Rmu_ssc0000161.1_g000018

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000161.1
OG0014353
Physical Location & Seq
Reverse (-)
72553 .. 76451
3899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000161.1_g000018.1.cds

Sequence Viewer

Length: 627 bp
atgccagttgtcatgattgatcaagattcagattctgatgcaactattgtgcaactcagctttggtgatcgcctcggagctcttattgacacgataaatgcattaaaagatttgggattggatgtagcaaagggaactgttaccactgagggaccagttaaacagacgaagtttcatataacgcggttagacactgggcgcaaagttgaagatcctgatatgttagagagaattcgacttaccatcatcaacaatcttttgaagtatcatccggaatctagtcagctacttgcaatgggtgaagcttttggaataaaagctccggaaaagaagcttgatgttgatatcgcaactcatatacacgtgaagcaagatggacctaagaggagcttgctttatatagagacagcagatcgaccaggattgcttgtagaaattatcaaaatcattgctgacattaatatcgatgtggaatcagctgagattgatacagaaggtttggttgctaaagataaatttcatgtcagctacagaggggcagccttaaatagttccttgtcccaggtgttagtgaactgtctgcgttactacattcgcaggccagaaacagatatagatagttactaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

208

Amino Acids

23.11

Weight (kDa)

5.14

Isoelectric Point (pI)

40.62

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 184
AccIII TCCGGA 2 cut(s) 271, 322
AciI CCGC 1 cut(s) 184
AclWI GGATC 1 cut(s) 206
AcsI RAATTY 2 cut(s) 231, 515
AcvI CACGTG 1 cut(s) 364
AflIII ACRYGT 1 cut(s) 361
AgsI TTSAA 2 cut(s) 209, 262
AjnI CCWGG 2 cut(s) 418, 561
AluBI AGCT 9 cut(s) 60, 80, 286, 305, 320, 334, 390, 479, 528
AluI AGCT 9 cut(s) 60, 80, 286, 305, 320, 334, 390, 479, 528
Alw21I GWGCWC 1 cut(s) 82
Alw26I GTCTC 1 cut(s) 398
AlwI GGATC 1 cut(s) 206
AlwNI CAGNNNCTG 1 cut(s) 35
Aor13HI TCCGGA 2 cut(s) 271, 322
AoxI GGCC 1 cut(s) 599
ApeKI GCWGC 1 cut(s) 539
ApoI RAATTY 2 cut(s) 231, 515
AseI ATTAAT 1 cut(s) 459
AspLEI GCGC 1 cut(s) 201
AspS9I GGNCC 2 cut(s) 152, 377
AsuHPI GGTGA 2 cut(s) 77, 311
AvaII GGWCC 2 cut(s) 152, 377
BanII GRGCYC 1 cut(s) 82
BbrPI CACGTG 1 cut(s) 364
Bbv12I GWGCWC 1 cut(s) 82
BbvI GCAGC 1 cut(s) 551
BccI CCATC 2 cut(s) 251, 368
BciT130I CCWGG 2 cut(s) 420, 563
BclI TGATCA 1 cut(s) 19
BcoDI GTCTC 1 cut(s) 398
BfaI CTAG 1 cut(s) 279
BfmI CTRYAG 1 cut(s) 529
BisI GCNGC 1 cut(s) 540
BlsI GCNGC 1 cut(s) 541
Bme1390I CCNGG 2 cut(s) 420, 563
Bme18I GGWCC 2 cut(s) 152, 377
BmgT120I GGNCC 2 cut(s) 152, 377
BmiI GGNNCC 1 cut(s) 153
BmrFI CCNGG 2 cut(s) 420, 563
BmrI ACTGGG 1 cut(s) 204
BmsI GCATC 1 cut(s) 28
BmuI ACTGGG 1 cut(s) 204
Bsa29I ATCGAT 1 cut(s) 465
BsaAI YACGTR 1 cut(s) 364
BsaJI CCNNGG 2 cut(s) 73, 561
BsaWI WCCGGW 2 cut(s) 271, 322
Bse1I ACTGG 3 cut(s) 5, 155, 199
Bse3DI GCAATG 2 cut(s) 300, 447
BseAI TCCGGA 2 cut(s) 271, 322
BseBI CCWGG 2 cut(s) 420, 563
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 73, 561
BseGI GGATG 2 cut(s) 127, 268
BseMI GCAATG 2 cut(s) 300, 447
BseMII CTCAG 3 cut(s) 70, 138, 471
BseNI ACTGG 3 cut(s) 5, 155, 199
BseRI GAGGAG 1 cut(s) 400
BseXI GCAGC 1 cut(s) 551
Bsh1236I CGCG 1 cut(s) 184
BshFI GGCC 1 cut(s) 601
BshVI ATCGAT 1 cut(s) 465
BsiHKAI GWGCWC 1 cut(s) 82
BsiSI CCGG 2 cut(s) 272, 323
BslFI GGGAC 2 cut(s) 165, 544
BsmAI GTCTC 1 cut(s) 398
BsmFI GGGAC 2 cut(s) 165, 544
BsnI GGCC 1 cut(s) 601
Bsp1286I GDGCHC 1 cut(s) 82
Bsp13I TCCGGA 2 cut(s) 271, 322
Bsp143I GATC 4 cut(s) 19, 67, 211, 412
BspACI CCGC 1 cut(s) 184
BspANI GGCC 1 cut(s) 601
BspCNI CTCAG 3 cut(s) 69, 139, 472
BspDI ATCGAT 1 cut(s) 465
BspEI TCCGGA 2 cut(s) 271, 322
BspFNI CGCG 1 cut(s) 184
BspHI TCATGA 1 cut(s) 12
BspLI GGNNCC 1 cut(s) 153
BspPI GGATC 1 cut(s) 206
BsrDI GCAATG 2 cut(s) 300, 447
BsrI ACTGG 3 cut(s) 5, 155, 199
BssECI CCNNGG 2 cut(s) 73, 561
BssMI GATC 4 cut(s) 19, 67, 211, 412
Bst2UI CCWGG 2 cut(s) 420, 563
Bst4CI ACNGT 2 cut(s) 139, 578
BstBAI YACGTR 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 392, 599
BstDEI CTNAG 4 cut(s) 56, 147, 381, 480
BstF5I GGATG 2 cut(s) 127, 268
BstFNI CGCG 1 cut(s) 184
BstHHI GCGC 1 cut(s) 201
BstKTI GATC 4 cut(s) 22, 70, 214, 415
BstMAI GTCTC 1 cut(s) 398
BstMBI GATC 4 cut(s) 19, 67, 211, 412
BstNI CCWGG 2 cut(s) 420, 563
BstSCI CCNGG 2 cut(s) 418, 561
BstSFI CTRYAG 1 cut(s) 529
BstUI CGCG 1 cut(s) 184
BstV1I GCAGC 1 cut(s) 551
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
Bsu15I ATCGAT 1 cut(s) 465
BsuRI GGCC 1 cut(s) 601
BsuTUI ATCGAT 1 cut(s) 465
BtsCI GGATG 2 cut(s) 127, 268
BtsIMutI CAGTG 2 cut(s) 144, 192
Cac8I GCNNGC 2 cut(s) 392, 599
CaiI CAGNNNCTG 1 cut(s) 35
CciI TCATGA 1 cut(s) 12
CfoI GCGC 1 cut(s) 201
Cfr13I GGNCC 2 cut(s) 152, 377
ClaI ATCGAT 1 cut(s) 465
CviAII CATG 2 cut(s) 13, 521
DdeI CTNAG 4 cut(s) 56, 147, 381, 480
DpnI GATC 4 cut(s) 21, 69, 213, 414
DpnII GATC 4 cut(s) 19, 67, 211, 412
Ecl136II GAGCTC 1 cut(s) 80
Eco24I GRGCYC 1 cut(s) 82
Eco32I GATATC 1 cut(s) 346
Eco47I GGWCC 2 cut(s) 152, 377
Eco53kI GAGCTC 1 cut(s) 80
Eco72I CACGTG 1 cut(s) 364
EcoICRI GAGCTC 1 cut(s) 80
EcoRI GAATTC 1 cut(s) 231
EcoRII CCWGG 2 cut(s) 418, 561
EcoRV GATATC 1 cut(s) 346
EcoT22I ATGCAT 1 cut(s) 103
EcoT38I GRGCYC 1 cut(s) 82
FaeI CATG 2 cut(s) 16, 524
FalI AAGNNNNNCTT 2 cut(s) 374, 406
FaqI GGGAC 2 cut(s) 165, 544
FatI CATG 2 cut(s) 12, 520
FbaI TGATCA 1 cut(s) 19
Fnu4HI GCNGC 1 cut(s) 540
FokI GGATG 2 cut(s) 134, 255
FriOI GRGCYC 1 cut(s) 82
Fsp4HI GCNGC 1 cut(s) 540
FspBI CTAG 1 cut(s) 279
GlaI GCGC 1 cut(s) 200
GluI GCNGC 1 cut(s) 540
HaeIII GGCC 1 cut(s) 601
HapII CCGG 2 cut(s) 272, 323
HhaI GCGC 1 cut(s) 201
Hin1II CATG 2 cut(s) 16, 524
Hin6I GCGC 1 cut(s) 199
HinP1I GCGC 1 cut(s) 199
HindIII AAGCTT 2 cut(s) 303, 332
HinfI GANTC 4 cut(s) 26, 32, 275, 473
HpaII CCGG 2 cut(s) 272, 323
HphI GGTGA 2 cut(s) 77, 311
Hpy166II GTNNAC 1 cut(s) 574
Hpy188I TCNGA 3 cut(s) 31, 37, 77
Hpy188III TCNNGA 5 cut(s) 13, 23, 215, 272, 323
Hpy8I GTNNAC 1 cut(s) 574
HpyAV CCTTC 1 cut(s) 488
HpyCH4III ACNGT 2 cut(s) 139, 578
HpyCH4IV ACGT 1 cut(s) 363
HpyCH4V TGCA 4 cut(s) 41, 52, 101, 293
HpyF3I CTNAG 4 cut(s) 56, 147, 381, 480
HpySE526I ACGT 1 cut(s) 363
Hsp92II CATG 2 cut(s) 16, 524
HspAI GCGC 1 cut(s) 199
Kpn2I TCCGGA 2 cut(s) 271, 322
Ksp22I TGATCA 1 cut(s) 19
Kzo9I GATC 4 cut(s) 19, 67, 211, 412
LmnI GCTCC 3 cut(s) 77, 325, 387
Lsp1109I GCAGC 1 cut(s) 551
LweI GCATC 1 cut(s) 28
MaeI CTAG 1 cut(s) 279
MaeII ACGT 1 cut(s) 363
MaeIII GTNAC 3 cut(s) 139, 584, 620
MalI GATC 4 cut(s) 21, 69, 213, 414
MboI GATC 4 cut(s) 19, 67, 211, 412
MboII GAAGA 1 cut(s) 221
MflI RGATCY 1 cut(s) 211
MhlI GDGCHC 1 cut(s) 82
MluCI AATT 3 cut(s) 231, 435, 515
MnlI CCTC 4 cut(s) 83, 142, 378, 527
Mph1103I ATGCAT 1 cut(s) 103
MroI TCCGGA 2 cut(s) 271, 322
MseI TTAA 4 cut(s) 104, 159, 459, 545
MspA1I CMGCKG 1 cut(s) 479
MspI CCGG 2 cut(s) 272, 323
MspR9I CCNGG 2 cut(s) 420, 563
MvaI CCWGG 2 cut(s) 420, 563
MvnI CGCG 1 cut(s) 184
NdeII GATC 4 cut(s) 19, 67, 211, 412
NlaIII CATG 2 cut(s) 16, 524
NlaIV GGNNCC 1 cut(s) 153
NsiI ATGCAT 1 cut(s) 103
PagI TCATGA 1 cut(s) 12
PfeI GAWTC 4 cut(s) 26, 32, 275, 473
PkrI GCNGC 1 cut(s) 541
PmaCI CACGTG 1 cut(s) 364
PmlI CACGTG 1 cut(s) 364
Ppu21I YACGTR 1 cut(s) 364
PshBI ATTAAT 1 cut(s) 459
Psp124BI GAGCTC 1 cut(s) 82
Psp6I CCWGG 2 cut(s) 418, 561
PspCI CACGTG 1 cut(s) 364
PspGI CCWGG 2 cut(s) 418, 561
PspN4I GGNNCC 1 cut(s) 153
PspPI GGNCC 2 cut(s) 152, 377
PstNI CAGNNNCTG 1 cut(s) 35
PsuI RGATCY 1 cut(s) 211
PvuII CAGCTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 82
SaqAI TTAA 4 cut(s) 104, 159, 459, 545
SatI GCNGC 1 cut(s) 540
Sau3AI GATC 4 cut(s) 19, 67, 211, 412
Sau96I GGNCC 2 cut(s) 152, 377
ScrFI CCNGG 2 cut(s) 420, 563
SduI GDGCHC 1 cut(s) 82
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 1 cut(s) 529
SinI GGWCC 2 cut(s) 152, 377
Sse9I AATT 3 cut(s) 231, 435, 515
SsiI CCGC 1 cut(s) 184
SspMI CTAG 1 cut(s) 279
SstI GAGCTC 1 cut(s) 82
StyD4I CCNGG 2 cut(s) 418, 561
TaaI ACNGT 2 cut(s) 139, 578
TaiI ACGT 1 cut(s) 366
TaqI TCGA 3 cut(s) 235, 415, 465
TasI AATT 3 cut(s) 231, 435, 515
TfiI GAWTC 4 cut(s) 26, 32, 275, 473
Tru1I TTAA 4 cut(s) 104, 159, 459, 545
Tru9I TTAA 4 cut(s) 104, 159, 459, 545
TscAI CASTG 2 cut(s) 151, 199
TseI GCWGC 1 cut(s) 539
TspDTI ATGAA 2 cut(s) 164, 509
TspRI CASTG 2 cut(s) 151, 199
VpaK11BI GGWCC 2 cut(s) 152, 377
VspI ATTAAT 1 cut(s) 459
XapI RAATTY 2 cut(s) 231, 515
XspI CTAG 1 cut(s) 279
Zsp2I ATGCAT 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.