Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0014789

Physical Location & Seq

Genomic Coordinates Reverse (-)

28483561 .. 28488800

Gene Structure 5240 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG135900.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2427 bp

ATGATAGTACCAGCCGAGAAGCTTCTCCAAGAGGTCATTTTCACCTGGATGGAAGGGAAAGACAATTGTGTTGGCATGACAGCTGATCGAAAAGATGATACAAGTTGTGCATATGAGTTCAATGGGGTGTATCCAATAGATGATATGTTGCTTTGGCATCATGCTATTGAGACAGAGTTGAATGAAACACTTGCGGAAGAGAAGAAGATGTCTATTTCTGGAGAAGTTATTAATTATGATTTACTGGCTTTCTATGACAGGCTGCAATTTATTGCTGAAATTTGCATGTTTCACAGTATTGCTGAGGAAACGGTTCTATATCCTGCAGTGTATGGAGAAATTCCAATTTTCCATGAGCACAGAAATGAAGTAAATCTAACCAATGAGTTTAGGTATTTGCTCGAAAGTTTAAAAAGCGCATCAACAATTCCTTCCACAGCTGCTGAATTTCATTCAAAGATATGCTCCCGTGCTGATCAAATAATGAAAACTTTAAAGGCGCATTTCCATGATGAGGAAGTTAAGGTACTTCCACTTGTTCGAAAGCACTTGAGCATTAAAAGACAACAGGAACTTCTGCATCAGAGCTTGTGTGTGATGCCCTTGAAACTAATTGAGCGTGTCTTGCCATGGCTGGTAAAGTCGTTGACTGCAAATGAAGCCCAGAATTTTGTCAATAACATGCAGTTGGCAGCTCCAGCATCAGATATCGCACTGGTGCAGCTCTTTTGTGGTTGGGCTAGTAAGGCTTCTAATGACTGCTTATGCTCATCTATTTCCTTGCATATCAACTCTATATCACCATCACATTCAGTTTGTGAAAAATCCAATAGCTGTTCTACCACTGGAATACCTGCACAACCAATAGATGTTGTATTTAAAGTTCATAAGGCCGTACGCCGTGACTTGGAATATCTAGACACTGAATCTGAAAGGCTTAGTAACTGTGATGAAATATTTCTTCAGCAGTTCATTGAAAGTTTCTGTTTGTTATGGGGCTTATATAGAGCTCATAGTAATGCAGAGGATTTTGTATTGTATCCGGCAATGGAATCCAGAGACACATTTCAAAATGTAAGTCACTCCTACACACTTGATCATAAGCAAGAGGAAGATGCATTTGAAAATATTTCAGGTGTTCTTCTTGAGCTTTCTCACATTCACAGAGGTACAAGATCTGGTGCCCCCATAAGTGAATGTGTGGGAAAGTACTATGAGCTGGCAAACAAGCTTCGAATAATGTTCATGTCGCTAAGAATGATGGTAGATGAGCACATGTACCGGGAAGAGCTTGAGCTGTGGCCATTATTTGGAGCACACTTCTCTGTGGAGGAGCAAAACAAAATGATCGGTTTCATACTGGGGACTACAGGTGCTGAAGTTCTCCAATCTATGTTACCATGGGTTACTTCTGCACTTACTGAGGATGAACGGAGTAAAATGACGGAGACCTTCAAGAATGTTACAAAAAATACAATGTTCAAGGAGTGGCTTAGTGAATGCGGGAAAGGAAGCTCACTGTCAACTCTACCGCCTAAACCGGAAACTAGCATTTTCATAAAAGGTACTGGATTTGAAGAAAGCCATCACCAAATTGATCATACTTTCAACTCTCATACGCGGAATAACTTGAAGCATCAAATGACTAGTTACTGGATTGCTGCTCAGCAGAGTTTAACTATAGCAATAACAGTGGATAGTTCAAATAGTGAACAGTTAGGGGGACTGTCACCAACGTTTCGAGATCATGAGAAAACAGTGTATGGCTGCCAGCATTACAAGAGAAACTGCAAACTCCTAGCTGCTTGCTGCGGCAAACTGTTCACTTGTAGATTTTGCCATGACATTATGAGCGACCACCCAATGGATAGGAAAGCAACATCCAAAATGATGTGTATGAGCTGTCTTAATATTCAGCCAGTTGGGTCAGTATGCTCGACAGCTTCTTGCAATGGATTTCCAATGGCAAAATATTATTGTAATATTTGCAACCTCTTTGATGATGACAGGAATGTCTACCACTGTCCATTTTGCAACTTATGCCGAGTAGGGAAGGGCCTTGGCATTGATTATTTTCACTGCATGTCGTGCAATTGTTGCATGGGGATGGCTTCGGTGAACCACAAGTGCCAGGAGAAATGTCTAGAAACCAACTGCCCTGTCTGCAATGAGTTCTTATTCACATCAAGTTCAAGAATCAAAGCTTTACCATGTGGCCATTGTATGCATTTAGCTTGCTTCAAGACTTGCACTCAGAGCAACAGTCAATACACTTGTCCAGTTTGCAGCAAATCTTCAGGAGCTACGATGGTAATAAAAGTAATAGGCTTATGTTTATATATATATTTTTTAAAAAAAATTCAATCTTTGAGGACTTATATACTTCATTTACACACCAAAAATGATAAAATGCGGTTAGTTACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0008270 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044464 GO:0046872 GO:0046914 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Hemerythrin zf-CHY zf-RING_2 zinc_ribbon_6

Protein Analysis

808

Amino Acids

91.84

Weight (kDa)

6.23

Isoelectric Point (pI)

50.96

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-CHY	PF05495	590 - 658	3.5e-18	CHY zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014789)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g05930 FvH4_7g05930 FvH4_7g05930
malus_domestica	MD07G1068200.v1.1
prunus_persica	Prupe.2G085600_v2.0.a1
pyrus_communis	pycom07g05230
rosa_chinensis	RchiOBHm_Chr1g0333131
rosa_laevigata	RLG00000029624
rosa_multiflora	Rmu_sc0007432.1_g000001
rosa_roxburghii	Rroxscaffold_4G00317770
rosa_rugosa	Rorug01G0107300 Rorug01G0107400
rosa_samantha	Rh1AG130600 Rh1BG099700 Rh1CG124400 Rh1DG135900
rosa_wichuraiana	Rw1G010240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2012
Acc36I	ACCTGC	1 cut(s)	862
AccB1I	GGYRCC	1 cut(s)	1181
AccB7I	CCANNNNNTGG	2 cut(s)	1310, 1864
AccI	GTMKAC	1 cut(s)	2016
AccII	CGCG	1 cut(s)	1621
AciI	CCGC	6 cut(s)	194, 1503, 1532, 1621, 1812, 2413
AclI	AACGTT	1 cut(s)	1736
AcoI	YGGCCR	2 cut(s)	1301, 2215
AcsI	RAATTY	5 cut(s)	279, 339, 446, 667, 2358
AcuI	CTGAAG	3 cut(s)	947, 1398, 2280
AfaI	GTAC	7 cut(s)	9, 528, 897, 1171, 1211, 1280, 1567
AfiI	CCNNNNNNNGG	4 cut(s)	514, 907, 1310, 1864
AflIII	ACRYGT	1 cut(s)	1275
AhlI	ACTAGT	1 cut(s)	1646
AjnI	CCWGG	2 cut(s)	44, 2130
Alw21I	GWGCWC	4 cut(s)	360, 1012, 1275, 1318
Alw26I	GTCTC	3 cut(s)	164, 1053, 1442
AlwNI	CAGNNNCTG	2 cut(s)	443, 1376
AoxI	GGCC	4 cut(s)	891, 1301, 2056, 2215
ApeKI	GCWGC	9 cut(s)	262, 440, 692, 721, 1661, 1767, 1802, 1809, 2286
ApoI	RAATTY	5 cut(s)	279, 339, 446, 667, 2358
ArsI	GACNNNNNNTTYG	2 cut(s)	1063, 1095
AseI	ATTAAT	1 cut(s)	231
Asp700I	GAANNNNTTC	2 cut(s)	312, 957
AspLEI	GCGC	2 cut(s)	419, 502
AspS9I	GGNCC	1 cut(s)	2056
AsuC2I	CCSGG	1 cut(s)	1283
AsuHPI	GGTGA	5 cut(s)	34, 792, 1580, 1722, 2128
AsuII	TTCGAA	2 cut(s)	541, 1234
BaeGI	GKGCMC	1 cut(s)	1186
BalI	TGGCCA	2 cut(s)	1303, 2217
BanI	GGYRCC	1 cut(s)	1181
BanII	GRGCYC	1 cut(s)	1012
BarI	GAAGNNNNNNTAC	2 cut(s)	510, 542
Bbv12I	GWGCWC	4 cut(s)	360, 1012, 1275, 1318
BbvCI	CCTCAGC	1 cut(s)	303
BbvI	GCAGC	9 cut(s)	249, 427, 704, 733, 1648, 1754, 1789, 1796, 2298
BccI	CCATC	6 cut(s)	43, 811, 1255, 1593, 2101, 2302
BceAI	ACGGC	2 cut(s)	878, 885
BciT130I	CCWGG	2 cut(s)	46, 2132
BciVI	GTATCC	2 cut(s)	141, 1050
BclI	TGATCA	3 cut(s)	475, 1096, 1597
BcnI	CCSGG	1 cut(s)	1283
BcoDI	GTCTC	3 cut(s)	164, 1053, 1442
BcuI	ACTAGT	1 cut(s)	1646
BfaI	CTAG	6 cut(s)	741, 917, 1548, 1647, 1799, 2144
BfmI	CTRYAG	3 cut(s)	324, 1368, 1680
BfuAI	ACCTGC	1 cut(s)	862
BfuI	GTATCC	2 cut(s)	141, 1050
BglII	AGATCT	1 cut(s)	1175
BlpI	GCTNAGC	1 cut(s)	1665
BmcAI	AGTACT	1 cut(s)	1211
Bme1390I	CCNGG	3 cut(s)	46, 1283, 2132
BmgT120I	GGNCC	1 cut(s)	2056
BmiI	GGNNCC	1 cut(s)	1183
BmrFI	CCNGG	3 cut(s)	46, 1283, 2132
BmrI	ACTGGG	1 cut(s)	1370
BmsI	GCATC	7 cut(s)	166, 428, 588, 589, 710, 1105, 1645
BmuI	ACTGGG	1 cut(s)	1370
BpmI	CTGGAG	2 cut(s)	240, 681
Bpu10I	CCTNAGC	1 cut(s)	303
Bpu1102I	GCTNAGC	1 cut(s)	1665
Bpu14I	TTCGAA	2 cut(s)	541, 1234
BpuEI	CTTGAG	3 cut(s)	571, 1166, 1313
BpuMI	CCSGG	1 cut(s)	1283
BsaI	GGTCTC	1 cut(s)	1442
BsaJI	CCNNGG	3 cut(s)	629, 1400, 2059
BsaWI	WCCGGW	1 cut(s)	1540
Bsc4I	CCNNNNNNNGG	4 cut(s)	514, 907, 1310, 1864
Bse1I	ACTGG	8 cut(s)	249, 720, 850, 1365, 1573, 1658, 1919, 2279
Bse3DI	GCAATG	3 cut(s)	1053, 1957, 2173
BseBI	CCWGG	2 cut(s)	46, 2132
BseDI	CCNNGG	3 cut(s)	629, 1400, 2059
BseGI	GGATG	4 cut(s)	54, 1432, 1880, 2112
BseLI	CCNNNNNNNGG	4 cut(s)	514, 907, 1310, 1864
BseMI	GCAATG	3 cut(s)	1053, 1957, 2173
BseMII	CTCAG	4 cut(s)	294, 1413, 1679, 2267
BseNI	ACTGG	8 cut(s)	249, 720, 850, 1365, 1573, 1658, 1919, 2279
BseRI	GAGGAG	1 cut(s)	1346
BseSI	GKGCMC	1 cut(s)	1186
BseXI	GCAGC	9 cut(s)	249, 427, 704, 733, 1648, 1754, 1789, 1796, 2298
BsgI	GTGCAG	3 cut(s)	740, 840, 1398
Bsh1236I	CGCG	1 cut(s)	1621
BshFI	GGCC	4 cut(s)	893, 1303, 2058, 2217
BshNI	GGYRCC	1 cut(s)	1181
BsiHKAI	GWGCWC	4 cut(s)	360, 1012, 1275, 1318
BsiSI	CCGG	3 cut(s)	1043, 1282, 1541
BsiWI	CGTACG	1 cut(s)	895
BslFI	GGGAC	2 cut(s)	1378, 1737
BslI	CCNNNNNNNGG	4 cut(s)	514, 907, 1310, 1864
BsmAI	GTCTC	3 cut(s)	164, 1053, 1442
BsmFI	GGGAC	2 cut(s)	1378, 1737
BsmI	GAATGC	1 cut(s)	1505
BsnI	GGCC	4 cut(s)	893, 1303, 2058, 2217
Bso31I	GGTCTC	1 cut(s)	1442
Bsp119I	TTCGAA	2 cut(s)	541, 1234
Bsp1286I	GDGCHC	5 cut(s)	360, 1012, 1186, 1275, 1318
Bsp143I	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
Bsp1720I	GCTNAGC	1 cut(s)	1665
Bsp19I	CCATGG	2 cut(s)	629, 1400
BspACI	CCGC	6 cut(s)	194, 1503, 1532, 1621, 1812, 2413
BspANI	GGCC	4 cut(s)	893, 1303, 2058, 2217
BspCNI	CTCAG	4 cut(s)	295, 1414, 1678, 2266
BspFNI	CGCG	1 cut(s)	1621
BspHI	TCATGA	1 cut(s)	1747
BspLI	GGNNCC	1 cut(s)	1183
BspMAI	CTGCAG	1 cut(s)	328
BspMI	ACCTGC	1 cut(s)	862
BspQI	GCTCTTC	1 cut(s)	1281
BspT104I	TTCGAA	2 cut(s)	541, 1234
BspT107I	GGYRCC	1 cut(s)	1181
BspTNI	GGTCTC	1 cut(s)	1442
BsrDI	GCAATG	3 cut(s)	1053, 1957, 2173
BsrI	ACTGG	8 cut(s)	249, 720, 850, 1365, 1573, 1658, 1919, 2279
BssECI	CCNNGG	3 cut(s)	629, 1400, 2059
BssMI	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
BssT1I	CCWWGG	3 cut(s)	629, 1400, 2059
Bst2UI	CCWGG	2 cut(s)	46, 2132
Bst6I	CTCTTC	2 cut(s)	192, 1281
BstAPI	GCANNNNNTGC	3 cut(s)	2040, 2088, 2097
BstBI	TTCGAA	2 cut(s)	541, 1234
BstC8I	GCNNGC	4 cut(s)	1221, 1772, 1807, 2236
BstDEI	CTNAG	7 cut(s)	303, 938, 1253, 1422, 1493, 1665, 2253
BstDSI	CCRYGG	2 cut(s)	629, 1400
BstF5I	GGATG	4 cut(s)	54, 1432, 1880, 2112
BstFNI	CGCG	1 cut(s)	1621
BstHHI	GCGC	2 cut(s)	419, 502
BstKTI	GATC	7 cut(s)	88, 478, 1099, 1178, 1350, 1600, 1747
BstMAI	GTCTC	3 cut(s)	164, 1053, 1442
BstMBI	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
BstMWI	GCNNNNNNNGC	9 cut(s)	625, 659, 698, 746, 2040, 2088, 2097, 2163, 2256
BstNI	CCWGG	2 cut(s)	46, 2132
BstNSI	RCATGY	4 cut(s)	289, 685, 1279, 2086
BstSCI	CCNGG	3 cut(s)	44, 1281, 2130
BstSFI	CTRYAG	3 cut(s)	324, 1368, 1680
BstSLI	GKGCMC	1 cut(s)	1186
BstUI	CGCG	1 cut(s)	1621
BstV1I	GCAGC	9 cut(s)	249, 427, 704, 733, 1648, 1754, 1789, 1796, 2298
BstX2I	RGATCY	1 cut(s)	1175
BstYI	RGATCY	1 cut(s)	1175
BsuI	GTATCC	2 cut(s)	141, 1050
BsuRI	GGCC	4 cut(s)	893, 1303, 2058, 2217
BtgI	CCRYGG	2 cut(s)	629, 1400
BtsCI	GGATG	4 cut(s)	54, 1432, 1880, 2112
BtsI	GCAGTG	2 cut(s)	333, 2077
BtsIMutI	CAGTG	9 cut(s)	333, 713, 843, 921, 1517, 1698, 1764, 2020, 2077
BveI	ACCTGC	1 cut(s)	862
Cac8I	GCNNGC	4 cut(s)	1221, 1772, 1807, 2236
CaiI	CAGNNNCTG	2 cut(s)	443, 1376
CciI	TCATGA	1 cut(s)	1747
CfoI	GCGC	2 cut(s)	419, 502
Cfr13I	GGNCC	1 cut(s)	2056
Csp6I	GTAC	7 cut(s)	8, 527, 896, 1170, 1210, 1279, 1566
CspCI	CAANNNNNGTGG	2 cut(s)	1674, 1709
CviQI	GTAC	7 cut(s)	8, 527, 896, 1170, 1210, 1279, 1566
DdeI	CTNAG	7 cut(s)	303, 938, 1253, 1422, 1493, 1665, 2253
DpnI	GATC	7 cut(s)	87, 477, 1098, 1177, 1349, 1599, 1746
DpnII	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
DraI	TTTAAA	4 cut(s)	411, 495, 880, 2352
DrdI	GACNNNNNNGTC	1 cut(s)	2012
DseDI	GACNNNNNNGTC	1 cut(s)	2012
EaeI	YGGCCR	2 cut(s)	1301, 2215
Eam1104I	CTCTTC	2 cut(s)	192, 1281
EarI	CTCTTC	2 cut(s)	192, 1281
Ecl136II	GAGCTC	1 cut(s)	1010
Eco130I	CCWWGG	3 cut(s)	629, 1400, 2059
Eco24I	GRGCYC	1 cut(s)	1012
Eco31I	GGTCTC	1 cut(s)	1442
Eco32I	GATATC	1 cut(s)	709
Eco53kI	GAGCTC	1 cut(s)	1010
Eco57I	CTGAAG	3 cut(s)	947, 1398, 2280
EcoICRI	GAGCTC	1 cut(s)	1010
EcoO109I	RGGNCCY	1 cut(s)	2056
EcoRII	CCWGG	2 cut(s)	44, 2130
EcoRV	GATATC	1 cut(s)	709
EcoT14I	CCWWGG	3 cut(s)	629, 1400, 2059
EcoT22I	ATGCAT	2 cut(s)	1120, 2229
EcoT38I	GRGCYC	1 cut(s)	1012
ErhI	CCWWGG	3 cut(s)	629, 1400, 2059
FaqI	GGGAC	2 cut(s)	1378, 1737
FauI	CCCGC	1 cut(s)	1496
FauNDI	CATATG	1 cut(s)	112
FbaI	TGATCA	3 cut(s)	475, 1096, 1597
FblI	GTMKAC	1 cut(s)	2016
FokI	GGATG	4 cut(s)	61, 1439, 1867, 2119
FriOI	GRGCYC	1 cut(s)	1012
FspBI	CTAG	6 cut(s)	741, 917, 1548, 1647, 1799, 2144
GlaI	GCGC	2 cut(s)	418, 501
GsuI	CTGGAG	2 cut(s)	240, 681
HaeIII	GGCC	4 cut(s)	893, 1303, 2058, 2217
HapII	CCGG	3 cut(s)	1043, 1282, 1541
HhaI	GCGC	2 cut(s)	419, 502
Hin6I	GCGC	2 cut(s)	417, 500
HinP1I	GCGC	2 cut(s)	417, 500
HincII	GTYRAC	2 cut(s)	648, 1524
HindII	GTYRAC	2 cut(s)	648, 1524
HindIII	AAGCTT	3 cut(s)	20, 1229, 2202
HinfI	GANTC	3 cut(s)	926, 1052, 2196
HpaII	CCGG	3 cut(s)	1043, 1282, 1541
HphI	GGTGA	5 cut(s)	34, 792, 1580, 1722, 2128
Hpy166II	GTNNAC	6 cut(s)	648, 1524, 1712, 1824, 2017, 2119
Hpy188I	TCNGA	4 cut(s)	585, 706, 931, 2256
Hpy8I	GTNNAC	6 cut(s)	648, 1524, 1712, 1824, 2017, 2119
HpyAV	CCTTC	4 cut(s)	47, 441, 1462, 2047
HpyCH4IV	ACGT	1 cut(s)	1736
HpyF10VI	GCNNNNNNNGC	9 cut(s)	625, 659, 698, 746, 2040, 2088, 2097, 2163, 2256
HpyF3I	CTNAG	7 cut(s)	303, 938, 1253, 1422, 1493, 1665, 2253
HpySE526I	ACGT	1 cut(s)	1736
HspAI	GCGC	2 cut(s)	417, 500
Ksp22I	TGATCA	3 cut(s)	475, 1096, 1597
Kzo9I	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
LguI	GCTCTTC	1 cut(s)	1281
LmnI	GCTCC	5 cut(s)	470, 700, 1313, 1333, 2300
Lsp1109I	GCAGC	9 cut(s)	249, 427, 704, 733, 1648, 1754, 1789, 1796, 2298
LweI	GCATC	7 cut(s)	166, 428, 588, 589, 710, 1105, 1645
MaeI	CTAG	6 cut(s)	741, 917, 1548, 1647, 1799, 2144
MaeII	ACGT	1 cut(s)	1736
MaeIII	GTNAC	9 cut(s)	902, 941, 1079, 1395, 1405, 1462, 1649, 1728, 2419
MalI	GATC	7 cut(s)	87, 477, 1098, 1177, 1349, 1599, 1746
MboI	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
MboII	GAAGA	9 cut(s)	209, 214, 217, 953, 1124, 1133, 1298, 1589, 2286
MfeI	CAATTG	2 cut(s)	64, 2092
MflI	RGATCY	1 cut(s)	1175
MhlI	GDGCHC	5 cut(s)	360, 1012, 1186, 1275, 1318
MlsI	TGGCCA	2 cut(s)	1303, 2217
MluNI	TGGCCA	2 cut(s)	1303, 2217
Mox20I	TGGCCA	2 cut(s)	1303, 2217
Mph1103I	ATGCAT	2 cut(s)	1120, 2229
MroXI	GAANNNNTTC	2 cut(s)	312, 957
MscI	TGGCCA	2 cut(s)	1303, 2217
MslI	CAYNNNNRTG	5 cut(s)	47, 363, 507, 1017, 2105
Msp20I	TGGCCA	2 cut(s)	1303, 2217
MspA1I	CMGCKG	2 cut(s)	83, 440
MspI	CCGG	3 cut(s)	1043, 1282, 1541
MspR9I	CCNGG	3 cut(s)	46, 1283, 2132
MunI	CAATTG	2 cut(s)	64, 2092
Mva1269I	GAATGC	1 cut(s)	1505
MvaI	CCWGG	2 cut(s)	46, 2132
MvnI	CGCG	1 cut(s)	1621
MwoI	GCNNNNNNNGC	9 cut(s)	625, 659, 698, 746, 2040, 2088, 2097, 2163, 2256
NciI	CCSGG	1 cut(s)	1283
NcoI	CCATGG	2 cut(s)	629, 1400
NdeI	CATATG	1 cut(s)	112
NdeII	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
NlaIV	GGNNCC	1 cut(s)	1183
NmeAIII	GCCGAG	2 cut(s)	40, 2069
NmuCI	GTSAC	3 cut(s)	902, 1079, 1728
NsiI	ATGCAT	2 cut(s)	1120, 2229
NspI	RCATGY	4 cut(s)	289, 685, 1279, 2086
NspV	TTCGAA	2 cut(s)	541, 1234
PagI	TCATGA	1 cut(s)	1747
PciI	ACATGT	1 cut(s)	1275
PciSI	GCTCTTC	1 cut(s)	1281
PctI	GAATGC	1 cut(s)	1505
PdmI	GAANNNNTTC	2 cut(s)	312, 957
PfeI	GAWTC	3 cut(s)	926, 1052, 2196
Pfl23II	CGTACG	1 cut(s)	895
PflMI	CCANNNNNTGG	2 cut(s)	1310, 1864
PscI	ACATGT	1 cut(s)	1275
PshBI	ATTAAT	1 cut(s)	231
Psp124BI	GAGCTC	1 cut(s)	1012
Psp1406I	AACGTT	1 cut(s)	1736
Psp6I	CCWGG	2 cut(s)	44, 2130
PspGI	CCWGG	2 cut(s)	44, 2130
PspLI	CGTACG	1 cut(s)	895
PspN4I	GGNNCC	1 cut(s)	1183
PspPI	GGNCC	1 cut(s)	2056
PsrI	GAACNNNNNNTAC	2 cut(s)	867, 899
PstI	CTGCAG	1 cut(s)	328
PstNI	CAGNNNCTG	2 cut(s)	443, 1376
PsuI	RGATCY	1 cut(s)	1175
PvuII	CAGCTG	2 cut(s)	83, 440
RsaI	GTAC	7 cut(s)	9, 528, 897, 1171, 1211, 1280, 1567
RsaNI	GTAC	7 cut(s)	8, 527, 896, 1170, 1210, 1279, 1566
RseI	CAYNNNNRTG	5 cut(s)	47, 363, 507, 1017, 2105
SacI	GAGCTC	1 cut(s)	1012
SapI	GCTCTTC	1 cut(s)	1281
Sau3AI	GATC	7 cut(s)	85, 475, 1096, 1175, 1347, 1597, 1744
Sau96I	GGNCC	1 cut(s)	2056
ScaI	AGTACT	1 cut(s)	1211
ScrFI	CCNGG	3 cut(s)	46, 1283, 2132
SduI	GDGCHC	5 cut(s)	360, 1012, 1186, 1275, 1318
SfaNI	GCATC	7 cut(s)	166, 428, 588, 589, 710, 1105, 1645
SfcI	CTRYAG	3 cut(s)	324, 1368, 1680
SfuI	TTCGAA	2 cut(s)	541, 1234
SmiMI	CAYNNNNRTG	5 cut(s)	47, 363, 507, 1017, 2105
SmlI	CTYRAG	3 cut(s)	550, 1145, 1292
SmoI	CTYRAG	3 cut(s)	550, 1145, 1292
SpeI	ACTAGT	1 cut(s)	1646
SsiI	CCGC	6 cut(s)	194, 1503, 1532, 1621, 1812, 2413
SspI	AATATT	5 cut(s)	957, 1129, 1912, 1973, 1984
SspMI	CTAG	6 cut(s)	741, 917, 1548, 1647, 1799, 2144
SstI	GAGCTC	1 cut(s)	1012
StyD4I	CCNGG	3 cut(s)	44, 1281, 2130
StyI	CCWWGG	3 cut(s)	629, 1400, 2059
TaiI	ACGT	1 cut(s)	1739
TaqI	TCGA	6 cut(s)	88, 402, 541, 1234, 1741, 1937
TatI	WGTACW	1 cut(s)	1209
TauI	GCSGC	1 cut(s)	1815
TfiI	GAWTC	3 cut(s)	926, 1052, 2196
TscAI	CASTG	9 cut(s)	333, 720, 850, 928, 1524, 1698, 1764, 2027, 2084
TseFI	GTSAC	3 cut(s)	902, 1079, 1728
TseI	GCWGC	9 cut(s)	262, 440, 692, 721, 1661, 1767, 1802, 1809, 2286
Tsp45I	GTSAC	3 cut(s)	902, 1079, 1728
TspGWI	ACGGA	2 cut(s)	1447, 1460
TspRI	CASTG	9 cut(s)	333, 720, 850, 928, 1524, 1698, 1764, 2027, 2084
Van91I	CCANNNNNTGG	2 cut(s)	1310, 1864
VspI	ATTAAT	1 cut(s)	231
XapI	RAATTY	5 cut(s)	279, 339, 446, 667, 2358
XbaI	TCTAGA	2 cut(s)	916, 2143
XceI	RCATGY	4 cut(s)	289, 685, 1279, 2086
XmiI	GTMKAC	1 cut(s)	2016
XmnI	GAANNNNTTC	2 cut(s)	312, 957
XspI	CTAG	6 cut(s)	741, 917, 1548, 1647, 1799, 2144
ZrmI	AGTACT	1 cut(s)	1211
Zsp2I	ATGCAT	2 cut(s)	1120, 2229

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.