MD07G1061500.v1.1
NAC Family

inactive poly ADP-ribose polymerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0002330
Physical Location & Seq
Reverse (-)
5745854 .. 5753695
7842 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1061500.v1.1.491

Sequence Viewer

Length: 1803 bp
ATGGAAGCAAAGGTCGCAAAGGTATTGGATAGTGGTCGAAGAGTTATGCTTGACTTAAAGAGAAAGCGGGCAACCCGGTATGCAACATATTTTCCTGGAGCTACACACAGAGTGTCACAGAGGCCGAGCTTGAATAAGCTTGCCAAGCGAAGGAAATTATATGGATGCAAAAGTAAGCTTATGAGCCGTGGATCTCAACGATCCTTACTTGTATCTTATTCAAATTTTTCAAGGAGTGGTGTGCCACAGCGTCTAATGTTTTATCAAAGTGGTGAATGGACTGATTTTCCTCAGGATCTTGTTGACTTGGTTAGGAAAGACCTGCAGGTCAAGAAGGAAACTGTGAACATCAAATCACATGGTCAACATTATGTGCTAGATTTTTTGCATATGTTCCGATTGGACTTGAAAACTGGTCTACAGCAACCTATTGCTTGGATTGATGAAGCTGGTAGTTGTTTCTTTCCAGAAATTTATGCTGAGGACGATGATGAACCAAACTGGTACTGCCCACCTGAAGGTGGCAAAGGTTTGGAACCCTGGGCTGAAGATCAGTGTGAATCACATGAGATAAGACTGCACCTGGAAATTGAAATAAATGGTATGGGTCAATCTGGGTTGAATGAATGTAGTGGGGAGTCGAATGGCTTTGTAAAGAAAATTCAGGTTGATCAAATACCTGCCAGCAATCGTGATGGTATAGAAGTTGAGAATAGTTGCAACAGGAAACCTGGTCTAAACGATGATGCATATTCTGATGACAATGAAGCAATGACAAAGAATTTAGCTAGAGCAACTGAGATTGAAAAGGAAAAGTTGGATTGTGATACTGTGCGTAAGATATTCCTTAAAGGAATGAGCAACTTTGATAGTGTAGATGTACTTGATATATACAAATGCTCAAGTACTCTGATGCAAGCTCGATTGGAACTTTTTCAGAAGCAGGTAGAAATTACGAAAACGTTTCGTGGTGATGCAAATATACGATACGCTTGGCTTGCTTCTTCTAAAGGAGAGCTGGCAACTATAATGATGTATGGGCTTGGTCATTCTGGGCTAGCCACGAACAAGTCCATATATGGCACTGGTGTTCATCTCACAGAAGCTAGCTTCTCTAACATCTGTGCAAGTTCTTGTGATGTTGACGAAAATGGGGCACGACACATGATATTATGTCGTGTCATAATGGGAAATATGGAGTTACTTCACCCTGGAAGTAAGCAATTCCATCCCAGTAGCAAAGACTTTGATAGTGGGGTGGATGGTCTTCAGGATCCAAAACATTATATTGTCTGGAATATGAACATGAATACACACATATATCCAGAATTTGTTGTTAGTTTCAAGATCACATCCAATACTGAAGGACATCTAATTGGAACTGAGAATAAACTTGGTGCTTCCGGGGTTGGGACATCCTGCCAAGGACCACAGGGCTCGTCTGCAGTTGATACAGGGAGCGAGACTCAGCCACTTTCTGATTCTGGGAAATCTCATGGAAACAATAGTCAAATGATTTCAAAGCCTGGAAGATTTCAGGGGGAAACCACTAATACTGGTTCTGCTCCTCAAAGAACCCCTAAATCACCCTGGATGCCTTTCCCCATGTTGTTTTCTGCTATTGAAAATAAGGTTCCTCCTGAAGATATGAAACAAGTTAATGTTCATTATGACTTGTTCAGGGAAAGAAAGATAACTCGTGATGAATTTGTGAAAAAGTTGAGGTTGGTTGTTGGAGATACTTTGTTGAGGTCTACGATTACAGAACTTCAGTGTAAGATACCTCATAAAAGCAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000160 GO:0000302 GO:0000303 GO:0000305 GO:0001101 GO:0001505 GO:0002376 GO:0003006 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006807 GO:0006809 GO:0006950 GO:0006952 GO:0006955 GO:0006970 GO:0006979 GO:0007154 GO:0007165 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0009058 GO:0009314 GO:0009414 GO:0009415 GO:0009416 GO:0009605 GO:0009607 GO:0009617 GO:0009628 GO:0009648 GO:0009651 GO:0009653 GO:0009719 GO:0009723 GO:0009725 GO:0009753 GO:0009755 GO:0009790 GO:0009791 GO:0009793 GO:0009814 GO:0009816 GO:0009867 GO:0009873 GO:0009987 GO:0010015 GO:0010033 GO:0010035 GO:0010101 GO:0010102 GO:0010154 GO:0010193 GO:0010228 GO:0012501 GO:0017144 GO:0019222 GO:0022414 GO:0022622 GO:0023052 GO:0031323 GO:0032501 GO:0032502 GO:0032870 GO:0034641 GO:0035556 GO:0042133 GO:0042136 GO:0042221 GO:0042493 GO:0042742 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044249 GO:0044271 GO:0044424 GO:0044464 GO:0045087 GO:0046209 GO:0046677 GO:0048316 GO:0048364 GO:0048527 GO:0048528 GO:0048573 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0065007 GO:0065008 GO:0070887 GO:0071229 GO:0071310 GO:0071369 GO:0071395 GO:0071495 GO:0072593 GO:0090696 GO:0090697 GO:0090698 GO:0098542 GO:0099402 GO:1901700 GO:1901701 GO:1903409 GO:1905392 GO:2000377 GO:2001057
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PARP RST

Protein Analysis

601

Amino Acids

67.34

Weight (kDa)

6.99

Isoelectric Point (pI)

46.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WWE_5 PF23467 76 - 155 2.3e-36 WWE domain
RST PF12174 527 - 591 3.2e-29 RCD1-SRO-TAF4 (RST) plant domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 326
Acc36I ACCTGC 4 cut(s) 316, 330, 688, 934
AccI GTMKAC 2 cut(s) 418, 1754
AciI CCGC 1 cut(s) 67
AclI AACGTT 1 cut(s) 962
AclWI GGATC 5 cut(s) 195, 199, 303, 1268, 1281
AcsI RAATTY 6 cut(s) 223, 471, 660, 781, 1328, 1706
AcuI CTGAAG 6 cut(s) 537, 567, 1253, 1383, 1662, 1754
AdeI CACNNNGTG 1 cut(s) 112
AfaI GTAC 3 cut(s) 506, 882, 907
AfiI CCNNNNNNNGG 4 cut(s) 150, 518, 521, 1410
AjnI CCWGG 7 cut(s) 94, 539, 582, 730, 1210, 1525, 1589
Alw26I GTCTC 1 cut(s) 1457
AlwI GGATC 5 cut(s) 195, 199, 303, 1268, 1281
AoxI GGCC 1 cut(s) 122
ApoI RAATTY 6 cut(s) 223, 471, 660, 781, 1328, 1706
Asp700I GAANNNNTTC 1 cut(s) 933
AspS9I GGNCC 1 cut(s) 1427
AsuC2I CCSGG 2 cut(s) 76, 1405
AsuHPI GGTGA 4 cut(s) 284, 983, 1199, 1578
AsuNHI GCTAGC 2 cut(s) 1057, 1106
AvaII GGWCC 1 cut(s) 1427
AxyI CCTNAGG 1 cut(s) 291
BaeGI GKGCMC 1 cut(s) 1159
BamHI GGATCC 1 cut(s) 1273
BanII GRGCYC 1 cut(s) 1439
BauI CACGAG 1 cut(s) 1698
BbsI GAAGAC 1 cut(s) 1259
BbvCI CCTCAGC 1 cut(s) 480
BccI CCATC 3 cut(s) 689, 1236, 1256
BceAI ACGGC 1 cut(s) 171
BciT130I CCWGG 7 cut(s) 96, 541, 584, 732, 1212, 1527, 1591
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 2 cut(s) 76, 1405
BcoDI GTCTC 1 cut(s) 1457
BfaI CTAG 4 cut(s) 377, 789, 1058, 1107
BfmI CTRYAG 3 cut(s) 323, 419, 1443
BfuAI ACCTGC 4 cut(s) 316, 330, 688, 934
BmcAI AGTACT 1 cut(s) 907
Bme1390I CCNGG 9 cut(s) 76, 96, 541, 584, 732, 1212, 1405, 1527, 1591
Bme18I GGWCC 1 cut(s) 1427
BmgT120I GGNCC 1 cut(s) 1427
BmiI GGNNCC 3 cut(s) 537, 1275, 1635
BmrFI CCNGG 9 cut(s) 76, 96, 541, 584, 732, 1212, 1405, 1527, 1591
BmrI ACTGGG 1 cut(s) 1227
BmsI GCATC 5 cut(s) 155, 736, 903, 964, 1584
BmtI GCTAGC 2 cut(s) 1061, 1110
BmuI ACTGGG 1 cut(s) 1227
BpiI GAAGAC 1 cut(s) 1259
BplI GAGNNNNNCTC 2 cut(s) 1450, 1482
BpmI CTGGAG 1 cut(s) 117
Bpu10I CCTNAGC 1 cut(s) 480
BpuEI CTTGAG 1 cut(s) 886
BpuMI CCSGG 2 cut(s) 76, 1405
BsaJI CCNNGG 7 cut(s) 187, 539, 540, 1210, 1404, 1423, 1589
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 518, 521, 1410
Bse1I ACTGG 5 cut(s) 418, 506, 1090, 1233, 1561
Bse21I CCTNAGG 1 cut(s) 291
Bse3DI GCAATG 2 cut(s) 777, 1801
BseBI CCWGG 7 cut(s) 96, 541, 584, 732, 1212, 1527, 1591
BseDI CCNNGG 7 cut(s) 187, 539, 540, 1210, 1404, 1423, 1589
BseGI GGATG 6 cut(s) 170, 1228, 1267, 1352, 1415, 1599
BseLI CCNNNNNNNGG 4 cut(s) 150, 518, 521, 1410
BseMI GCAATG 2 cut(s) 777, 1801
BseMII CTCAG 5 cut(s) 305, 471, 789, 1374, 1481
BseNI ACTGG 5 cut(s) 418, 506, 1090, 1233, 1561
BseRI GAGGAG 1 cut(s) 1557
BseSI GKGCMC 1 cut(s) 1159
BsgI GTGCAG 1 cut(s) 563
BshFI GGCC 1 cut(s) 124
BsiSI CCGG 2 cut(s) 76, 1404
BslFI GGGAC 1 cut(s) 1426
BslI CCNNNNNNNGG 4 cut(s) 150, 518, 521, 1410
BsmAI GTCTC 1 cut(s) 1457
BsmFI GGGAC 1 cut(s) 1426
BsnI GGCC 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 1159, 1439
Bsp143I GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
BspACI CCGC 1 cut(s) 67
BspANI GGCC 1 cut(s) 124
BspCNI CTCAG 5 cut(s) 304, 472, 790, 1375, 1480
BspLI GGNNCC 3 cut(s) 537, 1275, 1635
BspMAI CTGCAG 2 cut(s) 327, 1447
BspMI ACCTGC 4 cut(s) 316, 330, 688, 934
BspOI GCTAGC 2 cut(s) 1061, 1110
BspPI GGATC 5 cut(s) 195, 199, 303, 1268, 1281
BsrDI GCAATG 2 cut(s) 777, 1801
BsrI ACTGG 5 cut(s) 418, 506, 1090, 1233, 1561
BssECI CCNNGG 7 cut(s) 187, 539, 540, 1210, 1404, 1423, 1589
BssMI GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
BssSI CACGAG 1 cut(s) 1698
BssT1I CCWWGG 1 cut(s) 1423
Bst2BI CACGAG 1 cut(s) 1698
Bst2UI CCWGG 7 cut(s) 96, 541, 584, 732, 1212, 1527, 1591
Bst4CI ACNGT 2 cut(s) 343, 832
Bst6I CTCTTC 1 cut(s) 34
BstC8I GCNNGC 8 cut(s) 69, 141, 685, 918, 999, 1020, 1059, 1108
BstDEI CTNAG 5 cut(s) 291, 480, 798, 1383, 1467
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 6 cut(s) 170, 1228, 1267, 1352, 1415, 1599
BstKTI GATC 7 cut(s) 194, 203, 298, 553, 673, 1276, 1350
BstMAI GTCTC 1 cut(s) 1457
BstMBI GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
BstMWI GCNNNNNNNGC 3 cut(s) 14, 145, 998
BstNI CCWGG 7 cut(s) 96, 541, 584, 732, 1212, 1527, 1591
BstSCI CCNGG 9 cut(s) 74, 94, 539, 582, 730, 1210, 1403, 1525, 1589
BstSFI CTRYAG 3 cut(s) 323, 419, 1443
BstSLI GKGCMC 1 cut(s) 1159
BstV2I GAAGAC 1 cut(s) 1259
BstX2I RGATCY 3 cut(s) 191, 295, 1273
BstYI RGATCY 3 cut(s) 191, 295, 1273
Bsu36I CCTNAGG 1 cut(s) 291
BsuRI GGCC 1 cut(s) 124
BtgI CCRYGG 1 cut(s) 187
BtsCI GGATG 6 cut(s) 170, 1228, 1267, 1352, 1415, 1599
BtsIMutI CAGTG 3 cut(s) 560, 1083, 1778
BveI ACCTGC 4 cut(s) 316, 330, 688, 934
Cac8I GCNNGC 8 cut(s) 69, 141, 685, 918, 999, 1020, 1059, 1108
Cfr13I GGNCC 1 cut(s) 1427
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 730
Csp6I GTAC 3 cut(s) 505, 881, 906
CviAII CATG 6 cut(s) 359, 566, 1165, 1306, 1496, 1606
CviQI GTAC 3 cut(s) 505, 881, 906
DdeI CTNAG 5 cut(s) 291, 480, 798, 1383, 1467
DpnI GATC 7 cut(s) 193, 202, 297, 552, 672, 1275, 1349
DpnII GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
DraIII CACNNNGTG 1 cut(s) 112
DrdI GACNNNNNNGTC 1 cut(s) 326
DseDI GACNNNNNNGTC 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 1 cut(s) 1423
Eco24I GRGCYC 1 cut(s) 1439
Eco47I GGWCC 1 cut(s) 1427
Eco57I CTGAAG 6 cut(s) 537, 567, 1253, 1383, 1662, 1754
Eco81I CCTNAGG 1 cut(s) 291
EcoRII CCWGG 7 cut(s) 94, 539, 582, 730, 1210, 1525, 1589
EcoT14I CCWWGG 1 cut(s) 1423
EcoT22I ATGCAT 1 cut(s) 751
EcoT38I GRGCYC 1 cut(s) 1439
ErhI CCWWGG 1 cut(s) 1423
FaeI CATG 6 cut(s) 362, 569, 1168, 1309, 1499, 1609
FaqI GGGAC 1 cut(s) 1426
FatI CATG 6 cut(s) 358, 565, 1164, 1305, 1495, 1605
FauI CCCGC 1 cut(s) 60
FauNDI CATATG 1 cut(s) 390
FbaI TGATCA 1 cut(s) 670
FblI GTMKAC 2 cut(s) 418, 1754
FokI GGATG 6 cut(s) 177, 1215, 1274, 1339, 1402, 1606
FriOI GRGCYC 1 cut(s) 1439
FspBI CTAG 4 cut(s) 377, 789, 1058, 1107
GsuI CTGGAG 1 cut(s) 117
HaeIII GGCC 1 cut(s) 124
HapII CCGG 2 cut(s) 76, 1404
HgaI GACGC 1 cut(s) 239
Hin1II CATG 6 cut(s) 362, 569, 1168, 1309, 1499, 1609
HincII GTYRAC 3 cut(s) 304, 365, 1144
HindII GTYRAC 3 cut(s) 304, 365, 1144
HindIII AAGCTT 2 cut(s) 137, 176
HinfI GANTC 4 cut(s) 560, 638, 1465, 1481
HpaII CCGG 2 cut(s) 76, 1404
HphI GGTGA 4 cut(s) 284, 983, 1199, 1578
Hpy166II GTNNAC 6 cut(s) 304, 346, 365, 419, 1144, 1755
Hpy188I TCNGA 5 cut(s) 398, 757, 912, 939, 1480
Hpy8I GTNNAC 6 cut(s) 304, 346, 365, 419, 1144, 1755
HpyAV CCTTC 4 cut(s) 144, 328, 512, 1358
HpyCH4III ACNGT 2 cut(s) 343, 832
HpyCH4IV ACGT 1 cut(s) 962
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 145, 998
HpyF3I CTNAG 5 cut(s) 291, 480, 798, 1383, 1467
HpySE526I ACGT 1 cut(s) 962
Hsp92II CATG 6 cut(s) 362, 569, 1168, 1309, 1499, 1609
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
LmnI GCTCC 3 cut(s) 98, 1458, 1570
LweI GCATC 5 cut(s) 155, 736, 903, 964, 1584
MabI ACCWGGT 1 cut(s) 730
MaeI CTAG 4 cut(s) 377, 789, 1058, 1107
MaeII ACGT 1 cut(s) 962
MaeIII GTNAC 2 cut(s) 114, 1200
MalI GATC 7 cut(s) 193, 202, 297, 552, 672, 1275, 1349
MboI GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
MboII GAAGA 6 cut(s) 51, 560, 996, 1259, 1542, 1655
MflI RGATCY 3 cut(s) 191, 295, 1273
MhlI GDGCHC 2 cut(s) 1159, 1439
MlyI GAGTC 2 cut(s) 647, 1459
MmeI TCCRAC 2 cut(s) 798, 1714
MnlI CCTC 8 cut(s) 114, 300, 475, 1578, 1647, 1716, 1743, 1794
Mph1103I ATGCAT 1 cut(s) 751
MroXI GAANNNNTTC 1 cut(s) 933
MseI TTAA 3 cut(s) 56, 849, 1659
MspI CCGG 2 cut(s) 76, 1404
MspR9I CCNGG 9 cut(s) 76, 96, 541, 584, 732, 1212, 1405, 1527, 1591
MvaI CCWGG 7 cut(s) 96, 541, 584, 732, 1212, 1527, 1591
MwoI GCNNNNNNNGC 3 cut(s) 14, 145, 998
NciI CCSGG 2 cut(s) 76, 1405
NdeI CATATG 1 cut(s) 390
NdeII GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
NheI GCTAGC 2 cut(s) 1057, 1106
NlaIII CATG 6 cut(s) 362, 569, 1168, 1309, 1499, 1609
NlaIV GGNNCC 3 cut(s) 537, 1275, 1635
NmeAIII GCCGAG 1 cut(s) 150
NmuCI GTSAC 1 cut(s) 114
NsiI ATGCAT 1 cut(s) 751
PasI CCCWGGG 1 cut(s) 540
PdmI GAANNNNTTC 1 cut(s) 933
PfeI GAWTC 2 cut(s) 560, 1481
PfoI TCCNGGA 1 cut(s) 94
PleI GAGTC 2 cut(s) 646, 1459
PpsI GAGTC 2 cut(s) 646, 1459
Psp1406I AACGTT 1 cut(s) 962
Psp6I CCWGG 7 cut(s) 94, 539, 582, 730, 1210, 1525, 1589
PspGI CCWGG 7 cut(s) 94, 539, 582, 730, 1210, 1525, 1589
PspN4I GGNNCC 3 cut(s) 537, 1275, 1635
PspPI GGNCC 1 cut(s) 1427
PstI CTGCAG 2 cut(s) 327, 1447
PsuI RGATCY 3 cut(s) 191, 295, 1273
RsaI GTAC 3 cut(s) 506, 882, 907
RsaNI GTAC 3 cut(s) 505, 881, 906
SaqAI TTAA 3 cut(s) 56, 849, 1659
Sau3AI GATC 7 cut(s) 191, 200, 295, 550, 670, 1273, 1347
Sau96I GGNCC 1 cut(s) 1427
SbfI CCTGCAGG 1 cut(s) 327
ScaI AGTACT 1 cut(s) 907
SchI GAGTC 2 cut(s) 647, 1459
ScrFI CCNGG 9 cut(s) 76, 96, 541, 584, 732, 1212, 1405, 1527, 1591
SdaI CCTGCAGG 1 cut(s) 327
SduI GDGCHC 2 cut(s) 1159, 1439
SexAI ACCWGGT 1 cut(s) 730
SfaNI GCATC 5 cut(s) 155, 736, 903, 964, 1584
SfcI CTRYAG 3 cut(s) 323, 419, 1443
SinI GGWCC 1 cut(s) 1427
SmlI CTYRAG 1 cut(s) 901
SmoI CTYRAG 1 cut(s) 901
Sse8387I CCTGCAGG 1 cut(s) 327
SsiI CCGC 1 cut(s) 67
SspMI CTAG 4 cut(s) 377, 789, 1058, 1107
StyD4I CCNGG 9 cut(s) 74, 94, 539, 582, 730, 1210, 1403, 1525, 1589
StyI CCWWGG 1 cut(s) 1423
TaaI ACNGT 2 cut(s) 343, 832
TaiI ACGT 1 cut(s) 965
TaqI TCGA 3 cut(s) 37, 641, 922
TatI WGTACW 2 cut(s) 880, 905
TfiI GAWTC 2 cut(s) 560, 1481
Tru1I TTAA 3 cut(s) 56, 849, 1659
Tru9I TTAA 3 cut(s) 56, 849, 1659
TscAI CASTG 3 cut(s) 560, 1090, 1778
TseFI GTSAC 1 cut(s) 114
Tsp45I GTSAC 1 cut(s) 114
TspRI CASTG 3 cut(s) 560, 1090, 1778
VpaK11BI GGWCC 1 cut(s) 1427
XapI RAATTY 6 cut(s) 223, 471, 660, 781, 1328, 1706
XmiI GTMKAC 2 cut(s) 418, 1754
XmnI GAANNNNTTC 1 cut(s) 933
XspI CTAG 4 cut(s) 377, 789, 1058, 1107
ZrmI AGTACT 1 cut(s) 907
Zsp2I ATGCAT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.