pycom06g18760

BED zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0009501
Physical Location & Seq
Forward (+)
22371896 .. 22373364
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18760.1

Sequence Viewer

Length: 1017 bp
ATGGATTGGGCTGCAAACACCGGTTTCAAAACCTTCAAAGATGTGGAACCCAAATCAATGATGGACATGGCGATGATTCCAATCGTTGATCCGGTGGATATCGGTTTGGGCTCCTCGGAACAGGCGAATTCTACTCCTTCAACCAAACCCAGAAAGAAGACTATGACGTCGGTTTATCTCAAGTTTTTCGAGACAGCTTCTGACGGAAAAAGTCGCCGGTGCAAGTTTTGTGGACAAAGCTATTCCATTGCAACTGCCACTGGTAATCTGGGAAGGCACCTAAGTAATCGCCATCCCGGTTATGATAAGTCTGGCGATGTTGTCACCAGTCCTGCACCACAATCCGCCATTGTAGCCCGGAAACATCAACCTCAATCTCAATCAAAAGCATCCCAATTGGATTACAACCATATAAACTGGTTGCTTGTTAAATGGCTCGTCTTAGCTTCTTTGCCTCCGGCAACATTGGAAGAGAAGTGGGTGGCAAACTCGTACAAGTTTTTAAATCCATCCGTACAACTATGGTCAAGCGAGGAGTACAAAAGTACATTTTACGAAGTTTTCCAGAGTATGCAGGAAGTAGTAAGACTTTCACTCGAACATGTTTCTTCGAAGGTCTCTATCACACTTGAATTCTGGACTTCCTATGAGGAAATTAGTTACATGAGTGTCACATGCCATTGGATCGACGAGAGTTGGTCTTTCCAGAAGATGATACTCGATATCTGTCACATACCTTACCCGTGTGGTGGCGCCGAGATTTATCACTCTCTGGTGAAGGTTCTTAGGTCGTACAATATTGAGAACAGAGTCCTGTCATGCACCTATGATAACAGTCAGAGTGCAATGCATGCCTACCTGGATGGTCAGAAAGTTGGGCCCTTCTGTTATATCCCCTGTTCTGCTCATATTTTGAACTTGATCGATAAACGATGGATTGAGAACTACAAAGCCGGTGATCGCAAAGATCCGAGAGTTTGCAATGGGGCTAAACGCGTCCAAGGAGATGGCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4413 Dimer_Tnp_hAT zf-BED

Protein Analysis

339

Amino Acids

38.32

Weight (kDa)

8.45

Isoelectric Point (pI)

57.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-BED PF02892 58 - 99 9.3e-06 BED zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 166
AatII GACGTC 1 cut(s) 170
AccB1I GGYRCC 2 cut(s) 276, 752
AccII CGCG 1 cut(s) 996
AciI CCGC 1 cut(s) 345
AclWI GGATC 3 cut(s) 83, 692, 962
AcsI RAATTY 2 cut(s) 127, 632
AcyI GRCGYC 2 cut(s) 167, 753
AfaI GTAC 5 cut(s) 494, 516, 539, 547, 794
AfiI CCNNNNNNNGG 1 cut(s) 749
AflIII ACRYGT 2 cut(s) 601, 994
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 5 cut(s) 28, 37, 141, 632, 916
AjnI CCWGG 1 cut(s) 858
AluBI AGCT 3 cut(s) 197, 240, 446
AluI AGCT 3 cut(s) 197, 240, 446
Alw26I GTCTC 2 cut(s) 185, 622
AlwI GGATC 3 cut(s) 83, 692, 962
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 1 cut(s) 878
ApaI GGGCCC 1 cut(s) 882
ApeKI GCWGC 1 cut(s) 11
ApoI RAATTY 2 cut(s) 127, 632
AsiGI ACCGGT 1 cut(s) 20
AspLEI GCGC 1 cut(s) 755
AspS9I GGNCC 2 cut(s) 878, 879
AsuC2I CCSGG 2 cut(s) 297, 358
AsuHPI GGTGA 3 cut(s) 316, 787, 968
AsuII TTCGAA 1 cut(s) 611
BaeGI GKGCMC 1 cut(s) 882
BanI GGYRCC 2 cut(s) 276, 752
BanII GRGCYC 2 cut(s) 113, 882
BbsI GAAGAC 1 cut(s) 164
BccI CCATC 6 cut(s) 55, 300, 517, 857, 927, 1001
BciT130I CCWGG 1 cut(s) 860
BcnI CCSGG 2 cut(s) 297, 358
BcoDI GTCTC 2 cut(s) 185, 622
BfoI RGCGCY 1 cut(s) 756
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 3 cut(s) 297, 358, 860
BmgT120I GGNCC 2 cut(s) 878, 879
BmiI GGNNCC 5 cut(s) 48, 112, 278, 754, 880
BmrFI CCNGG 3 cut(s) 297, 358, 860
BmsI GCATC 1 cut(s) 398
BpiI GAAGAC 1 cut(s) 164
Bpu14I TTCGAA 1 cut(s) 611
BpuEI CTTGAG 1 cut(s) 164
BpuMI CCSGG 2 cut(s) 297, 358
Bsa29I ATCGAT 1 cut(s) 924
BsaBI GATNNNNATC 1 cut(s) 80
BsaHI GRCGYC 2 cut(s) 167, 753
BsaI GGTCTC 1 cut(s) 622
BsaJI CCNNGG 2 cut(s) 114, 1000
BsaWI WCCGGW 2 cut(s) 20, 91
Bsc4I CCNNNNNNNGG 1 cut(s) 749
Bse118I RCCGGY 3 cut(s) 20, 216, 953
Bse1I ACTGG 3 cut(s) 265, 327, 422
Bse3DI GCAATG 3 cut(s) 246, 852, 988
Bse8I GATNNNNATC 1 cut(s) 80
BseBI CCWGG 1 cut(s) 860
BseCI ATCGAT 1 cut(s) 924
BseDI CCNNGG 2 cut(s) 114, 1000
BseGI GGATG 4 cut(s) 292, 389, 509, 868
BseJI GATNNNNATC 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 749
BseMI GCAATG 3 cut(s) 246, 852, 988
BseNI ACTGG 3 cut(s) 265, 327, 422
BseRI GAGGAG 2 cut(s) 103, 548
BseSI GKGCMC 1 cut(s) 882
BsgI GTGCAG 1 cut(s) 318
Bsh1236I CGCG 1 cut(s) 996
BshFI GGCC 1 cut(s) 880
BshNI GGYRCC 2 cut(s) 276, 752
BshTI ACCGGT 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 924
BsiSI CCGG 7 cut(s) 21, 92, 217, 297, 358, 458, 954
BslI CCNNNNNNNGG 1 cut(s) 749
BsmAI GTCTC 2 cut(s) 185, 622
BsnI GGCC 1 cut(s) 880
Bso31I GGTCTC 1 cut(s) 622
Bsp119I TTCGAA 1 cut(s) 611
Bsp120I GGGCCC 1 cut(s) 878
Bsp1286I GDGCHC 2 cut(s) 113, 882
Bsp143I GATC 5 cut(s) 88, 684, 921, 958, 967
BspACI CCGC 1 cut(s) 345
BspANI GGCC 1 cut(s) 880
BspDI ATCGAT 1 cut(s) 924
BspFNI CGCG 1 cut(s) 996
BspLI GGNNCC 5 cut(s) 48, 112, 278, 754, 880
BspPI GGATC 3 cut(s) 83, 692, 962
BspT104I TTCGAA 1 cut(s) 611
BspT107I GGYRCC 2 cut(s) 276, 752
BspTNI GGTCTC 1 cut(s) 622
BsrDI GCAATG 3 cut(s) 246, 852, 988
BsrFI RCCGGY 3 cut(s) 20, 216, 953
BsrI ACTGG 3 cut(s) 265, 327, 422
BssAI RCCGGY 3 cut(s) 20, 216, 953
BssECI CCNNGG 2 cut(s) 114, 1000
BssMI GATC 5 cut(s) 88, 684, 921, 958, 967
BssNI GRCGYC 2 cut(s) 167, 753
BssT1I CCWWGG 1 cut(s) 1000
Bst2UI CCWGG 1 cut(s) 860
Bst4CI ACNGT 1 cut(s) 836
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 2 cut(s) 167, 753
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 611
BstC8I GCNNGC 1 cut(s) 852
BstDEI CTNAG 3 cut(s) 281, 442, 785
BstF5I GGATG 4 cut(s) 292, 389, 509, 868
BstFNI CGCG 1 cut(s) 996
BstH2I RGCGCY 1 cut(s) 756
BstHHI GCGC 1 cut(s) 755
BstKTI GATC 5 cut(s) 91, 687, 924, 961, 970
BstMAI GTCTC 2 cut(s) 185, 622
BstMBI GATC 5 cut(s) 88, 684, 921, 958, 967
BstMWI GCNNNNNNNGC 2 cut(s) 353, 851
BstNI CCWGG 1 cut(s) 860
BstNSI RCATGY 3 cut(s) 605, 678, 854
BstSCI CCNGG 3 cut(s) 295, 356, 858
BstSLI GKGCMC 1 cut(s) 882
BstUI CGCG 1 cut(s) 996
BstV2I GAAGAC 1 cut(s) 164
BstX2I RGATCY 1 cut(s) 967
BstXI CCANNNNNNTGG 1 cut(s) 1007
BstYI RGATCY 1 cut(s) 967
Bsu15I ATCGAT 1 cut(s) 924
BsuRI GGCC 1 cut(s) 880
BsuTUI ATCGAT 1 cut(s) 924
BtgZI GCGATG 2 cut(s) 86, 330
BtsCI GGATG 4 cut(s) 292, 389, 509, 868
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 1 cut(s) 852
CaiI CAGNNNCTG 1 cut(s) 200
CfoI GCGC 1 cut(s) 755
Cfr10I RCCGGY 3 cut(s) 20, 216, 953
Cfr13I GGNCC 2 cut(s) 878, 879
ClaI ATCGAT 1 cut(s) 924
CseI GACGC 1 cut(s) 985
Csp6I GTAC 5 cut(s) 493, 515, 538, 546, 793
CspAI ACCGGT 1 cut(s) 20
CspCI CAANNNNNGTGG 2 cut(s) 211, 246
CviAII CATG 6 cut(s) 67, 602, 664, 675, 819, 851
CviQI GTAC 5 cut(s) 493, 515, 538, 546, 793
DdeI CTNAG 3 cut(s) 281, 442, 785
DinI GGCGCC 1 cut(s) 754
DpnI GATC 5 cut(s) 90, 686, 923, 960, 969
DpnII GATC 5 cut(s) 88, 684, 921, 958, 967
DraI TTTAAA 1 cut(s) 504
DrdI GACNNNNNNGTC 1 cut(s) 166
DseDI GACNNNNNNGTC 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 334
Eco130I CCWWGG 1 cut(s) 1000
Eco24I GRGCYC 2 cut(s) 113, 882
Eco31I GGTCTC 1 cut(s) 622
Eco32I GATATC 2 cut(s) 100, 724
EcoO109I RGGNCCY 1 cut(s) 879
EcoRI GAATTC 2 cut(s) 127, 632
EcoRII CCWGG 1 cut(s) 858
EcoRV GATATC 2 cut(s) 100, 724
EcoT14I CCWWGG 1 cut(s) 1000
EcoT22I ATGCAT 1 cut(s) 852
EcoT38I GRGCYC 2 cut(s) 113, 882
EgeI GGCGCC 1 cut(s) 754
EheI GGCGCC 1 cut(s) 754
ErhI CCWWGG 1 cut(s) 1000
FaeI CATG 6 cut(s) 70, 605, 667, 678, 822, 854
FatI CATG 6 cut(s) 66, 601, 663, 674, 818, 850
Fnu4HI GCNGC 1 cut(s) 12
FokI GGATG 4 cut(s) 279, 376, 496, 875
FriOI GRGCYC 2 cut(s) 113, 882
Fsp4HI GCNGC 1 cut(s) 12
GlaI GCGC 1 cut(s) 754
GluI GCNGC 1 cut(s) 12
HaeII RGCGCY 1 cut(s) 756
HaeIII GGCC 1 cut(s) 880
HapII CCGG 7 cut(s) 21, 92, 217, 297, 358, 458, 954
HgaI GACGC 1 cut(s) 985
HhaI GCGC 1 cut(s) 755
Hin1I GRCGYC 2 cut(s) 167, 753
Hin1II CATG 6 cut(s) 70, 605, 667, 678, 822, 854
Hin6I GCGC 1 cut(s) 753
HinP1I GCGC 1 cut(s) 753
HinfI GANTC 2 cut(s) 76, 810
HpaII CCGG 7 cut(s) 21, 92, 217, 297, 358, 458, 954
HphI GGTGA 3 cut(s) 316, 787, 968
Hpy166II GTNNAC 1 cut(s) 233
Hpy188I TCNGA 5 cut(s) 118, 202, 840, 870, 972
Hpy188III TCNNGA 4 cut(s) 190, 565, 637, 706
Hpy8I GTNNAC 1 cut(s) 233
Hpy99I CGWCG 2 cut(s) 172, 692
HpyAV CCTTC 6 cut(s) 43, 147, 267, 607, 772, 892
HpyCH4III ACNGT 1 cut(s) 836
HpyCH4IV ACGT 1 cut(s) 167
HpyCH4V TGCA 9 cut(s) 14, 222, 251, 335, 574, 822, 845, 850, 981
HpyF10VI GCNNNNNNNGC 2 cut(s) 353, 851
HpyF3I CTNAG 3 cut(s) 281, 442, 785
HpySE526I ACGT 1 cut(s) 167
Hsp92I GRCGYC 2 cut(s) 167, 753
Hsp92II CATG 6 cut(s) 70, 605, 667, 678, 822, 854
HspAI GCGC 1 cut(s) 753
KasI GGCGCC 1 cut(s) 752
Kzo9I GATC 5 cut(s) 88, 684, 921, 958, 967
LmnI GCTCC 1 cut(s) 116
LweI GCATC 1 cut(s) 398
MaeII ACGT 1 cut(s) 167
MaeIII GTNAC 4 cut(s) 322, 659, 670, 728
MalI GATC 5 cut(s) 90, 686, 923, 960, 969
MboI GATC 5 cut(s) 88, 684, 921, 958, 967
MboII GAAGA 4 cut(s) 169, 482, 600, 721
MfeI CAATTG 1 cut(s) 395
MflI RGATCY 1 cut(s) 967
MhlI GDGCHC 2 cut(s) 113, 882
MluCI AATT 4 cut(s) 127, 395, 632, 654
MluI ACGCGT 1 cut(s) 994
Mly113I GGCGCC 1 cut(s) 753
MlyI GAGTC 1 cut(s) 819
MnlI CCTC 5 cut(s) 124, 381, 465, 526, 643
Mph1103I ATGCAT 1 cut(s) 852
MseI TTAA 2 cut(s) 429, 503
MslI CAYNNNNRTG 1 cut(s) 71
MspI CCGG 7 cut(s) 21, 92, 217, 297, 358, 458, 954
MspR9I CCNGG 3 cut(s) 297, 358, 860
MunI CAATTG 1 cut(s) 395
MvaI CCWGG 1 cut(s) 860
MvnI CGCG 1 cut(s) 996
MwoI GCNNNNNNNGC 2 cut(s) 353, 851
NarI GGCGCC 1 cut(s) 753
NciI CCSGG 2 cut(s) 297, 358
NdeII GATC 5 cut(s) 88, 684, 921, 958, 967
NlaIII CATG 6 cut(s) 70, 605, 667, 678, 822, 854
NlaIV GGNNCC 5 cut(s) 48, 112, 278, 754, 880
NmeAIII GCCGAG 1 cut(s) 781
NmuCI GTSAC 3 cut(s) 322, 670, 728
NsiI ATGCAT 1 cut(s) 852
NspI RCATGY 3 cut(s) 605, 678, 854
NspV TTCGAA 1 cut(s) 611
PaeI GCATGC 1 cut(s) 854
PciI ACATGT 1 cut(s) 601
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PfeI GAWTC 1 cut(s) 76
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 1 cut(s) 818
PluTI GGCGCC 1 cut(s) 756
PpsI GAGTC 1 cut(s) 818
PscI ACATGT 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 858
PspGI CCWGG 1 cut(s) 858
PspN4I GGNNCC 5 cut(s) 48, 112, 278, 754, 880
PspOMI GGGCCC 1 cut(s) 878
PspPI GGNCC 2 cut(s) 878, 879
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 1 cut(s) 967
RsaI GTAC 5 cut(s) 494, 516, 539, 547, 794
RsaNI GTAC 5 cut(s) 493, 515, 538, 546, 793
RseI CAYNNNNRTG 1 cut(s) 71
SaqAI TTAA 2 cut(s) 429, 503
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 5 cut(s) 88, 684, 921, 958, 967
Sau96I GGNCC 2 cut(s) 878, 879
SchI GAGTC 1 cut(s) 819
ScrFI CCNGG 3 cut(s) 297, 358, 860
SduI GDGCHC 2 cut(s) 113, 882
SfaNI GCATC 1 cut(s) 398
SfoI GGCGCC 1 cut(s) 754
SfuI TTCGAA 1 cut(s) 611
SgrAI CRCCGGYG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 71
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
SphI GCATGC 1 cut(s) 854
Sse9I AATT 4 cut(s) 127, 395, 632, 654
SsiI CCGC 1 cut(s) 345
SspDI GGCGCC 1 cut(s) 752
SspI AATATT 1 cut(s) 799
StyD4I CCNGG 3 cut(s) 295, 356, 858
StyI CCWWGG 1 cut(s) 1000
TaaI ACNGT 1 cut(s) 836
TaiI ACGT 1 cut(s) 170
TaqI TCGA 6 cut(s) 189, 597, 611, 687, 720, 924
TasI AATT 4 cut(s) 127, 395, 632, 654
TatI WGTACW 2 cut(s) 537, 545
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 2 cut(s) 429, 503
Tru9I TTAA 2 cut(s) 429, 503
TscAI CASTG 1 cut(s) 265
TseFI GTSAC 3 cut(s) 322, 670, 728
TseI GCWGC 1 cut(s) 11
Tsp45I GTSAC 3 cut(s) 322, 670, 728
TspGWI ACGGA 2 cut(s) 219, 502
TspRI CASTG 1 cut(s) 265
XapI RAATTY 2 cut(s) 127, 632
XceI RCATGY 3 cut(s) 605, 678, 854
XcmI CCANNNNNNNNNTGG 2 cut(s) 58, 265
ZraI GACGTC 1 cut(s) 168
Zsp2I ATGCAT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.