Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0004278

Physical Location & Seq

Genomic Coordinates Forward (+)

30791471 .. 30797055

Gene Structure 5585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1226600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2916 bp

ATGGATTCCGCATCCAACTACAGCAGAAAAGAAAATGGAGGAACCAGCAGCTCAAGTGTTCCTGCTACCGGGAATAGTTTCGAACAAGTTGTAAATGGTAATGATGAGACTGAAGGCAAGCGGCGAGCATACTTAGTAGAATGGTTAAATAGTTTAGTTCCTAATCTAGGTTTACCAACAGCTGCTTCTGATGAGGACTTGAGATCGTGCTTGATCGATGGTACTGTTTTATGTTGGATATTGAACAAGCTTAGACCTGGTCTTGTTGATGAGAACTTGGTTCCACCCTCGGAAAAAGTTGCAAGGTTTCTGGCAGCTATGGATGCGCTAGGGCTGCCGAAGTTTGATATGTCCGACCTAGAGAAGGGTTGTATGAAGGCTGTCACAGAATGTCTTTTAACACTTAAAGCACAATTTATGCCAAATGTCATGGGTTATGGTGTATCTATAACTAGTCCGACGACTAAATCTGGCAGCCAATCTTCCCGTGTCCAGCTTCCTCCATTATCTGTAGATGACATGCGGAAGGTCTTGTCTGAGTCAAAATTTCATCGCGCATTGCGTAGTCCTGTTATGTCAGGAGAGACGTCACCTTTACTCATGCATCATGTCGGACATAAGTTCCACGAGGTGTTTCAGTTGAAGCAAGGAGGCTACGCGGATCTTCCTGCTGCGAAAATTTCAGAAATGATGAAACCAAACAGTTTAGATAACGCTCCAACGCAGTCACTTTTGAGTGTCGTAAATGGAATCCTCGATGAAAGTGTTGAAAGAAAGAATGGTGAAATACCCCAGCGTGTGTCTTGTCTTCTGCGAAAAGTTGTCCAGGAGATTGAGCGGCGAATATCAACTCAAGCGGAGCATTTAAGAACTCAAAACAACCTTTTTAAGGCTCGTGAAGAGAAATACCAGTCAAGAATCCGAGTACTTGAAACACTTGCATCCGGAACCAGTGAGGAAAGTGAGCTTATCATGAACCATCTTCAGCAAATAAAGAATGAGAGAACCCGAATGGAAGCAAAGAAGAAAACCGATGATGAGGATGTGATCAGATTAATGAAAGAGAAGGATCAATGCAACCTTGAAATTTCAGGATTGAGGCAAGAGTTGGATATAGCGAAAAAGACATATGACCTGCGTTGCTTGCAAATGGAAACGGAAGCAAAAGGTGCTAGAGCGGAGCTTGAAGAGAGGACAAAAGAACTTGAACGTCTCTTGGCAGCGTCGAGGAGTAAGGTGAAGGAGCTCCAGGAAAATTCCGAGTCAAAACATCAGTCTAATAAATCAGAACTTGAAGAGAGAGTAAAAGAACTTGAACGTTCTTTGGCAGAATCAAGGAATAAAGTGAAAGAACTCGAGGCAAGTTCCGAGTCCAAATATCAGTTTTCTAAAGCAGAGCTAGAAGGGAGAATAAAAGAACTTGAACGCCTCTTGGCAGATTCAAGGAATGAGGCGAAGCAGCTTTCAGCGAATTCAGAGTCGAAATATAAAAGTTGGACCAAGAAACTGCGTGTTTACCAGAGCTCTATGGATCTTCAACTTGGTTCACTAAAGGAATTAAGGTTATCTTCAGAATCCATTAAGCAAGAAGTTTTGACGGCAGGACAGAATTACGCGGTGAACTTTAATCACTTAGGAGTGAAGCTTCAAGCATTAGCAGACGCGTCCGAGAACTATCATGCACTTCTCGCTGAAAATAGGAAGTTATTTAATGAAATTCAGGATTTAAGAGGAAACATCAGAGTCTTCTGTCGAATAAGACCATTTCTGCCTGGACAAAAGGAAAAGAAGACATCGGTAGAACATATTGGTGAAAATGGGGAACTGGTAGTTGCAGACCGCTCCAAACCAGGGAAAGAGGGTCATCGATTGTTCAAGTTTAATAAGGTCTATGGTTCAGATGCAACTCAAGCTGAAGTATACGCTGACACACAACCGTTGATACGATCTGTGCTTGATGGATACAATGTTTGTATTTGTGCTTATGGTCAAACTGGATCCGGGAAAACCTACACGATGACTGGTCCTAATCAAGCAACTAAAGAGAACTGGGGGGTCAACTACCGGGCCTTAAACGACCTTTTTGACATCTCTCAATGTAGAAAAGGCTCCATTACATATGAAATTGGAGTCCAAATGGTTGAAATATACAACGAACAGTCTTTTGTTTGCCTTGACTTGCACACACTTGGGATTATGACCCACTCTCAACCCAACGGGTTAGCTGTGCCAGATGCTAGCATGCACCCCGTGGAATCAACCTCAGATGTAATAAAATTGATGGGAATTGGGTTTAAGAACCGAGCTGTTAGTGCCACTGCTTTAAACGAAAGAAGTAGTCGCTCTCATAGTGTTGTTACAGTACATGTACGTGGGAAGGATTTGAAGACTGGTTCCGCTTTGATTGGTAACCTTCATCTTGTAGATCTCGCGGGAAGTGAAAGAGTAGACCGTTCTGAGGTAACAGGAGATAGGCTCAAGGAAGCACAACATATAAACAAATCATTATCTTCTCTTGGAGATGTCATATTTGCTCTTGCACAAAAGAGCCCTCATGTACCTTACAGAAATAGCAAGCTCACTCAAGTCCTTCAAAGCTCTCTCGGTGGGCAAGCAAAAACGCTTATGTTTGTGCAGCTAAATCCTGACGCAAGTTCATTTTCCGAAAGTTTAAGTACTCTAAAGTTTGCTGAGAGGGCTGCCGGAGTTGAGTTGGGGGCTGCAAAAAGCAACAAAGAGGGCCGGGATGTTAGGGAATTAATGGAACAGGTAGCGTCTCTCAAAGACACGATTGCTAAGAAGGATGGCGAGATTGAGCGATTGCAATTACTTGATGGCCACAGTGAGAAGCGTGGGACGAGCTCATTTAGGTATGAATCTTCGCAACCAAGCAGAGTTTCTAGAGGTGGGAAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

972

Amino Acids

108.11

Weight (kDa)

8.43

Isoelectric Point (pI)

46.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	42 - 139	1.5e-09	Calponin homology (CH) domain
Microtub_bd	PF16796	565 - 721	1.6e-37	Microtubule binding
Kinesin	PF00225	585 - 901	1.7e-100	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G73860 AT1G73860
fragaria_vesca	FvH4_5g12130 FvH4_5g12130
malus_domestica	MD06G1216000.v1.1 MD14G1226600.v1.1
prunus_persica	Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1
pyrus_communis	pycom06g19260 pycom14g18800
rosa_chinensis	RchiOBHm_Chr7g0185441 RchiOBHm_Chr7g0185451
rosa_laevigata	RLG00000004912
rosa_multiflora	Rmu_sc0010190.1_g000028
rosa_roxburghii	Rroxscaffold_3G00268930
rosa_rugosa	Rorug06G0469100 Rorug06G0469200 Rorug06G0469300 Rorug06G0469400 Rorug06G0469500
rosa_samantha	Rh7AG073500 Rh7BG074100 Rh7CG074300 Rh7DG074200
rosa_wichuraiana	Rw7G006170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	590
Acc36I	ACCTGC	1 cut(s)	1143
AccBSI	CCGCTC	3 cut(s)	838, 1178, 1842
AccI	GTMKAC	2 cut(s)	1920, 2448
AccII	CGCG	5 cut(s)	555, 659, 1616, 1664, 2432
AccIII	TCCGGA	1 cut(s)	944
AclI	AACGTT	1 cut(s)	1318
AclWI	GGATC	5 cut(s)	669, 1077, 1539, 1992, 2005
AcoI	YGGCCR	1 cut(s)	2836
AcsI	RAATTY	6 cut(s)	545, 678, 1086, 1255, 1471, 1716
AcuI	CTGAAG	4 cut(s)	132, 968, 1554, 1935
AcyI	GRCGYC	1 cut(s)	587
AdeI	CACNNNGTG	2 cut(s)	631, 2251
AfaI	GTAC	6 cut(s)	223, 927, 2364, 2370, 2559, 2677
AfiI	CCNNNNNNNGG	6 cut(s)	68, 167, 364, 889, 1851, 2458
AflIII	ACRYGT	2 cut(s)	1662, 2365
AhlI	ACTAGT	1 cut(s)	452
AjnI	CCWGG	5 cut(s)	256, 825, 1248, 1771, 1849
AjuI	GAANNNNNNNTTGG	4 cut(s)	169, 201, 2881, 2913
Alw21I	GWGCWC	3 cut(s)	1248, 1526, 2864
Alw26I	GTCTC	4 cut(s)	101, 578, 1217, 2781
AlwI	GGATC	5 cut(s)	669, 1077, 1539, 1992, 2005
Ama87I	CYCGRG	1 cut(s)	1355
Aor13HI	TCCGGA	1 cut(s)	944
AoxI	GGCC	3 cut(s)	2067, 2740, 2836
ApoI	RAATTY	6 cut(s)	545, 678, 1086, 1255, 1471, 1716
ArsI	GACNNNNNNTTYG	4 cut(s)	1259, 1291, 1463, 1495
AseI	ATTAAT	2 cut(s)	1055, 2759
Asp700I	GAANNNNTTC	1 cut(s)	77
AspLEI	GCGC	2 cut(s)	328, 557
AspS9I	GGNCC	4 cut(s)	1497, 2024, 2067, 2740
AsuC2I	CCSGG	4 cut(s)	70, 2002, 2066, 2744
AsuHPI	GGTGA	5 cut(s)	582, 794, 1249, 1630, 1823
AsuII	TTCGAA	1 cut(s)	81
AsuNHI	GCTAGC	1 cut(s)	2237
AvaI	CYCGRG	1 cut(s)	1355
AvaII	GGWCC	2 cut(s)	1497, 2024
BalI	TGGCCA	1 cut(s)	2838
BamHI	GGATCC	1 cut(s)	1997
BanII	GRGCYC	4 cut(s)	1248, 1526, 2552, 2864
BauI	CACGAG	2 cut(s)	626, 894
BbsI	GAAGAC	4 cut(s)	800, 1738, 1796, 2393
Bbv12I	GWGCWC	3 cut(s)	1248, 1526, 2864
BccI	CCATC	6 cut(s)	212, 987, 1952, 2275, 2798, 2828
BceAI	ACGGC	1 cut(s)	1614
BciT130I	CCWGG	5 cut(s)	258, 827, 1250, 1773, 1851
BciVI	GTATCC	1 cut(s)	1955
BclI	TGATCA	1 cut(s)	1047
BcnI	CCSGG	4 cut(s)	70, 2002, 2066, 2744
BcoDI	GTCTC	4 cut(s)	101, 578, 1217, 2781
BcuI	ACTAGT	1 cut(s)	452
BfaI	CTAG	8 cut(s)	167, 329, 359, 453, 1173, 1400, 2238, 2901
BfmI	CTRYAG	2 cut(s)	19, 510
BfuAI	ACCTGC	1 cut(s)	1143
BfuI	GTATCC	1 cut(s)	1955
BglII	AGATCT	1 cut(s)	2425
BmcAI	AGTACT	2 cut(s)	927, 2677
Bme1390I	CCNGG	9 cut(s)	70, 258, 827, 1250, 1773, 1851, 2002, 2066, 2744
Bme18I	GGWCC	2 cut(s)	1497, 2024
BmeT110I	CYCGRG	1 cut(s)	1355
BmgT120I	GGNCC	4 cut(s)	1497, 2024, 2067, 2740
BmiI	GGNNCC	6 cut(s)	43, 282, 949, 1999, 2110, 2395
BmrFI	CCNGG	9 cut(s)	70, 258, 827, 1250, 1773, 1851, 2002, 2066, 2744
BmrI	ACTGGG	1 cut(s)	2059
BmsI	GCATC	6 cut(s)	20, 313, 613, 950, 1891, 2224
BmtI	GCTAGC	1 cut(s)	2241
BmuI	ACTGGG	1 cut(s)	2059
BpiI	GAAGAC	4 cut(s)	800, 1738, 1796, 2393
BplI	GAGNNNNNCTC	2 cut(s)	2460, 2492
BpmI	CTGGAG	1 cut(s)	1232
Bpu14I	TTCGAA	1 cut(s)	81
BpuEI	CTTGAG	6 cut(s)	37, 220, 837, 1893, 2462, 2568
BpuMI	CCSGG	4 cut(s)	70, 2002, 2066, 2744
Bsa29I	ATCGAT	2 cut(s)	216, 1867
BsaAI	YACGTR	1 cut(s)	2372
BsaHI	GRCGYC	1 cut(s)	587
BsaJI	CCNNGG	3 cut(s)	288, 1850, 2250
BsaWI	WCCGGW	1 cut(s)	944
Bsc4I	CCNNNNNNNGG	6 cut(s)	68, 167, 364, 889, 1851, 2458
Bse1I	ACTGG	7 cut(s)	910, 951, 1830, 1999, 2026, 2054, 2395
Bse3DI	GCAATG	1 cut(s)	557
BseAI	TCCGGA	1 cut(s)	944
BseBI	CCWGG	5 cut(s)	258, 827, 1250, 1773, 1851
BseCI	ATCGAT	2 cut(s)	216, 1867
BseDI	CCNNGG	3 cut(s)	288, 1850, 2250
BseGI	GGATG	6 cut(s)	11, 328, 941, 1048, 2752, 2809
BseLI	CCNNNNNNNGG	6 cut(s)	68, 167, 364, 889, 1851, 2458
BseMI	GCAATG	1 cut(s)	557
BseMII	CTCAG	4 cut(s)	528, 2277, 2448, 2682
BseNI	ACTGG	7 cut(s)	910, 951, 1830, 1999, 2026, 2054, 2395
BseRI	GAGGAG	1 cut(s)	1243
BseYI	CCCAGC	1 cut(s)	792
BsgI	GTGCAG	1 cut(s)	2654
Bsh1236I	CGCG	5 cut(s)	555, 659, 1616, 1664, 2432
BshFI	GGCC	3 cut(s)	2069, 2742, 2838
BshVI	ATCGAT	2 cut(s)	216, 1867
BsiHKAI	GWGCWC	3 cut(s)	1248, 1526, 2864
BsiHKCI	CYCGRG	1 cut(s)	1355
BsiSI	CCGG	6 cut(s)	69, 945, 2001, 2065, 2703, 2743
BslFI	GGGAC	1 cut(s)	2869
BslI	CCNNNNNNNGG	6 cut(s)	68, 167, 364, 889, 1851, 2458
BsmAI	GTCTC	4 cut(s)	101, 578, 1217, 2781
BsmBI	CGTCTC	3 cut(s)	578, 1217, 2781
BsmFI	GGGAC	1 cut(s)	2869
BsnI	GGCC	3 cut(s)	2069, 2742, 2838
BsoBI	CYCGRG	1 cut(s)	1355
Bsp119I	TTCGAA	1 cut(s)	81
Bsp1286I	GDGCHC	4 cut(s)	1248, 1526, 2552, 2864
Bsp13I	TCCGGA	1 cut(s)	944
Bsp143I	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
BspANI	GGCC	3 cut(s)	2069, 2742, 2838
BspCNI	CTCAG	4 cut(s)	529, 2276, 2449, 2683
BspDI	ATCGAT	2 cut(s)	216, 1867
BspEI	TCCGGA	1 cut(s)	944
BspFNI	CGCG	5 cut(s)	555, 659, 1616, 1664, 2432
BspHI	TCATGA	1 cut(s)	972
BspLI	GGNNCC	6 cut(s)	43, 282, 949, 1999, 2110, 2395
BspMI	ACCTGC	1 cut(s)	1143
BspOI	GCTAGC	1 cut(s)	2241
BspPI	GGATC	5 cut(s)	669, 1077, 1539, 1992, 2005
BspT104I	TTCGAA	1 cut(s)	81
BsrBI	CCGCTC	3 cut(s)	838, 1178, 1842
BsrDI	GCAATG	1 cut(s)	557
BsrI	ACTGG	7 cut(s)	910, 951, 1830, 1999, 2026, 2054, 2395
BssECI	CCNNGG	3 cut(s)	288, 1850, 2250
BssMI	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
BssNAI	GTATAC	1 cut(s)	1921
BssNI	GRCGYC	1 cut(s)	587
BssSI	CACGAG	2 cut(s)	626, 894
Bst1107I	GTATAC	1 cut(s)	1921
Bst2BI	CACGAG	2 cut(s)	626, 894
Bst2UI	CCWGG	5 cut(s)	258, 827, 1250, 1773, 1851
Bst4CI	ACNGT	7 cut(s)	226, 704, 1938, 2160, 2362, 2453, 2843
Bst6I	CTCTTC	3 cut(s)	894, 1182, 1290
BstACI	GRCGYC	1 cut(s)	587
BstAPI	GCANNNNNTGC	1 cut(s)	1169
BstBAI	YACGTR	1 cut(s)	2372
BstBI	TTCGAA	1 cut(s)	81
BstC8I	GCNNGC	7 cut(s)	119, 126, 1145, 2239, 2243, 2576, 2613
BstDEI	CTNAG	8 cut(s)	133, 251, 537, 1633, 2263, 2457, 2691, 2796
BstDSI	CCRYGG	1 cut(s)	2250
BstEII	GGTNACC	1 cut(s)	2408
BstENI	CCTNNNNNAGG	3 cut(s)	165, 362, 887
BstF5I	GGATG	6 cut(s)	11, 328, 941, 1048, 2752, 2809
BstFNI	CGCG	5 cut(s)	555, 659, 1616, 1664, 2432
BstHHI	GCGC	2 cut(s)	328, 557
BstKTI	GATC	9 cut(s)	206, 216, 664, 1050, 1072, 1534, 1949, 2000, 2428
BstMAI	GTCTC	4 cut(s)	101, 578, 1217, 2781
BstMBI	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
BstMWI	GCNNNNNNNGC	8 cut(s)	323, 334, 1144, 1169, 1688, 1910, 2312, 2696
BstNI	CCWGG	5 cut(s)	258, 827, 1250, 1773, 1851
BstNSI	RCATGY	3 cut(s)	523, 2245, 2369
BstPI	GGTNACC	1 cut(s)	2408
BstSCI	CCNGG	9 cut(s)	68, 256, 825, 1248, 1771, 1849, 2000, 2064, 2742
BstSFI	CTRYAG	2 cut(s)	19, 510
BstUI	CGCG	5 cut(s)	555, 659, 1616, 1664, 2432
BstV2I	GAAGAC	4 cut(s)	800, 1738, 1796, 2393
BstX2I	RGATCY	4 cut(s)	661, 1531, 1997, 2425
BstYI	RGATCY	4 cut(s)	661, 1531, 1997, 2425
BstZ17I	GTATAC	1 cut(s)	1921
Bsu15I	ATCGAT	2 cut(s)	216, 1867
BsuI	GTATCC	1 cut(s)	1955
BsuRI	GGCC	3 cut(s)	2069, 2742, 2838
BsuTUI	ATCGAT	2 cut(s)	216, 1867
BtgI	CCRYGG	1 cut(s)	2250
BtgZI	GCGATG	1 cut(s)	536
BtsCI	GGATG	6 cut(s)	11, 328, 941, 1048, 2752, 2809
BtsI	GCAGTG	1 cut(s)	2316
BtsIMutI	CAGTG	3 cut(s)	958, 2316, 2848
BveI	ACCTGC	1 cut(s)	1143
Cac8I	GCNNGC	7 cut(s)	119, 126, 1145, 2239, 2243, 2576, 2613
CciI	TCATGA	1 cut(s)	972
CfoI	GCGC	2 cut(s)	328, 557
Cfr13I	GGNCC	4 cut(s)	1497, 2024, 2067, 2740
ClaI	ATCGAT	2 cut(s)	216, 1867
CseI	GACGC	5 cut(s)	1212, 1653, 1670, 2657, 2763
CsiI	ACCWGGT	1 cut(s)	256
Csp6I	GTAC	6 cut(s)	222, 926, 2363, 2369, 2558, 2676
CviAII	CATG	9 cut(s)	430, 520, 601, 608, 973, 1679, 2242, 2366, 2555
CviQI	GTAC	6 cut(s)	222, 926, 2363, 2369, 2558, 2676
DdeI	CTNAG	8 cut(s)	133, 251, 537, 1633, 2263, 2457, 2691, 2796
DpnI	GATC	9 cut(s)	205, 215, 663, 1049, 1071, 1533, 1948, 1999, 2427
DpnII	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
DraI	TTTAAA	1 cut(s)	2325
DraIII	CACNNNGTG	2 cut(s)	631, 2251
EaeI	YGGCCR	1 cut(s)	2836
Eam1104I	CTCTTC	3 cut(s)	894, 1182, 1290
EarI	CTCTTC	3 cut(s)	894, 1182, 1290
Ecl136II	GAGCTC	3 cut(s)	1246, 1524, 2862
Eco24I	GRGCYC	4 cut(s)	1248, 1526, 2552, 2864
Eco47I	GGWCC	2 cut(s)	1497, 2024
Eco53kI	GAGCTC	3 cut(s)	1246, 1524, 2862
Eco57I	CTGAAG	4 cut(s)	132, 968, 1554, 1935
Eco88I	CYCGRG	1 cut(s)	1355
Eco91I	GGTNACC	1 cut(s)	2408
EcoICRI	GAGCTC	3 cut(s)	1246, 1524, 2862
EcoNI	CCTNNNNNAGG	3 cut(s)	165, 362, 887
EcoO65I	GGTNACC	1 cut(s)	2408
EcoRI	GAATTC	1 cut(s)	1471
EcoRII	CCWGG	5 cut(s)	256, 825, 1248, 1771, 1849
EcoT22I	ATGCAT	1 cut(s)	606
EcoT38I	GRGCYC	4 cut(s)	1248, 1526, 2552, 2864
Esp3I	CGTCTC	3 cut(s)	578, 1217, 2781
FaeI	CATG	9 cut(s)	433, 523, 604, 611, 976, 1682, 2245, 2369, 2558
FalI	AAGNNNNNCTT	2 cut(s)	1552, 1584
FaqI	GGGAC	1 cut(s)	2869
FatI	CATG	9 cut(s)	429, 519, 600, 607, 972, 1678, 2241, 2365, 2554
FauI	CCCGC	1 cut(s)	2425
FauNDI	CATATG	2 cut(s)	1129, 2119
FbaI	TGATCA	1 cut(s)	1047
FblI	GTMKAC	2 cut(s)	1920, 2448
FokI	GGATG	5 cut(s)	335, 928, 1055, 2759, 2816
FriOI	GRGCYC	4 cut(s)	1248, 1526, 2552, 2864
FspBI	CTAG	8 cut(s)	167, 329, 359, 453, 1173, 1400, 2238, 2901
GlaI	GCGC	2 cut(s)	327, 556
GsaI	CCCAGC	1 cut(s)	796
GsuI	CTGGAG	1 cut(s)	1232
HaeIII	GGCC	3 cut(s)	2069, 2742, 2838
HapII	CCGG	6 cut(s)	69, 945, 2001, 2065, 2703, 2743
HgaI	GACGC	5 cut(s)	1212, 1653, 1670, 2657, 2763
HhaI	GCGC	2 cut(s)	328, 557
Hin1I	GRCGYC	1 cut(s)	587
Hin1II	CATG	9 cut(s)	433, 523, 604, 611, 976, 1682, 2245, 2369, 2558
Hin6I	GCGC	2 cut(s)	326, 555
HinP1I	GCGC	2 cut(s)	326, 555
HincII	GTYRAC	1 cut(s)	2059
HindII	GTYRAC	1 cut(s)	2059
HindIII	AAGCTT	2 cut(s)	248, 1643
HpaII	CCGG	6 cut(s)	69, 945, 2001, 2065, 2703, 2743
HphI	GGTGA	5 cut(s)	582, 794, 1249, 1630, 1823
Hpy166II	GTNNAC	7 cut(s)	173, 1516, 1547, 1621, 1921, 2059, 2449
Hpy188III	TCNNGA	9 cut(s)	579, 896, 915, 945, 973, 1092, 1721, 2645, 2901
Hpy8I	GTNNAC	7 cut(s)	173, 1516, 1547, 1621, 1921, 2059, 2449
Hpy99I	CGWCG	2 cut(s)	463, 1228
HpyCH4III	ACNGT	7 cut(s)	226, 704, 1938, 2160, 2362, 2453, 2843
HpyCH4IV	ACGT	4 cut(s)	587, 1210, 1318, 2371
HpyF10VI	GCNNNNNNNGC	8 cut(s)	323, 334, 1144, 1169, 1688, 1910, 2312, 2696
HpyF3I	CTNAG	8 cut(s)	133, 251, 537, 1633, 2263, 2457, 2691, 2796
HpySE526I	ACGT	4 cut(s)	587, 1210, 1318, 2371
Hsp92I	GRCGYC	1 cut(s)	587
Hsp92II	CATG	9 cut(s)	433, 523, 604, 611, 976, 1682, 2245, 2369, 2558
HspAI	GCGC	2 cut(s)	326, 555
Kpn2I	TCCGGA	1 cut(s)	944
Ksp22I	TGATCA	1 cut(s)	1047
Kzo9I	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
LmnI	GCTCC	7 cut(s)	721, 859, 1180, 1243, 1251, 1847, 2114
LweI	GCATC	6 cut(s)	20, 313, 613, 950, 1891, 2224
MabI	ACCWGGT	1 cut(s)	256
MaeI	CTAG	8 cut(s)	167, 329, 359, 453, 1173, 1400, 2238, 2901
MaeII	ACGT	4 cut(s)	587, 1210, 1318, 2371
MaeIII	GTNAC	6 cut(s)	382, 588, 726, 2356, 2408, 2461
MalI	GATC	9 cut(s)	205, 215, 663, 1049, 1071, 1533, 1948, 1999, 2427
MbiI	CCGCTC	3 cut(s)	838, 1178, 1842
MboI	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
MflI	RGATCY	4 cut(s)	661, 1531, 1997, 2425
MhlI	GDGCHC	4 cut(s)	1248, 1526, 2552, 2864
MlsI	TGGCCA	1 cut(s)	2838
MluI	ACGCGT	1 cut(s)	1662
MluNI	TGGCCA	1 cut(s)	2838
MlyI	GAGTC	6 cut(s)	548, 1271, 1379, 1487, 1752, 2139
MmeI	TCCRAC	8 cut(s)	39, 215, 378, 482, 592, 743, 1089, 1475
Mox20I	TGGCCA	1 cut(s)	2838
Mph1103I	ATGCAT	1 cut(s)	606
MroI	TCCGGA	1 cut(s)	944
MroXI	GAANNNNTTC	1 cut(s)	77
MscI	TGGCCA	1 cut(s)	2838
MslI	CAYNNNNRTG	2 cut(s)	1809, 2370
Msp20I	TGGCCA	1 cut(s)	2838
MspA1I	CMGCKG	1 cut(s)	182
MspI	CCGG	6 cut(s)	69, 945, 2001, 2065, 2703, 2743
MspR9I	CCNGG	9 cut(s)	70, 258, 827, 1250, 1773, 1851, 2002, 2066, 2744
MvaI	CCWGG	5 cut(s)	258, 827, 1250, 1773, 1851
MvnI	CGCG	5 cut(s)	555, 659, 1616, 1664, 2432
MwoI	GCNNNNNNNGC	8 cut(s)	323, 334, 1144, 1169, 1688, 1910, 2312, 2696
NciI	CCSGG	4 cut(s)	70, 2002, 2066, 2744
NdeI	CATATG	2 cut(s)	1129, 2119
NdeII	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
NheI	GCTAGC	1 cut(s)	2237
NlaIII	CATG	9 cut(s)	433, 523, 604, 611, 976, 1682, 2245, 2369, 2558
NlaIV	GGNNCC	6 cut(s)	43, 282, 949, 1999, 2110, 2395
NmuCI	GTSAC	3 cut(s)	382, 588, 726
NsiI	ATGCAT	1 cut(s)	606
NspI	RCATGY	3 cut(s)	523, 2245, 2369
NspV	TTCGAA	1 cut(s)	81
PaeI	GCATGC	1 cut(s)	2245
PaeR7I	CTCGAG	1 cut(s)	1355
PagI	TCATGA	1 cut(s)	972
PciI	ACATGT	1 cut(s)	2365
PcsI	WCGNNNNNNNCGW	1 cut(s)	559
PdmI	GAANNNNTTC	1 cut(s)	77
PfeI	GAWTC	8 cut(s)	5, 750, 918, 1331, 1439, 1574, 2255, 2876
PflFI	GACNNNGTC	1 cut(s)	258
PfoI	TCCNGGA	3 cut(s)	825, 1248, 2000
PleI	GAGTC	6 cut(s)	547, 1270, 1378, 1486, 1751, 2138
PpsI	GAGTC	6 cut(s)	547, 1270, 1378, 1486, 1751, 2138
Ppu21I	YACGTR	1 cut(s)	2372
PscI	ACATGT	1 cut(s)	2365
PshBI	ATTAAT	2 cut(s)	1055, 2759
Psp124BI	GAGCTC	3 cut(s)	1248, 1526, 2864
Psp1406I	AACGTT	1 cut(s)	1318
Psp6I	CCWGG	5 cut(s)	256, 825, 1248, 1771, 1849
PspEI	GGTNACC	1 cut(s)	2408
PspFI	CCCAGC	1 cut(s)	792
PspGI	CCWGG	5 cut(s)	256, 825, 1248, 1771, 1849
PspN4I	GGNNCC	6 cut(s)	43, 282, 949, 1999, 2110, 2395
PspPI	GGNCC	4 cut(s)	1497, 2024, 2067, 2740
PspXI	VCTCGAGB	1 cut(s)	1355
PsuI	RGATCY	4 cut(s)	661, 1531, 1997, 2425
PsyI	GACNNNGTC	1 cut(s)	258
PvuII	CAGCTG	1 cut(s)	182
RsaI	GTAC	6 cut(s)	223, 927, 2364, 2370, 2559, 2677
RsaNI	GTAC	6 cut(s)	222, 926, 2363, 2369, 2558, 2676
RseI	CAYNNNNRTG	2 cut(s)	1809, 2370
SacI	GAGCTC	3 cut(s)	1248, 1526, 2864
Sau3AI	GATC	9 cut(s)	203, 213, 661, 1047, 1069, 1531, 1946, 1997, 2425
Sau96I	GGNCC	4 cut(s)	1497, 2024, 2067, 2740
ScaI	AGTACT	2 cut(s)	927, 2677
SchI	GAGTC	6 cut(s)	548, 1271, 1379, 1487, 1752, 2139
ScrFI	CCNGG	9 cut(s)	70, 258, 827, 1250, 1773, 1851, 2002, 2066, 2744
SduI	GDGCHC	4 cut(s)	1248, 1526, 2552, 2864
SexAI	ACCWGGT	1 cut(s)	256
SfaNI	GCATC	6 cut(s)	20, 313, 613, 950, 1891, 2224
SfcI	CTRYAG	2 cut(s)	19, 510
Sfr274I	CTCGAG	1 cut(s)	1355
SfuI	TTCGAA	1 cut(s)	81
SinI	GGWCC	2 cut(s)	1497, 2024
SlaI	CTCGAG	1 cut(s)	1355
SmiMI	CAYNNNNRTG	2 cut(s)	1809, 2370
SmlI	CTYRAG	7 cut(s)	52, 199, 852, 1355, 1908, 2477, 2583
SmoI	CTYRAG	7 cut(s)	52, 199, 852, 1355, 1908, 2477, 2583
SpeI	ACTAGT	1 cut(s)	452
SphI	GCATGC	1 cut(s)	2245
SspMI	CTAG	8 cut(s)	167, 329, 359, 453, 1173, 1400, 2238, 2901
SstI	GAGCTC	3 cut(s)	1248, 1526, 2864
StyD4I	CCNGG	9 cut(s)	68, 256, 825, 1248, 1771, 1849, 2000, 2064, 2742
TaaI	ACNGT	7 cut(s)	226, 704, 1938, 2160, 2362, 2453, 2843
TaiI	ACGT	4 cut(s)	590, 1213, 1321, 2374
TaqI	TCGA	8 cut(s)	81, 216, 756, 1226, 1356, 1481, 1753, 1867
TatI	WGTACW	3 cut(s)	925, 2362, 2675
TauI	GCSGC	2 cut(s)	124, 841
TfiI	GAWTC	8 cut(s)	5, 750, 918, 1331, 1439, 1574, 2255, 2876
TscAI	CASTG	3 cut(s)	958, 2323, 2848
TseFI	GTSAC	3 cut(s)	382, 588, 726
Tsp45I	GTSAC	3 cut(s)	382, 588, 726
TspGWI	ACGGA	1 cut(s)	1172
TspRI	CASTG	3 cut(s)	958, 2323, 2848
Tth111I	GACNNNGTC	1 cut(s)	258
VpaK11BI	GGWCC	2 cut(s)	1497, 2024
VspI	ATTAAT	2 cut(s)	1055, 2759
XagI	CCTNNNNNAGG	3 cut(s)	165, 362, 887
XapI	RAATTY	6 cut(s)	545, 678, 1086, 1255, 1471, 1716
XbaI	TCTAGA	1 cut(s)	2900
XceI	RCATGY	3 cut(s)	523, 2245, 2369
XhoI	CTCGAG	1 cut(s)	1355
XmiI	GTMKAC	2 cut(s)	1920, 2448
XmnI	GAANNNNTTC	1 cut(s)	77
XspI	CTAG	8 cut(s)	167, 329, 359, 453, 1173, 1400, 2238, 2901
ZraI	GACGTC	1 cut(s)	588
ZrmI	AGTACT	2 cut(s)	927, 2677
Zsp2I	ATGCAT	1 cut(s)	606

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.