pycom07g04910

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0014353
Physical Location & Seq
Forward (+)
4226433 .. 4233816
7384 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g04910.10

Sequence Viewer

Length: 876 bp
ATGGCGCTCTTTGCTTCTTCCATCAATCTCCATCTATCCAAAATCTCCCATTCCGAATTTGCCCCTCAGCCCCGCCCCTCCCCGTACGACCCCCCCAAACTCCTCCCGCTTCCTGCCGTATGCTCCTCCCAACGTGCTGCCGCCAACGCTTTCTCCAGAGAAAGGAACATTGTGCGTGCGTCGATGAATGGTCTGAATGCAGTTGGATCAAGCTCGTTGAAGTCTGATCAAGGTGCTGATTATCTTCCGATGCCAGTTGTTATGATCGATCAAGATTCAGATTCTGATGCAACTATTGTGCAACTCAGCTTCGGTGATCGCCTTGGAGCACTCATTGATACGATGAATGCATTGAAAGATTTGGGATTGGATGTGGCTAAGGGAACTGTCACCACTGAGGGACCAGTCAAACAAACGAAGTTTTATATAACACGATTAGACACCGGGCGCAAAGTTGAAGATCCTGATATGTTAGAGAGAATTCGACTTACCATCATCAACAATCTTTTGAAGTATCATCCAGAATCTAGTCAGCATCTAGCAATGGGTGAAGCATTTGGAATAAAAGCTCCAGAGAAGAAGCTTGATGTGGATATTGCAACTCATATACATGTGAAGGAAGATGGACCTAAGAAGAGCTTGCTATACATAGAGACAGCAGATCGACCTGGGTTGCTAGTAGAAATCATAAAAATCATTGCTGATGTTAATATTGACGTGGAATCAGCCGAGATTGATACAGAAGGGTTGGTAGCCAAAGATAAATTTCATGTTAGCTACAGAGGGGCAGCATTGAACAGTTCCTTGTCTCAGGTGTTGGTGAACTGTTTGCGTTACTACATCCGGAGGCCAGAAACAGATATAGATAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

292

Amino Acids

31.97

Weight (kDa)

5.68

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 843
AciI CCGC 3 cut(s) 73, 107, 141
AclWI GGATC 2 cut(s) 214, 455
AcsI RAATTY 3 cut(s) 56, 480, 764
AfaI GTAC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 162
AflIII ACRYGT 1 cut(s) 610
AgsI TTSAA 5 cut(s) 220, 355, 458, 511, 796
AjiI CACGTC 1 cut(s) 718
AjnI CCWGG 1 cut(s) 667
AluBI AGCT 6 cut(s) 213, 309, 569, 583, 639, 777
AluI AGCT 6 cut(s) 213, 309, 569, 583, 639, 777
Alw21I GWGCWC 1 cut(s) 331
Alw26I GTCTC 2 cut(s) 647, 813
AlwI GGATC 2 cut(s) 214, 455
AlwNI CAGNNNCTG 1 cut(s) 284
Aor13HI TCCGGA 1 cut(s) 843
AoxI GGCC 1 cut(s) 848
ApeKI GCWGC 2 cut(s) 137, 788
ApoI RAATTY 3 cut(s) 56, 480, 764
AspLEI GCGC 2 cut(s) 7, 450
AspS9I GGNCC 2 cut(s) 401, 626
AsuC2I CCSGG 1 cut(s) 445
AsuHPI GGTGA 4 cut(s) 326, 382, 560, 832
AvaII GGWCC 2 cut(s) 401, 626
Bbv12I GWGCWC 1 cut(s) 331
BbvCI CCTCAGC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 124, 800
BccI CCATC 4 cut(s) 29, 39, 500, 617
BceAI ACGGC 1 cut(s) 101
BciT130I CCWGG 1 cut(s) 669
BclI TGATCA 1 cut(s) 226
BcnI CCSGG 1 cut(s) 445
BcoDI GTCTC 2 cut(s) 647, 813
BfaI CTAG 3 cut(s) 528, 539, 677
BfmI CTRYAG 1 cut(s) 778
BfoI RGCGCY 1 cut(s) 8
BisI GCNGC 3 cut(s) 138, 141, 789
BlsI GCNGC 3 cut(s) 139, 142, 790
Bme1390I CCNGG 2 cut(s) 445, 669
Bme18I GGWCC 2 cut(s) 401, 626
BmgBI CACGTC 1 cut(s) 718
BmgT120I GGNCC 2 cut(s) 401, 626
BmiI GGNNCC 1 cut(s) 402
BmrFI CCNGG 2 cut(s) 445, 669
BmsI GCATC 3 cut(s) 240, 277, 544
BpmI CTGGAG 2 cut(s) 139, 555
Bpu10I CCTNAGC 2 cut(s) 66, 378
BpuMI CCSGG 1 cut(s) 445
Bsa29I ATCGAT 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 322, 668
BsaWI WCCGGW 1 cut(s) 843
BsaXI ACNNNNNCTCC 2 cut(s) 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse1I ACTGG 2 cut(s) 254, 404
Bse3DI GCAATG 2 cut(s) 549, 696
BseAI TCCGGA 1 cut(s) 843
BseBI CCWGG 1 cut(s) 669
BseCI ATCGAT 1 cut(s) 267
BseDI CCNNGG 2 cut(s) 322, 668
BseGI GGATG 3 cut(s) 376, 517, 840
BseLI CCNNNNNNNGG 1 cut(s) 162
BseMI GCAATG 2 cut(s) 549, 696
BseMII CTCAG 4 cut(s) 80, 319, 387, 824
BseNI ACTGG 2 cut(s) 254, 404
BseRI GAGGAG 2 cut(s) 92, 115
BseXI GCAGC 2 cut(s) 124, 800
BshFI GGCC 1 cut(s) 850
BshVI ATCGAT 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 331
BsiSI CCGG 2 cut(s) 444, 844
BsiWI CGTACG 1 cut(s) 84
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 1 cut(s) 162
BsmAI GTCTC 2 cut(s) 647, 813
BsmFI GGGAC 1 cut(s) 414
BsmI GAATGC 2 cut(s) 202, 352
BsnI GGCC 1 cut(s) 850
Bsp1286I GDGCHC 1 cut(s) 331
Bsp13I TCCGGA 1 cut(s) 843
Bsp143I GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
BspACI CCGC 3 cut(s) 73, 107, 141
BspANI GGCC 1 cut(s) 850
BspCNI CTCAG 4 cut(s) 79, 318, 388, 823
BspDI ATCGAT 1 cut(s) 267
BspEI TCCGGA 1 cut(s) 843
BspLI GGNNCC 1 cut(s) 402
BspPI GGATC 2 cut(s) 214, 455
BspQI GCTCTTC 1 cut(s) 629
BsrDI GCAATG 2 cut(s) 549, 696
BsrI ACTGG 2 cut(s) 254, 404
BssECI CCNNGG 2 cut(s) 322, 668
BssMI GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
BssT1I CCWWGG 1 cut(s) 322
Bst2UI CCWGG 1 cut(s) 669
Bst4CI ACNGT 3 cut(s) 388, 800, 827
Bst6I CTCTTC 1 cut(s) 629
BstC8I GCNNGC 2 cut(s) 177, 641
BstDEI CTNAG 6 cut(s) 66, 305, 378, 396, 630, 810
BstF5I GGATG 3 cut(s) 376, 517, 840
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 2 cut(s) 7, 450
BstKTI GATC 7 cut(s) 209, 229, 267, 271, 319, 463, 664
BstMAI GTCTC 2 cut(s) 647, 813
BstMBI GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
BstMWI GCNNNNNNNGC 2 cut(s) 11, 146
BstNI CCWGG 1 cut(s) 669
BstNSI RCATGY 1 cut(s) 614
BstSCI CCNGG 2 cut(s) 443, 667
BstSFI CTRYAG 1 cut(s) 778
BstV1I GCAGC 2 cut(s) 124, 800
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
Bsu15I ATCGAT 1 cut(s) 267
BsuRI GGCC 1 cut(s) 850
BsuTUI ATCGAT 1 cut(s) 267
BtrI CACGTC 1 cut(s) 718
BtsCI GGATG 3 cut(s) 376, 517, 840
BtsIMutI CAGTG 1 cut(s) 393
Cac8I GCNNGC 2 cut(s) 177, 641
CaiI CAGNNNCTG 1 cut(s) 284
CfoI GCGC 2 cut(s) 7, 450
Cfr13I GGNCC 2 cut(s) 401, 626
ClaI ATCGAT 1 cut(s) 267
CseI GACGC 1 cut(s) 168
Csp6I GTAC 1 cut(s) 85
CviAII CATG 2 cut(s) 611, 770
CviQI GTAC 1 cut(s) 85
DdeI CTNAG 6 cut(s) 66, 305, 378, 396, 630, 810
DpnI GATC 7 cut(s) 208, 228, 266, 270, 318, 462, 663
DpnII GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
Eam1104I CTCTTC 1 cut(s) 629
EarI CTCTTC 1 cut(s) 629
Eco130I CCWWGG 1 cut(s) 322
Eco47I GGWCC 2 cut(s) 401, 626
EcoRI GAATTC 1 cut(s) 480
EcoRII CCWGG 1 cut(s) 667
EcoT14I CCWWGG 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 322
FaeI CATG 2 cut(s) 614, 773
FalI AAGNNNNNCTT 2 cut(s) 623, 655
FaqI GGGAC 1 cut(s) 414
FatI CATG 2 cut(s) 610, 769
FauI CCCGC 2 cut(s) 80, 114
FbaI TGATCA 1 cut(s) 226
Fnu4HI GCNGC 3 cut(s) 138, 141, 789
FokI GGATG 3 cut(s) 383, 504, 827
Fsp4HI GCNGC 3 cut(s) 138, 141, 789
FspBI CTAG 3 cut(s) 528, 539, 677
GlaI GCGC 2 cut(s) 6, 449
GluI GCNGC 3 cut(s) 138, 141, 789
GsuI CTGGAG 2 cut(s) 139, 555
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 850
HapII CCGG 2 cut(s) 444, 844
HgaI GACGC 1 cut(s) 168
HhaI GCGC 2 cut(s) 7, 450
Hin1II CATG 2 cut(s) 614, 773
Hin6I GCGC 2 cut(s) 5, 448
HinP1I GCGC 2 cut(s) 5, 448
HindIII AAGCTT 1 cut(s) 581
HinfI GANTC 4 cut(s) 275, 281, 524, 722
HpaII CCGG 2 cut(s) 444, 844
HphI GGTGA 4 cut(s) 326, 382, 560, 832
Hpy166II GTNNAC 1 cut(s) 823
Hpy188I TCNGA 6 cut(s) 55, 195, 226, 249, 280, 286
Hpy188III TCNNGA 6 cut(s) 156, 272, 464, 521, 572, 844
Hpy8I GTNNAC 1 cut(s) 823
Hpy99I CGWCG 1 cut(s) 184
HpyAV CCTTC 2 cut(s) 610, 737
HpyCH4III ACNGT 3 cut(s) 388, 800, 827
HpyCH4IV ACGT 2 cut(s) 133, 717
HpyCH4V TGCA 5 cut(s) 200, 290, 301, 350, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 11, 146
HpyF3I CTNAG 6 cut(s) 66, 305, 378, 396, 630, 810
HpySE526I ACGT 2 cut(s) 133, 717
Hsp92II CATG 2 cut(s) 614, 773
HspAI GCGC 2 cut(s) 5, 448
Kpn2I TCCGGA 1 cut(s) 843
Ksp22I TGATCA 1 cut(s) 226
Kzo9I GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
LguI GCTCTTC 1 cut(s) 629
LmnI GCTCC 3 cut(s) 128, 326, 574
Lsp1109I GCAGC 2 cut(s) 124, 800
LweI GCATC 3 cut(s) 240, 277, 544
MaeI CTAG 3 cut(s) 528, 539, 677
MaeII ACGT 2 cut(s) 133, 717
MaeIII GTNAC 3 cut(s) 388, 833, 869
MalI GATC 7 cut(s) 208, 228, 266, 270, 318, 462, 663
MboI GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
MboII GAAGA 6 cut(s) 9, 236, 470, 589, 632, 646
MflI RGATCY 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 331
MluCI AATT 3 cut(s) 56, 480, 764
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 7 cut(s) 75, 88, 113, 136, 391, 776, 840
Mph1103I ATGCAT 1 cut(s) 352
MroI TCCGGA 1 cut(s) 843
MseI TTAA 1 cut(s) 708
MslI CAYNNNNRTG 1 cut(s) 609
MspI CCGG 2 cut(s) 444, 844
MspR9I CCNGG 2 cut(s) 445, 669
Mva1269I GAATGC 2 cut(s) 202, 352
MvaI CCWGG 1 cut(s) 669
MwoI GCNNNNNNNGC 2 cut(s) 11, 146
NciI CCSGG 1 cut(s) 445
NdeII GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
NlaIII CATG 2 cut(s) 614, 773
NlaIV GGNNCC 1 cut(s) 402
NmeAIII GCCGAG 1 cut(s) 754
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 352
NspI RCATGY 1 cut(s) 614
PciI ACATGT 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 629
PctI GAATGC 2 cut(s) 202, 352
PfeI GAWTC 4 cut(s) 275, 281, 524, 722
Pfl23II CGTACG 1 cut(s) 84
PkrI GCNGC 3 cut(s) 139, 142, 790
PscI ACATGT 1 cut(s) 610
Psp6I CCWGG 1 cut(s) 667
PspGI CCWGG 1 cut(s) 667
PspLI CGTACG 1 cut(s) 84
PspN4I GGNNCC 1 cut(s) 402
PspPI GGNCC 2 cut(s) 401, 626
PstNI CAGNNNCTG 1 cut(s) 284
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 1 cut(s) 86
RsaNI GTAC 1 cut(s) 85
RseI CAYNNNNRTG 1 cut(s) 609
SapI GCTCTTC 1 cut(s) 629
SaqAI TTAA 1 cut(s) 708
SatI GCNGC 3 cut(s) 138, 141, 789
Sau3AI GATC 7 cut(s) 206, 226, 264, 268, 316, 460, 661
Sau96I GGNCC 2 cut(s) 401, 626
ScrFI CCNGG 2 cut(s) 445, 669
SduI GDGCHC 1 cut(s) 331
SfaNI GCATC 3 cut(s) 240, 277, 544
SfcI CTRYAG 1 cut(s) 778
SinI GGWCC 2 cut(s) 401, 626
SmiMI CAYNNNNRTG 1 cut(s) 609
Sse9I AATT 3 cut(s) 56, 480, 764
SsiI CCGC 3 cut(s) 73, 107, 141
SspI AATATT 1 cut(s) 712
SspMI CTAG 3 cut(s) 528, 539, 677
StyD4I CCNGG 2 cut(s) 443, 667
StyI CCWWGG 1 cut(s) 322
TaaI ACNGT 3 cut(s) 388, 800, 827
TaiI ACGT 2 cut(s) 136, 720
TaqI TCGA 4 cut(s) 182, 267, 484, 664
TasI AATT 3 cut(s) 56, 480, 764
TauI GCSGC 1 cut(s) 143
TfiI GAWTC 4 cut(s) 275, 281, 524, 722
Tru1I TTAA 1 cut(s) 708
Tru9I TTAA 1 cut(s) 708
TscAI CASTG 1 cut(s) 400
TseFI GTSAC 1 cut(s) 388
TseI GCWGC 2 cut(s) 137, 788
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 3 cut(s) 200, 359, 758
TspRI CASTG 1 cut(s) 400
VpaK11BI GGWCC 2 cut(s) 401, 626
XapI RAATTY 3 cut(s) 56, 480, 764
XceI RCATGY 1 cut(s) 614
XspI CTAG 3 cut(s) 528, 539, 677
Zsp2I ATGCAT 1 cut(s) 352
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.