Rroxscaffold_7G00176410

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0013614
Physical Location & Seq
Forward (+)
15889619 .. 15891763
2145 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00176410.1

Sequence Viewer

Length: 654 bp
ATGGCTCAAACGCCTAGGTCCACTAATCTGAAAACTGAGAGAGTTCAGTCACAGCCTGAAAGACCAAATCTTCCTACGGAAGAAATGGCACTAGAGACTGCACCTCCACAGAAGAAAAGCAAGGCAAGGAAGAAACGGCCAAGGTACTACACAGCAGTTAGGCGATCTTCACGCATCCGAAACACTGTGACTCCCTCTCAAAACCGGAGCCTACAGCCAGTGCAGATTACAATCAGTGAAAGCGAGAGTGAAAGCGACAGAGAAGAGGAGCCTCCTTGTCTGGAGGAGCCGGCCGTCCGTGGTGAGAAAACATTGGATGAAAAAGTGGACTATGCTGTGCAGTTATTGGAGACTATGAGCTCACAGGGTAACAATAGGATCTCTGACACTTCTGAACTCAGATACAGAAGTTTGTACTTTGAGTCCCAGAAGCAGAATGAGAATTTGAAAAAGGAGAACCGCGAGCTTTCTTTGAAGTTGCAAGTTGCTCTTGCCAAAATTGAAGGATTTGAGAAAGGGAGTAATGCATCTGTGGAATGGATGGCAAAATTTAAAGATGTGTTGTTGGTTACAAGCTTGGCAAAAGCTTCTGAAATGGTTGCTTCTCAAGATCCTAAAGCGGCTTCTTCTTCAAAGAGAAAGAGACATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

217

Amino Acids

24.71

Weight (kDa)

9.52

Isoelectric Point (pI)

69.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 462
AciI CCGC 2 cut(s) 460, 620
AclWI GGATC 2 cut(s) 386, 605
AcoI YGGCCR 2 cut(s) 137, 291
AcsI RAATTY 2 cut(s) 442, 548
AfaI GTAC 2 cut(s) 146, 416
AgsI TTSAA 4 cut(s) 448, 475, 503, 633
AluBI AGCT 4 cut(s) 360, 466, 576, 587
AluI AGCT 4 cut(s) 360, 466, 576, 587
Alw21I GWGCWC 1 cut(s) 362
Alw26I GTCTC 3 cut(s) 89, 344, 637
AlwI GGATC 2 cut(s) 386, 605
AoxI GGCC 2 cut(s) 137, 291
ApoI RAATTY 2 cut(s) 442, 548
AspA2I CCTAGG 1 cut(s) 14
AspS9I GGNCC 1 cut(s) 18
AsuHPI GGTGA 1 cut(s) 314
AvaII GGWCC 1 cut(s) 18
AvrII CCTAGG 1 cut(s) 14
BaeI ACNNNNGTAYC 2 cut(s) 394, 427
BanII GRGCYC 1 cut(s) 362
Bbv12I GWGCWC 1 cut(s) 362
BccI CCATC 1 cut(s) 535
BceAI ACGGC 2 cut(s) 152, 278
BcoDI GTCTC 3 cut(s) 89, 344, 637
BfaI CTAG 2 cut(s) 15, 92
BfmI CTRYAG 1 cut(s) 212
BisI GCNGC 1 cut(s) 621
BlnI CCTAGG 1 cut(s) 14
BlsI GCNGC 1 cut(s) 622
Bme18I GGWCC 1 cut(s) 18
BmgT120I GGNCC 1 cut(s) 18
BmiI GGNNCC 3 cut(s) 209, 270, 288
BmsI GCATC 2 cut(s) 183, 536
BpmI CTGGAG 1 cut(s) 302
BpuEI CTTGAG 1 cut(s) 591
BsaBI GATNNNNATC 1 cut(s) 230
BsaJI CCNNGG 3 cut(s) 14, 140, 298
BsaWI WCCGGW 1 cut(s) 204
BsaXI ACNNNNNCTCC 4 cut(s) 88, 118, 175, 205
Bse118I RCCGGY 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 218
Bse8I GATNNNNATC 1 cut(s) 230
BseDI CCNNGG 3 cut(s) 14, 140, 298
BseGI GGATG 4 cut(s) 174, 322, 546, 646
BseJI GATNNNNATC 1 cut(s) 230
BseMII CTCAG 2 cut(s) 27, 412
BseNI ACTGG 1 cut(s) 218
BseRI GAGGAG 2 cut(s) 281, 299
BseX3I CGGCCG 1 cut(s) 291
BsgI GTGCAG 3 cut(s) 84, 242, 359
Bsh1236I CGCG 1 cut(s) 462
Bsh1285I CGRYCG 1 cut(s) 294
BshFI GGCC 2 cut(s) 139, 293
BsiEI CGRYCG 1 cut(s) 294
BsiHKAI GWGCWC 1 cut(s) 362
BsiSI CCGG 2 cut(s) 205, 290
BslFI GGGAC 1 cut(s) 409
BsmAI GTCTC 3 cut(s) 89, 344, 637
BsmFI GGGAC 1 cut(s) 409
BsnI GGCC 2 cut(s) 139, 293
Bsp1286I GDGCHC 1 cut(s) 362
Bsp143I GATC 3 cut(s) 164, 378, 610
BspACI CCGC 2 cut(s) 460, 620
BspANI GGCC 2 cut(s) 139, 293
BspCNI CTCAG 2 cut(s) 28, 411
BspFNI CGCG 1 cut(s) 462
BspLI GGNNCC 3 cut(s) 209, 270, 288
BspPI GGATC 2 cut(s) 386, 605
BsrFI RCCGGY 1 cut(s) 289
BsrI ACTGG 1 cut(s) 218
BssAI RCCGGY 1 cut(s) 289
BssECI CCNNGG 3 cut(s) 14, 140, 298
BssMI GATC 3 cut(s) 164, 378, 610
BssT1I CCWWGG 2 cut(s) 14, 140
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 291, 464
BstDEI CTNAG 2 cut(s) 36, 398
BstDSI CCRYGG 1 cut(s) 298
BstF5I GGATG 4 cut(s) 174, 322, 546, 646
BstFNI CGCG 1 cut(s) 462
BstKTI GATC 3 cut(s) 167, 381, 613
BstMAI GTCTC 3 cut(s) 89, 344, 637
BstMBI GATC 3 cut(s) 164, 378, 610
BstMCI CGRYCG 1 cut(s) 294
BstSFI CTRYAG 1 cut(s) 212
BstUI CGCG 1 cut(s) 462
BstX2I RGATCY 2 cut(s) 378, 610
BstYI RGATCY 2 cut(s) 378, 610
BstZI CGGCCG 1 cut(s) 291
BsuRI GGCC 2 cut(s) 139, 293
BtgI CCRYGG 1 cut(s) 298
BtsCI GGATG 4 cut(s) 174, 322, 546, 646
BtsIMutI CAGTG 3 cut(s) 183, 225, 241
Cac8I GCNNGC 2 cut(s) 291, 464
Cfr10I RCCGGY 1 cut(s) 289
Cfr13I GGNCC 1 cut(s) 18
Csp6I GTAC 2 cut(s) 145, 415
CviQI GTAC 2 cut(s) 145, 415
DdeI CTNAG 2 cut(s) 36, 398
DpnI GATC 3 cut(s) 166, 380, 612
DpnII GATC 3 cut(s) 164, 378, 610
DraI TTTAAA 1 cut(s) 553
EaeI YGGCCR 2 cut(s) 137, 291
EagI CGGCCG 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 258
EarI CTCTTC 1 cut(s) 258
Ecl136II GAGCTC 1 cut(s) 360
EclXI CGGCCG 1 cut(s) 291
Eco130I CCWWGG 2 cut(s) 14, 140
Eco24I GRGCYC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 18
Eco52I CGGCCG 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 360
EcoICRI GAGCTC 1 cut(s) 360
EcoT14I CCWWGG 2 cut(s) 14, 140
EcoT22I ATGCAT 1 cut(s) 529
EcoT38I GRGCYC 1 cut(s) 362
ErhI CCWWGG 2 cut(s) 14, 140
FaiI YATR 2 cut(s) 333, 356
FalI AAGNNNNNCTT 2 cut(s) 474, 506
FaqI GGGAC 1 cut(s) 409
Fnu4HI GCNGC 1 cut(s) 621
FokI GGATG 4 cut(s) 161, 329, 553, 633
FriOI GRGCYC 1 cut(s) 362
Fsp4HI GCNGC 1 cut(s) 621
FspBI CTAG 2 cut(s) 15, 92
GluI GCNGC 1 cut(s) 621
GsuI CTGGAG 1 cut(s) 302
HaeIII GGCC 2 cut(s) 139, 293
HapII CCGG 2 cut(s) 205, 290
HindIII AAGCTT 2 cut(s) 574, 585
HinfI GANTC 2 cut(s) 190, 422
HpaII CCGG 2 cut(s) 205, 290
HphI GGTGA 1 cut(s) 314
Hpy166II GTNNAC 2 cut(s) 21, 328
Hpy188I TCNGA 6 cut(s) 30, 179, 385, 394, 401, 592
Hpy188III TCNNGA 2 cut(s) 281, 608
Hpy8I GTNNAC 2 cut(s) 21, 328
HpyAV CCTTC 1 cut(s) 497
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 5 cut(s) 101, 223, 340, 481, 527
HpyF3I CTNAG 2 cut(s) 36, 398
KroI GCCGGC 1 cut(s) 289
KroNI GCCGGC 1 cut(s) 291
Kzo9I GATC 3 cut(s) 164, 378, 610
LmnI GCTCC 3 cut(s) 207, 268, 286
LpnPI CCDG 7 cut(s) 69, 218, 231, 266, 303, 350, 440
LweI GCATC 2 cut(s) 183, 536
MaeI CTAG 2 cut(s) 15, 92
MaeIII GTNAC 4 cut(s) 48, 187, 368, 568
MalI GATC 3 cut(s) 166, 380, 612
MboI GATC 3 cut(s) 164, 378, 610
MboII GAAGA 8 cut(s) 62, 92, 124, 142, 159, 275, 618, 621
MflI RGATCY 2 cut(s) 378, 610
MhlI GDGCHC 1 cut(s) 362
MluCI AATT 3 cut(s) 442, 498, 548
MlyI GAGTC 2 cut(s) 184, 431
MnlI CCTC 5 cut(s) 114, 205, 259, 277, 282
Mph1103I ATGCAT 1 cut(s) 529
MroNI GCCGGC 1 cut(s) 289
MseI TTAA 1 cut(s) 552
MspI CCGG 2 cut(s) 205, 290
MvnI CGCG 1 cut(s) 462
NaeI GCCGGC 1 cut(s) 291
NdeII GATC 3 cut(s) 164, 378, 610
NgoMIV GCCGGC 1 cut(s) 289
NlaIV GGNNCC 3 cut(s) 209, 270, 288
NmuCI GTSAC 2 cut(s) 48, 187
NsiI ATGCAT 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 291
PkrI GCNGC 1 cut(s) 622
PleI GAGTC 2 cut(s) 184, 430
PpsI GAGTC 2 cut(s) 184, 430
Psp124BI GAGCTC 1 cut(s) 362
PspN4I GGNNCC 3 cut(s) 209, 270, 288
PspPI GGNCC 1 cut(s) 18
PsuI RGATCY 2 cut(s) 378, 610
RsaI GTAC 2 cut(s) 146, 416
RsaNI GTAC 2 cut(s) 145, 415
SacI GAGCTC 1 cut(s) 362
SaqAI TTAA 1 cut(s) 552
SatI GCNGC 1 cut(s) 621
Sau3AI GATC 3 cut(s) 164, 378, 610
Sau96I GGNCC 1 cut(s) 18
SchI GAGTC 2 cut(s) 184, 431
SduI GDGCHC 1 cut(s) 362
SetI ASST 7 cut(s) 20, 106, 146, 362, 468, 578, 589
SfaNI GCATC 2 cut(s) 183, 536
SfcI CTRYAG 1 cut(s) 212
SinI GGWCC 1 cut(s) 18
SmlI CTYRAG 1 cut(s) 606
SmoI CTYRAG 1 cut(s) 606
Sse9I AATT 3 cut(s) 442, 498, 548
SsiI CCGC 2 cut(s) 460, 620
SspMI CTAG 2 cut(s) 15, 92
SstI GAGCTC 1 cut(s) 362
StyI CCWWGG 2 cut(s) 14, 140
TaaI ACNGT 1 cut(s) 187
TasI AATT 3 cut(s) 442, 498, 548
TatI WGTACW 1 cut(s) 414
TauI GCSGC 1 cut(s) 623
Tru1I TTAA 1 cut(s) 552
Tru9I TTAA 1 cut(s) 552
TscAI CASTG 3 cut(s) 190, 225, 241
TseFI GTSAC 2 cut(s) 48, 187
Tsp45I GTSAC 2 cut(s) 48, 187
TspDTI ATGAA 1 cut(s) 333
TspGWI ACGGA 2 cut(s) 92, 287
TspRI CASTG 3 cut(s) 190, 225, 241
VpaK11BI GGWCC 1 cut(s) 18
XapI RAATTY 2 cut(s) 442, 548
XmaJI CCTAGG 1 cut(s) 14
XspI CTAG 2 cut(s) 15, 92
Zsp2I ATGCAT 1 cut(s) 529
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.