MD07G1063700.v1.1

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014353
Physical Location & Seq
Forward (+)
5984642 .. 5990557
5916 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1063700.v1.1.491

Sequence Viewer

Length: 876 bp
ATGGCGCTCTTTGCTTCTTCCATCAATCTCCATCTATCTAAAATCTCTCATTCCGAATTTGCCCCTCGGCCCCGCCCCTCCCCGTACGACCCCCCCAAACTCCTCCCTTTTCCTGCCGTATGCTCCTCCCAACGTGCTGCCGCCAACGCTTTCTCCAGAGAAAGGAACATTATGCGTGCGTCGATGAATGGTCTGAATGCAGTTGGATCAAGCTCGTTGAAGTCTGATCAAGGTGCTGATTATCTTCCAATGCCAGTTGTTATGATCGATCAAGATTCAGATTCTGATGCAACTATTGTGCAACTCAGCTTCGGCGATCGCCTTGGAGCACTCATTGATACGATGAATGCATTGAAAGATTTGGGATTGGATGTGGCTAAGGGAACTGTCACCACTGAGGGACCAGTCAAACAAACGAAGTTTTATATAACACGATTAGACACTGGGCGCAAAGTTGAAGATCCCGATATGTTAGAGAGAATTCGACTTACCATCATCAACAATCTTTTGAAGTATCATCCAGAATCTAGTCAGCATCTAGCAATGGGTGAAGCATTTGGAATAAAAGCTCCAGAGAAGAAGCTTGATGTGGATATTGCAACTCATATACATGTGAAGGAAGATGGACCTAAGAAGAGCTTGCTTTACATAGAGACAGCAGATAGACCTGGGTTGCTAGTAGAAATCATAAAAATCATTGCTGACGTTAATATCGATGTGGAATCAGCTGAGATTGATACAGAAGGGTTGGTAGCCAAAGATAAATTTCATGTTAGCTACAGAGGGGCAGCATTAAACAGTTCCTTGTCTCAGGTGTTGGTGAACTGTTTGCGTTACTACATCCGGAGGCCAGAAACAGATATAGATAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

292

Amino Acids

32.07

Weight (kDa)

5.83

Isoelectric Point (pI)

40.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 843
AciI CCGC 2 cut(s) 73, 141
AclWI GGATC 2 cut(s) 214, 455
AcsI RAATTY 3 cut(s) 56, 480, 764
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 1 cut(s) 610
AgsI TTSAA 4 cut(s) 220, 355, 458, 511
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 7 cut(s) 213, 309, 569, 583, 639, 728, 777
AluI AGCT 7 cut(s) 213, 309, 569, 583, 639, 728, 777
Alw21I GWGCWC 1 cut(s) 331
Alw26I GTCTC 2 cut(s) 647, 813
AlwI GGATC 2 cut(s) 214, 455
AlwNI CAGNNNCTG 1 cut(s) 284
Aor13HI TCCGGA 1 cut(s) 843
AoxI GGCC 2 cut(s) 68, 848
ApeKI GCWGC 2 cut(s) 137, 788
ApoI RAATTY 3 cut(s) 56, 480, 764
AsiSI GCGATCGC 1 cut(s) 319
AspLEI GCGC 2 cut(s) 7, 450
AspS9I GGNCC 3 cut(s) 69, 401, 626
AsuHPI GGTGA 3 cut(s) 382, 560, 832
AvaII GGWCC 2 cut(s) 401, 626
Bbv12I GWGCWC 1 cut(s) 331
BbvI GCAGC 2 cut(s) 124, 800
BccI CCATC 4 cut(s) 29, 39, 500, 617
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 1 cut(s) 226
BcoDI GTCTC 2 cut(s) 647, 813
BfaI CTAG 3 cut(s) 528, 539, 677
BfmI CTRYAG 1 cut(s) 778
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 138, 141, 789
BlsI GCNGC 3 cut(s) 139, 142, 790
Bme1390I CCNGG 1 cut(s) 669
Bme18I GGWCC 2 cut(s) 401, 626
BmgT120I GGNCC 3 cut(s) 69, 401, 626
BmiI GGNNCC 2 cut(s) 71, 402
BmrFI CCNGG 1 cut(s) 669
BmrI ACTGGG 1 cut(s) 453
BmsI GCATC 2 cut(s) 277, 544
BmuI ACTGGG 1 cut(s) 453
BpmI CTGGAG 2 cut(s) 139, 555
Bpu10I CCTNAGC 1 cut(s) 378
Bsa29I ATCGAT 2 cut(s) 267, 714
BsaJI CCNNGG 3 cut(s) 65, 322, 668
BsaWI WCCGGW 1 cut(s) 843
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 3 cut(s) 254, 404, 448
Bse3DI GCAATG 2 cut(s) 549, 696
BseAI TCCGGA 1 cut(s) 843
BseBI CCWGG 1 cut(s) 669
BseCI ATCGAT 2 cut(s) 267, 714
BseDI CCNNGG 3 cut(s) 65, 322, 668
BseGI GGATG 3 cut(s) 376, 517, 840
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 2 cut(s) 549, 696
BseMII CTCAG 4 cut(s) 319, 387, 720, 824
BseNI ACTGG 3 cut(s) 254, 404, 448
BseRI GAGGAG 2 cut(s) 92, 115
BseXI GCAGC 2 cut(s) 124, 800
Bsh1285I CGRYCG 1 cut(s) 319
BshFI GGCC 2 cut(s) 70, 850
BshVI ATCGAT 2 cut(s) 267, 714
BsiEI CGRYCG 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 1 cut(s) 844
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 647, 813
BsmFI GGGAC 1 cut(s) 414
BsmI GAATGC 2 cut(s) 202, 352
BsnI GGCC 2 cut(s) 70, 850
Bsp1286I GDGCHC 1 cut(s) 331
Bsp13I TCCGGA 1 cut(s) 843
Bsp143I GATC 6 cut(s) 206, 226, 264, 268, 316, 460
BspACI CCGC 2 cut(s) 73, 141
BspANI GGCC 2 cut(s) 70, 850
BspCNI CTCAG 4 cut(s) 318, 388, 721, 823
BspDI ATCGAT 2 cut(s) 267, 714
BspEI TCCGGA 1 cut(s) 843
BspLI GGNNCC 2 cut(s) 71, 402
BspPI GGATC 2 cut(s) 214, 455
BspQI GCTCTTC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 549, 696
BsrI ACTGG 3 cut(s) 254, 404, 448
BssECI CCNNGG 3 cut(s) 65, 322, 668
BssMI GATC 6 cut(s) 206, 226, 264, 268, 316, 460
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 3 cut(s) 388, 800, 827
Bst6I CTCTTC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 177, 641
BstDEI CTNAG 6 cut(s) 305, 378, 396, 630, 729, 810
BstF5I GGATG 3 cut(s) 376, 517, 840
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 450
BstKTI GATC 6 cut(s) 209, 229, 267, 271, 319, 463
BstMAI GTCTC 2 cut(s) 647, 813
BstMBI GATC 6 cut(s) 206, 226, 264, 268, 316, 460
BstMCI CGRYCG 1 cut(s) 319
BstMWI GCNNNNNNNGC 2 cut(s) 11, 146
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 1 cut(s) 614
BstSCI CCNGG 1 cut(s) 667
BstSFI CTRYAG 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 124, 800
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
Bsu15I ATCGAT 2 cut(s) 267, 714
BsuRI GGCC 2 cut(s) 70, 850
BsuTUI ATCGAT 2 cut(s) 267, 714
BtsCI GGATG 3 cut(s) 376, 517, 840
BtsIMutI CAGTG 2 cut(s) 393, 441
Cac8I GCNNGC 2 cut(s) 177, 641
CaiI CAGNNNCTG 1 cut(s) 284
CfoI GCGC 2 cut(s) 7, 450
Cfr13I GGNCC 3 cut(s) 69, 401, 626
ClaI ATCGAT 2 cut(s) 267, 714
CseI GACGC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 85
CviAII CATG 2 cut(s) 611, 770
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 6 cut(s) 305, 378, 396, 630, 729, 810
DpnI GATC 6 cut(s) 208, 228, 266, 270, 318, 462
DpnII GATC 6 cut(s) 206, 226, 264, 268, 316, 460
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 401, 626
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 2 cut(s) 614, 773
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FaqI GGGAC 1 cut(s) 414
FatI CATG 2 cut(s) 610, 769
FauI CCCGC 1 cut(s) 80
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 3 cut(s) 138, 141, 789
FokI GGATG 3 cut(s) 383, 504, 827
Fsp4HI GCNGC 3 cut(s) 138, 141, 789
FspBI CTAG 3 cut(s) 528, 539, 677
GlaI GCGC 2 cut(s) 6, 449
GluI GCNGC 3 cut(s) 138, 141, 789
GsuI CTGGAG 2 cut(s) 139, 555
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 2 cut(s) 70, 850
HapII CCGG 1 cut(s) 844
HgaI GACGC 1 cut(s) 168
HhaI GCGC 2 cut(s) 7, 450
Hin1II CATG 2 cut(s) 614, 773
Hin6I GCGC 2 cut(s) 5, 448
HinP1I GCGC 2 cut(s) 5, 448
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 4 cut(s) 275, 281, 524, 722
HpaII CCGG 1 cut(s) 844
HphI GGTGA 3 cut(s) 382, 560, 832
Hpy166II GTNNAC 1 cut(s) 823
Hpy188I TCNGA 5 cut(s) 55, 195, 226, 280, 286
Hpy188III TCNNGA 6 cut(s) 156, 272, 464, 521, 572, 844
Hpy8I GTNNAC 1 cut(s) 823
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 2 cut(s) 610, 737
HpyCH4III ACNGT 3 cut(s) 388, 800, 827
HpyCH4IV ACGT 2 cut(s) 133, 705
HpyCH4V TGCA 5 cut(s) 200, 290, 301, 350, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 146
HpyF3I CTNAG 6 cut(s) 305, 378, 396, 630, 729, 810
HpySE526I ACGT 2 cut(s) 133, 705
Hsp92II CATG 2 cut(s) 614, 773
HspAI GCGC 2 cut(s) 5, 448
Kpn2I TCCGGA 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 6 cut(s) 206, 226, 264, 268, 316, 460
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 3 cut(s) 128, 326, 574
Lsp1109I GCAGC 2 cut(s) 124, 800
LweI GCATC 2 cut(s) 277, 544
MaeI CTAG 3 cut(s) 528, 539, 677
MaeII ACGT 2 cut(s) 133, 705
MaeIII GTNAC 3 cut(s) 388, 833, 869
MalI GATC 6 cut(s) 208, 228, 266, 270, 318, 462
MboI GATC 6 cut(s) 206, 226, 264, 268, 316, 460
MboII GAAGA 6 cut(s) 9, 236, 470, 589, 632, 646
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 3 cut(s) 56, 480, 764
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 7 cut(s) 75, 88, 113, 136, 391, 776, 840
Mph1103I ATGCAT 1 cut(s) 352
MroI TCCGGA 1 cut(s) 843
MseI TTAA 2 cut(s) 708, 794
MslI CAYNNNNRTG 1 cut(s) 609
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 1 cut(s) 844
MspR9I CCNGG 1 cut(s) 669
Mva1269I GAATGC 2 cut(s) 202, 352
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 2 cut(s) 11, 146
NdeII GATC 6 cut(s) 206, 226, 264, 268, 316, 460
NlaIII CATG 2 cut(s) 614, 773
NlaIV GGNNCC 2 cut(s) 71, 402
NmeAIII GCCGAG 1 cut(s) 46
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 1 cut(s) 614
PciI ACATGT 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 629
PcsI WCGNNNNNNNCGW 1 cut(s) 711
PctI GAATGC 2 cut(s) 202, 352
PfeI GAWTC 4 cut(s) 275, 281, 524, 722
Pfl23II CGTACG 1 cut(s) 84
PkrI GCNGC 3 cut(s) 139, 142, 790
Ple19I CGATCG 1 cut(s) 319
PscI ACATGT 1 cut(s) 610
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 71, 402
PspPI GGNCC 3 cut(s) 69, 401, 626
PstNI CAGNNNCTG 1 cut(s) 284
PsuI RGATCY 1 cut(s) 460
PvuI CGATCG 1 cut(s) 319
PvuII CAGCTG 1 cut(s) 728
RgaI GCGATCGC 1 cut(s) 319
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 2 cut(s) 708, 794
SatI GCNGC 3 cut(s) 138, 141, 789
Sau3AI GATC 6 cut(s) 206, 226, 264, 268, 316, 460
Sau96I GGNCC 3 cut(s) 69, 401, 626
ScrFI CCNGG 1 cut(s) 669
SduI GDGCHC 1 cut(s) 331
SfaAI GCGATCGC 1 cut(s) 319
SfaNI GCATC 2 cut(s) 277, 544
SfcI CTRYAG 1 cut(s) 778
SgfI GCGATCGC 1 cut(s) 319
SinI GGWCC 2 cut(s) 401, 626
SmiMI CAYNNNNRTG 1 cut(s) 609
Sse9I AATT 3 cut(s) 56, 480, 764
SsiI CCGC 2 cut(s) 73, 141
SspMI CTAG 3 cut(s) 528, 539, 677
StyD4I CCNGG 1 cut(s) 667
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 3 cut(s) 388, 800, 827
TaiI ACGT 2 cut(s) 136, 708
TaqI TCGA 4 cut(s) 182, 267, 484, 714
TasI AATT 3 cut(s) 56, 480, 764
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 4 cut(s) 275, 281, 524, 722
Tru1I TTAA 2 cut(s) 708, 794
Tru9I TTAA 2 cut(s) 708, 794
TscAI CASTG 2 cut(s) 400, 448
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 2 cut(s) 137, 788
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 3 cut(s) 200, 359, 758
TspRI CASTG 2 cut(s) 400, 448
VpaK11BI GGWCC 2 cut(s) 401, 626
XapI RAATTY 3 cut(s) 56, 480, 764
XceI RCATGY 1 cut(s) 614
XspI CTAG 3 cut(s) 528, 539, 677
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.