Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0001902

Physical Location & Seq

Genomic Coordinates Forward (+)

20867418 .. 20870624

Gene Structure 3207 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1143200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1671 bp

ATGAAGGAATTGAATACGGATCTATTCGACCCGAGAACTACGCTGATGGAACCCGACTTTTCGCGGGCCGTGACGCCGTTGGCCGACGCTGACGGCGACGATTTCGCCTTCGCCTTTAACGACAGTAACTTCTCCGACCGACAGCTTCGGATCGAGATCATGGGTGACACACCCGAGTCCAGACCCGATTCCGAGGCTTGCACCAGTATCGCTGATTGGGCCCTCCACCGGAAGCGGCGGAGAGAAGACATCAAGAAGGAAAACATTCTGGATCTCGCCGAATGCCCCGAGGAGCAAATCTTGAATGACAACCAGCCTGATATGGATGATTGCGAGGGCTGTGAAAATCAAGATGAGGAACCAGTTGCAATGGTTGAAGAGTCACCTTCAGGGGATGAAGCTGCAAACAGCAATGACTCAGACTGGGGAATGGATTGTTCTACAGTTGTGAGAGTTAAAACATTGCACATTAGCTCTCCAATTTTGGCAGCCAAGAGTCCTTTTTTCTATAAGCTCTTTTCAAATGGGATGAGGGAGTCTGAACAGCGACATGTAACCTTGCGAATCAGTGCGTCTGAAGAAGCTGCCCTCATGGAGCTCCTAAATTTTATGTACAGCAATACCTTATCCACCACCACAGCTCCTGCTCTACTGGACGTGTTGATGGCTGCTGACAAATTTGAGGTAGCTTCATGCATGAGGTATTGCAGCCGGCTACTGAGGAATATGCCCATGACACCTGAATCTGCGTTGCTTTATCTAGAGCTTCCTTCTAGTGTCTTAATGGCTGAAGCAGTCCAGGCGTTGACTGATGCTGCAAAGCAGTACCTCGCTGTCCGTTACAAGGACATAAGCAAGTTTTTTGAAGAGGTGATGGCCTTACCCCTAGCTGGAATAGAGGCAATTATATCCAGTGATGAACTCCAGGTTGCATCAGAGGATGCTGTATATGACTTTGTGTTGAAATGGTCTAGGACTCACTACCCTAAGTTGGAGGAAAGGCGAGAAATCTTGGGTGCTCGACTTGCACGCTACATTCGCTTCCCATACATGACTTGTCGAAAGCTGAAAAAGGTCTTAACCTGTAATGATTTTGACCACGAAGCTGCATCCAAGCTTGTCCTTGAGTCTCTTTTCTTCAAGGCCGAGGCTCCGCACAGGCAGCGAATTCTGGCAGCAGAAGAGTCTGCTACCTTGAATCGTCGGTTTGTGGAGCGGGCATACAAGTATCGCCCTGTTAAGGTGGTGGAATTTGATCTTCCCAGACAGCAGTGTGTGGTGTACCTGGACTTGAAGCGAGAGGAGTGTGCAAATTTATTCCCATCCGGACGGGTATATTCTCAGGCATTCCACTTGGGTGGGCAAGGGTTCTTCCTCTCTGCGCATTGCAACATGGACCAGCAGAGTTCTTTCCATTGTTTTGGGCTGTTTTTGGGGATGCAGGAAAAAGGGTCAGTGAGTTTTGCAGTGGACTACGAATTTGCAGCGAGATCAAAGCCGACTGAGGAATTCATTAGCAAATACAAAGGCAACTACACATTTACAGGGGGCAAGGCAGTCGGGTACCGTAACTTATTTGCCATACCATGGACATCATTCACGGCCGAGGATAGTCCTTTCTTCATAAATGGCGTCCTTCATCTTCGAGCGGAGCTTACCATCAAACACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0008150 GO:0009314 GO:0009416 GO:0009628 GO:0009639 GO:0010114 GO:0031461 GO:0031463 GO:0032991 GO:0042802 GO:0042803 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464 GO:0046982 GO:0046983 GO:0050896 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BACK BTB

Protein Analysis

557

Amino Acids

63.19

Weight (kDa)

5.05

Isoelectric Point (pI)

52.84

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BTB	PF00651	144 - 241	3.2e-14	BTB/POZ domain
BTB_KLHL33	PF21536	172 - 236	7e-14	Kelch-like protein 33-like, BTB/POZ domain
BACK	PF07707	266 - 359	1.8e-09	BTB And C-terminal Kelch

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001902)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G46260 AT3G61600 AT3G61600
fragaria_vesca	FvH4_5g03751 FvH4_5g03751 FvH4_5g03751 FvH4_5g03751 FvH4_5g03751 FvH4_7g15752 FvH4_7g15752
malus_domestica	MD01G1074300.v1.1 MD07G1143200.v1.1 MD14G1117700.v1.1
prunus_persica	Prupe.2G182300_v2.0.a1 Prupe.2G182300_v2.0.a1 Prupe.5G112100_v2.0.a1 Prupe.5G112100_v2.0.a1 Prupe.5G112100_v2.0.a1
pyrus_communis	pycom06g09390 pycom07g14190
rosa_chinensis	RchiOBHm_Chr1g0358521 RchiOBHm_Chr7g0195841
rosa_laevigata	RLG00000004083 RLG00000027955
rosa_multiflora	Rmu_co8444369.1_g000001 Rmu_sc0001609.1_g000004 Rmu_sc0004016.1_g000020
rosa_roxburghii	Rroxscaffold_3G00260050 Rroxscaffold_4G00297840
rosa_rugosa	Rorug01G0262200 Rorug01G0262300 Rorug07G0026800 Rorug07G0026900 Rorug07G0027000 Rorug07G0027000 Rorug07G0027100
rosa_samantha	Rh1AG276900 Rh1BG243300 Rh1CG260500 Rh1DG271600 Rh7AG154600 Rh7BG156400 Rh7CG160100 Rh7DG155600
rosa_wichuraiana	Rw1G024620 Rw7G013210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1383
Acc65I	GGTACC	1 cut(s)	1563
AccB1I	GGYRCC	1 cut(s)	1563
AccB7I	CCANNNNNTGG	1 cut(s)	1587
AccBSI	CCGCTC	2 cut(s)	1216, 1649
AccII	CGCG	1 cut(s)	64
AccIII	TCCGGA	1 cut(s)	1325
AciI	CCGC	6 cut(s)	64, 235, 238, 1154, 1216, 1649
AclWI	GGATC	3 cut(s)	27, 158, 279
AcoI	YGGCCR	2 cut(s)	81, 1602
AcsI	RAATTY	7 cut(s)	604, 677, 1167, 1250, 1312, 1478, 1508
AcuI	CTGAAG	3 cut(s)	372, 597, 810
AcyI	GRCGYC	2 cut(s)	74, 1632
AfaI	GTAC	4 cut(s)	614, 827, 1283, 1565
AfiI	CCNNNNNNNGG	6 cut(s)	228, 844, 890, 991, 1240, 1587
AflIII	ACRYGT	2 cut(s)	550, 657
AgsI	TTSAA	9 cut(s)	13, 304, 377, 522, 866, 964, 1141, 1198, 1294
AjiI	CACGTC	1 cut(s)	658
AjnI	CCWGG	3 cut(s)	798, 924, 1284
AleI	CACNNNNGTG	1 cut(s)	1356
Alw21I	GWGCWC	2 cut(s)	600, 1021
Alw26I	GTCTC	1 cut(s)	1134
AlwI	GGATC	3 cut(s)	27, 158, 279
AlwNI	CAGNNNCTG	1 cut(s)	644
Ama87I	CYCGRG	3 cut(s)	31, 173, 287
Aor13HI	TCCGGA	1 cut(s)	1325
AoxI	GGCC	6 cut(s)	66, 81, 219, 876, 1143, 1602
ApaI	GGGCCC	1 cut(s)	223
ApoI	RAATTY	7 cut(s)	604, 677, 1167, 1250, 1312, 1478, 1508
ArsI	GACNNNNNNTTYG	2 cut(s)	1463, 1495
Asp700I	GAANNNNTTC	1 cut(s)	264
Asp718I	GGTACC	1 cut(s)	1563
AspLEI	GCGC	1 cut(s)	1384
AspS9I	GGNCC	4 cut(s)	66, 219, 220, 1396
AsuHPI	GGTGA	3 cut(s)	176, 375, 883
AvaI	CYCGRG	3 cut(s)	31, 173, 287
AvaII	GGWCC	1 cut(s)	1396
BaeGI	GKGCMC	1 cut(s)	223
BanI	GGYRCC	1 cut(s)	1563
BanII	GRGCYC	2 cut(s)	223, 600
BbsI	GAAGAC	1 cut(s)	252
Bbv12I	GWGCWC	2 cut(s)	600, 1021
BccI	CCATC	5 cut(s)	40, 658, 868, 1330, 1667
BceAI	ACGGC	4 cut(s)	53, 61, 109, 1617
BcgI	CGANNNNNNTGC	4 cut(s)	190, 224, 1540, 1574
BciT130I	CCWGG	3 cut(s)	800, 926, 1286
BcoDI	GTCTC	1 cut(s)	1134
BfaI	CTAG	4 cut(s)	761, 774, 887, 972
BfmI	CTRYAG	1 cut(s)	441
Bme1390I	CCNGG	3 cut(s)	800, 926, 1286
Bme18I	GGWCC	1 cut(s)	1396
BmeT110I	CYCGRG	3 cut(s)	31, 173, 287
BmgBI	CACGTC	1 cut(s)	658
BmgT120I	GGNCC	4 cut(s)	66, 219, 220, 1396
BmiI	GGNNCC	5 cut(s)	51, 221, 360, 1152, 1565
BmrFI	CCNGG	3 cut(s)	800, 926, 1286
BmrI	ACTGGG	1 cut(s)	433
BmsI	GCATC	5 cut(s)	802, 931, 941, 1118, 1428
BmuI	ACTGGG	1 cut(s)	433
BpiI	GAAGAC	1 cut(s)	252
BpmI	CTGGAG	1 cut(s)	908
BpuEI	CTTGAG	1 cut(s)	1145
BsaBI	GATNNNNATC	1 cut(s)	155
BsaHI	GRCGYC	2 cut(s)	74, 1632
BsaJI	CCNNGG	5 cut(s)	192, 288, 1146, 1586, 1605
BsaWI	WCCGGW	2 cut(s)	228, 1325
BsaXI	ACNNNNNCTCC	2 cut(s)	625, 655
Bsc4I	CCNNNNNNNGG	6 cut(s)	228, 844, 890, 991, 1240, 1587
Bse118I	RCCGGY	1 cut(s)	711
Bse1I	ACTGG	5 cut(s)	204, 362, 428, 657, 912
Bse3DI	GCAATG	4 cut(s)	375, 418, 461, 1384
Bse8I	GATNNNNATC	1 cut(s)	155
BseAI	TCCGGA	1 cut(s)	1325
BseBI	CCWGG	3 cut(s)	800, 926, 1286
BseDI	CCNNGG	5 cut(s)	192, 288, 1146, 1586, 1605
BseGI	GGATG	7 cut(s)	331, 400, 534, 946, 1109, 1322, 1443
BseJI	GATNNNNATC	1 cut(s)	155
BseLI	CCNNNNNNNGG	6 cut(s)	228, 844, 890, 991, 1240, 1587
BseMI	GCAATG	4 cut(s)	375, 418, 461, 1384
BseMII	CTCAG	4 cut(s)	432, 710, 1355, 1494
BseNI	ACTGG	5 cut(s)	204, 362, 428, 657, 912
BseRI	GAGGAG	2 cut(s)	305, 1316
BseSI	GKGCMC	1 cut(s)	223
BseX3I	CGGCCG	1 cut(s)	1602
Bsh1236I	CGCG	1 cut(s)	64
Bsh1285I	CGRYCG	2 cut(s)	139, 1605
BshFI	GGCC	6 cut(s)	68, 83, 221, 878, 1145, 1604
BshNI	GGYRCC	1 cut(s)	1563
BsiEI	CGRYCG	2 cut(s)	139, 1605
BsiHKAI	GWGCWC	2 cut(s)	600, 1021
BsiHKCI	CYCGRG	3 cut(s)	31, 173, 287
BsiSI	CCGG	3 cut(s)	229, 712, 1326
BslI	CCNNNNNNNGG	6 cut(s)	228, 844, 890, 991, 1240, 1587
BsmAI	GTCTC	1 cut(s)	1134
BsmI	GAATGC	2 cut(s)	287, 1346
BsnI	GGCC	6 cut(s)	68, 83, 221, 878, 1145, 1604
BsoBI	CYCGRG	3 cut(s)	31, 173, 287
Bsp120I	GGGCCC	1 cut(s)	219
Bsp1286I	GDGCHC	3 cut(s)	223, 600, 1021
Bsp13I	TCCGGA	1 cut(s)	1325
Bsp1407I	TGTACA	1 cut(s)	612
Bsp143I	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
Bsp19I	CCATGG	1 cut(s)	1586
BspACI	CCGC	6 cut(s)	64, 235, 238, 1154, 1216, 1649
BspANI	GGCC	6 cut(s)	68, 83, 221, 878, 1145, 1604
BspCNI	CTCAG	4 cut(s)	431, 711, 1354, 1495
BspEI	TCCGGA	1 cut(s)	1325
BspFNI	CGCG	1 cut(s)	64
BspLI	GGNNCC	5 cut(s)	51, 221, 360, 1152, 1565
BspPI	GGATC	3 cut(s)	27, 158, 279
BspT107I	GGYRCC	1 cut(s)	1563
BsrBI	CCGCTC	2 cut(s)	1216, 1649
BsrDI	GCAATG	4 cut(s)	375, 418, 461, 1384
BsrFI	RCCGGY	1 cut(s)	711
BsrGI	TGTACA	1 cut(s)	612
BsrI	ACTGG	5 cut(s)	204, 362, 428, 657, 912
BssAI	RCCGGY	1 cut(s)	711
BssECI	CCNNGG	5 cut(s)	192, 288, 1146, 1586, 1605
BssMI	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
BssNI	GRCGYC	2 cut(s)	74, 1632
BssT1I	CCWWGG	1 cut(s)	1586
Bst2UI	CCWGG	3 cut(s)	800, 926, 1286
Bst4CI	ACNGT	3 cut(s)	125, 445, 1568
Bst6I	CTCTTC	3 cut(s)	372, 861, 1176
BstACI	GRCGYC	2 cut(s)	74, 1632
BstAUI	TGTACA	1 cut(s)	612
BstC8I	GCNNGC	5 cut(s)	66, 199, 713, 1030, 1218
BstDEI	CTNAG	5 cut(s)	418, 719, 987, 1341, 1503
BstDSI	CCRYGG	1 cut(s)	1586
BstF5I	GGATG	7 cut(s)	331, 400, 534, 946, 1109, 1322, 1443
BstFNI	CGCG	1 cut(s)	64
BstHHI	GCGC	1 cut(s)	1384
BstKTI	GATC	6 cut(s)	22, 153, 159, 274, 1258, 1493
BstMAI	GTCTC	1 cut(s)	1134
BstMBI	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
BstMCI	CGRYCG	2 cut(s)	139, 1605
BstMWI	GCNNNNNNNGC	5 cut(s)	218, 800, 1025, 1038, 1162
BstNI	CCWGG	3 cut(s)	800, 926, 1286
BstNSI	RCATGY	1 cut(s)	554
BstSCI	CCNGG	3 cut(s)	798, 924, 1284
BstSFI	CTRYAG	1 cut(s)	441
BstSLI	GKGCMC	1 cut(s)	223
BstUI	CGCG	1 cut(s)	64
BstV2I	GAAGAC	1 cut(s)	252
BstX2I	RGATCY	2 cut(s)	19, 271
BstXI	CCANNNNNNTGG	2 cut(s)	1358, 1421
BstYI	RGATCY	2 cut(s)	19, 271
BstZI	CGGCCG	1 cut(s)	1602
BsuRI	GGCC	6 cut(s)	68, 83, 221, 878, 1145, 1604
BtgI	CCRYGG	1 cut(s)	1586
BtrI	CACGTC	1 cut(s)	658
BtsCI	GGATG	7 cut(s)	331, 400, 534, 946, 1109, 1322, 1443
BtsI	GCAGTG	2 cut(s)	1277, 1473
BtsIMutI	CAGTG	6 cut(s)	574, 919, 1277, 1461, 1473, 1666
Cac8I	GCNNGC	5 cut(s)	66, 199, 713, 1030, 1218
CaiI	CAGNNNCTG	1 cut(s)	644
CfoI	GCGC	1 cut(s)	1384
Cfr10I	RCCGGY	1 cut(s)	711
Cfr13I	GGNCC	4 cut(s)	66, 219, 220, 1396
CseI	GACGC	4 cut(s)	82, 95, 561, 1621
Csp6I	GTAC	4 cut(s)	613, 826, 1282, 1564
CviAII	CATG	9 cut(s)	160, 551, 592, 693, 697, 733, 1051, 1393, 1587
CviQI	GTAC	4 cut(s)	613, 826, 1282, 1564
DdeI	CTNAG	5 cut(s)	418, 719, 987, 1341, 1503
DpnI	GATC	6 cut(s)	21, 152, 158, 273, 1257, 1492
DpnII	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
EaeI	YGGCCR	2 cut(s)	81, 1602
EagI	CGGCCG	1 cut(s)	1602
Eam1104I	CTCTTC	3 cut(s)	372, 861, 1176
EarI	CTCTTC	3 cut(s)	372, 861, 1176
EciI	GGCGGA	1 cut(s)	253
Ecl136II	GAGCTC	1 cut(s)	598
EclXI	CGGCCG	1 cut(s)	1602
Eco130I	CCWWGG	1 cut(s)	1586
Eco24I	GRGCYC	2 cut(s)	223, 600
Eco47I	GGWCC	1 cut(s)	1396
Eco52I	CGGCCG	1 cut(s)	1602
Eco53kI	GAGCTC	1 cut(s)	598
Eco57I	CTGAAG	3 cut(s)	372, 597, 810
Eco88I	CYCGRG	3 cut(s)	31, 173, 287
EcoICRI	GAGCTC	1 cut(s)	598
EcoO109I	RGGNCCY	1 cut(s)	220
EcoRI	GAATTC	2 cut(s)	1167, 1508
EcoRII	CCWGG	3 cut(s)	798, 924, 1284
EcoT14I	CCWWGG	1 cut(s)	1586
EcoT22I	ATGCAT	1 cut(s)	698
EcoT38I	GRGCYC	2 cut(s)	223, 600
ErhI	CCWWGG	1 cut(s)	1586
FaeI	CATG	9 cut(s)	163, 554, 595, 696, 700, 736, 1054, 1396, 1590
FatI	CATG	9 cut(s)	159, 550, 591, 692, 696, 732, 1050, 1392, 1586
FauI	CCCGC	2 cut(s)	57, 1209
FokI	GGATG	7 cut(s)	338, 407, 541, 953, 1096, 1309, 1450
FriOI	GRGCYC	2 cut(s)	223, 600
FspBI	CTAG	4 cut(s)	761, 774, 887, 972
FspI	TGCGCA	1 cut(s)	1383
GlaI	GCGC	1 cut(s)	1383
GsuI	CTGGAG	1 cut(s)	908
HaeIII	GGCC	6 cut(s)	68, 83, 221, 878, 1145, 1604
HapII	CCGG	3 cut(s)	229, 712, 1326
HgaI	GACGC	4 cut(s)	82, 95, 561, 1621
HhaI	GCGC	1 cut(s)	1384
Hin1I	GRCGYC	2 cut(s)	74, 1632
Hin1II	CATG	9 cut(s)	163, 554, 595, 696, 700, 736, 1054, 1396, 1590
Hin6I	GCGC	1 cut(s)	1382
HinP1I	GCGC	1 cut(s)	1382
HincII	GTYRAC	1 cut(s)	807
HindII	GTYRAC	1 cut(s)	807
HindIII	AAGCTT	1 cut(s)	1115
HpaII	CCGG	3 cut(s)	229, 712, 1326
HphI	GGTGA	3 cut(s)	176, 375, 883
Hpy166II	GTNNAC	3 cut(s)	807, 1282, 1471
Hpy188I	TCNGA	7 cut(s)	136, 150, 193, 421, 541, 577, 937
Hpy188III	TCNNGA	8 cut(s)	154, 180, 253, 269, 301, 350, 761, 1326
Hpy8I	GTNNAC	3 cut(s)	807, 1282, 1471
Hpy99I	CGWCG	3 cut(s)	89, 101, 1206
HpyAV	CCTTC	5 cut(s)	118, 250, 396, 780, 1646
HpyCH4III	ACNGT	3 cut(s)	125, 445, 1568
HpyCH4IV	ACGT	1 cut(s)	657
HpyF10VI	GCNNNNNNNGC	5 cut(s)	218, 800, 1025, 1038, 1162
HpyF3I	CTNAG	5 cut(s)	418, 719, 987, 1341, 1503
HpySE526I	ACGT	1 cut(s)	657
Hsp92I	GRCGYC	2 cut(s)	74, 1632
Hsp92II	CATG	9 cut(s)	163, 554, 595, 696, 700, 736, 1054, 1396, 1590
HspAI	GCGC	1 cut(s)	1382
Kpn2I	TCCGGA	1 cut(s)	1325
KpnI	GGTACC	1 cut(s)	1567
KroI	GCCGGC	1 cut(s)	711
KroNI	GCCGGC	1 cut(s)	713
Kzo9I	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
LmnI	GCTCC	7 cut(s)	292, 595, 603, 646, 1156, 1213, 1651
LweI	GCATC	5 cut(s)	802, 931, 941, 1118, 1428
MaeI	CTAG	4 cut(s)	761, 774, 887, 972
MaeII	ACGT	1 cut(s)	657
MaeIII	GTNAC	7 cut(s)	70, 125, 164, 381, 553, 839, 1568
MalI	GATC	6 cut(s)	21, 152, 158, 273, 1257, 1492
MbiI	CCGCTC	2 cut(s)	1216, 1649
MboI	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
MflI	RGATCY	2 cut(s)	19, 271
MhlI	GDGCHC	3 cut(s)	223, 600, 1021
MlyI	GAGTC	8 cut(s)	185, 389, 410, 505, 545, 970, 1136, 1193
MmeI	TCCRAC	2 cut(s)	159, 972
Mph1103I	ATGCAT	1 cut(s)	698
MroI	TCCGGA	1 cut(s)	1325
MroNI	GCCGGC	1 cut(s)	711
MroXI	GAANNNNTTC	1 cut(s)	264
MseI	TTAA	5 cut(s)	117, 456, 782, 1079, 1239
MslI	CAYNNNNRTG	1 cut(s)	1356
MspI	CCGG	3 cut(s)	229, 712, 1326
MspR9I	CCNGG	3 cut(s)	800, 926, 1286
Mva1269I	GAATGC	2 cut(s)	287, 1346
MvaI	CCWGG	3 cut(s)	800, 926, 1286
MvnI	CGCG	1 cut(s)	64
MwoI	GCNNNNNNNGC	5 cut(s)	218, 800, 1025, 1038, 1162
NaeI	GCCGGC	1 cut(s)	713
NcoI	CCATGG	1 cut(s)	1586
NdeII	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
NgoMIV	GCCGGC	1 cut(s)	711
NlaIII	CATG	9 cut(s)	163, 554, 595, 696, 700, 736, 1054, 1396, 1590
NlaIV	GGNNCC	5 cut(s)	51, 221, 360, 1152, 1565
NmeAIII	GCCGAG	2 cut(s)	1171, 1630
NmuCI	GTSAC	3 cut(s)	70, 164, 381
NsbI	TGCGCA	1 cut(s)	1383
NsiI	ATGCAT	1 cut(s)	698
NspI	RCATGY	1 cut(s)	554
OliI	CACNNNNGTG	1 cut(s)	1356
PciI	ACATGT	1 cut(s)	550
PcsI	WCGNNNNNNNCGW	2 cut(s)	93, 117
PctI	GAATGC	2 cut(s)	287, 1346
PdiI	GCCGGC	1 cut(s)	713
PdmI	GAANNNNTTC	1 cut(s)	264
PfeI	GAWTC	4 cut(s)	188, 564, 743, 1198
PflMI	CCANNNNNTGG	1 cut(s)	1587
PleI	GAGTC	8 cut(s)	184, 388, 410, 504, 544, 970, 1135, 1192
PpsI	GAGTC	8 cut(s)	184, 388, 410, 504, 544, 970, 1135, 1192
PscI	ACATGT	1 cut(s)	550
Psp124BI	GAGCTC	1 cut(s)	600
Psp6I	CCWGG	3 cut(s)	798, 924, 1284
PspGI	CCWGG	3 cut(s)	798, 924, 1284
PspN4I	GGNNCC	5 cut(s)	51, 221, 360, 1152, 1565
PspOMI	GGGCCC	1 cut(s)	219
PspPI	GGNCC	4 cut(s)	66, 219, 220, 1396
PstNI	CAGNNNCTG	1 cut(s)	644
PsuI	RGATCY	2 cut(s)	19, 271
RsaI	GTAC	4 cut(s)	614, 827, 1283, 1565
RsaNI	GTAC	4 cut(s)	613, 826, 1282, 1564
RseI	CAYNNNNRTG	1 cut(s)	1356
SacI	GAGCTC	1 cut(s)	600
SaqAI	TTAA	5 cut(s)	117, 456, 782, 1079, 1239
Sau3AI	GATC	6 cut(s)	19, 150, 156, 271, 1255, 1490
Sau96I	GGNCC	4 cut(s)	66, 219, 220, 1396
SchI	GAGTC	8 cut(s)	185, 389, 410, 505, 545, 970, 1136, 1193
ScrFI	CCNGG	3 cut(s)	800, 926, 1286
SduI	GDGCHC	3 cut(s)	223, 600, 1021
SfaNI	GCATC	5 cut(s)	802, 931, 941, 1118, 1428
SfcI	CTRYAG	1 cut(s)	441
SinI	GGWCC	1 cut(s)	1396
SmiMI	CAYNNNNRTG	1 cut(s)	1356
SmlI	CTYRAG	1 cut(s)	1124
SmoI	CTYRAG	1 cut(s)	1124
SsiI	CCGC	6 cut(s)	64, 235, 238, 1154, 1216, 1649
SspMI	CTAG	4 cut(s)	761, 774, 887, 972
SstI	GAGCTC	1 cut(s)	600
StyD4I	CCNGG	3 cut(s)	798, 924, 1284
StyI	CCWWGG	1 cut(s)	1586
TaaI	ACNGT	3 cut(s)	125, 445, 1568
TaiI	ACGT	1 cut(s)	660
TaqI	TCGA	5 cut(s)	27, 153, 1021, 1060, 1645
TaqII	GACCGA	1 cut(s)	153
TatI	WGTACW	1 cut(s)	612
TauI	GCSGC	1 cut(s)	238
TfiI	GAWTC	4 cut(s)	188, 564, 743, 1198
Tru1I	TTAA	5 cut(s)	117, 456, 782, 1079, 1239
Tru9I	TTAA	5 cut(s)	117, 456, 782, 1079, 1239
TscAI	CASTG	5 cut(s)	574, 919, 1277, 1461, 1473
TseFI	GTSAC	3 cut(s)	70, 164, 381
Tsp45I	GTSAC	3 cut(s)	70, 164, 381
TspDTI	ATGAA	7 cut(s)	17, 411, 681, 933, 1501, 1612, 1628
TspGWI	ACGGA	2 cut(s)	32, 827
TspRI	CASTG	5 cut(s)	574, 919, 1277, 1461, 1473
Van91I	CCANNNNNTGG	1 cut(s)	1587
VpaK11BI	GGWCC	1 cut(s)	1396
XapI	RAATTY	7 cut(s)	604, 677, 1167, 1250, 1312, 1478, 1508
XbaI	TCTAGA	1 cut(s)	760
XceI	RCATGY	1 cut(s)	554
XmnI	GAANNNNTTC	1 cut(s)	264
XspI	CTAG	4 cut(s)	761, 774, 887, 972
Zsp2I	ATGCAT	1 cut(s)	698

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.