Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0010667

Physical Location & Seq

Genomic Coordinates Reverse (-)

31706144 .. 31710131

Gene Structure 3988 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1177300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2097 bp

ATGATGCACAGCTTCCAGCCATATGCCGCTGCCTCGGCTCGCTTCCACAACTTCTCGACCCCATTTTACCTCAACAAGAAGATCCCGTGGTGGTACCAGATCCTCGACCCAGGATCCGAACGCGTGACCCAATGGAACCACATCTTCTTTGTCATATGCATCGCCGGCCTTTTCCTTGACCCCCTCTATTTCTTCTTGCCCACCACGTCCAGCGACCAAGCCGCTTGCATGCAGATTGACATGGGCCTTGGCATCTTGGTCACCTTCCTCCGTACAGTCGCTGACTTCTTCTACATTATCCATATCCTAATCAAATTTCGAATTGCTTATATCGACCCTGGTTCTAGGGTTTTTGGGCGGGGCCAGCTCGTTAAAGACCCCAGGGCCATCGCGATCCGCTACCTCAAGGGCGACTTCTCTATCGATCTCGCCGCCTCCCTACCACTCCCACAGATTATGATTTGGTTTGTAATTCCCTCAGTGAAAAACTCAACAGCTGCTCATGCTAATCACACCCTTTCTCTAATTGTGCTCATTCAATATATTCCTCGATTTATTCAACTTTTCCCTCTCAACCGGCAAATTACTAAATCGACCGGCGTTGTAGCCAAGACTGCTTGGTCAGGGGCAGCTTACAATCTTGTTCTGTTTTGTCTAATTGCACATATTGTTGGAGCTTCATGGTATGTGCTGTCCATTAGACGGCAGTACGAATGCTGGACGCAAGAATGTAAAAAGGAAGTGAATAACACACACTCTCCATCCTGTAAAATTTCGTTTCTTGATTGTGCCAATGCCGAAAACCGCGAACGAAAACTTTGGCTAAAAATCACAAAAGTTGAGACTAGTTGTGATGCTGCTGGTAAGGACGACTTTGAGTTTGGAATGTTTGCGGAGGCTTTAACTAACGAGGTTGCTTCTGCATACATCATTGAAAAATATTTCTACTGCCTTTGGTGGGGTATGAGAAATTTATGTTCATACGGACAAGATATTAAGCCAAGCACGTTTATCAGTGAAACCTCATTTTGCATTCTCATTGCCGTTCTTGGTCTAGTTCTCTTCTCACATCTCATGAGCCAGATGCAGACCTATCTGCAATCTGCAACGATTAGAATTGAGGAATGGCGGGTAAAAAGGAGAGACACAGAGGAGTGGATGAGGCACCGTCAGCTCCCTCCGGAGCTGCAAGAGCGTGTTCGTCGGTTTGTTCAATATAAGTGGATTGCCACAAGAGGTGTAGATGAGAAAAACATTTTGCAATCCTTGCCTGTGGATCTCCGCCGCCAAATCCAAAGGCATTTATGTCTTGCCCTTGTTCGCCGTGTACCCTTTTTCGCACAAATGGACGATCAGCTTCTAGACGCCATATGTGAACGCCTTGAGTCATCCCTAAATACCAGGAACACATACATCATCAGGGAAGGTGATCCTGTGAATGAGATGCTTTTCATCATCAGAGGACAACTTGAGAGCTCCACAACTGATGGTGGAAGGACAGGGTTCTACAACTCAACTACCCTCAAGCGGGGGGATTTCTGCGGCGAAGAGTTGCTCACATGGGCCTTGATGCCTACATCAAGCCTCAACCTTCCATGCTCGACACGCACAGTGAGGTCCCTAACTGAAGTTGAGGCATTTGCACTAAAAGCTGAAGACCTCAAGTACGTTGCCAATCAATTCAAGCGCCTGCACAGCAAAAAACTGCAGCATGCTTTTCGGTACTATTCACACCAGTGGAGAACTTGGGGGGCTTGTTTTATACAAGTTGCTTGGAGAAGGTTTAGGAAGCGAAAGTTGGCAATGGAGTTGGCAAAAGAGGAGGAGTACTATTATACACATGTTTTGGAGCAAGAAGAGCATGGCAATGGCAATGAATCAAGAGTATGGACTAGTAGCATAGGTGGTGCTGAGAGGTCATCGTCAGAGGCGAACTTGCCGCATAGTATTCCGCTTGGGCCGGCGTTGTTGGCTTCCAAATTTGCTGCAACCACCAGAAGAGGGATTCAGAAGGTGGCATCAGTTATTCACCATGATGAAACCAGTATGCCCAAAAAACTGTTCAAGCCAGATGAGCCTGATTTTTCAGCAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

699

Amino Acids

80.58

Weight (kDa)

8.98

Isoelectric Point (pI)

46.72

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	46 - 370	5.8e-14	Ion transport protein
cNMP_binding	PF00027	468 - 558	4.8e-08	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0010667)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48010
fragaria_vesca	FvH4_5g05660
malus_domestica	MD06G1177300.v1.1 MD14G1183500.v1.1
prunus_persica	Prupe.5G176000_v2.0.a1 Prupe.5G176000_v2.0.a1
pyrus_communis	pycom14g15260
rosa_chinensis	RchiOBHm_Chr7g0193151
rosa_laevigata	RLG00000004292
rosa_multiflora	Rmu_co8354259.1_g000001 Rmu_co8396983.1_g000001 Rmu_ssc0000092.1_g000037
rosa_roxburghii	Rroxscaffold_3G00262340
rosa_rugosa	Rorug07G0008000 Rorug07G0008100
rosa_samantha	Rh7AG133100 Rh7BG134200 Rh7CG137000 Rh7DG135000
rosa_wichuraiana	Rw7G011380

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	619
Acc65I	GGTACC	1 cut(s)	93
AccB1I	GGYRCC	2 cut(s)	93, 1164
AccII	CGCG	3 cut(s)	123, 392, 807
AccIII	TCCGGA	1 cut(s)	1180
AclWI	GGATC	7 cut(s)	76, 94, 108, 121, 388, 1284, 1424
AcsI	RAATTY	4 cut(s)	314, 771, 970, 1979
AcuI	CTGAAG	2 cut(s)	1647, 1674
AcyI	GRCGYC	1 cut(s)	1365
AfaI	GTAC	7 cut(s)	95, 274, 710, 1329, 1667, 1724, 1829
AfiI	CCNNNNNNNGG	5 cut(s)	702, 958, 1527, 1528, 2001
AflIII	ACRYGT	2 cut(s)	121, 1840
AgsI	TTSAA	6 cut(s)	539, 560, 935, 1214, 1684, 2065
AhlI	ACTAGT	2 cut(s)	845, 1892
AjiI	CACGTC	1 cut(s)	207
AjnI	CCWGG	4 cut(s)	109, 337, 380, 1400
AjuI	GAANNNNNNNTTGG	4 cut(s)	1781, 1813, 2045, 2077
AleI	CACNNNNGTG	1 cut(s)	1735
Alw21I	GWGCWC	2 cut(s)	534, 1478
Alw26I	GTCTC	2 cut(s)	836, 1137
AlwI	GGATC	7 cut(s)	76, 94, 108, 121, 388, 1284, 1424
AlwNI	CAGNNNCTG	1 cut(s)	281
Aor13HI	TCCGGA	1 cut(s)	1180
AoxI	GGCC	6 cut(s)	166, 244, 361, 384, 1563, 1958
ApeKI	GCWGC	7 cut(s)	29, 497, 629, 857, 1186, 1708, 1985
ApoI	RAATTY	4 cut(s)	314, 771, 970, 1979
ArsI	GACNNNNNNTTYG	2 cut(s)	863, 895
Asp718I	GGTACC	1 cut(s)	93
AspLEI	GCGC	1 cut(s)	1689
AspS9I	GGNCC	6 cut(s)	244, 361, 384, 1563, 1617, 1958
AsuHPI	GGTGA	3 cut(s)	253, 1439, 2021
AsuII	TTCGAA	1 cut(s)	319
AvaII	GGWCC	1 cut(s)	1617
BamHI	GGATCC	1 cut(s)	113
BanI	GGYRCC	2 cut(s)	93, 1164
BanII	GRGCYC	1 cut(s)	1478
BbsI	GAAGAC	1 cut(s)	1662
Bbv12I	GWGCWC	2 cut(s)	534, 1478
BbvI	GCAGC	7 cut(s)	16, 484, 641, 844, 1173, 1720, 1972
BccI	CCATC	3 cut(s)	395, 769, 1481
BceAI	ACGGC	3 cut(s)	719, 1028, 1308
BcgI	CGANNNNNNTGC	2 cut(s)	1700, 1734
BciT130I	CCWGG	4 cut(s)	111, 339, 382, 1402
BcoDI	GTCTC	2 cut(s)	836, 1137
BcuI	ACTAGT	2 cut(s)	845, 1892
BfaI	CTAG	5 cut(s)	345, 846, 1055, 1361, 1893
BfmI	CTRYAG	1 cut(s)	1706
BfoI	RGCGCY	1 cut(s)	1690
BmcAI	AGTACT	1 cut(s)	1829
Bme1390I	CCNGG	4 cut(s)	111, 339, 382, 1402
Bme18I	GGWCC	1 cut(s)	1617
BmgBI	CACGTC	1 cut(s)	207
BmgT120I	GGNCC	6 cut(s)	244, 361, 384, 1563, 1617, 1958
BmiI	GGNNCC	6 cut(s)	95, 115, 137, 362, 1166, 1619
BmrFI	CCNGG	4 cut(s)	111, 339, 382, 1402
BmsI	GCATC	7 cut(s)	168, 261, 844, 1074, 1434, 1560, 2027
BpiI	GAAGAC	1 cut(s)	1662
Bpu14I	TTCGAA	1 cut(s)	319
BpuEI	CTTGAG	5 cut(s)	389, 1403, 1490, 1508, 1646
Bsa29I	ATCGAT	1 cut(s)	423
BsaHI	GRCGYC	1 cut(s)	1365
BsaJI	CCNNGG	7 cut(s)	33, 86, 109, 247, 337, 380, 381
BsaWI	WCCGGW	1 cut(s)	1180
BsaXI	ACNNNNNCTCC	2 cut(s)	742, 772
Bsc4I	CCNNNNNNNGG	5 cut(s)	702, 958, 1527, 1528, 2001
Bse118I	RCCGGY	4 cut(s)	164, 576, 596, 1960
Bse1I	ACTGG	2 cut(s)	1735, 2043
Bse3DI	GCAATG	4 cut(s)	1038, 1809, 1873, 1879
BseAI	TCCGGA	1 cut(s)	1180
BseBI	CCWGG	4 cut(s)	111, 339, 382, 1402
BseCI	ATCGAT	1 cut(s)	423
BseDI	CCNNGG	7 cut(s)	33, 86, 109, 247, 337, 380, 381
BseGI	GGATG	3 cut(s)	761, 1164, 1388
BseLI	CCNNNNNNNGG	5 cut(s)	702, 958, 1527, 1528, 2001
BseMI	GCAATG	4 cut(s)	1038, 1809, 1873, 1879
BseMII	CTCAG	2 cut(s)	492, 1902
BseNI	ACTGG	2 cut(s)	1735, 2043
BseRI	GAGGAG	3 cut(s)	1166, 1835, 1838
BseXI	GCAGC	7 cut(s)	16, 484, 641, 844, 1173, 1720, 1972
BsgI	GTGCAG	1 cut(s)	1676
Bsh1236I	CGCG	3 cut(s)	123, 392, 807
Bsh1285I	CGRYCG	1 cut(s)	597
BshFI	GGCC	6 cut(s)	168, 246, 363, 386, 1565, 1960
BshNI	GGYRCC	2 cut(s)	93, 1164
BshVI	ATCGAT	1 cut(s)	423
BsiEI	CGRYCG	1 cut(s)	597
BsiHKAI	GWGCWC	2 cut(s)	534, 1478
BsiSI	CCGG	5 cut(s)	165, 577, 597, 1181, 1961
BslFI	GGGAC	1 cut(s)	1603
BslI	CCNNNNNNNGG	5 cut(s)	702, 958, 1527, 1528, 2001
BsmAI	GTCTC	2 cut(s)	836, 1137
BsmFI	GGGAC	1 cut(s)	1603
BsmI	GAATGC	2 cut(s)	719, 1032
BsnI	GGCC	6 cut(s)	168, 246, 363, 386, 1565, 1960
Bsp119I	TTCGAA	1 cut(s)	319
Bsp1286I	GDGCHC	2 cut(s)	534, 1478
Bsp13I	TCCGGA	1 cut(s)	1180
Bsp143I	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
Bsp68I	TCGCGA	1 cut(s)	392
BspANI	GGCC	6 cut(s)	168, 246, 363, 386, 1565, 1960
BspCNI	CTCAG	2 cut(s)	491, 1903
BspDI	ATCGAT	1 cut(s)	423
BspEI	TCCGGA	1 cut(s)	1180
BspFNI	CGCG	3 cut(s)	123, 392, 807
BspHI	TCATGA	1 cut(s)	1074
BspLI	GGNNCC	6 cut(s)	95, 115, 137, 362, 1166, 1619
BspMAI	CTGCAG	1 cut(s)	1710
BspPI	GGATC	7 cut(s)	76, 94, 108, 121, 388, 1284, 1424
BspQI	GCTCTTC	1 cut(s)	1851
BspT104I	TTCGAA	1 cut(s)	319
BspT107I	GGYRCC	2 cut(s)	93, 1164
BsrDI	GCAATG	4 cut(s)	1038, 1809, 1873, 1879
BsrFI	RCCGGY	4 cut(s)	164, 576, 596, 1960
BsrI	ACTGG	2 cut(s)	1735, 2043
BssAI	RCCGGY	4 cut(s)	164, 576, 596, 1960
BssECI	CCNNGG	7 cut(s)	33, 86, 109, 247, 337, 380, 381
BssMI	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
BssNI	GRCGYC	1 cut(s)	1365
BssT1I	CCWWGG	1 cut(s)	247
Bst2UI	CCWGG	4 cut(s)	111, 339, 382, 1402
Bst4CI	ACNGT	4 cut(s)	277, 1169, 1612, 2061
Bst6I	CTCTTC	4 cut(s)	1067, 1542, 1851, 1993
BstACI	GRCGYC	1 cut(s)	1365
BstAPI	GCANNNNNTGC	1 cut(s)	1267
BstBI	TTCGAA	1 cut(s)	319
BstC8I	GCNNGC	8 cut(s)	40, 166, 226, 230, 365, 1691, 1713, 1962
BstDEI	CTNAG	2 cut(s)	478, 1911
BstDSI	CCRYGG	1 cut(s)	86
BstEII	GGTNACC	1 cut(s)	259
BstF5I	GGATG	3 cut(s)	761, 1164, 1388
BstFNI	CGCG	3 cut(s)	123, 392, 807
BstH2I	RGCGCY	1 cut(s)	1690
BstHHI	GCGC	1 cut(s)	1689
BstKTI	GATC	8 cut(s)	84, 102, 116, 396, 427, 1279, 1354, 1432
BstMAI	GTCTC	2 cut(s)	836, 1137
BstMBI	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
BstMCI	CGRYCG	1 cut(s)	597
BstNI	CCWGG	4 cut(s)	111, 339, 382, 1402
BstNSI	RCATGY	3 cut(s)	232, 1715, 1844
BstPI	GGTNACC	1 cut(s)	259
BstSCI	CCNGG	4 cut(s)	109, 337, 380, 1400
BstSFI	CTRYAG	1 cut(s)	1706
BstUI	CGCG	3 cut(s)	123, 392, 807
BstV1I	GCAGC	7 cut(s)	16, 484, 641, 844, 1173, 1720, 1972
BstV2I	GAAGAC	1 cut(s)	1662
BstX2I	RGATCY	4 cut(s)	81, 99, 113, 1276
BstYI	RGATCY	4 cut(s)	81, 99, 113, 1276
Bsu15I	ATCGAT	1 cut(s)	423
BsuRI	GGCC	6 cut(s)	168, 246, 363, 386, 1565, 1960
BsuTUI	ATCGAT	1 cut(s)	423
BtgI	CCRYGG	1 cut(s)	86
BtgZI	GCGATG	2 cut(s)	145, 373
BtrI	CACGTC	1 cut(s)	207
BtsCI	GGATG	3 cut(s)	761, 1164, 1388
BtsIMutI	CAGTG	4 cut(s)	486, 1021, 1617, 1742
BtuMI	TCGCGA	1 cut(s)	392
Cac8I	GCNNGC	8 cut(s)	40, 166, 226, 230, 365, 1691, 1713, 1962
CaiI	CAGNNNCTG	1 cut(s)	281
CciI	TCATGA	1 cut(s)	1074
CfoI	GCGC	1 cut(s)	1689
Cfr10I	RCCGGY	4 cut(s)	164, 576, 596, 1960
Cfr13I	GGNCC	6 cut(s)	244, 361, 384, 1563, 1617, 1958
ClaI	ATCGAT	1 cut(s)	423
CseI	GACGC	2 cut(s)	730, 1373
Csp6I	GTAC	7 cut(s)	94, 273, 709, 1328, 1666, 1723, 1828
CspCI	CAANNNNNGTGG	2 cut(s)	1202, 1237
CviQI	GTAC	7 cut(s)	94, 273, 709, 1328, 1666, 1723, 1828
DdeI	CTNAG	2 cut(s)	478, 1911
DpnI	GATC	8 cut(s)	83, 101, 115, 395, 426, 1278, 1353, 1431
DpnII	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
DrdI	GACNNNNNNGTC	1 cut(s)	619
DseDI	GACNNNNNNGTC	1 cut(s)	619
Eam1104I	CTCTTC	4 cut(s)	1067, 1542, 1851, 1993
EarI	CTCTTC	4 cut(s)	1067, 1542, 1851, 1993
EciI	GGCGGA	1 cut(s)	1271
Ecl136II	GAGCTC	1 cut(s)	1476
Eco130I	CCWWGG	1 cut(s)	247
Eco24I	GRGCYC	1 cut(s)	1478
Eco47I	GGWCC	1 cut(s)	1617
Eco53kI	GAGCTC	1 cut(s)	1476
Eco57I	CTGAAG	2 cut(s)	1647, 1674
Eco91I	GGTNACC	1 cut(s)	259
EcoICRI	GAGCTC	1 cut(s)	1476
EcoO109I	RGGNCCY	1 cut(s)	1617
EcoO65I	GGTNACC	1 cut(s)	259
EcoRII	CCWGG	4 cut(s)	109, 337, 380, 1400
EcoT14I	CCWWGG	1 cut(s)	247
EcoT22I	ATGCAT	1 cut(s)	161
EcoT38I	GRGCYC	1 cut(s)	1478
ErhI	CCWWGG	1 cut(s)	247
FalI	AAGNNNNNCTT	4 cut(s)	398, 430, 857, 889
FaqI	GGGAC	1 cut(s)	1603
FauI	CCCGC	3 cut(s)	351, 1122, 1521
FauNDI	CATATG	3 cut(s)	22, 155, 1370
FokI	GGATG	3 cut(s)	748, 1171, 1375
FriOI	GRGCYC	1 cut(s)	1478
FspBI	CTAG	5 cut(s)	345, 846, 1055, 1361, 1893
GlaI	GCGC	1 cut(s)	1688
HaeII	RGCGCY	1 cut(s)	1690
HaeIII	GGCC	6 cut(s)	168, 246, 363, 386, 1565, 1960
HapII	CCGG	5 cut(s)	165, 577, 597, 1181, 1961
HgaI	GACGC	2 cut(s)	730, 1373
HhaI	GCGC	1 cut(s)	1689
Hin1I	GRCGYC	1 cut(s)	1365
Hin6I	GCGC	1 cut(s)	1687
HinP1I	GCGC	1 cut(s)	1687
HinfI	GANTC	3 cut(s)	1385, 1877, 2005
HpaII	CCGG	5 cut(s)	165, 577, 597, 1181, 1961
HphI	GGTGA	3 cut(s)	253, 1439, 2021
Hpy166II	GTNNAC	2 cut(s)	1328, 1376
Hpy188I	TCNGA	4 cut(s)	118, 1460, 1927, 2010
Hpy188III	TCNNGA	7 cut(s)	55, 391, 782, 1075, 1181, 1361, 1881
Hpy8I	GTNNAC	2 cut(s)	1328, 1376
Hpy99I	CGWCG	1 cut(s)	1206
HpyAV	CCTTC	6 cut(s)	274, 1418, 1488, 1601, 1773, 2005
HpyCH4III	ACNGT	4 cut(s)	277, 1169, 1612, 2061
HpyCH4IV	ACGT	3 cut(s)	206, 1007, 1668
HpyF3I	CTNAG	2 cut(s)	478, 1911
HpySE526I	ACGT	3 cut(s)	206, 1007, 1668
Hsp92I	GRCGYC	1 cut(s)	1365
HspAI	GCGC	1 cut(s)	1687
Kpn2I	TCCGGA	1 cut(s)	1180
KpnI	GGTACC	1 cut(s)	97
KroI	GCCGGC	2 cut(s)	164, 1960
KroNI	GCCGGC	2 cut(s)	166, 1962
Kzo9I	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
LguI	GCTCTTC	1 cut(s)	1851
LmnI	GCTCC	5 cut(s)	674, 1179, 1183, 1481, 1849
Lsp1109I	GCAGC	7 cut(s)	16, 484, 641, 844, 1173, 1720, 1972
LweI	GCATC	7 cut(s)	168, 261, 844, 1074, 1434, 1560, 2027
MaeI	CTAG	5 cut(s)	345, 846, 1055, 1361, 1893
MaeII	ACGT	3 cut(s)	206, 1007, 1668
MaeIII	GTNAC	2 cut(s)	124, 259
MalI	GATC	8 cut(s)	83, 101, 115, 395, 426, 1278, 1353, 1431
MboI	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
MboII	GAAGA	9 cut(s)	91, 136, 184, 280, 1054, 1559, 1667, 1868, 2010
MflI	RGATCY	4 cut(s)	81, 99, 113, 1276
MhlI	GDGCHC	2 cut(s)	534, 1478
MluI	ACGCGT	1 cut(s)	121
MlyI	GAGTC	1 cut(s)	1394
MmeI	TCCRAC	1 cut(s)	652
Mph1103I	ATGCAT	1 cut(s)	161
MroI	TCCGGA	1 cut(s)	1180
MroNI	GCCGGC	2 cut(s)	164, 1960
MseI	TTAA	3 cut(s)	372, 902, 996
MslI	CAYNNNNRTG	3 cut(s)	1735, 1866, 2034
MspA1I	CMGCKG	2 cut(s)	29, 497
MspI	CCGG	5 cut(s)	165, 577, 597, 1181, 1961
MspR9I	CCNGG	4 cut(s)	111, 339, 382, 1402
Mva1269I	GAATGC	2 cut(s)	719, 1032
MvaI	CCWGG	4 cut(s)	111, 339, 382, 1402
MvnI	CGCG	3 cut(s)	123, 392, 807
NaeI	GCCGGC	2 cut(s)	166, 1962
NdeI	CATATG	3 cut(s)	22, 155, 1370
NdeII	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
NgoMIV	GCCGGC	2 cut(s)	164, 1960
NlaIV	GGNNCC	6 cut(s)	95, 115, 137, 362, 1166, 1619
NmeAIII	GCCGAG	1 cut(s)	14
NmuCI	GTSAC	2 cut(s)	124, 259
NruI	TCGCGA	1 cut(s)	392
NsiI	ATGCAT	1 cut(s)	161
NspI	RCATGY	3 cut(s)	232, 1715, 1844
NspV	TTCGAA	1 cut(s)	319
OliI	CACNNNNGTG	1 cut(s)	1735
PaeI	GCATGC	2 cut(s)	232, 1715
PagI	TCATGA	1 cut(s)	1074
PasI	CCCWGGG	1 cut(s)	381
PciI	ACATGT	1 cut(s)	1840
PciSI	GCTCTTC	1 cut(s)	1851
PcsI	WCGNNNNNNNCGW	1 cut(s)	1928
PctI	GAATGC	2 cut(s)	719, 1032
PdiI	GCCGGC	2 cut(s)	166, 1962
PfeI	GAWTC	2 cut(s)	1877, 2005
PleI	GAGTC	1 cut(s)	1393
PpsI	GAGTC	1 cut(s)	1393
PpuMI	RGGWCCY	1 cut(s)	1617
PscI	ACATGT	1 cut(s)	1840
Psp124BI	GAGCTC	1 cut(s)	1478
Psp5II	RGGWCCY	1 cut(s)	1617
Psp6I	CCWGG	4 cut(s)	109, 337, 380, 1400
PspEI	GGTNACC	1 cut(s)	259
PspGI	CCWGG	4 cut(s)	109, 337, 380, 1400
PspN4I	GGNNCC	6 cut(s)	95, 115, 137, 362, 1166, 1619
PspPI	GGNCC	6 cut(s)	244, 361, 384, 1563, 1617, 1958
PspPPI	RGGWCCY	1 cut(s)	1617
PstI	CTGCAG	1 cut(s)	1710
PstNI	CAGNNNCTG	1 cut(s)	281
PsuI	RGATCY	4 cut(s)	81, 99, 113, 1276
PvuII	CAGCTG	1 cut(s)	497
RruI	TCGCGA	1 cut(s)	392
RsaI	GTAC	7 cut(s)	95, 274, 710, 1329, 1667, 1724, 1829
RsaNI	GTAC	7 cut(s)	94, 273, 709, 1328, 1666, 1723, 1828
RseI	CAYNNNNRTG	3 cut(s)	1735, 1866, 2034
SacI	GAGCTC	1 cut(s)	1478
SapI	GCTCTTC	1 cut(s)	1851
SaqAI	TTAA	3 cut(s)	372, 902, 996
Sau3AI	GATC	8 cut(s)	81, 99, 113, 393, 424, 1276, 1351, 1429
Sau96I	GGNCC	6 cut(s)	244, 361, 384, 1563, 1617, 1958
ScaI	AGTACT	1 cut(s)	1829
SchI	GAGTC	1 cut(s)	1394
ScrFI	CCNGG	4 cut(s)	111, 339, 382, 1402
SduI	GDGCHC	2 cut(s)	534, 1478
SfaNI	GCATC	7 cut(s)	168, 261, 844, 1074, 1434, 1560, 2027
SfcI	CTRYAG	1 cut(s)	1706
SfuI	TTCGAA	1 cut(s)	319
SinI	GGWCC	1 cut(s)	1617
SmiMI	CAYNNNNRTG	3 cut(s)	1735, 1866, 2034
SmlI	CTYRAG	5 cut(s)	404, 1382, 1469, 1523, 1661
SmoI	CTYRAG	5 cut(s)	404, 1382, 1469, 1523, 1661
SpeI	ACTAGT	2 cut(s)	845, 1892
SphI	GCATGC	2 cut(s)	232, 1715
SspI	AATATT	1 cut(s)	941
SspMI	CTAG	5 cut(s)	345, 846, 1055, 1361, 1893
SstI	GAGCTC	1 cut(s)	1478
StyD4I	CCNGG	4 cut(s)	109, 337, 380, 1400
StyI	CCWWGG	1 cut(s)	247
TaaI	ACNGT	4 cut(s)	277, 1169, 1612, 2061
TaiI	ACGT	3 cut(s)	209, 1010, 1671
TaqI	TCGA	8 cut(s)	56, 105, 319, 333, 423, 550, 593, 1601
TatI	WGTACW	1 cut(s)	1827
TauI	GCSGC	6 cut(s)	29, 224, 434, 1287, 1545, 1942
TfiI	GAWTC	2 cut(s)	1877, 2005
Tru1I	TTAA	3 cut(s)	372, 902, 996
Tru9I	TTAA	3 cut(s)	372, 902, 996
TscAI	CASTG	4 cut(s)	486, 1021, 1617, 1742
TseFI	GTSAC	2 cut(s)	124, 259
TseI	GCWGC	7 cut(s)	29, 497, 629, 857, 1186, 1708, 1985
Tsp45I	GTSAC	2 cut(s)	124, 259
TspDTI	ATGAA	5 cut(s)	669, 969, 1441, 1890, 2052
TspGWI	ACGGA	2 cut(s)	260, 999
TspRI	CASTG	4 cut(s)	486, 1021, 1617, 1742
VpaK11BI	GGWCC	1 cut(s)	1617
XapI	RAATTY	4 cut(s)	314, 771, 970, 1979
XbaI	TCTAGA	1 cut(s)	1360
XceI	RCATGY	3 cut(s)	232, 1715, 1844
XspI	CTAG	5 cut(s)	345, 846, 1055, 1361, 1893
ZrmI	AGTACT	1 cut(s)	1829
Zsp2I	ATGCAT	1 cut(s)	161

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.