MD07G1110400.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014211
Physical Location & Seq
Forward (+)
12683567 .. 12688395
4829 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1110400.v1.1.491

Sequence Viewer

Length: 711 bp
ATGGAAGGAGATCAAAGAGGGGTTCCGAATTACGAGGTTCAGATTTCGTTTTCGAGCACCCCACAACAAGCGATCCACGAAATGGGGTTTGTGCAGTTCGAAGATCAGCATCATCATAATCAGGTTTTGAGCTTCTTGGCACCCTCATCATCACCTGTACAAGTACAACAACAGCTGAACCCTAATAATTCTCACCATCATCATCAGCCTGCTGCTCTCAGCACCTCCTCCTCCGCCACCGCCACTGCCACCAGCAACGCCGCCACCATGGGGTTTAGTACAAGACCTTCTTGGAATAATAATAACGAGCAGGTGGGAACGTTGGATCCAAAGGGTGTTAGTGATGAAAATGGGACTGGTAATGCTAGTGATTGCAGCAACTCATGGTGGAGGAGTTCAAGCTCAGAGAAAAGCAAGATGAAGGTGAGGAGAAAGCTTAGAGAGCCAAGATTTTGTTTCCAAACCAGAAGTGACGTGGATGTGCTTGATGATGGTTACAAATGGAGGAAATATGGTCAAAAAGTTGTCAAGAACAGCCTTCATCCAAGAAGCTACTACAGGTGTACTCACAATAACTGTCGGGTGAAGAAGAGGGTTGAACGTCTGTCAGAAGACTGTAGAATGGTGATAACAACCTACGAAGGTAGACACAACCACTCCCCTTGTGATGACTCTACCTCCTCTGAACATGAATGCTTTAGCTCTTTCTAG

Protein Analysis

237

Amino Acids

26.78

Weight (kDa)

8.17

Isoelectric Point (pI)

48.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 163 - 219 1.6e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44745
fragaria_vesca FvH4_7g10440 FvH4_7g10440 FvH4_7g10440
malus_domestica MD07G1110400.v1.1
prunus_persica Prupe.2G133800_v2.0.a1 Prupe.2G133800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348121
rosa_laevigata RLG00000028687
rosa_roxburghii Rroxscaffold_4G00307200
rosa_rugosa Rorug01G0192600
rosa_samantha Rh1AG210300 Rh1BG176000 Rh1CG194900 Rh1DG205800
rosa_wichuraiana Rw0G011400 Rw1G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 301
AccB1I GGYRCC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 82
AccI GTMKAC 1 cut(s) 646
AciI CCGC 3 cut(s) 234, 240, 261
AclI AACGTT 1 cut(s) 320
AclWI GGATC 3 cut(s) 67, 320, 333
AfaI GTAC 4 cut(s) 159, 165, 280, 565
AfiI CCNNNNNNNGG 2 cut(s) 82, 270
AgsI TTSAA 2 cut(s) 399, 599
AjiI CACGTC 1 cut(s) 475
AloI GAACNNNNNNTCC 2 cut(s) 310, 342
AluBI AGCT 6 cut(s) 132, 175, 402, 436, 552, 702
AluI AGCT 6 cut(s) 132, 175, 402, 436, 552, 702
Alw21I GWGCWC 1 cut(s) 59
AlwI GGATC 3 cut(s) 67, 320, 333
ApeKI GCWGC 2 cut(s) 212, 375
AsuHPI GGTGA 5 cut(s) 144, 185, 436, 595, 637
AsuII TTCGAA 1 cut(s) 99
BamHI GGATCC 1 cut(s) 325
BanI GGYRCC 1 cut(s) 139
BarI GAAGNNNNNNTAC 2 cut(s) 271, 303
BbsI GAAGAC 1 cut(s) 618
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 2 cut(s) 199, 387
BccI CCATC 2 cut(s) 204, 485
BfaI CTAG 2 cut(s) 366, 709
BfmI CTRYAG 2 cut(s) 556, 616
BfuAI ACCTGC 1 cut(s) 301
BisI GCNGC 3 cut(s) 213, 261, 376
BlsI GCNGC 3 cut(s) 214, 262, 377
BmgBI CACGTC 1 cut(s) 475
BmiI GGNNCC 3 cut(s) 24, 141, 327
BmsI GCATC 1 cut(s) 118
BpiI GAAGAC 1 cut(s) 618
Bpu14I TTCGAA 1 cut(s) 99
BsaBI GATNNNNATC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 267
BsaXI ACNNNNNCTCC 4 cut(s) 641, 662, 671, 692
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 270
Bse1I ACTGG 1 cut(s) 361
Bse8I GATNNNNATC 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 267
BseGI GGATG 2 cut(s) 484, 541
BseJI GATNNNNATC 1 cut(s) 108
BseLI CCNNNNNNNGG 2 cut(s) 82, 270
BseMII CTCAG 2 cut(s) 232, 417
BseNI ACTGG 1 cut(s) 361
BseRI GAGGAG 5 cut(s) 217, 220, 406, 442, 670
BseXI GCAGC 2 cut(s) 199, 387
BsgI GTGCAG 1 cut(s) 113
BshNI GGYRCC 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 59
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 2 cut(s) 82, 270
BsmFI GGGAC 1 cut(s) 367
BsmI GAATGC 1 cut(s) 698
Bsp119I TTCGAA 1 cut(s) 99
Bsp1286I GDGCHC 1 cut(s) 59
Bsp1407I TGTACA 1 cut(s) 157
Bsp143I GATC 4 cut(s) 10, 72, 103, 325
Bsp19I CCATGG 1 cut(s) 267
BspACI CCGC 3 cut(s) 234, 240, 261
BspCNI CTCAG 2 cut(s) 231, 416
BspLI GGNNCC 3 cut(s) 24, 141, 327
BspMI ACCTGC 1 cut(s) 301
BspPI GGATC 3 cut(s) 67, 320, 333
BspT104I TTCGAA 1 cut(s) 99
BspT107I GGYRCC 1 cut(s) 139
BsrGI TGTACA 1 cut(s) 157
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 267
BssMI GATC 4 cut(s) 10, 72, 103, 325
BssT1I CCWWGG 1 cut(s) 267
Bst4CI ACNGT 2 cut(s) 578, 617
Bst6I CTCTTC 1 cut(s) 584
BstAUI TGTACA 1 cut(s) 157
BstBI TTCGAA 1 cut(s) 99
BstC8I GCNNGC 1 cut(s) 210
BstDEI CTNAG 3 cut(s) 218, 403, 437
BstDSI CCRYGG 1 cut(s) 267
BstF5I GGATG 2 cut(s) 484, 541
BstKTI GATC 4 cut(s) 13, 75, 106, 328
BstMBI GATC 4 cut(s) 10, 72, 103, 325
BstMWI GCNNNNNNNGC 1 cut(s) 442
BstSFI CTRYAG 2 cut(s) 556, 616
BstV1I GCAGC 2 cut(s) 199, 387
BstV2I GAAGAC 1 cut(s) 618
BstX2I RGATCY 1 cut(s) 325
BstYI RGATCY 1 cut(s) 325
BtgI CCRYGG 1 cut(s) 267
BtrI CACGTC 1 cut(s) 475
BtsCI GGATG 2 cut(s) 484, 541
BtsI GCAGTG 1 cut(s) 243
BtsIMutI CAGTG 1 cut(s) 243
BveI ACCTGC 1 cut(s) 301
Cac8I GCNNGC 1 cut(s) 210
Csp6I GTAC 4 cut(s) 158, 164, 279, 564
CspCI CAANNNNNGTGG 2 cut(s) 644, 679
CviAII CATG 3 cut(s) 268, 384, 689
CviJI RGCY 9 cut(s) 132, 175, 208, 402, 436, 445, 537, 552, 702
CviKI_1 RGCY 9 cut(s) 132, 175, 208, 402, 436, 445, 537, 552, 702
CviQI GTAC 4 cut(s) 158, 164, 279, 564
DdeI CTNAG 3 cut(s) 218, 403, 437
DpnI GATC 4 cut(s) 12, 74, 105, 327
DpnII GATC 4 cut(s) 10, 72, 103, 325
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
EciI GGCGGA 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 267
EcoT14I CCWWGG 1 cut(s) 267
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 3 cut(s) 271, 387, 692
FaiI YATR 5 cut(s) 117, 269, 385, 513, 690
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FaqI GGGAC 1 cut(s) 367
FatI CATG 3 cut(s) 267, 383, 688
FblI GTMKAC 1 cut(s) 646
Fnu4HI GCNGC 3 cut(s) 213, 261, 376
FokI GGATG 2 cut(s) 491, 528
Fsp4HI GCNGC 3 cut(s) 213, 261, 376
FspBI CTAG 2 cut(s) 366, 709
GluI GCNGC 3 cut(s) 213, 261, 376
Hin1II CATG 3 cut(s) 271, 387, 692
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 1 cut(s) 671
HphI GGTGA 5 cut(s) 144, 185, 436, 595, 637
Hpy166II GTNNAC 2 cut(s) 564, 647
Hpy188I TCNGA 5 cut(s) 27, 42, 406, 610, 685
Hpy188III TCNNGA 1 cut(s) 529
Hpy8I GTNNAC 2 cut(s) 564, 647
HpyAV CCTTC 4 cut(s) 297, 415, 548, 635
HpyCH4III ACNGT 2 cut(s) 578, 617
HpyCH4IV ACGT 3 cut(s) 320, 474, 601
HpyCH4V TGCA 2 cut(s) 94, 375
HpyF10VI GCNNNNNNNGC 1 cut(s) 442
HpyF3I CTNAG 3 cut(s) 218, 403, 437
HpySE526I ACGT 3 cut(s) 320, 474, 601
Hsp92II CATG 3 cut(s) 271, 387, 692
Kzo9I GATC 4 cut(s) 10, 72, 103, 325
LpnPI CCDG 8 cut(s) 107, 168, 222, 265, 296, 342, 478, 544
Lsp1109I GCAGC 2 cut(s) 199, 387
LweI GCATC 1 cut(s) 118
MaeI CTAG 2 cut(s) 366, 709
MaeII ACGT 3 cut(s) 320, 474, 601
MaeIII GTNAC 2 cut(s) 470, 494
MalI GATC 4 cut(s) 12, 74, 105, 327
MboI GATC 4 cut(s) 10, 72, 103, 325
MboII GAAGA 4 cut(s) 113, 598, 601, 623
MflI RGATCY 1 cut(s) 325
MhlI GDGCHC 1 cut(s) 59
MluCI AATT 2 cut(s) 28, 187
MlyI GAGTC 1 cut(s) 665
MmeI TCCRAC 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 175
Mva1269I GAATGC 1 cut(s) 698
MwoI GCNNNNNNNGC 1 cut(s) 442
NcoI CCATGG 1 cut(s) 267
NdeII GATC 4 cut(s) 10, 72, 103, 325
NlaIII CATG 3 cut(s) 271, 387, 692
NlaIV GGNNCC 3 cut(s) 24, 141, 327
NmuCI GTSAC 1 cut(s) 470
NspV TTCGAA 1 cut(s) 99
PaqCI CACCTGC 1 cut(s) 301
PctI GAATGC 1 cut(s) 698
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 3 cut(s) 214, 262, 377
PleI GAGTC 1 cut(s) 665
PpsI GAGTC 1 cut(s) 665
Psp1406I AACGTT 1 cut(s) 320
PspN4I GGNNCC 3 cut(s) 24, 141, 327
PsuI RGATCY 1 cut(s) 325
PvuII CAGCTG 1 cut(s) 175
RsaI GTAC 4 cut(s) 159, 165, 280, 565
RsaNI GTAC 4 cut(s) 158, 164, 279, 564
SatI GCNGC 3 cut(s) 213, 261, 376
Sau3AI GATC 4 cut(s) 10, 72, 103, 325
SchI GAGTC 1 cut(s) 665
SduI GDGCHC 1 cut(s) 59
SfaNI GCATC 1 cut(s) 118
SfcI CTRYAG 2 cut(s) 556, 616
SfuI TTCGAA 1 cut(s) 99
Sse9I AATT 2 cut(s) 28, 187
SsiI CCGC 3 cut(s) 234, 240, 261
SspMI CTAG 2 cut(s) 366, 709
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 578, 617
TaiI ACGT 3 cut(s) 323, 477, 604
TaqI TCGA 2 cut(s) 53, 99
TasI AATT 2 cut(s) 28, 187
TatI WGTACW 4 cut(s) 157, 163, 278, 563
TauI GCSGC 1 cut(s) 263
TscAI CASTG 1 cut(s) 250
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 212, 375
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 4 cut(s) 360, 434, 530, 705
TspRI CASTG 1 cut(s) 250
Van91I CCANNNNNTGG 1 cut(s) 82
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XmiI GTMKAC 1 cut(s) 646
XspI CTAG 2 cut(s) 366, 709
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.