Rroxscaffold_4G00318220

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014353
Physical Location & Seq
Reverse (-)
44268795 .. 44276023
7229 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318220.1

Sequence Viewer

Length: 870 bp
ATGGCCTTCACCAACTCCTCTCTCTTTGCTTCCCATCTCACTCTCCTCCGCTCTAAACTTTCCGATTCCGAATTTGCCCCTCCGCCATGCGACGCCCTCAAGCTCCTTCACTTTCCTCCCAACGCCATTCATGCCAGCAGTTTCTCCAGAAATTTGAACGTTGTGTGTGCATCTACCAATGGTGTCAATGCAGTTGGTTCATCGTCGCCGCGGAAGTCTGATCAAGATGCTGATTATATTCCAATGCCAGTTGTCATGATTGATCAAGATTCAGATTCTCATGCAACTATTGTGCAACTCAGCTTTGGTGATCGCCTTGGAGCTCTTATTGACACGATAAATGCATTAAAAGATTTGGGATTGGATGTAGCAAAGGGAACTGTTATCACTGAGGGACCAGTTAAACAGACGAAGTTTCATATAACGCGGTTAGACACTGGGCGCAAAGTTGAAGATCCTGATATGTTAGAGAGAATTCGACTTACCATCATCAACAATCTTTTGAAGTATCATCCAGAATCTAGTCAGCTACTTGCAATGGGTGAAGCTTTTGGAATAAAAGCTCCAGAAAAGAAGCTTGATGTTGATATCGCAACTCATATACACGTGAAGCAAGATGGACCTAAGAGGAGCTTGCTTTATATAGAGACAGCAGATCGACCAGGATTGCTTGTAGAAATTATCAAAATCATTGCTGACATTAATATCGATGTGGAATCAGCTGAGATTGATACAGAAGGTTTGGTTGCTAAAGATAAATTTCATGTCAGCTACAGAGGGGCAGCCTTAAATAGTTCCTTGTCCCAGGTGTTAGTGAACTGTCTGCGTTACTATATTCGCAGGCCAGAAACAGATATAGATAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

289

Amino Acids

31.76

Weight (kDa)

5.71

Isoelectric Point (pI)

42.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 51
AccII CGCG 2 cut(s) 211, 427
AciI CCGC 5 cut(s) 49, 83, 209, 211, 427
AclI AACGTT 1 cut(s) 159
AclWI GGATC 1 cut(s) 449
AcsI RAATTY 4 cut(s) 71, 151, 474, 758
AcvI CACGTG 1 cut(s) 607
AcyI GRCGYC 1 cut(s) 93
AflIII ACRYGT 1 cut(s) 604
AgsI TTSAA 3 cut(s) 157, 452, 505
AjnI CCWGG 2 cut(s) 661, 804
Alw21I GWGCWC 1 cut(s) 325
Alw26I GTCTC 1 cut(s) 641
AlwI GGATC 1 cut(s) 449
AoxI GGCC 2 cut(s) 3, 842
ApeKI GCWGC 1 cut(s) 782
ApoI RAATTY 4 cut(s) 71, 151, 474, 758
AseI ATTAAT 1 cut(s) 702
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 2 cut(s) 395, 620
AsuHPI GGTGA 2 cut(s) 320, 554
AvaII GGWCC 2 cut(s) 395, 620
BanII GRGCYC 1 cut(s) 325
BbrPI CACGTG 1 cut(s) 607
Bbv12I GWGCWC 1 cut(s) 325
BbvI GCAGC 1 cut(s) 794
BccI CCATC 3 cut(s) 42, 494, 611
BciT130I CCWGG 2 cut(s) 663, 806
BclI TGATCA 2 cut(s) 220, 262
BcoDI GTCTC 1 cut(s) 641
BfaI CTAG 1 cut(s) 522
BfmI CTRYAG 1 cut(s) 772
BisI GCNGC 2 cut(s) 209, 783
BlsI GCNGC 2 cut(s) 210, 784
Bme1390I CCNGG 2 cut(s) 663, 806
Bme18I GGWCC 2 cut(s) 395, 620
BmgT120I GGNCC 2 cut(s) 395, 620
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 663, 806
BmrI ACTGGG 1 cut(s) 447
BmsI GCATC 2 cut(s) 179, 217
BmuI ACTGGG 1 cut(s) 447
BpmI CTGGAG 2 cut(s) 130, 549
BpuEI CTTGAG 1 cut(s) 83
Bsa29I ATCGAT 1 cut(s) 708
BsaAI YACGTR 1 cut(s) 607
BsaHI GRCGYC 1 cut(s) 93
BsaJI CCNNGG 3 cut(s) 209, 316, 804
Bse1I ACTGG 3 cut(s) 248, 398, 442
Bse3DI GCAATG 2 cut(s) 543, 690
BseBI CCWGG 2 cut(s) 663, 806
BseCI ATCGAT 1 cut(s) 708
BseDI CCNNGG 3 cut(s) 209, 316, 804
BseGI GGATG 2 cut(s) 370, 511
BseMI GCAATG 2 cut(s) 543, 690
BseMII CTCAG 3 cut(s) 313, 381, 714
BseNI ACTGG 3 cut(s) 248, 398, 442
BseRI GAGGAG 3 cut(s) 7, 35, 643
BseXI GCAGC 1 cut(s) 794
Bsh1236I CGCG 2 cut(s) 211, 427
BshFI GGCC 2 cut(s) 5, 844
BshVI ATCGAT 1 cut(s) 708
BsiHKAI GWGCWC 1 cut(s) 325
BslFI GGGAC 2 cut(s) 408, 787
BsmAI GTCTC 1 cut(s) 641
BsmFI GGGAC 2 cut(s) 408, 787
BsnI GGCC 2 cut(s) 5, 844
Bsp1286I GDGCHC 1 cut(s) 325
Bsp143I GATC 5 cut(s) 220, 262, 310, 454, 655
BspACI CCGC 5 cut(s) 49, 83, 209, 211, 427
BspANI GGCC 2 cut(s) 5, 844
BspCNI CTCAG 3 cut(s) 312, 382, 715
BspDI ATCGAT 1 cut(s) 708
BspFNI CGCG 2 cut(s) 211, 427
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 396
BspPI GGATC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 51
BsrDI GCAATG 2 cut(s) 543, 690
BsrI ACTGG 3 cut(s) 248, 398, 442
BssECI CCNNGG 3 cut(s) 209, 316, 804
BssMI GATC 5 cut(s) 220, 262, 310, 454, 655
BssNI GRCGYC 1 cut(s) 93
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 2 cut(s) 663, 806
Bst4CI ACNGT 2 cut(s) 382, 821
BstACI GRCGYC 1 cut(s) 93
BstBAI YACGTR 1 cut(s) 607
BstC8I GCNNGC 3 cut(s) 136, 635, 842
BstDEI CTNAG 4 cut(s) 299, 390, 624, 723
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 2 cut(s) 370, 511
BstFNI CGCG 2 cut(s) 211, 427
BstHHI GCGC 1 cut(s) 444
BstKTI GATC 5 cut(s) 223, 265, 313, 457, 658
BstMAI GTCTC 1 cut(s) 641
BstMBI GATC 5 cut(s) 220, 262, 310, 454, 655
BstMWI GCNNNNNNNGC 1 cut(s) 131
BstNI CCWGG 2 cut(s) 663, 806
BstSCI CCNGG 2 cut(s) 661, 804
BstSFI CTRYAG 1 cut(s) 772
BstUI CGCG 2 cut(s) 211, 427
BstV1I GCAGC 1 cut(s) 794
BstX2I RGATCY 1 cut(s) 454
BstYI RGATCY 1 cut(s) 454
Bsu15I ATCGAT 1 cut(s) 708
BsuRI GGCC 2 cut(s) 5, 844
BsuTUI ATCGAT 1 cut(s) 708
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 2 cut(s) 370, 511
BtsIMutI CAGTG 2 cut(s) 387, 435
Cac8I GCNNGC 3 cut(s) 136, 635, 842
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 2 cut(s) 395, 620
Cfr42I CCGCGG 1 cut(s) 212
ClaI ATCGAT 1 cut(s) 708
CseI GACGC 1 cut(s) 101
CviAII CATG 5 cut(s) 87, 131, 256, 281, 764
DdeI CTNAG 4 cut(s) 299, 390, 624, 723
DpnI GATC 5 cut(s) 222, 264, 312, 456, 657
DpnII GATC 5 cut(s) 220, 262, 310, 454, 655
EciI GGCGGA 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 323
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 325
Eco32I GATATC 1 cut(s) 589
Eco47I GGWCC 2 cut(s) 395, 620
Eco53kI GAGCTC 1 cut(s) 323
Eco72I CACGTG 1 cut(s) 607
EcoICRI GAGCTC 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 474
EcoRII CCWGG 2 cut(s) 661, 804
EcoRV GATATC 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 316
EcoT22I ATGCAT 1 cut(s) 346
EcoT38I GRGCYC 1 cut(s) 325
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 5 cut(s) 90, 134, 259, 284, 767
FalI AAGNNNNNCTT 2 cut(s) 617, 649
FaqI GGGAC 2 cut(s) 408, 787
FatI CATG 5 cut(s) 86, 130, 255, 280, 763
FbaI TGATCA 2 cut(s) 220, 262
Fnu4HI GCNGC 2 cut(s) 209, 783
FokI GGATG 2 cut(s) 377, 498
FriOI GRGCYC 1 cut(s) 325
Fsp4HI GCNGC 2 cut(s) 209, 783
FspBI CTAG 1 cut(s) 522
GlaI GCGC 1 cut(s) 443
GluI GCNGC 2 cut(s) 209, 783
GsuI CTGGAG 2 cut(s) 130, 549
HaeIII GGCC 2 cut(s) 5, 844
HgaI GACGC 1 cut(s) 101
HhaI GCGC 1 cut(s) 444
Hin1I GRCGYC 1 cut(s) 93
Hin1II CATG 5 cut(s) 90, 134, 259, 284, 767
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HindIII AAGCTT 2 cut(s) 546, 575
HinfI GANTC 5 cut(s) 65, 269, 275, 518, 716
HphI GGTGA 2 cut(s) 320, 554
Hpy166II GTNNAC 1 cut(s) 817
Hpy188I TCNGA 4 cut(s) 64, 70, 220, 274
Hpy188III TCNNGA 7 cut(s) 147, 224, 256, 266, 458, 515, 566
Hpy8I GTNNAC 1 cut(s) 817
Hpy99I CGWCG 2 cut(s) 95, 208
HpyAV CCTTC 3 cut(s) 16, 116, 731
HpyCH4III ACNGT 2 cut(s) 382, 821
HpyCH4IV ACGT 2 cut(s) 159, 606
HpyCH4V TGCA 6 cut(s) 170, 191, 284, 295, 344, 536
HpyF10VI GCNNNNNNNGC 1 cut(s) 131
HpyF3I CTNAG 4 cut(s) 299, 390, 624, 723
HpySE526I ACGT 2 cut(s) 159, 606
Hsp92I GRCGYC 1 cut(s) 93
Hsp92II CATG 5 cut(s) 90, 134, 259, 284, 767
HspAI GCGC 1 cut(s) 442
Ksp22I TGATCA 2 cut(s) 220, 262
KspI CCGCGG 1 cut(s) 212
Kzo9I GATC 5 cut(s) 220, 262, 310, 454, 655
LmnI GCTCC 4 cut(s) 108, 320, 568, 630
Lsp1109I GCAGC 1 cut(s) 794
LweI GCATC 2 cut(s) 179, 217
MaeI CTAG 1 cut(s) 522
MaeII ACGT 2 cut(s) 159, 606
MaeIII GTNAC 2 cut(s) 827, 863
MalI GATC 5 cut(s) 222, 264, 312, 456, 657
MbiI CCGCTC 1 cut(s) 51
MboI GATC 5 cut(s) 220, 262, 310, 454, 655
MboII GAAGA 1 cut(s) 464
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 1 cut(s) 325
MluCI AATT 5 cut(s) 71, 151, 474, 678, 758
MnlI CCTC 8 cut(s) 28, 56, 90, 107, 126, 385, 621, 770
Mph1103I ATGCAT 1 cut(s) 346
MseI TTAA 4 cut(s) 347, 402, 702, 788
MspA1I CMGCKG 2 cut(s) 211, 722
MspR9I CCNGG 2 cut(s) 663, 806
MvaI CCWGG 2 cut(s) 663, 806
MvnI CGCG 2 cut(s) 211, 427
MwoI GCNNNNNNNGC 1 cut(s) 131
NdeII GATC 5 cut(s) 220, 262, 310, 454, 655
NlaIII CATG 5 cut(s) 90, 134, 259, 284, 767
NlaIV GGNNCC 1 cut(s) 396
NsiI ATGCAT 1 cut(s) 346
PagI TCATGA 1 cut(s) 255
PfeI GAWTC 5 cut(s) 65, 269, 275, 518, 716
PkrI GCNGC 2 cut(s) 210, 784
PmaCI CACGTG 1 cut(s) 607
PmlI CACGTG 1 cut(s) 607
Ppu21I YACGTR 1 cut(s) 607
PshBI ATTAAT 1 cut(s) 702
Psp124BI GAGCTC 1 cut(s) 325
Psp1406I AACGTT 1 cut(s) 159
Psp6I CCWGG 2 cut(s) 661, 804
PspCI CACGTG 1 cut(s) 607
PspGI CCWGG 2 cut(s) 661, 804
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 2 cut(s) 395, 620
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 722
SacI GAGCTC 1 cut(s) 325
SacII CCGCGG 1 cut(s) 212
SaqAI TTAA 4 cut(s) 347, 402, 702, 788
SatI GCNGC 2 cut(s) 209, 783
Sau3AI GATC 5 cut(s) 220, 262, 310, 454, 655
Sau96I GGNCC 2 cut(s) 395, 620
ScrFI CCNGG 2 cut(s) 663, 806
SduI GDGCHC 1 cut(s) 325
SfaNI GCATC 2 cut(s) 179, 217
SfcI CTRYAG 1 cut(s) 772
Sfr303I CCGCGG 1 cut(s) 212
SgrBI CCGCGG 1 cut(s) 212
SinI GGWCC 2 cut(s) 395, 620
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 5 cut(s) 71, 151, 474, 678, 758
SsiI CCGC 5 cut(s) 49, 83, 209, 211, 427
SspMI CTAG 1 cut(s) 522
SstI GAGCTC 1 cut(s) 325
StyD4I CCNGG 2 cut(s) 661, 804
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 382, 821
TaiI ACGT 2 cut(s) 162, 609
TaqI TCGA 3 cut(s) 478, 658, 708
TasI AATT 5 cut(s) 71, 151, 474, 678, 758
TauI GCSGC 1 cut(s) 211
TfiI GAWTC 5 cut(s) 65, 269, 275, 518, 716
Tru1I TTAA 4 cut(s) 347, 402, 702, 788
Tru9I TTAA 4 cut(s) 347, 402, 702, 788
TscAI CASTG 2 cut(s) 394, 442
TseI GCWGC 1 cut(s) 782
TspDTI ATGAA 4 cut(s) 119, 189, 407, 752
TspRI CASTG 2 cut(s) 394, 442
VpaK11BI GGWCC 2 cut(s) 395, 620
VspI ATTAAT 1 cut(s) 702
XapI RAATTY 4 cut(s) 71, 151, 474, 758
XspI CTAG 1 cut(s) 522
Zsp2I ATGCAT 1 cut(s) 346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.