MD07G1087100.v1.1

Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0004077
Physical Location & Seq
Reverse (-)
9084773 .. 9089910
5138 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1087100.v1.1.491

Sequence Viewer

Length: 639 bp
ATGGGTTCAGTTGTTGATGACGGAAAGAAGGAGGCTCTGCAGGAGGCTCATAGAAGCTTAGCTGATCTGAACCCTACAGTTGTTTTTGTATTGGGTGGCCCAGGCAGTGGAAAGGGTACTCAATGTGCAAACATTGTTGAGCACTTTGGGTTTACCCATCTTAGTGCTGGCGATCTTCTTAGAGCAGAAATCAAATCTGGCTCTGAAAATGGGACCATGATTTCGGATATGATTAAAGAAGGAAAGATTGTCCCTTCCGAAGTAACAATTAAACTTCTTGAACGAGCAATGCTGGAAAATGGTAATGACAAGTTTCTCATTGATGGGTTTCCCCGTAACGAGGAAAATCGTGCTGCATTTGAGGATGTGACTAAAATCGGGCCTTCATTTGTCTTGTATTTTGACTGTTCTGAAGAAGAGATGGAGAGGCGGCTTTTAAGTAGGAACCAGGGAAGAGAGGATGATAACATAGAAACAATAAGGAAGCGGTTTAAGGTTTTCCAAGAATCGAGTCTCCCTGTGATAGAGTACTACAACTCGAAGGGGAAAGTTCGGAAGATTGATGCTGGAAGGCCTATTGAAGAGGTTTATGAGTCAGTAAAAGCTATTTTTGCACCGAAAGATGAGAAGGCCGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00240 ko00983 ko01100 map00240 map00983 map01100
Pfam Domains
Protein Families
ADK

Protein Analysis

213

Amino Acids

23.66

Weight (kDa)

5.15

Isoelectric Point (pI)

40.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_33 PF13671 27 - 164 4.2e-07 AAA domain
AAA_18 PF13238 28 - 154 6.7e-09 AAA domain
ADK PF00406 30 - 182 2.3e-48 Adenylate kinase
AAA_17 PF13207 31 - 161 4.6e-38 AAA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 107
AciI CCGC 2 cut(s) 430, 487
AcuI CTGAAG 1 cut(s) 432
AfaI GTAC 2 cut(s) 118, 530
AfiI CCNNNNNNNGG 2 cut(s) 107, 340
AgsI TTSAA 2 cut(s) 281, 581
AjnI CCWGG 2 cut(s) 100, 447
AluBI AGCT 3 cut(s) 57, 62, 605
AluI AGCT 3 cut(s) 57, 62, 605
Alw21I GWGCWC 1 cut(s) 144
Alw26I GTCTC 1 cut(s) 518
AoxI GGCC 4 cut(s) 97, 380, 572, 630
ApeKI GCWGC 1 cut(s) 353
ArsI GACNNNNNNTTYG 2 cut(s) 205, 237
AspS9I GGNCC 3 cut(s) 98, 213, 380
AvaII GGWCC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 144
BbvI GCAGC 1 cut(s) 340
BccI CCATC 3 cut(s) 165, 317, 415
BciT130I CCWGG 2 cut(s) 102, 449
BcoDI GTCTC 1 cut(s) 518
BfmI CTRYAG 2 cut(s) 38, 75
BisI GCNGC 2 cut(s) 354, 431
BlpI GCTNAGC 1 cut(s) 58
BlsI GCNGC 2 cut(s) 355, 432
BmcAI AGTACT 1 cut(s) 530
Bme1390I CCNGG 2 cut(s) 102, 449
Bme18I GGWCC 1 cut(s) 213
BmgT120I GGNCC 3 cut(s) 98, 213, 380
BmiI GGNNCC 2 cut(s) 214, 446
BmrFI CCNGG 2 cut(s) 102, 449
BmsI GCATC 1 cut(s) 553
Bpu1102I GCTNAGC 1 cut(s) 58
BsaJI CCNNGG 2 cut(s) 100, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 107, 340
Bse3DI GCAATG 1 cut(s) 294
BseBI CCWGG 2 cut(s) 102, 449
BseDI CCNNGG 2 cut(s) 100, 448
BseGI GGATG 2 cut(s) 370, 466
BseLI CCNNNNNNNGG 2 cut(s) 107, 340
BseMI GCAATG 1 cut(s) 294
BseXI GCAGC 1 cut(s) 340
BshFI GGCC 4 cut(s) 99, 382, 574, 632
BsiHKAI GWGCWC 1 cut(s) 144
BslFI GGGAC 2 cut(s) 226, 236
BslI CCNNNNNNNGG 2 cut(s) 107, 340
BsmAI GTCTC 1 cut(s) 518
BsmFI GGGAC 2 cut(s) 226, 236
BsnI GGCC 4 cut(s) 99, 382, 574, 632
Bsp1286I GDGCHC 1 cut(s) 144
Bsp143I GATC 2 cut(s) 64, 172
Bsp1720I GCTNAGC 1 cut(s) 58
BspACI CCGC 2 cut(s) 430, 487
BspANI GGCC 4 cut(s) 99, 382, 574, 632
BspLI GGNNCC 2 cut(s) 214, 446
BspMAI CTGCAG 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 294
BssECI CCNNGG 2 cut(s) 100, 448
BssMI GATC 2 cut(s) 64, 172
Bst2UI CCWGG 2 cut(s) 102, 449
Bst4CI ACNGT 2 cut(s) 79, 407
Bst6I CTCTTC 3 cut(s) 411, 448, 576
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 3 cut(s) 58, 161, 179
BstF5I GGATG 2 cut(s) 370, 466
BstKTI GATC 2 cut(s) 67, 175
BstMAI GTCTC 1 cut(s) 518
BstMBI GATC 2 cut(s) 64, 172
BstMWI GCNNNNNNNGC 1 cut(s) 611
BstNI CCWGG 2 cut(s) 102, 449
BstSCI CCNGG 2 cut(s) 100, 447
BstSFI CTRYAG 2 cut(s) 38, 75
BstV1I GCAGC 1 cut(s) 340
BsuRI GGCC 4 cut(s) 99, 382, 574, 632
BtsCI GGATG 2 cut(s) 370, 466
BtsI GCAGTG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 112
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 3 cut(s) 98, 213, 380
Csp6I GTAC 2 cut(s) 117, 529
CviAII CATG 1 cut(s) 217
CviQI GTAC 2 cut(s) 117, 529
DdeI CTNAG 3 cut(s) 58, 161, 179
DpnI GATC 2 cut(s) 66, 174
DpnII GATC 2 cut(s) 64, 172
Eam1104I CTCTTC 3 cut(s) 411, 448, 576
EarI CTCTTC 3 cut(s) 411, 448, 576
Eco147I AGGCCT 1 cut(s) 574
Eco47I GGWCC 1 cut(s) 213
Eco57I CTGAAG 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 100, 447
FaeI CATG 1 cut(s) 220
FaiI YATR 5 cut(s) 51, 218, 230, 470, 591
FaqI GGGAC 2 cut(s) 226, 236
FatI CATG 1 cut(s) 216
Fnu4HI GCNGC 2 cut(s) 354, 431
FokI GGATG 2 cut(s) 377, 473
Fsp4HI GCNGC 2 cut(s) 354, 431
GluI GCNGC 2 cut(s) 354, 431
HaeIII GGCC 4 cut(s) 99, 382, 574, 632
Hin1II CATG 1 cut(s) 220
HindIII AAGCTT 1 cut(s) 55
HinfI GANTC 3 cut(s) 506, 511, 593
Hpy166II GTNNAC 1 cut(s) 153
Hpy188I TCNGA 6 cut(s) 69, 205, 226, 259, 412, 555
Hpy188III TCNNGA 1 cut(s) 278
Hpy8I GTNNAC 1 cut(s) 153
HpyAV CCTTC 7 cut(s) 22, 233, 264, 393, 535, 564, 622
HpyCH4III ACNGT 2 cut(s) 79, 407
HpyCH4V TGCA 4 cut(s) 40, 128, 356, 614
HpyF10VI GCNNNNNNNGC 1 cut(s) 611
HpyF3I CTNAG 3 cut(s) 58, 161, 179
Hsp92II CATG 1 cut(s) 220
Kzo9I GATC 2 cut(s) 64, 172
Lsp1109I GCAGC 1 cut(s) 340
LweI GCATC 1 cut(s) 553
MaeIII GTNAC 3 cut(s) 262, 335, 367
MalI GATC 2 cut(s) 66, 174
MboI GATC 2 cut(s) 64, 172
MboII GAAGA 6 cut(s) 167, 425, 428, 465, 568, 593
MhlI GDGCHC 1 cut(s) 144
MluCI AATT 1 cut(s) 267
MlyI GAGTC 2 cut(s) 520, 602
MnlI CCTC 7 cut(s) 25, 37, 334, 355, 420, 451, 577
MseI TTAA 4 cut(s) 234, 270, 437, 492
MslI CAYNNNNRTG 1 cut(s) 162
MspR9I CCNGG 2 cut(s) 102, 449
MvaI CCWGG 2 cut(s) 102, 449
MwoI GCNNNNNNNGC 1 cut(s) 611
NdeII GATC 2 cut(s) 64, 172
NlaIII CATG 1 cut(s) 220
NlaIV GGNNCC 2 cut(s) 214, 446
NmuCI GTSAC 1 cut(s) 367
PceI AGGCCT 1 cut(s) 574
PfeI GAWTC 1 cut(s) 506
PflMI CCANNNNNTGG 1 cut(s) 107
PkrI GCNGC 2 cut(s) 355, 432
PleI GAGTC 2 cut(s) 519, 601
PpsI GAGTC 2 cut(s) 519, 601
Psp6I CCWGG 2 cut(s) 100, 447
PspGI CCWGG 2 cut(s) 100, 447
PspN4I GGNNCC 2 cut(s) 214, 446
PspPI GGNCC 3 cut(s) 98, 213, 380
PstI CTGCAG 1 cut(s) 42
RsaI GTAC 2 cut(s) 118, 530
RsaNI GTAC 2 cut(s) 117, 529
RseI CAYNNNNRTG 1 cut(s) 162
SaqAI TTAA 4 cut(s) 234, 270, 437, 492
SatI GCNGC 2 cut(s) 354, 431
Sau3AI GATC 2 cut(s) 64, 172
Sau96I GGNCC 3 cut(s) 98, 213, 380
ScaI AGTACT 1 cut(s) 530
SchI GAGTC 2 cut(s) 520, 602
ScrFI CCNGG 2 cut(s) 102, 449
SduI GDGCHC 1 cut(s) 144
SetI ASST 5 cut(s) 59, 64, 498, 588, 607
SfaNI GCATC 1 cut(s) 553
SfcI CTRYAG 2 cut(s) 38, 75
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 1 cut(s) 162
Sse9I AATT 1 cut(s) 267
SseBI AGGCCT 1 cut(s) 574
SsiI CCGC 2 cut(s) 430, 487
StuI AGGCCT 1 cut(s) 574
StyD4I CCNGG 2 cut(s) 100, 447
TaaI ACNGT 2 cut(s) 79, 407
TaqI TCGA 2 cut(s) 509, 539
TasI AATT 1 cut(s) 267
TatI WGTACW 1 cut(s) 528
TauI GCSGC 1 cut(s) 433
TfiI GAWTC 1 cut(s) 506
Tru1I TTAA 4 cut(s) 234, 270, 437, 492
Tru9I TTAA 4 cut(s) 234, 270, 437, 492
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 1 cut(s) 367
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 1 cut(s) 367
TspDTI ATGAA 1 cut(s) 375
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 112
Van91I CCANNNNNTGG 1 cut(s) 107
VpaK11BI GGWCC 1 cut(s) 213
XcmI CCANNNNNNNNNTGG 1 cut(s) 164
ZrmI AGTACT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.