Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0009501

Physical Location & Seq

Genomic Coordinates Forward (+)

34304742 .. 34307722

Gene Structure 2981 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1210000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2040 bp

ATGGACTGGGCTGCAAACAGCGGTTTCAAAACCTTCAAAGATGTGGAACCCAAATCAATGATGGACATGGCGATGATTCCAATCGTCGATCCGGTGGATATCGGTTTGGGCTCCTCGGAACAGGGGAATTCTACTCCTTCAACCAAACCCAGAAAGAAGACTATGACGTCGGTTTATCTCAAGTTTTTCGAGACAGCTTCTGACGGAAAAAGTCGCCGGTGCAAGTTTTGTGGACAAAGCTATTCCATTGCAACTGCCACTGGTAATCTGGGAAGGCACCTAAGTAATCGCCATCCCGGTTATGATAAGTCGGGCGATGTTGTCACCAGTCCTGCACCACAATCCGCCATTGTAGCCCGGAAACATCAACCTCAATCCCAATCAAAAGCATCCCAATTGGATTACAACCATATAAACTGGTTGCTTGTTAAATGGCTCGTCTTAGCTTCTTTGCCTCCGGCAACATTGGAAGAGAAGTGGGTGGCAAACTCGTACAAGTTTTTAAATCCATCCGTACAACTATGGTCAAGCGAGGAGTACAGAAGTACATTTTACGAAGTTTTCCAGAGTATGCAGGAAGTAGTAAGACTTTCACTCGAACAAGTTTCTTCGAAGGTCTCTATCACACTTGAATTCTGGACTTCCTATGAGGAAATTAGTTACATGAGTGTCACATGCCACTGGATCGACGAGAGTTGGTCTTTCCAGAAGATGATGCTCGATATCTGTCACATACCTTACCCGTGTGGTGGCGCCGAGATCTATCACTCCCTGGTGAAGGTTCTTAGGTCGTACAATATTGAGAACAGAGTCCTATCATGCACCTATGATAACAGTCAGAGTGCAATGCATGCTTACCTGGATGGTCAGAAAGTCGGGCCCTTCTGTTATATCCCCTGTTCTGCTCATATTTTGAACTTGATCATAAACGATGGATTGAGAACTACAAAGCCGGTGATCGTAAAGATTCGAGAGTTTGCAATGGGGTTGAACGCGTCCGCGGAGATGGCAGAGGATTTTGCTGAATTCACAGCAGCTTACCAGGAACTTTGTTGGAAAATGCCGCTTGATACATCAACGCGGTGGAGTGGCAATTATCAGATGCTAGATATTGTGTGCAAGGCTTCGAAGTCCATGGATGCTGTTATCAGGAAGTATGAGACACTAGGCAGTAGGATGCTGCTGAGCTCTGCAGAGAAGAATGCAGTCAGTCATGTGCACCGATATCTCGAACCATTCTACAAAACCACAAACAACATGTGCACAGACAAGCTTCCCACTGTCGGGCTGGTTCTTTTCTTTATGGATCACATTTCCGAGACAATTGCTGCTTGCAGAGACTCTCACCTTTACCCAGAATCGTTAAAGAATGCCGCTAAAGAAATGGCTGAAAAGGTTAGAGGTTACAATAGCCAGGTCTGCAACATAATCATATACATGACAGCGATTCTTGATCCTCGAATCAAAGGAGAGCTATTTCCCGAAAGCCTCAACTCGGATAATTTTCTTGACGAAGCAAGAACTCATTTCATCAGAAACTACTCGACCACCCATTTCCCATCCATAGCTGGTGGATACAGTGCTCAAGAAATGGATGAGGGATGCAATGTCTCGTTTGCAGAGGAAATTGCACGAAAGAAACGAAGGGCAAACATGAGCAGCGCAACGGATGAGCTCACTCAATACCTGTCGGAGCCTCCAGCTCCACTAGCAACAGATGTTCTGGAGTGGTGGAAAGTCAATAGTATGCGCTATCCACGGCTTTCTCTGATGGCTCGGGATTTCTTAGCTGTGCAGGCAGTTGCAGTGGCGCCGGAAGAACTATTTTGTGCCAAAGGTGACGAAATTTACAAGCAACGATTCTGTATGCCGCACGGCAGCACACAAACTCTCCTTTGCATAAGGTCGTGGCTTCAGAGTGGGATGAAAATGAAGTACAAATCAACTGAAATAGATTTTGAGCGGTTGATGGAATTGGCAACTAGTGCTGCTGCTGCTGATAATAGTGCTCATGGCTCTGAGAAGAAACAGAAACATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF4413 Dimer_Tnp_hAT zf-BED

Protein Analysis

680

Amino Acids

76.84

Weight (kDa)

6.67

Isoelectric Point (pI)

50.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
hAT-like_RNase-H	PF14372	421 - 514	6.8e-11	hAT-like transposase, RNase-H fold
Dimer_Tnp_hAT	PF05699	558 - 638	3e-22	hAT family C-terminal dimerisation region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009501)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18560
fragaria_vesca	FvH4_5g12801 FvH4_5g12801 FvH4_5g12801 FvH4_5g12801 FvH4_5g12801
malus_domestica	MD06G1210000.v1.1 MD14G1220900.v1.1
prunus_persica	Prupe.5G216200_v2.0.a1 Prupe.5G216200_v2.0.a1
pyrus_communis	pycom06g18760 pycom06g18770 pycom14g18280
rosa_chinensis	RchiOBHm_Chr7g0184431
rosa_multiflora	Rmu_sc0038166.1_g000001 Rmu_sc0039138.1_g000001
rosa_roxburghii	Rroxscaffold_3G00269620
rosa_samantha	Rh7AG080000 Rh7BG067200 Rh7CG067500 Rh7DG067200
rosa_wichuraiana	Rw7G005530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	166
AatII	GACGTC	1 cut(s)	170
AccB1I	GGYRCC	3 cut(s)	276, 752, 1810
AccBSI	CCGCTC	1 cut(s)	1963
AccII	CGCG	3 cut(s)	995, 1001, 1081
AciI	CCGC	9 cut(s)	21, 345, 999, 1001, 1064, 1081, 1374, 1871, 1963
AclWI	GGATC	4 cut(s)	83, 692, 1314, 1448
AcsI	RAATTY	4 cut(s)	127, 632, 1025, 1845
AcuI	CTGAAG	1 cut(s)	1898
AcyI	GRCGYC	3 cut(s)	167, 753, 1811
AfaI	GTAC	6 cut(s)	494, 516, 539, 547, 794, 1937
AfiI	CCNNNNNNNGG	5 cut(s)	749, 778, 1283, 1284, 1495
AflIII	ACRYGT	2 cut(s)	993, 1257
AgsI	TTSAA	6 cut(s)	28, 37, 141, 632, 916, 991
AhlI	ACTAGT	1 cut(s)	1982
AjnI	CCWGG	4 cut(s)	771, 858, 1041, 1413
Alw21I	GWGCWC	6 cut(s)	1190, 1221, 1265, 1585, 1677, 2011
Alw26I	GTCTC	6 cut(s)	185, 622, 1154, 1313, 1332, 1615
Alw44I	GTGCAC	2 cut(s)	1217, 1261
AlwI	GGATC	4 cut(s)	83, 692, 1314, 1448
AlwNI	CAGNNNCTG	1 cut(s)	200
Ama87I	CYCGRG	1 cut(s)	1776
AoxI	GGCC	1 cut(s)	878
ApaI	GGGCCC	1 cut(s)	882
ApaLI	GTGCAC	2 cut(s)	1217, 1261
ApeKI	GCWGC	9 cut(s)	11, 1034, 1180, 1328, 1659, 1878, 1988, 1991, 1994
ApoI	RAATTY	4 cut(s)	127, 632, 1025, 1845
AspLEI	GCGC	4 cut(s)	755, 1664, 1752, 1813
AspS9I	GGNCC	2 cut(s)	878, 879
AsuC2I	CCSGG	2 cut(s)	297, 358
AsuHPI	GGTGA	5 cut(s)	316, 787, 967, 1337, 1850
AsuII	TTCGAA	2 cut(s)	611, 1127
AvaI	CYCGRG	1 cut(s)	1776
BaeGI	GKGCMC	3 cut(s)	882, 1221, 1265
BanI	GGYRCC	3 cut(s)	276, 752, 1810
BanII	GRGCYC	4 cut(s)	113, 882, 1190, 1677
BbsI	GAAGAC	1 cut(s)	164
Bbv12I	GWGCWC	6 cut(s)	1190, 1221, 1265, 1585, 1677, 2011
BbvI	GCAGC	8 cut(s)	1046, 1167, 1315, 1671, 1890, 1975, 1978, 1981
BccI	CCATC	9 cut(s)	55, 300, 517, 857, 926, 1000, 1567, 1765, 1963
BceAI	ACGGC	2 cut(s)	1775, 1891
BcgI	CGANNNNNNTGC	2 cut(s)	1307, 1341
BciT130I	CCWGG	4 cut(s)	773, 860, 1043, 1415
BciVI	GTATCC	1 cut(s)	1568
BclI	TGATCA	1 cut(s)	921
BcnI	CCSGG	2 cut(s)	297, 358
BcoDI	GTCTC	6 cut(s)	185, 622, 1154, 1313, 1332, 1615
BcuI	ACTAGT	1 cut(s)	1982
BfaI	CTAG	4 cut(s)	1106, 1166, 1709, 1983
BfmI	CTRYAG	1 cut(s)	1191
BfoI	RGCGCY	2 cut(s)	756, 1814
BfuI	GTATCC	1 cut(s)	1568
BglII	AGATCT	1 cut(s)	759
BlpI	GCTNAGC	1 cut(s)	1184
Bme1390I	CCNGG	6 cut(s)	297, 358, 773, 860, 1043, 1415
BmeT110I	CYCGRG	1 cut(s)	1776
BmgT120I	GGNCC	2 cut(s)	878, 879
BmiI	GGNNCC	7 cut(s)	48, 112, 278, 754, 880, 1695, 1812
BmrFI	CCNGG	6 cut(s)	297, 358, 773, 860, 1043, 1415
BmrI	ACTGGG	1 cut(s)	16
BmsI	GCATC	6 cut(s)	398, 705, 1092, 1129, 1167, 1592
BmuI	ACTGGG	1 cut(s)	16
BpiI	GAAGAC	1 cut(s)	164
BpmI	CTGGAG	2 cut(s)	1683, 1745
Bpu1102I	GCTNAGC	1 cut(s)	1184
Bpu14I	TTCGAA	2 cut(s)	611, 1127
BpuEI	CTTGAG	2 cut(s)	164, 1569
BpuMI	CCSGG	2 cut(s)	297, 358
BsaBI	GATNNNNATC	1 cut(s)	80
BsaHI	GRCGYC	3 cut(s)	167, 753, 1811
BsaI	GGTCTC	1 cut(s)	622
BsaJI	CCNNGG	5 cut(s)	114, 771, 999, 1134, 1757
BsaWI	WCCGGW	1 cut(s)	91
BsaXI	ACNNNNNCTCC	2 cut(s)	1875, 1905
Bsc4I	CCNNNNNNNGG	5 cut(s)	749, 778, 1283, 1284, 1495
Bse118I	RCCGGY	2 cut(s)	216, 952
Bse1I	ACTGG	5 cut(s)	11, 265, 327, 422, 686
Bse3DI	GCAATG	4 cut(s)	246, 852, 987, 1612
Bse8I	GATNNNNATC	1 cut(s)	80
BseBI	CCWGG	4 cut(s)	773, 860, 1043, 1415
BseDI	CCNNGG	5 cut(s)	114, 771, 999, 1134, 1757
BseJI	GATNNNNATC	1 cut(s)	80
BseLI	CCNNNNNNNGG	5 cut(s)	749, 778, 1283, 1284, 1495
BseMI	GCAATG	4 cut(s)	246, 852, 987, 1612
BseMII	CTCAG	2 cut(s)	1175, 2010
BseNI	ACTGG	5 cut(s)	11, 265, 327, 422, 686
BseRI	GAGGAG	2 cut(s)	103, 548
BseSI	GKGCMC	3 cut(s)	882, 1221, 1265
BseXI	GCAGC	8 cut(s)	1046, 1167, 1315, 1671, 1890, 1975, 1978, 1981
BsgI	GTGCAG	2 cut(s)	318, 1814
Bsh1236I	CGCG	3 cut(s)	995, 1001, 1081
BshFI	GGCC	1 cut(s)	880
BshNI	GGYRCC	3 cut(s)	276, 752, 1810
BsiHKAI	GWGCWC	6 cut(s)	1190, 1221, 1265, 1585, 1677, 2011
BsiHKCI	CYCGRG	1 cut(s)	1776
BsiSI	CCGG	7 cut(s)	92, 217, 297, 358, 458, 953, 1814
BslI	CCNNNNNNNGG	5 cut(s)	749, 778, 1283, 1284, 1495
BsmAI	GTCTC	6 cut(s)	185, 622, 1154, 1313, 1332, 1615
BsmI	GAATGC	2 cut(s)	1207, 1375
BsnI	GGCC	1 cut(s)	880
Bso31I	GGTCTC	1 cut(s)	622
BsoBI	CYCGRG	1 cut(s)	1776
Bsp119I	TTCGAA	2 cut(s)	611, 1127
Bsp120I	GGGCCC	1 cut(s)	878
Bsp1286I	GDGCHC	8 cut(s)	113, 882, 1190, 1221, 1265, 1585, 1677, 2011
Bsp143I	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
Bsp1720I	GCTNAGC	1 cut(s)	1184
Bsp19I	CCATGG	1 cut(s)	1134
BspACI	CCGC	9 cut(s)	21, 345, 999, 1001, 1064, 1081, 1374, 1871, 1963
BspANI	GGCC	1 cut(s)	880
BspCNI	CTCAG	2 cut(s)	1176, 2011
BspFNI	CGCG	3 cut(s)	995, 1001, 1081
BspLI	GGNNCC	7 cut(s)	48, 112, 278, 754, 880, 1695, 1812
BspMAI	CTGCAG	1 cut(s)	1195
BspPI	GGATC	4 cut(s)	83, 692, 1314, 1448
BspT104I	TTCGAA	2 cut(s)	611, 1127
BspT107I	GGYRCC	3 cut(s)	276, 752, 1810
BspTNI	GGTCTC	1 cut(s)	622
BsrBI	CCGCTC	1 cut(s)	1963
BsrDI	GCAATG	4 cut(s)	246, 852, 987, 1612
BsrFI	RCCGGY	2 cut(s)	216, 952
BsrI	ACTGG	5 cut(s)	11, 265, 327, 422, 686
BssAI	RCCGGY	2 cut(s)	216, 952
BssECI	CCNNGG	5 cut(s)	114, 771, 999, 1134, 1757
BssMI	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
BssNI	GRCGYC	3 cut(s)	167, 753, 1811
BssT1I	CCWWGG	1 cut(s)	1134
Bst2UI	CCWGG	4 cut(s)	773, 860, 1043, 1415
Bst4CI	ACNGT	3 cut(s)	836, 1282, 1580
Bst6I	CTCTTC	1 cut(s)	465
BstACI	GRCGYC	3 cut(s)	167, 753, 1811
BstAPI	GCANNNNNTGC	2 cut(s)	851, 1985
BstBI	TTCGAA	2 cut(s)	611, 1127
BstC8I	GCNNGC	3 cut(s)	852, 1333, 1797
BstDEI	CTNAG	6 cut(s)	281, 442, 785, 1184, 1786, 2019
BstDSI	CCRYGG	3 cut(s)	999, 1134, 1757
BstENI	CCTNNNNNAGG	1 cut(s)	776
BstFNI	CGCG	3 cut(s)	995, 1001, 1081
BstH2I	RGCGCY	2 cut(s)	756, 1814
BstHHI	GCGC	4 cut(s)	755, 1664, 1752, 1813
BstKTI	GATC	7 cut(s)	91, 687, 762, 924, 960, 1309, 1456
BstMAI	GTCTC	6 cut(s)	185, 622, 1154, 1313, 1332, 1615
BstMBI	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
BstMWI	GCNNNNNNNGC	8 cut(s)	353, 851, 1007, 1419, 1709, 1796, 1985, 1994
BstNI	CCWGG	4 cut(s)	773, 860, 1043, 1415
BstNSI	RCATGY	3 cut(s)	678, 854, 1261
BstSCI	CCNGG	6 cut(s)	295, 356, 771, 858, 1041, 1413
BstSFI	CTRYAG	1 cut(s)	1191
BstSLI	GKGCMC	3 cut(s)	882, 1221, 1265
BstUI	CGCG	3 cut(s)	995, 1001, 1081
BstV1I	GCAGC	8 cut(s)	1046, 1167, 1315, 1671, 1890, 1975, 1978, 1981
BstV2I	GAAGAC	1 cut(s)	164
BstX2I	RGATCY	1 cut(s)	759
BstYI	RGATCY	1 cut(s)	759
BsuI	GTATCC	1 cut(s)	1568
BsuRI	GGCC	1 cut(s)	880
BtgI	CCRYGG	3 cut(s)	999, 1134, 1757
BtgZI	GCGATG	2 cut(s)	86, 330
BtsI	GCAGTG	1 cut(s)	1812
BtsIMutI	CAGTG	5 cut(s)	258, 679, 1278, 1585, 1812
Cac8I	GCNNGC	3 cut(s)	852, 1333, 1797
CaiI	CAGNNNCTG	1 cut(s)	200
CfoI	GCGC	4 cut(s)	755, 1664, 1752, 1813
Cfr10I	RCCGGY	2 cut(s)	216, 952
Cfr13I	GGNCC	2 cut(s)	878, 879
Cfr42I	CCGCGG	1 cut(s)	1002
CseI	GACGC	1 cut(s)	984
Csp6I	GTAC	6 cut(s)	493, 515, 538, 546, 793, 1936
CspCI	CAANNNNNGTGG	2 cut(s)	211, 246
CviQI	GTAC	6 cut(s)	493, 515, 538, 546, 793, 1936
DdeI	CTNAG	6 cut(s)	281, 442, 785, 1184, 1786, 2019
DinI	GGCGCC	2 cut(s)	754, 1812
DpnI	GATC	7 cut(s)	90, 686, 761, 923, 959, 1308, 1455
DpnII	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
DraI	TTTAAA	1 cut(s)	504
DrdI	GACNNNNNNGTC	1 cut(s)	166
DseDI	GACNNNNNNGTC	1 cut(s)	166
Eam1104I	CTCTTC	1 cut(s)	465
EarI	CTCTTC	1 cut(s)	465
EciI	GGCGGA	1 cut(s)	334
Ecl136II	GAGCTC	2 cut(s)	1188, 1675
Eco130I	CCWWGG	1 cut(s)	1134
Eco24I	GRGCYC	4 cut(s)	113, 882, 1190, 1677
Eco31I	GGTCTC	1 cut(s)	622
Eco32I	GATATC	3 cut(s)	100, 724, 1226
Eco53kI	GAGCTC	2 cut(s)	1188, 1675
Eco57I	CTGAAG	1 cut(s)	1898
Eco88I	CYCGRG	1 cut(s)	1776
EcoICRI	GAGCTC	2 cut(s)	1188, 1675
EcoNI	CCTNNNNNAGG	1 cut(s)	776
EcoO109I	RGGNCCY	1 cut(s)	879
EcoRI	GAATTC	3 cut(s)	127, 632, 1025
EcoRII	CCWGG	4 cut(s)	771, 858, 1041, 1413
EcoRV	GATATC	3 cut(s)	100, 724, 1226
EcoT14I	CCWWGG	1 cut(s)	1134
EcoT22I	ATGCAT	1 cut(s)	852
EcoT38I	GRGCYC	4 cut(s)	113, 882, 1190, 1677
EgeI	GGCGCC	2 cut(s)	754, 1812
EheI	GGCGCC	2 cut(s)	754, 1812
ErhI	CCWWGG	1 cut(s)	1134
FbaI	TGATCA	1 cut(s)	921
FriOI	GRGCYC	4 cut(s)	113, 882, 1190, 1677
FspBI	CTAG	4 cut(s)	1106, 1166, 1709, 1983
GlaI	GCGC	4 cut(s)	754, 1663, 1751, 1812
GsuI	CTGGAG	2 cut(s)	1683, 1745
HaeII	RGCGCY	2 cut(s)	756, 1814
HaeIII	GGCC	1 cut(s)	880
HapII	CCGG	7 cut(s)	92, 217, 297, 358, 458, 953, 1814
HgaI	GACGC	1 cut(s)	984
HhaI	GCGC	4 cut(s)	755, 1664, 1752, 1813
Hin1I	GRCGYC	3 cut(s)	167, 753, 1811
Hin6I	GCGC	4 cut(s)	753, 1662, 1750, 1811
HinP1I	GCGC	4 cut(s)	753, 1662, 1750, 1811
HindIII	AAGCTT	1 cut(s)	1271
HinfI	GANTC	8 cut(s)	76, 810, 967, 1340, 1358, 1447, 1461, 1860
HpaII	CCGG	7 cut(s)	92, 217, 297, 358, 458, 953, 1814
HphI	GGTGA	5 cut(s)	316, 787, 967, 1337, 1850
Hpy166II	GTNNAC	3 cut(s)	233, 1219, 1263
Hpy8I	GTNNAC	3 cut(s)	233, 1219, 1263
Hpy99I	CGWCG	3 cut(s)	89, 172, 692
HpyAV	CCTTC	7 cut(s)	43, 147, 267, 607, 772, 892, 1638
HpyCH4III	ACNGT	3 cut(s)	836, 1282, 1580
HpyCH4IV	ACGT	1 cut(s)	167
HpyF10VI	GCNNNNNNNGC	8 cut(s)	353, 851, 1007, 1419, 1709, 1796, 1985, 1994
HpyF3I	CTNAG	6 cut(s)	281, 442, 785, 1184, 1786, 2019
HpySE526I	ACGT	1 cut(s)	167
Hsp92I	GRCGYC	3 cut(s)	167, 753, 1811
HspAI	GCGC	4 cut(s)	753, 1662, 1750, 1811
KasI	GGCGCC	2 cut(s)	752, 1810
Ksp22I	TGATCA	1 cut(s)	921
KspI	CCGCGG	1 cut(s)	1002
Kzo9I	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
LmnI	GCTCC	3 cut(s)	116, 1693, 1708
Lsp1109I	GCAGC	8 cut(s)	1046, 1167, 1315, 1671, 1890, 1975, 1978, 1981
LweI	GCATC	6 cut(s)	398, 705, 1092, 1129, 1167, 1592
MaeI	CTAG	4 cut(s)	1106, 1166, 1709, 1983
MaeII	ACGT	1 cut(s)	167
MaeIII	GTNAC	6 cut(s)	322, 659, 670, 728, 1403, 1838
MalI	GATC	7 cut(s)	90, 686, 761, 923, 959, 1308, 1455
MbiI	CCGCTC	1 cut(s)	1963
MboI	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
MboII	GAAGA	7 cut(s)	169, 482, 600, 721, 1210, 1829, 2035
MfeI	CAATTG	2 cut(s)	395, 1323
MflI	RGATCY	1 cut(s)	759
MhlI	GDGCHC	8 cut(s)	113, 882, 1190, 1221, 1265, 1585, 1677, 2011
MluI	ACGCGT	1 cut(s)	993
Mly113I	GGCGCC	2 cut(s)	753, 1811
MlyI	GAGTC	2 cut(s)	819, 1334
MmeI	TCCRAC	2 cut(s)	1034, 1671
Mph1103I	ATGCAT	1 cut(s)	852
MseI	TTAA	3 cut(s)	429, 503, 1364
MslI	CAYNNNNRTG	2 cut(s)	71, 1436
MspA1I	CMGCKG	2 cut(s)	21, 1001
MspI	CCGG	7 cut(s)	92, 217, 297, 358, 458, 953, 1814
MspR9I	CCNGG	6 cut(s)	297, 358, 773, 860, 1043, 1415
MunI	CAATTG	2 cut(s)	395, 1323
Mva1269I	GAATGC	2 cut(s)	1207, 1375
MvaI	CCWGG	4 cut(s)	773, 860, 1043, 1415
MvnI	CGCG	3 cut(s)	995, 1001, 1081
MwoI	GCNNNNNNNGC	8 cut(s)	353, 851, 1007, 1419, 1709, 1796, 1985, 1994
NarI	GGCGCC	2 cut(s)	753, 1811
NciI	CCSGG	2 cut(s)	297, 358
NcoI	CCATGG	1 cut(s)	1134
NdeII	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
NlaIV	GGNNCC	7 cut(s)	48, 112, 278, 754, 880, 1695, 1812
NmeAIII	GCCGAG	1 cut(s)	781
NmuCI	GTSAC	4 cut(s)	322, 670, 728, 1838
NsiI	ATGCAT	1 cut(s)	852
NspI	RCATGY	3 cut(s)	678, 854, 1261
NspV	TTCGAA	2 cut(s)	611, 1127
PaeI	GCATGC	1 cut(s)	854
PciI	ACATGT	1 cut(s)	1257
PcsI	WCGNNNNNNNCGW	1 cut(s)	1639
PctI	GAATGC	2 cut(s)	1207, 1375
PfeI	GAWTC	6 cut(s)	76, 967, 1358, 1447, 1461, 1860
PleI	GAGTC	2 cut(s)	818, 1334
PluTI	GGCGCC	2 cut(s)	756, 1814
PpsI	GAGTC	2 cut(s)	818, 1334
PscI	ACATGT	1 cut(s)	1257
Psp124BI	GAGCTC	2 cut(s)	1190, 1677
Psp6I	CCWGG	4 cut(s)	771, 858, 1041, 1413
PspGI	CCWGG	4 cut(s)	771, 858, 1041, 1413
PspN4I	GGNNCC	7 cut(s)	48, 112, 278, 754, 880, 1695, 1812
PspOMI	GGGCCC	1 cut(s)	878
PspPI	GGNCC	2 cut(s)	878, 879
PstI	CTGCAG	1 cut(s)	1195
PstNI	CAGNNNCTG	1 cut(s)	200
PsuI	RGATCY	1 cut(s)	759
RsaI	GTAC	6 cut(s)	494, 516, 539, 547, 794, 1937
RsaNI	GTAC	6 cut(s)	493, 515, 538, 546, 793, 1936
RseI	CAYNNNNRTG	2 cut(s)	71, 1436
SacI	GAGCTC	2 cut(s)	1190, 1677
SacII	CCGCGG	1 cut(s)	1002
SaqAI	TTAA	3 cut(s)	429, 503, 1364
Sau3AI	GATC	7 cut(s)	88, 684, 759, 921, 957, 1306, 1453
Sau96I	GGNCC	2 cut(s)	878, 879
SchI	GAGTC	2 cut(s)	819, 1334
ScrFI	CCNGG	6 cut(s)	297, 358, 773, 860, 1043, 1415
SduI	GDGCHC	8 cut(s)	113, 882, 1190, 1221, 1265, 1585, 1677, 2011
SfaNI	GCATC	6 cut(s)	398, 705, 1092, 1129, 1167, 1592
SfcI	CTRYAG	1 cut(s)	1191
SfoI	GGCGCC	2 cut(s)	754, 1812
Sfr303I	CCGCGG	1 cut(s)	1002
SfuI	TTCGAA	2 cut(s)	611, 1127
SgrAI	CRCCGGYG	1 cut(s)	216
SgrBI	CCGCGG	1 cut(s)	1002
SmiMI	CAYNNNNRTG	2 cut(s)	71, 1436
SmlI	CTYRAG	2 cut(s)	179, 1584
SmoI	CTYRAG	2 cut(s)	179, 1584
SpeI	ACTAGT	1 cut(s)	1982
SphI	GCATGC	1 cut(s)	854
SsiI	CCGC	9 cut(s)	21, 345, 999, 1001, 1064, 1081, 1374, 1871, 1963
SspDI	GGCGCC	2 cut(s)	752, 1810
SspI	AATATT	1 cut(s)	799
SspMI	CTAG	4 cut(s)	1106, 1166, 1709, 1983
SstI	GAGCTC	2 cut(s)	1190, 1677
StyD4I	CCNGG	6 cut(s)	295, 356, 771, 858, 1041, 1413
StyI	CCWWGG	1 cut(s)	1134
TaaI	ACNGT	3 cut(s)	836, 1282, 1580
TaiI	ACGT	1 cut(s)	170
TatI	WGTACW	3 cut(s)	537, 545, 1935
TauI	GCSGC	3 cut(s)	1066, 1376, 1873
TfiI	GAWTC	6 cut(s)	76, 967, 1358, 1447, 1461, 1860
Tru1I	TTAA	3 cut(s)	429, 503, 1364
Tru9I	TTAA	3 cut(s)	429, 503, 1364
TscAI	CASTG	5 cut(s)	265, 686, 1285, 1585, 1812
TseFI	GTSAC	4 cut(s)	322, 670, 728, 1838
TseI	GCWGC	9 cut(s)	11, 1034, 1180, 1328, 1659, 1878, 1988, 1991, 1994
Tsp45I	GTSAC	4 cut(s)	322, 670, 728, 1838
TspDTI	ATGAA	3 cut(s)	1519, 1940, 1946
TspGWI	ACGGA	3 cut(s)	219, 502, 1682
TspRI	CASTG	5 cut(s)	265, 686, 1285, 1585, 1812
VneI	GTGCAC	2 cut(s)	1217, 1261
XagI	CCTNNNNNAGG	1 cut(s)	776
XapI	RAATTY	4 cut(s)	127, 632, 1025, 1845
XceI	RCATGY	3 cut(s)	678, 854, 1261
XcmI	CCANNNNNNNNNTGG	3 cut(s)	58, 265, 1285
XspI	CTAG	4 cut(s)	1106, 1166, 1709, 1983
ZraI	GACGTC	1 cut(s)	168
Zsp2I	ATGCAT	1 cut(s)	852

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.