Rroxscaffold_3G00274650

Vesicle transport protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0014142
Physical Location & Seq
Forward (+)
66270123 .. 66272667
2545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00274650.1

Sequence Viewer

Length: 918 bp
ATGGACAAGGTGGAGCAGGAGGTGGAGAAGGTGAAGAAGGAGTGGGACGACACCTACAACAATGTCGTAGATCAAATCAAAGCCATCGAGAACTACGGCAAGTCGCCTTCTTCTTCTTCTTCTTCTTCTTCTACTACCGGCGCGGAGCTCGAGCCGAAGAAGGACAAGGACTCGCTGCCCAGATTGAAGGGCATCGCGCAAGACGGCTTGTCGTTGCTCAATTCTCTGCAGTTCAAGCTCGATCTGTTCGCTCCTCAATTGCCGACCGACGATCAGGTCCAGTCCGCCAAGGCCTTGCTCGAGTCCTGGAAAAATCGATCCCAGAGTTTGAGGTTGAGTATGAGGAATGCGAATTTGCAAGCGAATGAGAATATTAGGAAAGCTGCTCAGAAAGAGAGGGAACTTCTATTGGGAGCTGGAGGAGAGTCCACTACTCGCAGGCGAAACTTGCAGACAAAGGCTGGCATGACATCTGCCGCAGAAAGCATCACAGAGAGCCTTAGGCGGACTCGTCAACTAATGGTTCAGGAGGTGGAAAGGAGCGCAGGCACACTCCAGACTTTCGAGGAATCTACTGGAGTACTGAAGAAAGCTGAGACTGAATACAAAGGACACCGAAGTTTGTTAATGCGTACCAGAAACCTACTATCCACAATGCAACGCCAAGATATCATTGACAGGGTGATAATTTCTGTGGGATTTTTCCTGTTTACATGTGCTGTTCTTTATGTTGTTTCGAAGCGCATTGGTATACTGACGTTGCAGAGGAAGGTTACAGCTGCTTTCAAGGCCGGTATGGTTGGGAAAGAATTCCAGCCTGGAGCTGTTGGCAACGGTGTAAATCATGCCCCGGTCTATGACAATGTAGTTCATAAAGTTCAGGAACCTTTAGAAAGATTAATGCATGATGAACTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04130 map04130
Pfam Domains
Protein Families
Sec20

Protein Analysis

305

Amino Acids

34.1

Weight (kDa)

9.35

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec20 PF03908 158 - 249 4.8e-29 Sec20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014142)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G24315
fragaria_vesca FvH4_5g17100
malus_domestica MD06G1229400.v1.1
prunus_persica Prupe.5G230500_v2.0.a1
pyrus_communis pycom06g20040
rosa_chinensis RchiOBHm_Chr7g0178711
rosa_laevigata RLG00000005420
rosa_multiflora Rmu_sc0002320.1_g000020 Rmu_sc0037456.1_g000001
rosa_roxburghii Rroxscaffold_3G00274650
rosa_rugosa Rorug06G0418800 Rorug06G0418800
rosa_samantha Rh7AG018200 Rh7BG017700 Rh7CG018400 Rh7DG018300
rosa_wichuraiana Rw7G001470

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 275
AccI GTMKAC 1 cut(s) 751
AccII CGCG 2 cut(s) 143, 197
AciI CCGC 4 cut(s) 143, 285, 477, 505
AclWI GGATC 1 cut(s) 312
AcsI RAATTY 2 cut(s) 352, 809
AcuI CTGAAG 1 cut(s) 605
AfaI GTAC 2 cut(s) 582, 634
AflIII ACRYGT 1 cut(s) 713
AgsI TTSAA 3 cut(s) 187, 235, 787
AhdI GACNNNNNGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 305, 817
AluBI AGCT 7 cut(s) 148, 238, 383, 416, 593, 779, 824
AluI AGCT 7 cut(s) 148, 238, 383, 416, 593, 779, 824
Alw21I GWGCWC 1 cut(s) 150
Alw26I GTCTC 1 cut(s) 590
AlwI GGATC 1 cut(s) 312
Ama87I CYCGRG 2 cut(s) 149, 299
AoxI GGCC 2 cut(s) 291, 789
ApeKI GCWGC 3 cut(s) 175, 383, 779
ApoI RAATTY 2 cut(s) 352, 809
AseI ATTAAT 1 cut(s) 899
Asp700I GAANNNNTTC 1 cut(s) 809
AspLEI GCGC 4 cut(s) 143, 199, 545, 744
AspS9I GGNCC 1 cut(s) 277
AsuC2I CCSGG 1 cut(s) 851
AsuHPI GGTGA 2 cut(s) 43, 694
AsuII TTCGAA 1 cut(s) 737
AvaI CYCGRG 2 cut(s) 149, 299
AvaII GGWCC 1 cut(s) 277
AxyI CCTNAGG 1 cut(s) 500
BanII GRGCYC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 150
BbvI GCAGC 3 cut(s) 162, 370, 766
BccI CCATC 1 cut(s) 92
BceAI ACGGC 2 cut(s) 112, 220
BciT130I CCWGG 2 cut(s) 307, 819
BcnI CCSGG 1 cut(s) 851
BcoDI GTCTC 1 cut(s) 590
BfmI CTRYAG 1 cut(s) 227
BisI GCNGC 4 cut(s) 176, 384, 477, 780
BlsI GCNGC 4 cut(s) 177, 385, 478, 781
BmcAI AGTACT 1 cut(s) 582
Bme1390I CCNGG 3 cut(s) 307, 819, 851
Bme18I GGWCC 1 cut(s) 277
BmeRI GACNNNNNGTC 1 cut(s) 208
BmeT110I CYCGRG 2 cut(s) 149, 299
BmgT120I GGNCC 1 cut(s) 277
BmiI GGNNCC 1 cut(s) 885
BmrFI CCNGG 3 cut(s) 307, 819, 851
BmsI GCATC 2 cut(s) 201, 495
BpmI CTGGAG 4 cut(s) 438, 539, 597, 840
Bpu14I TTCGAA 1 cut(s) 737
BpuMI CCSGG 1 cut(s) 851
Bsa29I ATCGAT 1 cut(s) 316
BsaJI CCNNGG 2 cut(s) 288, 849
Bse118I RCCGGY 2 cut(s) 137, 791
Bse1I ACTGG 2 cut(s) 280, 580
Bse21I CCTNAGG 1 cut(s) 500
BseBI CCWGG 2 cut(s) 307, 819
BseCI ATCGAT 1 cut(s) 316
BseDI CCNNGG 2 cut(s) 288, 849
BseMII CTCAG 2 cut(s) 401, 585
BseNI ACTGG 2 cut(s) 280, 580
BseRI GAGGAG 2 cut(s) 243, 435
BseXI GCAGC 3 cut(s) 162, 370, 766
Bsh1236I CGCG 2 cut(s) 143, 197
Bsh1285I CGRYCG 1 cut(s) 267
BshFI GGCC 2 cut(s) 293, 791
BshVI ATCGAT 1 cut(s) 316
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 150
BsiHKCI CYCGRG 2 cut(s) 149, 299
BsiSI CCGG 3 cut(s) 138, 792, 851
BslFI GGGAC 1 cut(s) 59
BsmAI GTCTC 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 59
BsmI GAATGC 1 cut(s) 352
BsnI GGCC 2 cut(s) 293, 791
BsoBI CYCGRG 2 cut(s) 149, 299
Bsp119I TTCGAA 1 cut(s) 737
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 70, 241, 271, 317
BspACI CCGC 4 cut(s) 143, 285, 477, 505
BspANI GGCC 2 cut(s) 293, 791
BspCNI CTCAG 2 cut(s) 400, 586
BspDI ATCGAT 1 cut(s) 316
BspFNI CGCG 2 cut(s) 143, 197
BspLI GGNNCC 1 cut(s) 885
BspMAI CTGCAG 1 cut(s) 231
BspPI GGATC 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 737
BsrFI RCCGGY 2 cut(s) 137, 791
BsrI ACTGG 2 cut(s) 280, 580
BssAI RCCGGY 2 cut(s) 137, 791
BssECI CCNNGG 2 cut(s) 288, 849
BssMI GATC 4 cut(s) 70, 241, 271, 317
BssNAI GTATAC 1 cut(s) 752
BssT1I CCWWGG 1 cut(s) 288
Bst1107I GTATAC 1 cut(s) 752
Bst2UI CCWGG 2 cut(s) 307, 819
Bst4CI ACNGT 1 cut(s) 836
BstBI TTCGAA 1 cut(s) 737
BstC8I GCNNGC 4 cut(s) 360, 440, 463, 547
BstDEI CTNAG 3 cut(s) 387, 500, 594
BstFNI CGCG 2 cut(s) 143, 197
BstHHI GCGC 4 cut(s) 143, 199, 545, 744
BstKTI GATC 4 cut(s) 73, 244, 274, 320
BstMAI GTCTC 1 cut(s) 590
BstMBI GATC 4 cut(s) 70, 241, 271, 317
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 3 cut(s) 235, 448, 788
BstNI CCWGG 2 cut(s) 307, 819
BstNSI RCATGY 1 cut(s) 717
BstSCI CCNGG 3 cut(s) 305, 817, 849
BstSFI CTRYAG 1 cut(s) 227
BstUI CGCG 2 cut(s) 143, 197
BstV1I GCAGC 3 cut(s) 162, 370, 766
BstZ17I GTATAC 1 cut(s) 752
Bsu15I ATCGAT 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 500
BsuRI GGCC 2 cut(s) 293, 791
BsuTUI ATCGAT 1 cut(s) 316
BtgZI GCGATG 1 cut(s) 178
Cac8I GCNNGC 4 cut(s) 360, 440, 463, 547
CfoI GCGC 4 cut(s) 143, 199, 545, 744
Cfr10I RCCGGY 2 cut(s) 137, 791
Cfr13I GGNCC 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 316
Csp6I GTAC 2 cut(s) 581, 633
CviAII CATG 4 cut(s) 466, 714, 845, 905
CviQI GTAC 2 cut(s) 581, 633
DdeI CTNAG 3 cut(s) 387, 500, 594
DpnI GATC 4 cut(s) 72, 243, 273, 319
DpnII GATC 4 cut(s) 70, 241, 271, 317
DrdI GACNNNNNNGTC 1 cut(s) 275
DriI GACNNNNNGTC 1 cut(s) 208
DseDI GACNNNNNNGTC 1 cut(s) 275
Eam1105I GACNNNNNGTC 1 cut(s) 208
EciI GGCGGA 2 cut(s) 274, 520
Ecl136II GAGCTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 288
Eco147I AGGCCT 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 150
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 1 cut(s) 277
Eco53kI GAGCTC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 605
Eco81I CCTNAGG 1 cut(s) 500
Eco88I CYCGRG 2 cut(s) 149, 299
EcoICRI GAGCTC 1 cut(s) 148
EcoRI GAATTC 1 cut(s) 809
EcoRII CCWGG 2 cut(s) 305, 817
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 288
EcoT22I ATGCAT 1 cut(s) 906
EcoT38I GRGCYC 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 4 cut(s) 469, 717, 848, 908
FaqI GGGAC 1 cut(s) 59
FatI CATG 4 cut(s) 465, 713, 844, 904
FblI GTMKAC 1 cut(s) 751
Fnu4HI GCNGC 4 cut(s) 176, 384, 477, 780
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 4 cut(s) 176, 384, 477, 780
GlaI GCGC 4 cut(s) 142, 198, 544, 743
GluI GCNGC 4 cut(s) 176, 384, 477, 780
GsuI CTGGAG 4 cut(s) 438, 539, 597, 840
HaeIII GGCC 2 cut(s) 293, 791
HapII CCGG 3 cut(s) 138, 792, 851
HhaI GCGC 4 cut(s) 143, 199, 545, 744
Hin1II CATG 4 cut(s) 469, 717, 848, 908
Hin6I GCGC 4 cut(s) 141, 197, 543, 742
HinP1I GCGC 4 cut(s) 141, 197, 543, 742
HincII GTYRAC 1 cut(s) 515
HindII GTYRAC 1 cut(s) 515
HinfI GANTC 5 cut(s) 170, 302, 425, 508, 569
HpaII CCGG 3 cut(s) 138, 792, 851
HphI GGTGA 2 cut(s) 43, 694
Hpy166II GTNNAC 4 cut(s) 429, 515, 711, 752
Hpy188I TCNGA 1 cut(s) 390
Hpy188III TCNNGA 4 cut(s) 88, 527, 556, 881
Hpy8I GTNNAC 4 cut(s) 429, 515, 711, 752
Hpy99I CGWCG 1 cut(s) 272
HpyAV CCTTC 6 cut(s) 22, 31, 117, 154, 181, 763
HpyCH4III ACNGT 1 cut(s) 836
HpyCH4IV ACGT 1 cut(s) 758
HpyCH4V TGCA 6 cut(s) 229, 358, 451, 658, 763, 904
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 448, 788
HpyF3I CTNAG 3 cut(s) 387, 500, 594
HpySE526I ACGT 1 cut(s) 758
Hsp92II CATG 4 cut(s) 469, 717, 848, 908
HspAI GCGC 4 cut(s) 141, 197, 543, 742
Kzo9I GATC 4 cut(s) 70, 241, 271, 317
LmnI GCTCC 6 cut(s) 13, 145, 256, 413, 540, 821
Lsp1109I GCAGC 3 cut(s) 162, 370, 766
LweI GCATC 2 cut(s) 201, 495
MaeII ACGT 1 cut(s) 758
MaeIII GTNAC 1 cut(s) 772
MalI GATC 4 cut(s) 72, 243, 273, 319
MboI GATC 4 cut(s) 70, 241, 271, 317
MfeI CAATTG 1 cut(s) 257
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 5 cut(s) 220, 257, 352, 687, 809
MlyI GAGTC 4 cut(s) 164, 311, 434, 502
MnlI CCTC 9 cut(s) 13, 264, 324, 336, 390, 413, 523, 559, 759
Mph1103I ATGCAT 1 cut(s) 906
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 2 cut(s) 626, 899
MspA1I CMGCKG 1 cut(s) 779
MspI CCGG 3 cut(s) 138, 792, 851
MspR9I CCNGG 3 cut(s) 307, 819, 851
MunI CAATTG 1 cut(s) 257
Mva1269I GAATGC 1 cut(s) 352
MvaI CCWGG 2 cut(s) 307, 819
MvnI CGCG 2 cut(s) 143, 197
MwoI GCNNNNNNNGC 3 cut(s) 235, 448, 788
NciI CCSGG 1 cut(s) 851
NdeII GATC 4 cut(s) 70, 241, 271, 317
NlaIII CATG 4 cut(s) 469, 717, 848, 908
NlaIV GGNNCC 1 cut(s) 885
NsiI ATGCAT 1 cut(s) 906
NspI RCATGY 1 cut(s) 717
NspV TTCGAA 1 cut(s) 737
PaeR7I CTCGAG 2 cut(s) 149, 299
PceI AGGCCT 1 cut(s) 293
PciI ACATGT 1 cut(s) 713
PctI GAATGC 1 cut(s) 352
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 1 cut(s) 569
PfoI TCCNGGA 1 cut(s) 305
PkrI GCNGC 4 cut(s) 177, 385, 478, 781
PleI GAGTC 4 cut(s) 164, 310, 433, 502
PpsI GAGTC 4 cut(s) 164, 310, 433, 502
PscI ACATGT 1 cut(s) 713
PshBI ATTAAT 1 cut(s) 899
Psp124BI GAGCTC 1 cut(s) 150
Psp6I CCWGG 2 cut(s) 305, 817
PspGI CCWGG 2 cut(s) 305, 817
PspN4I GGNNCC 1 cut(s) 885
PspPI GGNCC 1 cut(s) 277
PspXI VCTCGAGB 2 cut(s) 149, 299
PstI CTGCAG 1 cut(s) 231
PvuII CAGCTG 1 cut(s) 779
RsaI GTAC 2 cut(s) 582, 634
RsaNI GTAC 2 cut(s) 581, 633
SacI GAGCTC 1 cut(s) 150
SaqAI TTAA 2 cut(s) 626, 899
SatI GCNGC 4 cut(s) 176, 384, 477, 780
Sau3AI GATC 4 cut(s) 70, 241, 271, 317
Sau96I GGNCC 1 cut(s) 277
ScaI AGTACT 1 cut(s) 582
SchI GAGTC 4 cut(s) 164, 311, 434, 502
ScrFI CCNGG 3 cut(s) 307, 819, 851
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 2 cut(s) 201, 495
SfcI CTRYAG 1 cut(s) 227
Sfr274I CTCGAG 2 cut(s) 149, 299
SfuI TTCGAA 1 cut(s) 737
SinI GGWCC 1 cut(s) 277
SlaI CTCGAG 2 cut(s) 149, 299
SmlI CTYRAG 2 cut(s) 149, 299
SmoI CTYRAG 2 cut(s) 149, 299
Sse9I AATT 5 cut(s) 220, 257, 352, 687, 809
SseBI AGGCCT 1 cut(s) 293
SsiI CCGC 4 cut(s) 143, 285, 477, 505
SspI AATATT 1 cut(s) 373
SstI GAGCTC 1 cut(s) 150
StuI AGGCCT 1 cut(s) 293
StyD4I CCNGG 3 cut(s) 305, 817, 849
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 1 cut(s) 836
TaiI ACGT 1 cut(s) 761
TaqI TCGA 7 cut(s) 87, 150, 240, 300, 316, 564, 737
TaqII GACCGA 1 cut(s) 281
TasI AATT 5 cut(s) 220, 257, 352, 687, 809
TatI WGTACW 1 cut(s) 580
TauI GCSGC 1 cut(s) 479
TfiI GAWTC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 626, 899
Tru9I TTAA 2 cut(s) 626, 899
TseI GCWGC 3 cut(s) 175, 383, 779
TspDTI ATGAA 1 cut(s) 860
VpaK11BI GGWCC 1 cut(s) 277
VspI ATTAAT 1 cut(s) 899
XapI RAATTY 2 cut(s) 352, 809
XceI RCATGY 1 cut(s) 717
XhoI CTCGAG 2 cut(s) 149, 299
XmiI GTMKAC 1 cut(s) 751
XmnI GAANNNNTTC 1 cut(s) 809
ZrmI AGTACT 1 cut(s) 582
Zsp2I ATGCAT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.