Prupe.2G077000_v2.0.a1

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0014353
Physical Location & Seq
Forward (+)
11880517 .. 11888648
8132 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G077000.1

Sequence Viewer

Length: 882 bp
ATGGCCTTCACCAACTCGGCTCTCTTTGCTTCTTCTCTCACTCTCCATCGATCCAAAATTTCCGATTCCGAATTTGCCCTTCCGCCTCACCTCTCTCCATATGAGCCTTCCAAGCTCCTCCCATTTCCTACCATATACTCTCAACATGCTGACACTTTCTCCAGAAAAATGAACACTGTGCGTGCGTCTAACAATGGTCTGAATGCAGTTGGGTCAACCTCGTTGAAGTCTGATCAAGATGGTGATTATGTTCCGATGCCAGTTGTTATGATCGATCAAGATTCAGATTCTGATGCAACTATTGTGCAACTCAGCTTTGGTGATCGCCTTGGAGCTCTCATTGATACGATCAATGCATTGAAAGATTTGGGATTGGATGTGGTAAAGGGAACTGTTACCACTGAGGGACCAGTCAAACAAACAAAATTTTATATAACGTGGTTAGACACTGGGCGCAAAGTTGAAGATCCTGATATGTTAGAGAGAATTCGACTTACCATCATCAACAATCTTTTGAAGTATCATCCAGAATCTAGTCAGCTACTAGCAATGGGTGAAGCATTTGGAATAAAAGGTCCAGAGAAGAAGCTTGATGTTGATATAGCAACTCATATACATGTGAAGGAAGATGGGCCTAAGAGGAGCTTGCTTTACATTGAGACAGCAGATCGACCTGGGTTGCTAGTAGAAATCATCAAAATCATTGCTGATGTTAATATCGATGTGGAATCAGCTGAGATTGATACAGAAGGCTTGGTAGCCAAAGATACATTTCATGTCAGCTACAGAGGGGCAGCGTTAAACAGTTCCTTGTCTCAGGTGTTAGTGAACTGTCTACGTTACTACATCCGGAGGCCAGAAACGGATATAGATAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

294

Amino Acids

32.4

Weight (kDa)

5.11

Isoelectric Point (pI)

39.97

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 835
AccIII TCCGGA 1 cut(s) 849
AciI CCGC 1 cut(s) 83
AclWI GGATC 2 cut(s) 45, 461
AcsI RAATTY 4 cut(s) 57, 71, 425, 486
AflIII ACRYGT 1 cut(s) 616
AgsI TTSAA 4 cut(s) 226, 361, 464, 517
AjnI CCWGG 1 cut(s) 673
AluBI AGCT 8 cut(s) 115, 315, 335, 541, 589, 645, 734, 783
AluI AGCT 8 cut(s) 115, 315, 335, 541, 589, 645, 734, 783
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 2 cut(s) 653, 819
AlwI GGATC 2 cut(s) 45, 461
AlwNI CAGNNNCTG 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 849
AoxI GGCC 3 cut(s) 3, 632, 854
ApeKI GCWGC 1 cut(s) 794
ApoI RAATTY 4 cut(s) 57, 71, 425, 486
AspLEI GCGC 1 cut(s) 456
AspS9I GGNCC 3 cut(s) 407, 575, 632
AsuHPI GGTGA 4 cut(s) 80, 254, 332, 566
AvaII GGWCC 2 cut(s) 407, 575
BanII GRGCYC 1 cut(s) 337
Bbv12I GWGCWC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 806
BccI CCATC 4 cut(s) 54, 233, 506, 623
BciT130I CCWGG 1 cut(s) 675
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 653, 819
BfaI CTAG 3 cut(s) 534, 545, 683
BfmI CTRYAG 1 cut(s) 784
BisI GCNGC 1 cut(s) 795
BlsI GCNGC 1 cut(s) 796
Bme1390I CCNGG 1 cut(s) 675
Bme18I GGWCC 2 cut(s) 407, 575
BmgT120I GGNCC 3 cut(s) 407, 575, 632
BmiI GGNNCC 1 cut(s) 408
BmrFI CCNGG 1 cut(s) 675
BmrI ACTGGG 1 cut(s) 459
BmsI GCATC 2 cut(s) 246, 283
BmuI ACTGGG 1 cut(s) 459
BpmI CTGGAG 1 cut(s) 145
Bsa29I ATCGAT 3 cut(s) 49, 273, 720
BsaJI CCNNGG 2 cut(s) 328, 674
BsaWI WCCGGW 1 cut(s) 849
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bse1I ACTGG 3 cut(s) 260, 410, 454
Bse3DI GCAATG 2 cut(s) 555, 702
BseAI TCCGGA 1 cut(s) 849
BseBI CCWGG 1 cut(s) 675
BseCI ATCGAT 3 cut(s) 49, 273, 720
BseDI CCNNGG 2 cut(s) 328, 674
BseGI GGATG 3 cut(s) 382, 523, 846
BseMI GCAATG 2 cut(s) 555, 702
BseMII CTCAG 4 cut(s) 325, 393, 726, 830
BseNI ACTGG 3 cut(s) 260, 410, 454
BseRI GAGGAG 2 cut(s) 107, 655
BseXI GCAGC 1 cut(s) 806
BshFI GGCC 3 cut(s) 5, 634, 856
BshVI ATCGAT 3 cut(s) 49, 273, 720
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 1 cut(s) 850
BslFI GGGAC 1 cut(s) 420
BsmAI GTCTC 2 cut(s) 653, 819
BsmFI GGGAC 1 cut(s) 420
BsmI GAATGC 1 cut(s) 208
BsnI GGCC 3 cut(s) 5, 634, 856
Bsp1286I GDGCHC 1 cut(s) 337
Bsp13I TCCGGA 1 cut(s) 849
Bsp143I GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
BspACI CCGC 1 cut(s) 83
BspANI GGCC 3 cut(s) 5, 634, 856
BspCNI CTCAG 4 cut(s) 324, 394, 727, 829
BspDI ATCGAT 3 cut(s) 49, 273, 720
BspEI TCCGGA 1 cut(s) 849
BspLI GGNNCC 1 cut(s) 408
BspPI GGATC 2 cut(s) 45, 461
BsrDI GCAATG 2 cut(s) 555, 702
BsrI ACTGG 3 cut(s) 260, 410, 454
BssECI CCNNGG 2 cut(s) 328, 674
BssMI GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
BssT1I CCWWGG 1 cut(s) 328
Bst2UI CCWGG 1 cut(s) 675
Bst4CI ACNGT 4 cut(s) 178, 394, 806, 833
BstC8I GCNNGC 2 cut(s) 183, 647
BstDEI CTNAG 5 cut(s) 311, 402, 636, 735, 816
BstF5I GGATG 3 cut(s) 382, 523, 846
BstHHI GCGC 1 cut(s) 456
BstKTI GATC 8 cut(s) 53, 235, 273, 277, 325, 351, 469, 670
BstMAI GTCTC 2 cut(s) 653, 819
BstMBI GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
BstMWI GCNNNNNNNGC 2 cut(s) 26, 112
BstNI CCWGG 1 cut(s) 675
BstNSI RCATGY 2 cut(s) 149, 620
BstSCI CCNGG 1 cut(s) 673
BstSFI CTRYAG 1 cut(s) 784
BstV1I GCAGC 1 cut(s) 806
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
Bsu15I ATCGAT 3 cut(s) 49, 273, 720
BsuRI GGCC 3 cut(s) 5, 634, 856
BsuTUI ATCGAT 3 cut(s) 49, 273, 720
BtsCI GGATG 3 cut(s) 382, 523, 846
BtsIMutI CAGTG 3 cut(s) 174, 399, 447
Cac8I GCNNGC 2 cut(s) 183, 647
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 456
Cfr13I GGNCC 3 cut(s) 407, 575, 632
ClaI ATCGAT 3 cut(s) 49, 273, 720
CseI GACGC 1 cut(s) 174
CviAII CATG 3 cut(s) 146, 617, 776
DdeI CTNAG 5 cut(s) 311, 402, 636, 735, 816
DpnI GATC 8 cut(s) 52, 234, 272, 276, 324, 350, 468, 669
DpnII GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
EciI GGCGGA 1 cut(s) 72
Ecl136II GAGCTC 1 cut(s) 335
Eco130I CCWWGG 1 cut(s) 328
Eco24I GRGCYC 1 cut(s) 337
Eco47I GGWCC 2 cut(s) 407, 575
Eco53kI GAGCTC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 486
EcoRII CCWGG 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 328
EcoT22I ATGCAT 1 cut(s) 358
EcoT38I GRGCYC 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 328
FaeI CATG 3 cut(s) 149, 620, 779
FalI AAGNNNNNCTT 2 cut(s) 629, 661
FaqI GGGAC 1 cut(s) 420
FatI CATG 3 cut(s) 145, 616, 775
FauNDI CATATG 1 cut(s) 100
FbaI TGATCA 1 cut(s) 232
FblI GTMKAC 1 cut(s) 835
Fnu4HI GCNGC 1 cut(s) 795
FokI GGATG 3 cut(s) 389, 510, 833
FriOI GRGCYC 1 cut(s) 337
Fsp4HI GCNGC 1 cut(s) 795
FspBI CTAG 3 cut(s) 534, 545, 683
GlaI GCGC 1 cut(s) 455
GluI GCNGC 1 cut(s) 795
GsuI CTGGAG 1 cut(s) 145
HaeIII GGCC 3 cut(s) 5, 634, 856
HapII CCGG 1 cut(s) 850
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 456
Hin1II CATG 3 cut(s) 149, 620, 779
Hin6I GCGC 1 cut(s) 454
HinP1I GCGC 1 cut(s) 454
HincII GTYRAC 1 cut(s) 216
HindII GTYRAC 1 cut(s) 216
HindIII AAGCTT 1 cut(s) 587
HinfI GANTC 5 cut(s) 65, 281, 287, 530, 728
HpaII CCGG 1 cut(s) 850
HphI GGTGA 4 cut(s) 80, 254, 332, 566
Hpy166II GTNNAC 3 cut(s) 216, 829, 836
Hpy188I TCNGA 7 cut(s) 64, 70, 201, 232, 255, 286, 292
Hpy188III TCNNGA 7 cut(s) 162, 236, 278, 470, 527, 578, 850
Hpy8I GTNNAC 3 cut(s) 216, 829, 836
HpyAV CCTTC 5 cut(s) 16, 89, 117, 616, 743
HpyCH4III ACNGT 4 cut(s) 178, 394, 806, 833
HpyCH4IV ACGT 2 cut(s) 437, 838
HpyCH4V TGCA 4 cut(s) 206, 296, 307, 356
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 112
HpyF3I CTNAG 5 cut(s) 311, 402, 636, 735, 816
HpySE526I ACGT 2 cut(s) 437, 838
Hsp92II CATG 3 cut(s) 149, 620, 779
HspAI GCGC 1 cut(s) 454
Kpn2I TCCGGA 1 cut(s) 849
Ksp22I TGATCA 1 cut(s) 232
Kzo9I GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
LmnI GCTCC 3 cut(s) 120, 332, 642
Lsp1109I GCAGC 1 cut(s) 806
LweI GCATC 2 cut(s) 246, 283
MaeI CTAG 3 cut(s) 534, 545, 683
MaeII ACGT 2 cut(s) 437, 838
MaeIII GTNAC 3 cut(s) 394, 839, 875
MalI GATC 8 cut(s) 52, 234, 272, 276, 324, 350, 468, 669
MboI GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
MboII GAAGA 4 cut(s) 24, 476, 595, 638
MflI RGATCY 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 337
MluCI AATT 4 cut(s) 57, 71, 425, 486
MnlI CCTC 8 cut(s) 96, 101, 128, 229, 397, 633, 782, 846
Mph1103I ATGCAT 1 cut(s) 358
MroI TCCGGA 1 cut(s) 849
MseI TTAA 2 cut(s) 714, 800
MslI CAYNNNNRTG 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 734
MspI CCGG 1 cut(s) 850
MspR9I CCNGG 1 cut(s) 675
Mva1269I GAATGC 1 cut(s) 208
MvaI CCWGG 1 cut(s) 675
MwoI GCNNNNNNNGC 2 cut(s) 26, 112
NdeI CATATG 1 cut(s) 100
NdeII GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
NlaIII CATG 3 cut(s) 149, 620, 779
NlaIV GGNNCC 1 cut(s) 408
NsiI ATGCAT 1 cut(s) 358
NspI RCATGY 2 cut(s) 149, 620
PciI ACATGT 1 cut(s) 616
PctI GAATGC 1 cut(s) 208
PfeI GAWTC 5 cut(s) 65, 281, 287, 530, 728
PkrI GCNGC 1 cut(s) 796
PscI ACATGT 1 cut(s) 616
Psp124BI GAGCTC 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 673
PspGI CCWGG 1 cut(s) 673
PspN4I GGNNCC 1 cut(s) 408
PspPI GGNCC 3 cut(s) 407, 575, 632
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 734
RseI CAYNNNNRTG 1 cut(s) 615
SacI GAGCTC 1 cut(s) 337
SaqAI TTAA 2 cut(s) 714, 800
SatI GCNGC 1 cut(s) 795
Sau3AI GATC 8 cut(s) 50, 232, 270, 274, 322, 348, 466, 667
Sau96I GGNCC 3 cut(s) 407, 575, 632
ScrFI CCNGG 1 cut(s) 675
SduI GDGCHC 1 cut(s) 337
SfaNI GCATC 2 cut(s) 246, 283
SfcI CTRYAG 1 cut(s) 784
SinI GGWCC 2 cut(s) 407, 575
SmiMI CAYNNNNRTG 1 cut(s) 615
Sse9I AATT 4 cut(s) 57, 71, 425, 486
SsiI CCGC 1 cut(s) 83
SspMI CTAG 3 cut(s) 534, 545, 683
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 1 cut(s) 673
StyI CCWWGG 1 cut(s) 328
TaaI ACNGT 4 cut(s) 178, 394, 806, 833
TaiI ACGT 2 cut(s) 440, 841
TaqI TCGA 5 cut(s) 49, 273, 490, 670, 720
TasI AATT 4 cut(s) 57, 71, 425, 486
TfiI GAWTC 5 cut(s) 65, 281, 287, 530, 728
Tru1I TTAA 2 cut(s) 714, 800
Tru9I TTAA 2 cut(s) 714, 800
TscAI CASTG 3 cut(s) 181, 406, 454
TseI GCWGC 1 cut(s) 794
TspDTI ATGAA 2 cut(s) 185, 764
TspGWI ACGGA 1 cut(s) 878
TspRI CASTG 3 cut(s) 181, 406, 454
VpaK11BI GGWCC 2 cut(s) 407, 575
XapI RAATTY 4 cut(s) 57, 71, 425, 486
XceI RCATGY 2 cut(s) 149, 620
XmiI GTMKAC 1 cut(s) 835
XspI CTAG 3 cut(s) 534, 545, 683
Zsp2I ATGCAT 1 cut(s) 358
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.