Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7D

Orthogroup ID

OG0004278

Physical Location & Seq

Genomic Coordinates Forward (+)

5751576 .. 5759308

Gene Structure 7733 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7DG074200.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2976 bp

ATGATGGATTCCTCTTCTGATAACCACAAAACTCAAAAGGGAGGAACCTCGACCTCAAGTGTTCCTGCTAATGGGGATATGTTTGGACGAGTTGTAAATGGTACTGGTGAGACTGAAGGCAAGCGGCTAGCGTTTTTAGTGGAGTGGTTAAATAGTATACTTCCTGATTTAGGTTTACCAGAAACTGCTTCAGATGAGGACTTGAGATCAGTATTGATTGATGGTACCATTTTATGTCGGCTATTGAACAGGCTTAGACCTTGTTCTGTTGATGAGGTTAATAGATCCGATCAGAACTCAGTGCCAAGCTCAGAAAATGTCGCAAGGTTCTTGGCTGCTGTTGATTCATTAGGCCTGCCCCAGTTTAATATGGCGGATCTAGAGAAGGGATCTATGAAGACTGTTATAGATTGTCTTTTAACACTTAGAACACGACTTGGGTCAAGTGTTGCAGCAGACGGTTTTTCTCTGTTTAGTCCAACTGGTAACTATGACAGCCCTCGAAGAGATTTATCTTCCCGTGGATTTAGTTCTCCGTTAACTGTAGAAGAGAGGCAGAAGGTCTTGTCTGAGTCAAAATTTCAACGTTCATTGCGTAGTCCTGTTATGTCAGAGCCATCAGCAGGATTAATGCATCATGTTGGACATAAGTTCCATGAGGTATTTCAGGTGAAACAAGGACGCTACGCAGATCTTCCTGCTGCAAAGATCTCAGAAATGATGAAATCCAACAGTTTAGATTATCTCTTGTTGCAGAATGCTCCGACTCAGTCCCTTTTGAGCGTTGTAAATGGAATTCTTGACGAAAGCGTTGAAAGAAATAATAGTGAAATACCTCATCGTGTGGCTTGTCTACTGAGAAAAGTTGTCCAGGAAATTGAGCGCCGCATATCAACTCAAGCAGAGCATTTAAGAACTCAAAACAATCTTTTCAAGGCTCGTGAAGAGAAATACCAGTCAAGAATCAGAGTACTTGAAGCCCTTGCATCCGGAACCAATGAGGAAAGCGAGCTTTTTAGGAACCATCTTCAGCAAATGAAGAGTGAGAGGACCAAAATTGAAGAAAAAAAGAAAGCTGATGATGAGGATGTGATCAGATTGAAGAAAGAGAAGGAGCATAGTAACCTTCAAATTTCAGGACTGAAGCAAGAGTTGGATATAGCCAAAAAGACATATGACTTGCGTTGCTTGCAAATGGAAACAGACGCGAGAGGTGTTAAAGCAGCACTTGAAGAAAGGGTAAAAGACCTTGAACGCCTCTTGGCAGATTCAAGGAATAAGGTGAAAGAGCTGGAGGAAAACTCTGAGTCAAAACATCAGGTTTCTAACGAAACTTTAAAGCTTGAAGAAAAGGTAAAAGAACTTGAACGCCTCTTGGAAGCTTCAAGGAATAAAGTGAAAGAACTTGAGGAAAGCTCAGAGTCAAAATATCAGTTTTCCCAAGGTCAGCTTGAAGAGCGGGTGAAAGAACTTGAAAATCTCTTGGCAGATTCAAGGAAAAAGGAGAAAGAGCTTCAGGCAAATTCTGAGTCAAAATGTCAGAGTTGGATTAAGAAAGAGCGTGTTTACCATAACTTTATGGAATTTCAACTTGGTTCTTTGAAGGAATTGAGGCTATCTTCTGTGTCCATTAAGCAAGAAATAATGAAGGCAGAACGAAGCTACACGAGTGAATTTGATCATTTAGGAGTGAAGCTTCAAGCATTAGCAGATGCAGCCGAGAATTATCATTCACTTTTGGCTGAAAATAGGAGGCTGTTTAATGAAATTCAGGATTTAAAAGGAAACATCAGAGTATATTGCCGAATAAGACCATTTCTGCCTGGACAGAAGGAGAAAAGAACATTGGTAGAACATGTTGGTGACAATGGAGAACTGGTAGTTGCAGACCGCTCCAGACCAGGGAAAGATGGTCATCGATTGTTCAAGTTTAATAAGGTCTATGGTTCAGATGCAACTCAAGCTGAAGTATACACTGATACCCAACCGTTTATAAGATCTGTACTTGACGGATATAATGTGTGTATCTTTGCTTATGGTCAAACTGGATCTGGAAAAACCTACACAATGACTGGTCCTAACAATGCAACTAAGGAGAACTGGGGGGTCAACTATAGAGCCTTAAATGACCTTTTTGATGATTCTCAGCAAAGAAAAAGCTCCATTATGTATGAAATTGGTGTTCAAATGGTTGAAATATACAATGAACAAGTGCGCGATTTGCTATCAAGTGATGGTACTCCGAAAAAACTCGGGATTATGACTCATTCTCAACCCACTGGGTTAGCTGTGCCGGATGCTACTATGCACCCTGTCAAATCAACCTCAGATGTCATTAAATTAATGGATATAGGATTTAAGAACCGAGCTGTTAGTGCCACTGCCCTCAACGAAAGAAGTAGTCGCTCTCATAGTGTTCTTACAGTTCATGTACGAGGGACGGATCTGAAGACTAGTTCTGCTTTGGTCGGCAATCTTCATCTTGTAGATTTGGCAGGAAGTGAAAGGGTAGATCGCTCTGAGGTTACTGGAGATAGACTTAAGGAAGCACAACATATAAACAAATCATTATCTTCACTTGGAGATGTCATATTTGCTCTTGCACAGAAGAGCCCTCATGTACCATACAGAAATAGCAAGCTTACTCAAGTCCTTCAAAGCTCTCTCGGTACTCCTCTGCTCCTAGAATTGTTATTCTTTTCGTTTTCCCTGAGATTTGTCCTCAATAGCTCAAGCAACAGTTTCTGGTGGTTTACAATAGGTGGACAAGCAAAAACACTTATGTTTGTGCAGCTTAATCCTGAAACAAGTTCATATTCTGAAAGTTTAAGTACTCTAAAGTTTGCTGAGAGGGTTGCTGGAGTAGAGCTAGGGGCGGCAAAAAGCAACAAAGAGGGCAGGGATGCTAGGGAGTTGATGGAACAGGTCCTCATTTTATACATCATTTCTTGCATCACAAGCAAATTGGGGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005875 GO:0006928 GO:0007017 GO:0007018 GO:0008150 GO:0009987 GO:0015630 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032991 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH Kinesin Microtub_bd

Protein Analysis

991

Amino Acids

111.45

Weight (kDa)

7.33

Isoelectric Point (pI)

46.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	46 - 144	4.3e-12	Calponin homology (CH) domain
Microtub_bd	PF16796	582 - 742	1.7e-40	Microtubule binding
Kinesin	PF00225	602 - 890	1.5e-94	Kinesin motor domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004278)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G18410 AT1G73860 AT1G73860
fragaria_vesca	FvH4_5g12130 FvH4_5g12130
malus_domestica	MD06G1216000.v1.1 MD14G1226600.v1.1
prunus_persica	Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1 Prupe.5G221200_v2.0.a1
pyrus_communis	pycom06g19260 pycom14g18800
rosa_chinensis	RchiOBHm_Chr7g0185441 RchiOBHm_Chr7g0185451
rosa_laevigata	RLG00000004912
rosa_multiflora	Rmu_sc0010190.1_g000028
rosa_roxburghii	Rroxscaffold_3G00268930
rosa_rugosa	Rorug06G0469100 Rorug06G0469200 Rorug06G0469300 Rorug06G0469400 Rorug06G0469500
rosa_samantha	Rh7AG073500 Rh7BG074100 Rh7CG074300 Rh7DG074200
rosa_wichuraiana	Rw7G006170

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1994
Acc65I	GGTACC	1 cut(s)	224
AccB1I	GGYRCC	1 cut(s)	224
AccBSI	CCGCTC	2 cut(s)	1459, 1893
AccI	GTMKAC	3 cut(s)	157, 853, 1971
AccII	CGCG	2 cut(s)	1208, 2217
AccIII	TCCGGA	1 cut(s)	989
AciI	CCGC	6 cut(s)	124, 374, 886, 1459, 1891, 2876
AclI	AACGTT	1 cut(s)	586
AclWI	GGATC	5 cut(s)	279, 384, 397, 2056, 2451
AcsI	RAATTY	7 cut(s)	578, 795, 1131, 1522, 1583, 1673, 1767
AcuI	CTGAAG	7 cut(s)	135, 174, 1013, 1163, 1499, 1986, 2468
AfaI	GTAC	9 cut(s)	103, 226, 972, 2004, 2239, 2433, 2622, 2671, 2833
AfiI	CCNNNNNNNGG	4 cut(s)	71, 170, 623, 1902
AflII	CTTAAG	1 cut(s)	2540
AflIII	ACRYGT	1 cut(s)	1855
AhlI	ACTAGT	1 cut(s)	2453
AjnI	CCWGG	3 cut(s)	870, 1822, 1900
AjuI	GAANNNNNNNTTGG	6 cut(s)	1136, 1168, 1575, 1607, 1829, 1861
Alw26I	GTCTC	1 cut(s)	104
AlwI	GGATC	5 cut(s)	279, 384, 397, 2056, 2451
AlwNI	CAGNNNCTG	2 cut(s)	185, 2745
Ama87I	CYCGRG	1 cut(s)	2252
Aor13HI	TCCGGA	1 cut(s)	989
AoxI	GGCC	1 cut(s)	352
ApeKI	GCWGC	6 cut(s)	335, 452, 701, 1223, 1715, 2791
ApoI	RAATTY	7 cut(s)	578, 795, 1131, 1522, 1583, 1673, 1767
ArsI	GACNNNNNNTTYG	2 cut(s)	1514, 1546
AseI	ATTAAT	2 cut(s)	629, 2342
Asp718I	GGTACC	1 cut(s)	224
AspLEI	GCGC	2 cut(s)	885, 2217
AspS9I	GGNCC	3 cut(s)	1050, 2075, 2926
AsuHPI	GGTGA	5 cut(s)	119, 682, 1294, 1474, 1874
AsuNHI	GCTAGC	1 cut(s)	127
AvaI	CYCGRG	1 cut(s)	2252
AvaII	GGWCC	3 cut(s)	1050, 2075, 2926
BanI	GGYRCC	1 cut(s)	224
BanII	GRGCYC	1 cut(s)	2615
BauI	CACGAG	2 cut(s)	939, 1666
BbsI	GAAGAC	2 cut(s)	404, 2456
BbvI	GCAGC	6 cut(s)	322, 464, 688, 1235, 1727, 2803
BccI	CCATC	6 cut(s)	215, 625, 1032, 1904, 2228, 2911
BciT130I	CCWGG	3 cut(s)	872, 1824, 1902
BclI	TGATCA	2 cut(s)	1092, 1678
BcoDI	GTCTC	1 cut(s)	104
BcuI	ACTAGT	1 cut(s)	2453
BfaI	CTAG	6 cut(s)	128, 380, 2454, 2684, 2870, 2907
BfmI	CTRYAG	2 cut(s)	543, 2113
BfoI	RGCGCY	1 cut(s)	886
BfrI	CTTAAG	1 cut(s)	2540
BglII	AGATCT	3 cut(s)	691, 708, 1997
BisI	GCNGC	9 cut(s)	125, 336, 453, 702, 886, 1224, 1716, 2792, 2877
BlsI	GCNGC	9 cut(s)	126, 337, 454, 703, 887, 1225, 1717, 2793, 2878
BmcAI	AGTACT	2 cut(s)	972, 2833
Bme1390I	CCNGG	3 cut(s)	872, 1824, 1902
Bme18I	GGWCC	3 cut(s)	1050, 2075, 2926
BmeT110I	CYCGRG	1 cut(s)	2252
BmgT120I	GGNCC	3 cut(s)	1050, 2075, 2926
BmiI	GGNNCC	4 cut(s)	46, 226, 994, 1022
BmrFI	CCNGG	3 cut(s)	872, 1824, 1902
BmrI	ACTGGG	3 cut(s)	355, 2110, 2289
BmsI	GCATC	7 cut(s)	643, 995, 1702, 1942, 2287, 2893, 2961
BmtI	GCTAGC	1 cut(s)	131
BmuI	ACTGGG	3 cut(s)	355, 2110, 2289
BoxI	GACNNNNGTC	1 cut(s)	439
BpiI	GAAGAC	2 cut(s)	404, 2456
BplI	GAGNNNNNCTC	4 cut(s)	1286, 1318, 1400, 1432
BpmI	CTGGAG	4 cut(s)	1313, 1879, 2550, 2880
BpuEI	CTTGAG	7 cut(s)	40, 223, 882, 1427, 1944, 2631, 2716
Bsa29I	ATCGAT	1 cut(s)	1918
BsaBI	GATNNNNATC	1 cut(s)	1914
BsaJI	CCNNGG	3 cut(s)	520, 1441, 1901
BsaWI	WCCGGW	1 cut(s)	989
BsaXI	ACNNNNNCTCC	2 cut(s)	1863, 1893
Bsc4I	CCNNNNNNNGG	4 cut(s)	71, 170, 623, 1902
Bse3DI	GCAATG	1 cut(s)	590
Bse8I	GATNNNNATC	1 cut(s)	1914
BseAI	TCCGGA	1 cut(s)	989
BseBI	CCWGG	3 cut(s)	872, 1824, 1902
BseCI	ATCGAT	1 cut(s)	1918
BseDI	CCNNGG	3 cut(s)	520, 1441, 1901
BseGI	GGATG	4 cut(s)	986, 1093, 2302, 2908
BseJI	GATNNNNATC	1 cut(s)	1914
BseLI	CCNNNNNNNGG	4 cut(s)	71, 170, 623, 1902
BseMI	GCAATG	1 cut(s)	590
BseRI	GAGGAG	1 cut(s)	2664
BseXI	GCAGC	6 cut(s)	322, 464, 688, 1235, 1727, 2803
BsgI	GTGCAG	1 cut(s)	2810
Bsh1236I	CGCG	2 cut(s)	1208, 2217
BshFI	GGCC	1 cut(s)	354
BshNI	GGYRCC	1 cut(s)	224
BshVI	ATCGAT	1 cut(s)	1918
BsiHKCI	CYCGRG	1 cut(s)	2252
BsiSI	CCGG	2 cut(s)	990, 2294
BslFI	GGGAC	2 cut(s)	757, 2452
BslI	CCNNNNNNNGG	4 cut(s)	71, 170, 623, 1902
BsmAI	GTCTC	1 cut(s)	104
BsmFI	GGGAC	2 cut(s)	757, 2452
BsmI	GAATGC	1 cut(s)	763
BsnI	GGCC	1 cut(s)	354
BsoBI	CYCGRG	1 cut(s)	2252
Bsp1286I	GDGCHC	1 cut(s)	2615
Bsp13I	TCCGGA	1 cut(s)	989
BspACI	CCGC	6 cut(s)	124, 374, 886, 1459, 1891, 2876
BspANI	GGCC	1 cut(s)	354
BspDI	ATCGAT	1 cut(s)	1918
BspEI	TCCGGA	1 cut(s)	989
BspFNI	CGCG	2 cut(s)	1208, 2217
BspLI	GGNNCC	4 cut(s)	46, 226, 994, 1022
BspOI	GCTAGC	1 cut(s)	131
BspPI	GGATC	5 cut(s)	279, 384, 397, 2056, 2451
BspQI	GCTCTTC	2 cut(s)	1449, 2603
BspT107I	GGYRCC	1 cut(s)	224
BspTI	CTTAAG	1 cut(s)	2540
BsrBI	CCGCTC	2 cut(s)	1459, 1893
BsrDI	GCAATG	1 cut(s)	590
BssECI	CCNNGG	3 cut(s)	520, 1441, 1901
BssNAI	GTATAC	2 cut(s)	158, 1972
BssSI	CACGAG	2 cut(s)	939, 1666
BssT1I	CCWWGG	1 cut(s)	1441
Bst1107I	GTATAC	2 cut(s)	158, 1972
Bst2BI	CACGAG	2 cut(s)	939, 1666
Bst2UI	CCWGG	3 cut(s)	872, 1824, 1902
Bst4CI	ACNGT	7 cut(s)	403, 461, 544, 734, 1989, 2425, 2741
Bst6I	CTCTTC	7 cut(s)	19, 499, 543, 939, 1034, 1449, 2603
BstAFI	CTTAAG	1 cut(s)	2540
BstC8I	GCNNGC	6 cut(s)	122, 129, 356, 1010, 1190, 2639
BstDSI	CCRYGG	1 cut(s)	520
BstENI	CCTNNNNNAGG	1 cut(s)	168
BstF5I	GGATG	4 cut(s)	986, 1093, 2302, 2908
BstFNI	CGCG	2 cut(s)	1208, 2217
BstH2I	RGCGCY	1 cut(s)	886
BstHHI	GCGC	2 cut(s)	885, 2217
BstMAI	GTCTC	1 cut(s)	104
BstMWI	GCNNNNNNNGC	7 cut(s)	1189, 1456, 1715, 1961, 2221, 2375, 2958
BstNI	CCWGG	3 cut(s)	872, 1824, 1902
BstNSI	RCATGY	1 cut(s)	1859
BstPAI	GACNNNNGTC	1 cut(s)	439
BstSCI	CCNGG	3 cut(s)	870, 1822, 1900
BstSFI	CTRYAG	2 cut(s)	543, 2113
BstUI	CGCG	2 cut(s)	1208, 2217
BstV1I	GCAGC	6 cut(s)	322, 464, 688, 1235, 1727, 2803
BstV2I	GAAGAC	2 cut(s)	404, 2456
BstX2I	RGATCY	8 cut(s)	284, 376, 389, 691, 708, 1997, 2048, 2443
BstYI	RGATCY	8 cut(s)	284, 376, 389, 691, 708, 1997, 2048, 2443
BstZ17I	GTATAC	2 cut(s)	158, 1972
Bsu15I	ATCGAT	1 cut(s)	1918
BsuRI	GGCC	1 cut(s)	354
BsuTUI	ATCGAT	1 cut(s)	1918
BtgI	CCRYGG	1 cut(s)	520
BtsCI	GGATG	4 cut(s)	986, 1093, 2302, 2908
BtsI	GCAGTG	1 cut(s)	2379
BtsIMutI	CAGTG	4 cut(s)	306, 1974, 2277, 2379
Cac8I	GCNNGC	6 cut(s)	122, 129, 356, 1010, 1190, 2639
CaiI	CAGNNNCTG	2 cut(s)	185, 2745
CfoI	GCGC	2 cut(s)	885, 2217
Cfr13I	GGNCC	3 cut(s)	1050, 2075, 2926
ClaI	ATCGAT	1 cut(s)	1918
CseI	GACGC	2 cut(s)	690, 1214
Csp6I	GTAC	9 cut(s)	102, 225, 971, 2003, 2238, 2432, 2621, 2670, 2832
CviAII	CATG	5 cut(s)	638, 656, 1856, 2429, 2618
CviQI	GTAC	9 cut(s)	102, 225, 971, 2003, 2238, 2432, 2621, 2670, 2832
DraI	TTTAAA	2 cut(s)	1338, 1779
Eam1104I	CTCTTC	7 cut(s)	19, 499, 543, 939, 1034, 1449, 2603
EarI	CTCTTC	7 cut(s)	19, 499, 543, 939, 1034, 1449, 2603
EciI	GGCGGA	1 cut(s)	389
Eco130I	CCWWGG	1 cut(s)	1441
Eco147I	AGGCCT	1 cut(s)	354
Eco24I	GRGCYC	1 cut(s)	2615
Eco47I	GGWCC	3 cut(s)	1050, 2075, 2926
Eco57I	CTGAAG	7 cut(s)	135, 174, 1013, 1163, 1499, 1986, 2468
Eco88I	CYCGRG	1 cut(s)	2252
EcoNI	CCTNNNNNAGG	1 cut(s)	168
EcoO109I	RGGNCCY	1 cut(s)	2926
EcoRI	GAATTC	1 cut(s)	795
EcoRII	CCWGG	3 cut(s)	870, 1822, 1900
EcoT14I	CCWWGG	1 cut(s)	1441
EcoT22I	ATGCAT	1 cut(s)	636
EcoT38I	GRGCYC	1 cut(s)	2615
ErhI	CCWWGG	1 cut(s)	1441
FaeI	CATG	5 cut(s)	641, 659, 1859, 2432, 2621
FalI	AAGNNNNNCTT	4 cut(s)	1212, 1244, 1434, 1466
FaqI	GGGAC	2 cut(s)	757, 2452
FatI	CATG	5 cut(s)	637, 655, 1855, 2428, 2617
FauI	CCCGC	1 cut(s)	1452
FauNDI	CATATG	1 cut(s)	1174
FbaI	TGATCA	2 cut(s)	1092, 1678
FblI	GTMKAC	3 cut(s)	157, 853, 1971
Fnu4HI	GCNGC	9 cut(s)	125, 336, 453, 702, 886, 1224, 1716, 2792, 2877
FokI	GGATG	4 cut(s)	973, 1100, 2309, 2915
FriOI	GRGCYC	1 cut(s)	2615
Fsp4HI	GCNGC	9 cut(s)	125, 336, 453, 702, 886, 1224, 1716, 2792, 2877
FspBI	CTAG	6 cut(s)	128, 380, 2454, 2684, 2870, 2907
GlaI	GCGC	2 cut(s)	884, 2216
GluI	GCNGC	9 cut(s)	125, 336, 453, 702, 886, 1224, 1716, 2792, 2877
GsuI	CTGGAG	4 cut(s)	1313, 1879, 2550, 2880
HaeII	RGCGCY	1 cut(s)	886
HaeIII	GGCC	1 cut(s)	354
HapII	CCGG	2 cut(s)	990, 2294
HgaI	GACGC	2 cut(s)	690, 1214
HhaI	GCGC	2 cut(s)	885, 2217
Hin1II	CATG	5 cut(s)	641, 659, 1859, 2432, 2621
Hin6I	GCGC	2 cut(s)	883, 2215
HinP1I	GCGC	2 cut(s)	883, 2215
HincII	GTYRAC	2 cut(s)	540, 2110
HindII	GTYRAC	2 cut(s)	540, 2110
HindIII	AAGCTT	4 cut(s)	1340, 1380, 1694, 2639
HpaI	GTTAAC	1 cut(s)	540
HpaII	CCGG	2 cut(s)	990, 2294
HphI	GGTGA	5 cut(s)	119, 682, 1294, 1474, 1874
Hpy166II	GTNNAC	9 cut(s)	158, 176, 540, 854, 1567, 1972, 2110, 2754, 2765
Hpy8I	GTNNAC	9 cut(s)	158, 176, 540, 854, 1567, 1972, 2110, 2754, 2765
HpyAV	CCTTC	9 cut(s)	110, 379, 553, 1105, 1136, 1597, 1642, 1825, 2663
HpyCH4III	ACNGT	7 cut(s)	403, 461, 544, 734, 1989, 2425, 2741
HpyCH4IV	ACGT	1 cut(s)	586
HpyF10VI	GCNNNNNNNGC	7 cut(s)	1189, 1456, 1715, 1961, 2221, 2375, 2958
HpySE526I	ACGT	1 cut(s)	586
Hsp92II	CATG	5 cut(s)	641, 659, 1859, 2432, 2621
HspAI	GCGC	2 cut(s)	883, 2215
Kpn2I	TCCGGA	1 cut(s)	989
KpnI	GGTACC	1 cut(s)	228
Ksp22I	TGATCA	2 cut(s)	1092, 1678
KspAI	GTTAAC	1 cut(s)	540
LguI	GCTCTTC	2 cut(s)	1449, 2603
LmnI	GCTCC	5 cut(s)	766, 1114, 1898, 2165, 2685
Lsp1109I	GCAGC	6 cut(s)	322, 464, 688, 1235, 1727, 2803
LweI	GCATC	7 cut(s)	643, 995, 1702, 1942, 2287, 2893, 2961
MaeI	CTAG	6 cut(s)	128, 380, 2454, 2684, 2870, 2907
MaeII	ACGT	1 cut(s)	586
MaeIII	GTNAC	4 cut(s)	485, 1121, 1862, 2524
MbiI	CCGCTC	2 cut(s)	1459, 1893
MflI	RGATCY	8 cut(s)	284, 376, 389, 691, 708, 1997, 2048, 2443
MhlI	GDGCHC	1 cut(s)	2615
MlyI	GAGTC	6 cut(s)	581, 760, 1316, 1430, 1538, 2257
MmeI	TCCRAC	6 cut(s)	503, 622, 753, 788, 1134, 1526
Mph1103I	ATGCAT	1 cut(s)	636
MroI	TCCGGA	1 cut(s)	989
MslI	CAYNNNNRTG	1 cut(s)	1860
MspCI	CTTAAG	1 cut(s)	2540
MspI	CCGG	2 cut(s)	990, 2294
MspR9I	CCNGG	3 cut(s)	872, 1824, 1902
Mva1269I	GAATGC	1 cut(s)	763
MvaI	CCWGG	3 cut(s)	872, 1824, 1902
MvnI	CGCG	2 cut(s)	1208, 2217
MwoI	GCNNNNNNNGC	7 cut(s)	1189, 1456, 1715, 1961, 2221, 2375, 2958
NdeI	CATATG	1 cut(s)	1174
NheI	GCTAGC	1 cut(s)	127
NlaIII	CATG	5 cut(s)	641, 659, 1859, 2432, 2621
NlaIV	GGNNCC	4 cut(s)	46, 226, 994, 1022
NmeAIII	GCCGAG	1 cut(s)	1744
NmuCI	GTSAC	1 cut(s)	1862
NsiI	ATGCAT	1 cut(s)	636
NspI	RCATGY	1 cut(s)	1859
PceI	AGGCCT	1 cut(s)	354
PciI	ACATGT	1 cut(s)	1855
PciSI	GCTCTTC	2 cut(s)	1449, 2603
PcsI	WCGNNNNNNNCGW	1 cut(s)	592
PctI	GAATGC	1 cut(s)	763
PfeI	GAWTC	6 cut(s)	8, 344, 963, 1268, 1490, 2141
PflFI	GACNNNGTC	1 cut(s)	769
PfoI	TCCNGGA	1 cut(s)	870
PkrI	GCNGC	9 cut(s)	126, 337, 454, 703, 887, 1225, 1717, 2793, 2878
PleI	GAGTC	6 cut(s)	580, 760, 1315, 1429, 1537, 2257
PpsI	GAGTC	6 cut(s)	580, 760, 1315, 1429, 1537, 2257
PpuMI	RGGWCCY	1 cut(s)	2926
PscI	ACATGT	1 cut(s)	1855
PshAI	GACNNNNGTC	1 cut(s)	439
PshBI	ATTAAT	2 cut(s)	629, 2342
PsiI	TTATAA	1 cut(s)	1994
Psp1406I	AACGTT	1 cut(s)	586
Psp5II	RGGWCCY	1 cut(s)	2926
Psp6I	CCWGG	3 cut(s)	870, 1822, 1900
PspGI	CCWGG	3 cut(s)	870, 1822, 1900
PspN4I	GGNNCC	4 cut(s)	46, 226, 994, 1022
PspPI	GGNCC	3 cut(s)	1050, 2075, 2926
PspPPI	RGGWCCY	1 cut(s)	2926
PstNI	CAGNNNCTG	2 cut(s)	185, 2745
PsuI	RGATCY	8 cut(s)	284, 376, 389, 691, 708, 1997, 2048, 2443
PsyI	GACNNNGTC	1 cut(s)	769
RsaI	GTAC	9 cut(s)	103, 226, 972, 2004, 2239, 2433, 2622, 2671, 2833
RsaNI	GTAC	9 cut(s)	102, 225, 971, 2003, 2238, 2432, 2621, 2670, 2832
RseI	CAYNNNNRTG	1 cut(s)	1860
SapI	GCTCTTC	2 cut(s)	1449, 2603
SatI	GCNGC	9 cut(s)	125, 336, 453, 702, 886, 1224, 1716, 2792, 2877
Sau96I	GGNCC	3 cut(s)	1050, 2075, 2926
ScaI	AGTACT	2 cut(s)	972, 2833
SchI	GAGTC	6 cut(s)	581, 760, 1316, 1430, 1538, 2257
ScrFI	CCNGG	3 cut(s)	872, 1824, 1902
SduI	GDGCHC	1 cut(s)	2615
SfaNI	GCATC	7 cut(s)	643, 995, 1702, 1942, 2287, 2893, 2961
SfcI	CTRYAG	2 cut(s)	543, 2113
SinI	GGWCC	3 cut(s)	1050, 2075, 2926
SmiMI	CAYNNNNRTG	1 cut(s)	1860
SmlI	CTYRAG	8 cut(s)	55, 202, 897, 1406, 1959, 2540, 2646, 2731
SmoI	CTYRAG	8 cut(s)	55, 202, 897, 1406, 1959, 2540, 2646, 2731
SpeI	ACTAGT	1 cut(s)	2453
SseBI	AGGCCT	1 cut(s)	354
SsiI	CCGC	6 cut(s)	124, 374, 886, 1459, 1891, 2876
SspMI	CTAG	6 cut(s)	128, 380, 2454, 2684, 2870, 2907
StuI	AGGCCT	1 cut(s)	354
StyD4I	CCNGG	3 cut(s)	870, 1822, 1900
StyI	CCWWGG	1 cut(s)	1441
TaaI	ACNGT	7 cut(s)	403, 461, 544, 734, 1989, 2425, 2741
TaiI	ACGT	1 cut(s)	589
TaqI	TCGA	3 cut(s)	50, 502, 1918
TatI	WGTACW	3 cut(s)	970, 2002, 2831
TauI	GCSGC	3 cut(s)	127, 888, 2879
TfiI	GAWTC	6 cut(s)	8, 344, 963, 1268, 1490, 2141
TscAI	CASTG	4 cut(s)	306, 1981, 2284, 2386
TseFI	GTSAC	1 cut(s)	1862
TseI	GCWGC	6 cut(s)	335, 452, 701, 1223, 1715, 2791
Tsp45I	GTSAC	1 cut(s)	1862
TspGWI	ACGGA	3 cut(s)	525, 2025, 2456
TspRI	CASTG	4 cut(s)	306, 1981, 2284, 2386
Tth111I	GACNNNGTC	1 cut(s)	769
Vha464I	CTTAAG	1 cut(s)	2540
VpaK11BI	GGWCC	3 cut(s)	1050, 2075, 2926
VspI	ATTAAT	2 cut(s)	629, 2342
XagI	CCTNNNNNAGG	1 cut(s)	168
XapI	RAATTY	7 cut(s)	578, 795, 1131, 1522, 1583, 1673, 1767
XbaI	TCTAGA	1 cut(s)	379
XceI	RCATGY	1 cut(s)	1859
XmiI	GTMKAC	3 cut(s)	157, 853, 1971
XspI	CTAG	6 cut(s)	128, 380, 2454, 2684, 2870, 2907
ZrmI	AGTACT	2 cut(s)	972, 2833
Zsp2I	ATGCAT	1 cut(s)	636

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.