MD07G1165100.v1.1

Protein of unknown function (DUF1295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0006054
Physical Location & Seq
Reverse (-)
23987897 .. 23989434
1538 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1165100.v1.1.491

Sequence Viewer

Length: 972 bp
ATGGCTGGAGGTAACTTGAAGAATGCAGTGATAGCATTTCTCGCTCCCCTTCCCTCCATTCTCTTCTACCTCATATTCCTCAGCAACCACCATAGCCAAACTAATGAGAGCTTTCTCTCTCCTCTCTGGGCATGGTGCTATCACCACCCTCTTCTCCTAGCCAACGTCTTCTTCTTCCTCAACGTCAACGTCCTCTTCTGGGTCATCAGCCAAGCTCTTTCCAGCCACTGGATGATCGATTTGTACTGGACTGTGATCCCTGTAATGCTCGTTCACTACTATCGATGTCACCCTCTGGCGGATTACAACCGGTGGCGGTCGACGATTGCAATTTCGCTGACTTGGGTTTGGAGCCTCAGGCTTTCCCACAACTACTTTAGGCGGGAAAATTGGCAATGGGGTGCCAGGGAGGACTGGCGGTTCACCGATATGAGCTCACAGTACGGCAAGCACTGGTGGTGGGTTTCCTTCTTCGCCGTTTACCTCTCTCAACAGGTTTTTATAATTGGGATATGCCTTCCATTCTATGTAATCCACTCAGTTAATGTGCCACTGAACATATGGGATTCTGTTGCCATCATAGTCTGCATTACTGGCATTGTTGTAGCATACTTAGCTGACACGCAGCTCCATGACTTTGTGAGCCGAAACAACAAGCTGAAAGAGCTTGGAAAGCCCGTTGTGCCGAATCTTGACGAAGGGTTGTGGCACTATTCGCGGCATCCCAACTACTTCGGAGAGCAATTGTGGTGGTGGGGATTAGTCATATTTGCATGGAACTTGGGACATGGATGGGCCTTTGTTGGGTCTCTGGTCAATACAATGTGTTTAGCTTATGTGACTATACTTGTCGAACGCCGAATGCTGAAACAAGATTACCGAGTAGAAGCATACAGGCGGTATCAGAAGACAACCTCAGTGTGGATACCTTGGTTCAAGTCATCTCCAGGAGGAAAAGATAAGAACGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0006629 GO:0008150 GO:0008152 GO:0008202 GO:0016229 GO:0016491 GO:0044238 GO:0055114 GO:0071704 GO:1901360
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1295

Protein Analysis

324

Amino Acids

38.23

Weight (kDa)

9.12

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1295 PF06966 66 - 305 4.6e-65 Protein of unknown function (DUF1295)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 503
AccB1I GGYRCC 1 cut(s) 401
AccB7I CCANNNNNTGG 1 cut(s) 228
AccI GTMKAC 1 cut(s) 320
AccII CGCG 1 cut(s) 718
AciI CCGC 6 cut(s) 299, 316, 382, 418, 718, 898
AclWI GGATC 1 cut(s) 250
AfaI GTAC 2 cut(s) 245, 443
AfiI CCNNNNNNNGG 5 cut(s) 199, 228, 298, 804, 921
AgeI ACCGGT 1 cut(s) 309
AgsI TTSAA 2 cut(s) 19, 937
AjnI CCWGG 2 cut(s) 404, 946
AjuI GAANNNNNNNTTGG 2 cut(s) 155, 187
AloI GAACNNNNNNTCC 2 cut(s) 404, 436
AluBI AGCT 8 cut(s) 111, 215, 435, 617, 628, 658, 667, 833
AluI AGCT 8 cut(s) 111, 215, 435, 617, 628, 658, 667, 833
Alw21I GWGCWC 1 cut(s) 437
Alw26I GTCTC 1 cut(s) 813
AlwI GGATC 1 cut(s) 250
AlwNI CAGNNNCTG 1 cut(s) 228
AoxI GGCC 1 cut(s) 795
ApeKI GCWGC 1 cut(s) 625
AsiGI ACCGGT 1 cut(s) 309
AspS9I GGNCC 1 cut(s) 795
AsuHPI GGTGA 3 cut(s) 134, 281, 415
AxyI CCTNAGG 1 cut(s) 356
BaeI ACNNNNGTAYC 2 cut(s) 917, 950
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 1 cut(s) 437
BbsI GAAGAC 2 cut(s) 160, 914
Bbv12I GWGCWC 1 cut(s) 437
BbvCI CCTCAGC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 637
BccI CCATC 2 cut(s) 584, 786
BceAI ACGGC 2 cut(s) 460, 461
BciT130I CCWGG 2 cut(s) 406, 948
BciVI GTATCC 1 cut(s) 918
BcoDI GTCTC 1 cut(s) 813
BfaI CTAG 1 cut(s) 158
BfuI GTATCC 1 cut(s) 918
BisI GCNGC 2 cut(s) 626, 719
BlsI GCNGC 2 cut(s) 627, 720
Bme1390I CCNGG 2 cut(s) 406, 948
BmgT120I GGNCC 1 cut(s) 795
BmiI GGNNCC 2 cut(s) 353, 403
BmrFI CCNGG 2 cut(s) 406, 948
BmsI GCATC 1 cut(s) 730
BpiI GAAGAC 2 cut(s) 160, 914
BpmI CTGGAG 2 cut(s) 27, 930
Bpu10I CCTNAGC 1 cut(s) 80
Bsa29I ATCGAT 2 cut(s) 237, 283
BsaI GGTCTC 1 cut(s) 813
BsaJI CCNNGG 2 cut(s) 405, 929
BsaWI WCCGGW 1 cut(s) 309
Bsc4I CCNNNNNNNGG 5 cut(s) 199, 228, 298, 804, 921
Bse118I RCCGGY 1 cut(s) 309
Bse1I ACTGG 5 cut(s) 233, 251, 419, 458, 598
Bse21I CCTNAGG 1 cut(s) 356
Bse3DI GCAATG 1 cut(s) 401
BseBI CCWGG 2 cut(s) 406, 948
BseCI ATCGAT 2 cut(s) 237, 283
BseDI CCNNGG 2 cut(s) 405, 929
BseGI GGATG 3 cut(s) 237, 721, 797
BseLI CCNNNNNNNGG 5 cut(s) 199, 228, 298, 804, 921
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 4 cut(s) 94, 370, 552, 930
BseNI ACTGG 5 cut(s) 233, 251, 419, 458, 598
BseRI GAGGAG 1 cut(s) 111
BseXI GCAGC 1 cut(s) 637
Bsh1236I CGCG 1 cut(s) 718
Bsh1285I CGRYCG 1 cut(s) 320
BshFI GGCC 1 cut(s) 797
BshNI GGYRCC 1 cut(s) 401
BshTI ACCGGT 1 cut(s) 309
BshVI ATCGAT 2 cut(s) 237, 283
BsiEI CGRYCG 1 cut(s) 320
BsiHKAI GWGCWC 1 cut(s) 437
BsiSI CCGG 1 cut(s) 310
BslFI GGGAC 1 cut(s) 798
BslI CCNNNNNNNGG 5 cut(s) 199, 228, 298, 804, 921
BsmAI GTCTC 1 cut(s) 813
BsmFI GGGAC 1 cut(s) 798
BsmI GAATGC 2 cut(s) 28, 867
BsnI GGCC 1 cut(s) 797
Bso31I GGTCTC 1 cut(s) 813
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 2 cut(s) 234, 255
BspACI CCGC 6 cut(s) 299, 316, 382, 418, 718, 898
BspANI GGCC 1 cut(s) 797
BspCNI CTCAG 4 cut(s) 93, 369, 551, 929
BspDI ATCGAT 2 cut(s) 237, 283
BspFNI CGCG 1 cut(s) 718
BspLI GGNNCC 2 cut(s) 353, 403
BspPI GGATC 1 cut(s) 250
BspT107I GGYRCC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 813
BsrDI GCAATG 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 309
BsrI ACTGG 5 cut(s) 233, 251, 419, 458, 598
BssAI RCCGGY 1 cut(s) 309
BssECI CCNNGG 2 cut(s) 405, 929
BssMI GATC 2 cut(s) 234, 255
BssT1I CCWWGG 1 cut(s) 929
Bst2UI CCWGG 2 cut(s) 406, 948
Bst4CI ACNGT 2 cut(s) 253, 441
Bst6I CTCTTC 3 cut(s) 68, 156, 200
BstC8I GCNNGC 1 cut(s) 449
BstDEI CTNAG 5 cut(s) 80, 356, 538, 613, 916
BstF5I GGATG 3 cut(s) 237, 721, 797
BstFNI CGCG 1 cut(s) 718
BstKTI GATC 2 cut(s) 237, 258
BstMAI GTCTC 1 cut(s) 813
BstMBI GATC 2 cut(s) 234, 255
BstMCI CGRYCG 1 cut(s) 320
BstMWI GCNNNNNNNGC 8 cut(s) 32, 41, 594, 614, 664, 673, 682, 715
BstNI CCWGG 2 cut(s) 406, 948
BstSCI CCNGG 2 cut(s) 404, 946
BstUI CGCG 1 cut(s) 718
BstV1I GCAGC 1 cut(s) 637
BstV2I GAAGAC 2 cut(s) 160, 914
Bsu15I ATCGAT 2 cut(s) 237, 283
Bsu36I CCTNAGG 1 cut(s) 356
BsuI GTATCC 1 cut(s) 918
BsuRI GGCC 1 cut(s) 797
BsuTUI ATCGAT 2 cut(s) 237, 283
BtsCI GGATG 3 cut(s) 237, 721, 797
BtsI GCAGTG 1 cut(s) 33
BtsIMutI CAGTG 5 cut(s) 33, 226, 451, 551, 924
Cac8I GCNNGC 1 cut(s) 449
CaiI CAGNNNCTG 1 cut(s) 228
Cfr10I RCCGGY 1 cut(s) 309
Cfr13I GGNCC 1 cut(s) 795
ClaI ATCGAT 2 cut(s) 237, 283
Csp6I GTAC 2 cut(s) 244, 442
CspAI ACCGGT 1 cut(s) 309
CspCI CAANNNNNGTGG 2 cut(s) 731, 766
CviAII CATG 4 cut(s) 132, 632, 774, 788
CviQI GTAC 2 cut(s) 244, 442
DdeI CTNAG 5 cut(s) 80, 356, 538, 613, 916
DpnI GATC 2 cut(s) 236, 257
DpnII GATC 2 cut(s) 234, 255
Eam1104I CTCTTC 3 cut(s) 68, 156, 200
EarI CTCTTC 3 cut(s) 68, 156, 200
EciI GGCGGA 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 929
Eco24I GRGCYC 1 cut(s) 437
Eco31I GGTCTC 1 cut(s) 813
Eco53kI GAGCTC 1 cut(s) 435
Eco81I CCTNAGG 1 cut(s) 356
EcoICRI GAGCTC 1 cut(s) 435
EcoRII CCWGG 2 cut(s) 404, 946
EcoT14I CCWWGG 1 cut(s) 929
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 929
FaeI CATG 4 cut(s) 135, 635, 777, 791
FaqI GGGAC 1 cut(s) 798
FatI CATG 4 cut(s) 131, 631, 773, 787
FauI CCCGC 1 cut(s) 375
FauNDI CATATG 1 cut(s) 560
FblI GTMKAC 1 cut(s) 320
Fnu4HI GCNGC 2 cut(s) 626, 719
FokI GGATG 3 cut(s) 244, 708, 804
FriOI GRGCYC 1 cut(s) 437
Fsp4HI GCNGC 2 cut(s) 626, 719
FspBI CTAG 1 cut(s) 158
GluI GCNGC 2 cut(s) 626, 719
GsuI CTGGAG 2 cut(s) 27, 930
HaeIII GGCC 1 cut(s) 797
HapII CCGG 1 cut(s) 310
Hin1II CATG 4 cut(s) 135, 635, 777, 791
HincII GTYRAC 2 cut(s) 187, 321
HindII GTYRAC 2 cut(s) 187, 321
HinfI GANTC 2 cut(s) 566, 688
HpaII CCGG 1 cut(s) 310
HphI GGTGA 3 cut(s) 134, 281, 415
Hpy166II GTNNAC 5 cut(s) 187, 274, 321, 423, 481
Hpy188I TCNGA 2 cut(s) 737, 906
Hpy188III TCNNGA 1 cut(s) 692
Hpy8I GTNNAC 5 cut(s) 187, 274, 321, 423, 481
Hpy99I CGWCG 1 cut(s) 325
HpyAV CCTTC 4 cut(s) 59, 478, 527, 692
HpyCH4III ACNGT 2 cut(s) 253, 441
HpyCH4IV ACGT 3 cut(s) 165, 183, 189
HpyCH4V TGCA 4 cut(s) 26, 329, 588, 773
HpyF10VI GCNNNNNNNGC 8 cut(s) 32, 41, 594, 614, 664, 673, 682, 715
HpyF3I CTNAG 5 cut(s) 80, 356, 538, 613, 916
HpySE526I ACGT 3 cut(s) 165, 183, 189
Hsp92II CATG 4 cut(s) 135, 635, 777, 791
Kzo9I GATC 2 cut(s) 234, 255
LmnI GCTCC 3 cut(s) 49, 351, 633
Lsp1109I GCAGC 1 cut(s) 637
LweI GCATC 1 cut(s) 730
MaeI CTAG 1 cut(s) 158
MaeII ACGT 3 cut(s) 165, 183, 189
MaeIII GTNAC 3 cut(s) 11, 287, 838
MalI GATC 2 cut(s) 236, 257
MboI GATC 2 cut(s) 234, 255
MboII GAAGA 9 cut(s) 31, 55, 143, 160, 163, 166, 187, 463, 919
MfeI CAATTG 1 cut(s) 743
MhlI GDGCHC 1 cut(s) 437
MluCI AATT 4 cut(s) 330, 388, 504, 743
MseI TTAA 2 cut(s) 543, 970
MslI CAYNNNNRTG 1 cut(s) 428
MspI CCGG 1 cut(s) 310
MspR9I CCNGG 2 cut(s) 406, 948
MunI CAATTG 1 cut(s) 743
Mva1269I GAATGC 2 cut(s) 28, 867
MvaI CCWGG 2 cut(s) 406, 948
MvnI CGCG 1 cut(s) 718
MwoI GCNNNNNNNGC 8 cut(s) 32, 41, 594, 614, 664, 673, 682, 715
NdeI CATATG 1 cut(s) 560
NdeII GATC 2 cut(s) 234, 255
NlaIII CATG 4 cut(s) 135, 635, 777, 791
NlaIV GGNNCC 2 cut(s) 353, 403
NmuCI GTSAC 2 cut(s) 287, 838
PctI GAATGC 2 cut(s) 28, 867
PfeI GAWTC 2 cut(s) 566, 688
PflMI CCANNNNNTGG 1 cut(s) 228
PfoI TCCNGGA 1 cut(s) 946
PinAI ACCGGT 1 cut(s) 309
PkrI GCNGC 2 cut(s) 627, 720
PsiI TTATAA 1 cut(s) 503
Psp124BI GAGCTC 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 404, 946
PspGI CCWGG 2 cut(s) 404, 946
PspN4I GGNNCC 2 cut(s) 353, 403
PspPI GGNCC 1 cut(s) 795
PsrI GAACNNNNNNTAC 2 cut(s) 255, 287
PstNI CAGNNNCTG 1 cut(s) 228
RsaI GTAC 2 cut(s) 245, 443
RsaNI GTAC 2 cut(s) 244, 442
RseI CAYNNNNRTG 1 cut(s) 428
SacI GAGCTC 1 cut(s) 437
SalI GTCGAC 1 cut(s) 319
SaqAI TTAA 2 cut(s) 543, 970
SatI GCNGC 2 cut(s) 626, 719
Sau3AI GATC 2 cut(s) 234, 255
Sau96I GGNCC 1 cut(s) 795
ScrFI CCNGG 2 cut(s) 406, 948
SduI GDGCHC 1 cut(s) 437
SfaNI GCATC 1 cut(s) 730
SmiMI CAYNNNNRTG 1 cut(s) 428
Sse9I AATT 4 cut(s) 330, 388, 504, 743
SsiI CCGC 6 cut(s) 299, 316, 382, 418, 718, 898
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 437
StyD4I CCNGG 2 cut(s) 404, 946
StyI CCWWGG 1 cut(s) 929
TaaI ACNGT 2 cut(s) 253, 441
TaiI ACGT 3 cut(s) 168, 186, 192
TaqI TCGA 4 cut(s) 237, 283, 320, 852
TasI AATT 4 cut(s) 330, 388, 504, 743
TatI WGTACW 1 cut(s) 243
TauI GCSGC 1 cut(s) 721
TfiI GAWTC 2 cut(s) 566, 688
Tru1I TTAA 2 cut(s) 543, 970
Tru9I TTAA 2 cut(s) 543, 970
TscAI CASTG 5 cut(s) 33, 233, 458, 558, 924
TseFI GTSAC 2 cut(s) 287, 838
TseI GCWGC 1 cut(s) 625
Tsp45I GTSAC 2 cut(s) 287, 838
TspRI CASTG 5 cut(s) 33, 233, 458, 558, 924
Van91I CCANNNNNTGG 1 cut(s) 228
XcmI CCANNNNNNNNNTGG 1 cut(s) 558
XmiI GTMKAC 1 cut(s) 320
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.