pycom07g00540

DNA-damage-repair toleration protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0012848
Physical Location & Seq
Forward (+)
512305 .. 513610
1306 bp
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UTR
Exon/CDS
Intron
pycom07g00540.1

Sequence Viewer

Length: 1221 bp
ATGCACAGTGAACCTCTGCTGCCCTCACATCCTTGCTGCTCCTGCATATATATTATACATCTCACTCCATCAACTTCCAAACCCCCATCTCCCCTTCTCTCTCTCTCTCTCTCTCTCTCTCTCATGAAGATGATGACGTTGCTCTTAATCTCAGTGGCAACATTCTGTGCCATTGTCTCAACTGTGAGCTCTTCTTGTTCCCCATCAGACCTAGCAGCACTTCAATCCATCAGAACCGGACTCACCGATACCTCATTGGGCATCTTCAACTCTTGGGTCGGAACCGATTGCTGCGTAAACTGGTACGGAGTCAGCTGCGATCCCAAAACCGGCCGAGTCGTCGACATTAATCTCCGAGGCGAGTCGGAGGACCCCATACTCACGAAATCCGGTCAGTCCGGGTTCATGTCAGGATCGATCTCGCCCCAAATTTGCAGACTCGACCACCTCACCACCCTCGTTATCGCCGACTGGAAAGGAGTCACCGGCAAGATACCCCAATGCCTCACCACCCTCTCCAAACTCCGAATTCTCGACCTCGTCGGAAACAAGATTTCGGGAGAGATTCCGGCCGATATTGGCAACCTCAAGATGCTCACAGTCCTCAACCTCGCCGATAACCAGATCTCCGGTAAGATTCCGGCGAGTCTCGTTGGCCTCTCCGGGCTAATGCACATGGACCTCAGCAACAACCAAATCACGGGGGAGATACCGGCCGATTTCGGAAAACTAAAGATGCTGAGCCGGGCTCTGCTGAACCGAAACCAGCTCACCGGGTCGATCCCCGATTCCATTGGCAACATGAACCGGTTGGCCGACCTGGACCTGTCCAGGAACCAAATGTCGGGTTATGTACCGGACTGCCTTGGGAAAATGCAGGTTCTTTCGACGTTGAATTTGGACAGAAACTCGTTTTCGGGTCAATTGCCAGCGTCCCTTTTGAGCAATCGGGGTATGGGGATCTTAAATTTGAGCCGAAATGGGTTCGAAGGTAACATACCGGACGTTTTCCACGGAAATTCGTACTTTATGGCACTGGATTTATCGTACAACAATTTAAAGGGGCCGATACCCGGTTCATTGTCGGCGGCGAAATATATCGGGCACTTGGATCGATCAGCCACAACCACCTGTGCGGGGCGATTCCTGTGGGGAACCCGTTCGATCACCAGGAAGCGTCGTCGTTTACCAACAATGATTGCCTCTGCGGGAACCCGCTGA

Protein Analysis

407

Amino Acids

43.59

Weight (kDa)

9.0

Isoelectric Point (pI)

38.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 68 - 107 1.9e-09 Leucine rich repeat N-terminal domain
LRR_14 PF23598 142 - 247 2.9e-11 Leucine-rich repeat region
LRR_8 PF13855 199 - 257 2.9e-07 Leucine rich repeat
LRR_8 PF13855 252 - 304 1.6e-06 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012848)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G12610
fragaria_vesca FvH4_7g00720
malus_domestica MD02G1315200.v1.1 MD07G1006700.v1.1
prunus_persica Prupe.2G009800_v2.0.a1
pyrus_communis pycom02g26420 pycom07g00540
rosa_chinensis RchiOBHm_Chr1g0314931
rosa_laevigata RLG00000030754
rosa_multiflora Rmu_co8044478.1_g000001 Rmu_sc0005015.1_g000002 Rmu_sc0005168.1_g000009
rosa_roxburghii Rroxscaffold_4G00331670
rosa_rugosa Rorug01G0003100
rosa_samantha Rh1AG014000 Rh1BG005600 Rh1DG007600
rosa_wichuraiana Rw1G000660

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 868
AccI GTMKAC 1 cut(s) 342
AciI CCGC 4 cut(s) 1088, 1136, 1208, 1216
AclWI GGATC 5 cut(s) 314, 421, 775, 968, 1119
AcoI YGGCCR 4 cut(s) 331, 570, 714, 813
AcsI RAATTY 5 cut(s) 429, 528, 895, 967, 1018
AfaI GTAC 4 cut(s) 305, 855, 1025, 1049
AfiI CCNNNNNNNGG 5 cut(s) 329, 700, 844, 1073, 1137
AgeI ACCGGT 1 cut(s) 807
AgsI TTSAA 3 cut(s) 224, 268, 895
AjnI CCWGG 3 cut(s) 819, 830, 1169
AluBI AGCT 3 cut(s) 189, 315, 769
AluI AGCT 3 cut(s) 189, 315, 769
Alw21I GWGCWC 1 cut(s) 191
Alw26I GTCTC 2 cut(s) 181, 653
AlwI GGATC 5 cut(s) 314, 421, 775, 968, 1119
AoxI GGCC 6 cut(s) 331, 570, 655, 714, 813, 1064
ApeKI GCWGC 5 cut(s) 19, 36, 215, 291, 315
ApoI RAATTY 5 cut(s) 429, 528, 895, 967, 1018
ArsI GACNNNNNNTTYG 2 cut(s) 880, 912
AseI ATTAAT 1 cut(s) 348
AsiGI ACCGGT 1 cut(s) 807
Asp700I GAANNNNTTC 1 cut(s) 1159
AspS9I GGNCC 4 cut(s) 370, 679, 823, 1064
AsuC2I CCSGG 5 cut(s) 400, 664, 746, 775, 1074
AsuHPI GGTGA 6 cut(s) 235, 442, 475, 499, 763, 1159
AsuII TTCGAA 1 cut(s) 987
AvaII GGWCC 3 cut(s) 370, 679, 823
BaeGI GKGCMC 1 cut(s) 1107
BanII GRGCYC 2 cut(s) 191, 751
BarI GAAGNNNNNNTAC 2 cut(s) 981, 1013
Bbv12I GWGCWC 1 cut(s) 191
BbvCI CCTCAGC 1 cut(s) 683
BbvI GCAGC 5 cut(s) 6, 23, 227, 278, 302
BccI CCATC 4 cut(s) 76, 94, 211, 236
BcgI CGANNNNNNTGC 2 cut(s) 1094, 1128
BciT130I CCWGG 3 cut(s) 821, 832, 1171
BcnI CCSGG 5 cut(s) 400, 664, 746, 775, 1074
BcoDI GTCTC 2 cut(s) 181, 653
BfaI CTAG 1 cut(s) 212
BfuAI ACCTGC 1 cut(s) 868
BglII AGATCT 1 cut(s) 624
BisI GCNGC 6 cut(s) 20, 37, 216, 292, 316, 1089
BlpI GCTNAGC 1 cut(s) 740
BlsI GCNGC 6 cut(s) 21, 38, 217, 293, 317, 1090
Bme1390I CCNGG 8 cut(s) 400, 664, 746, 775, 821, 832, 1074, 1171
Bme18I GGWCC 3 cut(s) 370, 679, 823
BmgT120I GGNCC 4 cut(s) 370, 679, 823, 1064
BmiI GGNNCC 6 cut(s) 283, 372, 836, 1065, 1156, 1213
BmrFI CCNGG 8 cut(s) 400, 664, 746, 775, 821, 832, 1074, 1171
BmsI GCATC 3 cut(s) 270, 582, 726
BplI GAGNNNNNCTC 2 cut(s) 733, 765
Bpu10I CCTNAGC 1 cut(s) 683
Bpu1102I GCTNAGC 1 cut(s) 740
Bpu14I TTCGAA 1 cut(s) 987
BpuEI CTTGAG 1 cut(s) 572
BpuMI CCSGG 5 cut(s) 400, 664, 746, 775, 1074
Bsa29I ATCGAT 2 cut(s) 416, 1114
BsaJI CCNNGG 3 cut(s) 355, 865, 1012
BsaWI WCCGGW 6 cut(s) 236, 389, 629, 807, 856, 1000
BsaXI ACNNNNNCTCC 2 cut(s) 611, 641
Bsc4I CCNNNNNNNGG 5 cut(s) 329, 700, 844, 1073, 1137
Bse118I RCCGGY 4 cut(s) 329, 485, 712, 807
Bse1I ACTGG 3 cut(s) 305, 476, 1041
BseBI CCWGG 3 cut(s) 821, 832, 1171
BseCI ATCGAT 2 cut(s) 416, 1114
BseDI CCNNGG 3 cut(s) 355, 865, 1012
BseGI GGATG 1 cut(s) 28
BseLI CCNNNNNNNGG 5 cut(s) 329, 700, 844, 1073, 1137
BseMII CTCAG 3 cut(s) 165, 697, 731
BseNI ACTGG 3 cut(s) 305, 476, 1041
BseSI GKGCMC 1 cut(s) 1107
BseX3I CGGCCG 3 cut(s) 331, 570, 714
BseXI GCAGC 5 cut(s) 6, 23, 227, 278, 302
Bsh1285I CGRYCG 3 cut(s) 334, 573, 717
BshFI GGCC 6 cut(s) 333, 572, 657, 716, 815, 1066
BshTI ACCGGT 1 cut(s) 807
BshVI ATCGAT 2 cut(s) 416, 1114
BsiEI CGRYCG 3 cut(s) 334, 573, 717
BsiHKAI GWGCWC 1 cut(s) 191
BslFI GGGAC 1 cut(s) 919
BslI CCNNNNNNNGG 5 cut(s) 329, 700, 844, 1073, 1137
BsmAI GTCTC 2 cut(s) 181, 653
BsmFI GGGAC 1 cut(s) 919
BsnI GGCC 6 cut(s) 333, 572, 657, 716, 815, 1066
Bsp119I TTCGAA 1 cut(s) 987
Bsp1286I GDGCHC 3 cut(s) 191, 751, 1107
Bsp143I GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
Bsp1720I GCTNAGC 1 cut(s) 740
BspACI CCGC 4 cut(s) 1088, 1136, 1208, 1216
BspANI GGCC 6 cut(s) 333, 572, 657, 716, 815, 1066
BspCNI CTCAG 3 cut(s) 164, 696, 732
BspDI ATCGAT 2 cut(s) 416, 1114
BspHI TCATGA 1 cut(s) 123
BspLI GGNNCC 6 cut(s) 283, 372, 836, 1065, 1156, 1213
BspMI ACCTGC 1 cut(s) 868
BspPI GGATC 5 cut(s) 314, 421, 775, 968, 1119
BspQI GCTCTTC 1 cut(s) 196
BspT104I TTCGAA 1 cut(s) 987
BsrFI RCCGGY 4 cut(s) 329, 485, 712, 807
BsrI ACTGG 3 cut(s) 305, 476, 1041
BssAI RCCGGY 4 cut(s) 329, 485, 712, 807
BssECI CCNNGG 3 cut(s) 355, 865, 1012
BssMI GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
BssT1I CCWWGG 1 cut(s) 865
Bst2UI CCWGG 3 cut(s) 821, 832, 1171
Bst4CI ACNGT 3 cut(s) 8, 184, 601
Bst6I CTCTTC 1 cut(s) 196
BstBI TTCGAA 1 cut(s) 987
BstC8I GCNNGC 1 cut(s) 930
BstDEI CTNAG 3 cut(s) 151, 683, 740
BstDSI CCRYGG 1 cut(s) 1012
BstF5I GGATG 1 cut(s) 28
BstKTI GATC 9 cut(s) 322, 416, 420, 627, 783, 963, 1114, 1118, 1167
BstMAI GTCTC 2 cut(s) 181, 653
BstMBI GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
BstMCI CGRYCG 3 cut(s) 334, 573, 717
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstNI CCWGG 3 cut(s) 821, 832, 1171
BstSCI CCNGG 8 cut(s) 398, 662, 744, 773, 819, 830, 1072, 1169
BstSLI GKGCMC 1 cut(s) 1107
BstV1I GCAGC 5 cut(s) 6, 23, 227, 278, 302
BstX2I RGATCY 2 cut(s) 624, 960
BstYI RGATCY 2 cut(s) 624, 960
BstZI CGGCCG 3 cut(s) 331, 570, 714
Bsu15I ATCGAT 2 cut(s) 416, 1114
BsuRI GGCC 6 cut(s) 333, 572, 657, 716, 815, 1066
BsuTUI ATCGAT 2 cut(s) 416, 1114
BtgI CCRYGG 1 cut(s) 1012
BtsCI GGATG 1 cut(s) 28
BtsIMutI CAGTG 3 cut(s) 13, 159, 1034
BveI ACCTGC 1 cut(s) 868
Cac8I GCNNGC 1 cut(s) 930
CciI TCATGA 1 cut(s) 123
Cfr10I RCCGGY 4 cut(s) 329, 485, 712, 807
Cfr13I GGNCC 4 cut(s) 370, 679, 823, 1064
ClaI ATCGAT 2 cut(s) 416, 1114
CseI GACGC 2 cut(s) 921, 1166
Csp6I GTAC 4 cut(s) 304, 854, 1024, 1048
CspAI ACCGGT 1 cut(s) 807
CviAII CATG 4 cut(s) 124, 406, 676, 802
CviQI GTAC 4 cut(s) 304, 854, 1024, 1048
DdeI CTNAG 3 cut(s) 151, 683, 740
DpnI GATC 9 cut(s) 321, 415, 419, 626, 782, 962, 1113, 1117, 1166
DpnII GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
DraI TTTAAA 1 cut(s) 1059
EaeI YGGCCR 4 cut(s) 331, 570, 714, 813
EagI CGGCCG 3 cut(s) 331, 570, 714
Eam1104I CTCTTC 1 cut(s) 196
EarI CTCTTC 1 cut(s) 196
Ecl136II GAGCTC 1 cut(s) 189
EclXI CGGCCG 3 cut(s) 331, 570, 714
Eco130I CCWWGG 1 cut(s) 865
Eco24I GRGCYC 2 cut(s) 191, 751
Eco47I GGWCC 3 cut(s) 370, 679, 823
Eco52I CGGCCG 3 cut(s) 331, 570, 714
Eco53kI GAGCTC 1 cut(s) 189
EcoICRI GAGCTC 1 cut(s) 189
EcoO109I RGGNCCY 1 cut(s) 370
EcoRI GAATTC 1 cut(s) 528
EcoRII CCWGG 3 cut(s) 819, 830, 1169
EcoT14I CCWWGG 1 cut(s) 865
EcoT38I GRGCYC 2 cut(s) 191, 751
ErhI CCWWGG 1 cut(s) 865
FaeI CATG 4 cut(s) 127, 409, 679, 805
FaqI GGGAC 1 cut(s) 919
FatI CATG 4 cut(s) 123, 405, 675, 801
FauI CCCGC 2 cut(s) 1129, 1201
FblI GTMKAC 1 cut(s) 342
Fnu4HI GCNGC 6 cut(s) 20, 37, 216, 292, 316, 1089
FokI GGATG 1 cut(s) 15
FriOI GRGCYC 2 cut(s) 191, 751
Fsp4HI GCNGC 6 cut(s) 20, 37, 216, 292, 316, 1089
FspBI CTAG 1 cut(s) 212
GluI GCNGC 6 cut(s) 20, 37, 216, 292, 316, 1089
HaeIII GGCC 6 cut(s) 333, 572, 657, 716, 815, 1066
HgaI GACGC 2 cut(s) 921, 1166
Hin1II CATG 4 cut(s) 127, 409, 679, 805
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HphI GGTGA 6 cut(s) 235, 442, 475, 499, 763, 1159
Hpy166II GTNNAC 4 cut(s) 11, 298, 343, 1187
Hpy188I TCNGA 8 cut(s) 208, 233, 281, 356, 367, 527, 545, 725
Hpy188III TCNNGA 6 cut(s) 124, 382, 411, 533, 558, 589
Hpy8I GTNNAC 4 cut(s) 11, 298, 343, 1187
Hpy99I CGWCG 5 cut(s) 344, 545, 892, 1182, 1185
HpyAV CCTTC 2 cut(s) 104, 983
HpyCH4III ACNGT 3 cut(s) 8, 184, 601
HpyCH4IV ACGT 3 cut(s) 137, 890, 1005
HpyCH4V TGCA 5 cut(s) 4, 45, 435, 673, 877
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 3 cut(s) 151, 683, 740
HpySE526I ACGT 3 cut(s) 137, 890, 1005
Hsp92II CATG 4 cut(s) 127, 409, 679, 805
Kzo9I GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
LguI GCTCTTC 1 cut(s) 196
LmnI GCTCC 1 cut(s) 44
Lsp1109I GCAGC 5 cut(s) 6, 23, 227, 278, 302
LweI GCATC 3 cut(s) 270, 582, 726
MaeI CTAG 1 cut(s) 212
MaeII ACGT 3 cut(s) 137, 890, 1005
MaeIII GTNAC 2 cut(s) 481, 992
MalI GATC 9 cut(s) 321, 415, 419, 626, 782, 962, 1113, 1117, 1166
MboI GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
MboII GAAGA 3 cut(s) 139, 183, 256
MfeI CAATTG 1 cut(s) 923
MflI RGATCY 2 cut(s) 624, 960
MhlI GDGCHC 3 cut(s) 191, 751, 1107
MluCI AATT 7 cut(s) 429, 528, 895, 923, 967, 1018, 1054
MlyI GAGTC 7 cut(s) 234, 318, 345, 371, 432, 489, 655
MmeI TCCRAC 3 cut(s) 259, 345, 523
MroXI GAANNNNTTC 1 cut(s) 1159
MseI TTAA 4 cut(s) 146, 348, 965, 1058
MslI CAYNNNNRTG 1 cut(s) 128
MspA1I CMGCKG 2 cut(s) 315, 1218
MspR9I CCNGG 8 cut(s) 400, 664, 746, 775, 821, 832, 1074, 1171
MunI CAATTG 1 cut(s) 923
MvaI CCWGG 3 cut(s) 821, 832, 1171
MwoI GCNNNNNNNGC 1 cut(s) 42
NciI CCSGG 5 cut(s) 400, 664, 746, 775, 1074
NdeII GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
NlaIII CATG 4 cut(s) 127, 409, 679, 805
NlaIV GGNNCC 6 cut(s) 283, 372, 836, 1065, 1156, 1213
NmeAIII GCCGAG 1 cut(s) 359
NmuCI GTSAC 1 cut(s) 481
NspV TTCGAA 1 cut(s) 987
PagI TCATGA 1 cut(s) 123
PciSI GCTCTTC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 1 cut(s) 465
PdmI GAANNNNTTC 1 cut(s) 1159
PfeI GAWTC 4 cut(s) 565, 637, 788, 1143
PflFI GACNNNGTC 1 cut(s) 539
PfoI TCCNGGA 1 cut(s) 830
PinAI ACCGGT 1 cut(s) 807
PkrI GCNGC 6 cut(s) 21, 38, 217, 293, 317, 1090
PleI GAGTC 7 cut(s) 234, 317, 344, 370, 432, 488, 654
PpsI GAGTC 7 cut(s) 234, 317, 344, 370, 432, 488, 654
PpuMI RGGWCCY 1 cut(s) 370
PshBI ATTAAT 1 cut(s) 348
Psp124BI GAGCTC 1 cut(s) 191
Psp5II RGGWCCY 1 cut(s) 370
Psp6I CCWGG 3 cut(s) 819, 830, 1169
PspGI CCWGG 3 cut(s) 819, 830, 1169
PspN4I GGNNCC 6 cut(s) 283, 372, 836, 1065, 1156, 1213
PspPI GGNCC 4 cut(s) 370, 679, 823, 1064
PspPPI RGGWCCY 1 cut(s) 370
PsuI RGATCY 2 cut(s) 624, 960
PsyI GACNNNGTC 1 cut(s) 539
PvuII CAGCTG 1 cut(s) 315
RsaI GTAC 4 cut(s) 305, 855, 1025, 1049
RsaNI GTAC 4 cut(s) 304, 854, 1024, 1048
RseI CAYNNNNRTG 1 cut(s) 128
SacI GAGCTC 1 cut(s) 191
SalI GTCGAC 1 cut(s) 341
SapI GCTCTTC 1 cut(s) 196
SaqAI TTAA 4 cut(s) 146, 348, 965, 1058
SatI GCNGC 6 cut(s) 20, 37, 216, 292, 316, 1089
Sau3AI GATC 9 cut(s) 319, 413, 417, 624, 780, 960, 1111, 1115, 1164
Sau96I GGNCC 4 cut(s) 370, 679, 823, 1064
SchI GAGTC 7 cut(s) 234, 318, 345, 371, 432, 489, 655
ScrFI CCNGG 8 cut(s) 400, 664, 746, 775, 821, 832, 1074, 1171
SduI GDGCHC 3 cut(s) 191, 751, 1107
SfaNI GCATC 3 cut(s) 270, 582, 726
SfuI TTCGAA 1 cut(s) 987
SinI GGWCC 3 cut(s) 370, 679, 823
SmiMI CAYNNNNRTG 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 587
SmoI CTYRAG 1 cut(s) 587
Sse9I AATT 7 cut(s) 429, 528, 895, 923, 967, 1018, 1054
SsiI CCGC 4 cut(s) 1088, 1136, 1208, 1216
SspMI CTAG 1 cut(s) 212
SstI GAGCTC 1 cut(s) 191
StyD4I CCNGG 8 cut(s) 398, 662, 744, 773, 819, 830, 1072, 1169
StyI CCWWGG 1 cut(s) 865
TaaI ACNGT 3 cut(s) 8, 184, 601
TaiI ACGT 3 cut(s) 140, 893, 1008
TaqI TCGA 9 cut(s) 342, 416, 441, 534, 779, 887, 987, 1114, 1163
TasI AATT 7 cut(s) 429, 528, 895, 923, 967, 1018, 1054
TauI GCSGC 1 cut(s) 1091
TfiI GAWTC 4 cut(s) 565, 637, 788, 1143
Tru1I TTAA 4 cut(s) 146, 348, 965, 1058
Tru9I TTAA 4 cut(s) 146, 348, 965, 1058
TscAI CASTG 3 cut(s) 13, 159, 1041
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 5 cut(s) 19, 36, 215, 291, 315
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 4 cut(s) 140, 394, 818, 1068
TspGWI ACGGA 2 cut(s) 321, 1029
TspRI CASTG 3 cut(s) 13, 159, 1041
Tth111I GACNNNGTC 1 cut(s) 539
VpaK11BI GGWCC 3 cut(s) 370, 679, 823
VspI ATTAAT 1 cut(s) 348
XapI RAATTY 5 cut(s) 429, 528, 895, 967, 1018
XmiI GTMKAC 1 cut(s) 342
XmnI GAANNNNTTC 1 cut(s) 1159
XspI CTAG 1 cut(s) 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.