Rroxscaffold_3G00268930
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004278
Physical Location & Seq
Reverse (-)
61929678 .. 61931623
1946 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00268930.1

Sequence Viewer

Length: 1053 bp
ATGCACCCTGTCAAATCAACCTCAGATGTCATTAAATTAATGGATATCGGATTTAAGAACCGAGCTGTTAGTGCCACTGCCCTCAACGAAAGAAGTAGTCGCTCTCATAGTGTTCTTACAGTTCATGTACGAGGGACGGATCTGAAGACTAGTTCTGCTTTGGTCGGCAATCTTCATCTTGTAGATTTGGCAGGAAGTGAAAGGGTAGATCGCTCTGAGGTTACTGGAGATAGACTTAAGGAAGCACAACATATAAACAAATCATTATCTTCACTTGGAGATGTCATATTTGCTCTTGCACAGAAGAGCGCTCATGTACCATACAGAAACAGCAAGCTTACTCAAGTCCTTCAAAGCTCTCTCGGTGGACAAGCAAAAACACTTATGTTTGTGCAGCTTAATCCTGAAACAAGTTCATATTCTGAAAGTTTAAGTACTCTAAAGTTTGCTGAGAGGGTTGCTGGAGTAGAGCTAGGGGCGGCAAAAAGCAACAAAGAGGGCAGGGATGCTAGGGAGTTGATGGAACAGGTGGCAATGCTCAAAGACACTATATCGAAGAAGGATAATGAGATAGAACGACTGCATTTACTTAAAGATATCCGAGTGGAGAAGCCTCGGACAGGCTCGTTAAGTTCTGAAACTGATTCCCAGCAGTCCATGCATGACCTTAAACACCAAAATAATACGCTCCGGCGATCAAAGGTTGCCGGAGGTGACATAGGTCAGAATAATTCTGCGGATGCTGAGATGTTAGGATTTGGAGAGGCAGATTATGAGGAGAAATTAAGTGACTTATCTGACGGTGGTCTTTCTGGTGGAACAGAAACTGATGGCTCCGCCTCTGCTGACAGTACGAGCTATTCTGAAAGCACAAAATCAGATAGCTTAGAAAAGCCTGGAAGGGTTTCTAGGATTCCCCGACCTTGGTCAAAACCAGCAGTAACTTCCCCATCTATAATGTCAGGATCAAAAAGCTCTCCGAAAGTTTCATCAGGTCTAAAAAAAAGTAGCAGCTCTTCCTCACTGAAACCAGCTTCTAAACGATGGCAATAA

Protein Analysis

350

Amino Acids

37.69

Weight (kDa)

9.25

Isoelectric Point (pI)

44.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 2 - 154 2.6e-59 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 479, 737, 837
AclWI GGATC 2 cut(s) 147, 973
AcuI CTGAAG 1 cut(s) 164
AfaI GTAC 4 cut(s) 129, 318, 436, 853
AfeI AGCGCT 1 cut(s) 310
AfiI CCNNNNNNNGG 2 cut(s) 620, 924
AflII CTTAAG 1 cut(s) 236
AgsI TTSAA 1 cut(s) 353
AhlI ACTAGT 1 cut(s) 149
AjnI CCWGG 1 cut(s) 895
AlwI GGATC 2 cut(s) 147, 973
AlwNI CAGNNNCTG 1 cut(s) 827
Aor51HI AGCGCT 1 cut(s) 310
ApeKI GCWGC 2 cut(s) 394, 1011
AseI ATTAAT 1 cut(s) 38
Asp700I GAANNNNTTC 1 cut(s) 904
AspLEI GCGC 1 cut(s) 311
AsuHPI GGTGA 1 cut(s) 725
BarI GAAGNNNNNNTAC 2 cut(s) 1000, 1032
BbsI GAAGAC 1 cut(s) 152
BbvI GCAGC 2 cut(s) 406, 1023
BccI CCATC 4 cut(s) 514, 824, 958, 1038
BciT130I CCWGG 1 cut(s) 897
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 4 cut(s) 150, 473, 510, 909
BfoI RGCGCY 1 cut(s) 312
BfrI CTTAAG 1 cut(s) 236
BisI GCNGC 3 cut(s) 395, 480, 1012
BlsI GCNGC 3 cut(s) 396, 481, 1013
BmcAI AGTACT 1 cut(s) 436
Bme1390I CCNGG 1 cut(s) 897
BmiI GGNNCC 1 cut(s) 835
BmrFI CCNGG 1 cut(s) 897
BmsI GCATC 2 cut(s) 496, 730
BoxI GACNNNNGTC 3 cut(s) 720, 804, 925
BpiI GAAGAC 1 cut(s) 152
BpmI CTGGAG 2 cut(s) 246, 483
BpuEI CTTGAG 1 cut(s) 327
BsaJI CCNNGG 2 cut(s) 614, 923
Bsc4I CCNNNNNNNGG 2 cut(s) 620, 924
Bse1I ACTGG 1 cut(s) 229
Bse3DI GCAATG 1 cut(s) 540
BseBI CCWGG 1 cut(s) 897
BseDI CCNNGG 2 cut(s) 614, 923
BseGI GGATG 2 cut(s) 511, 745
BseLI CCNNNNNNNGG 2 cut(s) 620, 924
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 4 cut(s) 36, 207, 441, 735
BseNI ACTGG 1 cut(s) 229
BseRI GAGGAG 1 cut(s) 791
BseXI GCAGC 2 cut(s) 406, 1023
BseYI CCCAGC 1 cut(s) 648
BsgI GTGCAG 1 cut(s) 413
BsiSI CCGG 2 cut(s) 691, 708
BslFI GGGAC 1 cut(s) 148
BslI CCNNNNNNNGG 2 cut(s) 620, 924
BsmFI GGGAC 1 cut(s) 148
Bsp143I GATC 4 cut(s) 139, 208, 695, 965
BspACI CCGC 3 cut(s) 479, 737, 837
BspCNI CTCAG 4 cut(s) 35, 208, 442, 736
BspLI GGNNCC 1 cut(s) 835
BspPI GGATC 2 cut(s) 147, 973
BspQI GCTCTTC 2 cut(s) 299, 1021
BspTI CTTAAG 1 cut(s) 236
BsrDI GCAATG 1 cut(s) 540
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 2 cut(s) 614, 923
BssMI GATC 4 cut(s) 139, 208, 695, 965
BssT1I CCWWGG 1 cut(s) 923
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 3 cut(s) 121, 803, 851
Bst6I CTCTTC 2 cut(s) 299, 1021
BstAFI CTTAAG 1 cut(s) 236
BstAPI GCANNNNNTGC 1 cut(s) 658
BstC8I GCNNGC 1 cut(s) 335
BstDEI CTNAG 5 cut(s) 22, 216, 450, 744, 886
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 2 cut(s) 511, 745
BstH2I RGCGCY 1 cut(s) 312
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 4 cut(s) 142, 211, 698, 968
BstMBI GATC 4 cut(s) 139, 208, 695, 965
BstMWI GCNNNNNNNGC 2 cut(s) 71, 658
BstNI CCWGG 1 cut(s) 897
BstPAI GACNNNNGTC 3 cut(s) 720, 804, 925
BstSCI CCNGG 1 cut(s) 895
BstV1I GCAGC 2 cut(s) 406, 1023
BstV2I GAAGAC 1 cut(s) 152
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BtsCI GGATG 2 cut(s) 511, 745
BtsI GCAGTG 1 cut(s) 75
BtsIMutI CAGTG 2 cut(s) 75, 1022
Cac8I GCNNGC 1 cut(s) 335
CaiI CAGNNNCTG 1 cut(s) 827
CfoI GCGC 1 cut(s) 311
Csp6I GTAC 4 cut(s) 128, 317, 435, 852
CviAII CATG 4 cut(s) 125, 314, 658, 662
CviQI GTAC 4 cut(s) 128, 317, 435, 852
DdeI CTNAG 5 cut(s) 22, 216, 450, 744, 886
DpnI GATC 4 cut(s) 141, 210, 697, 967
DpnII GATC 4 cut(s) 139, 208, 695, 965
Eam1104I CTCTTC 2 cut(s) 299, 1021
EarI CTCTTC 2 cut(s) 299, 1021
EciI GGCGGA 1 cut(s) 826
Eco130I CCWWGG 1 cut(s) 923
Eco32I GATATC 2 cut(s) 46, 598
Eco47III AGCGCT 1 cut(s) 310
Eco57I CTGAAG 1 cut(s) 164
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoRII CCWGG 1 cut(s) 895
EcoRV GATATC 2 cut(s) 46, 598
EcoT14I CCWWGG 1 cut(s) 923
EcoT22I ATGCAT 1 cut(s) 663
ErhI CCWWGG 1 cut(s) 923
FaeI CATG 4 cut(s) 128, 317, 661, 665
FaqI GGGAC 1 cut(s) 148
FatI CATG 4 cut(s) 124, 313, 657, 661
Fnu4HI GCNGC 3 cut(s) 395, 480, 1012
FokI GGATG 2 cut(s) 518, 752
Fsp4HI GCNGC 3 cut(s) 395, 480, 1012
FspBI CTAG 4 cut(s) 150, 473, 510, 909
GlaI GCGC 1 cut(s) 310
GluI GCNGC 3 cut(s) 395, 480, 1012
GsaI CCCAGC 1 cut(s) 652
GsuI CTGGAG 2 cut(s) 246, 483
HaeII RGCGCY 1 cut(s) 312
HapII CCGG 2 cut(s) 691, 708
HhaI GCGC 1 cut(s) 311
Hin1II CATG 4 cut(s) 128, 317, 661, 665
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HindIII AAGCTT 1 cut(s) 335
HinfI GANTC 2 cut(s) 644, 913
HpaII CCGG 2 cut(s) 691, 708
HphI GGTGA 1 cut(s) 725
Hpy166II GTNNAC 1 cut(s) 368
Hpy188III TCNNGA 2 cut(s) 404, 963
Hpy8I GTNNAC 1 cut(s) 368
HpyAV CCTTC 3 cut(s) 359, 553, 894
HpyCH4III ACNGT 3 cut(s) 121, 803, 851
HpyCH4V TGCA 5 cut(s) 4, 299, 394, 583, 661
HpyF10VI GCNNNNNNNGC 2 cut(s) 71, 658
HpyF3I CTNAG 5 cut(s) 22, 216, 450, 744, 886
Hsp92II CATG 4 cut(s) 128, 317, 661, 665
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 4 cut(s) 139, 208, 695, 965
LguI GCTCTTC 2 cut(s) 299, 1021
LmnI GCTCC 2 cut(s) 693, 839
Lsp1109I GCAGC 2 cut(s) 406, 1023
LweI GCATC 2 cut(s) 496, 730
MaeI CTAG 4 cut(s) 150, 473, 510, 909
MaeIII GTNAC 4 cut(s) 220, 713, 788, 940
MalI GATC 4 cut(s) 141, 210, 697, 967
MboI GATC 4 cut(s) 139, 208, 695, 965
MboII GAAGA 6 cut(s) 157, 164, 261, 316, 568, 1008
MflI RGATCY 1 cut(s) 139
MluCI AATT 3 cut(s) 35, 730, 782
Mph1103I ATGCAT 1 cut(s) 663
MroXI GAANNNNTTC 1 cut(s) 904
MspCI CTTAAG 1 cut(s) 236
MspI CCGG 2 cut(s) 691, 708
MspR9I CCNGG 1 cut(s) 897
MvaI CCWGG 1 cut(s) 897
MwoI GCNNNNNNNGC 2 cut(s) 71, 658
NdeII GATC 4 cut(s) 139, 208, 695, 965
NlaIII CATG 4 cut(s) 128, 317, 661, 665
NlaIV GGNNCC 1 cut(s) 835
NmuCI GTSAC 2 cut(s) 713, 788
NsiI ATGCAT 1 cut(s) 663
PciSI GCTCTTC 2 cut(s) 299, 1021
PdmI GAANNNNTTC 1 cut(s) 904
PfeI GAWTC 2 cut(s) 644, 913
PkrI GCNGC 3 cut(s) 396, 481, 1013
PshAI GACNNNNGTC 3 cut(s) 720, 804, 925
PshBI ATTAAT 1 cut(s) 38
Psp6I CCWGG 1 cut(s) 895
PspFI CCCAGC 1 cut(s) 648
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 1 cut(s) 835
PstNI CAGNNNCTG 1 cut(s) 827
PsuI RGATCY 1 cut(s) 139
RsaI GTAC 4 cut(s) 129, 318, 436, 853
RsaNI GTAC 4 cut(s) 128, 317, 435, 852
SapI GCTCTTC 2 cut(s) 299, 1021
SatI GCNGC 3 cut(s) 395, 480, 1012
Sau3AI GATC 4 cut(s) 139, 208, 695, 965
ScaI AGTACT 1 cut(s) 436
ScrFI CCNGG 1 cut(s) 897
SfaNI GCATC 2 cut(s) 496, 730
SmlI CTYRAG 2 cut(s) 236, 342
SmoI CTYRAG 2 cut(s) 236, 342
SpeI ACTAGT 1 cut(s) 149
Sse9I AATT 3 cut(s) 35, 730, 782
SsiI CCGC 3 cut(s) 479, 737, 837
SspMI CTAG 4 cut(s) 150, 473, 510, 909
StyD4I CCNGG 1 cut(s) 895
StyI CCWWGG 1 cut(s) 923
TaaI ACNGT 3 cut(s) 121, 803, 851
TaqI TCGA 1 cut(s) 554
TasI AATT 3 cut(s) 35, 730, 782
TatI WGTACW 1 cut(s) 434
TauI GCSGC 1 cut(s) 482
TfiI GAWTC 2 cut(s) 644, 913
TscAI CASTG 2 cut(s) 82, 1029
TseFI GTSAC 2 cut(s) 713, 788
TseI GCWGC 2 cut(s) 394, 1011
Tsp45I GTSAC 2 cut(s) 713, 788
TspDTI ATGAA 4 cut(s) 113, 164, 405, 978
TspGWI ACGGA 1 cut(s) 152
TspRI CASTG 2 cut(s) 82, 1029
Vha464I CTTAAG 1 cut(s) 236
VspI ATTAAT 1 cut(s) 38
XagI CCTNNNNNAGG 1 cut(s) 618
XmnI GAANNNNTTC 1 cut(s) 904
XspI CTAG 4 cut(s) 150, 473, 510, 909
ZrmI AGTACT 1 cut(s) 436
Zsp2I ATGCAT 1 cut(s) 663
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.