pycom02g26420

DNA-damage-repair toleration protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012848
Physical Location & Seq
Reverse (-)
24207548 .. 24208654
1107 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g26420.1

Sequence Viewer

Length: 1107 bp
ATGAAGATGGTGAGGTTGCTCTTAATCTCATTGGCAACATTCTGTGCCATTGTCTCAACAGTGAGCTCCTCTTGTTCGTCATCAGACCTAGCAGCACTTCAATCCATCAAAACCGGCCTCACCGATTCCTCCTTGGGCATCTTCAACTCCTGGGTCGGAACAGATTGCTGCGTAAACTGGTACGGAGTCAGCTGCGATCCTACAACCGGCCGAGTCGTCGACATTAATCTCCGAGGCGAATCGGAAGACCCGATACTCATGAAATCAGGCCAGTCCGGGTTTATGTCCGGCTCGATCTCACCCCAAATTTGCAGCCTCGACCGTCTCACCACCCTCGTTCTAGCCGACTGGAAGGGAGTCACCGGCAAGATTCCCCAATGCCTCGCCACCCTCTCCAATCTCCGAATCCTCGACCTTGTTGGAAACAAGATTTCGGGCGGGATTCCGGCCGATATCGGCAACCTCAAGATGCTTACGGTCCTCAACCTCGCCGATAACCAGATCTCCGGTAAGATTCCGGCGAGTCTCGTGAGCCTCTCAGGGCTAATGCACCTGGACCTCAGCAACAACCAAATCTCGGGCGAGATACCGGCCGATTTCGGAAAACTCAAGATGCTCAGCCGGGCTCTGCTGTACCGGAACCAGCTCACCGGGTCAATCCCGGACTCCATCGGCAACATGAACCGGTTGGCCGACCTGGACCTGTCCAGGAACCAATTATCGGGTCGCGTACCGGATTGTCTCGGAAAAATGCGGGTTCTTTCGACGTTGAATCTGGACGGAAACTCGTTTTCTGGTCAATTACCAGCGTCCCTTTTGAGCAATCGGGGTTTGGGGATCTTGAATATGAGCCGAAACGGGTTTGAAGGTAACATACCGGACGTTTTCCACGGAAATTCGTACTTTATGGTGCTCGATTTGTCGTACAACAAATTGAAGGGTCCGATTCCCGGTTCGTTATCGTCGGCGAAATATATCGGGCACTTGGATCTGAGCCACAACCACCTGTGCGGGGCGATTCCTGTGGGGAACCCGTTCGACCACCTCGGAGCATCGTCGTTTGCCAACAATGATTGCCTCTGCGGGAACCCGCTGAGTACCTGTTGA

Protein Analysis

369

Amino Acids

39.0

Weight (kDa)

6.96

Isoelectric Point (pI)

30.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 66 5.1e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 101 - 278 4.9e-15 Leucine-rich repeat region
LRR_8 PF13855 158 - 216 2.1e-06 Leucine rich repeat
LRR_8 PF13855 212 - 263 1.9e-06 Leucine rich repeat
LRR_8 PF13855 277 - 336 4.1e-07 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12610
fragaria_vesca FvH4_7g00720
malus_domestica MD02G1315200.v1.1 MD07G1006700.v1.1
prunus_persica Prupe.2G009800_v2.0.a1
pyrus_communis pycom02g26420 pycom07g00540
rosa_chinensis RchiOBHm_Chr1g0314931
rosa_laevigata RLG00000030754
rosa_multiflora Rmu_co8044478.1_g000001 Rmu_sc0005015.1_g000002 Rmu_sc0005168.1_g000009
rosa_roxburghii Rroxscaffold_4G00331670
rosa_rugosa Rorug01G0003100
rosa_samantha Rh1AG014000 Rh1BG005600 Rh1DG007600
rosa_wichuraiana Rw1G000660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 219
AccII CGCG 1 cut(s) 729
AciI CCGC 5 cut(s) 438, 754, 1011, 1083, 1091
AclWI GGATC 3 cut(s) 191, 845, 996
AcoI YGGCCR 4 cut(s) 208, 447, 591, 690
AcsI RAATTY 2 cut(s) 306, 895
AfaI GTAC 6 cut(s) 182, 635, 732, 902, 926, 1099
AfiI CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
AgeI ACCGGT 1 cut(s) 684
AgsI TTSAA 6 cut(s) 101, 145, 772, 844, 866, 937
AjnI CCWGG 4 cut(s) 149, 552, 696, 707
AluBI AGCT 3 cut(s) 66, 192, 646
AluI AGCT 3 cut(s) 66, 192, 646
Alw21I GWGCWC 2 cut(s) 68, 915
Alw26I GTCTC 4 cut(s) 58, 329, 530, 746
AlwI GGATC 3 cut(s) 191, 845, 996
Ama87I CYCGRG 1 cut(s) 577
AoxI GGCC 6 cut(s) 115, 208, 268, 447, 591, 690
ApeKI GCWGC 4 cut(s) 92, 168, 192, 312
ApoI RAATTY 2 cut(s) 306, 895
AseI ATTAAT 1 cut(s) 225
AsiGI ACCGGT 1 cut(s) 684
Asp700I GAANNNNTTC 1 cut(s) 1034
AspS9I GGNCC 4 cut(s) 478, 556, 700, 941
AsuC2I CCSGG 5 cut(s) 277, 623, 652, 662, 951
AsuHPI GGTGA 6 cut(s) 22, 112, 291, 319, 352, 640
AvaI CYCGRG 1 cut(s) 577
AvaII GGWCC 4 cut(s) 478, 556, 700, 941
BaeGI GKGCMC 1 cut(s) 984
BanII GRGCYC 2 cut(s) 68, 628
BarI GAAGNNNNNNTAC 4 cut(s) 237, 269, 858, 890
BauI CACGAG 1 cut(s) 527
BbsI GAAGAC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 68, 915
BbvCI CCTCAGC 1 cut(s) 560
BbvI GCAGC 4 cut(s) 104, 155, 179, 324
BccI CCATC 2 cut(s) 113, 677
BciT130I CCWGG 4 cut(s) 151, 554, 698, 709
BcnI CCSGG 5 cut(s) 277, 623, 652, 662, 951
BcoDI GTCTC 4 cut(s) 58, 329, 530, 746
BfaI CTAG 2 cut(s) 89, 341
BglII AGATCT 1 cut(s) 501
BisI GCNGC 4 cut(s) 93, 169, 193, 313
BlpI GCTNAGC 1 cut(s) 617
BlsI GCNGC 4 cut(s) 94, 170, 194, 314
Bme1390I CCNGG 9 cut(s) 151, 277, 554, 623, 652, 662, 698, 709, 951
Bme18I GGWCC 4 cut(s) 478, 556, 700, 941
BmeT110I CYCGRG 1 cut(s) 577
BmgT120I GGNCC 4 cut(s) 478, 556, 700, 941
BmiI GGNNCC 5 cut(s) 641, 713, 942, 1031, 1088
BmrFI CCNGG 9 cut(s) 151, 277, 554, 623, 652, 662, 698, 709, 951
BmsI GCATC 4 cut(s) 147, 459, 603, 1061
BpiI GAAGAC 1 cut(s) 252
Bpu10I CCTNAGC 1 cut(s) 560
Bpu1102I GCTNAGC 1 cut(s) 617
BpuEI CTTGAG 2 cut(s) 449, 593
BpuMI CCSGG 5 cut(s) 277, 623, 652, 662, 951
BsaJI CCNNGG 5 cut(s) 132, 150, 232, 889, 1045
BsaWI WCCGGW 5 cut(s) 506, 636, 684, 733, 877
BsaXI ACNNNNNCTCC 2 cut(s) 488, 518
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
Bse118I RCCGGY 5 cut(s) 113, 206, 362, 589, 684
Bse1I ACTGG 3 cut(s) 182, 271, 353
BseBI CCWGG 4 cut(s) 151, 554, 698, 709
BseDI CCNNGG 5 cut(s) 132, 150, 232, 889, 1045
BseLI CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
BseMII CTCAG 5 cut(s) 552, 574, 631, 983, 1085
BseNI ACTGG 3 cut(s) 182, 271, 353
BseRI GAGGAG 1 cut(s) 58
BseSI GKGCMC 1 cut(s) 984
BseX3I CGGCCG 3 cut(s) 208, 447, 591
BseXI GCAGC 4 cut(s) 104, 155, 179, 324
Bsh1236I CGCG 1 cut(s) 729
Bsh1285I CGRYCG 4 cut(s) 211, 322, 450, 594
BshFI GGCC 6 cut(s) 117, 210, 270, 449, 593, 692
BshTI ACCGGT 1 cut(s) 684
BsiEI CGRYCG 4 cut(s) 211, 322, 450, 594
BsiHKAI GWGCWC 2 cut(s) 68, 915
BsiHKCI CYCGRG 1 cut(s) 577
BslFI GGGAC 1 cut(s) 796
BslI CCNNNNNNNGG 5 cut(s) 206, 577, 721, 950, 1012
BsmAI GTCTC 4 cut(s) 58, 329, 530, 746
BsmBI CGTCTC 1 cut(s) 329
BsmFI GGGAC 1 cut(s) 796
BsnI GGCC 6 cut(s) 117, 210, 270, 449, 593, 692
BsoBI CYCGRG 1 cut(s) 577
Bsp1286I GDGCHC 4 cut(s) 68, 628, 915, 984
Bsp143I GATC 5 cut(s) 196, 294, 501, 837, 988
Bsp1720I GCTNAGC 1 cut(s) 617
BspACI CCGC 5 cut(s) 438, 754, 1011, 1083, 1091
BspANI GGCC 6 cut(s) 117, 210, 270, 449, 593, 692
BspCNI CTCAG 5 cut(s) 551, 573, 630, 984, 1086
BspFNI CGCG 1 cut(s) 729
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 5 cut(s) 641, 713, 942, 1031, 1088
BspPI GGATC 3 cut(s) 191, 845, 996
BsrFI RCCGGY 5 cut(s) 113, 206, 362, 589, 684
BsrI ACTGG 3 cut(s) 182, 271, 353
BssAI RCCGGY 5 cut(s) 113, 206, 362, 589, 684
BssECI CCNNGG 5 cut(s) 132, 150, 232, 889, 1045
BssMI GATC 5 cut(s) 196, 294, 501, 837, 988
BssSI CACGAG 1 cut(s) 527
BssT1I CCWWGG 1 cut(s) 132
Bst2BI CACGAG 1 cut(s) 527
Bst2UI CCWGG 4 cut(s) 151, 554, 698, 709
Bst4CI ACNGT 3 cut(s) 61, 323, 478
BstDEI CTNAG 5 cut(s) 538, 560, 617, 992, 1094
BstDSI CCRYGG 1 cut(s) 889
BstFNI CGCG 1 cut(s) 729
BstKTI GATC 5 cut(s) 199, 297, 504, 840, 991
BstMAI GTCTC 4 cut(s) 58, 329, 530, 746
BstMBI GATC 5 cut(s) 196, 294, 501, 837, 988
BstMCI CGRYCG 4 cut(s) 211, 322, 450, 594
BstNI CCWGG 4 cut(s) 151, 554, 698, 709
BstSCI CCNGG 9 cut(s) 149, 275, 552, 621, 650, 660, 696, 707, 949
BstSLI GKGCMC 1 cut(s) 984
BstUI CGCG 1 cut(s) 729
BstV1I GCAGC 4 cut(s) 104, 155, 179, 324
BstV2I GAAGAC 1 cut(s) 252
BstX2I RGATCY 3 cut(s) 501, 837, 988
BstYI RGATCY 3 cut(s) 501, 837, 988
BstZI CGGCCG 3 cut(s) 208, 447, 591
BsuRI GGCC 6 cut(s) 117, 210, 270, 449, 593, 692
BtgI CCRYGG 1 cut(s) 889
BtsIMutI CAGTG 1 cut(s) 66
CciI TCATGA 1 cut(s) 258
Cfr10I RCCGGY 5 cut(s) 113, 206, 362, 589, 684
Cfr13I GGNCC 4 cut(s) 478, 556, 700, 941
CseI GACGC 1 cut(s) 798
Csp6I GTAC 6 cut(s) 181, 634, 731, 901, 925, 1098
CspAI ACCGGT 1 cut(s) 684
CviAII CATG 2 cut(s) 259, 679
CviQI GTAC 6 cut(s) 181, 634, 731, 901, 925, 1098
DdeI CTNAG 5 cut(s) 538, 560, 617, 992, 1094
DpnI GATC 5 cut(s) 198, 296, 503, 839, 990
DpnII GATC 5 cut(s) 196, 294, 501, 837, 988
EaeI YGGCCR 4 cut(s) 208, 447, 591, 690
EagI CGGCCG 3 cut(s) 208, 447, 591
Ecl136II GAGCTC 1 cut(s) 66
EclXI CGGCCG 3 cut(s) 208, 447, 591
Eco130I CCWWGG 1 cut(s) 132
Eco24I GRGCYC 2 cut(s) 68, 628
Eco32I GATATC 1 cut(s) 454
Eco47I GGWCC 4 cut(s) 478, 556, 700, 941
Eco52I CGGCCG 3 cut(s) 208, 447, 591
Eco53kI GAGCTC 1 cut(s) 66
Eco88I CYCGRG 1 cut(s) 577
EcoICRI GAGCTC 1 cut(s) 66
EcoRII CCWGG 4 cut(s) 149, 552, 696, 707
EcoRV GATATC 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 132
EcoT38I GRGCYC 2 cut(s) 68, 628
ErhI CCWWGG 1 cut(s) 132
Esp3I CGTCTC 1 cut(s) 329
FaeI CATG 2 cut(s) 262, 682
FaiI YATR 7 cut(s) 260, 284, 680, 848, 875, 908, 975
FaqI GGGAC 1 cut(s) 796
FatI CATG 2 cut(s) 258, 678
FauI CCCGC 5 cut(s) 431, 747, 1004, 1076, 1098
FblI GTMKAC 1 cut(s) 219
Fnu4HI GCNGC 4 cut(s) 93, 169, 193, 313
FriOI GRGCYC 2 cut(s) 68, 628
Fsp4HI GCNGC 4 cut(s) 93, 169, 193, 313
FspBI CTAG 2 cut(s) 89, 341
GluI GCNGC 4 cut(s) 93, 169, 193, 313
HaeIII GGCC 6 cut(s) 117, 210, 270, 449, 593, 692
HgaI GACGC 1 cut(s) 798
Hin1II CATG 2 cut(s) 262, 682
HincII GTYRAC 1 cut(s) 220
HindII GTYRAC 1 cut(s) 220
HphI GGTGA 6 cut(s) 22, 112, 291, 319, 352, 640
Hpy166II GTNNAC 2 cut(s) 175, 220
Hpy188III TCNNGA 6 cut(s) 259, 466, 529, 610, 776, 841
Hpy8I GTNNAC 2 cut(s) 175, 220
Hpy99I CGWCG 4 cut(s) 221, 769, 967, 1060
HpyAV CCTTC 3 cut(s) 346, 860, 931
HpyCH4III ACNGT 3 cut(s) 61, 323, 478
HpyCH4IV ACGT 2 cut(s) 767, 882
HpyCH4V TGCA 2 cut(s) 312, 550
HpyF3I CTNAG 5 cut(s) 538, 560, 617, 992, 1094
HpySE526I ACGT 2 cut(s) 767, 882
Hsp92II CATG 2 cut(s) 262, 682
Kzo9I GATC 5 cut(s) 196, 294, 501, 837, 988
LmnI GCTCC 2 cut(s) 71, 1049
Lsp1109I GCAGC 4 cut(s) 104, 155, 179, 324
LweI GCATC 4 cut(s) 147, 459, 603, 1061
MaeI CTAG 2 cut(s) 89, 341
MaeII ACGT 2 cut(s) 767, 882
MaeIII GTNAC 2 cut(s) 358, 869
MalI GATC 5 cut(s) 198, 296, 503, 839, 990
MboI GATC 5 cut(s) 196, 294, 501, 837, 988
MboII GAAGA 3 cut(s) 16, 133, 257
MflI RGATCY 3 cut(s) 501, 837, 988
MhlI GDGCHC 4 cut(s) 68, 628, 915, 984
MluCI AATT 5 cut(s) 306, 716, 800, 895, 932
MlyI GAGTC 5 cut(s) 195, 222, 366, 532, 659
MmeI TCCRAC 2 cut(s) 136, 400
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 2 cut(s) 23, 225
MspA1I CMGCKG 2 cut(s) 192, 1093
MspR9I CCNGG 9 cut(s) 151, 277, 554, 623, 652, 662, 698, 709, 951
MvaI CCWGG 4 cut(s) 151, 554, 698, 709
MvnI CGCG 1 cut(s) 729
NciI CCSGG 5 cut(s) 277, 623, 652, 662, 951
NdeII GATC 5 cut(s) 196, 294, 501, 837, 988
NlaIII CATG 2 cut(s) 262, 682
NlaIV GGNNCC 5 cut(s) 641, 713, 942, 1031, 1088
NmeAIII GCCGAG 1 cut(s) 236
NmuCI GTSAC 1 cut(s) 358
PagI TCATGA 1 cut(s) 258
PcsI WCGNNNNNNNCGW 2 cut(s) 248, 342
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 9 cut(s) 125, 239, 370, 405, 442, 514, 772, 946, 1018
PfoI TCCNGGA 2 cut(s) 660, 707
PinAI ACCGGT 1 cut(s) 684
PkrI GCNGC 4 cut(s) 94, 170, 194, 314
PleI GAGTC 5 cut(s) 194, 221, 365, 531, 659
PpsI GAGTC 5 cut(s) 194, 221, 365, 531, 659
PshBI ATTAAT 1 cut(s) 225
Psp124BI GAGCTC 1 cut(s) 68
Psp6I CCWGG 4 cut(s) 149, 552, 696, 707
PspGI CCWGG 4 cut(s) 149, 552, 696, 707
PspN4I GGNNCC 5 cut(s) 641, 713, 942, 1031, 1088
PspPI GGNCC 4 cut(s) 478, 556, 700, 941
PsuI RGATCY 3 cut(s) 501, 837, 988
PvuII CAGCTG 1 cut(s) 192
RsaI GTAC 6 cut(s) 182, 635, 732, 902, 926, 1099
RsaNI GTAC 6 cut(s) 181, 634, 731, 901, 925, 1098
SacI GAGCTC 1 cut(s) 68
SalI GTCGAC 1 cut(s) 218
SaqAI TTAA 2 cut(s) 23, 225
SatI GCNGC 4 cut(s) 93, 169, 193, 313
Sau3AI GATC 5 cut(s) 196, 294, 501, 837, 988
Sau96I GGNCC 4 cut(s) 478, 556, 700, 941
SchI GAGTC 5 cut(s) 195, 222, 366, 532, 659
ScrFI CCNGG 9 cut(s) 151, 277, 554, 623, 652, 662, 698, 709, 951
SduI GDGCHC 4 cut(s) 68, 628, 915, 984
SfaNI GCATC 4 cut(s) 147, 459, 603, 1061
SinI GGWCC 4 cut(s) 478, 556, 700, 941
SmlI CTYRAG 2 cut(s) 464, 608
SmoI CTYRAG 2 cut(s) 464, 608
Sse9I AATT 5 cut(s) 306, 716, 800, 895, 932
SsiI CCGC 5 cut(s) 438, 754, 1011, 1083, 1091
SspMI CTAG 2 cut(s) 89, 341
SstI GAGCTC 1 cut(s) 68
StyD4I CCNGG 9 cut(s) 149, 275, 552, 621, 650, 660, 696, 707, 949
StyI CCWWGG 1 cut(s) 132
TaaI ACNGT 3 cut(s) 61, 323, 478
TaiI ACGT 2 cut(s) 770, 885
TaqI TCGA 7 cut(s) 219, 293, 318, 411, 764, 915, 1038
TasI AATT 5 cut(s) 306, 716, 800, 895, 932
TfiI GAWTC 9 cut(s) 125, 239, 370, 405, 442, 514, 772, 946, 1018
Tru1I TTAA 2 cut(s) 23, 225
Tru9I TTAA 2 cut(s) 23, 225
TscAI CASTG 1 cut(s) 66
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 4 cut(s) 92, 168, 192, 312
Tsp45I GTSAC 1 cut(s) 358
TspDTI ATGAA 3 cut(s) 17, 275, 695
TspGWI ACGGA 3 cut(s) 198, 795, 906
TspRI CASTG 1 cut(s) 66
VpaK11BI GGWCC 4 cut(s) 478, 556, 700, 941
VspI ATTAAT 1 cut(s) 225
XapI RAATTY 2 cut(s) 306, 895
XmiI GTMKAC 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 2 cut(s) 89, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.