Rw1G017560
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0014211
Physical Location & Seq
Forward (+)
38747231 .. 38750074
2844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G017560.1

Sequence Viewer

Length: 747 bp
ATGGAAGGAGGTCATCACAGAGGGGTTCCAAATTACGATCTTCATCAGCAGGTTTCGTTTTCCAGCAGTACCCCTCCCGCAGAACAAGTGATCCACCACGAATTGGGGTTCGTGCAGTTTGAAAATCATCATCATCATAATCAAGTTTTGAGCTTCTTGGCACCGTCTCCCGCACAACAATCACAGCCACCGCCGCCGCAGCAGCAGCAGCCTTCTTCTCACCATCATCAGACAGCTGCCGTGAACAGTAGCATCACTGTCACCACCACCGCTGCGACAAACACCAACATGGGGTTTAGTCACAGTCATGATCTTGTTACAAGACCTTCTTGGAATAACAACGACCAGGTGGGAACGCTGGATCCGAAGGGTGTCAGTGATGAAAACGGAACCGGTAATGGTAGCGATTGCAGCAATTCATGGTGGAGGAGCTCAAACTCAGAGAAAAGCAAGATGAAGGTGAGGAGAAAGCTCAGAGAGCCAAGATTCTGTTTCCAAACCAGGAGTGATGTGGATGTGCTTGATGATGGTTACAAGTGGAGGAAATATGGCCAGAAAGTTGTCAAGAACAGCCTTCATCCTAGAAGTTACTACCGGTGTACTCACAGTAACTGTCGGGTGAAGAAGAGGGTTGAACGACTGTCTGAAGATTGTCGTATGGTGATAACAACCTATGAAGGTAGACACAACCATATCCCTTCAGATGACTCTAATTCATCTGAGCATGAATGCTTTACCTCTTTCTAA

Protein Analysis

248

Amino Acids

28.23

Weight (kDa)

8.23

Isoelectric Point (pI)

49.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 175 - 231 1.9e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0014211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44745
fragaria_vesca FvH4_7g10440 FvH4_7g10440 FvH4_7g10440
malus_domestica MD07G1110400.v1.1
prunus_persica Prupe.2G133800_v2.0.a1 Prupe.2G133800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348121
rosa_laevigata RLG00000028687
rosa_roxburghii Rroxscaffold_4G00307200
rosa_rugosa Rorug01G0192600
rosa_samantha Rh1AG210300 Rh1BG176000 Rh1CG194900 Rh1DG205800
rosa_wichuraiana Rw0G011400 Rw1G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 40
AccB1I GGYRCC 1 cut(s) 160
AccB7I CCANNNNNTGG 1 cut(s) 103
AccI GTMKAC 1 cut(s) 682
AciI CCGC 6 cut(s) 78, 171, 191, 194, 197, 270
AclWI GGATC 3 cut(s) 85, 356, 369
AcoI YGGCCR 1 cut(s) 550
AcuI CTGAAG 2 cut(s) 666, 684
AfaI GTAC 2 cut(s) 70, 601
AfiI CCNNNNNNNGG 2 cut(s) 103, 291
AgeI ACCGGT 2 cut(s) 392, 594
AgsI TTSAA 2 cut(s) 122, 635
AjnI CCWGG 2 cut(s) 345, 500
AloI GAACNNNNNNTCC 4 cut(s) 75, 107, 346, 378
AluBI AGCT 4 cut(s) 153, 236, 432, 472
AluI AGCT 4 cut(s) 153, 236, 432, 472
Alw21I GWGCWC 1 cut(s) 434
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 3 cut(s) 85, 356, 369
AlwNI CAGNNNCTG 1 cut(s) 612
AoxI GGCC 1 cut(s) 550
ApeKI GCWGC 7 cut(s) 199, 202, 205, 208, 236, 272, 411
AsiGI ACCGGT 2 cut(s) 392, 594
AsuHPI GGTGA 5 cut(s) 212, 253, 472, 631, 673
BalI TGGCCA 1 cut(s) 552
BamHI GGATCC 1 cut(s) 361
BanI GGYRCC 1 cut(s) 160
BanII GRGCYC 1 cut(s) 434
Bbv12I GWGCWC 1 cut(s) 434
BbvI GCAGC 7 cut(s) 211, 214, 217, 220, 223, 259, 423
BccI CCATC 2 cut(s) 231, 521
BceAI ACGGC 1 cut(s) 224
BciT130I CCWGG 2 cut(s) 347, 502
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 582
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
BlsI GCNGC 9 cut(s) 195, 198, 201, 204, 207, 210, 238, 274, 413
Bme1390I CCNGG 2 cut(s) 347, 502
BmiI GGNNCC 4 cut(s) 27, 162, 363, 391
BmrFI CCNGG 2 cut(s) 347, 502
BmsI GCATC 1 cut(s) 261
BsaBI GATNNNNATC 1 cut(s) 42
BsaWI WCCGGW 2 cut(s) 392, 594
Bsc4I CCNNNNNNNGG 2 cut(s) 103, 291
Bse118I RCCGGY 2 cut(s) 392, 594
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 347, 502
BseGI GGATG 2 cut(s) 520, 577
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 103, 291
BseMII CTCAG 3 cut(s) 453, 487, 711
BseRI GAGGAG 2 cut(s) 442, 478
BseXI GCAGC 7 cut(s) 211, 214, 217, 220, 223, 259, 423
BsgI GTGCAG 1 cut(s) 134
BshFI GGCC 1 cut(s) 552
BshNI GGYRCC 1 cut(s) 160
BshTI ACCGGT 2 cut(s) 392, 594
BsiHKAI GWGCWC 1 cut(s) 434
BsiSI CCGG 2 cut(s) 393, 595
BslI CCNNNNNNNGG 2 cut(s) 103, 291
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsmI GAATGC 1 cut(s) 734
BsnI GGCC 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 434
Bsp143I GATC 4 cut(s) 37, 90, 310, 361
BspACI CCGC 6 cut(s) 78, 171, 191, 194, 197, 270
BspANI GGCC 1 cut(s) 552
BspCNI CTCAG 3 cut(s) 452, 486, 712
BspHI TCATGA 1 cut(s) 307
BspLI GGNNCC 4 cut(s) 27, 162, 363, 391
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 3 cut(s) 85, 356, 369
BspT107I GGYRCC 1 cut(s) 160
BsrFI RCCGGY 2 cut(s) 392, 594
BssAI RCCGGY 2 cut(s) 392, 594
BssMI GATC 4 cut(s) 37, 90, 310, 361
Bst2UI CCWGG 2 cut(s) 347, 502
Bst4CI ACNGT 7 cut(s) 165, 248, 259, 305, 608, 614, 642
Bst6I CTCTTC 1 cut(s) 620
BstDEI CTNAG 3 cut(s) 439, 473, 720
BstF5I GGATG 2 cut(s) 520, 577
BstKTI GATC 4 cut(s) 40, 93, 313, 364
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 4 cut(s) 37, 90, 310, 361
BstMWI GCNNNNNNNGC 7 cut(s) 193, 199, 202, 205, 208, 411, 478
BstNI CCWGG 2 cut(s) 347, 502
BstSCI CCNGG 2 cut(s) 345, 500
BstV1I GCAGC 7 cut(s) 211, 214, 217, 220, 223, 259, 423
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 552
BtsCI GGATG 2 cut(s) 520, 577
BtsIMutI CAGTG 2 cut(s) 255, 382
BveI ACCTGC 1 cut(s) 40
CaiI CAGNNNCTG 1 cut(s) 612
CciI TCATGA 1 cut(s) 307
Cfr10I RCCGGY 2 cut(s) 392, 594
CsiI ACCWGGT 1 cut(s) 345
Csp6I GTAC 2 cut(s) 69, 600
CspAI ACCGGT 2 cut(s) 392, 594
CviAII CATG 4 cut(s) 289, 308, 420, 725
CviJI RGCY 9 cut(s) 153, 187, 211, 236, 432, 472, 481, 552, 573
CviKI_1 RGCY 9 cut(s) 153, 187, 211, 236, 432, 472, 481, 552, 573
CviQI GTAC 2 cut(s) 69, 600
DdeI CTNAG 3 cut(s) 439, 473, 720
DpnI GATC 4 cut(s) 39, 92, 312, 363
DpnII GATC 4 cut(s) 37, 90, 310, 361
EaeI YGGCCR 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 620
EarI CTCTTC 1 cut(s) 620
Ecl136II GAGCTC 1 cut(s) 432
Eco24I GRGCYC 1 cut(s) 434
Eco53kI GAGCTC 1 cut(s) 432
Eco57I CTGAAG 2 cut(s) 666, 684
EcoICRI GAGCTC 1 cut(s) 432
EcoRII CCWGG 2 cut(s) 345, 500
EcoT38I GRGCYC 1 cut(s) 434
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 4 cut(s) 292, 311, 423, 728
FaiI YATR 9 cut(s) 138, 290, 309, 421, 549, 659, 675, 693, 726
FalI AAGNNNNNCTT 2 cut(s) 313, 345
FatI CATG 4 cut(s) 288, 307, 419, 724
FauI CCCGC 2 cut(s) 85, 178
FblI GTMKAC 1 cut(s) 682
Fnu4HI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
FokI GGATG 2 cut(s) 527, 564
FriOI GRGCYC 1 cut(s) 434
Fsp4HI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
FspBI CTAG 1 cut(s) 582
GluI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
HaeIII GGCC 1 cut(s) 552
HapII CCGG 2 cut(s) 393, 595
Hin1II CATG 4 cut(s) 292, 311, 423, 728
HinfI GANTC 2 cut(s) 486, 707
HpaII CCGG 2 cut(s) 393, 595
HphI GGTGA 5 cut(s) 212, 253, 472, 631, 673
Hpy166II GTNNAC 3 cut(s) 244, 600, 683
Hpy188I TCNGA 7 cut(s) 231, 366, 442, 476, 646, 703, 721
Hpy188III TCNNGA 2 cut(s) 308, 565
Hpy8I GTNNAC 3 cut(s) 244, 600, 683
HpyAV CCTTC 7 cut(s) 222, 336, 361, 451, 584, 671, 708
HpyCH4III ACNGT 7 cut(s) 165, 248, 259, 305, 608, 614, 642
HpyCH4V TGCA 2 cut(s) 115, 411
HpyF10VI GCNNNNNNNGC 7 cut(s) 193, 199, 202, 205, 208, 411, 478
HpyF3I CTNAG 3 cut(s) 439, 473, 720
Hsp92II CATG 4 cut(s) 292, 311, 423, 728
Kzo9I GATC 4 cut(s) 37, 90, 310, 361
LmnI GCTCC 1 cut(s) 429
Lsp1109I GCAGC 7 cut(s) 211, 214, 217, 220, 223, 259, 423
LweI GCATC 1 cut(s) 261
MabI ACCWGGT 1 cut(s) 345
MaeI CTAG 1 cut(s) 582
MaeIII GTNAC 6 cut(s) 259, 299, 316, 530, 587, 608
MalI GATC 4 cut(s) 39, 92, 312, 363
MboI GATC 4 cut(s) 37, 90, 310, 361
MboII GAAGA 5 cut(s) 32, 207, 634, 637, 659
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 434
MlsI TGGCCA 1 cut(s) 552
MluCI AATT 4 cut(s) 31, 101, 415, 712
MluNI TGGCCA 1 cut(s) 552
MlyI GAGTC 1 cut(s) 701
MnlI CCTC 6 cut(s) 14, 84, 420, 456, 534, 621
Mox20I TGGCCA 1 cut(s) 552
MscI TGGCCA 1 cut(s) 552
MslI CAYNNNNRTG 2 cut(s) 287, 306
Msp20I TGGCCA 1 cut(s) 552
MspA1I CMGCKG 2 cut(s) 236, 272
MspI CCGG 2 cut(s) 393, 595
MspR9I CCNGG 2 cut(s) 347, 502
Mva1269I GAATGC 1 cut(s) 734
MvaI CCWGG 2 cut(s) 347, 502
MwoI GCNNNNNNNGC 7 cut(s) 193, 199, 202, 205, 208, 411, 478
NdeII GATC 4 cut(s) 37, 90, 310, 361
NlaIII CATG 4 cut(s) 292, 311, 423, 728
NlaIV GGNNCC 4 cut(s) 27, 162, 363, 391
NmuCI GTSAC 2 cut(s) 259, 299
PagI TCATGA 1 cut(s) 307
PcsI WCGNNNNNNNCGW 1 cut(s) 362
PctI GAATGC 1 cut(s) 734
PfeI GAWTC 1 cut(s) 486
PflMI CCANNNNNTGG 1 cut(s) 103
PinAI ACCGGT 2 cut(s) 392, 594
PkrI GCNGC 9 cut(s) 195, 198, 201, 204, 207, 210, 238, 274, 413
PleI GAGTC 1 cut(s) 701
PpsI GAGTC 1 cut(s) 701
Psp124BI GAGCTC 1 cut(s) 434
Psp6I CCWGG 2 cut(s) 345, 500
PspGI CCWGG 2 cut(s) 345, 500
PspN4I GGNNCC 4 cut(s) 27, 162, 363, 391
PstNI CAGNNNCTG 1 cut(s) 612
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 236
RsaI GTAC 2 cut(s) 70, 601
RsaNI GTAC 2 cut(s) 69, 600
RseI CAYNNNNRTG 2 cut(s) 287, 306
SacI GAGCTC 1 cut(s) 434
SatI GCNGC 9 cut(s) 194, 197, 200, 203, 206, 209, 237, 273, 412
Sau3AI GATC 4 cut(s) 37, 90, 310, 361
SchI GAGTC 1 cut(s) 701
ScrFI CCNGG 2 cut(s) 347, 502
SduI GDGCHC 1 cut(s) 434
SexAI ACCWGGT 1 cut(s) 345
SfaNI GCATC 1 cut(s) 261
SmiMI CAYNNNNRTG 2 cut(s) 287, 306
Sse9I AATT 4 cut(s) 31, 101, 415, 712
SsiI CCGC 6 cut(s) 78, 171, 191, 194, 197, 270
SspMI CTAG 1 cut(s) 582
SstI GAGCTC 1 cut(s) 434
StyD4I CCNGG 2 cut(s) 345, 500
TaaI ACNGT 7 cut(s) 165, 248, 259, 305, 608, 614, 642
TasI AATT 4 cut(s) 31, 101, 415, 712
TatI WGTACW 1 cut(s) 599
TauI GCSGC 2 cut(s) 196, 199
TfiI GAWTC 1 cut(s) 486
TscAI CASTG 2 cut(s) 262, 382
TseFI GTSAC 2 cut(s) 259, 299
TseI GCWGC 7 cut(s) 199, 202, 205, 208, 236, 272, 411
Tsp45I GTSAC 2 cut(s) 259, 299
TspDTI ATGAA 8 cut(s) 32, 396, 408, 470, 566, 690, 705, 741
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 262, 382
Van91I CCANNNNNTGG 1 cut(s) 103
XcmI CCANNNNNNNNNTGG 1 cut(s) 508
XmiI GTMKAC 1 cut(s) 682
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.