MD07G1006700.v1.1

DNA-damage-repair toleration protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0012848
Physical Location & Seq
Forward (+)
640738 .. 641872
1135 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1006700.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGAAGATGATGAGGTTGCTCTTAATCTCAGTGGCAACATTCTGTGCCATTGTCTCTACTGTGAGCTCTTCTTGTTCCCCATCAGACCTAGCAGCACTTCAATCCATCAGAACCGGACTCACCGATTCCTCATTGGGCATCTTCAACTCCTGGGTCGGAACCGATTGCTGCGTAAACTGGTACGGAGTCAGCTGCGATCCCACAACCGGCCGAGTCGTCGACATTAATCTCCGAGGCGAATCGGAGGACCCCATACTCAAGAAATCCGGTCAGTCCGGGTTCATGTCAGGATCTATCTCGCCCAAAATTTGCAGCCTCGACCGCCTCACTACCCTCGTTCTCGCTGACTGGAAGGGGGTCACCGGTGAGATCCCCCAATGCCTCACCACCCTCTCCAACCTCCGAGTTCTCGATCTCGTCGGAAACAAGATTTCGGGCAAGATTCCGGCCGATATCGGCAACCTCAAGATGCTCAGAGTCCTCAACCTCGCCGATAACCAGATCTCCGGTAAGATTCCGGCGAGTCTCGTTGGCCTCTCCGGGCTAATGCACATGGACCTCAGCAACAACCAAATCACGGGCGAGTTACCGGCCGATTTCGGAAAACTCAAGATGCTGAGCCGGGCTCTGCTGAACCGAAACCAGCTCACCGGGTCGATCCCCGATTCCATTGGCAACATGAACCGGTTGGCCGACCTGGACCTGTCCAGGAACCAAATGTGGGGTTCCGTACCGGACTGTCTTGGGAAAATGCAGGTTCTTTCGACGTTGAATTTGGACGGAAACAAGTTTTCGGGTCAATTGCCAGCGTCCGTTTTGAGCAATCGGGGTATGGGGATCTTAAATTTGAGCCGAAATGGGTTCGAAGGTAACATACCGGACGTTTTCCACGGAAATTCGTACTTCATGGCACTGGATTTATCGTACAACAATTTAAAGGGCCCGATACCCGGTTCATTATCGGCGGCGAAATATATCGGGCACTTGGATCTCAGCCACAACCACCTGTGCGGGGCGATTCCTGTGGGGAACCCGTTCGATCACCTGGAAGTGTCGTCGTTTACCAACAATGATTGCCTCTGCGGGAACCCGCTGAGTACTTGTTGA

Protein Analysis

369

Amino Acids

39.26

Weight (kDa)

6.58

Isoelectric Point (pI)

28.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 8.1e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 101 - 207 2.4e-11 Leucine-rich repeat region
LRR_8 PF13855 109 - 168 1.5e-06 Leucine rich repeat
LRR_8 PF13855 211 - 264 8.8e-06 Leucine rich repeat
LRR_8 PF13855 280 - 336 3.8e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12610
fragaria_vesca FvH4_7g00720
malus_domestica MD02G1315200.v1.1 MD07G1006700.v1.1
prunus_persica Prupe.2G009800_v2.0.a1
pyrus_communis pycom02g26420 pycom07g00540
rosa_chinensis RchiOBHm_Chr1g0314931
rosa_laevigata RLG00000030754
rosa_multiflora Rmu_co8044478.1_g000001 Rmu_sc0005015.1_g000002 Rmu_sc0005168.1_g000009
rosa_roxburghii Rroxscaffold_4G00331670
rosa_rugosa Rorug01G0003100
rosa_samantha Rh1AG014000 Rh1BG005600 Rh1DG007600
rosa_wichuraiana Rw1G000660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 745
AccI GTMKAC 1 cut(s) 219
AciI CCGC 5 cut(s) 322, 965, 1011, 1083, 1091
AclWI GGATC 6 cut(s) 191, 298, 364, 652, 845, 996
AcoI YGGCCR 4 cut(s) 208, 447, 591, 690
AcsI RAATTY 4 cut(s) 306, 772, 844, 895
AfaI GTAC 5 cut(s) 182, 732, 902, 926, 1099
AfiI CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
AgeI ACCGGT 2 cut(s) 362, 684
AgsI TTSAA 3 cut(s) 101, 145, 772
AjnI CCWGG 4 cut(s) 149, 696, 707, 1044
AluBI AGCT 3 cut(s) 66, 192, 646
AluI AGCT 3 cut(s) 66, 192, 646
Alw21I GWGCWC 1 cut(s) 68
Alw26I GTCTC 2 cut(s) 58, 530
AlwI GGATC 6 cut(s) 191, 298, 364, 652, 845, 996
AoxI GGCC 6 cut(s) 208, 447, 532, 591, 690, 940
ApaI GGGCCC 1 cut(s) 944
ApeKI GCWGC 4 cut(s) 92, 168, 192, 312
ApoI RAATTY 4 cut(s) 306, 772, 844, 895
ArsI GACNNNNNNTTYG 2 cut(s) 757, 789
AseI ATTAAT 1 cut(s) 225
AsiGI ACCGGT 2 cut(s) 362, 684
Asp700I GAANNNNTTC 1 cut(s) 1034
AspS9I GGNCC 5 cut(s) 247, 556, 700, 940, 941
AsuC2I CCSGG 5 cut(s) 277, 541, 623, 652, 951
AsuHPI GGTGA 6 cut(s) 112, 352, 376, 377, 640, 1034
AsuII TTCGAA 1 cut(s) 864
AvaII GGWCC 3 cut(s) 247, 556, 700
BaeGI GKGCMC 2 cut(s) 944, 984
BanII GRGCYC 3 cut(s) 68, 628, 944
BarI GAAGNNNNNNTAC 2 cut(s) 858, 890
Bbv12I GWGCWC 1 cut(s) 68
BbvCI CCTCAGC 1 cut(s) 560
BbvI GCAGC 4 cut(s) 104, 155, 179, 324
BccI CCATC 2 cut(s) 88, 113
BciT130I CCWGG 4 cut(s) 151, 698, 709, 1046
BcnI CCSGG 5 cut(s) 277, 541, 623, 652, 951
BcoDI GTCTC 2 cut(s) 58, 530
BfaI CTAG 1 cut(s) 89
BfuAI ACCTGC 1 cut(s) 745
BglII AGATCT 1 cut(s) 501
BisI GCNGC 5 cut(s) 93, 169, 193, 313, 966
BlpI GCTNAGC 1 cut(s) 617
BlsI GCNGC 5 cut(s) 94, 170, 194, 314, 967
BmcAI AGTACT 1 cut(s) 1099
Bme1390I CCNGG 9 cut(s) 151, 277, 541, 623, 652, 698, 709, 951, 1046
Bme18I GGWCC 3 cut(s) 247, 556, 700
BmgT120I GGNCC 5 cut(s) 247, 556, 700, 940, 941
BmiI GGNNCC 7 cut(s) 160, 249, 713, 727, 942, 1031, 1088
BmrFI CCNGG 9 cut(s) 151, 277, 541, 623, 652, 698, 709, 951, 1046
BmsI GCATC 3 cut(s) 147, 459, 603
BplI GAGNNNNNCTC 2 cut(s) 610, 642
Bpu10I CCTNAGC 1 cut(s) 560
Bpu1102I GCTNAGC 1 cut(s) 617
Bpu14I TTCGAA 1 cut(s) 864
BpuEI CTTGAG 3 cut(s) 242, 449, 593
BpuMI CCSGG 5 cut(s) 277, 541, 623, 652, 951
BsaJI CCNNGG 3 cut(s) 150, 232, 889
BsaWI WCCGGW 7 cut(s) 113, 266, 362, 506, 684, 733, 877
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
Bse118I RCCGGY 4 cut(s) 206, 362, 589, 684
Bse1I ACTGG 3 cut(s) 182, 353, 918
BseBI CCWGG 4 cut(s) 151, 698, 709, 1046
BseDI CCNNGG 3 cut(s) 150, 232, 889
BseLI CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
BseMII CTCAG 6 cut(s) 42, 487, 574, 608, 1006, 1085
BseNI ACTGG 3 cut(s) 182, 353, 918
BseSI GKGCMC 2 cut(s) 944, 984
BseX3I CGGCCG 3 cut(s) 208, 447, 591
BseXI GCAGC 4 cut(s) 104, 155, 179, 324
Bsh1285I CGRYCG 4 cut(s) 211, 322, 450, 594
BshFI GGCC 6 cut(s) 210, 449, 534, 593, 692, 942
BshTI ACCGGT 2 cut(s) 362, 684
BsiEI CGRYCG 4 cut(s) 211, 322, 450, 594
BsiHKAI GWGCWC 1 cut(s) 68
BslI CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
BsmAI GTCTC 2 cut(s) 58, 530
BsnI GGCC 6 cut(s) 210, 449, 534, 593, 692, 942
Bsp119I TTCGAA 1 cut(s) 864
Bsp120I GGGCCC 1 cut(s) 940
Bsp1286I GDGCHC 4 cut(s) 68, 628, 944, 984
Bsp143I GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
Bsp1720I GCTNAGC 1 cut(s) 617
BspACI CCGC 5 cut(s) 322, 965, 1011, 1083, 1091
BspANI GGCC 6 cut(s) 210, 449, 534, 593, 692, 942
BspCNI CTCAG 6 cut(s) 41, 486, 573, 609, 1005, 1086
BspLI GGNNCC 7 cut(s) 160, 249, 713, 727, 942, 1031, 1088
BspMI ACCTGC 1 cut(s) 745
BspPI GGATC 6 cut(s) 191, 298, 364, 652, 845, 996
BspQI GCTCTTC 1 cut(s) 73
BspT104I TTCGAA 1 cut(s) 864
BsrFI RCCGGY 4 cut(s) 206, 362, 589, 684
BsrI ACTGG 3 cut(s) 182, 353, 918
BssAI RCCGGY 4 cut(s) 206, 362, 589, 684
BssECI CCNNGG 3 cut(s) 150, 232, 889
BssMI GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
Bst2UI CCWGG 4 cut(s) 151, 698, 709, 1046
Bst4CI ACNGT 2 cut(s) 61, 740
Bst6I CTCTTC 1 cut(s) 73
BstBI TTCGAA 1 cut(s) 864
BstC8I GCNNGC 1 cut(s) 807
BstDEI CTNAG 6 cut(s) 28, 473, 560, 617, 992, 1094
BstDSI CCRYGG 1 cut(s) 889
BstEII GGTNACC 1 cut(s) 358
BstKTI GATC 9 cut(s) 199, 293, 372, 415, 504, 660, 840, 991, 1042
BstMAI GTCTC 2 cut(s) 58, 530
BstMBI GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
BstMCI CGRYCG 4 cut(s) 211, 322, 450, 594
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstNI CCWGG 4 cut(s) 151, 698, 709, 1046
BstPI GGTNACC 1 cut(s) 358
BstSCI CCNGG 9 cut(s) 149, 275, 539, 621, 650, 696, 707, 949, 1044
BstSLI GKGCMC 2 cut(s) 944, 984
BstV1I GCAGC 4 cut(s) 104, 155, 179, 324
BstX2I RGATCY 5 cut(s) 290, 369, 501, 837, 988
BstYI RGATCY 5 cut(s) 290, 369, 501, 837, 988
BstZI CGGCCG 3 cut(s) 208, 447, 591
BsuRI GGCC 6 cut(s) 210, 449, 534, 593, 692, 942
BtgI CCRYGG 1 cut(s) 889
BtsIMutI CAGTG 2 cut(s) 36, 911
BveI ACCTGC 1 cut(s) 745
Cac8I GCNNGC 1 cut(s) 807
Cfr10I RCCGGY 4 cut(s) 206, 362, 589, 684
Cfr13I GGNCC 5 cut(s) 247, 556, 700, 940, 941
CseI GACGC 1 cut(s) 798
Csp6I GTAC 5 cut(s) 181, 731, 901, 925, 1098
CspAI ACCGGT 2 cut(s) 362, 684
CviAII CATG 4 cut(s) 283, 553, 679, 907
CviQI GTAC 5 cut(s) 181, 731, 901, 925, 1098
DdeI CTNAG 6 cut(s) 28, 473, 560, 617, 992, 1094
DpnI GATC 9 cut(s) 198, 292, 371, 414, 503, 659, 839, 990, 1041
DpnII GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
DraI TTTAAA 1 cut(s) 936
EaeI YGGCCR 4 cut(s) 208, 447, 591, 690
EagI CGGCCG 3 cut(s) 208, 447, 591
Eam1104I CTCTTC 1 cut(s) 73
EarI CTCTTC 1 cut(s) 73
Ecl136II GAGCTC 1 cut(s) 66
EclXI CGGCCG 3 cut(s) 208, 447, 591
Eco24I GRGCYC 3 cut(s) 68, 628, 944
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 3 cut(s) 247, 556, 700
Eco52I CGGCCG 3 cut(s) 208, 447, 591
Eco53kI GAGCTC 1 cut(s) 66
Eco91I GGTNACC 1 cut(s) 358
EcoICRI GAGCTC 1 cut(s) 66
EcoO109I RGGNCCY 2 cut(s) 247, 940
EcoO65I GGTNACC 1 cut(s) 358
EcoRII CCWGG 4 cut(s) 149, 696, 707, 1044
EcoRV GATATC 1 cut(s) 454
EcoT38I GRGCYC 3 cut(s) 68, 628, 944
FaeI CATG 4 cut(s) 286, 556, 682, 910
FaiI YATR 8 cut(s) 254, 284, 554, 680, 833, 875, 908, 975
FatI CATG 4 cut(s) 282, 552, 678, 906
FauI CCCGC 3 cut(s) 1004, 1076, 1098
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 5 cut(s) 93, 169, 193, 313, 966
FriOI GRGCYC 3 cut(s) 68, 628, 944
Fsp4HI GCNGC 5 cut(s) 93, 169, 193, 313, 966
FspBI CTAG 1 cut(s) 89
GluI GCNGC 5 cut(s) 93, 169, 193, 313, 966
HaeIII GGCC 6 cut(s) 210, 449, 534, 593, 692, 942
HgaI GACGC 1 cut(s) 798
Hin1II CATG 4 cut(s) 286, 556, 682, 910
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HphI GGTGA 6 cut(s) 112, 352, 376, 377, 640, 1034
Hpy166II GTNNAC 3 cut(s) 175, 220, 1062
Hpy188I TCNGA 9 cut(s) 85, 110, 158, 233, 244, 404, 422, 476, 602
Hpy188III TCNNGA 5 cut(s) 259, 288, 410, 466, 610
Hpy8I GTNNAC 3 cut(s) 175, 220, 1062
Hpy99I CGWCG 4 cut(s) 221, 422, 769, 1060
HpyAV CCTTC 2 cut(s) 346, 860
HpyCH4III ACNGT 2 cut(s) 61, 740
HpyCH4IV ACGT 2 cut(s) 767, 882
HpyCH4V TGCA 3 cut(s) 312, 550, 754
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 6 cut(s) 28, 473, 560, 617, 992, 1094
HpySE526I ACGT 2 cut(s) 767, 882
Hsp92II CATG 4 cut(s) 286, 556, 682, 910
Kzo9I GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
LguI GCTCTTC 1 cut(s) 73
Lsp1109I GCAGC 4 cut(s) 104, 155, 179, 324
LweI GCATC 3 cut(s) 147, 459, 603
MaeI CTAG 1 cut(s) 89
MaeII ACGT 2 cut(s) 767, 882
MaeIII GTNAC 3 cut(s) 358, 585, 869
MalI GATC 9 cut(s) 198, 292, 371, 414, 503, 659, 839, 990, 1041
MboI GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
MboII GAAGA 3 cut(s) 16, 60, 133
MfeI CAATTG 1 cut(s) 800
MflI RGATCY 5 cut(s) 290, 369, 501, 837, 988
MhlI GDGCHC 4 cut(s) 68, 628, 944, 984
MluCI AATT 6 cut(s) 306, 772, 800, 844, 895, 931
MlyI GAGTC 5 cut(s) 111, 195, 222, 486, 532
MmeI TCCRAC 3 cut(s) 136, 400, 420
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 4 cut(s) 23, 225, 842, 935
MspA1I CMGCKG 2 cut(s) 192, 1093
MspR9I CCNGG 9 cut(s) 151, 277, 541, 623, 652, 698, 709, 951, 1046
MunI CAATTG 1 cut(s) 800
MvaI CCWGG 4 cut(s) 151, 698, 709, 1046
MwoI GCNNNNNNNGC 1 cut(s) 321
NciI CCSGG 5 cut(s) 277, 541, 623, 652, 951
NdeII GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
NlaIII CATG 4 cut(s) 286, 556, 682, 910
NlaIV GGNNCC 7 cut(s) 160, 249, 713, 727, 942, 1031, 1088
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 1 cut(s) 358
NspV TTCGAA 1 cut(s) 864
PciSI GCTCTTC 1 cut(s) 73
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 6 cut(s) 125, 239, 442, 514, 665, 1018
PfoI TCCNGGA 1 cut(s) 707
PinAI ACCGGT 2 cut(s) 362, 684
PkrI GCNGC 5 cut(s) 94, 170, 194, 314, 967
PleI GAGTC 5 cut(s) 111, 194, 221, 485, 531
PpsI GAGTC 5 cut(s) 111, 194, 221, 485, 531
PpuMI RGGWCCY 1 cut(s) 247
PshBI ATTAAT 1 cut(s) 225
Psp124BI GAGCTC 1 cut(s) 68
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 4 cut(s) 149, 696, 707, 1044
PspEI GGTNACC 1 cut(s) 358
PspGI CCWGG 4 cut(s) 149, 696, 707, 1044
PspN4I GGNNCC 7 cut(s) 160, 249, 713, 727, 942, 1031, 1088
PspOMI GGGCCC 1 cut(s) 940
PspPI GGNCC 5 cut(s) 247, 556, 700, 940, 941
PspPPI RGGWCCY 1 cut(s) 247
PsuI RGATCY 5 cut(s) 290, 369, 501, 837, 988
PvuII CAGCTG 1 cut(s) 192
RsaI GTAC 5 cut(s) 182, 732, 902, 926, 1099
RsaNI GTAC 5 cut(s) 181, 731, 901, 925, 1098
SacI GAGCTC 1 cut(s) 68
SalI GTCGAC 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 73
SaqAI TTAA 4 cut(s) 23, 225, 842, 935
SatI GCNGC 5 cut(s) 93, 169, 193, 313, 966
Sau3AI GATC 9 cut(s) 196, 290, 369, 412, 501, 657, 837, 988, 1039
Sau96I GGNCC 5 cut(s) 247, 556, 700, 940, 941
ScaI AGTACT 1 cut(s) 1099
SchI GAGTC 5 cut(s) 111, 195, 222, 486, 532
ScrFI CCNGG 9 cut(s) 151, 277, 541, 623, 652, 698, 709, 951, 1046
SduI GDGCHC 4 cut(s) 68, 628, 944, 984
SfaNI GCATC 3 cut(s) 147, 459, 603
SfuI TTCGAA 1 cut(s) 864
SgrAI CRCCGGYG 1 cut(s) 362
SinI GGWCC 3 cut(s) 247, 556, 700
SmlI CTYRAG 3 cut(s) 257, 464, 608
SmoI CTYRAG 3 cut(s) 257, 464, 608
Sse9I AATT 6 cut(s) 306, 772, 800, 844, 895, 931
SsiI CCGC 5 cut(s) 322, 965, 1011, 1083, 1091
SspMI CTAG 1 cut(s) 89
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 9 cut(s) 149, 275, 539, 621, 650, 696, 707, 949, 1044
TaaI ACNGT 2 cut(s) 61, 740
TaiI ACGT 2 cut(s) 770, 885
TaqI TCGA 7 cut(s) 219, 318, 411, 656, 764, 864, 1038
TasI AATT 6 cut(s) 306, 772, 800, 844, 895, 931
TatI WGTACW 1 cut(s) 1097
TauI GCSGC 1 cut(s) 968
TfiI GAWTC 6 cut(s) 125, 239, 442, 514, 665, 1018
Tru1I TTAA 4 cut(s) 23, 225, 842, 935
Tru9I TTAA 4 cut(s) 23, 225, 842, 935
TscAI CASTG 2 cut(s) 36, 918
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 4 cut(s) 92, 168, 192, 312
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 5 cut(s) 17, 271, 695, 895, 945
TspGWI ACGGA 5 cut(s) 198, 718, 795, 802, 906
TspRI CASTG 2 cut(s) 36, 918
VpaK11BI GGWCC 3 cut(s) 247, 556, 700
VspI ATTAAT 1 cut(s) 225
XapI RAATTY 4 cut(s) 306, 772, 844, 895
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 1 cut(s) 89
ZrmI AGTACT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.