RchiOBHm_Chr7g0178711

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0014142
Physical Location & Seq
Reverse (-)
1295241 .. 1297728
2488 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ15927

Sequence Viewer

Length: 897 bp
ATGGACAAGGTGGAGCAGGAGGTGGAGAAGGTGAAGAAGGAGTGGGACGACACCTACAACAATGTCGTAGATCAAATCAAAGCCATCGAGAACTACGGCAAGTCACCTTCTTCTTCTACTTCCACTACGGAGCTAGAGCCGAAGAAGGACTCGCTGCCCAGATTGAAGGGCCTCGCGCAAGACGGCTTGTCGTTGCTCAATTCTCTGCAGTTCAAGCTCGATCTGTTCGCTCCTCAATTGCCGACCGACGATCAGGTCCAGTCCGCCAAGGCCTTGCTCGAGTCCTGGAAAAATCGATCCCAGAGTTTGAGGTTGAGTTTGAGGAATGCGAATTTGCAAGCGAATGAGAATATTAGGAAAGCTGCTCAGAAAGAGAGGGAACTTCTATTGGGAGGTGGAGGAGAGTCCACAACTCGCAGGCGAAACTTGCAGACAAAGGCTGGCATGACATCTGCTGCAGAAAGCATTACAGAGAGCCTTAGGCGGACTCGTCAACTAATGGTTCAGGAGGTGGAAAGGAGCGCAGGCACACTCCAGACTTTCGAGGAATCTACTGGAGTACTGAAGAAGGCTGAGACTGAATACAAAGGACACCGGAGTTTGTTAATGCGCACCAGAAACCTACTATCCACAATGCAACGCCAAGATATCATTGACAGGGTGATAATTTCAGTGGGATTTTTCCTGTTTACATGTGCTGTTCTTTATGTTGTTTCAAAGCGCATTGGTATACTGACGTTGCAGAGGAAGGTCACAGCTGCTTTCAAGGCCGGTATGGTTGGGAAAGAACTCCAGCCTGGAGCTGTTGGCAACGGTGTAAATCATGCCCCGGTCCATGACAATGTAGTTCATAAAGTTCAGGAACCTTTAGAAAGATTAATGCACGATGAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Sec20

Protein Analysis

298

Amino Acids

33.38

Weight (kDa)

9.38

Isoelectric Point (pI)

52.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec20 PF03908 151 - 242 4.6e-29 Sec20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24315
fragaria_vesca FvH4_5g17100
malus_domestica MD06G1229400.v1.1
prunus_persica Prupe.5G230500_v2.0.a1
pyrus_communis pycom06g20040
rosa_chinensis RchiOBHm_Chr7g0178711
rosa_laevigata RLG00000005420
rosa_multiflora Rmu_sc0002320.1_g000020 Rmu_sc0037456.1_g000001
rosa_roxburghii Rroxscaffold_3G00274650
rosa_rugosa Rorug06G0418800 Rorug06G0418800
rosa_samantha Rh7AG018200 Rh7BG017700 Rh7CG018400 Rh7DG018300
rosa_wichuraiana Rw7G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 254
Acc16I TGCGCA 1 cut(s) 611
AccI GTMKAC 1 cut(s) 730
AccII CGCG 1 cut(s) 176
AciI CCGC 2 cut(s) 264, 484
AclWI GGATC 1 cut(s) 291
AcsI RAATTY 1 cut(s) 331
AcuI CTGAAG 1 cut(s) 584
AfaI GTAC 1 cut(s) 561
AflIII ACRYGT 1 cut(s) 692
AgsI TTSAA 4 cut(s) 166, 214, 717, 766
AhdI GACNNNNNGTC 1 cut(s) 187
AjnI CCWGG 2 cut(s) 284, 796
AluBI AGCT 5 cut(s) 133, 217, 362, 758, 803
AluI AGCT 5 cut(s) 133, 217, 362, 758, 803
Alw26I GTCTC 1 cut(s) 569
AlwI GGATC 1 cut(s) 291
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 3 cut(s) 169, 270, 768
ApeKI GCWGC 4 cut(s) 154, 362, 455, 758
ApoI RAATTY 1 cut(s) 331
AseI ATTAAT 1 cut(s) 878
AspLEI GCGC 4 cut(s) 178, 524, 612, 723
AspS9I GGNCC 3 cut(s) 169, 256, 832
AsuC2I CCSGG 1 cut(s) 830
AsuHPI GGTGA 3 cut(s) 43, 96, 673
AvaI CYCGRG 1 cut(s) 278
AvaII GGWCC 2 cut(s) 256, 832
AxyI CCTNAGG 1 cut(s) 479
BbvI GCAGC 4 cut(s) 141, 349, 442, 745
BccI CCATC 1 cut(s) 92
BceAI ACGGC 2 cut(s) 112, 199
BciT130I CCWGG 2 cut(s) 286, 798
BcnI CCSGG 1 cut(s) 830
BcoDI GTCTC 1 cut(s) 569
BfaI CTAG 1 cut(s) 134
BfmI CTRYAG 2 cut(s) 206, 456
BisI GCNGC 4 cut(s) 155, 363, 456, 759
BlsI GCNGC 4 cut(s) 156, 364, 457, 760
BmcAI AGTACT 1 cut(s) 561
Bme1390I CCNGG 3 cut(s) 286, 798, 830
Bme18I GGWCC 2 cut(s) 256, 832
BmeRI GACNNNNNGTC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 3 cut(s) 169, 256, 832
BmiI GGNNCC 1 cut(s) 864
BmrFI CCNGG 3 cut(s) 286, 798, 830
BpmI CTGGAG 4 cut(s) 518, 576, 776, 819
BpuMI CCSGG 1 cut(s) 830
Bsa29I ATCGAT 1 cut(s) 295
BsaJI CCNNGG 2 cut(s) 267, 828
BsaWI WCCGGW 1 cut(s) 594
Bse118I RCCGGY 1 cut(s) 770
Bse1I ACTGG 2 cut(s) 259, 559
Bse21I CCTNAGG 1 cut(s) 479
BseBI CCWGG 2 cut(s) 286, 798
BseCI ATCGAT 1 cut(s) 295
BseDI CCNNGG 2 cut(s) 267, 828
BseMII CTCAG 2 cut(s) 380, 564
BseNI ACTGG 2 cut(s) 259, 559
BseRI GAGGAG 2 cut(s) 222, 414
BseXI GCAGC 4 cut(s) 141, 349, 442, 745
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 171, 272, 770
BshVI ATCGAT 1 cut(s) 295
BsiEI CGRYCG 1 cut(s) 246
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 3 cut(s) 595, 771, 830
BslFI GGGAC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 569
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 331
BsnI GGCC 3 cut(s) 171, 272, 770
BsoBI CYCGRG 1 cut(s) 278
Bsp143I GATC 4 cut(s) 70, 220, 250, 296
BspACI CCGC 2 cut(s) 264, 484
BspANI GGCC 3 cut(s) 171, 272, 770
BspCNI CTCAG 2 cut(s) 379, 565
BspDI ATCGAT 1 cut(s) 295
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 1 cut(s) 864
BspMAI CTGCAG 2 cut(s) 210, 460
BspPI GGATC 1 cut(s) 291
BsrFI RCCGGY 1 cut(s) 770
BsrI ACTGG 2 cut(s) 259, 559
BssAI RCCGGY 1 cut(s) 770
BssECI CCNNGG 2 cut(s) 267, 828
BssMI GATC 4 cut(s) 70, 220, 250, 296
BssNAI GTATAC 1 cut(s) 731
BssT1I CCWWGG 1 cut(s) 267
Bst1107I GTATAC 1 cut(s) 731
Bst2UI CCWGG 2 cut(s) 286, 798
Bst4CI ACNGT 1 cut(s) 815
BstC8I GCNNGC 4 cut(s) 339, 419, 442, 526
BstDEI CTNAG 3 cut(s) 366, 479, 573
BstFNI CGCG 1 cut(s) 176
BstHHI GCGC 4 cut(s) 178, 524, 612, 723
BstKTI GATC 4 cut(s) 73, 223, 253, 299
BstMAI GTCTC 1 cut(s) 569
BstMBI GATC 4 cut(s) 70, 220, 250, 296
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 3 cut(s) 214, 427, 767
BstNI CCWGG 2 cut(s) 286, 798
BstNSI RCATGY 1 cut(s) 696
BstSCI CCNGG 3 cut(s) 284, 796, 828
BstSFI CTRYAG 2 cut(s) 206, 456
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 4 cut(s) 141, 349, 442, 745
BstZ17I GTATAC 1 cut(s) 731
Bsu15I ATCGAT 1 cut(s) 295
Bsu36I CCTNAGG 1 cut(s) 479
BsuRI GGCC 3 cut(s) 171, 272, 770
BsuTUI ATCGAT 1 cut(s) 295
BtsIMutI CAGTG 1 cut(s) 678
Cac8I GCNNGC 4 cut(s) 339, 419, 442, 526
CfoI GCGC 4 cut(s) 178, 524, 612, 723
Cfr10I RCCGGY 1 cut(s) 770
Cfr13I GGNCC 3 cut(s) 169, 256, 832
ClaI ATCGAT 1 cut(s) 295
Csp6I GTAC 1 cut(s) 560
CviAII CATG 4 cut(s) 445, 693, 824, 836
CviQI GTAC 1 cut(s) 560
DdeI CTNAG 3 cut(s) 366, 479, 573
DpnI GATC 4 cut(s) 72, 222, 252, 298
DpnII GATC 4 cut(s) 70, 220, 250, 296
DrdI GACNNNNNNGTC 1 cut(s) 254
DriI GACNNNNNGTC 1 cut(s) 187
DseDI GACNNNNNNGTC 1 cut(s) 254
Eam1105I GACNNNNNGTC 1 cut(s) 187
EciI GGCGGA 2 cut(s) 253, 499
Eco130I CCWWGG 1 cut(s) 267
Eco147I AGGCCT 1 cut(s) 272
Eco32I GATATC 1 cut(s) 649
Eco47I GGWCC 2 cut(s) 256, 832
Eco57I CTGAAG 1 cut(s) 584
Eco81I CCTNAGG 1 cut(s) 479
Eco88I CYCGRG 1 cut(s) 278
EcoO109I RGGNCCY 1 cut(s) 169
EcoRII CCWGG 2 cut(s) 284, 796
EcoRV GATATC 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 4 cut(s) 448, 696, 827, 839
FaiI YATR 9 cut(s) 446, 694, 708, 731, 776, 825, 837, 852, 895
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 444, 692, 823, 835
FblI GTMKAC 1 cut(s) 730
Fnu4HI GCNGC 4 cut(s) 155, 363, 456, 759
Fsp4HI GCNGC 4 cut(s) 155, 363, 456, 759
FspAI RTGCGCAY 1 cut(s) 611
FspBI CTAG 1 cut(s) 134
FspI TGCGCA 1 cut(s) 611
GlaI GCGC 4 cut(s) 177, 523, 611, 722
GluI GCNGC 4 cut(s) 155, 363, 456, 759
GsuI CTGGAG 4 cut(s) 518, 576, 776, 819
HaeIII GGCC 3 cut(s) 171, 272, 770
HapII CCGG 3 cut(s) 595, 771, 830
HhaI GCGC 4 cut(s) 178, 524, 612, 723
Hin1II CATG 4 cut(s) 448, 696, 827, 839
Hin6I GCGC 4 cut(s) 176, 522, 610, 721
HinP1I GCGC 4 cut(s) 176, 522, 610, 721
HincII GTYRAC 1 cut(s) 494
HindII GTYRAC 1 cut(s) 494
HinfI GANTC 5 cut(s) 149, 281, 404, 487, 548
HpaII CCGG 3 cut(s) 595, 771, 830
HphI GGTGA 3 cut(s) 43, 96, 673
Hpy166II GTNNAC 4 cut(s) 408, 494, 690, 731
Hpy188I TCNGA 1 cut(s) 369
Hpy188III TCNNGA 4 cut(s) 88, 506, 535, 860
Hpy8I GTNNAC 4 cut(s) 408, 494, 690, 731
Hpy99I CGWCG 1 cut(s) 251
HpyAV CCTTC 7 cut(s) 22, 31, 117, 139, 160, 562, 742
HpyCH4III ACNGT 1 cut(s) 815
HpyCH4IV ACGT 1 cut(s) 737
HpyCH4V TGCA 7 cut(s) 208, 337, 430, 458, 637, 742, 883
HpyF10VI GCNNNNNNNGC 3 cut(s) 214, 427, 767
HpyF3I CTNAG 3 cut(s) 366, 479, 573
HpySE526I ACGT 1 cut(s) 737
Hsp92II CATG 4 cut(s) 448, 696, 827, 839
HspAI GCGC 4 cut(s) 176, 522, 610, 721
Kzo9I GATC 4 cut(s) 70, 220, 250, 296
LmnI GCTCC 5 cut(s) 13, 130, 235, 519, 800
Lsp1109I GCAGC 4 cut(s) 141, 349, 442, 745
MaeI CTAG 1 cut(s) 134
MaeII ACGT 1 cut(s) 737
MaeIII GTNAC 2 cut(s) 102, 751
MalI GATC 4 cut(s) 72, 222, 252, 298
MboI GATC 4 cut(s) 70, 220, 250, 296
MboII GAAGA 5 cut(s) 46, 102, 105, 154, 577
MfeI CAATTG 1 cut(s) 236
MluCI AATT 4 cut(s) 199, 236, 331, 666
MlyI GAGTC 4 cut(s) 143, 290, 413, 481
MseI TTAA 2 cut(s) 605, 878
MslI CAYNNNNRTG 1 cut(s) 840
MspA1I CMGCKG 1 cut(s) 758
MspI CCGG 3 cut(s) 595, 771, 830
MspR9I CCNGG 3 cut(s) 286, 798, 830
MunI CAATTG 1 cut(s) 236
Mva1269I GAATGC 1 cut(s) 331
MvaI CCWGG 2 cut(s) 286, 798
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 214, 427, 767
NciI CCSGG 1 cut(s) 830
NdeII GATC 4 cut(s) 70, 220, 250, 296
NlaIII CATG 4 cut(s) 448, 696, 827, 839
NlaIV GGNNCC 1 cut(s) 864
NmuCI GTSAC 2 cut(s) 102, 751
NsbI TGCGCA 1 cut(s) 611
NspI RCATGY 1 cut(s) 696
PaeR7I CTCGAG 1 cut(s) 278
PceI AGGCCT 1 cut(s) 272
PciI ACATGT 1 cut(s) 692
PctI GAATGC 1 cut(s) 331
PfeI GAWTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 4 cut(s) 156, 364, 457, 760
PleI GAGTC 4 cut(s) 143, 289, 412, 481
PpsI GAGTC 4 cut(s) 143, 289, 412, 481
PscI ACATGT 1 cut(s) 692
PshBI ATTAAT 1 cut(s) 878
Psp6I CCWGG 2 cut(s) 284, 796
PspGI CCWGG 2 cut(s) 284, 796
PspN4I GGNNCC 1 cut(s) 864
PspPI GGNCC 3 cut(s) 169, 256, 832
PspXI VCTCGAGB 1 cut(s) 278
PstI CTGCAG 2 cut(s) 210, 460
PvuII CAGCTG 1 cut(s) 758
RsaI GTAC 1 cut(s) 561
RsaNI GTAC 1 cut(s) 560
RseI CAYNNNNRTG 1 cut(s) 840
SaqAI TTAA 2 cut(s) 605, 878
SatI GCNGC 4 cut(s) 155, 363, 456, 759
Sau3AI GATC 4 cut(s) 70, 220, 250, 296
Sau96I GGNCC 3 cut(s) 169, 256, 832
ScaI AGTACT 1 cut(s) 561
SchI GAGTC 4 cut(s) 143, 290, 413, 481
ScrFI CCNGG 3 cut(s) 286, 798, 830
SfcI CTRYAG 2 cut(s) 206, 456
Sfr274I CTCGAG 1 cut(s) 278
SinI GGWCC 2 cut(s) 256, 832
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 840
SmlI CTYRAG 1 cut(s) 278
SmoI CTYRAG 1 cut(s) 278
Sse9I AATT 4 cut(s) 199, 236, 331, 666
SseBI AGGCCT 1 cut(s) 272
SsiI CCGC 2 cut(s) 264, 484
SspI AATATT 1 cut(s) 352
SspMI CTAG 1 cut(s) 134
StuI AGGCCT 1 cut(s) 272
StyD4I CCNGG 3 cut(s) 284, 796, 828
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 815
TaiI ACGT 1 cut(s) 740
TaqI TCGA 5 cut(s) 87, 219, 279, 295, 543
TaqII GACCGA 1 cut(s) 260
TasI AATT 4 cut(s) 199, 236, 331, 666
TatI WGTACW 1 cut(s) 559
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 2 cut(s) 605, 878
Tru9I TTAA 2 cut(s) 605, 878
TscAI CASTG 1 cut(s) 678
TseFI GTSAC 2 cut(s) 102, 751
TseI GCWGC 4 cut(s) 154, 362, 455, 758
Tsp45I GTSAC 2 cut(s) 102, 751
TspDTI ATGAA 1 cut(s) 839
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 678
VpaK11BI GGWCC 2 cut(s) 256, 832
VspI ATTAAT 1 cut(s) 878
XapI RAATTY 1 cut(s) 331
XceI RCATGY 1 cut(s) 696
XhoI CTCGAG 1 cut(s) 278
XmiI GTMKAC 1 cut(s) 730
XspI CTAG 1 cut(s) 134
ZrmI AGTACT 1 cut(s) 561
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.