Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0014789

Physical Location & Seq

Genomic Coordinates Reverse (-)

6454243 .. 6458964

Gene Structure 4722 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1068200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2931 bp

ATGCTCAATCTCCCTAACAAGTACTTGCATGTCTTCCCTTTGCTTGCTCAGTTCTCACATGAAGAACAAGCATCTTTAGTCTGGAAGATTTTGTGCAGCATCCCTACTAATATAGTTTCAAAGTTCCTTCCCTGGTTGTCGTCCTCTATTTCGCCTGATGAATATCACGATCTGCAAAAGTATTTGAGGACTATAGTTCGAGAGGATAATCTTGTTGAACAAGTTATTTTCGGCTGGATGGAAGGGAAAGATTATTGTGCTAGCATGATATCAAATCAGACAGACGAAACAGACTTTGCATCTGAGTTCTCTGGCGAACATCCAATAGATACTGTAATTCTTTGGCACAATGCTATTAAACGAGAGTTGCATGCGATACTCGAGGAGGCCAAGAAGAATAGTATTTCTGGTGCTGTTACTTATGATCTATTGGCTTTCCATAAGAAGCTACAATTTGTTGCCGAAATTTGTATCTTCCACAGTGTTGCTGAGACAAAAGTCATGTATCCCGCAGTATATGGAGAAACCTTATCTTTTCAGCAGCGCACAAAAGAAGAAAGTTTATGCAAAGAGTTTATGTGTTTGATTGAAAGTATACAGAAGGCAGGAACAATCTCTTCTATTACAGAATTTAATTCATCGATAGCCTCACATGCTGATCGAATAATAGAAACTTTAAAGGGGCATTTCGACAATGAGGAAGTTCAGGTCCTTCCACTTGTGCGAAAGCTCCTAAGCGTCAAAAGACAACGGGAACTTTTGCATCAAAGCTTGTGTGTGATGCCCTTGAAATTAATTGAGCGTGCGTTGCCATGGATGGTAAACTCATTGACTGCAAATGAAGCCAACAACTTTGTTAAAAACATGCAGCTGGCAGCTCCAGCATCAGATATTGCTCTGGTCGAGATCTTTTGTGGTTGGGCTAGCAAGCCTCTCAACGACAGCTCCTGCTTGAGTGCAAGCGGGCGCTGTCCTGTCAGAAGGTTCGTTAGTAGTGATGAGAAAATTGGTCAATTATCACGTGCTTGGTGTGCTTCCACATTGTCAAGCAGAGACACCTACTCCTCTCCATATCAAGAGAGCAATGCTTCTCACCCTTTACAACCAATTGATGTTGTTTTTAAAGTTCATAAGGCCATATGCCGTGACTTGGAATATCTTGACAATGAATCGGAAAAAGTCAGCACTTGTGATGAGATATTTCTTCAGCAGTTCAGTGGATTTTTCTGTTTCTTATGGGACTTATCCAGAGCTCATAGTAATGCTGAGGACAATATAGTGTATCCGGCACTGGAATCCCGAGATGCACTTTACAATGTGAGTCACTCATTCACACTGGATCATGAGCAAGAACAACTGGCATTTGAAAATATTTCTGGTCTTCTTTTTGAGCTTTCACACCTTCACGGAAGTGTGATAGCTTTTTCTGGTACTTGTGGTGGTGAGTCAAGCGAATGCTTGAGAAAGTACCACGAATTAGCTATCAGGCTTCGTGTATCGCTCAAGTCACTAAGAATTATGGTAGACCAGCACATGACAAGGGAAGAGATTGAACTGTGGCCACTATTTGGATTGCACTTCTCTGTGGAGGATCAAAATAAAATGGTTGGTTTCATACTGGGGACTACAGGTGCTGACGTGCTAAAAACTATGTTACCCTGGGTAACTTCAGCACTTACCGAGGCTGAACAGAGTGAAATGATGAATACCTTTAATCAGGTGACCAAAAATACAATGTTCAATGAATGGCTAAATGAAAGCTCTAACGGAAATTCATTCTCAAGTTCACAGACTCGGAGGCTTGAAACTCGCATTTCTCCAAAAGGAAGCGTGCGGCAAATTGATCGTGCCTTCAACTCTCATAAGAAGCATAACTTGAAGAATCAAATGTCTAGTTACTGGATGGCTGCACAGCAGAGTTTTCCCCGAGCTTCAGCAGTAAATAATTGTGAGGGTGAAGATTTAGTGGGACGGTCACCAACATTTCAAGATTCCGAAAAAGGAGTATATGGGTGTAAGCATTACAAAAGAAACTGCAAACTCCATGCCACTTGCTGCAACAAGCTGTTTACATGTAGATATTGCCATGATATTGGCAGTGACCACAAAATGGATTGGAAACAAACGTCGGAAATGATGTGTATGCGCTGCCTCAATATTCAGCCAGTCGGGCCAATATGCTCCACGCCTTCTTGCAACGGACTCTCAATGGCAAAATATTATTGTAATTCATGCAAACTTTTTGATGATGGCAGGAATGTTTATCATTGTCCATTTTGCAATTTATGCCGTGTTGGGAAGGGCCTTGGCATCGACTATTTTCACTGCATGAAGTGCAATTGTTGCTTGAGCATAAGTATAGTGAACCACAAGTGCCGGGAGAAATGCCTCGAAGCTAACTGCCCCATCTGCAATGACTTCTTGTTCACATCAACCTCAAGCATCAGAGCTTTACCATGTGGCCATTTGATGCATGTAGTTTGCTTGGAGGCTTCCTCTCGTAGTCATTACAGTATATGTCCCATCTGCTGCAAATCTTCCGGAAATATGATGAGGGCATCAACTCAGTCTTCCATTCTGGTTGGTGGTGATGAATCCATGGAATTTTCCGAATGTCATGAAAGAACCCAAACGAAGACTTCACGGACTAAGAAAAAACTCGAAGGAATATTTTCCAGCCACGCGAGTTTTTTGGTAAATACGTTATATCATTCTGTGCCATGGTTTGAACCCCATACCAGCAAGCATCCTCGACTTCTGCACCGATTGAAGAAGGGGTTGGAGAATGCGGTTGCAGGACAAAATGGCAAGGAGAGGTTCCTGATGTTTTTTCTGGTTCTGTGCATTCTGGTGCTCTTTTGTGGCTTAAGCAGTGTTGTAATTTTACTTTGGTTCAAGGCATTGGGGAGCAACAAGTACAGTTACCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

977

Amino Acids

110.64

Weight (kDa)

6.96

Isoelectric Point (pI)

48.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hemerythrin	PF01814	369 - 519	1.1e-06	Hemerythrin HHE cation binding domain
zf-CHY	PF05495	672 - 740	2.5e-15	CHY zinc finger
zf-RING_2	PF13639	800 - 843	7.2e-07	Ring finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014789)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g05930 FvH4_7g05930 FvH4_7g05930
malus_domestica	MD07G1068200.v1.1
prunus_persica	Prupe.2G085600_v2.0.a1
pyrus_communis	pycom07g05230
rosa_chinensis	RchiOBHm_Chr1g0333131
rosa_laevigata	RLG00000029624
rosa_multiflora	Rmu_sc0007432.1_g000001
rosa_roxburghii	Rroxscaffold_4G00317770
rosa_rugosa	Rorug01G0107300 Rorug01G0107400
rosa_samantha	Rh1AG130600 Rh1BG099700 Rh1CG124400 Rh1DG135900
rosa_wichuraiana	Rw1G010240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	1568, 2110
AccI	GTMKAC	2 cut(s)	595, 1524
AccII	CGCG	1 cut(s)	2684
AccIII	TCCGGA	1 cut(s)	2540
AciI	CCGC	4 cut(s)	510, 963, 1834, 2789
AclWI	GGATC	2 cut(s)	1347, 1599
AcoI	YGGCCR	2 cut(s)	1559, 2461
AcsI	RAATTY	4 cut(s)	465, 629, 1771, 2603
AcuI	CTGAAG	3 cut(s)	1190, 1653, 1917
AcvI	CACGTG	1 cut(s)	1022
AfaI	GTAC	4 cut(s)	23, 1432, 1469, 2918
AfiI	CCNNNNNNNGG	3 cut(s)	1150, 1568, 2110
AflII	CTTAAG	1 cut(s)	2866
AflIII	ACRYGT	1 cut(s)	2072
AjiI	CACGTC	1 cut(s)	1639
AjnI	CCWGG	2 cut(s)	131, 1658
AjuI	GAANNNNNNNTTGG	2 cut(s)	2762, 2794
AleI	CACNNNNGTG	1 cut(s)	1410
Alw21I	GWGCWC	2 cut(s)	1255, 2856
Alw26I	GTCTC	2 cut(s)	485, 1047
AlwI	GGATC	2 cut(s)	1347, 1599
AlwNI	CAGNNNCTG	2 cut(s)	948, 1634
Ama87I	CYCGRG	3 cut(s)	380, 1299, 1926
Aor13HI	TCCGGA	1 cut(s)	2540
AoxI	GGCC	6 cut(s)	387, 1134, 1559, 2171, 2302, 2461
ApeKI	GCWGC	8 cut(s)	96, 541, 868, 875, 1907, 2055, 2148, 2529
ApoI	RAATTY	4 cut(s)	465, 629, 1771, 2603
AseI	ATTAAT	1 cut(s)	794
Asp700I	GAANNNNTTC	1 cut(s)	2671
AspLEI	GCGC	3 cut(s)	546, 969, 2148
AspS9I	GGNCC	3 cut(s)	709, 2171, 2302
AsuC2I	CCSGG	1 cut(s)	2378
AsuHPI	GGTGA	6 cut(s)	1085, 1454, 1732, 1967, 1968, 2600
AsuNHI	GCTAGC	2 cut(s)	260, 923
AvaI	CYCGRG	3 cut(s)	380, 1299, 1926
AvaII	GGWCC	1 cut(s)	709
BalI	TGGCCA	2 cut(s)	1561, 2463
BanII	GRGCYC	1 cut(s)	1255
BbrPI	CACGTG	1 cut(s)	1022
BbsI	GAAGAC	4 cut(s)	25, 1373, 2562, 2642
Bbv12I	GWGCWC	2 cut(s)	1255, 2856
BbvCI	CCTCAGC	1 cut(s)	1266
BbvI	GCAGC	8 cut(s)	108, 553, 880, 887, 1894, 2042, 2135, 2516
BccI	CCATC	6 cut(s)	232, 811, 1897, 2243, 2414, 2531
BceAI	ACGGC	2 cut(s)	1128, 2274
BcgI	CGANNNNNNTGC	2 cut(s)	1826, 1860
BciT130I	CCWGG	2 cut(s)	133, 1660
BciVI	GTATCC	2 cut(s)	516, 1293
BcnI	CCSGG	1 cut(s)	2378
BcoDI	GTCTC	2 cut(s)	485, 1047
BfaI	CTAG	3 cut(s)	261, 924, 1893
BfmI	CTRYAG	2 cut(s)	192, 1626
BfoI	RGCGCY	1 cut(s)	970
BfrI	CTTAAG	1 cut(s)	2866
BfuI	GTATCC	2 cut(s)	516, 1293
BglI	GCCNNNNNGGC	1 cut(s)	2170
BglII	AGATCT	1 cut(s)	906
BisI	GCNGC	9 cut(s)	97, 542, 869, 876, 1835, 1908, 2056, 2149, 2530
BlsI	GCNGC	9 cut(s)	98, 543, 870, 877, 1836, 1909, 2057, 2150, 2531
BmcAI	AGTACT	1 cut(s)	23
Bme1390I	CCNGG	3 cut(s)	133, 1660, 2378
Bme18I	GGWCC	1 cut(s)	709
BmeT110I	CYCGRG	3 cut(s)	380, 1299, 1926
BmgBI	CACGTC	1 cut(s)	1639
BmgT120I	GGNCC	3 cut(s)	709, 2171, 2302
BmiI	GGNNCC	1 cut(s)	2819
BmrFI	CCNGG	3 cut(s)	133, 1660, 2378
BmrI	ACTGGG	1 cut(s)	1628
BmtI	GCTAGC	2 cut(s)	264, 927
BmuI	ACTGGG	1 cut(s)	1628
BoxI	GACNNNNGTC	1 cut(s)	497
BpiI	GAAGAC	4 cut(s)	25, 1373, 2562, 2642
BplI	GAGNNNNNCTC	2 cut(s)	2480, 2512
BpmI	CTGGAG	1 cut(s)	864
Bpu10I	CCTNAGC	2 cut(s)	734, 1266
BpuEI	CTTGAG	6 cut(s)	973, 1480, 1487, 1765, 2368, 2422
BpuMI	CCSGG	1 cut(s)	2378
Bsa29I	ATCGAT	1 cut(s)	641
BsaAI	YACGTR	1 cut(s)	1022
BsaBI	GATNNNNATC	1 cut(s)	162
BsaJI	CCNNGG	8 cut(s)	131, 812, 1658, 1659, 1680, 2305, 2598, 2720
BsaWI	WCCGGW	1 cut(s)	2540
BsaXI	ACNNNNNCTCC	4 cut(s)	929, 959, 1046, 1076
Bsc4I	CCNNNNNNNGG	3 cut(s)	1150, 1568, 2110
Bse1I	ACTGG	6 cut(s)	1296, 1341, 1362, 1623, 1904, 2165
Bse3DI	GCAATG	2 cut(s)	1090, 2419
Bse8I	GATNNNNATC	1 cut(s)	162
BseAI	TCCGGA	1 cut(s)	2540
BseBI	CCWGG	2 cut(s)	133, 1660
BseCI	ATCGAT	1 cut(s)	641
BseDI	CCNNGG	8 cut(s)	131, 812, 1658, 1659, 1680, 2305, 2598, 2720
BseGI	GGATG	6 cut(s)	99, 243, 319, 822, 1908, 2746
BseJI	GATNNNNATC	1 cut(s)	162
BseLI	CCNNNNNNNGG	3 cut(s)	1150, 1568, 2110
BseMI	GCAATG	2 cut(s)	1090, 2419
BseMII	CTCAG	5 cut(s)	62, 294, 480, 1257, 2579
BseNI	ACTGG	6 cut(s)	1296, 1341, 1362, 1623, 1904, 2165
BseRI	GAGGAG	2 cut(s)	398, 1054
BseXI	GCAGC	8 cut(s)	108, 553, 880, 887, 1894, 2042, 2135, 2516
BsgI	GTGCAG	3 cut(s)	115, 1893, 2744
Bsh1236I	CGCG	1 cut(s)	2684
BshFI	GGCC	6 cut(s)	389, 1136, 1561, 2173, 2304, 2463
BshVI	ATCGAT	1 cut(s)	641
BsiHKAI	GWGCWC	2 cut(s)	1255, 2856
BsiHKCI	CYCGRG	3 cut(s)	380, 1299, 1926
BsiSI	CCGG	3 cut(s)	1286, 2377, 2541
BslFI	GGGAC	4 cut(s)	1253, 1636, 1983, 2505
BslI	CCNNNNNNNGG	3 cut(s)	1150, 1568, 2110
BsmAI	GTCTC	2 cut(s)	485, 1047
BsmFI	GGGAC	4 cut(s)	1253, 1636, 1983, 2505
BsmI	GAATGC	3 cut(s)	1460, 2791, 2844
BsnI	GGCC	6 cut(s)	389, 1136, 1561, 2173, 2304, 2463
BsoBI	CYCGRG	3 cut(s)	380, 1299, 1926
Bsp1286I	GDGCHC	2 cut(s)	1255, 2856
Bsp13I	TCCGGA	1 cut(s)	2540
Bsp143I	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
Bsp19I	CCATGG	3 cut(s)	812, 2598, 2720
BspACI	CCGC	4 cut(s)	510, 963, 1834, 2789
BspANI	GGCC	6 cut(s)	389, 1136, 1561, 2173, 2304, 2463
BspCNI	CTCAG	5 cut(s)	61, 295, 481, 1258, 2578
BspDI	ATCGAT	1 cut(s)	641
BspEI	TCCGGA	1 cut(s)	2540
BspFNI	CGCG	1 cut(s)	2684
BspHI	TCATGA	2 cut(s)	1342, 2617
BspLI	GGNNCC	1 cut(s)	2819
BspOI	GCTAGC	2 cut(s)	264, 927
BspPI	GGATC	2 cut(s)	1347, 1599
BspTI	CTTAAG	1 cut(s)	2866
BsrDI	GCAATG	2 cut(s)	1090, 2419
BsrI	ACTGG	6 cut(s)	1296, 1341, 1362, 1623, 1904, 2165
BssECI	CCNNGG	8 cut(s)	131, 812, 1658, 1659, 1680, 2305, 2598, 2720
BssMI	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
BssNAI	GTATAC	1 cut(s)	596
BssT1I	CCWWGG	4 cut(s)	812, 2305, 2598, 2720
Bst1107I	GTATAC	1 cut(s)	596
Bst2UI	CCWGG	2 cut(s)	133, 1660
Bst4CI	ACNGT	6 cut(s)	334, 482, 1557, 1974, 2513, 2921
Bst6I	CTCTTC	2 cut(s)	622, 1539
BstAFI	CTTAAG	1 cut(s)	2866
BstAPI	GCANNNNNTGC	3 cut(s)	2286, 2334, 2343
BstBAI	YACGTR	1 cut(s)	1022
BstDEI	CTNAG	8 cut(s)	48, 303, 489, 734, 1266, 1511, 2565, 2649
BstDSI	CCRYGG	3 cut(s)	812, 2598, 2720
BstEII	GGTNACC	2 cut(s)	1720, 1974
BstF5I	GGATG	6 cut(s)	99, 243, 319, 822, 1908, 2746
BstFNI	CGCG	1 cut(s)	2684
BstH2I	RGCGCY	1 cut(s)	970
BstHHI	GCGC	3 cut(s)	546, 969, 2148
BstKTI	GATC	7 cut(s)	172, 427, 661, 909, 1342, 1594, 1846
BstMAI	GTCTC	2 cut(s)	485, 1047
BstMBI	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
BstNI	CCWGG	2 cut(s)	133, 1660
BstNSI	RCATGY	6 cut(s)	32, 374, 656, 868, 2076, 2477
BstPAI	GACNNNNGTC	1 cut(s)	497
BstPI	GGTNACC	2 cut(s)	1720, 1974
BstSCI	CCNGG	3 cut(s)	131, 1658, 2376
BstSFI	CTRYAG	2 cut(s)	192, 1626
BstUI	CGCG	1 cut(s)	2684
BstV1I	GCAGC	8 cut(s)	108, 553, 880, 887, 1894, 2042, 2135, 2516
BstV2I	GAAGAC	4 cut(s)	25, 1373, 2562, 2642
BstX2I	RGATCY	1 cut(s)	906
BstXI	CCANNNNNNTGG	1 cut(s)	2093
BstYI	RGATCY	1 cut(s)	906
BstZ17I	GTATAC	1 cut(s)	596
Bsu15I	ATCGAT	1 cut(s)	641
BsuI	GTATCC	2 cut(s)	516, 1293
BsuRI	GGCC	6 cut(s)	389, 1136, 1561, 2173, 2304, 2463
BsuTUI	ATCGAT	1 cut(s)	641
BtgI	CCRYGG	3 cut(s)	812, 2598, 2720
BtrI	CACGTC	1 cut(s)	1639
BtsCI	GGATG	6 cut(s)	99, 243, 319, 822, 1908, 2746
BtsI	GCAGTG	3 cut(s)	2104, 2323, 2878
BtsIMutI	CAGTG	7 cut(s)	487, 1222, 1289, 1334, 2104, 2323, 2878
CaiI	CAGNNNCTG	2 cut(s)	948, 1634
CciI	TCATGA	2 cut(s)	1342, 2617
CfoI	GCGC	3 cut(s)	546, 969, 2148
Cfr13I	GGNCC	3 cut(s)	709, 2171, 2302
ClaI	ATCGAT	1 cut(s)	641
CseI	GACGC	1 cut(s)	727
Csp6I	GTAC	4 cut(s)	22, 1431, 1468, 2917
CviQI	GTAC	4 cut(s)	22, 1431, 1468, 2917
DdeI	CTNAG	8 cut(s)	48, 303, 489, 734, 1266, 1511, 2565, 2649
DpnI	GATC	7 cut(s)	171, 426, 660, 908, 1341, 1593, 1845
DpnII	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
DraI	TTTAAA	2 cut(s)	678, 1123
EaeI	YGGCCR	2 cut(s)	1559, 2461
Eam1104I	CTCTTC	2 cut(s)	622, 1539
EarI	CTCTTC	2 cut(s)	622, 1539
Ecl136II	GAGCTC	1 cut(s)	1253
Eco130I	CCWWGG	4 cut(s)	812, 2305, 2598, 2720
Eco24I	GRGCYC	1 cut(s)	1255
Eco32I	GATATC	1 cut(s)	270
Eco47I	GGWCC	1 cut(s)	709
Eco53kI	GAGCTC	1 cut(s)	1253
Eco57I	CTGAAG	3 cut(s)	1190, 1653, 1917
Eco72I	CACGTG	1 cut(s)	1022
Eco88I	CYCGRG	3 cut(s)	380, 1299, 1926
Eco91I	GGTNACC	2 cut(s)	1720, 1974
EcoICRI	GAGCTC	1 cut(s)	1253
EcoO109I	RGGNCCY	2 cut(s)	709, 2302
EcoO65I	GGTNACC	2 cut(s)	1720, 1974
EcoRII	CCWGG	2 cut(s)	131, 1658
EcoRV	GATATC	1 cut(s)	270
EcoT14I	CCWWGG	4 cut(s)	812, 2305, 2598, 2720
EcoT22I	ATGCAT	1 cut(s)	2475
EcoT38I	GRGCYC	1 cut(s)	1255
ErhI	CCWWGG	4 cut(s)	812, 2305, 2598, 2720
FalI	AAGNNNNNCTT	2 cut(s)	1859, 1891
FaqI	GGGAC	4 cut(s)	1253, 1636, 1983, 2505
FauI	CCCGC	2 cut(s)	517, 956
FauNDI	CATATG	1 cut(s)	1139
FblI	GTMKAC	2 cut(s)	595, 1524
Fnu4HI	GCNGC	9 cut(s)	97, 542, 869, 876, 1835, 1908, 2056, 2149, 2530
FokI	GGATG	6 cut(s)	86, 250, 306, 829, 1915, 2733
FriOI	GRGCYC	1 cut(s)	1255
Fsp4HI	GCNGC	9 cut(s)	97, 542, 869, 876, 1835, 1908, 2056, 2149, 2530
FspBI	CTAG	3 cut(s)	261, 924, 1893
GlaI	GCGC	3 cut(s)	545, 968, 2147
GluI	GCNGC	9 cut(s)	97, 542, 869, 876, 1835, 1908, 2056, 2149, 2530
GsuI	CTGGAG	1 cut(s)	864
HaeII	RGCGCY	1 cut(s)	970
HaeIII	GGCC	6 cut(s)	389, 1136, 1561, 2173, 2304, 2463
HapII	CCGG	3 cut(s)	1286, 2377, 2541
HgaI	GACGC	1 cut(s)	727
HhaI	GCGC	3 cut(s)	546, 969, 2148
Hin6I	GCGC	3 cut(s)	544, 967, 2146
HinP1I	GCGC	3 cut(s)	544, 967, 2146
HindIII	AAGCTT	1 cut(s)	769
HinfI	GANTC	9 cut(s)	1169, 1295, 1321, 1445, 1792, 1882, 1991, 2202, 2594
HpaII	CCGG	3 cut(s)	1286, 2377, 2541
HphI	GGTGA	6 cut(s)	1085, 1454, 1732, 1967, 1968, 2600
Hpy166II	GTNNAC	7 cut(s)	596, 823, 1525, 1787, 2070, 2365, 2427
Hpy8I	GTNNAC	7 cut(s)	596, 823, 1525, 1787, 2070, 2365, 2427
Hpy99I	CGWCG	1 cut(s)	2131
HpyCH4III	ACNGT	6 cut(s)	334, 482, 1557, 1974, 2513, 2921
HpyCH4IV	ACGT	4 cut(s)	1021, 1638, 2126, 2702
HpyF3I	CTNAG	8 cut(s)	48, 303, 489, 734, 1266, 1511, 2565, 2649
HpySE526I	ACGT	4 cut(s)	1021, 1638, 2126, 2702
HspAI	GCGC	3 cut(s)	544, 967, 2146
Kpn2I	TCCGGA	1 cut(s)	2540
Kzo9I	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
LmnI	GCTCC	5 cut(s)	735, 883, 950, 2186, 2907
Lsp1109I	GCAGC	8 cut(s)	108, 553, 880, 887, 1894, 2042, 2135, 2516
MaeI	CTAG	3 cut(s)	261, 924, 1893
MaeII	ACGT	4 cut(s)	1021, 1638, 2126, 2702
MalI	GATC	7 cut(s)	171, 426, 660, 908, 1341, 1593, 1845
MboI	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
MfeI	CAATTG	2 cut(s)	1107, 2338
MflI	RGATCY	1 cut(s)	906
MhlI	GDGCHC	2 cut(s)	1255, 2856
MlsI	TGGCCA	2 cut(s)	1561, 2463
MluNI	TGGCCA	2 cut(s)	1561, 2463
MlyI	GAGTC	4 cut(s)	1330, 1454, 1786, 2196
MmeI	TCCRAC	2 cut(s)	2109, 2760
Mox20I	TGGCCA	2 cut(s)	1561, 2463
Mph1103I	ATGCAT	1 cut(s)	2475
MroI	TCCGGA	1 cut(s)	2540
MroXI	GAANNNNTTC	1 cut(s)	2671
MscI	TGGCCA	2 cut(s)	1561, 2463
MseI	TTAA	8 cut(s)	357, 633, 677, 794, 858, 1122, 1713, 2867
MslI	CAYNNNNRTG	3 cut(s)	1260, 1410, 2849
Msp20I	TGGCCA	2 cut(s)	1561, 2463
MspA1I	CMGCKG	1 cut(s)	871
MspCI	CTTAAG	1 cut(s)	2866
MspI	CCGG	3 cut(s)	1286, 2377, 2541
MspR9I	CCNGG	3 cut(s)	133, 1660, 2378
MunI	CAATTG	2 cut(s)	1107, 2338
Mva1269I	GAATGC	3 cut(s)	1460, 2791, 2844
MvaI	CCWGG	2 cut(s)	133, 1660
MvnI	CGCG	1 cut(s)	2684
NciI	CCSGG	1 cut(s)	2378
NcoI	CCATGG	3 cut(s)	812, 2598, 2720
NdeI	CATATG	1 cut(s)	1139
NdeII	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
NheI	GCTAGC	2 cut(s)	260, 923
NlaIV	GGNNCC	1 cut(s)	2819
NmuCI	GTSAC	6 cut(s)	1145, 1322, 1506, 1720, 1974, 2099
NsiI	ATGCAT	1 cut(s)	2475
NspI	RCATGY	6 cut(s)	32, 374, 656, 868, 2076, 2477
OliI	CACNNNNGTG	1 cut(s)	1410
PaeI	GCATGC	1 cut(s)	374
PaeR7I	CTCGAG	1 cut(s)	380
PagI	TCATGA	2 cut(s)	1342, 2617
PasI	CCCWGGG	1 cut(s)	1659
PciI	ACATGT	1 cut(s)	2072
PctI	GAATGC	3 cut(s)	1460, 2791, 2844
PdmI	GAANNNNTTC	1 cut(s)	2671
PfeI	GAWTC	5 cut(s)	1169, 1295, 1882, 1991, 2594
PflMI	CCANNNNNTGG	2 cut(s)	1568, 2110
PkrI	GCNGC	9 cut(s)	98, 543, 870, 877, 1836, 1909, 2057, 2150, 2531
PleI	GAGTC	4 cut(s)	1329, 1453, 1786, 2196
PmaCI	CACGTG	1 cut(s)	1022
PmlI	CACGTG	1 cut(s)	1022
PpsI	GAGTC	4 cut(s)	1329, 1453, 1786, 2196
Ppu21I	YACGTR	1 cut(s)	1022
PpuMI	RGGWCCY	1 cut(s)	709
PscI	ACATGT	1 cut(s)	2072
PshAI	GACNNNNGTC	1 cut(s)	497
PshBI	ATTAAT	1 cut(s)	794
Psp124BI	GAGCTC	1 cut(s)	1255
Psp5II	RGGWCCY	1 cut(s)	709
Psp6I	CCWGG	2 cut(s)	131, 1658
PspCI	CACGTG	1 cut(s)	1022
PspEI	GGTNACC	2 cut(s)	1720, 1974
PspGI	CCWGG	2 cut(s)	131, 1658
PspN4I	GGNNCC	1 cut(s)	2819
PspPI	GGNCC	3 cut(s)	709, 2171, 2302
PspPPI	RGGWCCY	1 cut(s)	709
PspXI	VCTCGAGB	1 cut(s)	380
PstNI	CAGNNNCTG	2 cut(s)	948, 1634
PsuI	RGATCY	1 cut(s)	906
PvuII	CAGCTG	1 cut(s)	871
RsaI	GTAC	4 cut(s)	23, 1432, 1469, 2918
RsaNI	GTAC	4 cut(s)	22, 1431, 1468, 2917
RseI	CAYNNNNRTG	3 cut(s)	1260, 1410, 2849
SacI	GAGCTC	1 cut(s)	1255
SaqAI	TTAA	8 cut(s)	357, 633, 677, 794, 858, 1122, 1713, 2867
SatI	GCNGC	9 cut(s)	97, 542, 869, 876, 1835, 1908, 2056, 2149, 2530
Sau3AI	GATC	7 cut(s)	169, 424, 658, 906, 1339, 1591, 1843
Sau96I	GGNCC	3 cut(s)	709, 2171, 2302
ScaI	AGTACT	1 cut(s)	23
SchI	GAGTC	4 cut(s)	1330, 1454, 1786, 2196
ScrFI	CCNGG	3 cut(s)	133, 1660, 2378
SduI	GDGCHC	2 cut(s)	1255, 2856
SfcI	CTRYAG	2 cut(s)	192, 1626
Sfr274I	CTCGAG	1 cut(s)	380
SinI	GGWCC	1 cut(s)	709
SlaI	CTCGAG	1 cut(s)	380
SmiMI	CAYNNNNRTG	3 cut(s)	1260, 1410, 2849
SmlI	CTYRAG	8 cut(s)	380, 952, 1459, 1502, 1780, 2347, 2437, 2866
SmoI	CTYRAG	8 cut(s)	380, 952, 1459, 1502, 1780, 2347, 2437, 2866
SphI	GCATGC	1 cut(s)	374
SsiI	CCGC	4 cut(s)	510, 963, 1834, 2789
SspI	AATATT	4 cut(s)	1372, 2158, 2219, 2670
SspMI	CTAG	3 cut(s)	261, 924, 1893
SstI	GAGCTC	1 cut(s)	1255
StyD4I	CCNGG	3 cut(s)	131, 1658, 2376
StyI	CCWWGG	4 cut(s)	812, 2305, 2598, 2720
TaaI	ACNGT	6 cut(s)	334, 482, 1557, 1974, 2513, 2921
TaiI	ACGT	4 cut(s)	1024, 1641, 2129, 2705
TatI	WGTACW	2 cut(s)	21, 2916
TauI	GCSGC	1 cut(s)	1837
TfiI	GAWTC	5 cut(s)	1169, 1295, 1882, 1991, 2594
Tru1I	TTAA	8 cut(s)	357, 633, 677, 794, 858, 1122, 1713, 2867
Tru9I	TTAA	8 cut(s)	357, 633, 677, 794, 858, 1122, 1713, 2867
TscAI	CASTG	7 cut(s)	487, 1222, 1296, 1341, 2104, 2330, 2878
TseFI	GTSAC	6 cut(s)	1145, 1322, 1506, 1720, 1974, 2099
TseI	GCWGC	8 cut(s)	96, 541, 868, 875, 1907, 2055, 2148, 2529
Tsp45I	GTSAC	6 cut(s)	1145, 1322, 1506, 1720, 1974, 2099
TspGWI	ACGGA	4 cut(s)	1422, 1782, 2214, 2659
TspRI	CASTG	7 cut(s)	487, 1222, 1296, 1341, 2104, 2330, 2878
Van91I	CCANNNNNTGG	2 cut(s)	1568, 2110
Vha464I	CTTAAG	1 cut(s)	2866
VpaK11BI	GGWCC	1 cut(s)	709
VspI	ATTAAT	1 cut(s)	794
XapI	RAATTY	4 cut(s)	465, 629, 1771, 2603
XceI	RCATGY	6 cut(s)	32, 374, 656, 868, 2076, 2477
XhoI	CTCGAG	1 cut(s)	380
XmiI	GTMKAC	2 cut(s)	595, 1524
XmnI	GAANNNNTTC	1 cut(s)	2671
XspI	CTAG	3 cut(s)	261, 924, 1893
ZrmI	AGTACT	1 cut(s)	23
Zsp2I	ATGCAT	1 cut(s)	2475

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.