RchiOBHm_Chr1g0348121
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0014211
Physical Location & Seq
Forward (+)
40913616 .. 40918500
4885 bp
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UTR
Exon/CDS
Intron
PRQ57418

Sequence Viewer

Length: 894 bp
ATGGAAGGAGGTCATCACAGAGGGGTTTCAAATTACGATCTTCATCAGCAGGTTTCGTTTTCCAGCAGTACCCCTCCCGCAGAACAAGTGATCCACCACGAATTGGGGTTCGTGCAGTTTGAAAATCATCATCATCATAATCAAGTTTTGAGCTTCTTGGCACCGTCTTCCGCACAACAGTCACAGCCACCGCCGCAGCAGCAGCAGCAGCAGCCTTCTTCTCACCATCATCAGACAGCTGCCGTGAACAGTAGCATCACTGTCACCACCACCGCTGCGACAAACACCAACATGGGGTTTAGTCACAGTCATGATCTTGTTACAAGACCTTCTTGGAATAACAACGACCAGGTGGGAACGCTGGATCCGAAGGGTGTCAGTGATGAAAACGGAACCGGTAATGGTAGCGATTGCAGCAATTCATGGTGGAGGAGCTCAAACTCAGAGAAAAGCAAGATGAAGGTGAGGAGAAAGCTCAGAGAGCCAAGATTCTGTTTCCAAACCAGGAGTGATGTGGATGTGCTTGATGATGGTTACAAGTGGAGGAAATATGGCCAGAAAGTTGTCAAGAACAGCCTTCATCCTAGAAGTTACTACCGGTGTACTCACAGTAACTGTCGGGTGAAGAAAAGGGTTGAACGACTGTCTGAAGATTGTCGTATGGTGATAACAACCTATGAAGGACAATTCCATTATCTTCCAAGTATTGAAGCAGTACGCAGATATTCTACGTACTGGACTCCATCTAACACAAACCAAGCCTTGGACTTTCCTGCACTAACAAGTAAAACGCTTCTCCTACGTACTGATAATGGCTGGAGAAAGCAGATACCATGTTACTCTTCTTGCCGCTACAAAATAGTAGTGTTGTTGGTGCATTTTCTGATACTATGA

Protein Analysis

297

Amino Acids

34.07

Weight (kDa)

9.31

Isoelectric Point (pI)

42.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 176 - 229 1.5e-22 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44745
fragaria_vesca FvH4_7g10440 FvH4_7g10440 FvH4_7g10440
malus_domestica MD07G1110400.v1.1
prunus_persica Prupe.2G133800_v2.0.a1 Prupe.2G133800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348121
rosa_laevigata RLG00000028687
rosa_roxburghii Rroxscaffold_4G00307200
rosa_rugosa Rorug01G0192600
rosa_samantha Rh1AG210300 Rh1BG176000 Rh1CG194900 Rh1DG205800
rosa_wichuraiana Rw0G011400 Rw1G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 160
AccB7I CCANNNNNTGG 2 cut(s) 103, 763
AciI CCGC 6 cut(s) 78, 171, 191, 194, 273, 850
AclWI GGATC 3 cut(s) 85, 359, 372
AcoI YGGCCR 1 cut(s) 553
AcuI CTGAAG 1 cut(s) 669
AfaI GTAC 5 cut(s) 70, 604, 717, 734, 805
AfiI CCNNNNNNNGG 3 cut(s) 103, 294, 763
AgeI ACCGGT 2 cut(s) 395, 597
AgsI TTSAA 4 cut(s) 30, 122, 638, 710
AjnI CCWGG 2 cut(s) 348, 503
AloI GAACNNNNNNTCC 4 cut(s) 75, 107, 349, 381
AluBI AGCT 4 cut(s) 153, 239, 435, 475
AluI AGCT 4 cut(s) 153, 239, 435, 475
Alw21I GWGCWC 1 cut(s) 437
AlwI GGATC 3 cut(s) 85, 359, 372
AlwNI CAGNNNCTG 1 cut(s) 615
AoxI GGCC 1 cut(s) 553
ApeKI GCWGC 9 cut(s) 196, 199, 202, 205, 208, 211, 239, 275, 414
AsiGI ACCGGT 2 cut(s) 395, 597
AsuHPI GGTGA 5 cut(s) 215, 256, 475, 634, 676
BalI TGGCCA 1 cut(s) 555
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 437
BbsI GAAGAC 1 cut(s) 159
Bbv12I GWGCWC 1 cut(s) 437
BbvI GCAGC 9 cut(s) 208, 211, 214, 217, 220, 223, 226, 262, 426
BccI CCATC 3 cut(s) 234, 524, 751
BceAI ACGGC 1 cut(s) 227
BciT130I CCWGG 2 cut(s) 350, 505
BfaI CTAG 1 cut(s) 585
BfuAI ACCTGC 1 cut(s) 40
Bme1390I CCNGG 2 cut(s) 350, 505
BmiI GGNNCC 3 cut(s) 162, 366, 394
BmrFI CCNGG 2 cut(s) 350, 505
BmsI GCATC 1 cut(s) 264
BpiI GAAGAC 1 cut(s) 159
BpmI CTGGAG 1 cut(s) 838
BsaAI YACGTR 2 cut(s) 732, 803
BsaBI GATNNNNATC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 762
BsaWI WCCGGW 2 cut(s) 395, 597
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 294, 763
Bse118I RCCGGY 2 cut(s) 395, 597
Bse1I ACTGG 1 cut(s) 740
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 350, 505
BseDI CCNNGG 1 cut(s) 762
BseGI GGATG 2 cut(s) 523, 580
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 3 cut(s) 103, 294, 763
BseMII CTCAG 2 cut(s) 456, 490
BseNI ACTGG 1 cut(s) 740
BseRI GAGGAG 2 cut(s) 445, 481
BseXI GCAGC 9 cut(s) 208, 211, 214, 217, 220, 223, 226, 262, 426
BsgI GTGCAG 2 cut(s) 134, 759
BshFI GGCC 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 2 cut(s) 395, 597
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 2 cut(s) 396, 598
BslI CCNNNNNNNGG 3 cut(s) 103, 294, 763
BsnI GGCC 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 4 cut(s) 37, 90, 313, 364
BspACI CCGC 6 cut(s) 78, 171, 191, 194, 273, 850
BspANI GGCC 1 cut(s) 555
BspCNI CTCAG 2 cut(s) 455, 489
BspHI TCATGA 1 cut(s) 310
BspLI GGNNCC 3 cut(s) 162, 366, 394
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 85, 359, 372
BspT107I GGYRCC 1 cut(s) 160
BsrFI RCCGGY 2 cut(s) 395, 597
BsrI ACTGG 1 cut(s) 740
BssAI RCCGGY 2 cut(s) 395, 597
BssECI CCNNGG 1 cut(s) 762
BssMI GATC 4 cut(s) 37, 90, 313, 364
BssT1I CCWWGG 1 cut(s) 762
Bst2UI CCWGG 2 cut(s) 350, 505
Bst4CI ACNGT 8 cut(s) 165, 180, 251, 262, 308, 611, 617, 645
Bst6I CTCTTC 1 cut(s) 847
BstBAI YACGTR 2 cut(s) 732, 803
BstDEI CTNAG 2 cut(s) 442, 476
BstF5I GGATG 2 cut(s) 523, 580
BstKTI GATC 4 cut(s) 40, 93, 316, 367
BstMBI GATC 4 cut(s) 37, 90, 313, 364
BstMWI GCNNNNNNNGC 8 cut(s) 193, 199, 202, 205, 208, 211, 414, 481
BstNI CCWGG 2 cut(s) 350, 505
BstSCI CCNGG 2 cut(s) 348, 503
BstSNI TACGTA 2 cut(s) 732, 803
BstV1I GCAGC 9 cut(s) 208, 211, 214, 217, 220, 223, 226, 262, 426
BstV2I GAAGAC 1 cut(s) 159
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 1 cut(s) 555
BtsCI GGATG 2 cut(s) 523, 580
BtsIMutI CAGTG 2 cut(s) 258, 385
BveI ACCTGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 615
CciI TCATGA 1 cut(s) 310
Cfr10I RCCGGY 2 cut(s) 395, 597
CsiI ACCWGGT 1 cut(s) 348
Csp6I GTAC 5 cut(s) 69, 603, 716, 733, 804
CspAI ACCGGT 2 cut(s) 395, 597
CviAII CATG 4 cut(s) 292, 311, 423, 834
CviQI GTAC 5 cut(s) 69, 603, 716, 733, 804
DdeI CTNAG 2 cut(s) 442, 476
DpnI GATC 4 cut(s) 39, 92, 315, 366
DpnII GATC 4 cut(s) 37, 90, 313, 364
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 847
Ecl136II GAGCTC 1 cut(s) 435
Eco105I TACGTA 2 cut(s) 732, 803
Eco130I CCWWGG 1 cut(s) 762
Eco24I GRGCYC 1 cut(s) 437
Eco53kI GAGCTC 1 cut(s) 435
Eco57I CTGAAG 1 cut(s) 669
EcoICRI GAGCTC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 348, 503
EcoT14I CCWWGG 1 cut(s) 762
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 762
FaeI CATG 4 cut(s) 295, 314, 426, 837
FaiI YATR 9 cut(s) 138, 293, 312, 424, 552, 662, 678, 835, 892
FalI AAGNNNNNCTT 2 cut(s) 316, 348
FatI CATG 4 cut(s) 291, 310, 422, 833
FauI CCCGC 1 cut(s) 85
FokI GGATG 2 cut(s) 530, 567
FriOI GRGCYC 1 cut(s) 437
FspBI CTAG 1 cut(s) 585
GsuI CTGGAG 1 cut(s) 838
HaeIII GGCC 1 cut(s) 555
HapII CCGG 2 cut(s) 396, 598
Hin1II CATG 4 cut(s) 295, 314, 426, 837
HinfI GANTC 2 cut(s) 489, 739
HpaII CCGG 2 cut(s) 396, 598
HphI GGTGA 5 cut(s) 215, 256, 475, 634, 676
Hpy166II GTNNAC 2 cut(s) 247, 603
Hpy188I TCNGA 6 cut(s) 234, 369, 445, 479, 649, 885
Hpy188III TCNNGA 2 cut(s) 311, 568
Hpy8I GTNNAC 2 cut(s) 247, 603
HpyAV CCTTC 6 cut(s) 225, 339, 364, 454, 587, 674
HpyCH4III ACNGT 8 cut(s) 165, 180, 251, 262, 308, 611, 617, 645
HpyCH4IV ACGT 2 cut(s) 731, 802
HpyCH4V TGCA 4 cut(s) 115, 414, 776, 877
HpyF10VI GCNNNNNNNGC 8 cut(s) 193, 199, 202, 205, 208, 211, 414, 481
HpyF3I CTNAG 2 cut(s) 442, 476
HpySE526I ACGT 2 cut(s) 731, 802
Hsp92II CATG 4 cut(s) 295, 314, 426, 837
Kzo9I GATC 4 cut(s) 37, 90, 313, 364
LmnI GCTCC 1 cut(s) 432
Lsp1109I GCAGC 9 cut(s) 208, 211, 214, 217, 220, 223, 226, 262, 426
LweI GCATC 1 cut(s) 264
MabI ACCWGGT 1 cut(s) 348
MaeI CTAG 1 cut(s) 585
MaeII ACGT 2 cut(s) 731, 802
MaeIII GTNAC 8 cut(s) 180, 262, 302, 319, 533, 590, 611, 836
MalI GATC 4 cut(s) 39, 92, 315, 366
MboI GATC 4 cut(s) 37, 90, 313, 364
MboII GAAGA 7 cut(s) 32, 159, 210, 637, 662, 689, 834
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 437
MlsI TGGCCA 1 cut(s) 555
MluCI AATT 4 cut(s) 31, 101, 418, 686
MluNI TGGCCA 1 cut(s) 555
MlyI GAGTC 1 cut(s) 733
MnlI CCTC 5 cut(s) 14, 84, 423, 459, 537
Mox20I TGGCCA 1 cut(s) 555
MscI TGGCCA 1 cut(s) 555
MslI CAYNNNNRTG 2 cut(s) 290, 309
Msp20I TGGCCA 1 cut(s) 555
MspA1I CMGCKG 2 cut(s) 239, 275
MspI CCGG 2 cut(s) 396, 598
MspR9I CCNGG 2 cut(s) 350, 505
MvaI CCWGG 2 cut(s) 350, 505
MwoI GCNNNNNNNGC 8 cut(s) 193, 199, 202, 205, 208, 211, 414, 481
NdeII GATC 4 cut(s) 37, 90, 313, 364
NlaIII CATG 4 cut(s) 295, 314, 426, 837
NlaIV GGNNCC 3 cut(s) 162, 366, 394
NmuCI GTSAC 3 cut(s) 180, 262, 302
PagI TCATGA 1 cut(s) 310
PcsI WCGNNNNNNNCGW 1 cut(s) 365
PfeI GAWTC 1 cut(s) 489
PflMI CCANNNNNTGG 2 cut(s) 103, 763
PinAI ACCGGT 2 cut(s) 395, 597
PleI GAGTC 1 cut(s) 733
PpsI GAGTC 1 cut(s) 733
Ppu21I YACGTR 2 cut(s) 732, 803
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 348, 503
PspGI CCWGG 2 cut(s) 348, 503
PspN4I GGNNCC 3 cut(s) 162, 366, 394
PstNI CAGNNNCTG 1 cut(s) 615
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 5 cut(s) 70, 604, 717, 734, 805
RsaNI GTAC 5 cut(s) 69, 603, 716, 733, 804
RseI CAYNNNNRTG 2 cut(s) 290, 309
SacI GAGCTC 1 cut(s) 437
Sau3AI GATC 4 cut(s) 37, 90, 313, 364
SchI GAGTC 1 cut(s) 733
ScrFI CCNGG 2 cut(s) 350, 505
SduI GDGCHC 1 cut(s) 437
SexAI ACCWGGT 1 cut(s) 348
SfaNI GCATC 1 cut(s) 264
SmiMI CAYNNNNRTG 2 cut(s) 290, 309
SnaBI TACGTA 2 cut(s) 732, 803
Sse9I AATT 4 cut(s) 31, 101, 418, 686
SsiI CCGC 6 cut(s) 78, 171, 191, 194, 273, 850
SspMI CTAG 1 cut(s) 585
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 348, 503
StyI CCWWGG 1 cut(s) 762
TaaI ACNGT 8 cut(s) 165, 180, 251, 262, 308, 611, 617, 645
TaiI ACGT 2 cut(s) 734, 805
TasI AATT 4 cut(s) 31, 101, 418, 686
TatI WGTACW 1 cut(s) 602
TauI GCSGC 2 cut(s) 196, 852
TfiI GAWTC 1 cut(s) 489
TscAI CASTG 2 cut(s) 265, 385
TseFI GTSAC 3 cut(s) 180, 262, 302
TseI GCWGC 9 cut(s) 196, 199, 202, 205, 208, 211, 239, 275, 414
Tsp45I GTSAC 3 cut(s) 180, 262, 302
TspDTI ATGAA 6 cut(s) 32, 399, 411, 473, 569, 693
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 2 cut(s) 265, 385
Van91I CCANNNNNTGG 2 cut(s) 103, 763
XcmI CCANNNNNNNNNTGG 1 cut(s) 511
XspI CTAG 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.