Rorug06G0469200
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0004278
Physical Location & Seq
Reverse (-)
59315778 .. 59318079
2302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0469200.1

Sequence Viewer

Length: 807 bp
ATGGAGAAAGCAAGTGCATTCCTTTGCCATCCCCCTCCCTCACGTGGAAAGCTCATCACTGTTCTCAGCATTGATGGAGGTGGAATCAAAGGGATTATCCCCGGGACTCTGCTCAGTTTCCTAGAGTCCGAGCTCCAGAAGCTGGATGGTGAAGCTGCAAGGTTGGCAGATTATTTCGATGTTATTGCAGGGACTAGCACAGGTGGCCTTGTCACCGCCATGCTTACCGCCCCGGATCAAAACAACCGTCCCTTGTTTGCGGCTAACCAGATTGTTGATTTTTACCTTGAGCACTGCCCTAAAATATTCCCTCAAATCAATATTCCACTGTTTGGTGACGCCTTAGAAAATGCAAAGGGTGTATACGCACCAAAATATGATGGCAAGTATTTACACAAGCTTATCAAGGAAAAATTAGGAGACAGACGATTGAACCAGACATTGACCAATGTTGTGATCCCAACTTTTGATATAAGTTGCCTCCAACCAGCTATCTTCTCCACCTACGAGTTGAAAAGGAACAAGAAATTAGATCCGGCTCTACTGTCGGATATATGCATCGGAACTTCAGCAGCACCAACTTATCTTCCCTCGCATTATTTCAAAACCGCAACAAGAGAATTCCATCTCGGTTTGACTGCTGTAACCGAAGTGACAAAAGAAATCAAGAAGGGAAATACTGACTTCTCTCCGATTGAGGCAGCTGATCAGGGTAATAGGTTCCTCCTCATATCTTTGGGTACTGGTTCATCAGCAAAATCCAAGTACGATGCCAGAGTAGCAGCTAAGTGGGGTATTATGGCATAG

Protein Analysis

268

Amino Acids

29.17

Weight (kDa)

8.54

Isoelectric Point (pI)

33.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 22 - 201 1.8e-16 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 142, 332
AccI GTMKAC 1 cut(s) 363
AciI CCGC 4 cut(s) 216, 228, 260, 609
AclWI GGATC 3 cut(s) 243, 451, 527
AcsI RAATTY 1 cut(s) 620
AcuI CTGAAG 1 cut(s) 552
AcvI CACGTG 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 339
AfaI GTAC 2 cut(s) 742, 767
AfiI CCNNNNNNNGG 3 cut(s) 44, 142, 332
AgsI TTSAA 3 cut(s) 433, 514, 604
AluBI AGCT 8 cut(s) 52, 133, 142, 155, 400, 491, 704, 785
AluI AGCT 8 cut(s) 52, 133, 142, 155, 400, 491, 704, 785
Alw21I GWGCWC 2 cut(s) 135, 294
Alw26I GTCTC 1 cut(s) 414
AlwI GGATC 3 cut(s) 243, 451, 527
AlwNI CAGNNNCTG 1 cut(s) 142
Ama87I CYCGRG 1 cut(s) 101
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 4 cut(s) 155, 572, 701, 782
ApoI RAATTY 1 cut(s) 620
ArsI GACNNNNNNTTYG 2 cut(s) 232, 264
AsuC2I CCSGG 3 cut(s) 102, 103, 233
AsuHPI GGTGA 3 cut(s) 161, 205, 347
AvaI CYCGRG 1 cut(s) 101
BanII GRGCYC 1 cut(s) 135
BbrPI CACGTG 1 cut(s) 44
Bbv12I GWGCWC 2 cut(s) 135, 294
BbvI GCAGC 4 cut(s) 142, 584, 713, 794
BccI CCATC 5 cut(s) 36, 68, 140, 374, 633
BcgI CGANNNNNNTGC 2 cut(s) 167, 201
BclI TGATCA 1 cut(s) 706
BcnI CCSGG 3 cut(s) 102, 103, 233
BcoDI GTCTC 1 cut(s) 414
BfaI CTAG 2 cut(s) 122, 195
BisI GCNGC 5 cut(s) 156, 261, 573, 702, 783
BlsI GCNGC 5 cut(s) 157, 262, 574, 703, 784
Bme1390I CCNGG 3 cut(s) 102, 103, 233
BmeT110I CYCGRG 1 cut(s) 101
BmiI GGNNCC 1 cut(s) 722
BmrFI CCNGG 3 cut(s) 102, 103, 233
BmsI GCATC 2 cut(s) 567, 760
BpmI CTGGAG 1 cut(s) 119
BpuEI CTTGAG 1 cut(s) 308
BpuMI CCSGG 3 cut(s) 102, 103, 233
BsaAI YACGTR 1 cut(s) 44
BsaHI GRCGYC 1 cut(s) 339
BsaJI CCNNGG 3 cut(s) 100, 101, 231
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 142, 332
Bse1I ACTGG 1 cut(s) 748
BseDI CCNNGG 3 cut(s) 100, 101, 231
BseGI GGATG 2 cut(s) 28, 151
BseLI CCNNNNNNNGG 3 cut(s) 44, 142, 332
BseMII CTCAG 2 cut(s) 79, 127
BseNI ACTGG 1 cut(s) 748
BseRI GAGGAG 1 cut(s) 716
BseXI GCAGC 4 cut(s) 142, 584, 713, 794
BshFI GGCC 1 cut(s) 207
BsiHKAI GWGCWC 2 cut(s) 135, 294
BsiHKCI CYCGRG 1 cut(s) 101
BsiSI CCGG 3 cut(s) 102, 233, 536
BslFI GGGAC 3 cut(s) 118, 205, 234
BslI CCNNNNNNNGG 3 cut(s) 44, 142, 332
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 3 cut(s) 118, 205, 234
BsmI GAATGC 1 cut(s) 17
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 101
Bsp1286I GDGCHC 2 cut(s) 135, 294
Bsp143I GATC 4 cut(s) 235, 456, 532, 706
BspACI CCGC 4 cut(s) 216, 228, 260, 609
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 2 cut(s) 78, 126
BspLI GGNNCC 1 cut(s) 722
BspPI GGATC 3 cut(s) 243, 451, 527
BsrI ACTGG 1 cut(s) 748
BssECI CCNNGG 3 cut(s) 100, 101, 231
BssMI GATC 4 cut(s) 235, 456, 532, 706
BssNAI GTATAC 1 cut(s) 364
BssNI GRCGYC 1 cut(s) 339
Bst1107I GTATAC 1 cut(s) 364
Bst4CI ACNGT 4 cut(s) 61, 248, 330, 546
BstACI GRCGYC 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 44
BstDEI CTNAG 4 cut(s) 65, 113, 343, 786
BstF5I GGATG 2 cut(s) 28, 151
BstKTI GATC 4 cut(s) 238, 459, 535, 709
BstMAI GTCTC 1 cut(s) 414
BstMBI GATC 4 cut(s) 235, 456, 532, 706
BstMWI GCNNNNNNNGC 4 cut(s) 139, 164, 204, 779
BstSCI CCNGG 3 cut(s) 100, 101, 231
BstV1I GCAGC 4 cut(s) 142, 584, 713, 794
BstX2I RGATCY 1 cut(s) 532
BstYI RGATCY 1 cut(s) 532
BstZ17I GTATAC 1 cut(s) 364
BsuRI GGCC 1 cut(s) 207
BtsCI GGATG 2 cut(s) 28, 151
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 3 cut(s) 57, 292, 326
CaiI CAGNNNCTG 1 cut(s) 142
Cfr9I CCCGGG 1 cut(s) 101
CseI GACGC 1 cut(s) 347
Csp6I GTAC 2 cut(s) 741, 766
CviAII CATG 1 cut(s) 220
CviQI GTAC 2 cut(s) 741, 766
DdeI CTNAG 4 cut(s) 65, 113, 343, 786
DpnI GATC 4 cut(s) 237, 458, 534, 708
DpnII GATC 4 cut(s) 235, 456, 532, 706
Ecl136II GAGCTC 1 cut(s) 133
Eco24I GRGCYC 1 cut(s) 135
Eco53kI GAGCTC 1 cut(s) 133
Eco57I CTGAAG 1 cut(s) 552
Eco72I CACGTG 1 cut(s) 44
Eco88I CYCGRG 1 cut(s) 101
EcoICRI GAGCTC 1 cut(s) 133
EcoRI GAATTC 1 cut(s) 620
EcoT22I ATGCAT 1 cut(s) 560
EcoT38I GRGCYC 1 cut(s) 135
FaeI CATG 1 cut(s) 223
FaiI YATR 9 cut(s) 221, 364, 378, 473, 554, 556, 731, 800, 805
FaqI GGGAC 3 cut(s) 118, 205, 234
FatI CATG 1 cut(s) 219
FbaI TGATCA 1 cut(s) 706
FblI GTMKAC 1 cut(s) 363
Fnu4HI GCNGC 5 cut(s) 156, 261, 573, 702, 783
FokI GGATG 2 cut(s) 15, 158
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 5 cut(s) 156, 261, 573, 702, 783
FspBI CTAG 2 cut(s) 122, 195
GluI GCNGC 5 cut(s) 156, 261, 573, 702, 783
GsuI CTGGAG 1 cut(s) 119
HaeIII GGCC 1 cut(s) 207
HapII CCGG 3 cut(s) 102, 233, 536
HgaI GACGC 1 cut(s) 347
Hin1I GRCGYC 1 cut(s) 339
Hin1II CATG 1 cut(s) 223
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 3 cut(s) 84, 106, 125
HpaII CCGG 3 cut(s) 102, 233, 536
HphI GGTGA 3 cut(s) 161, 205, 347
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 4 cut(s) 130, 550, 563, 693
Hpy188III TCNNGA 2 cut(s) 136, 667
Hpy8I GTNNAC 1 cut(s) 364
HpyAV CCTTC 1 cut(s) 664
HpyCH4III ACNGT 4 cut(s) 61, 248, 330, 546
HpyCH4IV ACGT 1 cut(s) 43
HpyCH4V TGCA 5 cut(s) 17, 158, 188, 353, 558
HpyF10VI GCNNNNNNNGC 4 cut(s) 139, 164, 204, 779
HpyF3I CTNAG 4 cut(s) 65, 113, 343, 786
HpySE526I ACGT 1 cut(s) 43
Hsp92I GRCGYC 1 cut(s) 339
Hsp92II CATG 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 706
Kzo9I GATC 4 cut(s) 235, 456, 532, 706
LmnI GCTCC 1 cut(s) 138
Lsp1109I GCAGC 4 cut(s) 142, 584, 713, 794
LweI GCATC 2 cut(s) 567, 760
MaeI CTAG 2 cut(s) 122, 195
MaeII ACGT 1 cut(s) 43
MaeIII GTNAC 4 cut(s) 211, 335, 643, 652
MalI GATC 4 cut(s) 237, 458, 534, 708
MboI GATC 4 cut(s) 235, 456, 532, 706
MboII GAAGA 2 cut(s) 487, 578
MflI RGATCY 1 cut(s) 532
MhlI GDGCHC 2 cut(s) 135, 294
MluCI AATT 3 cut(s) 413, 527, 620
MlyI GAGTC 2 cut(s) 100, 134
MmeI TCCRAC 2 cut(s) 508, 528
MnlI CCTC 9 cut(s) 45, 49, 71, 321, 491, 601, 691, 734, 737
Mph1103I ATGCAT 1 cut(s) 560
MslI CAYNNNNRTG 1 cut(s) 218
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 3 cut(s) 102, 233, 536
MspR9I CCNGG 3 cut(s) 102, 103, 233
Mva1269I GAATGC 1 cut(s) 17
MwoI GCNNNNNNNGC 4 cut(s) 139, 164, 204, 779
NciI CCSGG 3 cut(s) 102, 103, 233
NdeII GATC 4 cut(s) 235, 456, 532, 706
NlaIII CATG 1 cut(s) 223
NlaIV GGNNCC 1 cut(s) 722
NmuCI GTSAC 3 cut(s) 211, 335, 652
NsiI ATGCAT 1 cut(s) 560
PctI GAATGC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 84
PflMI CCANNNNNTGG 2 cut(s) 142, 332
PkrI GCNGC 5 cut(s) 157, 262, 574, 703, 784
PleI GAGTC 2 cut(s) 100, 133
PmaCI CACGTG 1 cut(s) 44
PmlI CACGTG 1 cut(s) 44
PpsI GAGTC 2 cut(s) 100, 133
Ppu21I YACGTR 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 135
PspCI CACGTG 1 cut(s) 44
PspN4I GGNNCC 1 cut(s) 722
PstNI CAGNNNCTG 1 cut(s) 142
PsuI RGATCY 1 cut(s) 532
PvuII CAGCTG 1 cut(s) 704
RsaI GTAC 2 cut(s) 742, 767
RsaNI GTAC 2 cut(s) 741, 766
RseI CAYNNNNRTG 1 cut(s) 218
SacI GAGCTC 1 cut(s) 135
SatI GCNGC 5 cut(s) 156, 261, 573, 702, 783
Sau3AI GATC 4 cut(s) 235, 456, 532, 706
SchI GAGTC 2 cut(s) 100, 134
ScrFI CCNGG 3 cut(s) 102, 103, 233
SduI GDGCHC 2 cut(s) 135, 294
SfaNI GCATC 2 cut(s) 567, 760
SmaI CCCGGG 1 cut(s) 103
SmiMI CAYNNNNRTG 1 cut(s) 218
SmlI CTYRAG 1 cut(s) 287
SmoI CTYRAG 1 cut(s) 287
Sse9I AATT 3 cut(s) 413, 527, 620
SsiI CCGC 4 cut(s) 216, 228, 260, 609
SspI AATATT 2 cut(s) 306, 322
SspMI CTAG 2 cut(s) 122, 195
SstI GAGCTC 1 cut(s) 135
StyD4I CCNGG 3 cut(s) 100, 101, 231
TaaI ACNGT 4 cut(s) 61, 248, 330, 546
TaiI ACGT 1 cut(s) 46
TaqI TCGA 1 cut(s) 177
TasI AATT 3 cut(s) 413, 527, 620
TauI GCSGC 1 cut(s) 263
TfiI GAWTC 1 cut(s) 84
TscAI CASTG 3 cut(s) 64, 299, 333
TseFI GTSAC 3 cut(s) 211, 335, 652
TseI GCWGC 4 cut(s) 155, 572, 701, 782
Tsp45I GTSAC 3 cut(s) 211, 335, 652
TspDTI ATGAA 1 cut(s) 738
TspMI CCCGGG 1 cut(s) 101
TspRI CASTG 3 cut(s) 64, 299, 333
Van91I CCANNNNNTGG 2 cut(s) 142, 332
XapI RAATTY 1 cut(s) 620
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XmaI CCCGGG 1 cut(s) 101
XmiI GTMKAC 1 cut(s) 363
XspI CTAG 2 cut(s) 122, 195
Zsp2I ATGCAT 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.