Rh1DG129400

protein DS12 from 2D-PAGE of leaf

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0014353
Physical Location & Seq
Forward (+)
26962294 .. 26968060
5767 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG129400.1

Sequence Viewer

Length: 873 bp
ATGGCCTTCACCAACTCCTCTCTCTTTGCTTCCCATCTCACTCTCCATCGCTCTAAACTTTCCGATTCCGAATTTGCCCCTCCGCCGTGCGAGGCCCTCAAGCTCCTTCACTTTCCTCCCAGCAGCGCCATTCATGCCTGCACTTTCTCCAGAAATTTGAACGTTGTGTGCGCGTCTACCAATGGTGTCAATGCAGTTGGTTCATCGTCGCCGCGGAAGTCTGATCAAGGTGCTGATTATATTCCAATGCCAGTTGTCATGATTGATCAAGATTCAGATTCTGATGCAACTATTGTGCAACTCAGCTTTGGTGATCGTCTCGGAGCACTTATTGACACGATAAATGCATTAAAAGATTTGGGATTGGATGTAGCAAAGGGAACTGTTACCACTGAGGGACCAGTTAAACAGACGAAGTTTCATATAACGCGGTTAGACACCGGGCGCAAAGTTGAAGATCCTGATATGTTAGAGAGAATTCGACTTACCATCATCAACAATCTTTTGAAGTATCATCCAGAATCTAGTCAGCTACTTGCAATGGGTGAAGCTTTTGGAATAAAAGCTCCGGAAAAGAAGCTTGATGTTGATATCGCAACTCATATACACGTGAAGCAAGATGGACCTAAGAGGAGCTTGCTTTATATAGAGACAGCAGATCGACCAGGATTGCTTGTAGAAATTATCAAAATCATTGCCGACATTAATATCGATGTGGAATCAGCTGAGATTGATACAGAAGGTTTGGTTGCTAAAGATAAATTTCATGTCAGCTACAGAGGGGCAGCCTTAAATAGTTCCTTGTCCCAGGTGTTAGTGAACTGTCTGCGTTACTACATTCGCAGGCCAGAAACAGATATAGATAGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

290

Amino Acids

31.8

Weight (kDa)

5.72

Isoelectric Point (pI)

43.42

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014353)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G04740 AT5G04740
fragaria_vesca FvH4_7g05550 FvH4_7g05550
malus_domestica MD07G1063700.v1.1
prunus_persica Prupe.2G077000_v2.0.a1
pyrus_communis pycom07g04910
rosa_chinensis RchiOBHm_Chr1g0332391
rosa_laevigata RLG00000029675
rosa_multiflora Rmu_ssc0000161.1_g000018
rosa_roxburghii Rroxscaffold_4G00318220
rosa_rugosa Rorug01G0100400
rosa_samantha Rh1AG124300 Rh1BG094900 Rh1CG118400 Rh1DG129400
rosa_wichuraiana Rw1G010110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 176
AccII CGCG 3 cut(s) 173, 214, 430
AccIII TCCGGA 1 cut(s) 568
AciI CCGC 4 cut(s) 83, 212, 214, 430
AclI AACGTT 1 cut(s) 162
AclWI GGATC 1 cut(s) 452
AcsI RAATTY 4 cut(s) 71, 154, 477, 761
AcvI CACGTG 1 cut(s) 610
AflIII ACRYGT 1 cut(s) 607
AgsI TTSAA 3 cut(s) 160, 455, 508
AjnI CCWGG 2 cut(s) 664, 807
AluBI AGCT 9 cut(s) 103, 306, 532, 551, 566, 580, 636, 725, 774
AluI AGCT 9 cut(s) 103, 306, 532, 551, 566, 580, 636, 725, 774
Alw21I GWGCWC 1 cut(s) 328
Alw26I GTCTC 2 cut(s) 323, 644
AlwI GGATC 1 cut(s) 452
AlwNI CAGNNNCTG 1 cut(s) 281
Aor13HI TCCGGA 1 cut(s) 568
AoxI GGCC 3 cut(s) 3, 93, 845
ApeKI GCWGC 2 cut(s) 123, 785
ApoI RAATTY 4 cut(s) 71, 154, 477, 761
AseI ATTAAT 1 cut(s) 705
AspLEI GCGC 3 cut(s) 128, 173, 447
AspS9I GGNCC 3 cut(s) 94, 398, 623
AsuC2I CCSGG 1 cut(s) 442
AsuHPI GGTGA 2 cut(s) 323, 557
AvaII GGWCC 2 cut(s) 398, 623
BbrPI CACGTG 1 cut(s) 610
Bbv12I GWGCWC 1 cut(s) 328
BbvI GCAGC 2 cut(s) 135, 797
BccI CCATC 4 cut(s) 42, 54, 497, 614
BceAI ACGGC 1 cut(s) 70
BciT130I CCWGG 2 cut(s) 666, 809
BclI TGATCA 2 cut(s) 223, 265
BcnI CCSGG 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 323, 644
BfaI CTAG 1 cut(s) 525
BfmI CTRYAG 1 cut(s) 775
BfoI RGCGCY 1 cut(s) 129
BisI GCNGC 3 cut(s) 124, 212, 786
BlsI GCNGC 3 cut(s) 125, 213, 787
Bme1390I CCNGG 3 cut(s) 442, 666, 809
Bme18I GGWCC 2 cut(s) 398, 623
BmgT120I GGNCC 3 cut(s) 94, 398, 623
BmiI GGNNCC 1 cut(s) 399
BmrFI CCNGG 3 cut(s) 442, 666, 809
BmsI GCATC 1 cut(s) 274
BpmI CTGGAG 1 cut(s) 133
BpuEI CTTGAG 1 cut(s) 83
BpuMI CCSGG 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 711
BsaAI YACGTR 1 cut(s) 610
BsaJI CCNNGG 2 cut(s) 212, 807
BsaWI WCCGGW 1 cut(s) 568
Bse1I ACTGG 2 cut(s) 251, 401
Bse3DI GCAATG 2 cut(s) 546, 693
BseAI TCCGGA 1 cut(s) 568
BseBI CCWGG 2 cut(s) 666, 809
BseCI ATCGAT 1 cut(s) 711
BseDI CCNNGG 2 cut(s) 212, 807
BseGI GGATG 2 cut(s) 373, 514
BseMI GCAATG 2 cut(s) 546, 693
BseMII CTCAG 3 cut(s) 316, 384, 717
BseNI ACTGG 2 cut(s) 251, 401
BseRI GAGGAG 2 cut(s) 7, 646
BseXI GCAGC 2 cut(s) 135, 797
BseYI CCCAGC 1 cut(s) 119
BsgI GTGCAG 1 cut(s) 124
Bsh1236I CGCG 3 cut(s) 173, 214, 430
BshFI GGCC 3 cut(s) 5, 95, 847
BshVI ATCGAT 1 cut(s) 711
BsiHKAI GWGCWC 1 cut(s) 328
BsiSI CCGG 2 cut(s) 441, 569
BslFI GGGAC 2 cut(s) 411, 790
BsmAI GTCTC 2 cut(s) 323, 644
BsmBI CGTCTC 1 cut(s) 323
BsmFI GGGAC 2 cut(s) 411, 790
BsnI GGCC 3 cut(s) 5, 95, 847
Bsp1286I GDGCHC 1 cut(s) 328
Bsp13I TCCGGA 1 cut(s) 568
Bsp143I GATC 5 cut(s) 223, 265, 313, 457, 658
BspACI CCGC 4 cut(s) 83, 212, 214, 430
BspANI GGCC 3 cut(s) 5, 95, 847
BspCNI CTCAG 3 cut(s) 315, 385, 718
BspDI ATCGAT 1 cut(s) 711
BspEI TCCGGA 1 cut(s) 568
BspFNI CGCG 3 cut(s) 173, 214, 430
BspHI TCATGA 1 cut(s) 258
BspLI GGNNCC 1 cut(s) 399
BspPI GGATC 1 cut(s) 452
BsrDI GCAATG 2 cut(s) 546, 693
BsrI ACTGG 2 cut(s) 251, 401
BssECI CCNNGG 2 cut(s) 212, 807
BssMI GATC 5 cut(s) 223, 265, 313, 457, 658
Bst2UI CCWGG 2 cut(s) 666, 809
Bst4CI ACNGT 2 cut(s) 385, 824
BstBAI YACGTR 1 cut(s) 610
BstC8I GCNNGC 3 cut(s) 139, 638, 845
BstDEI CTNAG 4 cut(s) 302, 393, 627, 726
BstDSI CCRYGG 1 cut(s) 212
BstF5I GGATG 2 cut(s) 373, 514
BstFNI CGCG 3 cut(s) 173, 214, 430
BstH2I RGCGCY 1 cut(s) 129
BstHHI GCGC 3 cut(s) 128, 173, 447
BstKTI GATC 5 cut(s) 226, 268, 316, 460, 661
BstMAI GTCTC 2 cut(s) 323, 644
BstMBI GATC 5 cut(s) 223, 265, 313, 457, 658
BstMWI GCNNNNNNNGC 1 cut(s) 134
BstNI CCWGG 2 cut(s) 666, 809
BstSCI CCNGG 3 cut(s) 440, 664, 807
BstSFI CTRYAG 1 cut(s) 775
BstUI CGCG 3 cut(s) 173, 214, 430
BstV1I GCAGC 2 cut(s) 135, 797
BstX2I RGATCY 1 cut(s) 457
BstYI RGATCY 1 cut(s) 457
Bsu15I ATCGAT 1 cut(s) 711
BsuRI GGCC 3 cut(s) 5, 95, 847
BsuTUI ATCGAT 1 cut(s) 711
BtgI CCRYGG 1 cut(s) 212
BtgZI GCGATG 1 cut(s) 32
BtsCI GGATG 2 cut(s) 373, 514
BtsIMutI CAGTG 1 cut(s) 390
Cac8I GCNNGC 3 cut(s) 139, 638, 845
CaiI CAGNNNCTG 1 cut(s) 281
CciI TCATGA 1 cut(s) 258
CfoI GCGC 3 cut(s) 128, 173, 447
Cfr13I GGNCC 3 cut(s) 94, 398, 623
Cfr42I CCGCGG 1 cut(s) 215
ClaI ATCGAT 1 cut(s) 711
CseI GACGC 1 cut(s) 162
CviAII CATG 3 cut(s) 134, 259, 767
DdeI CTNAG 4 cut(s) 302, 393, 627, 726
DpnI GATC 5 cut(s) 225, 267, 315, 459, 660
DpnII GATC 5 cut(s) 223, 265, 313, 457, 658
EciI GGCGGA 1 cut(s) 72
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 2 cut(s) 398, 623
Eco72I CACGTG 1 cut(s) 610
EcoO109I RGGNCCY 1 cut(s) 94
EcoRI GAATTC 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 664, 807
EcoRV GATATC 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 349
Esp3I CGTCTC 1 cut(s) 323
FaeI CATG 3 cut(s) 137, 262, 770
FalI AAGNNNNNCTT 2 cut(s) 620, 652
FaqI GGGAC 2 cut(s) 411, 790
FatI CATG 3 cut(s) 133, 258, 766
FbaI TGATCA 2 cut(s) 223, 265
FblI GTMKAC 1 cut(s) 176
Fnu4HI GCNGC 3 cut(s) 124, 212, 786
FokI GGATG 2 cut(s) 380, 501
Fsp4HI GCNGC 3 cut(s) 124, 212, 786
FspBI CTAG 1 cut(s) 525
GlaI GCGC 3 cut(s) 127, 172, 446
GluI GCNGC 3 cut(s) 124, 212, 786
GsaI CCCAGC 1 cut(s) 123
GsuI CTGGAG 1 cut(s) 133
HaeII RGCGCY 1 cut(s) 129
HaeIII GGCC 3 cut(s) 5, 95, 847
HapII CCGG 2 cut(s) 441, 569
HgaI GACGC 1 cut(s) 162
HhaI GCGC 3 cut(s) 128, 173, 447
Hin1II CATG 3 cut(s) 137, 262, 770
Hin6I GCGC 3 cut(s) 126, 171, 445
HinP1I GCGC 3 cut(s) 126, 171, 445
HindIII AAGCTT 2 cut(s) 549, 578
HinfI GANTC 5 cut(s) 65, 272, 278, 521, 719
HpaII CCGG 2 cut(s) 441, 569
HphI GGTGA 2 cut(s) 323, 557
Hpy166II GTNNAC 2 cut(s) 177, 820
Hpy188I TCNGA 6 cut(s) 64, 70, 223, 277, 283, 323
Hpy188III TCNNGA 6 cut(s) 150, 259, 269, 461, 518, 569
Hpy8I GTNNAC 2 cut(s) 177, 820
Hpy99I CGWCG 1 cut(s) 211
HpyAV CCTTC 3 cut(s) 16, 116, 734
HpyCH4III ACNGT 2 cut(s) 385, 824
HpyCH4IV ACGT 2 cut(s) 162, 609
HpyCH4V TGCA 6 cut(s) 141, 194, 287, 298, 347, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 134
HpyF3I CTNAG 4 cut(s) 302, 393, 627, 726
HpySE526I ACGT 2 cut(s) 162, 609
Hsp92II CATG 3 cut(s) 137, 262, 770
HspAI GCGC 3 cut(s) 126, 171, 445
Kpn2I TCCGGA 1 cut(s) 568
Ksp22I TGATCA 2 cut(s) 223, 265
KspI CCGCGG 1 cut(s) 215
Kzo9I GATC 5 cut(s) 223, 265, 313, 457, 658
LmnI GCTCC 4 cut(s) 108, 323, 571, 633
Lsp1109I GCAGC 2 cut(s) 135, 797
LweI GCATC 1 cut(s) 274
MaeI CTAG 1 cut(s) 525
MaeII ACGT 2 cut(s) 162, 609
MaeIII GTNAC 3 cut(s) 385, 830, 866
MalI GATC 5 cut(s) 225, 267, 315, 459, 660
MboI GATC 5 cut(s) 223, 265, 313, 457, 658
MboII GAAGA 1 cut(s) 467
MflI RGATCY 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 328
MluCI AATT 5 cut(s) 71, 154, 477, 681, 761
MnlI CCTC 8 cut(s) 28, 85, 90, 107, 126, 388, 624, 773
Mph1103I ATGCAT 1 cut(s) 349
MroI TCCGGA 1 cut(s) 568
MseI TTAA 4 cut(s) 350, 405, 705, 791
MspA1I CMGCKG 2 cut(s) 214, 725
MspI CCGG 2 cut(s) 441, 569
MspR9I CCNGG 3 cut(s) 442, 666, 809
MvaI CCWGG 2 cut(s) 666, 809
MvnI CGCG 3 cut(s) 173, 214, 430
MwoI GCNNNNNNNGC 1 cut(s) 134
NciI CCSGG 1 cut(s) 442
NdeII GATC 5 cut(s) 223, 265, 313, 457, 658
NlaIII CATG 3 cut(s) 137, 262, 770
NlaIV GGNNCC 1 cut(s) 399
NsiI ATGCAT 1 cut(s) 349
PagI TCATGA 1 cut(s) 258
PfeI GAWTC 5 cut(s) 65, 272, 278, 521, 719
PkrI GCNGC 3 cut(s) 125, 213, 787
PmaCI CACGTG 1 cut(s) 610
PmlI CACGTG 1 cut(s) 610
Ppu21I YACGTR 1 cut(s) 610
PshBI ATTAAT 1 cut(s) 705
Psp1406I AACGTT 1 cut(s) 162
Psp6I CCWGG 2 cut(s) 664, 807
PspCI CACGTG 1 cut(s) 610
PspFI CCCAGC 1 cut(s) 119
PspGI CCWGG 2 cut(s) 664, 807
PspN4I GGNNCC 1 cut(s) 399
PspPI GGNCC 3 cut(s) 94, 398, 623
PstNI CAGNNNCTG 1 cut(s) 281
PsuI RGATCY 1 cut(s) 457
PvuII CAGCTG 1 cut(s) 725
SacII CCGCGG 1 cut(s) 215
SaqAI TTAA 4 cut(s) 350, 405, 705, 791
SatI GCNGC 3 cut(s) 124, 212, 786
Sau3AI GATC 5 cut(s) 223, 265, 313, 457, 658
Sau96I GGNCC 3 cut(s) 94, 398, 623
ScrFI CCNGG 3 cut(s) 442, 666, 809
SduI GDGCHC 1 cut(s) 328
SfaNI GCATC 1 cut(s) 274
SfcI CTRYAG 1 cut(s) 775
Sfr303I CCGCGG 1 cut(s) 215
SgrBI CCGCGG 1 cut(s) 215
SinI GGWCC 2 cut(s) 398, 623
SmlI CTYRAG 1 cut(s) 98
SmoI CTYRAG 1 cut(s) 98
Sse9I AATT 5 cut(s) 71, 154, 477, 681, 761
SsiI CCGC 4 cut(s) 83, 212, 214, 430
SspMI CTAG 1 cut(s) 525
StyD4I CCNGG 3 cut(s) 440, 664, 807
TaaI ACNGT 2 cut(s) 385, 824
TaiI ACGT 2 cut(s) 165, 612
TaqI TCGA 3 cut(s) 481, 661, 711
TasI AATT 5 cut(s) 71, 154, 477, 681, 761
TauI GCSGC 1 cut(s) 214
TfiI GAWTC 5 cut(s) 65, 272, 278, 521, 719
Tru1I TTAA 4 cut(s) 350, 405, 705, 791
Tru9I TTAA 4 cut(s) 350, 405, 705, 791
TscAI CASTG 1 cut(s) 397
TseI GCWGC 2 cut(s) 123, 785
TspDTI ATGAA 4 cut(s) 122, 192, 410, 755
TspRI CASTG 1 cut(s) 397
VpaK11BI GGWCC 2 cut(s) 398, 623
VspI ATTAAT 1 cut(s) 705
XapI RAATTY 4 cut(s) 71, 154, 477, 761
XmiI GTMKAC 1 cut(s) 176
XspI CTAG 1 cut(s) 525
Zsp2I ATGCAT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.