RLG00000028687
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0014211
Physical Location & Seq
Reverse (-)
26324964 .. 26327965
3002 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028687

Sequence Viewer

Length: 738 bp
ATGGAAGGAGGTCATCACAGAGGGGTTTTAAATTACGATCTTCATCAGCAGGTTTCGTTTTCCAGCAGTACCCCTCCCGCAGAACAAGCGATCCATCACGAATTGGGGTTCGTGCAGTTTGAAAATCATCATCATCATAATCAAGTTTTGAGCTTCTTGGCACCGTCTCCCGCACAACAGTCACAGCCACCGCCGCAGCAGCCTTCTTCTCACCATCATCAGACAGCTGCCGTGAACAGTAGCATCACTGTCACCACCACCGCTGCGACAAACACCAACATGGGGTTTAGTCACGGTCATGATCTTGTTACAAGACCTTCTTGGAATAACAACGACCAGGTGGGAACGCTGGATCCGAAGGGTGTCAGTGATGAAAACGGAACCGGTAATGGTAGCGATTGCAGCAATTCATGGTGGAGAAGCTCAAACTCAGAGAAAAGCAAGATGAAGGTGAGGAGAAAGCTCAGAGAGCCAAGATTCTGTTTCCAAACCAGGAGTGATGTGGATGTGCTTGATGATGGTTATAAGTGGAGGAAATATGGCCAGAAAGTTGTCAAGAACAGCCTTCATCCTAGAAGTTACTACCGGTGTACTCACAATAACTGTCGGGTGAAGAAGAGGGTTGAACGACTGTCTGAAGATTGTCGTATGGTGATAACAACCTATGAAGGTAGACACAACCATATCCCTTCAGATGACTCTAATTCATCTGAGCATGAATGCTTTACCTCTTTCTAA

Protein Analysis

246

Amino Acids

27.86

Weight (kDa)

8.23

Isoelectric Point (pI)

47.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 172 - 228 1.9e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44745
fragaria_vesca FvH4_7g10440 FvH4_7g10440 FvH4_7g10440
malus_domestica MD07G1110400.v1.1
prunus_persica Prupe.2G133800_v2.0.a1 Prupe.2G133800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348121
rosa_laevigata RLG00000028687
rosa_roxburghii Rroxscaffold_4G00307200
rosa_rugosa Rorug01G0192600
rosa_samantha Rh1AG210300 Rh1BG176000 Rh1CG194900 Rh1DG205800
rosa_wichuraiana Rw0G011400 Rw1G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 525
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 160
AccI GTMKAC 1 cut(s) 673
AciI CCGC 5 cut(s) 78, 171, 191, 194, 261
AclWI GGATC 3 cut(s) 85, 347, 360
AcoI YGGCCR 1 cut(s) 541
AcuI CTGAAG 2 cut(s) 657, 675
AfaI GTAC 2 cut(s) 70, 592
AfiI CCNNNNNNNGG 1 cut(s) 282
AgeI ACCGGT 2 cut(s) 383, 585
AgsI TTSAA 2 cut(s) 122, 626
AjnI CCWGG 2 cut(s) 336, 491
AloI GAACNNNNNNTCC 4 cut(s) 75, 107, 337, 369
AluBI AGCT 4 cut(s) 153, 227, 423, 463
AluI AGCT 4 cut(s) 153, 227, 423, 463
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 3 cut(s) 85, 347, 360
AoxI GGCC 1 cut(s) 541
ApeKI GCWGC 5 cut(s) 196, 199, 227, 263, 402
AsiGI ACCGGT 2 cut(s) 383, 585
AsuHPI GGTGA 5 cut(s) 203, 244, 463, 622, 664
BalI TGGCCA 1 cut(s) 543
BamHI GGATCC 1 cut(s) 352
BanI GGYRCC 1 cut(s) 160
BbvI GCAGC 5 cut(s) 208, 211, 214, 250, 414
BccI CCATC 3 cut(s) 102, 222, 512
BceAI ACGGC 1 cut(s) 215
BciT130I CCWGG 2 cut(s) 338, 493
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 573
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 6 cut(s) 194, 197, 200, 228, 264, 403
BlsI GCNGC 6 cut(s) 195, 198, 201, 229, 265, 404
Bme1390I CCNGG 2 cut(s) 338, 493
BmiI GGNNCC 3 cut(s) 162, 354, 382
BmrFI CCNGG 2 cut(s) 338, 493
BmsI GCATC 1 cut(s) 252
BsaBI GATNNNNATC 1 cut(s) 42
BsaWI WCCGGW 2 cut(s) 383, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 282
Bse118I RCCGGY 2 cut(s) 383, 585
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 338, 493
BseGI GGATG 2 cut(s) 511, 568
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 1 cut(s) 282
BseMII CTCAG 3 cut(s) 444, 478, 702
BseRI GAGGAG 1 cut(s) 469
BseXI GCAGC 5 cut(s) 208, 211, 214, 250, 414
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 1 cut(s) 543
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 2 cut(s) 383, 585
BsiSI CCGG 2 cut(s) 384, 586
BslI CCNNNNNNNGG 1 cut(s) 282
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsmI GAATGC 1 cut(s) 725
BsnI GGCC 1 cut(s) 543
Bsp143I GATC 4 cut(s) 37, 90, 301, 352
BspACI CCGC 5 cut(s) 78, 171, 191, 194, 261
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 3 cut(s) 443, 477, 703
BspHI TCATGA 1 cut(s) 298
BspLI GGNNCC 3 cut(s) 162, 354, 382
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 85, 347, 360
BspT107I GGYRCC 1 cut(s) 160
BsrFI RCCGGY 2 cut(s) 383, 585
BssAI RCCGGY 2 cut(s) 383, 585
BssMI GATC 4 cut(s) 37, 90, 301, 352
Bst2UI CCWGG 2 cut(s) 338, 493
Bst4CI ACNGT 7 cut(s) 165, 180, 239, 250, 296, 605, 633
Bst6I CTCTTC 1 cut(s) 611
BstDEI CTNAG 3 cut(s) 430, 464, 711
BstF5I GGATG 2 cut(s) 511, 568
BstKTI GATC 4 cut(s) 40, 93, 304, 355
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 4 cut(s) 37, 90, 301, 352
BstMWI GCNNNNNNNGC 5 cut(s) 86, 193, 199, 402, 469
BstNI CCWGG 2 cut(s) 338, 493
BstSCI CCNGG 2 cut(s) 336, 491
BstV1I GCAGC 5 cut(s) 208, 211, 214, 250, 414
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 543
BtsCI GGATG 2 cut(s) 511, 568
BtsIMutI CAGTG 2 cut(s) 246, 373
BveI ACCTGC 1 cut(s) 40
CciI TCATGA 1 cut(s) 298
Cfr10I RCCGGY 2 cut(s) 383, 585
CsiI ACCWGGT 1 cut(s) 336
Csp6I GTAC 2 cut(s) 69, 591
CspAI ACCGGT 2 cut(s) 383, 585
CviAII CATG 4 cut(s) 280, 299, 411, 716
CviJI RGCY 9 cut(s) 153, 187, 202, 227, 423, 463, 472, 543, 564
CviKI_1 RGCY 9 cut(s) 153, 187, 202, 227, 423, 463, 472, 543, 564
CviQI GTAC 2 cut(s) 69, 591
DdeI CTNAG 3 cut(s) 430, 464, 711
DpnI GATC 4 cut(s) 39, 92, 303, 354
DpnII GATC 4 cut(s) 37, 90, 301, 352
DraI TTTAAA 1 cut(s) 30
EaeI YGGCCR 1 cut(s) 541
Eam1104I CTCTTC 1 cut(s) 611
EarI CTCTTC 1 cut(s) 611
Eco57I CTGAAG 2 cut(s) 657, 675
EcoRII CCWGG 2 cut(s) 336, 491
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 4 cut(s) 283, 302, 414, 719
FalI AAGNNNNNCTT 2 cut(s) 304, 336
FatI CATG 4 cut(s) 279, 298, 410, 715
FauI CCCGC 2 cut(s) 85, 178
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 6 cut(s) 194, 197, 200, 228, 264, 403
FokI GGATG 2 cut(s) 518, 555
Fsp4HI GCNGC 6 cut(s) 194, 197, 200, 228, 264, 403
FspBI CTAG 1 cut(s) 573
GluI GCNGC 6 cut(s) 194, 197, 200, 228, 264, 403
HaeIII GGCC 1 cut(s) 543
HapII CCGG 2 cut(s) 384, 586
Hin1II CATG 4 cut(s) 283, 302, 414, 719
HinfI GANTC 2 cut(s) 477, 698
HpaII CCGG 2 cut(s) 384, 586
HphI GGTGA 5 cut(s) 203, 244, 463, 622, 664
Hpy166II GTNNAC 3 cut(s) 235, 591, 674
Hpy188I TCNGA 7 cut(s) 222, 357, 433, 467, 637, 694, 712
Hpy188III TCNNGA 3 cut(s) 98, 299, 556
Hpy8I GTNNAC 3 cut(s) 235, 591, 674
HpyAV CCTTC 7 cut(s) 213, 327, 352, 442, 575, 662, 699
HpyCH4III ACNGT 7 cut(s) 165, 180, 239, 250, 296, 605, 633
HpyCH4V TGCA 2 cut(s) 115, 402
HpyF10VI GCNNNNNNNGC 5 cut(s) 86, 193, 199, 402, 469
HpyF3I CTNAG 3 cut(s) 430, 464, 711
Hsp92II CATG 4 cut(s) 283, 302, 414, 719
Kzo9I GATC 4 cut(s) 37, 90, 301, 352
Lsp1109I GCAGC 5 cut(s) 208, 211, 214, 250, 414
LweI GCATC 1 cut(s) 252
MabI ACCWGGT 1 cut(s) 336
MaeI CTAG 1 cut(s) 573
MaeIII GTNAC 5 cut(s) 180, 250, 290, 307, 578
MalI GATC 4 cut(s) 39, 92, 303, 354
MboI GATC 4 cut(s) 37, 90, 301, 352
MboII GAAGA 5 cut(s) 32, 198, 625, 628, 650
MflI RGATCY 1 cut(s) 352
MlsI TGGCCA 1 cut(s) 543
MluCI AATT 4 cut(s) 31, 101, 406, 703
MluNI TGGCCA 1 cut(s) 543
MlyI GAGTC 1 cut(s) 692
MnlI CCTC 5 cut(s) 14, 84, 447, 525, 612
Mox20I TGGCCA 1 cut(s) 543
MscI TGGCCA 1 cut(s) 543
MseI TTAA 1 cut(s) 29
MslI CAYNNNNRTG 2 cut(s) 278, 297
Msp20I TGGCCA 1 cut(s) 543
MspA1I CMGCKG 2 cut(s) 227, 263
MspI CCGG 2 cut(s) 384, 586
MspR9I CCNGG 2 cut(s) 338, 493
Mva1269I GAATGC 1 cut(s) 725
MvaI CCWGG 2 cut(s) 338, 493
MwoI GCNNNNNNNGC 5 cut(s) 86, 193, 199, 402, 469
NdeII GATC 4 cut(s) 37, 90, 301, 352
NlaIII CATG 4 cut(s) 283, 302, 414, 719
NlaIV GGNNCC 3 cut(s) 162, 354, 382
NmuCI GTSAC 3 cut(s) 180, 250, 290
PagI TCATGA 1 cut(s) 298
PcsI WCGNNNNNNNCGW 1 cut(s) 353
PctI GAATGC 1 cut(s) 725
PfeI GAWTC 1 cut(s) 477
PinAI ACCGGT 2 cut(s) 383, 585
PkrI GCNGC 6 cut(s) 195, 198, 201, 229, 265, 404
PleI GAGTC 1 cut(s) 692
PpsI GAGTC 1 cut(s) 692
PsiI TTATAA 1 cut(s) 525
Psp6I CCWGG 2 cut(s) 336, 491
PspGI CCWGG 2 cut(s) 336, 491
PspN4I GGNNCC 3 cut(s) 162, 354, 382
PsuI RGATCY 1 cut(s) 352
PvuII CAGCTG 1 cut(s) 227
RsaI GTAC 2 cut(s) 70, 592
RsaNI GTAC 2 cut(s) 69, 591
RseI CAYNNNNRTG 2 cut(s) 278, 297
SaqAI TTAA 1 cut(s) 29
SatI GCNGC 6 cut(s) 194, 197, 200, 228, 264, 403
Sau3AI GATC 4 cut(s) 37, 90, 301, 352
SchI GAGTC 1 cut(s) 692
ScrFI CCNGG 2 cut(s) 338, 493
SexAI ACCWGGT 1 cut(s) 336
SfaNI GCATC 1 cut(s) 252
SmiMI CAYNNNNRTG 2 cut(s) 278, 297
Sse9I AATT 4 cut(s) 31, 101, 406, 703
SsiI CCGC 5 cut(s) 78, 171, 191, 194, 261
SspMI CTAG 1 cut(s) 573
StyD4I CCNGG 2 cut(s) 336, 491
TaaI ACNGT 7 cut(s) 165, 180, 239, 250, 296, 605, 633
TasI AATT 4 cut(s) 31, 101, 406, 703
TatI WGTACW 1 cut(s) 590
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 1 cut(s) 477
Tru1I TTAA 1 cut(s) 29
Tru9I TTAA 1 cut(s) 29
TscAI CASTG 2 cut(s) 253, 373
TseFI GTSAC 3 cut(s) 180, 250, 290
TseI GCWGC 5 cut(s) 196, 199, 227, 263, 402
Tsp45I GTSAC 3 cut(s) 180, 250, 290
TspDTI ATGAA 8 cut(s) 32, 387, 399, 461, 557, 681, 696, 732
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 2 cut(s) 253, 373
XcmI CCANNNNNNNNNTGG 1 cut(s) 499
XmiI GTMKAC 1 cut(s) 673
XspI CTAG 1 cut(s) 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.