Rw7G001470

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0014142
Physical Location & Seq
Reverse (-)
1218798 .. 1220974
2177 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G001470.1

Sequence Viewer

Length: 864 bp
ATGGACAAGGTGGAGCAGGAGGTGGAGAAGGTGAAGAAGGAGTGGGACGACACCTACAACAATGTCGTAGTTCAAATCAAAGCCATCGAGAACTACGGCAAGTCGCCTTCTTCTTCTACCACCGGTGCGGAGCTAGAGCCGAAGAAGGACTCGCTGCCCAGATTGAAGGGCCTCGCGCAAGACGGCTTGTCGTTGCTCAATTCTCTGCAGTTCAAGCTCGATCTGTTCGCTCCTCAATTGCCGACCGACGATCAGGTCCAGTCCGCCAAGGCCTTGCTCGAGTCCTGGAAAAATCGATCCCAGAGTTTGAGGTTGAGTTTGAGGAATGCGAATTTGCAAGCGAATGAGAATATTAGGAAAGCTGCTCAAAAAGAGAGGGAACTTCTATTGGGAGGTGGAGGAGAGTCCACAACTCGCAGGCGAAACTTGCAGACAAAGGCTGGCATGACATCTGCCGCAGAAAGCATCACAGAGAGCCTTAGGCGGACTCGTCAACTAATGGTTCAGGAGGTGGAAAGGAGCGCAGGCACACTCCAGACTTTCGAGGAATCTACTGGAGTACTGAAGAAGGCTGAGACTGAATACAAAGGACACCGGAGTTTGTTAATGCGCACCAGAAACCTACTATCCACAATGCAACTGGGATTTTTCCTGTTTACATGTGCTGTTCTTTATGTTGTTTCAAAGCGCATTGGTATACTGACGTTGCAGAGGAAGGTCACAGCTGCTTTCAAGGCCGGTATGGTTGGGAAAGAACTCCAGCCTGGAGCTGTTGGCAACGGTGTAAATCATGCCCCGGTCCATGACAATGTAGTTCATAAAGTTCAGGAACCTTTAGAAAGATTAATGCACGATGAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Sec20

Protein Analysis

287

Amino Acids

32.01

Weight (kDa)

9.47

Isoelectric Point (pI)

52.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec20 PF03908 151 - 231 8.2e-17 Sec20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24315
fragaria_vesca FvH4_5g17100
malus_domestica MD06G1229400.v1.1
prunus_persica Prupe.5G230500_v2.0.a1
pyrus_communis pycom06g20040
rosa_chinensis RchiOBHm_Chr7g0178711
rosa_laevigata RLG00000005420
rosa_multiflora Rmu_sc0002320.1_g000020 Rmu_sc0037456.1_g000001
rosa_roxburghii Rroxscaffold_3G00274650
rosa_rugosa Rorug06G0418800 Rorug06G0418800
rosa_samantha Rh7AG018200 Rh7BG017700 Rh7CG018400 Rh7DG018300
rosa_wichuraiana Rw7G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
Acc16I TGCGCA 1 cut(s) 611
AccI GTMKAC 1 cut(s) 697
AccII CGCG 1 cut(s) 176
AciI CCGC 4 cut(s) 128, 264, 456, 484
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 1 cut(s) 561
AflIII ACRYGT 1 cut(s) 659
AgeI ACCGGT 1 cut(s) 122
AgsI TTSAA 5 cut(s) 74, 166, 214, 684, 733
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 2 cut(s) 284, 763
AluBI AGCT 5 cut(s) 133, 217, 362, 725, 770
AluI AGCT 5 cut(s) 133, 217, 362, 725, 770
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 3 cut(s) 169, 270, 735
ApeKI GCWGC 3 cut(s) 154, 362, 725
ApoI RAATTY 1 cut(s) 331
AseI ATTAAT 1 cut(s) 845
AsiGI ACCGGT 1 cut(s) 122
AspLEI GCGC 4 cut(s) 178, 524, 612, 690
AspS9I GGNCC 3 cut(s) 169, 256, 799
AsuC2I CCSGG 1 cut(s) 797
AsuHPI GGTGA 1 cut(s) 43
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 2 cut(s) 256, 799
AxyI CCTNAGG 1 cut(s) 479
BbvI GCAGC 3 cut(s) 141, 349, 712
BccI CCATC 1 cut(s) 92
BceAI ACGGC 2 cut(s) 112, 199
BciT130I CCWGG 2 cut(s) 286, 765
BcnI CCSGG 1 cut(s) 797
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 1 cut(s) 206
BisI GCNGC 4 cut(s) 155, 363, 456, 726
BlsI GCNGC 4 cut(s) 156, 364, 457, 727
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 3 cut(s) 286, 765, 797
Bme18I GGWCC 2 cut(s) 256, 799
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 169, 256, 799
BmiI GGNNCC 1 cut(s) 831
BmrFI CCNGG 3 cut(s) 286, 765, 797
BmrI ACTGGG 1 cut(s) 650
BmsI GCATC 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 650
BpmI CTGGAG 4 cut(s) 518, 576, 743, 786
BpuMI CCSGG 1 cut(s) 797
Bsa29I ATCGAT 1 cut(s) 295
BsaJI CCNNGG 2 cut(s) 267, 795
BsaWI WCCGGW 2 cut(s) 122, 594
Bse118I RCCGGY 2 cut(s) 122, 737
Bse1I ACTGG 3 cut(s) 259, 559, 645
Bse21I CCTNAGG 1 cut(s) 479
BseBI CCWGG 2 cut(s) 286, 765
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 267, 795
BseMII CTCAG 1 cut(s) 564
BseNI ACTGG 3 cut(s) 259, 559, 645
BseRI GAGGAG 2 cut(s) 222, 414
BseXI GCAGC 3 cut(s) 141, 349, 712
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 171, 272, 737
BshTI ACCGGT 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 4 cut(s) 123, 595, 738, 797
BslFI GGGAC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 3 cut(s) 171, 272, 737
BsoBI CYCGRG 1 cut(s) 278
Bsp143I GATC 3 cut(s) 220, 250, 296
BspACI CCGC 4 cut(s) 128, 264, 456, 484
BspANI GGCC 3 cut(s) 171, 272, 737
BspCNI CTCAG 1 cut(s) 565
BspDI ATCGAT 1 cut(s) 295
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 831
BspMAI CTGCAG 1 cut(s) 210
BspPI GGATC 1 cut(s) 291
BsrFI RCCGGY 2 cut(s) 122, 737
BsrI ACTGG 3 cut(s) 259, 559, 645
BssAI RCCGGY 2 cut(s) 122, 737
BssECI CCNNGG 2 cut(s) 267, 795
BssMI GATC 3 cut(s) 220, 250, 296
BssNAI GTATAC 1 cut(s) 698
BssT1I CCWWGG 1 cut(s) 267
Bst1107I GTATAC 1 cut(s) 698
Bst2UI CCWGG 2 cut(s) 286, 765
Bst4CI ACNGT 1 cut(s) 782
BstC8I GCNNGC 4 cut(s) 339, 419, 442, 526
BstDEI CTNAG 2 cut(s) 479, 573
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 4 cut(s) 178, 524, 612, 690
BstKTI GATC 3 cut(s) 223, 253, 299
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 3 cut(s) 220, 250, 296
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 3 cut(s) 214, 427, 734
BstNI CCWGG 2 cut(s) 286, 765
BstNSI RCATGY 1 cut(s) 663
BstSCI CCNGG 3 cut(s) 284, 763, 795
BstSFI CTRYAG 1 cut(s) 206
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 3 cut(s) 141, 349, 712
BstZ17I GTATAC 1 cut(s) 698
Bsu15I ATCGAT 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 3 cut(s) 171, 272, 737
BsuTUI ATCGAT 1 cut(s) 295
Cac8I GCNNGC 4 cut(s) 339, 419, 442, 526
CfoI GCGC 4 cut(s) 178, 524, 612, 690
Cfr10I RCCGGY 2 cut(s) 122, 737
Cfr13I GGNCC 3 cut(s) 169, 256, 799
ClaI ATCGAT 1 cut(s) 295
Csp6I GTAC 1 cut(s) 560
CspAI ACCGGT 1 cut(s) 122
CviAII CATG 4 cut(s) 445, 660, 791, 803
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 2 cut(s) 479, 573
DpnI GATC 3 cut(s) 222, 252, 298
DpnII GATC 3 cut(s) 220, 250, 296
DrdI GACNNNNNNGTC 1 cut(s) 254
DriI GACNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 254
Eam1105I GACNNNNNGTC 1 cut(s) 187
EciI GGCGGA 2 cut(s) 253, 499
Eco130I CCWWGG 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 272
Eco47I GGWCC 2 cut(s) 256, 799
Eco57I CTGAAG 1 cut(s) 584
Eco81I CCTNAGG 1 cut(s) 479
Eco88I CYCGRG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 284, 763
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 4 cut(s) 448, 663, 794, 806
FaiI YATR 9 cut(s) 446, 661, 675, 698, 743, 792, 804, 819, 862
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 444, 659, 790, 802
FblI GTMKAC 1 cut(s) 697
Fnu4HI GCNGC 4 cut(s) 155, 363, 456, 726
Fsp4HI GCNGC 4 cut(s) 155, 363, 456, 726
FspAI RTGCGCAY 1 cut(s) 611
FspBI CTAG 1 cut(s) 134
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 4 cut(s) 177, 523, 611, 689
GluI GCNGC 4 cut(s) 155, 363, 456, 726
GsuI CTGGAG 4 cut(s) 518, 576, 743, 786
HaeIII GGCC 3 cut(s) 171, 272, 737
HapII CCGG 4 cut(s) 123, 595, 738, 797
HhaI GCGC 4 cut(s) 178, 524, 612, 690
Hin1II CATG 4 cut(s) 448, 663, 794, 806
Hin6I GCGC 4 cut(s) 176, 522, 610, 688
HinP1I GCGC 4 cut(s) 176, 522, 610, 688
HincII GTYRAC 1 cut(s) 494
HindII GTYRAC 1 cut(s) 494
HinfI GANTC 5 cut(s) 149, 281, 404, 487, 548
HpaII CCGG 4 cut(s) 123, 595, 738, 797
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 4 cut(s) 408, 494, 657, 698
Hpy188III TCNNGA 4 cut(s) 88, 506, 535, 827
Hpy8I GTNNAC 4 cut(s) 408, 494, 657, 698
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 7 cut(s) 22, 31, 117, 139, 160, 562, 709
HpyCH4III ACNGT 1 cut(s) 782
HpyCH4IV ACGT 1 cut(s) 704
HpyCH4V TGCA 6 cut(s) 208, 337, 430, 637, 709, 850
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 427, 734
HpyF3I CTNAG 2 cut(s) 479, 573
HpySE526I ACGT 1 cut(s) 704
Hsp92II CATG 4 cut(s) 448, 663, 794, 806
HspAI GCGC 4 cut(s) 176, 522, 610, 688
Kzo9I GATC 3 cut(s) 220, 250, 296
LmnI GCTCC 5 cut(s) 13, 130, 235, 519, 767
Lsp1109I GCAGC 3 cut(s) 141, 349, 712
LweI GCATC 1 cut(s) 474
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 704
MaeIII GTNAC 1 cut(s) 718
MalI GATC 3 cut(s) 222, 252, 298
MboI GATC 3 cut(s) 220, 250, 296
MboII GAAGA 5 cut(s) 46, 102, 105, 154, 577
MfeI CAATTG 1 cut(s) 236
MluCI AATT 3 cut(s) 199, 236, 331
MlyI GAGTC 4 cut(s) 143, 290, 413, 481
MseI TTAA 2 cut(s) 605, 845
MslI CAYNNNNRTG 1 cut(s) 807
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 4 cut(s) 123, 595, 738, 797
MspR9I CCNGG 3 cut(s) 286, 765, 797
MunI CAATTG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 2 cut(s) 286, 765
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 214, 427, 734
NciI CCSGG 1 cut(s) 797
NdeII GATC 3 cut(s) 220, 250, 296
NlaIII CATG 4 cut(s) 448, 663, 794, 806
NlaIV GGNNCC 1 cut(s) 831
NmuCI GTSAC 1 cut(s) 718
NsbI TGCGCA 1 cut(s) 611
NspI RCATGY 1 cut(s) 663
PaeR7I CTCGAG 1 cut(s) 278
PceI AGGCCT 1 cut(s) 272
PciI ACATGT 1 cut(s) 659
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 284
PinAI ACCGGT 1 cut(s) 122
PkrI GCNGC 4 cut(s) 156, 364, 457, 727
PleI GAGTC 4 cut(s) 143, 289, 412, 481
PpsI GAGTC 4 cut(s) 143, 289, 412, 481
PscI ACATGT 1 cut(s) 659
PshBI ATTAAT 1 cut(s) 845
Psp6I CCWGG 2 cut(s) 284, 763
PspGI CCWGG 2 cut(s) 284, 763
PspN4I GGNNCC 1 cut(s) 831
PspPI GGNCC 3 cut(s) 169, 256, 799
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 807
SaqAI TTAA 2 cut(s) 605, 845
SatI GCNGC 4 cut(s) 155, 363, 456, 726
Sau3AI GATC 3 cut(s) 220, 250, 296
Sau96I GGNCC 3 cut(s) 169, 256, 799
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 4 cut(s) 143, 290, 413, 481
ScrFI CCNGG 3 cut(s) 286, 765, 797
SfaNI GCATC 1 cut(s) 474
SfcI CTRYAG 1 cut(s) 206
Sfr274I CTCGAG 1 cut(s) 278
SgrAI CRCCGGYG 1 cut(s) 122
SinI GGWCC 2 cut(s) 256, 799
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 3 cut(s) 199, 236, 331
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 4 cut(s) 128, 264, 456, 484
SspI AATATT 1 cut(s) 352
SspMI CTAG 1 cut(s) 134
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 284, 763, 795
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 782
TaiI ACGT 1 cut(s) 707
TaqI TCGA 5 cut(s) 87, 219, 279, 295, 543
TaqII GACCGA 1 cut(s) 260
TasI AATT 3 cut(s) 199, 236, 331
TatI WGTACW 1 cut(s) 559
TauI GCSGC 1 cut(s) 458
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 2 cut(s) 605, 845
Tru9I TTAA 2 cut(s) 605, 845
TseFI GTSAC 1 cut(s) 718
TseI GCWGC 3 cut(s) 154, 362, 725
Tsp45I GTSAC 1 cut(s) 718
TspDTI ATGAA 1 cut(s) 806
VpaK11BI GGWCC 2 cut(s) 256, 799
VspI ATTAAT 1 cut(s) 845
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 663
XcmI CCANNNNNNNNNTGG 1 cut(s) 637
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 697
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.