Rh1BG176000
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0014211
Physical Location & Seq
Forward (+)
28028141 .. 28030807
2667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG176000.1

Sequence Viewer

Length: 699 bp
ATGGAAGGAGGTCATCACAGAGGGGTTTCAAATTACGATCTTCATCAGCAGGTTTCGTTTTCCAGCAGTAACCCTCCCGCAGAACAAGCGATCCACCACGAATTGGGGTTCGTGCAGTTTGAAAATCATCATCATCATAATCAAGTTTTGAGCTTCTTGGCACCGTCTCCCGCACAACAGTCACAGCCACCGCCGCAGCAGCAGCAGCAGCCTTCTTCTCACCATCATCAGACAGCTGCCGTGAACAGTAGCATCACTGTCACCACCACCGCTGCGACAAACACCAACATGGGGTTTAGTCACAGTCATGATCTTGTTACAAGACCTTCTTGGAATAACAACGACCAGGTGGGAACGCTGGATCCGAAGGGTGTCAGTGATGAAAACGGAACCGGTAATGGTAGCGATTGCAGCAATTCATGGTGGAGGAGCTCAAACTCAGAGAAAAGCAAGATGAAGGTGAGGAGAAAGCTCAGAGAGCCAAGATTCTGTTTCCAAACCAGGAGTGATGTGGATGTGCTTGATGATGGTTACAAGTGGAGGAAATATGGCCAGAAAGTTGTCAAGAACAGCCTTCATCCTAGGTACGTTATATTGCTTATTTTATTTCTTAATTTGTTTTTTTTTTTAAATGACTTGGTAGTTGGTACACATCAAATTGATTACCAAACTAGACAATTTGTAAATAGTTTGTGTTAA

Protein Analysis

232

Amino Acids

26.34

Weight (kDa)

7.88

Isoelectric Point (pI)

38.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 175 - 196 8.8e-07 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44745
fragaria_vesca FvH4_7g10440 FvH4_7g10440 FvH4_7g10440
malus_domestica MD07G1110400.v1.1
prunus_persica Prupe.2G133800_v2.0.a1 Prupe.2G133800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348121
rosa_laevigata RLG00000028687
rosa_roxburghii Rroxscaffold_4G00307200
rosa_rugosa Rorug01G0192600
rosa_samantha Rh1AG210300 Rh1BG176000 Rh1CG194900 Rh1DG205800
rosa_wichuraiana Rw0G011400 Rw1G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 5 cut(s) 78, 171, 191, 194, 270
AclWI GGATC 3 cut(s) 85, 356, 369
AcoI YGGCCR 1 cut(s) 550
AfaI GTAC 2 cut(s) 587, 649
AfiI CCNNNNNNNGG 2 cut(s) 103, 291
AgeI ACCGGT 1 cut(s) 392
AgsI TTSAA 2 cut(s) 30, 122
AjnI CCWGG 2 cut(s) 345, 500
AloI GAACNNNNNNTCC 4 cut(s) 75, 107, 346, 378
AluBI AGCT 4 cut(s) 153, 236, 432, 472
AluI AGCT 4 cut(s) 153, 236, 432, 472
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 3 cut(s) 85, 356, 369
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 8 cut(s) 196, 199, 202, 205, 208, 236, 272, 411
AsiGI ACCGGT 1 cut(s) 392
AspA2I CCTAGG 1 cut(s) 581
AsuHPI GGTGA 3 cut(s) 212, 253, 472
AvrII CCTAGG 1 cut(s) 581
BalI TGGCCA 1 cut(s) 552
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 8 cut(s) 208, 211, 214, 217, 220, 223, 259, 423
BccI CCATC 2 cut(s) 231, 521
BceAI ACGGC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 347, 502
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 2 cut(s) 582, 672
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
BlnI CCTAGG 1 cut(s) 581
BlsI GCNGC 9 cut(s) 195, 198, 201, 204, 207, 210, 238, 274, 413
Bme1390I CCNGG 2 cut(s) 347, 502
BmiI GGNNCC 3 cut(s) 162, 363, 391
BmrFI CCNGG 2 cut(s) 347, 502
BmsI GCATC 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 42
BsaJI CCNNGG 1 cut(s) 581
BsaWI WCCGGW 1 cut(s) 392
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 291
Bse118I RCCGGY 1 cut(s) 392
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 347, 502
BseDI CCNNGG 1 cut(s) 581
BseGI GGATG 2 cut(s) 520, 577
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 103, 291
BseMII CTCAG 2 cut(s) 453, 487
BseRI GAGGAG 2 cut(s) 442, 478
BseXI GCAGC 8 cut(s) 208, 211, 214, 217, 220, 223, 259, 423
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 1 cut(s) 552
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 1 cut(s) 392
BsiHKAI GWGCWC 1 cut(s) 434
BsiSI CCGG 1 cut(s) 393
BslI CCNNNNNNNGG 2 cut(s) 103, 291
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsnI GGCC 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 4 cut(s) 37, 90, 310, 361
BspACI CCGC 5 cut(s) 78, 171, 191, 194, 270
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 2 cut(s) 452, 486
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 3 cut(s) 162, 363, 391
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 85, 356, 369
BspT107I GGYRCC 1 cut(s) 160
BsrFI RCCGGY 1 cut(s) 392
BssAI RCCGGY 1 cut(s) 392
BssECI CCNNGG 1 cut(s) 581
BssMI GATC 4 cut(s) 37, 90, 310, 361
BssT1I CCWWGG 1 cut(s) 581
Bst2UI CCWGG 2 cut(s) 347, 502
Bst4CI ACNGT 5 cut(s) 165, 180, 248, 259, 305
BstDEI CTNAG 2 cut(s) 439, 473
BstF5I GGATG 2 cut(s) 520, 577
BstKTI GATC 4 cut(s) 40, 93, 313, 364
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 4 cut(s) 37, 90, 310, 361
BstMWI GCNNNNNNNGC 8 cut(s) 86, 193, 199, 202, 205, 208, 411, 478
BstNI CCWGG 2 cut(s) 347, 502
BstSCI CCNGG 2 cut(s) 345, 500
BstV1I GCAGC 8 cut(s) 208, 211, 214, 217, 220, 223, 259, 423
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 552
BtsCI GGATG 2 cut(s) 520, 577
BtsIMutI CAGTG 2 cut(s) 255, 382
BveI ACCTGC 1 cut(s) 40
CciI TCATGA 1 cut(s) 307
Cfr10I RCCGGY 1 cut(s) 392
CsiI ACCWGGT 1 cut(s) 345
Csp6I GTAC 2 cut(s) 586, 648
CspAI ACCGGT 1 cut(s) 392
CviAII CATG 3 cut(s) 289, 308, 420
CviJI RGCY 9 cut(s) 153, 187, 211, 236, 432, 472, 481, 552, 573
CviKI_1 RGCY 9 cut(s) 153, 187, 211, 236, 432, 472, 481, 552, 573
CviQI GTAC 2 cut(s) 586, 648
DdeI CTNAG 2 cut(s) 439, 473
DpnI GATC 4 cut(s) 39, 92, 312, 363
DpnII GATC 4 cut(s) 37, 90, 310, 361
DraI TTTAAA 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 550
Ecl136II GAGCTC 1 cut(s) 432
Eco130I CCWWGG 1 cut(s) 581
Eco24I GRGCYC 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 432
EcoICRI GAGCTC 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 345, 500
EcoT14I CCWWGG 1 cut(s) 581
EcoT38I GRGCYC 1 cut(s) 434
ErhI CCWWGG 1 cut(s) 581
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 3 cut(s) 292, 311, 423
FaiI YATR 6 cut(s) 138, 290, 309, 421, 549, 593
FalI AAGNNNNNCTT 2 cut(s) 313, 345
FatI CATG 3 cut(s) 288, 307, 419
FauI CCCGC 2 cut(s) 85, 178
Fnu4HI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
FokI GGATG 2 cut(s) 527, 564
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
FspBI CTAG 2 cut(s) 582, 672
GluI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
HaeIII GGCC 1 cut(s) 552
HapII CCGG 1 cut(s) 393
Hin1II CATG 3 cut(s) 292, 311, 423
HinfI GANTC 1 cut(s) 486
HpaII CCGG 1 cut(s) 393
HphI GGTGA 3 cut(s) 212, 253, 472
Hpy166II GTNNAC 2 cut(s) 244, 650
Hpy188I TCNGA 4 cut(s) 231, 366, 442, 476
Hpy188III TCNNGA 2 cut(s) 308, 565
Hpy8I GTNNAC 2 cut(s) 244, 650
HpyAV CCTTC 5 cut(s) 222, 336, 361, 451, 584
HpyCH4III ACNGT 5 cut(s) 165, 180, 248, 259, 305
HpyCH4IV ACGT 1 cut(s) 588
HpyCH4V TGCA 2 cut(s) 115, 411
HpyF10VI GCNNNNNNNGC 8 cut(s) 86, 193, 199, 202, 205, 208, 411, 478
HpyF3I CTNAG 2 cut(s) 439, 473
HpySE526I ACGT 1 cut(s) 588
Hsp92II CATG 3 cut(s) 292, 311, 423
Kzo9I GATC 4 cut(s) 37, 90, 310, 361
LmnI GCTCC 1 cut(s) 429
LpnPI CCDG 9 cut(s) 35, 76, 332, 344, 359, 406, 487, 514, 566
Lsp1109I GCAGC 8 cut(s) 208, 211, 214, 217, 220, 223, 259, 423
LweI GCATC 1 cut(s) 261
MabI ACCWGGT 1 cut(s) 345
MaeI CTAG 2 cut(s) 582, 672
MaeII ACGT 1 cut(s) 588
MaeIII GTNAC 6 cut(s) 68, 180, 259, 299, 316, 530
MalI GATC 4 cut(s) 39, 92, 312, 363
MboI GATC 4 cut(s) 37, 90, 310, 361
MboII GAAGA 2 cut(s) 32, 207
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 434
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 6 cut(s) 31, 101, 415, 613, 657, 677
MluNI TGGCCA 1 cut(s) 552
MnlI CCTC 5 cut(s) 14, 84, 420, 456, 534
Mox20I TGGCCA 1 cut(s) 552
MscI TGGCCA 1 cut(s) 552
MseI TTAA 3 cut(s) 612, 629, 697
MslI CAYNNNNRTG 2 cut(s) 287, 306
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 2 cut(s) 236, 272
MspI CCGG 1 cut(s) 393
MspR9I CCNGG 2 cut(s) 347, 502
MvaI CCWGG 2 cut(s) 347, 502
MwoI GCNNNNNNNGC 8 cut(s) 86, 193, 199, 202, 205, 208, 411, 478
NdeII GATC 4 cut(s) 37, 90, 310, 361
NlaIII CATG 3 cut(s) 292, 311, 423
NlaIV GGNNCC 3 cut(s) 162, 363, 391
NmuCI GTSAC 3 cut(s) 180, 259, 299
PagI TCATGA 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PfeI GAWTC 1 cut(s) 486
PflMI CCANNNNNTGG 1 cut(s) 103
PinAI ACCGGT 1 cut(s) 392
PkrI GCNGC 9 cut(s) 195, 198, 201, 204, 207, 210, 238, 274, 413
Psp124BI GAGCTC 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 345, 500
PspGI CCWGG 2 cut(s) 345, 500
PspN4I GGNNCC 3 cut(s) 162, 363, 391
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 587, 649
RsaNI GTAC 2 cut(s) 586, 648
RseI CAYNNNNRTG 2 cut(s) 287, 306
SacI GAGCTC 1 cut(s) 434
SaqAI TTAA 3 cut(s) 612, 629, 697
SatI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
Sau3AI GATC 4 cut(s) 37, 90, 310, 361
ScrFI CCNGG 2 cut(s) 347, 502
SduI GDGCHC 1 cut(s) 434
SexAI ACCWGGT 1 cut(s) 345
SfaNI GCATC 1 cut(s) 261
SmiMI CAYNNNNRTG 2 cut(s) 287, 306
Sse9I AATT 6 cut(s) 31, 101, 415, 613, 657, 677
SsiI CCGC 5 cut(s) 78, 171, 191, 194, 270
SspMI CTAG 2 cut(s) 582, 672
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 2 cut(s) 345, 500
StyI CCWWGG 1 cut(s) 581
TaaI ACNGT 5 cut(s) 165, 180, 248, 259, 305
TaiI ACGT 1 cut(s) 591
TasI AATT 6 cut(s) 31, 101, 415, 613, 657, 677
TauI GCSGC 1 cut(s) 196
TfiI GAWTC 1 cut(s) 486
Tru1I TTAA 3 cut(s) 612, 629, 697
Tru9I TTAA 3 cut(s) 612, 629, 697
TscAI CASTG 2 cut(s) 262, 382
TseFI GTSAC 3 cut(s) 180, 259, 299
TseI GCWGC 8 cut(s) 196, 199, 202, 205, 208, 236, 272, 411
Tsp45I GTSAC 3 cut(s) 180, 259, 299
TspDTI ATGAA 5 cut(s) 32, 396, 408, 470, 566
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 262, 382
Van91I CCANNNNNTGG 1 cut(s) 103
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XmaJI CCTAGG 1 cut(s) 581
XspI CTAG 2 cut(s) 582, 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.