146
BanI GGYRCC 1 cut(s) 1154
BanII GRGCYC 2 cut(s) 575, 1712
BauI CACGAG 2 cut(s) 31, 1191
Bbv12I GWGCWC 6 cut(s) 575, 659, 977, 1031, 1585, 1712
BbvCI CCTCAGC 1 cut(s) 312
BbvI GCAGC 1 cut(s) 1896
BccI CCATC 7 cut(s) 55, 160, 589, 716, 1169, 1300, 2013
BceAI ACGGC 1 cut(s) 1649
BciT130I CCWGG 3 cut(s) 1286, 2221, 2265
BclI TGATCA 5 cut(s) 228, 727, 1849, 2128, 2317
BcnI CCSGG 3 cut(s) 143, 144, 199
BcoDI GTCTC 5 cut(s) 13, 37, 877, 1692, 2457
BcuI ACTAGT 1 cut(s) 1324
BfaI CTAG 7 cut(s) 378, 911, 1325, 1437, 1493, 1563, 2460
BfmI CTRYAG 3 cut(s) 306, 1299, 2310
BfoI RGCGCY 1 cut(s) 1158
BfuAI ACCTGC 2 cut(s) 1982, 2303
BglI GCCNNNNNGGC 1 cut(s) 2001
BglII AGATCT 1 cut(s) 2446
BisI GCNGC 5 cut(s) 890, 897, 1158, 1833, 1885
BlsI GCNGC 5 cut(s) 891, 898, 1159, 1834, 1886
Bme1390I CCNGG 6 cut(s) 143, 144, 199, 1286, 2221, 2265
Bme18I GGWCC 1 cut(s) 1125
BmeT110I CYCGRG 1 cut(s) 142
BmgT120I GGNCC 1 cut(s) 1125
BmiI GGNNCC 5 cut(s) 54, 1127, 1156, 1845, 2148
BmrFI CCNGG 6 cut(s) 143, 144, 199, 1286, 2221, 2265
BmsI GCATC 4 cut(s) 253, 421, 469, 2250
Bpu10I CCTNAGC 1 cut(s) 312
BpuEI CTTGAG 2 cut(s) 1696, 2237
BpuMI CCSGG 3 cut(s) 143, 144, 199
BsaAI YACGTR 1 cut(s) 1683
BsaBI GATNNNNATC 3 cut(s) 726, 2292, 2445
BsaHI GRCGYC 1 cut(s) 1155
BsaI GGTCTC 2 cut(s) 13, 37
BsaJI CCNNGG 5 cut(s) 142, 897, 1284, 2220, 2264
BsaWI WCCGGW 1 cut(s) 1469
Bsc4I CCNNNNNNNGG 3 cut(s) 177, 354, 559
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 4 cut(s) 1108, 1362, 2115, 2148
Bse3DI GCAATG 1 cut(s) 2072
Bse8I GATNNNNATC 3 cut(s) 726, 2292, 2445
BseAI TCCGGA 1 cut(s) 1469
BseBI CCWGG 3 cut(s) 1286, 2221, 2265
BseDI CCNNGG 5 cut(s) 142, 897, 1284, 2220, 2264
BseGI GGATG 8 cut(s) 268, 588, 600, 727, 781, 1186, 1280, 1311
BseJI GATNNNNATC 3 cut(s) 726, 2292, 2445
BseLI CCNNNNNNNGG 3 cut(s) 177, 354, 559
BseMI GCAATG 1 cut(s) 2072
BseMII CTCAG 5 cut(s) 32, 303, 498, 1493, 1598
BseNI ACTGG 4 cut(s) 1108, 1362, 2115, 2148
BseRI GAGGAG 1 cut(s) 2354
BseX3I CGGCCG 1 cut(s) 216
BseXI GCAGC 1 cut(s) 1896
Bsh1236I CGCG 1 cut(s) 899
Bsh1285I CGRYCG 1 cut(s) 219
BshFI GGCC 8 cut(s) 218, 347, 889, 896, 959, 1791, 1995, 2004
BshNI GGYRCC 1 cut(s) 1154
BsiEI CGRYCG 1 cut(s) 219
BsiHKAI GWGCWC 6 cut(s) 575, 659, 977, 1031, 1585, 1712
BsiHKCI CYCGRG 1 cut(s) 142
BsiSI CCGG 4 cut(s) 143, 199, 219, 1470
BslFI GGGAC 2 cut(s) 1111, 2262
BslI CCNNNNNNNGG 3 cut(s) 177, 354, 559
BsmAI GTCTC 5 cut(s) 13, 37, 877, 1692, 2457
BsmFI GGGAC 2 cut(s) 1111, 2262
BsmI GAATGC 4 cut(s) 790, 995, 1462, 1511
BsnI GGCC 8 cut(s) 218, 347, 889, 896, 959, 1791, 1995, 2004
Bso31I GGTCTC 2 cut(s) 13, 37
BsoBI CYCGRG 1 cut(s) 142
Bsp1286I GDGCHC 6 cut(s) 575, 659, 977, 1031, 1585, 1712
Bsp13I TCCGGA 1 cut(s) 1469
Bsp1407I TGTACA 1 cut(s) 759
BspACI CCGC 9 cut(s) 482, 788, 890, 897, 899, 1158, 1270, 1289, 1832
BspANI GGCC 8 cut(s) 218, 347, 889, 896, 959, 1791, 1995, 2004
BspCNI CTCAG 5 cut(s) 33, 304, 499, 1492, 1597
BspEI TCCGGA 1 cut(s) 1469
BspFNI CGCG 1 cut(s) 899
BspHI TCATGA 2 cut(s) 1213, 1596
BspLI GGNNCC 5 cut(s) 54, 1127, 1156, 1845, 2148
BspMAI CTGCAG 1 cut(s) 2314
BspMI ACCTGC 2 cut(s) 1982, 2303
BspPI GGATC 6 cut(s) 612, 1003, 2077, 2089, 2141, 2154
BspQI GCTCTTC 2 cut(s) 664, 1875
BspT107I GGYRCC 1 cut(s) 1154
BspTNI GGTCTC 2 cut(s) 13, 37
BsrDI GCAATG 1 cut(s) 2072
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 759
BsrI ACTGG 4 cut(s) 1108, 1362, 2115, 2148
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 5 cut(s) 142, 897, 1284, 2220, 2264
BssNI GRCGYC 1 cut(s) 1155
BssSI CACGAG 2 cut(s) 31, 1191
Bst2BI CACGAG 2 cut(s) 31, 1191
Bst2UI CCWGG 3 cut(s) 1286, 2221, 2265
Bst4CI ACNGT 7 cut(s) 94, 138, 841, 1552, 1558, 1968, 2168
Bst6I CTCTTC 3 cut(s) 664, 1875, 1899
BstACI GRCGYC 1 cut(s) 1155
BstAUI TGTACA 1 cut(s) 759
BstBAI YACGTR 1 cut(s) 1683
BstC8I GCNNGC 6 cut(s) 634, 894, 1272, 1882, 2002, 2016
BstDEI CTNAG 5 cut(s) 41, 312, 507, 1479, 1584
BstDSI CCRYGG 1 cut(s) 897
BstEII GGTNACC 1 cut(s) 1622
BstF5I GGATG 8 cut(s) 268, 588, 600, 727, 781, 1186, 1280, 1311
BstFNI CGCG 1 cut(s) 899
BstH2I RGCGCY 1 cut(s) 1158
BstHHI GCGC 1 cut(s) 1157
BstMAI GTCTC 5 cut(s) 13, 37, 877, 1692, 2457
BstMCI CGRYCG 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 1508, 2001
BstNI CCWGG 3 cut(s) 1286, 2221, 2265
BstNSI RCATGY 3 cut(s) 398, 1078, 1525
BstPI GGTNACC 1 cut(s) 1622
BstSCI CCNGG 6 cut(s) 141, 142, 197, 1284, 2219, 2263
BstSFI CTRYAG 3 cut(s) 306, 1299, 2310
BstSNI TACGTA 1 cut(s) 1683
BstUI CGCG 1 cut(s) 899
BstV1I GCAGC 1 cut(s) 1896
BstX2I RGATCY 3 cut(s) 1008, 2146, 2446
BstXI CCANNNNNNTGG 1 cut(s) 955
BstYI RGATCY 3 cut(s) 1008, 2146, 2446
BstZI CGGCCG 1 cut(s) 216
BsuRI GGCC 8 cut(s) 218, 347, 889, 896, 959, 1791, 1995, 2004
BtgI CCRYGG 1 cut(s) 897
BtgZI GCGATG 1 cut(s) 435
BtsCI GGATG 8 cut(s) 268, 588, 600, 727, 781, 1186, 1280, 1311
BtsI GCAGTG 1 cut(s) 61
BtsIMutI CAGTG 5 cut(s) 61, 846, 1041, 1369, 1563
BveI ACCTGC 2 cut(s) 1982, 2303
Cac8I GCNNGC 6 cut(s) 634, 894, 1272, 1882, 2002, 2016
CciI TCATGA 2 cut(s) 1213, 1596
CfoI GCGC 1 cut(s) 1157
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 1125
Cfr42I CCGCGG 1 cut(s) 900
Cfr9I CCCGGG 1 cut(s) 142
Csp6I GTAC 7 cut(s) 238, 760, 1049, 1969, 2169, 2188, 2493
CviQI GTAC 7 cut(s) 238, 760, 1049, 1969, 2169, 2188, 2493
DdeI CTNAG 5 cut(s) 41, 312, 507, 1479, 1584
DinI GGCGCC 1 cut(s) 1156
DraI TTTAAA 2 cut(s) 1506, 2140
DraIII CACNNNGTG 1 cut(s) 654
EaeI YGGCCR 5 cut(s) 216, 887, 957, 1789, 2002
EagI CGGCCG 1 cut(s) 216
Eam1104I CTCTTC 3 cut(s) 664, 1875, 1899
EarI CTCTTC 3 cut(s) 664, 1875, 1899
Ecl136II GAGCTC 2 cut(s) 573, 1710
EclXI CGGCCG 1 cut(s) 216
Eco105I TACGTA 1 cut(s) 1683
Eco147I AGGCCT 1 cut(s) 347
Eco24I GRGCYC 2 cut(s) 575, 1712
Eco31I GGTCTC 2 cut(s) 13, 37
Eco32I GATATC 2 cut(s) 540, 1467
Eco47I GGWCC 1 cut(s) 1125
Eco52I CGGCCG 1 cut(s) 216
Eco53kI GAGCTC 2 cut(s) 573, 1710
Eco57I CTGAAG 3 cut(s) 728, 1102, 1934
Eco88I CYCGRG 1 cut(s) 142
Eco91I GGTNACC 1 cut(s) 1622
EcoICRI GAGCTC 2 cut(s) 573, 1710
EcoO109I RGGNCCY 1 cut(s) 1125
EcoO65I GGTNACC 1 cut(s) 1622
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 3 cut(s) 1284, 2219, 2263
EcoRV GATATC 2 cut(s) 540, 1467
EcoT38I GRGCYC 2 cut(s) 575, 1712
EgeI GGCGCC 1 cut(s) 1156
EheI GGCGCC 1 cut(s) 1156
FaqI GGGAC 2 cut(s) 1111, 2262
FauI CCCGC 1 cut(s) 1263
FauNDI CATATG 1 cut(s) 2020
FbaI TGATCA 5 cut(s) 228, 727, 1849, 2128, 2317
Fnu4HI GCNGC 5 cut(s) 890, 897, 1158, 1833, 1885
FokI GGATG 8 cut(s) 275, 595, 607, 734, 788, 1173, 1267, 1318
FriOI GRGCYC 2 cut(s) 575, 1712
Fsp4HI GCNGC 5 cut(s) 890, 897, 1158, 1833, 1885
FspBI CTAG 7 cut(s) 378, 911, 1325, 1437, 1493, 1563, 2460
GlaI GCGC 1 cut(s) 1156
GluI GCNGC 5 cut(s) 890, 897, 1158, 1833, 1885
HaeII RGCGCY 1 cut(s) 1158
HaeIII GGCC 8 cut(s) 218, 347, 889, 896, 959, 1791, 1995, 2004
HapII CCGG 4 cut(s) 143, 199, 219, 1470
HhaI GCGC 1 cut(s) 1157
Hin1I GRCGYC 1 cut(s) 1155
Hin6I GCGC 1 cut(s) 1155
HinP1I GCGC 1 cut(s) 1155
HincII GTYRAC 1 cut(s) 475
HindII GTYRAC 1 cut(s) 475
HindIII AAGCTT 1 cut(s) 1500
HpaII CCGG 4 cut(s) 143, 199, 219, 1470
HphI GGTGA 9 cut(s) 233, 737, 827, 971, 1103, 2111, 2327, 2429, 2495
Hpy166II GTNNAC 5 cut(s) 427, 475, 1942, 1971, 2171
Hpy8I GTNNAC 5 cut(s) 427, 475, 1942, 1971, 2171
HpyAV CCTTC 3 cut(s) 1116, 2186, 2377
HpyCH4III ACNGT 7 cut(s) 94, 138, 841, 1552, 1558, 1968, 2168
HpyCH4IV ACGT 5 cut(s) 240, 793, 1682, 2214, 2453
HpyF10VI GCNNNNNNNGC 2 cut(s) 1508, 2001
HpyF3I CTNAG 5 cut(s) 41, 312, 507, 1479, 1584
HpySE526I ACGT 5 cut(s) 240, 793, 1682, 2214, 2453
Hsp92I GRCGYC 1 cut(s) 1155
HspAI GCGC 1 cut(s) 1155
KasI GGCGCC 1 cut(s) 1154
Kpn2I TCCGGA 1 cut(s) 1469
Ksp22I TGATCA 5 cut(s) 228, 727, 1849, 2128, 2317
KspI CCGCGG 1 cut(s) 900
LguI GCTCTTC 2 cut(s) 664, 1875
LmnI GCTCC 3 cut(s) 52, 804, 1843
Lsp1109I GCAGC 1 cut(s) 1896
LweI GCATC 4 cut(s) 253, 421, 469, 2250
MaeI CTAG 7 cut(s) 378, 911, 1325, 1437, 1493, 1563, 2460
MaeII ACGT 5 cut(s) 240, 793, 1682, 2214, 2453
MaeIII GTNAC 4 cut(s) 1136, 1552, 1622, 2215
MboII GAAGA 9 cut(s) 651, 793, 1108, 1544, 1760, 1862, 1916, 2269, 2456
MfeI CAATTG 1 cut(s) 1034
MflI RGATCY 3 cut(s) 1008, 2146, 2446
MhlI GDGCHC 6 cut(s) 575, 659, 977, 1031, 1585, 1712
MlsI TGGCCA 3 cut(s) 959, 1791, 2004
MluNI TGGCCA 3 cut(s) 959, 1791, 2004
Mly113I GGCGCC 1 cut(s) 1155
MlyI GAGTC 4 cut(s) 1412, 1655, 2244, 2404
MmeI TCCRAC 2 cut(s) 572, 2255
Mox20I TGGCCA 3 cut(s) 959, 1791, 2004
MroI TCCGGA 1 cut(s) 1469
MscI TGGCCA 3 cut(s) 959, 1791, 2004
MseI TTAA 5 cut(s) 282, 342, 1293, 1505, 2139
MslI CAYNNNNRTG 8 cut(s) 393, 953, 1145, 1191, 1791, 1797, 1976, 2100
Msp20I TGGCCA 3 cut(s) 959, 1791, 2004
MspA1I CMGCKG 1 cut(s) 899
MspI CCGG 4 cut(s) 143, 199, 219, 1470
MspR9I CCNGG 6 cut(s) 143, 144, 199, 1286, 2221, 2265
MunI CAATTG 1 cut(s) 1034
Mva1269I GAATGC 4 cut(s) 790, 995, 1462, 1511
MvaI CCWGG 3 cut(s) 1286, 2221, 2265
MvnI CGCG 1 cut(s) 899
MwoI GCNNNNNNNGC 2 cut(s) 1508, 2001
NarI GGCGCC 1 cut(s) 1155
NciI CCSGG 3 cut(s) 143, 144, 199
NdeI CATATG 1 cut(s) 2020
NlaIV GGNNCC 5 cut(s) 54, 1127, 1156, 1845, 2148
NmeAIII GCCGAG 1 cut(s) 2021
NmuCI GTSAC 1 cut(s) 1552
NspI RCATGY 3 cut(s) 398, 1078, 1525
OliI CACNNNNGTG 1 cut(s) 953
PagI TCATGA 2 cut(s) 1213, 1596
PaqCI CACCTGC 2 cut(s) 1982, 2303
PceI AGGCCT 1 cut(s) 347
PciI ACATGT 1 cut(s) 1074
PciSI GCTCTTC 2 cut(s) 664, 1875
PctI GAATGC 4 cut(s) 790, 995, 1462, 1511
PfeI GAWTC 6 cut(s) 385, 691, 1132, 1204, 1244, 1343
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 5 cut(s) 891, 898, 1159, 1834, 1886
PleI GAGTC 4 cut(s) 1411, 1654, 2244, 2404
PluTI GGCGCC 1 cut(s) 1158
PpsI GAGTC 4 cut(s) 1411, 1654, 2244, 2404
Ppu21I YACGTR 1 cut(s) 1683
PpuMI RGGWCCY 1 cut(s) 1125
PscI ACATGT 1 cut(s) 1074
Psp124BI GAGCTC 2 cut(s) 575, 1712
Psp5II RGGWCCY 1 cut(s) 1125
Psp6I CCWGG 3 cut(s) 1284, 2219, 2263
PspEI GGTNACC 1 cut(s) 1622
PspGI CCWGG 3 cut(s) 1284, 2219, 2263
PspN4I GGNNCC 5 cut(s) 54, 1127, 1156, 1845, 2148
PspPI GGNCC 1 cut(s) 1125
PspPPI RGGWCCY 1 cut(s) 1125
PstI CTGCAG 1 cut(s) 2314
PsuI RGATCY 3 cut(s) 1008, 2146, 2446
RsaI GTAC 7 cut(s) 239, 761, 1050, 1970, 2170, 2189, 2494
RsaNI GTAC 7 cut(s) 238, 760, 1049, 1969, 2169, 2188, 2493
RseI CAYNNNNRTG 8 cut(s) 393, 953, 1145, 1191, 1791, 1797, 1976, 2100
SacI GAGCTC 2 cut(s) 575, 1712
SacII CCGCGG 1 cut(s) 900
SapI GCTCTTC 2 cut(s) 664, 1875
SaqAI TTAA 5 cut(s) 282, 342, 1293, 1505, 2139
SatI GCNGC 5 cut(s) 890, 897, 1158, 1833, 1885
Sau96I GGNCC 1 cut(s) 1125
SchI GAGTC 4 cut(s) 1412, 1655, 2244, 2404
ScrFI CCNGG 6 cut(s) 143, 144, 199, 1286, 2221, 2265
SduI GDGCHC 6 cut(s) 575, 659, 977, 1031, 1585, 1712
SfaNI GCATC 4 cut(s) 253, 421, 469, 2250
SfcI CTRYAG 3 cut(s) 306, 1299, 2310
SfiI GGCCNNNNNGGCC 1 cut(s) 2001
SfoI GGCGCC 1 cut(s) 1156
Sfr303I CCGCGG 1 cut(s) 900
SgrBI CCGCGG 1 cut(s) 900
SinI GGWCC 1 cut(s) 1125
SmaI CCCGGG 1 cut(s) 144
SmiMI CAYNNNNRTG 8 cut(s) 393, 953, 1145, 1191, 1791, 1797, 1976, 2100
SmlI CTYRAG 2 cut(s) 1711, 2252
SmoI CTYRAG 2 cut(s) 1711, 2252
SnaBI TACGTA 1 cut(s) 1683
SpeI ACTAGT 1 cut(s) 1324
SseBI AGGCCT 1 cut(s) 347
SsiI CCGC 9 cut(s) 482, 788, 890, 897, 899, 1158, 1270, 1289, 1832
SspDI GGCGCC 1 cut(s) 1154
SspI AATATT 1 cut(s) 2158
SspMI CTAG 7 cut(s) 378, 911, 1325, 1437, 1493, 1563, 2460
SstI GAGCTC 2 cut(s) 575, 1712
StuI AGGCCT 1 cut(s) 347
StyD4I CCNGG 6 cut(s) 141, 142, 197, 1284, 2219, 2263
TaaI ACNGT 7 cut(s) 94, 138, 841, 1552, 1558, 1968, 2168
TaiI ACGT 5 cut(s) 243, 796, 1685, 2217, 2456
TaqI TCGA 3 cut(s) 164, 185, 440
TatI WGTACW 3 cut(s) 759, 1968, 2492
TauI GCSGC 4 cut(s) 892, 899, 1160, 1835
TfiI GAWTC 6 cut(s) 385, 691, 1132, 1204, 1244, 1343
Tru1I TTAA 5 cut(s) 282, 342, 1293, 1505, 2139
Tru9I TTAA 5 cut(s) 282, 342, 1293, 1505, 2139
TscAI CASTG 5 cut(s) 68, 846, 1048, 1369, 1563
TseFI GTSAC 1 cut(s) 1552
TseI GCWGC 1 cut(s) 1884
Tsp45I GTSAC 1 cut(s) 1552
TspMI CCCGGG 1 cut(s) 142
TspRI CASTG 5 cut(s) 68, 846, 1048, 1369, 1563
VpaK11BI GGWCC 1 cut(s) 1125
XapI RAATTY 9 cut(s) 166, 317, 1016, 1168, 1311, 1640, 1744, 2091, 2182
XbaI TCTAGA 3 cut(s) 910, 1492, 2459
XceI RCATGY 3 cut(s) 398, 1078, 1525
XcmI CCANNNNNNNNNTGG 1 cut(s) 442
XmaI CCCGGG 1 cut(s) 142
XspI CTAG 7 cut(s) 378, 911, 1325, 1437, 1493, 1563, 2460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.