Rroxscaffold_4G00307200
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014211
Physical Location & Seq
Reverse (-)
28340868 .. 28344184
3317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00307200.1

Sequence Viewer

Length: 459 bp
ATGGGGTTTAGTCACAGTCATGATCTTGTTACAAGACCTTCTTGGAATAACAACGACCAGGTGGGAACGCTGGATCCGAAGGGTGTCAGTGATGAAAACGGAACCGGTAATGGTAGCGATTGCAGCAATTCATGGTGGAGGAGCTCAAACTCAGAGAAAAGCAAGATGAAGGTGAGGAGAAAGCTCAGAGAGCCAAGATTCTGTTTCCAAACCAGGAGTGATGTGGATGTGCTTGATGATGGTTACAAGTGGAGGAAATATGGCCAGAAAGTTGTCAAGAACAGCCTTCATCCTAGAAGTTACTACCGGTGTACTCACAATAACTGTCGGGTGAAGAAGAGGGTTGAACGACTGTCTGAAGATTGTCGTATGGTGATAACAACCTATGAAGGTAGACACAACCATATCCCTTCAGATGACTCTAATTCATCTGAGCATGAATGCTTTACCTCTTTCTAA

Protein Analysis

152

Amino Acids

17.75

Weight (kDa)

9.08

Isoelectric Point (pI)

41.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 79 - 135 7.6e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G44745
fragaria_vesca FvH4_7g10440 FvH4_7g10440 FvH4_7g10440
malus_domestica MD07G1110400.v1.1
prunus_persica Prupe.2G133800_v2.0.a1 Prupe.2G133800_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0348121
rosa_laevigata RLG00000028687
rosa_roxburghii Rroxscaffold_4G00307200
rosa_rugosa Rorug01G0192600
rosa_samantha Rh1AG210300 Rh1BG176000 Rh1CG194900 Rh1DG205800
rosa_wichuraiana Rw0G011400 Rw1G017560

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 394
AclWI GGATC 2 cut(s) 68, 81
AcoI YGGCCR 1 cut(s) 262
AcuI CTGAAG 2 cut(s) 378, 396
AfaI GTAC 1 cut(s) 313
AgeI ACCGGT 2 cut(s) 104, 306
AgsI TTSAA 1 cut(s) 347
AjnI CCWGG 2 cut(s) 57, 212
AloI GAACNNNNNNTCC 2 cut(s) 58, 90
AluBI AGCT 2 cut(s) 144, 184
AluI AGCT 2 cut(s) 144, 184
Alw21I GWGCWC 1 cut(s) 146
AlwI GGATC 2 cut(s) 68, 81
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 1 cut(s) 123
AsiGI ACCGGT 2 cut(s) 104, 306
AsuHPI GGTGA 3 cut(s) 184, 343, 385
BalI TGGCCA 1 cut(s) 264
BamHI GGATCC 1 cut(s) 73
BanII GRGCYC 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 146
BbvI GCAGC 1 cut(s) 135
BccI CCATC 1 cut(s) 233
BciT130I CCWGG 2 cut(s) 59, 214
BfaI CTAG 1 cut(s) 294
BisI GCNGC 1 cut(s) 124
BlsI GCNGC 1 cut(s) 125
Bme1390I CCNGG 2 cut(s) 59, 214
BmiI GGNNCC 2 cut(s) 75, 103
BmrFI CCNGG 2 cut(s) 59, 214
BsaWI WCCGGW 2 cut(s) 104, 306
Bse118I RCCGGY 2 cut(s) 104, 306
BseBI CCWGG 2 cut(s) 59, 214
BseGI GGATG 2 cut(s) 232, 289
BseMII CTCAG 3 cut(s) 165, 199, 423
BseRI GAGGAG 2 cut(s) 154, 190
BseXI GCAGC 1 cut(s) 135
BshFI GGCC 1 cut(s) 264
BshTI ACCGGT 2 cut(s) 104, 306
BsiHKAI GWGCWC 1 cut(s) 146
BsiSI CCGG 2 cut(s) 105, 307
BsmI GAATGC 1 cut(s) 446
BsnI GGCC 1 cut(s) 264
Bsp1286I GDGCHC 1 cut(s) 146
Bsp143I GATC 2 cut(s) 22, 73
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 3 cut(s) 164, 198, 424
BspHI TCATGA 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 75, 103
BspPI GGATC 2 cut(s) 68, 81
BsrFI RCCGGY 2 cut(s) 104, 306
BssAI RCCGGY 2 cut(s) 104, 306
BssMI GATC 2 cut(s) 22, 73
Bst2UI CCWGG 2 cut(s) 59, 214
Bst4CI ACNGT 3 cut(s) 17, 326, 354
Bst6I CTCTTC 1 cut(s) 332
BstDEI CTNAG 3 cut(s) 151, 185, 432
BstF5I GGATG 2 cut(s) 232, 289
BstKTI GATC 2 cut(s) 25, 76
BstMBI GATC 2 cut(s) 22, 73
BstMWI GCNNNNNNNGC 2 cut(s) 123, 190
BstNI CCWGG 2 cut(s) 59, 214
BstSCI CCNGG 2 cut(s) 57, 212
BstV1I GCAGC 1 cut(s) 135
BstX2I RGATCY 1 cut(s) 73
BstYI RGATCY 1 cut(s) 73
BsuRI GGCC 1 cut(s) 264
BtsCI GGATG 2 cut(s) 232, 289
BtsIMutI CAGTG 1 cut(s) 94
CciI TCATGA 1 cut(s) 19
Cfr10I RCCGGY 2 cut(s) 104, 306
CsiI ACCWGGT 1 cut(s) 57
Csp6I GTAC 1 cut(s) 312
CspAI ACCGGT 2 cut(s) 104, 306
CviAII CATG 3 cut(s) 20, 132, 437
CviJI RGCY 5 cut(s) 144, 184, 193, 264, 285
CviKI_1 RGCY 5 cut(s) 144, 184, 193, 264, 285
CviQI GTAC 1 cut(s) 312
DdeI CTNAG 3 cut(s) 151, 185, 432
DpnI GATC 2 cut(s) 24, 75
DpnII GATC 2 cut(s) 22, 73
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 332
EarI CTCTTC 1 cut(s) 332
Ecl136II GAGCTC 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 144
Eco57I CTGAAG 2 cut(s) 378, 396
EcoICRI GAGCTC 1 cut(s) 144
EcoRII CCWGG 2 cut(s) 57, 212
EcoT38I GRGCYC 1 cut(s) 146
FaeI CATG 3 cut(s) 23, 135, 440
FaiI YATR 7 cut(s) 21, 133, 261, 371, 387, 405, 438
FalI AAGNNNNNCTT 2 cut(s) 25, 57
FatI CATG 3 cut(s) 19, 131, 436
FblI GTMKAC 1 cut(s) 394
Fnu4HI GCNGC 1 cut(s) 124
FokI GGATG 2 cut(s) 239, 276
FriOI GRGCYC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 124
FspBI CTAG 1 cut(s) 294
GluI GCNGC 1 cut(s) 124
HaeIII GGCC 1 cut(s) 264
HapII CCGG 2 cut(s) 105, 307
Hin1II CATG 3 cut(s) 23, 135, 440
HinfI GANTC 2 cut(s) 198, 419
HpaII CCGG 2 cut(s) 105, 307
HphI GGTGA 3 cut(s) 184, 343, 385
Hpy166II GTNNAC 2 cut(s) 312, 395
Hpy188I TCNGA 6 cut(s) 78, 154, 188, 358, 415, 433
Hpy188III TCNNGA 2 cut(s) 20, 277
Hpy8I GTNNAC 2 cut(s) 312, 395
HpyAV CCTTC 6 cut(s) 48, 73, 163, 296, 383, 420
HpyCH4III ACNGT 3 cut(s) 17, 326, 354
HpyCH4V TGCA 1 cut(s) 123
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 190
HpyF3I CTNAG 3 cut(s) 151, 185, 432
Hsp92II CATG 3 cut(s) 23, 135, 440
Kzo9I GATC 2 cut(s) 22, 73
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 8 cut(s) 44, 56, 71, 118, 199, 226, 278, 320
Lsp1109I GCAGC 1 cut(s) 135
MabI ACCWGGT 1 cut(s) 57
MaeI CTAG 1 cut(s) 294
MaeIII GTNAC 4 cut(s) 11, 28, 242, 299
MalI GATC 2 cut(s) 24, 75
MboI GATC 2 cut(s) 22, 73
MboII GAAGA 3 cut(s) 346, 349, 371
MflI RGATCY 1 cut(s) 73
MhlI GDGCHC 1 cut(s) 146
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 2 cut(s) 127, 424
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 1 cut(s) 413
MnlI CCTC 4 cut(s) 132, 168, 246, 333
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 18
Msp20I TGGCCA 1 cut(s) 264
MspI CCGG 2 cut(s) 105, 307
MspR9I CCNGG 2 cut(s) 59, 214
Mva1269I GAATGC 1 cut(s) 446
MvaI CCWGG 2 cut(s) 59, 214
MwoI GCNNNNNNNGC 2 cut(s) 123, 190
NdeII GATC 2 cut(s) 22, 73
NlaIII CATG 3 cut(s) 23, 135, 440
NlaIV GGNNCC 2 cut(s) 75, 103
NmuCI GTSAC 1 cut(s) 11
PagI TCATGA 1 cut(s) 19
PcsI WCGNNNNNNNCGW 1 cut(s) 74
PctI GAATGC 1 cut(s) 446
PfeI GAWTC 1 cut(s) 198
PinAI ACCGGT 2 cut(s) 104, 306
PkrI GCNGC 1 cut(s) 125
PleI GAGTC 1 cut(s) 413
PpsI GAGTC 1 cut(s) 413
Psp124BI GAGCTC 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 57, 212
PspGI CCWGG 2 cut(s) 57, 212
PspN4I GGNNCC 2 cut(s) 75, 103
PsuI RGATCY 1 cut(s) 73
RsaI GTAC 1 cut(s) 313
RsaNI GTAC 1 cut(s) 312
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 146
SatI GCNGC 1 cut(s) 124
Sau3AI GATC 2 cut(s) 22, 73
SchI GAGTC 1 cut(s) 413
ScrFI CCNGG 2 cut(s) 59, 214
SduI GDGCHC 1 cut(s) 146
SetI ASST 8 cut(s) 40, 63, 146, 174, 186, 386, 394, 452
SexAI ACCWGGT 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 18
Sse9I AATT 2 cut(s) 127, 424
SspMI CTAG 1 cut(s) 294
SstI GAGCTC 1 cut(s) 146
StyD4I CCNGG 2 cut(s) 57, 212
TaaI ACNGT 3 cut(s) 17, 326, 354
TasI AATT 2 cut(s) 127, 424
TatI WGTACW 1 cut(s) 311
TfiI GAWTC 1 cut(s) 198
TscAI CASTG 1 cut(s) 94
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 1 cut(s) 123
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 7 cut(s) 108, 120, 182, 278, 402, 417, 453
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 1 cut(s) 94
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XmiI GTMKAC 1 cut(s) 394
XspI CTAG 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.