MD07G1082500.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0013614
Physical Location & Seq
Reverse (-)
8088135 .. 8090557
2423 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1082500.v1.1.491

Sequence Viewer

Length: 786 bp
ATGAACAAAGAAGAAGTGTCTCGCATGCGTGAAGGCACAGATCCTTCATCTGAGAATGCGGAGGGGCACCCTGAAGGTTTGCAGTTGGAGATAATGAACCAGAACCATGAAAATCCAGAGCATCTGATGGAGAAAACAACCAAAACTTGTTCAAGGAAGAAAACAAAGTACAATTGTACTGTTGTTAGGCGATCCTTGCGTTTCCAAAATGGTATTCTGCCTGCTGGAAACGAGAATACAGAACTTGTGCTTGAAGATACAACCGCTGGCGAAAGTGGAAGTGCAGAAGAACAACCTGCCCATAAAATGCACGGGTCAACCTCAGGCGACAAAAGCTTGGAAGAAAAAGTTGACTATATTATGCAACTTTTGGAAGCGAGGAACTCAAAGGCACGGGGTAAACACTCATCCTTCTGTGAAAGCCCAGAGTCGATGTACAAAAGCTCATATGCAGATTCCCAAAAGATTGAGGCTTTAGCAAACGAAAACCATAAGCTGTCAATGAAGTTGGAAATTGCTCTTGCCAAACTTGAGGCATACGAGAGCAGGACTCATGCATTTTCTGAAATGGCGGAGAAACTGAAGGATGTGATTGTAGTTTCAAATTTGTCAAAAGTTACAGAAACTGCAGTTAATCTGTCATCTCAGACGGCACATGGAGATCTTGTGCCACAGGAGGAGTTTCTTTCGCATTTCCTTAACCGGAAAAGGCCGAATGCTCAGTCACCCAAAGCTGCAGCAAAGAGAAAGAAACAAGAGAAGAAAGCAAATCGAAGGTACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

262

Amino Acids

29.35

Weight (kDa)

7.71

Isoelectric Point (pI)

57.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 304
AccB1I GGYRCC 1 cut(s) 66
AciI CCGC 3 cut(s) 59, 264, 572
AclWI GGATC 2 cut(s) 35, 186
AcsI RAATTY 1 cut(s) 604
AcuI CTGAAG 2 cut(s) 93, 602
AfaI GTAC 4 cut(s) 170, 178, 437, 779
AgsI TTSAA 3 cut(s) 153, 254, 603
AjuI GAANNNNNNNTTGG 2 cut(s) 198, 230
AluBI AGCT 4 cut(s) 336, 444, 496, 734
AluI AGCT 4 cut(s) 336, 444, 496, 734
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 2 cut(s) 35, 186
AlwNI CAGNNNCTG 1 cut(s) 626
AoxI GGCC 1 cut(s) 710
ApeKI GCWGC 2 cut(s) 734, 737
ApoI RAATTY 1 cut(s) 604
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AsuHPI GGTGA 1 cut(s) 717
AxyI CCTNAGG 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 69
BanI GGYRCC 1 cut(s) 66
BbvI GCAGC 2 cut(s) 721, 749
BccI CCATC 1 cut(s) 121
BceAI ACGGC 1 cut(s) 666
BcoDI GTCTC 1 cut(s) 24
BfmI CTRYAG 2 cut(s) 627, 735
BfuAI ACCTGC 1 cut(s) 304
BglII AGATCT 1 cut(s) 661
BisI GCNGC 2 cut(s) 735, 738
BlsI GCNGC 2 cut(s) 736, 739
BmiI GGNNCC 1 cut(s) 68
BmsI GCATC 1 cut(s) 130
BplI GAGNNNNNCTC 2 cut(s) 535, 567
BpuEI CTTGAG 1 cut(s) 551
BsaWI WCCGGW 1 cut(s) 702
BsaXI ACNNNNNCTCC 2 cut(s) 651, 681
Bse21I CCTNAGG 1 cut(s) 322
BseGI GGATG 2 cut(s) 407, 592
BseMII CTCAG 4 cut(s) 42, 336, 659, 734
BseRI GAGGAG 1 cut(s) 692
BseSI GKGCMC 1 cut(s) 69
BseXI GCAGC 2 cut(s) 721, 749
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 1 cut(s) 712
BshNI GGYRCC 1 cut(s) 66
BsiSI CCGG 1 cut(s) 703
BsmAI GTCTC 1 cut(s) 24
BsmI GAATGC 2 cut(s) 61, 721
BsnI GGCC 1 cut(s) 712
Bsp1286I GDGCHC 1 cut(s) 69
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 3 cut(s) 40, 191, 661
BspACI CCGC 3 cut(s) 59, 264, 572
BspANI GGCC 1 cut(s) 712
BspCNI CTCAG 4 cut(s) 43, 335, 658, 733
BspLI GGNNCC 1 cut(s) 68
BspMAI CTGCAG 2 cut(s) 631, 739
BspMI ACCTGC 1 cut(s) 304
BspPI GGATC 2 cut(s) 35, 186
BspT107I GGYRCC 1 cut(s) 66
BsrGI TGTACA 1 cut(s) 435
BssMI GATC 3 cut(s) 40, 191, 661
Bst4CI ACNGT 2 cut(s) 181, 782
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 3 cut(s) 26, 222, 268
BstDEI CTNAG 4 cut(s) 51, 322, 645, 720
BstF5I GGATG 2 cut(s) 407, 592
BstKTI GATC 3 cut(s) 43, 194, 664
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 3 cut(s) 40, 191, 661
BstMWI GCNNNNNNNGC 2 cut(s) 196, 333
BstNSI RCATGY 1 cut(s) 28
BstSFI CTRYAG 2 cut(s) 627, 735
BstSLI GKGCMC 1 cut(s) 69
BstV1I GCAGC 2 cut(s) 721, 749
BstX2I RGATCY 2 cut(s) 40, 661
BstYI RGATCY 2 cut(s) 40, 661
Bsu36I CCTNAGG 1 cut(s) 322
BsuRI GGCC 1 cut(s) 712
BtsCI GGATG 2 cut(s) 407, 592
BveI ACCTGC 1 cut(s) 304
Cac8I GCNNGC 3 cut(s) 26, 222, 268
CaiI CAGNNNCTG 1 cut(s) 626
Csp6I GTAC 4 cut(s) 169, 177, 436, 778
CviAII CATG 4 cut(s) 25, 107, 554, 656
CviJI RGCY 7 cut(s) 336, 423, 444, 473, 496, 712, 734
CviKI_1 RGCY 7 cut(s) 336, 423, 444, 473, 496, 712, 734
CviQI GTAC 4 cut(s) 169, 177, 436, 778
DdeI CTNAG 4 cut(s) 51, 322, 645, 720
DpnI GATC 3 cut(s) 42, 193, 663
DpnII GATC 3 cut(s) 40, 191, 661
EciI GGCGGA 1 cut(s) 587
Eco57I CTGAAG 2 cut(s) 93, 602
Eco81I CCTNAGG 1 cut(s) 322
EcoT22I ATGCAT 1 cut(s) 559
FaeI CATG 4 cut(s) 28, 110, 557, 659
FatI CATG 4 cut(s) 24, 106, 553, 655
FauNDI CATATG 1 cut(s) 448
Fnu4HI GCNGC 2 cut(s) 735, 738
FokI GGATG 2 cut(s) 394, 599
Fsp4HI GCNGC 2 cut(s) 735, 738
GluI GCNGC 2 cut(s) 735, 738
HaeIII GGCC 1 cut(s) 712
HapII CCGG 1 cut(s) 703
Hin1II CATG 4 cut(s) 28, 110, 557, 659
HincII GTYRAC 2 cut(s) 318, 352
HindII GTYRAC 2 cut(s) 318, 352
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 3 cut(s) 428, 455, 550
HpaII CCGG 1 cut(s) 703
HphI GGTGA 1 cut(s) 717
Hpy166II GTNNAC 3 cut(s) 318, 352, 401
Hpy188I TCNGA 4 cut(s) 52, 126, 565, 648
Hpy188III TCNNGA 1 cut(s) 116
Hpy8I GTNNAC 3 cut(s) 318, 352, 401
HpyAV CCTTC 6 cut(s) 26, 54, 68, 421, 577, 768
HpyCH4III ACNGT 2 cut(s) 181, 782
HpyCH4V TGCA 8 cut(s) 82, 284, 310, 364, 452, 557, 629, 737
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 333
HpyF3I CTNAG 4 cut(s) 51, 322, 645, 720
Hsp92II CATG 4 cut(s) 28, 110, 557, 659
Kzo9I GATC 3 cut(s) 40, 191, 661
Lsp1109I GCAGC 2 cut(s) 721, 749
LweI GCATC 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 616, 723
MalI GATC 3 cut(s) 42, 193, 663
MboI GATC 3 cut(s) 40, 191, 661
MboII GAAGA 6 cut(s) 23, 169, 266, 299, 353, 772
MfeI CAATTG 1 cut(s) 172
MflI RGATCY 2 cut(s) 40, 661
MhlI GDGCHC 1 cut(s) 69
MluCI AATT 3 cut(s) 172, 513, 604
MlyI GAGTC 2 cut(s) 437, 544
MmeI TCCRAC 2 cut(s) 66, 489
MnlI CCTC 6 cut(s) 55, 331, 372, 463, 526, 670
Mph1103I ATGCAT 1 cut(s) 559
MseI TTAA 2 cut(s) 633, 699
MspA1I CMGCKG 1 cut(s) 266
MspI CCGG 1 cut(s) 703
MunI CAATTG 1 cut(s) 172
Mva1269I GAATGC 2 cut(s) 61, 721
MwoI GCNNNNNNNGC 2 cut(s) 196, 333
NdeI CATATG 1 cut(s) 448
NdeII GATC 3 cut(s) 40, 191, 661
NlaIII CATG 4 cut(s) 28, 110, 557, 659
NlaIV GGNNCC 1 cut(s) 68
NmuCI GTSAC 1 cut(s) 723
NsiI ATGCAT 1 cut(s) 559
NspI RCATGY 1 cut(s) 28
PaeI GCATGC 1 cut(s) 28
PctI GAATGC 2 cut(s) 61, 721
PfeI GAWTC 1 cut(s) 455
PkrI GCNGC 2 cut(s) 736, 739
PleI GAGTC 2 cut(s) 436, 544
PpsI GAGTC 2 cut(s) 436, 544
PspN4I GGNNCC 1 cut(s) 68
PstI CTGCAG 2 cut(s) 631, 739
PstNI CAGNNNCTG 1 cut(s) 626
PsuI RGATCY 2 cut(s) 40, 661
RsaI GTAC 4 cut(s) 170, 178, 437, 779
RsaNI GTAC 4 cut(s) 169, 177, 436, 778
SaqAI TTAA 2 cut(s) 633, 699
SatI GCNGC 2 cut(s) 735, 738
Sau3AI GATC 3 cut(s) 40, 191, 661
SchI GAGTC 2 cut(s) 437, 544
SduI GDGCHC 1 cut(s) 69
SetI ASST 8 cut(s) 79, 298, 323, 338, 446, 498, 736, 779
SfaNI GCATC 1 cut(s) 130
SfcI CTRYAG 2 cut(s) 627, 735
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SphI GCATGC 1 cut(s) 28
Sse9I AATT 3 cut(s) 172, 513, 604
SsiI CCGC 3 cut(s) 59, 264, 572
TaaI ACNGT 2 cut(s) 181, 782
TaqI TCGA 2 cut(s) 431, 772
TasI AATT 3 cut(s) 172, 513, 604
TatI WGTACW 3 cut(s) 168, 176, 435
TfiI GAWTC 1 cut(s) 455
Tru1I TTAA 2 cut(s) 633, 699
Tru9I TTAA 2 cut(s) 633, 699
TseFI GTSAC 1 cut(s) 723
TseI GCWGC 2 cut(s) 734, 737
Tsp45I GTSAC 1 cut(s) 723
TspDTI ATGAA 5 cut(s) 17, 36, 110, 123, 518
XapI RAATTY 1 cut(s) 604
XceI RCATGY 1 cut(s) 28
Zsp2I ATGCAT 1 cut(s) 559
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.