Rorug01G0100400

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0014353
Physical Location & Seq
Forward (+)
16719417 .. 16726075
6659 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0100400.1

Sequence Viewer

Length: 3573 bp
ATGACCTCAAACCGGCCTCTTTTGATCCCGTCTCCAAGAACACCCAACACCCACGATCTCCCAACTCTACCTGTATTTCCCGATTCAGCAAAACCCAGTTCCGCCATGGATTCTGAAAACCCACAACCCACTTTGAATTCTTCTTCTTCGAGACGTAGCATTTCTTCCGTTCAATCCAAGCCGTCCATGCGGTATGGTTCCAGAGGAGCTGATTCCGAAGCCGCCTCGTCGCAGAAGGAAATGAACGATGAGGATGTCAGGATCATACACATCAATGATCTTGCCCACACCAATGAGAGGTTTGAGTTTTCTGGCAACTCTATAAGGACCACAAAGTACTCAGTTATCACTTTCTTGCCTAGGAATTTGTTTGAACAGTTTCATAGAGTAGCTTATATGTATTTTCTAGTGATTGCTGTGCTCAATCAGCTCCCCCAGCTGGCAGTTTTCGGCCGGGGAGTTTCCATTTTGCCATTAGCATTTGTGTTGTTAGTTACTGCTGTTAAGGATGCGTATGAGGACTTTAGGAGGCATCGATCCGACCGGATTGAGAACAATCGCCTTGCTTCGGTTTTTGTTAACAATCAGTTTCAGTTGAAGAAATGGAAGGATGTACAAGTTGGTGAAATCATCAAAATTCAAGCAAATGAGGGTATTCCTTGTGATATGGTTCTGCTCTCCACCAGTGACCCAACTGGTGTGGCTTATGTGCAGACTATCAATTTAGATGGGGAGTCAAATTTGAAGACCCGGTATGCGAAGCAAGAAACCCTCTCCAAGTTGCCTGAAAAGGACAGGATTACAGGGATGATCAAGTGTGAGAAACCCAACAGGAATATTTATGGATTTCAGGCATTCATGGAGGTTGATGGGAAAAGACTGTCATTAGGTCCCTCCAACATTGTGCTTCGAGGCTGTGAGCTCAAGAATACTCATTGGGCCCTCGGGGTGGCTGTATATACTGGTCGCGAGACCAAAGTTATGCTCAATAGCTCTGGAGCCCCATCCAAGAGGAGTCGTCTCGAGACCCGTATGAACCACGAGATCATCATGCTCTCACTGTTTCTGGTCGTCTTGTGTTCAGTTGTCTCTCTGTGTGCTGCTGTTTGGTTGAGGCGCGAAAGTGATAATTTGGACAACATACTTTTCTACAGGAAGAAGGACTACTCTGAAGATGAAGTCAAGGATTATAAGTATTATGGATGGGCATTAGAGATTGTTTTCACGTTCCTCATGTCTATCATCGTGTTCCAGATCATGATCCCCATCTCTTTGTACATTTCTATGGAGCTCGTCCGGGTGGGTCAGGCTTACTTCATGATACGAGATAGTCAAATGTATGATGAGGCTTCTAATGCAAGATTTCAGTGCAGAGCTTTGAATATAAATGAAGATTTAGGACAAATTAAGTATGTATTCTCCGACAAAACTGGTACACTTACTGAGAACAAAATGGAATTTCAATGTGCAAGCATTTGGGGAGTTGATTATAATGGTGGGAGAGGCAGTTTGGAAAAGGACCAATTGGTGCACAATGTTCAAATTGATGGAATTGTTTTGAGGCCAAAGATGAATGTAAAGGCTGACCCAGATCTTCTACGACTACTAAGAAGTGAAAAGGACACAAATGAAGGCAAACATGTGCACGAGTTCTTCCTCGCGTTGGCAGCTTGTAATACTATTGTGCCTCTTGTTACAGACTCGTCTGATCCAAATGTGAGATTGATTGATTACCAAGGGGAGTCTCCAGATGAACAGGCATTAGTTTATGCTGCTGCCGCCTATGGTTTTATGCTTATTGAAAGAACGTCTGGTCATATAACTATTGATATCCAAGGAGAAAGGCAAAGGTTCAGTGTTCTGGGTTTGCATGAATTTGATAGTGACCGGAAGAGAATGTCGGTGATATTGGGGTGCCCTGATAAAACTGTGAAAGTGTTTGTAAAAGGTGCTGACACTACCATGTTCAGTGTAACAGACAGAAGATTGAACTTGAACATAATACGCGCAACAGAAGCCCATATTCATGCTTACTCCTCTCTGGGTTTGAGAACACTTGTTGTGGGGATGAGAGAACTCACTGCTTCAGAATTTGAGCAGTGGCACTCTTCTTTTGAGGCAGCAAGCACTGCTTTAATAGGCAGGCCTGCTCTGCTTCGCAAGGTTGCTGGCAACATAGAGAACAATCTCATAATTTTAGGTGCCTCTGGTATTGAAGACAAACTGCAACTGGGGGTGCCTGAGGCCATAGACTCTCTAAGAACTGCAGGGGTTCAGGTGTGGGTTTTGACGGGGGACAAGCAAGAAACTGCCATATCAATTGGCTACTCATCAAAGCTTCTTACAAGGAGGATGACCCAGATTTTAATTAACAACAGCTCTAAGGAGTCATGTAGACGGAATTTAGAAGATGCGATACTCATGTCTAAGAAGCTCACAACCGTGTCTGGCAATGAATACAGTACCCGAGGAAGTTCTGGAGATGGTATAACTTCATTAGCTTTAATTATCGATGGTACCAGCCTTGTTTATATTCTTGACAGTGAACTTGAGGAAAAGCTATTTGAATTAGCAAATAGATGTTCTGTGGTGCTCTGTTGCCGAGTGGCTCCTCTGCAAAAAGCTGGAATTGTTGCCCTTGTAAAAAACAGAACTGCTGATATGACACTTGCCATTGGGGATGGTGCTAATGATGTTTCGATGATCCAAATGGCTGATGTTGGAGTTGGAATCAGTGGCCAAGAGGGTCGACAAGCTGTCATGGCTTCAGATTTTGCAATGGGGCAGTTCAGATTCTTAGTTCCCCTCTTATTGGTACATGGGCATTGGAATTACCAGCGAATGGGCTACATGATACTTTACAACTTTTATAGGAATGCTGTGTTTGTTCTTATTTTGTTTTGGTATGTGCTCTTTACTTGTTTTACTCTGACAACTGCAATCACGGAATGGAGCAGCATGTTGTATTCTATAATCTACACTGCATTACCTACAATAGTTGTTGGGGTTCTTGACAAGGACCTAAGTAGAATGACCCTTCTATCATATCCTCAGCTATATGGAGCTGGTCACAGACAAGAGTGCTATAACACAAAATTATTTTGGGTAACAATGGTTGACACATTGTGGCAAAGTGTGGCTGTCTTCTTTATCCCCCTTTTTGCATATTGGGGTAGCACCATTGATACCTCAAGTATAGGAGATCTTTGGACTCTTTCGGTGGTTATATTGGTTAATATACACTTGGCTATGGATATTATTCGGTGGAGTTGGATTACACATGCAGCTATTTGGGGATCTATCATTGCAACTTGGATTTGTGTCATTGTAATTGATGCTATACCTTATCTGGTTGGCTACTGGGCCATTTTTGATGTCATGAAGACAGGACCATTTTGGTTATGTTTGCTCGCGATCATCATAGCAGCACTGACTCCCCGTTTTTGTGTAAAGTTTCTGTGTCAGTACTATAGTCCTTGTGATGTTCAGATTGCAAGAGAGGCTGAGAAGTTTGGCAATTTAAGTCCTGTACAAATAGAAATGAATCCGATCATGGAACTCCCAAGGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1190

Amino Acids

133.89

Weight (kDa)

6.73

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhoLip_ATPase_N PF16209 89 - 155 6.4e-25 Phospholipid-translocating ATPase N-terminal
Cation_ATPase PF13246 552 - 743 4.2e-14 P-type ATPase, cytoplasmic domain N
PhoLip_ATPase_C PF16212 920 - 1159 2.9e-74 Phospholipid-translocating P-type ATPase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1191, 1491
Acc65I GGTACC 1 cut(s) 2516
AccB1I GGYRCC 4 cut(s) 1914, 2201, 2236, 2516
AccB7I CCANNNNNTGG 1 cut(s) 2842
AccI GTMKAC 2 cut(s) 2395, 2749
AccII CGCG 5 cut(s) 969, 1119, 1661, 2007, 3413
AciI CCGC 4 cut(s) 102, 190, 222, 1779
AclWI GGATC 7 cut(s) 19, 269, 531, 1255, 1703, 2698, 3304
AcoI YGGCCR 2 cut(s) 451, 2737
AcsI RAATTY 8 cut(s) 136, 364, 636, 739, 1457, 1874, 2090, 2401
AcuI CTGAAG 3 cut(s) 1191, 2070, 2751
AdeI CACNNNGTG 1 cut(s) 3126
AfaI GTAC 9 cut(s) 338, 615, 1277, 1435, 2464, 2518, 2817, 3467, 3531
AfiI CCNNNNNNNGG 8 cut(s) 12, 297, 439, 568, 949, 1663, 2812, 2842
AflIII ACRYGT 1 cut(s) 1639
AhdI GACNNNNNGTC 1 cut(s) 2756
AleI CACNNNNGTG 1 cut(s) 2441
Alw21I GWGCWC 7 cut(s) 423, 924, 1293, 1533, 1647, 2595, 2913
Alw26I GTCTC 7 cut(s) 36, 145, 965, 1019, 1025, 1093, 1749
Alw44I GTGCAC 2 cut(s) 1529, 1643
AlwI GGATC 7 cut(s) 19, 269, 531, 1255, 1703, 2698, 3304
Ama87I CYCGRG 3 cut(s) 944, 1022, 2466
AoxI GGCC 8 cut(s) 14, 451, 939, 1562, 2144, 2244, 2737, 3363
ApaI GGGCCC 1 cut(s) 943
ApaLI GTGCAC 2 cut(s) 1529, 1643
ApeKI GCWGC 8 cut(s) 1100, 1667, 1772, 1775, 2120, 2955, 3284, 3425
ApoI RAATTY 8 cut(s) 136, 364, 636, 739, 1457, 1874, 2090, 2401
Asp700I GAANNNNTTC 1 cut(s) 378
Asp718I GGTACC 1 cut(s) 2516
AspA2I CCTAGG 1 cut(s) 359
AspLEI GCGC 2 cut(s) 1119, 2009
AspS9I GGNCC 8 cut(s) 327, 890, 939, 940, 1519, 3019, 3363, 3389
AsuC2I CCSGG 3 cut(s) 455, 751, 1298
AsuHPI GGTGA 2 cut(s) 635, 1915
AvaI CYCGRG 3 cut(s) 944, 1022, 2466
AvaII GGWCC 5 cut(s) 327, 890, 1519, 3019, 3389
AvrII CCTAGG 1 cut(s) 359
AxyI CCTNAGG 1 cut(s) 2241
BaeGI GKGCMC 4 cut(s) 943, 1533, 1647, 1919
BaeI ACNNNNGTAYC 2 cut(s) 2408, 2441
BalI TGGCCA 1 cut(s) 2739
BanI GGYRCC 4 cut(s) 1914, 2201, 2236, 2516
BanII GRGCYC 4 cut(s) 924, 943, 1003, 1293
BarI GAAGNNNNNNTAC 6 cut(s) 148, 180, 1148, 1180, 2400, 2432
BauI CACGAG 2 cut(s) 1040, 1646
BbsI GAAGAC 4 cut(s) 752, 2223, 3136, 3389
Bbv12I GWGCWC 7 cut(s) 423, 924, 1293, 1533, 1647, 2595, 2913
BbvCI CCTCAGC 1 cut(s) 3051
BbvI GCAGC 8 cut(s) 1087, 1679, 1759, 1762, 2132, 2967, 3296, 3437
BccI CCATC 9 cut(s) 722, 863, 1012, 1197, 1274, 1541, 2477, 2507, 2675
BceAI ACGGC 1 cut(s) 166
BclI TGATCA 1 cut(s) 810
BcnI CCSGG 3 cut(s) 455, 751, 1298
BcoDI GTCTC 7 cut(s) 36, 145, 965, 1019, 1025, 1093, 1749
BfaI CTAG 2 cut(s) 360, 407
BfmI CTRYAG 3 cut(s) 1150, 2265, 3469
BglII AGATCT 2 cut(s) 1591, 3202
BlnI CCTAGG 1 cut(s) 359
BmcAI AGTACT 2 cut(s) 338, 3467
Bme1390I CCNGG 3 cut(s) 455, 751, 1298
Bme18I GGWCC 5 cut(s) 327, 890, 1519, 3019, 3389
BmeRI GACNNNNNGTC 1 cut(s) 2756
BmeT110I CYCGRG 3 cut(s) 944, 1022, 2466
BmgT120I GGNCC 8 cut(s) 327, 890, 939, 940, 1519, 3019, 3363, 3389
BmiI GGNNCC 9 cut(s) 199, 892, 941, 1000, 1916, 2203, 2238, 2518, 2610
BmrFI CCNGG 3 cut(s) 455, 751, 1298
BmrI ACTGGG 3 cut(s) 90, 2240, 3370
BmsI GCATC 4 cut(s) 499, 541, 2401, 3325
BmuI ACTGGG 3 cut(s) 90, 2240, 3370
BpiI GAAGAC 4 cut(s) 752, 2223, 3136, 3389
BpmI CTGGAG 3 cut(s) 1017, 1731, 2499
Bpu10I CCTNAGC 1 cut(s) 3051
BpuEI CTTGAG 3 cut(s) 908, 2570, 3175
BpuMI CCSGG 3 cut(s) 455, 751, 1298
Bsa29I ATCGAT 2 cut(s) 535, 2511
BsaBI GATNNNNATC 2 cut(s) 1259, 1265
BsaI GGTCTC 2 cut(s) 965, 1019
BsaJI CCNNGG 8 cut(s) 105, 359, 454, 943, 1735, 1834, 2467, 3563
BsaWI WCCGGW 2 cut(s) 543, 1887
BsaXI ACNNNNNCTCC 2 cut(s) 1492, 1522
Bsc4I CCNNNNNNNGG 8 cut(s) 12, 297, 439, 568, 949, 1663, 2812, 2842
Bse118I RCCGGY 1 cut(s) 12
Bse1I ACTGG 7 cut(s) 96, 684, 700, 967, 1435, 2235, 3365
Bse21I CCTNAGG 1 cut(s) 2241
Bse3DI GCAATG 3 cut(s) 2458, 2784, 3303
Bse8I GATNNNNATC 2 cut(s) 1259, 1265
BseCI ATCGAT 2 cut(s) 535, 2511
BseDI CCNNGG 8 cut(s) 105, 359, 454, 943, 1735, 1834, 2467, 3563
BseGI GGATG 9 cut(s) 259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BseJI GATNNNNATC 2 cut(s) 1259, 1265
BseLI CCNNNNNNNGG 8 cut(s) 12, 297, 439, 568, 949, 1663, 2812, 2842
BseMI GCAATG 3 cut(s) 2458, 2784, 3303
BseMII CTCAG 5 cut(s) 354, 1434, 2232, 3065, 3495
BseNI ACTGG 7 cut(s) 96, 684, 700, 967, 1435, 2235, 3365
BseRI GAGGAG 4 cut(s) 219, 1027, 2026, 2601
BseSI GKGCMC 4 cut(s) 943, 1533, 1647, 1919
BseX3I CGGCCG 1 cut(s) 451
BseXI GCAGC 8 cut(s) 1087, 1679, 1759, 1762, 2132, 2967, 3296, 3437
BseYI CCCAGC 1 cut(s) 435
BsgI GTGCAG 2 cut(s) 731, 1390
Bsh1236I CGCG 5 cut(s) 969, 1119, 1661, 2007, 3413
Bsh1285I CGRYCG 2 cut(s) 454, 544
BshFI GGCC 8 cut(s) 16, 453, 941, 1564, 2146, 2246, 2739, 3365
BshNI GGYRCC 4 cut(s) 1914, 2201, 2236, 2516
BshVI ATCGAT 2 cut(s) 535, 2511
BsiEI CGRYCG 2 cut(s) 454, 544
BsiHKAI GWGCWC 7 cut(s) 423, 924, 1293, 1533, 1647, 2595, 2913
BsiHKCI CYCGRG 3 cut(s) 944, 1022, 2466
BsiSI CCGG 6 cut(s) 13, 454, 544, 751, 1297, 1888
BslFI GGGAC 2 cut(s) 876, 2309
BslI CCNNNNNNNGG 8 cut(s) 12, 297, 439, 568, 949, 1663, 2812, 2842
BsmAI GTCTC 7 cut(s) 36, 145, 965, 1019, 1025, 1093, 1749
BsmBI CGTCTC 3 cut(s) 36, 145, 1025
BsmFI GGGAC 2 cut(s) 876, 2309
BsmI GAATGC 2 cut(s) 854, 2881
BsnI GGCC 8 cut(s) 16, 453, 941, 1564, 2146, 2246, 2739, 3365
Bso31I GGTCTC 2 cut(s) 965, 1019
BsoBI CYCGRG 3 cut(s) 944, 1022, 2466
Bsp120I GGGCCC 1 cut(s) 939
Bsp1407I TGTACA 3 cut(s) 613, 1275, 3529
Bsp19I CCATGG 1 cut(s) 105
Bsp68I TCGCGA 2 cut(s) 969, 3413
BspACI CCGC 4 cut(s) 102, 190, 222, 1779
BspANI GGCC 8 cut(s) 16, 453, 941, 1564, 2146, 2246, 2739, 3365
BspCNI CTCAG 5 cut(s) 353, 1435, 2233, 3064, 3496
BspDI ATCGAT 2 cut(s) 535, 2511
BspFNI CGCG 5 cut(s) 969, 1119, 1661, 2007, 3413
BspHI TCATGA 3 cut(s) 1257, 1317, 3378
BspLI GGNNCC 9 cut(s) 199, 892, 941, 1000, 1916, 2203, 2238, 2518, 2610
BspMAI CTGCAG 1 cut(s) 2269
BspPI GGATC 7 cut(s) 19, 269, 531, 1255, 1703, 2698, 3304
BspT107I GGYRCC 4 cut(s) 1914, 2201, 2236, 2516
BspTNI GGTCTC 2 cut(s) 965, 1019
BsrDI GCAATG 3 cut(s) 2458, 2784, 3303
BsrFI RCCGGY 1 cut(s) 12
BsrGI TGTACA 3 cut(s) 613, 1275, 3529
BsrI ACTGG 7 cut(s) 96, 684, 700, 967, 1435, 2235, 3365
BssAI RCCGGY 1 cut(s) 12
BssECI CCNNGG 8 cut(s) 105, 359, 454, 943, 1735, 1834, 2467, 3563
BssSI CACGAG 2 cut(s) 1040, 1646
BssT1I CCWWGG 5 cut(s) 105, 359, 1735, 1834, 3563
Bst2BI CACGAG 2 cut(s) 1040, 1646
Bst4CI ACNGT 7 cut(s) 378, 882, 1062, 1930, 2443, 2462, 2543
Bst6I CTCTTC 2 cut(s) 1886, 2113
BstAPI GCANNNNNTGC 1 cut(s) 2129
BstAUI TGTACA 3 cut(s) 613, 1275, 3529
BstC8I GCNNGC 7 cut(s) 441, 1471, 2125, 2144, 2148, 2170, 3411
BstDSI CCRYGG 1 cut(s) 105
BstF5I GGATG 9 cut(s) 259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BstFNI CGCG 5 cut(s) 969, 1119, 1661, 2007, 3413
BstHHI GCGC 2 cut(s) 1119, 2009
BstMAI GTCTC 7 cut(s) 36, 145, 965, 1019, 1025, 1093, 1749
BstMCI CGRYCG 2 cut(s) 454, 544
BstNSI RCATGY 3 cut(s) 1643, 2962, 3284
BstSCI CCNGG 3 cut(s) 453, 749, 1296
BstSFI CTRYAG 3 cut(s) 1150, 2265, 3469
BstSLI GKGCMC 4 cut(s) 943, 1533, 1647, 1919
BstUI CGCG 5 cut(s) 969, 1119, 1661, 2007, 3413
BstV1I GCAGC 8 cut(s) 1087, 1679, 1759, 1762, 2132, 2967, 3296, 3437
BstV2I GAAGAC 4 cut(s) 752, 2223, 3136, 3389
BstX2I RGATCY 3 cut(s) 1591, 3202, 3296
BstYI RGATCY 3 cut(s) 1591, 3202, 3296
BstZI CGGCCG 1 cut(s) 451
Bsu15I ATCGAT 2 cut(s) 535, 2511
Bsu36I CCTNAGG 1 cut(s) 2241
BsuRI GGCC 8 cut(s) 16, 453, 941, 1564, 2146, 2246, 2739, 3365
BsuTUI ATCGAT 2 cut(s) 535, 2511
BtgI CCRYGG 1 cut(s) 105
BtsCI GGATG 9 cut(s) 259, 514, 616, 813, 1004, 1208, 2073, 2358, 2686
BtsI GCAGTG 4 cut(s) 2079, 2105, 2127, 2979
BtuMI TCGCGA 2 cut(s) 969, 3413
Cac8I GCNNGC 7 cut(s) 441, 1471, 2125, 2144, 2148, 2170, 3411
CciI TCATGA 3 cut(s) 1257, 1317, 3378
CfoI GCGC 2 cut(s) 1119, 2009
Cfr10I RCCGGY 1 cut(s) 12
Cfr13I GGNCC 8 cut(s) 327, 890, 939, 940, 1519, 3019, 3363, 3389
ClaI ATCGAT 2 cut(s) 535, 2511
Csp6I GTAC 9 cut(s) 337, 614, 1276, 1434, 2463, 2517, 2816, 3466, 3530
CspCI CAANNNNNGTGG 2 cut(s) 681, 716
CviQI GTAC 9 cut(s) 337, 614, 1276, 1434, 2463, 2517, 2816, 3466, 3530
DraIII CACNNNGTG 1 cut(s) 3126
DriI GACNNNNNGTC 1 cut(s) 2756
EaeI YGGCCR 2 cut(s) 451, 2737
EagI CGGCCG 1 cut(s) 451
Eam1104I CTCTTC 2 cut(s) 1886, 2113
Eam1105I GACNNNNNGTC 1 cut(s) 2756
EarI CTCTTC 2 cut(s) 1886, 2113
EciI GGCGGA 1 cut(s) 91
Ecl136II GAGCTC 2 cut(s) 922, 1291
EclXI CGGCCG 1 cut(s) 451
Eco130I CCWWGG 5 cut(s) 105, 359, 1735, 1834, 3563
Eco147I AGGCCT 1 cut(s) 2146
Eco24I GRGCYC 4 cut(s) 924, 943, 1003, 1293
Eco31I GGTCTC 2 cut(s) 965, 1019
Eco32I GATATC 1 cut(s) 1831
Eco47I GGWCC 5 cut(s) 327, 890, 1519, 3019, 3389
Eco52I CGGCCG 1 cut(s) 451
Eco53kI GAGCTC 2 cut(s) 922, 1291
Eco57I CTGAAG 3 cut(s) 1191, 2070, 2751
Eco81I CCTNAGG 1 cut(s) 2241
Eco88I CYCGRG 3 cut(s) 944, 1022, 2466
EcoICRI GAGCTC 2 cut(s) 922, 1291
EcoO109I RGGNCCY 3 cut(s) 890, 940, 3019
EcoRI GAATTC 1 cut(s) 136
EcoRV GATATC 1 cut(s) 1831
EcoT14I CCWWGG 5 cut(s) 105, 359, 1735, 1834, 3563
EcoT38I GRGCYC 4 cut(s) 924, 943, 1003, 1293
ErhI CCWWGG 5 cut(s) 105, 359, 1735, 1834, 3563
Esp3I CGTCTC 3 cut(s) 36, 145, 1025
FalI AAGNNNNNCTT 2 cut(s) 2116, 2148
FaqI GGGAC 2 cut(s) 876, 2309
FbaI TGATCA 1 cut(s) 810
FblI GTMKAC 2 cut(s) 2395, 2749
FokI GGATG 9 cut(s) 266, 521, 623, 820, 991, 1215, 2080, 2365, 2693
FriOI GRGCYC 4 cut(s) 924, 943, 1003, 1293
FspBI CTAG 2 cut(s) 360, 407
GlaI GCGC 2 cut(s) 1118, 2008
GsaI CCCAGC 1 cut(s) 439
GsuI CTGGAG 3 cut(s) 1017, 1731, 2499
HaeIII GGCC 8 cut(s) 16, 453, 941, 1564, 2146, 2246, 2739, 3365
HapII CCGG 6 cut(s) 13, 454, 544, 751, 1297, 1888
HhaI GCGC 2 cut(s) 1119, 2009
Hin6I GCGC 2 cut(s) 1117, 2007
HinP1I GCGC 2 cut(s) 1117, 2007
HincII GTYRAC 3 cut(s) 580, 2750, 3118
HindII GTYRAC 3 cut(s) 580, 2750, 3118
HindIII AAGCTT 1 cut(s) 2336
HpaI GTTAAC 1 cut(s) 580
HpaII CCGG 6 cut(s) 13, 454, 544, 751, 1297, 1888
HphI GGTGA 2 cut(s) 635, 1915
Hpy166II GTNNAC 8 cut(s) 580, 1436, 1531, 1645, 2396, 2546, 2750, 3118
Hpy8I GTNNAC 8 cut(s) 580, 1436, 1531, 1645, 2396, 2546, 2750, 3118
Hpy99I CGWCG 1 cut(s) 232
HpyAV CCTTC 5 cut(s) 229, 601, 1153, 1625, 3047
HpyCH4III ACNGT 7 cut(s) 378, 882, 1062, 1930, 2443, 2462, 2543
HpyCH4IV ACGT 3 cut(s) 154, 1226, 1808
HpySE526I ACGT 3 cut(s) 154, 1226, 1808
HspAI GCGC 2 cut(s) 1117, 2007
KpnI GGTACC 1 cut(s) 2520
Ksp22I TGATCA 1 cut(s) 810
KspAI GTTAAC 1 cut(s) 580
LmnI GCTCC 7 cut(s) 206, 435, 998, 1288, 2614, 2952, 3062
Lsp1109I GCAGC 8 cut(s) 1087, 1679, 1759, 1762, 2132, 2967, 3296, 3437
LweI GCATC 4 cut(s) 499, 541, 2401, 3325
MaeI CTAG 2 cut(s) 360, 407
MaeII ACGT 3 cut(s) 154, 1226, 1808
MaeIII GTNAC 7 cut(s) 493, 686, 1693, 1883, 1972, 3068, 3106
MfeI CAATTG 2 cut(s) 1523, 2319
MflI RGATCY 3 cut(s) 1591, 3202, 3296
MlsI TGGCCA 1 cut(s) 2739
MluNI TGGCCA 1 cut(s) 2739
MlyI GAGTC 8 cut(s) 743, 1024, 1694, 1751, 2246, 2396, 3205, 3427
MmeI TCCRAC 6 cut(s) 564, 921, 1446, 2701, 2707, 3251
Mox20I TGGCCA 1 cut(s) 2739
MroXI GAANNNNTTC 1 cut(s) 378
MscI TGGCCA 1 cut(s) 2739
MseI TTAA 9 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234, 3521
MslI CAYNNNNRTG 6 cut(s) 273, 291, 1283, 1961, 2025, 2441
Msp20I TGGCCA 1 cut(s) 2739
MspA1I CMGCKG 1 cut(s) 439
MspI CCGG 6 cut(s) 13, 454, 544, 751, 1297, 1888
MspR9I CCNGG 3 cut(s) 455, 751, 1298
MunI CAATTG 2 cut(s) 1523, 2319
Mva1269I GAATGC 2 cut(s) 854, 2881
MvnI CGCG 5 cut(s) 969, 1119, 1661, 2007, 3413
NciI CCSGG 3 cut(s) 455, 751, 1298
NcoI CCATGG 1 cut(s) 105
NlaIV GGNNCC 9 cut(s) 199, 892, 941, 1000, 1916, 2203, 2238, 2518, 2610
NmeAIII GCCGAG 1 cut(s) 2627
NmuCI GTSAC 3 cut(s) 686, 1883, 3068
NruI TCGCGA 2 cut(s) 969, 3413
NspI RCATGY 3 cut(s) 1643, 2962, 3284
OliI CACNNNNGTG 1 cut(s) 2441
PacI TTAATTAA 1 cut(s) 2370
PaeR7I CTCGAG 1 cut(s) 1022
PagI TCATGA 3 cut(s) 1257, 1317, 3378
PceI AGGCCT 1 cut(s) 2146
PciI ACATGT 1 cut(s) 1639
PctI GAATGC 2 cut(s) 854, 2881
PdmI GAANNNNTTC 1 cut(s) 378
PfeI GAWTC 6 cut(s) 83, 110, 212, 2730, 2793, 3544
PflMI CCANNNNNTGG 1 cut(s) 2842
PleI GAGTC 8 cut(s) 742, 1023, 1694, 1750, 2246, 2395, 3205, 3427
PpsI GAGTC 8 cut(s) 742, 1023, 1694, 1750, 2246, 2395, 3205, 3427
PpuMI RGGWCCY 2 cut(s) 890, 3019
PscI ACATGT 1 cut(s) 1639
PsiI TTATAA 2 cut(s) 1191, 1491
Psp124BI GAGCTC 2 cut(s) 924, 1293
Psp5II RGGWCCY 2 cut(s) 890, 3019
PspFI CCCAGC 1 cut(s) 435
PspN4I GGNNCC 9 cut(s) 199, 892, 941, 1000, 1916, 2203, 2238, 2518, 2610
PspOMI GGGCCC 1 cut(s) 939
PspPI GGNCC 8 cut(s) 327, 890, 939, 940, 1519, 3019, 3363, 3389
PspPPI RGGWCCY 2 cut(s) 890, 3019
PstI CTGCAG 1 cut(s) 2269
PsuI RGATCY 3 cut(s) 1591, 3202, 3296
PvuII CAGCTG 1 cut(s) 439
RruI TCGCGA 2 cut(s) 969, 3413
RsaI GTAC 9 cut(s) 338, 615, 1277, 1435, 2464, 2518, 2817, 3467, 3531
RsaNI GTAC 9 cut(s) 337, 614, 1276, 1434, 2463, 2517, 2816, 3466, 3530
RseI CAYNNNNRTG 6 cut(s) 273, 291, 1283, 1961, 2025, 2441
SacI GAGCTC 2 cut(s) 924, 1293
SalI GTCGAC 1 cut(s) 2748
SaqAI TTAA 9 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234, 3521
Sau96I GGNCC 8 cut(s) 327, 890, 939, 940, 1519, 3019, 3363, 3389
ScaI AGTACT 2 cut(s) 338, 3467
SchI GAGTC 8 cut(s) 743, 1024, 1694, 1751, 2246, 2396, 3205, 3427
ScrFI CCNGG 3 cut(s) 455, 751, 1298
SfaNI GCATC 4 cut(s) 499, 541, 2401, 3325
SfcI CTRYAG 3 cut(s) 1150, 2265, 3469
Sfr274I CTCGAG 1 cut(s) 1022
SinI GGWCC 5 cut(s) 327, 890, 1519, 3019, 3389
SlaI CTCGAG 1 cut(s) 1022
SmiMI CAYNNNNRTG 6 cut(s) 273, 291, 1283, 1961, 2025, 2441
SmlI CTYRAG 4 cut(s) 923, 1022, 2549, 3190
SmoI CTYRAG 4 cut(s) 923, 1022, 2549, 3190
SseBI AGGCCT 1 cut(s) 2146
SsiI CCGC 4 cut(s) 102, 190, 222, 1779
SspI AATATT 1 cut(s) 838
SspMI CTAG 2 cut(s) 360, 407
SstI GAGCTC 2 cut(s) 924, 1293
StuI AGGCCT 1 cut(s) 2146
StyD4I CCNGG 3 cut(s) 453, 749, 1296
StyI CCWWGG 5 cut(s) 105, 359, 1735, 1834, 3563
TaaI ACNGT 7 cut(s) 378, 882, 1062, 1930, 2443, 2462, 2543
TaiI ACGT 3 cut(s) 157, 1229, 1811
TaqI TCGA 7 cut(s) 149, 535, 910, 1023, 2511, 2699, 2749
TatI WGTACW 5 cut(s) 336, 613, 1275, 3465, 3529
TauI GCSGC 2 cut(s) 224, 1781
TfiI GAWTC 6 cut(s) 83, 110, 212, 2730, 2793, 3544
Tru1I TTAA 9 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234, 3521
Tru9I TTAA 9 cut(s) 504, 579, 1407, 2135, 2366, 2370, 2504, 3234, 3521
TseFI GTSAC 3 cut(s) 686, 1883, 3068
TseI GCWGC 8 cut(s) 1100, 1667, 1772, 1775, 2120, 2955, 3284, 3425
Tsp45I GTSAC 3 cut(s) 686, 1883, 3068
TspGWI ACGGA 3 cut(s) 157, 2413, 2960
Van91I CCANNNNNTGG 1 cut(s) 2842
VneI GTGCAC 2 cut(s) 1529, 1643
VpaK11BI GGWCC 5 cut(s) 327, 890, 1519, 3019, 3389
XapI RAATTY 8 cut(s) 136, 364, 636, 739, 1457, 1874, 2090, 2401
XceI RCATGY 3 cut(s) 1643, 2962, 3284
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XhoI CTCGAG 1 cut(s) 1022
XmaJI CCTAGG 1 cut(s) 359
XmiI GTMKAC 2 cut(s) 2395, 2749
XmnI GAANNNNTTC 1 cut(s) 378
XspI CTAG 2 cut(s) 360, 407
ZrmI AGTACT 2 cut(s) 338, 3467
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.