Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0004278

Physical Location & Seq

Genomic Coordinates Forward (+)

34830890 .. 34835557

Gene Structure 4668 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1216000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2910 bp

ATGGTGAGACTGAAGGTGAGGAAATCTAGCCATCTCCATTTGTTCCTGATATACTCTCCAATGCGAGTCAATGATCTTAAATTTTTCCCCGTTTTGGAATTTTATTTTGCAGGGAAGCGGAGAGCGTACTTAGTAGAGTGGTTAAATAGTTTAGTTCCTAATCTAGGTTTACCAACAAATGCTTCATACGAGGACTTGCGATCGTGCTTGATCGATGGTACCGTTTTATGTCTGATGTTGAATAGGCTTAGACCTGGTCTTGTTGATAAGAATTCGATGTCACCCTCAGAAAATGTTGCAAGGTTTCTGGCAGCTATGGATGCATTAGGGCTGCCGAAGTTTGATATGTCCGACCTAGAGAAGGGGTCTATGAAGACTGTCACAAACTGTCTTTTAACACTTAAAGCAAAACTTATGCCAAATGTTATGGGAGATGGTATTACTATAACTAGTCCGACGACTAAATCTGGCAGCCAACCTTCCCGCTTCCAGCTTTCTCAATTATCTGTAGATGAGAGGCGGAAGGTCCTGTCTGAGTCAAAATTTCAGCGCGCATTGCATAGTCCTGTTATGGCAGGTCTGGCCATTGTTGGGTTTGACGCAAATTTACCTTCAGCAGAGACATCGCCTTCACGAATGCATCATGTTGGGCATAAGTTCCATGAGGTGTTGCAGTTGAAGCAAGGAAGCTACGCGGATCTTCCTGCTGCAAAAATTTCAGAAATGATGAAACCGAACAGTTTAGATAACGCTCCAACGCAGTCACTTTTGAGTGTCGTAAATGGAATCCTCGATGAAGGTGTTGAAAGAAAGAATGGTGAAATACCTCATCGTGTGTCTTGCCTTCTGCGAAGAGTTGTCCAGGAGATTGAGCGGCGAATATCAACTCAGGCGGAGCATTTAAGAACTCAAAACAACCTTTTTAAGGCTCGTGAAGAGAAATACCAGTCAAGAATCAGAGTACTTGAAACACTTGCATCCGGAACCAGTGAGGAAAGTGAGAGTGAGAGAACCCGAATGGAAGCAAAGAAGAAAACCGATGGCGAGGATGTGAATGTGATCAGATTGATGAAAGAGAGGGATCAAGGCAACCTTGAAATTTCAGGATTGAAGCAAGAGTTGGATATAGCAAAAAAGACATATGACCTGCGTTGCTTGCAAATGGAAATGGAAGAGAAGGGTGCTAGAACGGAGCTTGAAGAGAGGATAAAAGAACTCGAACGTCTCTTGGCAGAGTCAAGGAGTAAGGTGAAAGAGCTCCAGGCAAATTCTGAGTCAAAACATCAGTCTAATAGAGCAGAACTTGAAGAGAGAGTAAAAGAACTTGAACGTTCTTTGGAAGAATCAAGGAATAAAGTGAAAGAACTCGAGGCAAGTTCCGAGTCCAAATATCAATTTTCTAAAGTAGAGCTTGAAGGGAGAATAAAAGAACTTGAACGCCTCTTGGCAGATTCAAGGAATGAGGCGAAGCAGCTTGCAACGAATTCAGAGTCAAAATATAAAAGTTGGAGCAAGAAACTGCGTGATTACGAGAACTCTATGGACCTTCAACTTGGTTCACTAAAGGAATTAAGATTATCTTCGGAGTTCATTAAGCAAGAAGTTTTGACGGCAGAACACAGTTACGCGGTGGACTTCAATCACTTAGGAGTGAAACTTCAAGCATTAGCAGACGCGTCTGAGAACTATCATGCACTTCTCGCTGAAAACAGGAAGTTATTTAACGAAATTCAGGATTTAAGAGGAAACATCAGAGTTTACTGTCGAATAAGACCATTTCTTCCTGGAGAAAAAGAAAAGAAGACGTTGGTAGAACGTATTGGTGAAAATGGGGAACTGGTAGTTGCAGACCGCTCCAAACCAGGGAAGGAGGGTCATCGATTGTTCAAGTTTAACGAGGTCTATGGTTCAGATGCAAGTCAAGGCGAAGTATACGCTGACACACAACCATTGATACGATCTGTGCTTGATGGATATAATGTATGTATTTTTGCTTACGGTCAAACAGGATCCGGGAAAACATACACGATGACTGGTCCTAATGGATCAACTAAAGAGAACTGGGGAATCAACTACCGTGCCTTGAATGACCTTTTCGACATCTCTCAACGTAGAAAAAGCTCCATTACATATGAAATTCGAGTCCAAATGGTTGAAATATACAACGAACAAGTGCACGATTTGCTATCCAGCGATGGTGCTCAGAAGAAATATCCTTCTGTGCCAGATGCTAGCATGCACCCCGTGGAAGCAACCTCAGATGTAATAAGATTGATGGGAATCGGGTTTAAGAACCGAGCTGTTGGTGCCACTGCGTTAAACGAAAGAAGTAGTCGCTCTCATAGTGTTGTTACAGTTCATGTACGTGGGAAGGATTTGAAGACTGGTTCCGCATTGATTGGTAACCTTCATCTTGTAGATCTCGCGGGAAGTGAAAGAGTGGACCGTTCGGAGGTAACAGGAGACAGGCTCAAGGAAGCACAACATATAAACAAATCATTGTCTTCGCTCGGAGATGTCATATTTGCTCTCGCACAAAAGAGCCCTCATGTACCTTACAGAAACAGCAAGCTCACTCAAGTCCTTCAAAGCTCTCTCGGTGGGCAAGCGAAAACGCTAATGTTTGTGCAGCTAAATCCCGATGCGAGTTCATTTTCCGAAAGTTTAAGTACTCTAAAGTTTGCTGAGAGGGTTGCCGGAGTTGAGTTGGGAGCTGCGAAAAGCAACAAAGAGGGCCGGGATGTAAGGGAATTAATGGAACAGGTAGCGTCCCTCAAAGACACGATTGCTAAGAAGGACGGCGAGATTGAGCGATTGCAATTACTTGATGGCCACAGTGAGAAGCGTGGGACGAGCTCATTTAGGTATGAATCTTCGCAGCCAAGCAGAGTTTCTAGAGGTGGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

970

Amino Acids

108.92

Weight (kDa)

8.93

Isoelectric Point (pI)

44.66

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	41 - 136	7e-12	Calponin homology (CH) domain
Microtub_bd	PF16796	569 - 729	1.4e-40	Microtubule binding
Kinesin	PF00225	589 - 899	4.6e-104	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G73860 AT1G73860
fragaria_vesca	FvH4_5g12130 FvH4_5g12130
malus_domestica	MD06G1216000.v1.1 MD14G1226600.v1.1
prunus_persica	Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1
pyrus_communis	pycom06g19260 pycom14g18800
rosa_chinensis	RchiOBHm_Chr7g0185441 RchiOBHm_Chr7g0185451
rosa_laevigata	RLG00000004912
rosa_multiflora	Rmu_sc0010190.1_g000028
rosa_roxburghii	Rroxscaffold_3G00268930
rosa_rugosa	Rorug06G0469100 Rorug06G0469200 Rorug06G0469300 Rorug06G0469400 Rorug06G0469500
rosa_samantha	Rh7AG073500 Rh7BG074100 Rh7CG074300 Rh7DG074200
rosa_wichuraiana	Rw7G006170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	566, 1155
Acc65I	GGTACC	1 cut(s)	218
AccB1I	GGYRCC	2 cut(s)	218, 2306
AccBSI	CCGCTC	2 cut(s)	874, 1854
AccI	GTMKAC	1 cut(s)	1932
AccII	CGCG	5 cut(s)	552, 695, 1628, 1676, 2426
AccIII	TCCGGA	1 cut(s)	980
AclI	AACGTT	1 cut(s)	1330
AclWI	GGATC	5 cut(s)	705, 1089, 2004, 2017, 2053
AcoI	YGGCCR	2 cut(s)	582, 2830
AcuI	CTGAAG	2 cut(s)	32, 597
AdeI	CACNNNGTG	1 cut(s)	2245
AfaI	GTAC	6 cut(s)	128, 220, 963, 2364, 2553, 2671
AfiI	CCNNNNNNNGG	7 cut(s)	94, 164, 361, 591, 925, 1863, 2452
AflIII	ACRYGT	1 cut(s)	1674
AhlI	ACTAGT	1 cut(s)	449
AjnI	CCWGG	5 cut(s)	253, 861, 1260, 1783, 1861
AjuI	GAANNNNNNNTTGG	6 cut(s)	166, 198, 1103, 1135, 2875, 2907
Alw21I	GWGCWC	4 cut(s)	1260, 2178, 2203, 2858
Alw26I	GTCTC	3 cut(s)	614, 1229, 2457
Alw44I	GTGCAC	1 cut(s)	2174
AlwI	GGATC	5 cut(s)	705, 1089, 2004, 2017, 2053
Ama87I	CYCGRG	1 cut(s)	1367
Aor13HI	TCCGGA	1 cut(s)	980
AoxI	GGCC	3 cut(s)	582, 2734, 2830
ApaLI	GTGCAC	1 cut(s)	2174
ApeKI	GCWGC	8 cut(s)	311, 331, 471, 707, 1471, 2629, 2714, 2878
ArsI	GACNNNNNNTTYG	6 cut(s)	1259, 1271, 1291, 1303, 1475, 1507
AseI	ATTAAT	1 cut(s)	2753
Asp718I	GGTACC	1 cut(s)	218
AspLEI	GCGC	2 cut(s)	552, 554
AspS9I	GGNCC	5 cut(s)	526, 1543, 2036, 2443, 2734
AsuC2I	CCSGG	2 cut(s)	2014, 2738
AsuHPI	GGTGA	6 cut(s)	16, 28, 273, 830, 1261, 1835
AsuNHI	GCTAGC	1 cut(s)	2231
AvaI	CYCGRG	1 cut(s)	1367
AvaII	GGWCC	4 cut(s)	526, 1543, 2036, 2443
BaeGI	GKGCMC	1 cut(s)	2178
BalI	TGGCCA	2 cut(s)	584, 2832
BamHI	GGATCC	1 cut(s)	2009
BanI	GGYRCC	2 cut(s)	218, 2306
BanII	GRGCYC	3 cut(s)	1260, 2546, 2858
BauI	CACGAG	1 cut(s)	930
BbsI	GAAGAC	4 cut(s)	380, 1808, 2387, 2496
Bbv12I	GWGCWC	4 cut(s)	1260, 2178, 2203, 2858
BbvI	GCAGC	8 cut(s)	318, 323, 483, 694, 1483, 2641, 2701, 2890
BccI	CCATC	8 cut(s)	39, 209, 428, 1034, 1964, 2189, 2269, 2822
BceAI	ACGGC	2 cut(s)	1626, 2815
BcgI	CGANNNNNNTGC	2 cut(s)	606, 640
BciT130I	CCWGG	5 cut(s)	255, 863, 1262, 1785, 1863
BclI	TGATCA	1 cut(s)	1059
BcnI	CCSGG	2 cut(s)	2014, 2738
BcoDI	GTCTC	3 cut(s)	614, 1229, 2457
BcuI	ACTAGT	1 cut(s)	449
BfaI	CTAG	7 cut(s)	27, 164, 356, 450, 1185, 2232, 2895
BfmI	CTRYAG	1 cut(s)	507
BfuAI	ACCTGC	2 cut(s)	566, 1155
BglII	AGATCT	1 cut(s)	2419
BisI	GCNGC	9 cut(s)	312, 332, 472, 708, 875, 1472, 2630, 2715, 2879
BlsI	GCNGC	9 cut(s)	313, 333, 473, 709, 876, 1473, 2631, 2716, 2880
BmcAI	AGTACT	2 cut(s)	963, 2671
Bme1390I	CCNGG	7 cut(s)	255, 863, 1262, 1785, 1863, 2014, 2738
Bme18I	GGWCC	4 cut(s)	526, 1543, 2036, 2443
BmeT110I	CYCGRG	1 cut(s)	1367
BmgT120I	GGNCC	5 cut(s)	526, 1543, 2036, 2443, 2734
BmiI	GGNNCC	5 cut(s)	220, 985, 2011, 2308, 2389
BmrFI	CCNGG	7 cut(s)	255, 863, 1262, 1785, 1863, 2014, 2738
BmrI	ACTGGG	1 cut(s)	2071
BmsI	GCATC	6 cut(s)	310, 649, 986, 1903, 2218, 2632
BmtI	GCTAGC	1 cut(s)	2235
BmuI	ACTGGG	1 cut(s)	2071
BpiI	GAAGAC	4 cut(s)	380, 1808, 2387, 2496
BplI	GAGNNNNNCTC	2 cut(s)	2454, 2486
BpmI	CTGGAG	2 cut(s)	1244, 1806
BpuEI	CTTGAG	2 cut(s)	2456, 2562
BpuMI	CCSGG	2 cut(s)	2014, 2738
Bsa29I	ATCGAT	2 cut(s)	213, 1879
BsaAI	YACGTR	1 cut(s)	2366
BsaBI	GATNNNNATC	1 cut(s)	2279
BsaJI	CCNNGG	2 cut(s)	1862, 2244
BsaWI	WCCGGW	1 cut(s)	980
Bsc4I	CCNNNNNNNGG	7 cut(s)	94, 164, 361, 591, 925, 1863, 2452
Bse1I	ACTGG	6 cut(s)	946, 987, 1842, 2038, 2066, 2389
Bse3DI	GCAATG	1 cut(s)	554
Bse8I	GATNNNNATC	1 cut(s)	2279
BseAI	TCCGGA	1 cut(s)	980
BseBI	CCWGG	5 cut(s)	255, 863, 1262, 1785, 1863
BseCI	ATCGAT	2 cut(s)	213, 1879
BseDI	CCNNGG	2 cut(s)	1862, 2244
BseGI	GGATG	4 cut(s)	325, 977, 1054, 2746
BseJI	GATNNNNATC	1 cut(s)	2279
BseLI	CCNNNNNNNGG	7 cut(s)	94, 164, 361, 591, 925, 1863, 2452
BseMI	GCAATG	1 cut(s)	554
BseMII	CTCAG	8 cut(s)	300, 525, 902, 1263, 1671, 2216, 2271, 2676
BseNI	ACTGG	6 cut(s)	946, 987, 1842, 2038, 2066, 2389
BsePI	GCGCGC	1 cut(s)	550
BseSI	GKGCMC	1 cut(s)	2178
BseXI	GCAGC	8 cut(s)	318, 323, 483, 694, 1483, 2641, 2701, 2890
BsgI	GTGCAG	1 cut(s)	2648
Bsh1236I	CGCG	5 cut(s)	552, 695, 1628, 1676, 2426
Bsh1285I	CGRYCG	1 cut(s)	203
BshFI	GGCC	3 cut(s)	584, 2736, 2832
BshNI	GGYRCC	2 cut(s)	218, 2306
BshVI	ATCGAT	2 cut(s)	213, 1879
BsiEI	CGRYCG	1 cut(s)	203
BsiHKAI	GWGCWC	4 cut(s)	1260, 2178, 2203, 2858
BsiHKCI	CYCGRG	1 cut(s)	1367
BsiSI	CCGG	4 cut(s)	981, 2013, 2697, 2737
BslFI	GGGAC	2 cut(s)	2755, 2863
BslI	CCNNNNNNNGG	7 cut(s)	94, 164, 361, 591, 925, 1863, 2452
BsmAI	GTCTC	3 cut(s)	614, 1229, 2457
BsmBI	CGTCTC	1 cut(s)	1229
BsmFI	GGGAC	2 cut(s)	2755, 2863
BsmI	GAATGC	1 cut(s)	642
BsnI	GGCC	3 cut(s)	584, 2736, 2832
BsoBI	CYCGRG	1 cut(s)	1367
Bsp1286I	GDGCHC	5 cut(s)	1260, 2178, 2203, 2546, 2858
Bsp13I	TCCGGA	1 cut(s)	980
BspANI	GGCC	3 cut(s)	584, 2736, 2832
BspCNI	CTCAG	8 cut(s)	299, 526, 901, 1264, 1672, 2215, 2270, 2677
BspDI	ATCGAT	2 cut(s)	213, 1879
BspEI	TCCGGA	1 cut(s)	980
BspFNI	CGCG	5 cut(s)	552, 695, 1628, 1676, 2426
BspLI	GGNNCC	5 cut(s)	220, 985, 2011, 2308, 2389
BspMI	ACCTGC	2 cut(s)	566, 1155
BspOI	GCTAGC	1 cut(s)	2235
BspPI	GGATC	5 cut(s)	705, 1089, 2004, 2017, 2053
BspT107I	GGYRCC	2 cut(s)	218, 2306
BsrBI	CCGCTC	2 cut(s)	874, 1854
BsrDI	GCAATG	1 cut(s)	554
BsrI	ACTGG	6 cut(s)	946, 987, 1842, 2038, 2066, 2389
BssECI	CCNNGG	2 cut(s)	1862, 2244
BssHII	GCGCGC	1 cut(s)	550
BssNAI	GTATAC	1 cut(s)	1933
BssSI	CACGAG	1 cut(s)	930
Bst1107I	GTATAC	1 cut(s)	1933
Bst2BI	CACGAG	1 cut(s)	930
Bst2UI	CCWGG	5 cut(s)	255, 863, 1262, 1785, 1863
Bst6I	CTCTTC	5 cut(s)	847, 930, 1167, 1194, 1302
BstAPI	GCANNNNNTGC	1 cut(s)	2182
BstBAI	YACGTR	1 cut(s)	2366
BstC8I	GCNNGC	7 cut(s)	552, 1157, 1476, 2233, 2237, 2570, 2607
BstDSI	CCRYGG	1 cut(s)	2244
BstEII	GGTNACC	1 cut(s)	2402
BstENI	CCTNNNNNAGG	3 cut(s)	162, 359, 923
BstF5I	GGATG	4 cut(s)	325, 977, 1054, 2746
BstFNI	CGCG	5 cut(s)	552, 695, 1628, 1676, 2426
BstHHI	GCGC	2 cut(s)	552, 554
BstMAI	GTCTC	3 cut(s)	614, 1229, 2457
BstMCI	CGRYCG	1 cut(s)	203
BstMWI	GCNNNNNNNGC	8 cut(s)	320, 556, 581, 679, 1156, 1700, 2182, 2306
BstNI	CCWGG	5 cut(s)	255, 863, 1262, 1785, 1863
BstNSI	RCATGY	1 cut(s)	2239
BstPI	GGTNACC	1 cut(s)	2402
BstSCI	CCNGG	7 cut(s)	253, 861, 1260, 1783, 1861, 2012, 2736
BstSFI	CTRYAG	1 cut(s)	507
BstSLI	GKGCMC	1 cut(s)	2178
BstUI	CGCG	5 cut(s)	552, 695, 1628, 1676, 2426
BstV1I	GCAGC	8 cut(s)	318, 323, 483, 694, 1483, 2641, 2701, 2890
BstV2I	GAAGAC	4 cut(s)	380, 1808, 2387, 2496
BstX2I	RGATCY	3 cut(s)	697, 2009, 2419
BstYI	RGATCY	3 cut(s)	697, 2009, 2419
BstZ17I	GTATAC	1 cut(s)	1933
Bsu15I	ATCGAT	2 cut(s)	213, 1879
BsuRI	GGCC	3 cut(s)	584, 2736, 2832
BsuTUI	ATCGAT	2 cut(s)	213, 1879
BtgI	CCRYGG	1 cut(s)	2244
BtgZI	GCGATG	2 cut(s)	609, 2208
BtsCI	GGATG	4 cut(s)	325, 977, 1054, 2746
BtsI	GCAGTG	1 cut(s)	2310
BtsIMutI	CAGTG	3 cut(s)	994, 2310, 2842
BveI	ACCTGC	2 cut(s)	566, 1155
Cac8I	GCNNGC	7 cut(s)	552, 1157, 1476, 2233, 2237, 2570, 2607
CfoI	GCGC	2 cut(s)	552, 554
Cfr13I	GGNCC	5 cut(s)	526, 1543, 2036, 2443, 2734
ClaI	ATCGAT	2 cut(s)	213, 1879
CseI	GACGC	4 cut(s)	608, 1665, 1682, 2757
CsiI	ACCWGGT	1 cut(s)	253
Csp6I	GTAC	6 cut(s)	127, 219, 962, 2363, 2552, 2670
CviAII	CATG	6 cut(s)	644, 662, 1691, 2236, 2360, 2549
CviQI	GTAC	6 cut(s)	127, 219, 962, 2363, 2552, 2670
DraIII	CACNNNGTG	1 cut(s)	2245
EaeI	YGGCCR	2 cut(s)	582, 2830
Eam1104I	CTCTTC	5 cut(s)	847, 930, 1167, 1194, 1302
EarI	CTCTTC	5 cut(s)	847, 930, 1167, 1194, 1302
EciI	GGCGGA	2 cut(s)	535, 908
Ecl136II	GAGCTC	2 cut(s)	1258, 2856
Eco24I	GRGCYC	3 cut(s)	1260, 2546, 2858
Eco47I	GGWCC	4 cut(s)	526, 1543, 2036, 2443
Eco53kI	GAGCTC	2 cut(s)	1258, 2856
Eco57I	CTGAAG	2 cut(s)	32, 597
Eco88I	CYCGRG	1 cut(s)	1367
Eco91I	GGTNACC	1 cut(s)	2402
EcoICRI	GAGCTC	2 cut(s)	1258, 2856
EcoNI	CCTNNNNNAGG	3 cut(s)	162, 359, 923
EcoO109I	RGGNCCY	1 cut(s)	526
EcoO65I	GGTNACC	1 cut(s)	2402
EcoRI	GAATTC	2 cut(s)	271, 1483
EcoRII	CCWGG	5 cut(s)	253, 861, 1260, 1783, 1861
EcoT22I	ATGCAT	2 cut(s)	325, 642
EcoT38I	GRGCYC	3 cut(s)	1260, 2546, 2858
Esp3I	CGTCTC	1 cut(s)	1229
FaeI	CATG	6 cut(s)	647, 665, 1694, 2239, 2363, 2552
FalI	AAGNNNNNCTT	8 cut(s)	396, 428, 1077, 1109, 1395, 1427, 1564, 1596
FaqI	GGGAC	2 cut(s)	2755, 2863
FatI	CATG	6 cut(s)	643, 661, 1690, 2235, 2359, 2548
FauI	CCCGC	2 cut(s)	491, 2419
FauNDI	CATATG	2 cut(s)	1141, 2131
FbaI	TGATCA	1 cut(s)	1059
FblI	GTMKAC	1 cut(s)	1932
Fnu4HI	GCNGC	9 cut(s)	312, 332, 472, 708, 875, 1472, 2630, 2715, 2879
FokI	GGATG	4 cut(s)	332, 964, 1061, 2753
FriOI	GRGCYC	3 cut(s)	1260, 2546, 2858
Fsp4HI	GCNGC	9 cut(s)	312, 332, 472, 708, 875, 1472, 2630, 2715, 2879
FspBI	CTAG	7 cut(s)	27, 164, 356, 450, 1185, 2232, 2895
GlaI	GCGC	2 cut(s)	551, 553
GluI	GCNGC	9 cut(s)	312, 332, 472, 708, 875, 1472, 2630, 2715, 2879
GsuI	CTGGAG	2 cut(s)	1244, 1806
HaeIII	GGCC	3 cut(s)	584, 2736, 2832
HapII	CCGG	4 cut(s)	981, 2013, 2697, 2737
HgaI	GACGC	4 cut(s)	608, 1665, 1682, 2757
HhaI	GCGC	2 cut(s)	552, 554
Hin1II	CATG	6 cut(s)	647, 665, 1694, 2239, 2363, 2552
Hin6I	GCGC	2 cut(s)	550, 552
HinP1I	GCGC	2 cut(s)	550, 552
HpaII	CCGG	4 cut(s)	981, 2013, 2697, 2737
HphI	GGTGA	6 cut(s)	16, 28, 273, 830, 1261, 1835
Hpy166II	GTNNAC	7 cut(s)	170, 1559, 1633, 1759, 1933, 2176, 2443
Hpy188III	TCNNGA	9 cut(s)	46, 633, 932, 951, 981, 1104, 1733, 2639, 2895
Hpy8I	GTNNAC	7 cut(s)	170, 1559, 1633, 1759, 1933, 2176, 2443
Hpy99I	CGWCG	1 cut(s)	460
HpyCH4IV	ACGT	6 cut(s)	1222, 1330, 1805, 1816, 2110, 2365
HpyF10VI	GCNNNNNNNGC	8 cut(s)	320, 556, 581, 679, 1156, 1700, 2182, 2306
HpySE526I	ACGT	6 cut(s)	1222, 1330, 1805, 1816, 2110, 2365
Hsp92II	CATG	6 cut(s)	647, 665, 1694, 2239, 2363, 2552
HspAI	GCGC	2 cut(s)	550, 552
Kpn2I	TCCGGA	1 cut(s)	980
KpnI	GGTACC	1 cut(s)	222
Ksp22I	TGATCA	1 cut(s)	1059
LmnI	GCTCC	8 cut(s)	757, 895, 1192, 1263, 1509, 1859, 2126, 2711
Lsp1109I	GCAGC	8 cut(s)	318, 323, 483, 694, 1483, 2641, 2701, 2890
LweI	GCATC	6 cut(s)	310, 649, 986, 1903, 2218, 2632
MabI	ACCWGGT	1 cut(s)	253
MaeI	CTAG	7 cut(s)	27, 164, 356, 450, 1185, 2232, 2895
MaeII	ACGT	6 cut(s)	1222, 1330, 1805, 1816, 2110, 2365
MaeIII	GTNAC	7 cut(s)	279, 379, 762, 1622, 2350, 2402, 2455
MbiI	CCGCTC	2 cut(s)	874, 1854
MflI	RGATCY	3 cut(s)	697, 2009, 2419
MhlI	GDGCHC	5 cut(s)	1260, 2178, 2203, 2546, 2858
MlsI	TGGCCA	2 cut(s)	584, 2832
MluI	ACGCGT	1 cut(s)	1674
MluNI	TGGCCA	2 cut(s)	584, 2832
MlyI	GAGTC	7 cut(s)	75, 545, 1244, 1283, 1391, 1499, 2151
MmeI	TCCRAC	5 cut(s)	375, 479, 779, 1101, 1487
Mox20I	TGGCCA	2 cut(s)	584, 2832
Mph1103I	ATGCAT	2 cut(s)	325, 642
MroI	TCCGGA	1 cut(s)	980
MscI	TGGCCA	2 cut(s)	584, 2832
MslI	CAYNNNNRTG	1 cut(s)	2364
Msp20I	TGGCCA	2 cut(s)	584, 2832
MspI	CCGG	4 cut(s)	981, 2013, 2697, 2737
MspR9I	CCNGG	7 cut(s)	255, 863, 1262, 1785, 1863, 2014, 2738
Mva1269I	GAATGC	1 cut(s)	642
MvaI	CCWGG	5 cut(s)	255, 863, 1262, 1785, 1863
MvnI	CGCG	5 cut(s)	552, 695, 1628, 1676, 2426
MwoI	GCNNNNNNNGC	8 cut(s)	320, 556, 581, 679, 1156, 1700, 2182, 2306
NciI	CCSGG	2 cut(s)	2014, 2738
NdeI	CATATG	2 cut(s)	1141, 2131
NheI	GCTAGC	1 cut(s)	2231
NlaIII	CATG	6 cut(s)	647, 665, 1694, 2239, 2363, 2552
NlaIV	GGNNCC	5 cut(s)	220, 985, 2011, 2308, 2389
NmuCI	GTSAC	3 cut(s)	279, 379, 762
NsiI	ATGCAT	2 cut(s)	325, 642
NspI	RCATGY	1 cut(s)	2239
PaeI	GCATGC	1 cut(s)	2239
PaeR7I	CTCGAG	1 cut(s)	1367
PauI	GCGCGC	1 cut(s)	550
PcsI	WCGNNNNNNNCGW	1 cut(s)	219
PctI	GAATGC	1 cut(s)	642
PfeI	GAWTC	7 cut(s)	786, 954, 1343, 1451, 2067, 2280, 2870
PflFI	GACNNNGTC	1 cut(s)	255
PfoI	TCCNGGA	3 cut(s)	861, 1783, 2012
PkrI	GCNGC	9 cut(s)	313, 333, 473, 709, 876, 1473, 2631, 2716, 2880
Ple19I	CGATCG	1 cut(s)	203
PleI	GAGTC	7 cut(s)	74, 544, 1243, 1282, 1390, 1498, 2150
PpsI	GAGTC	7 cut(s)	74, 544, 1243, 1282, 1390, 1498, 2150
Ppu21I	YACGTR	1 cut(s)	2366
PpuMI	RGGWCCY	1 cut(s)	526
PshBI	ATTAAT	1 cut(s)	2753
Psp124BI	GAGCTC	2 cut(s)	1260, 2858
Psp1406I	AACGTT	1 cut(s)	1330
Psp5II	RGGWCCY	1 cut(s)	526
Psp6I	CCWGG	5 cut(s)	253, 861, 1260, 1783, 1861
PspEI	GGTNACC	1 cut(s)	2402
PspGI	CCWGG	5 cut(s)	253, 861, 1260, 1783, 1861
PspN4I	GGNNCC	5 cut(s)	220, 985, 2011, 2308, 2389
PspPI	GGNCC	5 cut(s)	526, 1543, 2036, 2443, 2734
PspPPI	RGGWCCY	1 cut(s)	526
PspXI	VCTCGAGB	1 cut(s)	1367
PsuI	RGATCY	3 cut(s)	697, 2009, 2419
PsyI	GACNNNGTC	1 cut(s)	255
PteI	GCGCGC	1 cut(s)	550
PvuI	CGATCG	1 cut(s)	203
RsaI	GTAC	6 cut(s)	128, 220, 963, 2364, 2553, 2671
RsaNI	GTAC	6 cut(s)	127, 219, 962, 2363, 2552, 2670
RseI	CAYNNNNRTG	1 cut(s)	2364
SacI	GAGCTC	2 cut(s)	1260, 2858
SatI	GCNGC	9 cut(s)	312, 332, 472, 708, 875, 1472, 2630, 2715, 2879
Sau96I	GGNCC	5 cut(s)	526, 1543, 2036, 2443, 2734
ScaI	AGTACT	2 cut(s)	963, 2671
SchI	GAGTC	7 cut(s)	75, 545, 1244, 1283, 1391, 1499, 2151
ScrFI	CCNGG	7 cut(s)	255, 863, 1262, 1785, 1863, 2014, 2738
SduI	GDGCHC	5 cut(s)	1260, 2178, 2203, 2546, 2858
SexAI	ACCWGGT	1 cut(s)	253
SfaNI	GCATC	6 cut(s)	310, 649, 986, 1903, 2218, 2632
SfcI	CTRYAG	1 cut(s)	507
Sfr274I	CTCGAG	1 cut(s)	1367
SinI	GGWCC	4 cut(s)	526, 1543, 2036, 2443
SlaI	CTCGAG	1 cut(s)	1367
SmiMI	CAYNNNNRTG	1 cut(s)	2364
SmlI	CTYRAG	3 cut(s)	1367, 2471, 2577
SmoI	CTYRAG	3 cut(s)	1367, 2471, 2577
SpeI	ACTAGT	1 cut(s)	449
SphI	GCATGC	1 cut(s)	2239
SspMI	CTAG	7 cut(s)	27, 164, 356, 450, 1185, 2232, 2895
SstI	GAGCTC	2 cut(s)	1260, 2858
StyD4I	CCNGG	7 cut(s)	253, 861, 1260, 1783, 1861, 2012, 2736
TaiI	ACGT	6 cut(s)	1225, 1333, 1808, 1819, 2113, 2368
TaqI	TCGA	9 cut(s)	213, 275, 792, 1218, 1368, 1765, 1879, 2097, 2140
TatI	WGTACW	2 cut(s)	961, 2669
TauI	GCSGC	1 cut(s)	877
TfiI	GAWTC	7 cut(s)	786, 954, 1343, 1451, 2067, 2280, 2870
TscAI	CASTG	3 cut(s)	994, 2317, 2842
TseFI	GTSAC	3 cut(s)	279, 379, 762
TseI	GCWGC	8 cut(s)	311, 331, 471, 707, 1471, 2629, 2714, 2878
Tsp45I	GTSAC	3 cut(s)	279, 379, 762
TspGWI	ACGGA	1 cut(s)	1205
TspRI	CASTG	3 cut(s)	994, 2317, 2842
Tth111I	GACNNNGTC	1 cut(s)	255
VneI	GTGCAC	1 cut(s)	2174
VpaK11BI	GGWCC	4 cut(s)	526, 1543, 2036, 2443
VspI	ATTAAT	1 cut(s)	2753
XagI	CCTNNNNNAGG	3 cut(s)	162, 359, 923
XbaI	TCTAGA	1 cut(s)	2894
XceI	RCATGY	1 cut(s)	2239
XhoI	CTCGAG	1 cut(s)	1367
XmiI	GTMKAC	1 cut(s)	1932
XspI	CTAG	7 cut(s)	27, 164, 356, 450, 1185, 2232, 2895
ZrmI	AGTACT	2 cut(s)	963, 2671
Zsp2I	ATGCAT	2 cut(s)	325, 642

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.