Rorug07G0027000

BTB POZ domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0001902
Physical Location & Seq
Forward (+)
1908569 .. 1912628
4060 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0027000.1

Sequence Viewer

Length: 1248 bp
ATGGGGAGCTCGGAGGAGAAAGTTGTTGCTGTGATCATGGTGGGTGGGCCCACTAAAGGCACTAGATTCCGGCCACTGTCATTGAATATTCCGAAGCCACTTTTTCCTTTAGCGGGACAACCAATGGTTCATCATCCAATTTCTGCTTGTAAAAAGATTCCTAACCTGGGGCAAATCTTTCTCATTGGTTTCTATGAGGAGCGTGAGTTATCATTATATGTATCCGCAATCTCTAATGAGCTTAAGGTCCCCGTTAGATATTTGAGGGAAGACAGGCCACATGGTTCAGCTGGTGGACTTTATAACTTCAGAGATCTGATCATGGAAGACAGCCCGTCGCACATTTTCTTGCTGAACTGTGATGTTTGCTGCAGTTTTCCGCTTCCAGAAATGCTTGAGGCTCACAGAAAATATGGTGGGATGGGAACGATCCTGGTAATCAAGGTTTCTGCTGAGTCAGCAAGTCAGTTTGGGGAACTTGTAGCTGATCCAGTCACCAATGAACTGCTGCATTACACAGAGAAGCCTGAGACTTTTGTCAGTGACCGGATAAATTGTGGTGTCTACATATTCACACCGGATATTTTTAATGCCATAGAGGGTGTTTCCTCTCAGCGGAAGGACGGAGCTGACTTAAGACGTCTATCCAGTTTTGAAGCCCTGCACTCCGCAACAAGGAGTGCATCCACACATTTTGTAAGGTTAGATCAAGATATCCTATCCCCTCTTGCAGGAAAGAAGCAGTTATATACATATGAAACCATGGACTTCTGGGAACAGATTAAAACTCCTGGAATGTCCTTGAAATGTTCTGGGTTGTATCTTTCACAATTCAGAGTCACCAGTCCCCATCTGTTGGTGAGTGGGGATGGTACAAAGAGTGCCAGTATTTCTGGTGATGTTTACATCCATCCATCAGCAAAAGTACACCAAAGTGCTAAAATTGGCCCCAATGTCTCAATATCTGCAAATGCTCGTATAGGAGCTGGTGTGAGGCTCATAAGTTGTATCATACTTGACAATGTCGAAATTAAGGAAAATGCAGTTGTTATGCATGCAATTATTGGATGGAAATCTTCTATTGGGAAATGGTCTCGTATCCAGGCTAGTGGGAATTACAATTCTAAGCTCGGGATCACAATCCTTGGTGAAGATGTGACAGTTGAAGATGAAGTCGTGGTTATCAACAGCATTGTCCTCCCCCATAAGACACTCAATCTGAGTGTTCAGGAGGAAATAATTCTTTGA

Protein Analysis

415

Amino Acids

45.55

Weight (kDa)

6.51

Isoelectric Point (pI)

39.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NTP_transferase PF00483 10 - 205 3.1e-31 Nucleotidyl transferase
LbH_EIF2B PF25084 293 - 369 6.2e-09 EIF2B subunit epsilon LbH domain
Hexapep PF00132 295 - 328 7.9e-06 Bacterial transferase hexapeptide (six repeats)
GMPPB_C PF25087 297 - 414 1.3e-38 GMPPB C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 303
AatII GACGTC 1 cut(s) 643
AccB7I CCANNNNNTGG 1 cut(s) 856
AccI GTMKAC 1 cut(s) 564
AciI CCGC 5 cut(s) 113, 225, 380, 616, 669
AclWI GGATC 3 cut(s) 424, 482, 1142
AcoI YGGCCR 1 cut(s) 71
AcuI CTGAAG 1 cut(s) 292
AcyI GRCGYC 1 cut(s) 640
AfaI GTAC 2 cut(s) 874, 927
AfiI CCNNNNNNNGG 7 cut(s) 56, 113, 167, 615, 675, 731, 856
AflII CTTAAG 2 cut(s) 242, 634
AgsI TTSAA 4 cut(s) 85, 656, 805, 1166
AhdI GACNNNNNGTC 1 cut(s) 334
AjnI CCWGG 4 cut(s) 165, 432, 790, 1101
AleI CACNNNNGTG 1 cut(s) 933
AluBI AGCT 7 cut(s) 9, 241, 290, 485, 629, 986, 1129
AluI AGCT 7 cut(s) 9, 241, 290, 485, 629, 986, 1129
Alw21I GWGCWC 1 cut(s) 11
Alw26I GTCTC 3 cut(s) 524, 961, 1098
AlwI GGATC 3 cut(s) 424, 482, 1142
Ama87I CYCGRG 1 cut(s) 1130
AoxI GGCC 4 cut(s) 47, 71, 275, 946
ApaI GGGCCC 1 cut(s) 51
ApeKI GCWGC 2 cut(s) 369, 508
Asp700I GAANNNNTTC 1 cut(s) 1239
AspS9I GGNCC 4 cut(s) 47, 48, 247, 947
AsuHPI GGTGA 5 cut(s) 487, 832, 871, 908, 1160
AvaI CYCGRG 1 cut(s) 1130
AvaII GGWCC 1 cut(s) 247
BaeGI GKGCMC 1 cut(s) 51
BanII GRGCYC 2 cut(s) 11, 51
BbsI GAAGAC 2 cut(s) 276, 333
Bbv12I GWGCWC 1 cut(s) 11
BbvI GCAGC 2 cut(s) 356, 495
BccI CCATC 6 cut(s) 415, 858, 863, 918, 922, 1062
BciT130I CCWGG 4 cut(s) 167, 434, 792, 1103
BciVI GTATCC 2 cut(s) 232, 1109
BclI TGATCA 2 cut(s) 33, 318
BcoDI GTCTC 3 cut(s) 524, 961, 1098
BfaI CTAG 2 cut(s) 63, 1107
BfmI CTRYAG 1 cut(s) 370
BfrI CTTAAG 2 cut(s) 242, 634
BfuI GTATCC 2 cut(s) 232, 1109
BglII AGATCT 1 cut(s) 313
BisI GCNGC 2 cut(s) 370, 509
BlsI GCNGC 2 cut(s) 371, 510
Bme1390I CCNGG 4 cut(s) 167, 434, 792, 1103
Bme18I GGWCC 1 cut(s) 247
BmeRI GACNNNNNGTC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 1130
BmgT120I GGNCC 4 cut(s) 47, 48, 247, 947
BmiI GGNNCC 3 cut(s) 49, 249, 949
BmrFI CCNGG 4 cut(s) 167, 434, 792, 1103
BmsI GCATC 1 cut(s) 692
BoxI GACNNNNGTC 1 cut(s) 536
BpiI GAAGAC 2 cut(s) 276, 333
BpuEI CTTGAG 1 cut(s) 416
BsaBI GATNNNNATC 2 cut(s) 1072, 1139
BsaHI GRCGYC 1 cut(s) 640
BsaI GGTCTC 1 cut(s) 1098
BsaJI CCNNGG 3 cut(s) 166, 762, 1144
BsaWI WCCGGW 2 cut(s) 546, 577
BsaXI ACNNNNNCTCC 4 cut(s) 191, 221, 975, 1005
Bsc4I CCNNNNNNNGG 7 cut(s) 56, 113, 167, 615, 675, 731, 856
Bse1I ACTGG 4 cut(s) 491, 648, 843, 885
Bse8I GATNNNNATC 2 cut(s) 1072, 1139
BseBI CCWGG 4 cut(s) 167, 434, 792, 1103
BseDI CCNNGG 3 cut(s) 166, 762, 1144
BseGI GGATG 7 cut(s) 133, 426, 683, 874, 906, 910, 1073
BseJI GATNNNNATC 2 cut(s) 1072, 1139
BseLI CCNNNNNNNGG 7 cut(s) 56, 113, 167, 615, 675, 731, 856
BseMII CTCAG 4 cut(s) 444, 519, 626, 1211
BseNI ACTGG 4 cut(s) 491, 648, 843, 885
BseRI GAGGAG 2 cut(s) 29, 212
BseSI GKGCMC 1 cut(s) 51
BseXI GCAGC 2 cut(s) 356, 495
BsgI GTGCAG 1 cut(s) 647
BshFI GGCC 4 cut(s) 49, 73, 277, 948
BsiHKAI GWGCWC 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 1130
BsiSI CCGG 3 cut(s) 70, 547, 578
BslFI GGGAC 3 cut(s) 129, 233, 831
BslI CCNNNNNNNGG 7 cut(s) 56, 113, 167, 615, 675, 731, 856
BsmAI GTCTC 3 cut(s) 524, 961, 1098
BsmFI GGGAC 3 cut(s) 129, 233, 831
BsnI GGCC 4 cut(s) 49, 73, 277, 948
Bso31I GGTCTC 1 cut(s) 1098
BsoBI CYCGRG 1 cut(s) 1130
Bsp120I GGGCCC 1 cut(s) 47
Bsp1286I GDGCHC 2 cut(s) 11, 51
Bsp143I GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
Bsp19I CCATGG 1 cut(s) 762
BspACI CCGC 5 cut(s) 113, 225, 380, 616, 669
BspANI GGCC 4 cut(s) 49, 73, 277, 948
BspCNI CTCAG 4 cut(s) 445, 520, 625, 1212
BspLI GGNNCC 3 cut(s) 49, 249, 949
BspMAI CTGCAG 1 cut(s) 374
BspPI GGATC 3 cut(s) 424, 482, 1142
BspTI CTTAAG 2 cut(s) 242, 634
BspTNI GGTCTC 1 cut(s) 1098
BsrI ACTGG 4 cut(s) 491, 648, 843, 885
BssECI CCNNGG 3 cut(s) 166, 762, 1144
BssMI GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
BssNI GRCGYC 1 cut(s) 640
BssT1I CCWWGG 2 cut(s) 762, 1144
Bst2UI CCWGG 4 cut(s) 167, 434, 792, 1103
Bst4CI ACNGT 3 cut(s) 78, 359, 1162
BstACI GRCGYC 1 cut(s) 640
BstAFI CTTAAG 2 cut(s) 242, 634
BstC8I GCNNGC 1 cut(s) 1056
BstDEI CTNAG 5 cut(s) 453, 528, 612, 1125, 1220
BstDSI CCRYGG 1 cut(s) 762
BstENI CCTNNNNNAGG 1 cut(s) 729
BstF5I GGATG 7 cut(s) 133, 426, 683, 874, 906, 910, 1073
BstKTI GATC 7 cut(s) 36, 316, 321, 432, 490, 709, 1137
BstMAI GTCTC 3 cut(s) 524, 961, 1098
BstMBI GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
BstMWI GCNNNNNNNGC 1 cut(s) 458
BstNI CCWGG 4 cut(s) 167, 434, 792, 1103
BstNSI RCATGY 1 cut(s) 1058
BstPAI GACNNNNGTC 1 cut(s) 536
BstSCI CCNGG 4 cut(s) 165, 432, 790, 1101
BstSFI CTRYAG 1 cut(s) 370
BstSLI GKGCMC 1 cut(s) 51
BstV1I GCAGC 2 cut(s) 356, 495
BstV2I GAAGAC 2 cut(s) 276, 333
BstX2I RGATCY 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 1109
BstYI RGATCY 1 cut(s) 313
BsuI GTATCC 2 cut(s) 232, 1109
BsuRI GGCC 4 cut(s) 49, 73, 277, 948
BtgI CCRYGG 1 cut(s) 762
BtsCI GGATG 7 cut(s) 133, 426, 683, 874, 906, 910, 1073
BtsIMutI CAGTG 2 cut(s) 74, 547
Cac8I GCNNGC 1 cut(s) 1056
Cfr13I GGNCC 4 cut(s) 47, 48, 247, 947
Csp6I GTAC 2 cut(s) 873, 926
CspCI CAANNNNNGTGG 4 cut(s) 63, 98, 676, 711
CviAII CATG 5 cut(s) 37, 281, 322, 763, 1055
CviQI GTAC 2 cut(s) 873, 926
DdeI CTNAG 5 cut(s) 453, 528, 612, 1125, 1220
DpnI GATC 7 cut(s) 35, 315, 320, 431, 489, 708, 1136
DpnII GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
DriI GACNNNNNGTC 1 cut(s) 334
EaeI YGGCCR 1 cut(s) 71
Eam1105I GACNNNNNGTC 1 cut(s) 334
Ecl136II GAGCTC 1 cut(s) 9
Eco130I CCWWGG 2 cut(s) 762, 1144
Eco24I GRGCYC 2 cut(s) 11, 51
Eco31I GGTCTC 1 cut(s) 1098
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 247
Eco53kI GAGCTC 1 cut(s) 9
Eco57I CTGAAG 1 cut(s) 292
Eco88I CYCGRG 1 cut(s) 1130
EcoICRI GAGCTC 1 cut(s) 9
EcoNI CCTNNNNNAGG 1 cut(s) 729
EcoO109I RGGNCCY 1 cut(s) 247
EcoRII CCWGG 4 cut(s) 165, 432, 790, 1101
EcoRV GATATC 1 cut(s) 715
EcoT14I CCWWGG 2 cut(s) 762, 1144
EcoT22I ATGCAT 1 cut(s) 1056
EcoT38I GRGCYC 2 cut(s) 11, 51
ErhI CCWWGG 2 cut(s) 762, 1144
FaeI CATG 5 cut(s) 40, 284, 325, 766, 1058
FaqI GGGAC 3 cut(s) 129, 233, 831
FatI CATG 5 cut(s) 36, 280, 321, 762, 1054
FauI CCCGC 1 cut(s) 106
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 2 cut(s) 33, 318
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 2 cut(s) 370, 509
FokI GGATG 7 cut(s) 120, 433, 670, 881, 893, 897, 1080
FriOI GRGCYC 2 cut(s) 11, 51
Fsp4HI GCNGC 2 cut(s) 370, 509
FspBI CTAG 2 cut(s) 63, 1107
GluI GCNGC 2 cut(s) 370, 509
HaeIII GGCC 4 cut(s) 49, 73, 277, 948
HapII CCGG 3 cut(s) 70, 547, 578
Hin1I GRCGYC 1 cut(s) 640
Hin1II CATG 5 cut(s) 40, 284, 325, 766, 1058
HinfI GANTC 4 cut(s) 66, 157, 455, 837
HpaII CCGG 3 cut(s) 70, 547, 578
HphI GGTGA 5 cut(s) 487, 832, 871, 908, 1160
Hpy166II GTNNAC 4 cut(s) 296, 565, 904, 928
Hpy188I TCNGA 6 cut(s) 13, 93, 311, 318, 836, 1221
Hpy188III TCNNGA 4 cut(s) 386, 710, 1132, 1229
Hpy8I GTNNAC 4 cut(s) 296, 565, 904, 928
Hpy99I CGWCG 1 cut(s) 340
HpyAV CCTTC 1 cut(s) 613
HpyCH4III ACNGT 3 cut(s) 78, 359, 1162
HpyCH4IV ACGT 1 cut(s) 640
HpyCH4V TGCA 9 cut(s) 372, 511, 664, 683, 731, 968, 1043, 1054, 1058
HpyF10VI GCNNNNNNNGC 1 cut(s) 458
HpyF3I CTNAG 5 cut(s) 453, 528, 612, 1125, 1220
HpySE526I ACGT 1 cut(s) 640
Hsp92I GRCGYC 1 cut(s) 640
Hsp92II CATG 5 cut(s) 40, 284, 325, 766, 1058
Ksp22I TGATCA 2 cut(s) 33, 318
Kzo9I GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
LmnI GCTCC 4 cut(s) 6, 199, 626, 983
Lsp1109I GCAGC 2 cut(s) 356, 495
LweI GCATC 1 cut(s) 692
MaeI CTAG 2 cut(s) 63, 1107
MaeII ACGT 1 cut(s) 640
MaeIII GTNAC 4 cut(s) 493, 542, 838, 1156
MalI GATC 7 cut(s) 35, 315, 320, 431, 489, 708, 1136
MboI GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
MboII GAAGA 5 cut(s) 281, 338, 1068, 1163, 1178
MflI RGATCY 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 11, 51
MluCI AATT 9 cut(s) 138, 553, 830, 942, 1029, 1059, 1114, 1120, 1239
MlyI GAGTC 2 cut(s) 464, 846
Mph1103I ATGCAT 1 cut(s) 1056
MroXI GAANNNNTTC 1 cut(s) 1239
MseI TTAA 5 cut(s) 243, 588, 635, 783, 1032
MslI CAYNNNNRTG 1 cut(s) 933
MspA1I CMGCKG 2 cut(s) 290, 616
MspCI CTTAAG 2 cut(s) 242, 634
MspI CCGG 3 cut(s) 70, 547, 578
MspR9I CCNGG 4 cut(s) 167, 434, 792, 1103
MvaI CCWGG 4 cut(s) 167, 434, 792, 1103
MwoI GCNNNNNNNGC 1 cut(s) 458
NcoI CCATGG 1 cut(s) 762
NdeI CATATG 1 cut(s) 754
NdeII GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
NlaIII CATG 5 cut(s) 40, 284, 325, 766, 1058
NlaIV GGNNCC 3 cut(s) 49, 249, 949
NmuCI GTSAC 4 cut(s) 493, 542, 838, 1156
NsiI ATGCAT 1 cut(s) 1056
NspI RCATGY 1 cut(s) 1058
OliI CACNNNNGTG 1 cut(s) 933
PaeI GCATGC 1 cut(s) 1058
PdmI GAANNNNTTC 1 cut(s) 1239
PfeI GAWTC 2 cut(s) 66, 157
PflFI GACNNNGTC 1 cut(s) 1022
PflMI CCANNNNNTGG 1 cut(s) 856
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 2 cut(s) 371, 510
PleI GAGTC 2 cut(s) 463, 845
PpsI GAGTC 2 cut(s) 463, 845
PpuMI RGGWCCY 1 cut(s) 247
PshAI GACNNNNGTC 1 cut(s) 536
PsiI TTATAA 1 cut(s) 303
Psp124BI GAGCTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 4 cut(s) 165, 432, 790, 1101
PspGI CCWGG 4 cut(s) 165, 432, 790, 1101
PspN4I GGNNCC 3 cut(s) 49, 249, 949
PspOMI GGGCCC 1 cut(s) 47
PspPI GGNCC 4 cut(s) 47, 48, 247, 947
PspPPI RGGWCCY 1 cut(s) 247
PstI CTGCAG 1 cut(s) 374
PsuI RGATCY 1 cut(s) 313
PsyI GACNNNGTC 1 cut(s) 1022
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 2 cut(s) 874, 927
RsaNI GTAC 2 cut(s) 873, 926
RseI CAYNNNNRTG 1 cut(s) 933
SacI GAGCTC 1 cut(s) 11
SaqAI TTAA 5 cut(s) 243, 588, 635, 783, 1032
SatI GCNGC 2 cut(s) 370, 509
Sau3AI GATC 7 cut(s) 33, 313, 318, 429, 487, 706, 1134
Sau96I GGNCC 4 cut(s) 47, 48, 247, 947
SchI GAGTC 2 cut(s) 464, 846
ScrFI CCNGG 4 cut(s) 167, 434, 792, 1103
SduI GDGCHC 2 cut(s) 11, 51
SfaNI GCATC 1 cut(s) 692
SfcI CTRYAG 1 cut(s) 370
SinI GGWCC 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 933
SmlI CTYRAG 3 cut(s) 242, 395, 634
SmoI CTYRAG 3 cut(s) 242, 395, 634
SphI GCATGC 1 cut(s) 1058
Sse9I AATT 9 cut(s) 138, 553, 830, 942, 1029, 1059, 1114, 1120, 1239
SsiI CCGC 5 cut(s) 113, 225, 380, 616, 669
SspI AATATT 1 cut(s) 88
SspMI CTAG 2 cut(s) 63, 1107
SstI GAGCTC 1 cut(s) 11
StyD4I CCNGG 4 cut(s) 165, 432, 790, 1101
StyI CCWWGG 2 cut(s) 762, 1144
TaaI ACNGT 3 cut(s) 78, 359, 1162
TaiI ACGT 1 cut(s) 643
TaqI TCGA 1 cut(s) 1026
TasI AATT 9 cut(s) 138, 553, 830, 942, 1029, 1059, 1114, 1120, 1239
TatI WGTACW 1 cut(s) 925
TfiI GAWTC 2 cut(s) 66, 157
Tru1I TTAA 5 cut(s) 243, 588, 635, 783, 1032
Tru9I TTAA 5 cut(s) 243, 588, 635, 783, 1032
TscAI CASTG 2 cut(s) 81, 547
TseFI GTSAC 4 cut(s) 493, 542, 838, 1156
TseI GCWGC 2 cut(s) 369, 508
Tsp45I GTSAC 4 cut(s) 493, 542, 838, 1156
TspDTI ATGAA 4 cut(s) 119, 516, 771, 1185
TspGWI ACGGA 1 cut(s) 639
TspRI CASTG 2 cut(s) 81, 547
Tth111I GACNNNGTC 1 cut(s) 1022
Van91I CCANNNNNTGG 1 cut(s) 856
Vha464I CTTAAG 2 cut(s) 242, 634
VpaK11BI GGWCC 1 cut(s) 247
XagI CCTNNNNNAGG 1 cut(s) 729
XceI RCATGY 1 cut(s) 1058
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 1239
XspI CTAG 2 cut(s) 63, 1107
ZraI GACGTC 1 cut(s) 641
Zsp2I ATGCAT 1 cut(s) 1056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.