MD06G1229400.v1.1

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0014142
Physical Location & Seq
Forward (+)
35911866 .. 35914219
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1229400.v1.1.491

Sequence Viewer

Length: 1002 bp
ATGTCTGAAATAATCAAACTGAATCTCATTTTTCATTTTTCTCAGCAACCAAACTTCATTTTCTCGCGTTTTTTTCCCACCCTGATCCGGCGAAACCGATCGGAAGCGCCGATGGACAAGGTGGAGGAGGAGGTGGAGAAGGCGAAGAAGGAGTGGGACGAAGCGTACAGTAACGTCGTACAGCAAATAAAGGCGATCGAAGAGTACGGTGAATCTACTGGAGCTGCTACTATGGTGTCGCAGAAGGACTCGCTGCCCAGGATGAACGGGCTCGCACAAGACGGCTTGTCGTTGCTCAATTCATTGCAGTTCAAGCTCGATCTGTTCGCTCCCCAGTTGCCCACCGACGATCAGGTCCAATCCGCCAAGGCCTTGCTCGAATCCTGGAAAAATCGATCCCACAGTTTGCGGTTGAGTTTGAGGAATGCAAATTTGCAAGCAAAGGCTAACATGAGGAAAGCTGCTCAGAAAGAGAGAGAACTTCTTTTGGGAGGTGGAGAAGAGTCCACGATTCGTAGACGCAACTTACAAACAAAAGCTGGAATGACATCTGCCGCAGAAAGCATCACAGAGAGCCTTCGCCGTACTCGTCAGCTGATGGTTCAGGAAGTGGAACGAAGTGCTGGCACACTCATGACTTTCGAGGAATCTACTGGAGTACTGAAGAAGGCTGAAAGTGAATACAAGGGACACCGTTCATTGTTGATGCGAACCAGAAACCTACTCTCAACAATGCAACGACAAGATGTCATTGACAGGGTGATACTTGTGCTAGGATTCTTCTTGTTTTCTTTAGCTGTTCTATATGTCGTTTCAAAGCGTATTGGTCTACTGGCGCTGCAGAGGCAGGTCACATCTGCCATTAAGGCTGGTATGGTTGGGCGAGCAGAACTCAGACCTGGAGCTGTTGAAGATGCCGTAAATCATGCACAGGCCTATGGCAATGCAGCTCATAAAGTGGAGGAACCACTAGAAAGAGTGATGCACGACGAACTACGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Sec20

Protein Analysis

334

Amino Acids

37.62

Weight (kDa)

9.49

Isoelectric Point (pI)

54.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec20 PF03908 184 - 275 3.7e-29 Sec20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24315
fragaria_vesca FvH4_5g17100
malus_domestica MD06G1229400.v1.1
prunus_persica Prupe.5G230500_v2.0.a1
pyrus_communis pycom06g20040
rosa_chinensis RchiOBHm_Chr7g0178711
rosa_laevigata RLG00000005420
rosa_multiflora Rmu_sc0002320.1_g000020 Rmu_sc0037456.1_g000001
rosa_roxburghii Rroxscaffold_3G00274650
rosa_rugosa Rorug06G0418800 Rorug06G0418800
rosa_samantha Rh7AG018200 Rh7BG017700 Rh7CG018400 Rh7DG018300
rosa_wichuraiana Rw7G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
Acc36I ACCTGC 1 cut(s) 838
AccI GTMKAC 2 cut(s) 517, 829
AccII CGCG 1 cut(s) 67
AciI CCGC 3 cut(s) 363, 409, 555
AclWI GGATC 2 cut(s) 79, 390
AcsI RAATTY 1 cut(s) 430
AcuI CTGAAG 1 cut(s) 683
AfaI GTAC 5 cut(s) 167, 180, 206, 586, 660
AfiI CCNNNNNNNGG 1 cut(s) 87
AgsI TTSAA 3 cut(s) 313, 816, 911
AhdI GACNNNNNGTC 2 cut(s) 286, 746
AjnI CCWGG 3 cut(s) 257, 383, 898
AluBI AGCT 8 cut(s) 224, 316, 461, 539, 595, 797, 905, 950
AluI AGCT 8 cut(s) 224, 316, 461, 539, 595, 797, 905, 950
AlwI GGATC 2 cut(s) 79, 390
AoxI GGCC 2 cut(s) 369, 933
ApeKI GCWGC 5 cut(s) 224, 253, 461, 838, 947
ApoI RAATTY 1 cut(s) 430
AspLEI GCGC 2 cut(s) 109, 838
AspS9I GGNCC 1 cut(s) 355
AsuHPI GGTGA 2 cut(s) 221, 772
AvaII GGWCC 1 cut(s) 355
BanII GRGCYC 1 cut(s) 273
BbvI GCAGC 5 cut(s) 211, 240, 448, 825, 959
BccI CCATC 2 cut(s) 106, 592
BceAI ACGGC 3 cut(s) 298, 567, 902
BciT130I CCWGG 3 cut(s) 259, 385, 900
BfaI CTAG 2 cut(s) 773, 971
BfmI CTRYAG 1 cut(s) 839
BfoI RGCGCY 2 cut(s) 110, 839
BfuAI ACCTGC 1 cut(s) 838
BglI GCCNNNNNGGC 1 cut(s) 866
BisI GCNGC 6 cut(s) 225, 254, 462, 555, 839, 948
BlsI GCNGC 6 cut(s) 226, 255, 463, 556, 840, 949
BmcAI AGTACT 1 cut(s) 660
Bme1390I CCNGG 3 cut(s) 259, 385, 900
Bme18I GGWCC 1 cut(s) 355
BmeRI GACNNNNNGTC 2 cut(s) 286, 746
BmgT120I GGNCC 1 cut(s) 355
BmiI GGNNCC 1 cut(s) 966
BmrFI CCNGG 3 cut(s) 259, 385, 900
BmrI ACTGGG 1 cut(s) 328
BmsI GCATC 4 cut(s) 573, 696, 904, 972
BmuI ACTGGG 1 cut(s) 328
BplI GAGNNNNNCTC 2 cut(s) 876, 908
BpmI CTGGAG 3 cut(s) 240, 675, 921
Bsa29I ATCGAT 1 cut(s) 394
BsaJI CCNNGG 2 cut(s) 257, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 87
Bse1I ACTGG 4 cut(s) 223, 334, 658, 837
Bse3DI GCAATG 2 cut(s) 302, 949
BseBI CCWGG 3 cut(s) 259, 385, 900
BseCI ATCGAT 1 cut(s) 394
BseDI CCNNGG 2 cut(s) 257, 366
BseGI GGATG 1 cut(s) 267
BseLI CCNNNNNNNGG 1 cut(s) 87
BseMI GCAATG 2 cut(s) 302, 949
BseMII CTCAG 3 cut(s) 56, 479, 907
BseNI ACTGG 4 cut(s) 223, 334, 658, 837
BseRI GAGGAG 2 cut(s) 140, 143
BseXI GCAGC 5 cut(s) 211, 240, 448, 825, 959
Bsh1236I CGCG 1 cut(s) 67
Bsh1285I CGRYCG 2 cut(s) 101, 198
BshFI GGCC 2 cut(s) 371, 935
BshVI ATCGAT 1 cut(s) 394
BsiEI CGRYCG 2 cut(s) 101, 198
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 2 cut(s) 170, 702
BslI CCNNNNNNNGG 1 cut(s) 87
BsmFI GGGAC 2 cut(s) 170, 702
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 2 cut(s) 371, 935
Bsp1286I GDGCHC 1 cut(s) 273
Bsp143I GATC 6 cut(s) 84, 98, 195, 319, 349, 395
BspACI CCGC 3 cut(s) 363, 409, 555
BspANI GGCC 2 cut(s) 371, 935
BspCNI CTCAG 3 cut(s) 55, 478, 906
BspDI ATCGAT 1 cut(s) 394
BspFNI CGCG 1 cut(s) 67
BspHI TCATGA 1 cut(s) 633
BspLI GGNNCC 1 cut(s) 966
BspMAI CTGCAG 1 cut(s) 843
BspMI ACCTGC 1 cut(s) 838
BspPI GGATC 2 cut(s) 79, 390
BsrDI GCAATG 2 cut(s) 302, 949
BsrI ACTGG 4 cut(s) 223, 334, 658, 837
BssECI CCNNGG 2 cut(s) 257, 366
BssMI GATC 6 cut(s) 84, 98, 195, 319, 349, 395
BssT1I CCWWGG 1 cut(s) 366
Bst2UI CCWGG 3 cut(s) 259, 385, 900
Bst4CI ACNGT 4 cut(s) 170, 209, 404, 695
Bst6I CTCTTC 2 cut(s) 195, 495
BstC8I GCNNGC 4 cut(s) 273, 438, 625, 885
BstDEI CTNAG 3 cut(s) 42, 465, 893
BstF5I GGATG 1 cut(s) 267
BstFNI CGCG 1 cut(s) 67
BstH2I RGCGCY 2 cut(s) 110, 839
BstHHI GCGC 2 cut(s) 109, 838
BstKTI GATC 6 cut(s) 87, 101, 198, 322, 352, 398
BstMBI GATC 6 cut(s) 84, 98, 195, 319, 349, 395
BstMCI CGRYCG 2 cut(s) 101, 198
BstMWI GCNNNNNNNGC 3 cut(s) 313, 844, 866
BstNI CCWGG 3 cut(s) 259, 385, 900
BstSCI CCNGG 3 cut(s) 257, 383, 898
BstSFI CTRYAG 1 cut(s) 839
BstUI CGCG 1 cut(s) 67
BstV1I GCAGC 5 cut(s) 211, 240, 448, 825, 959
Bsu15I ATCGAT 1 cut(s) 394
BsuRI GGCC 2 cut(s) 371, 935
BsuTUI ATCGAT 1 cut(s) 394
BtsCI GGATG 1 cut(s) 267
BveI ACCTGC 1 cut(s) 838
Cac8I GCNNGC 4 cut(s) 273, 438, 625, 885
CciI TCATGA 1 cut(s) 633
CfoI GCGC 2 cut(s) 109, 838
Cfr13I GGNCC 1 cut(s) 355
ClaI ATCGAT 1 cut(s) 394
CseI GACGC 1 cut(s) 528
Csp6I GTAC 5 cut(s) 166, 179, 205, 585, 659
CviAII CATG 3 cut(s) 451, 634, 926
CviQI GTAC 5 cut(s) 166, 179, 205, 585, 659
DdeI CTNAG 3 cut(s) 42, 465, 893
DpnI GATC 6 cut(s) 86, 100, 197, 321, 351, 397
DpnII GATC 6 cut(s) 84, 98, 195, 319, 349, 395
DrdI GACNNNNNNGTC 1 cut(s) 353
DriI GACNNNNNGTC 2 cut(s) 286, 746
DseDI GACNNNNNNGTC 1 cut(s) 353
Eam1104I CTCTTC 2 cut(s) 195, 495
Eam1105I GACNNNNNGTC 2 cut(s) 286, 746
EarI CTCTTC 2 cut(s) 195, 495
EciI GGCGGA 1 cut(s) 352
Eco130I CCWWGG 1 cut(s) 366
Eco147I AGGCCT 2 cut(s) 371, 935
Eco24I GRGCYC 1 cut(s) 273
Eco47I GGWCC 1 cut(s) 355
Eco57I CTGAAG 1 cut(s) 683
EcoRII CCWGG 3 cut(s) 257, 383, 898
EcoT14I CCWWGG 1 cut(s) 366
EcoT38I GRGCYC 1 cut(s) 273
ErhI CCWWGG 1 cut(s) 366
FaeI CATG 3 cut(s) 454, 637, 929
FaiI YATR 9 cut(s) 233, 452, 635, 805, 807, 875, 927, 939, 954
FaqI GGGAC 2 cut(s) 170, 702
FatI CATG 3 cut(s) 450, 633, 925
FblI GTMKAC 2 cut(s) 517, 829
Fnu4HI GCNGC 6 cut(s) 225, 254, 462, 555, 839, 948
FokI GGATG 1 cut(s) 274
FriOI GRGCYC 1 cut(s) 273
Fsp4HI GCNGC 6 cut(s) 225, 254, 462, 555, 839, 948
FspBI CTAG 2 cut(s) 773, 971
GlaI GCGC 2 cut(s) 108, 837
GluI GCNGC 6 cut(s) 225, 254, 462, 555, 839, 948
GsuI CTGGAG 3 cut(s) 240, 675, 921
HaeII RGCGCY 2 cut(s) 110, 839
HaeIII GGCC 2 cut(s) 371, 935
HapII CCGG 1 cut(s) 88
HgaI GACGC 1 cut(s) 528
HhaI GCGC 2 cut(s) 109, 838
Hin1II CATG 3 cut(s) 454, 637, 929
Hin6I GCGC 2 cut(s) 107, 836
HinP1I GCGC 2 cut(s) 107, 836
HinfI GANTC 8 cut(s) 22, 212, 248, 380, 503, 511, 647, 777
HpaII CCGG 1 cut(s) 88
HphI GGTGA 2 cut(s) 221, 772
Hpy166II GTNNAC 3 cut(s) 507, 518, 830
Hpy188I TCNGA 4 cut(s) 7, 103, 468, 896
Hpy188III TCNNGA 2 cut(s) 605, 634
Hpy8I GTNNAC 3 cut(s) 507, 518, 830
Hpy99I CGWCG 3 cut(s) 179, 350, 992
HpyAV CCTTC 5 cut(s) 133, 142, 238, 587, 661
HpyCH4III ACNGT 4 cut(s) 170, 209, 404, 695
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 8 cut(s) 307, 428, 436, 736, 841, 929, 947, 985
HpyF10VI GCNNNNNNNGC 3 cut(s) 313, 844, 866
HpyF3I CTNAG 3 cut(s) 42, 465, 893
HpySE526I ACGT 1 cut(s) 174
Hsp92II CATG 3 cut(s) 454, 637, 929
HspAI GCGC 2 cut(s) 107, 836
Kzo9I GATC 6 cut(s) 84, 98, 195, 319, 349, 395
LmnI GCTCC 3 cut(s) 221, 334, 902
Lsp1109I GCAGC 5 cut(s) 211, 240, 448, 825, 959
LweI GCATC 4 cut(s) 573, 696, 904, 972
MaeI CTAG 2 cut(s) 773, 971
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 2 cut(s) 170, 850
MalI GATC 6 cut(s) 86, 100, 197, 321, 351, 397
MboI GATC 6 cut(s) 84, 98, 195, 319, 349, 395
MboII GAAGA 6 cut(s) 157, 212, 512, 676, 772, 923
MhlI GDGCHC 1 cut(s) 273
MluCI AATT 2 cut(s) 298, 430
MlyI GAGTC 2 cut(s) 242, 512
MnlI CCTC 9 cut(s) 118, 121, 124, 414, 447, 485, 637, 837, 955
MseI TTAA 1 cut(s) 864
MslI CAYNNNNRTG 1 cut(s) 632
MspA1I CMGCKG 1 cut(s) 595
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 3 cut(s) 259, 385, 900
Mva1269I GAATGC 1 cut(s) 430
MvaI CCWGG 3 cut(s) 259, 385, 900
MvnI CGCG 1 cut(s) 67
MwoI GCNNNNNNNGC 3 cut(s) 313, 844, 866
NdeII GATC 6 cut(s) 84, 98, 195, 319, 349, 395
NlaIII CATG 3 cut(s) 454, 637, 929
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 1 cut(s) 850
PagI TCATGA 1 cut(s) 633
PceI AGGCCT 2 cut(s) 371, 935
PcsI WCGNNNNNNNCGW 2 cut(s) 107, 586
PctI GAATGC 1 cut(s) 430
PfeI GAWTC 6 cut(s) 22, 212, 380, 511, 647, 777
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 6 cut(s) 226, 255, 463, 556, 840, 949
Ple19I CGATCG 2 cut(s) 101, 198
PleI GAGTC 2 cut(s) 242, 511
PpsI GAGTC 2 cut(s) 242, 511
Psp6I CCWGG 3 cut(s) 257, 383, 898
PspGI CCWGG 3 cut(s) 257, 383, 898
PspN4I GGNNCC 1 cut(s) 966
PspPI GGNCC 1 cut(s) 355
PstI CTGCAG 1 cut(s) 843
PvuI CGATCG 2 cut(s) 101, 198
PvuII CAGCTG 1 cut(s) 595
RsaI GTAC 5 cut(s) 167, 180, 206, 586, 660
RsaNI GTAC 5 cut(s) 166, 179, 205, 585, 659
RseI CAYNNNNRTG 1 cut(s) 632
SaqAI TTAA 1 cut(s) 864
SatI GCNGC 6 cut(s) 225, 254, 462, 555, 839, 948
Sau3AI GATC 6 cut(s) 84, 98, 195, 319, 349, 395
Sau96I GGNCC 1 cut(s) 355
ScaI AGTACT 1 cut(s) 660
SchI GAGTC 2 cut(s) 242, 512
ScrFI CCNGG 3 cut(s) 259, 385, 900
SduI GDGCHC 1 cut(s) 273
SfaNI GCATC 4 cut(s) 573, 696, 904, 972
SfcI CTRYAG 1 cut(s) 839
SinI GGWCC 1 cut(s) 355
SmiMI CAYNNNNRTG 1 cut(s) 632
Sse9I AATT 2 cut(s) 298, 430
SseBI AGGCCT 2 cut(s) 371, 935
SsiI CCGC 3 cut(s) 363, 409, 555
SspMI CTAG 2 cut(s) 773, 971
StuI AGGCCT 2 cut(s) 371, 935
StyD4I CCNGG 3 cut(s) 257, 383, 898
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 4 cut(s) 170, 209, 404, 695
TaiI ACGT 1 cut(s) 177
TaqI TCGA 5 cut(s) 198, 318, 378, 394, 642
TasI AATT 2 cut(s) 298, 430
TatI WGTACW 1 cut(s) 658
TauI GCSGC 1 cut(s) 557
TfiI GAWTC 6 cut(s) 22, 212, 380, 511, 647, 777
Tru1I TTAA 1 cut(s) 864
Tru9I TTAA 1 cut(s) 864
TseFI GTSAC 1 cut(s) 850
TseI GCWGC 5 cut(s) 224, 253, 461, 838, 947
Tsp45I GTSAC 1 cut(s) 850
TspDTI ATGAA 5 cut(s) 23, 46, 278, 291, 687
VpaK11BI GGWCC 1 cut(s) 355
XapI RAATTY 1 cut(s) 430
XmiI GTMKAC 2 cut(s) 517, 829
XspI CTAG 2 cut(s) 773, 971
ZrmI AGTACT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.