MD07G1225300.v1.1

Sorting and assembly machinery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0009225
Physical Location & Seq
Reverse (-)
30180249 .. 30184238
3990 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1225300.v1.1.491

Sequence Viewer

Length: 1620 bp
ATGGCAAAATCCGACTCCGAAACTCAAAACCCTAAGGACGACCGCGACGAGGAACCTCCCTCCAATGTCGACGGCACAGTCGACGGCGACCCCGAACCCGACGAAATGGATGACGAAATCGACGAAGAGGAAGACGAAGACGACGACGAGGAGCCACCTCTGCCACGAAAGCCTCTCTCTCAGGAGGCGCGGTTGCGCGCGGAGAAGTCCAAGCTAGAGAACCTGGTCCAGCGGATGTCGACCGAGCGAGTCGATCTGAGGGTCCACGACGTTCTGATCAAGGGCAACACGAAGACGAAGGAGCATTTGATCGAGGCCGAGCTGGCGGGGATTAAGAAGGCCACCACCATGCAAGAGCTGCTCGAGGCCGCCGCAATTGCAAATGCGAAGCTTCAGCAGCTTGAAATATTCGATGCGGTTCGGATCACCCTCGATTCTGGCCCGCCGGAGCTGCCTGGAACTACCAATGTTATTGTGGAGGTCGTCGAAACTAAGAGCCCGATTTCTGGCGAAATCGGTGCTTACACGAAGCCGGCGGCTAGGTCATGGACTGCTGAAGGAACACTGAAGTTCAAAAATCTGCTCGGATATGGGGATTTGTGGGATAGTTCTTTGGCATATGGGCCCAACCAAACGTCGGAGTTAAGTGCTGGTCTGTTTTTGCCTAGGTTCAAAGGGTTTGTTACTCCAATGACGGCACGAGCATTCCTGCTTTCCCAAGATTGGCTGGAATTCTCTTCTTATAAAGAGCGCATGATGGGGCTCTCTCTTGGACTGATTTCATCAAAACACCATGATTTGGCATACAATCTGGGTTGGCGAACCCTAGCAGACCCATCACAATTGGCCTCGAGGTCAATAAGAAGGCAGCTCGGCCATGAATTACTTTCTTCAATCAAATACACATTTAAGATTGATAGGAGAAATTCACCTTTGAGGCCAACTCGAGGTTACTCATTTGTTTCGACGAGTCATATTGGTGGCATTGCACCTGATCATCGAAGCACGAGGTTTTTGCGTCAGGAGTTCGATCTTCGTTATGCGGTTCCATTAGGATTTTATCATGCTGCACTTAATTTTGGGATCTCCGCAGGAGTTATTTTTCCATGGGGGAGTGGATTCTTGAACAAGCCCTCATCCCTTCCAGAGCGGTTCTTCCTGGGTGGTGATTTCTCTCCAGTTTGCACTGTTGGGGGCCCTACAACAGTGTGGGGATTCAAGACAAGGGGACTAGGCCCAACTGAACCAAGAAGGCATGTCGGAGATAATTCTAATGAGGAGAATTCAGAATCCCCTGGAAGAGATTCTGTTGGAGGAGATCTTGCTGTTTCTGCATTTGCAGACATTTCATTTGACCTTCCACTTCGCTGGTTAAAACAACATGGAGTGCATGGTCATATTTTCGCAGGTGCAGGGAATCTGGCGAAATTGACGGAGAACGAATTCCGAAACTTTTCTGCCCCGAAGTTCCTTCAGTCATTTCGAAGTTCTGTGGGAGCAGGGATTGTTGTCCCTACCAAGTTCTTCCGCCTAGAGTGCAACTACTACTATATACTGAAGCAATTTGAGCATGACCGTGGGAAGACTGGCTTTCGCTTCAGCATCTCTGCTCCGTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0008150 GO:0009987 GO:0016043 GO:0042407 GO:0061024 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bac_surface_Ag POTRA

Protein Analysis

540

Amino Acids

59.88

Weight (kDa)

5.66

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 193 - 536 4.9e-31 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 744
AarI CACCTGC 1 cut(s) 1397
AasI GACNNNNNNGTC 1 cut(s) 77
AbsI CCTCGAGG 1 cut(s) 850
Acc36I ACCTGC 1 cut(s) 1397
AccB7I CCANNNNNTGG 1 cut(s) 799
AccBSI CCGCTC 1 cut(s) 1150
AccI GTMKAC 3 cut(s) 69, 81, 239
AccII CGCG 4 cut(s) 45, 190, 198, 200
AclWI GGATC 2 cut(s) 431, 1091
AcoI YGGCCR 1 cut(s) 874
AcsI RAATTY 4 cut(s) 731, 925, 1282, 1442
AcuI CTGAAG 6 cut(s) 377, 576, 587, 1457, 1577, 1582
AfiI CCNNNNNNNGG 6 cut(s) 49, 506, 637, 723, 799, 947
AgsI TTSAA 6 cut(s) 404, 574, 673, 894, 1126, 1219
AjnI CCWGG 4 cut(s) 222, 454, 1158, 1294
AluBI AGCT 7 cut(s) 214, 322, 358, 391, 400, 451, 871
AluI AGCT 7 cut(s) 214, 322, 358, 391, 400, 451, 871
AlwI GGATC 2 cut(s) 431, 1091
Ama87I CYCGRG 3 cut(s) 362, 850, 945
ApaI GGGCCC 2 cut(s) 627, 1199
ApeKI GCWGC 5 cut(s) 358, 397, 451, 868, 1067
ApoI RAATTY 4 cut(s) 731, 925, 1282, 1442
ArsI GACNNNNNNTTYG 2 cut(s) 1334, 1366
Asp700I GAANNNNTTC 3 cut(s) 1303, 1442, 1453
AspA2I CCTAGG 1 cut(s) 665
AspLEI GCGC 4 cut(s) 190, 198, 200, 753
AspS9I GGNCC 8 cut(s) 226, 262, 440, 623, 624, 1195, 1196, 1235
AsuHPI GGTGA 3 cut(s) 418, 921, 1178
AsuII TTCGAA 1 cut(s) 1483
AvaI CYCGRG 3 cut(s) 362, 850, 945
AvaII GGWCC 2 cut(s) 226, 262
AvrII CCTAGG 1 cut(s) 665
AxyI CCTNAGG 1 cut(s) 33
BaeGI GKGCMC 2 cut(s) 627, 1199
BanII GRGCYC 4 cut(s) 500, 627, 765, 1199
BauI CACGAG 2 cut(s) 699, 1006
BbsI GAAGAC 4 cut(s) 138, 144, 299, 1589
BbvI GCAGC 5 cut(s) 345, 409, 438, 880, 1054
BccI CCATC 2 cut(s) 751, 844
BceAI ACGGC 3 cut(s) 88, 100, 711
BcgI CGANNNNNNTGC 4 cut(s) 500, 534, 997, 1031
BciT130I CCWGG 4 cut(s) 224, 456, 1160, 1296
BclI TGATCA 2 cut(s) 276, 994
BfaI CTAG 6 cut(s) 215, 540, 666, 827, 1232, 1532
BfuAI ACCTGC 1 cut(s) 1397
BglI GCCNNNNNGGC 1 cut(s) 323
BglII AGATCT 1 cut(s) 1318
BisI GCNGC 8 cut(s) 359, 369, 372, 398, 452, 537, 869, 1068
BlnI CCTAGG 1 cut(s) 665
BlsI GCNGC 8 cut(s) 360, 370, 373, 399, 453, 538, 870, 1069
Bme1390I CCNGG 4 cut(s) 224, 456, 1160, 1296
Bme18I GGWCC 2 cut(s) 226, 262
BmeT110I CYCGRG 3 cut(s) 362, 850, 945
BmgT120I GGNCC 8 cut(s) 226, 262, 440, 623, 624, 1195, 1196, 1235
BmiI GGNNCC 7 cut(s) 54, 153, 263, 625, 1047, 1196, 1197
BmrFI CCNGG 4 cut(s) 224, 456, 1160, 1296
BmsI GCATC 2 cut(s) 403, 1611
BpiI GAAGAC 4 cut(s) 138, 144, 299, 1589
BplI GAGNNNNNCTC 2 cut(s) 928, 960
BpmI CTGGAG 1 cut(s) 1161
Bpu14I TTCGAA 1 cut(s) 1483
BsaJI CCNNGG 5 cut(s) 665, 1106, 1159, 1294, 1576
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 506, 637, 723, 799, 947
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 2 cut(s) 1178, 1591
Bse21I CCTNAGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 984
BseBI CCWGG 4 cut(s) 224, 456, 1160, 1296
BseDI CCNNGG 5 cut(s) 665, 1106, 1159, 1294, 1576
BseGI GGATG 3 cut(s) 115, 240, 1136
BseLI CCNNNNNNNGG 6 cut(s) 49, 506, 637, 723, 799, 947
BseMI GCAATG 1 cut(s) 984
BseMII CTCAG 2 cut(s) 194, 248
BseNI ACTGG 2 cut(s) 1178, 1591
BsePI GCGCGC 1 cut(s) 196
BseRI GAGGAG 3 cut(s) 164, 1292, 1329
BseSI GKGCMC 2 cut(s) 627, 1199
BseXI GCAGC 5 cut(s) 345, 409, 438, 880, 1054
BsgI GTGCAG 2 cut(s) 1053, 1431
Bsh1236I CGCG 4 cut(s) 45, 190, 198, 200
Bsh1285I CGRYCG 2 cut(s) 43, 243
BsiEI CGRYCG 2 cut(s) 43, 243
BsiHKCI CYCGRG 3 cut(s) 362, 850, 945
BsiSI CCGG 2 cut(s) 446, 533
BslFI GGGAC 2 cut(s) 1242, 1496
BslI CCNNNNNNNGG 6 cut(s) 49, 506, 637, 723, 799, 947
BsmFI GGGAC 2 cut(s) 1242, 1496
BsmI GAATGC 1 cut(s) 704
BsoBI CYCGRG 3 cut(s) 362, 850, 945
Bsp119I TTCGAA 1 cut(s) 1483
Bsp120I GGGCCC 2 cut(s) 623, 1195
Bsp1286I GDGCHC 4 cut(s) 500, 627, 765, 1199
Bsp143I GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
Bsp19I CCATGG 1 cut(s) 1106
BspCNI CTCAG 2 cut(s) 193, 249
BspFNI CGCG 4 cut(s) 45, 190, 198, 200
BspLI GGNNCC 7 cut(s) 54, 153, 263, 625, 1047, 1196, 1197
BspMI ACCTGC 1 cut(s) 1397
BspPI GGATC 2 cut(s) 431, 1091
BspT104I TTCGAA 1 cut(s) 1483
BsrBI CCGCTC 1 cut(s) 1150
BsrDI GCAATG 1 cut(s) 984
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 2 cut(s) 1178, 1591
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 5 cut(s) 665, 1106, 1159, 1294, 1576
BssHII GCGCGC 1 cut(s) 196
BssMI GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
BssSI CACGAG 2 cut(s) 699, 1006
BssT1I CCWWGG 2 cut(s) 665, 1106
Bst2BI CACGAG 2 cut(s) 699, 1006
Bst2UI CCWGG 4 cut(s) 224, 456, 1160, 1296
Bst4CI ACNGT 4 cut(s) 79, 1189, 1207, 1577
Bst6I CTCTTC 3 cut(s) 120, 742, 1294
BstAPI GCANNNNNTGC 1 cut(s) 358
BstBI TTCGAA 1 cut(s) 1483
BstC8I GCNNGC 4 cut(s) 198, 324, 443, 534
BstDEI CTNAG 4 cut(s) 33, 180, 257, 492
BstDSI CCRYGG 2 cut(s) 1106, 1576
BstF5I GGATG 3 cut(s) 115, 240, 1136
BstFNI CGCG 4 cut(s) 45, 190, 198, 200
BstHHI GCGC 4 cut(s) 190, 198, 200, 753
BstKTI GATC 8 cut(s) 256, 279, 312, 426, 997, 1033, 1086, 1321
BstMBI GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
BstMCI CGRYCG 2 cut(s) 43, 243
BstNI CCWGG 4 cut(s) 224, 456, 1160, 1296
BstNSI RCATGY 1 cut(s) 1259
BstSCI CCNGG 4 cut(s) 222, 454, 1158, 1294
BstSLI GKGCMC 2 cut(s) 627, 1199
BstUI CGCG 4 cut(s) 45, 190, 198, 200
BstV1I GCAGC 5 cut(s) 345, 409, 438, 880, 1054
BstV2I GAAGAC 4 cut(s) 138, 144, 299, 1589
BstX2I RGATCY 2 cut(s) 1083, 1318
BstXI CCANNNNNNTGG 1 cut(s) 1368
BstYI RGATCY 2 cut(s) 1083, 1318
Bsu36I CCTNAGG 1 cut(s) 33
BtgI CCRYGG 2 cut(s) 1106, 1576
BtsCI GGATG 3 cut(s) 115, 240, 1136
BtsIMutI CAGTG 3 cut(s) 563, 1185, 1212
BveI ACCTGC 1 cut(s) 1397
Cac8I GCNNGC 4 cut(s) 198, 324, 443, 534
CfoI GCGC 4 cut(s) 190, 198, 200, 753
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 8 cut(s) 226, 262, 440, 623, 624, 1195, 1196, 1235
CseI GACGC 1 cut(s) 1007
CsiI ACCWGGT 1 cut(s) 222
DdeI CTNAG 4 cut(s) 33, 180, 257, 492
DpnI GATC 8 cut(s) 255, 278, 311, 425, 996, 1032, 1085, 1320
DpnII GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
DrdI GACNNNNNNGTC 1 cut(s) 77
DseDI GACNNNNNNGTC 1 cut(s) 77
EaeI YGGCCR 1 cut(s) 874
Eam1104I CTCTTC 3 cut(s) 120, 742, 1294
EarI CTCTTC 3 cut(s) 120, 742, 1294
EciI GGCGGA 1 cut(s) 1517
Eco130I CCWWGG 2 cut(s) 665, 1106
Eco24I GRGCYC 4 cut(s) 500, 627, 765, 1199
Eco47I GGWCC 2 cut(s) 226, 262
Eco57I CTGAAG 6 cut(s) 377, 576, 587, 1457, 1577, 1582
Eco81I CCTNAGG 1 cut(s) 33
Eco88I CYCGRG 3 cut(s) 362, 850, 945
EcoO109I RGGNCCY 2 cut(s) 1195, 1196
EcoRI GAATTC 3 cut(s) 731, 1282, 1442
EcoRII CCWGG 4 cut(s) 222, 454, 1158, 1294
EcoT14I CCWWGG 2 cut(s) 665, 1106
EcoT38I GRGCYC 4 cut(s) 500, 627, 765, 1199
ErhI CCWWGG 2 cut(s) 665, 1106
FalI AAGNNNNNCTT 2 cut(s) 1574, 1606
FaqI GGGAC 2 cut(s) 1242, 1496
FauI CCCGC 2 cut(s) 319, 450
FauNDI CATATG 1 cut(s) 619
FbaI TGATCA 2 cut(s) 276, 994
FblI GTMKAC 3 cut(s) 69, 81, 239
Fnu4HI GCNGC 8 cut(s) 359, 369, 372, 398, 452, 537, 869, 1068
FokI GGATG 3 cut(s) 122, 247, 1123
FriOI GRGCYC 4 cut(s) 500, 627, 765, 1199
Fsp4HI GCNGC 8 cut(s) 359, 369, 372, 398, 452, 537, 869, 1068
FspBI CTAG 6 cut(s) 215, 540, 666, 827, 1232, 1532
GlaI GCGC 4 cut(s) 189, 197, 199, 752
GluI GCNGC 8 cut(s) 359, 369, 372, 398, 452, 537, 869, 1068
GsuI CTGGAG 1 cut(s) 1161
HapII CCGG 2 cut(s) 446, 533
HgaI GACGC 1 cut(s) 1007
HhaI GCGC 4 cut(s) 190, 198, 200, 753
Hin6I GCGC 4 cut(s) 188, 196, 198, 751
HinP1I GCGC 4 cut(s) 188, 196, 198, 751
HincII GTYRAC 3 cut(s) 70, 82, 240
HindII GTYRAC 3 cut(s) 70, 82, 240
HindIII AAGCTT 1 cut(s) 389
HinfI GANTC 9 cut(s) 14, 249, 434, 970, 1119, 1215, 1289, 1304, 1417
HpaII CCGG 2 cut(s) 446, 533
HphI GGTGA 3 cut(s) 418, 921, 1178
Hpy166II GTNNAC 4 cut(s) 70, 82, 240, 265
Hpy188III TCNNGA 5 cut(s) 182, 1022, 1123, 1145, 1219
Hpy8I GTNNAC 4 cut(s) 70, 82, 240, 265
HpyAV CCTTC 8 cut(s) 292, 331, 551, 858, 1151, 1245, 1367, 1481
HpyCH4III ACNGT 4 cut(s) 79, 1189, 1207, 1577
HpyCH4IV ACGT 2 cut(s) 270, 635
HpyF3I CTNAG 4 cut(s) 33, 180, 257, 492
HpySE526I ACGT 2 cut(s) 270, 635
HspAI GCGC 4 cut(s) 188, 196, 198, 751
KroI GCCGGC 1 cut(s) 532
KroNI GCCGGC 1 cut(s) 534
Ksp22I TGATCA 2 cut(s) 276, 994
Kzo9I GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
LmnI GCTCC 5 cut(s) 151, 301, 448, 1496, 1615
Lsp1109I GCAGC 5 cut(s) 345, 409, 438, 880, 1054
LweI GCATC 2 cut(s) 403, 1611
MabI ACCWGGT 1 cut(s) 222
MaeI CTAG 6 cut(s) 215, 540, 666, 827, 1232, 1532
MaeII ACGT 2 cut(s) 270, 635
MaeIII GTNAC 2 cut(s) 682, 950
MalI GATC 8 cut(s) 255, 278, 311, 425, 996, 1032, 1085, 1320
MbiI CCGCTC 1 cut(s) 1150
MboI GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
MfeI CAATTG 2 cut(s) 375, 842
MflI RGATCY 2 cut(s) 1083, 1318
MhlI GDGCHC 4 cut(s) 500, 627, 765, 1199
MlyI GAGTC 3 cut(s) 8, 258, 979
MmeI TCCRAC 4 cut(s) 36, 618, 1240, 1291
MroNI GCCGGC 1 cut(s) 532
MroXI GAANNNNTTC 3 cut(s) 1303, 1442, 1453
MseI TTAA 5 cut(s) 333, 644, 909, 1074, 1373
MslI CAYNNNNRTG 3 cut(s) 347, 978, 1575
MspA1I CMGCKG 1 cut(s) 232
MspI CCGG 2 cut(s) 446, 533
MspR9I CCNGG 4 cut(s) 224, 456, 1160, 1296
MunI CAATTG 2 cut(s) 375, 842
Mva1269I GAATGC 1 cut(s) 704
MvaI CCWGG 4 cut(s) 224, 456, 1160, 1296
MvnI CGCG 4 cut(s) 45, 190, 198, 200
NaeI GCCGGC 1 cut(s) 534
NcoI CCATGG 1 cut(s) 1106
NdeI CATATG 1 cut(s) 619
NdeII GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
NgoMIV GCCGGC 1 cut(s) 532
NlaIV GGNNCC 7 cut(s) 54, 153, 263, 625, 1047, 1196, 1197
NmeAIII GCCGAG 2 cut(s) 343, 852
NspI RCATGY 1 cut(s) 1259
NspV TTCGAA 1 cut(s) 1483
PaeR7I CTCGAG 3 cut(s) 362, 850, 945
PaqCI CACCTGC 1 cut(s) 1397
PauI GCGCGC 1 cut(s) 196
PcsI WCGNNNNNNNCGW 5 cut(s) 45, 78, 90, 120, 141
PctI GAATGC 1 cut(s) 704
PdiI GCCGGC 1 cut(s) 534
PdmI GAANNNNTTC 3 cut(s) 1303, 1442, 1453
PfeI GAWTC 6 cut(s) 434, 1119, 1215, 1289, 1304, 1417
PflMI CCANNNNNTGG 1 cut(s) 799
PkrI GCNGC 8 cut(s) 360, 370, 373, 399, 453, 538, 870, 1069
PleI GAGTC 3 cut(s) 8, 257, 978
PpsI GAGTC 3 cut(s) 8, 257, 978
PsiI TTATAA 1 cut(s) 744
Psp6I CCWGG 4 cut(s) 222, 454, 1158, 1294
PspGI CCWGG 4 cut(s) 222, 454, 1158, 1294
PspN4I GGNNCC 7 cut(s) 54, 153, 263, 625, 1047, 1196, 1197
PspOMI GGGCCC 2 cut(s) 623, 1195
PspPI GGNCC 8 cut(s) 226, 262, 440, 623, 624, 1195, 1196, 1235
PspXI VCTCGAGB 3 cut(s) 362, 850, 945
PsuI RGATCY 2 cut(s) 1083, 1318
PteI GCGCGC 1 cut(s) 196
RseI CAYNNNNRTG 3 cut(s) 347, 978, 1575
SalI GTCGAC 3 cut(s) 68, 80, 238
SaqAI TTAA 5 cut(s) 333, 644, 909, 1074, 1373
SatI GCNGC 8 cut(s) 359, 369, 372, 398, 452, 537, 869, 1068
Sau3AI GATC 8 cut(s) 253, 276, 309, 423, 994, 1030, 1083, 1318
Sau96I GGNCC 8 cut(s) 226, 262, 440, 623, 624, 1195, 1196, 1235
SchI GAGTC 3 cut(s) 8, 258, 979
ScrFI CCNGG 4 cut(s) 224, 456, 1160, 1296
SduI GDGCHC 4 cut(s) 500, 627, 765, 1199
SexAI ACCWGGT 1 cut(s) 222
SfaNI GCATC 2 cut(s) 403, 1611
Sfr274I CTCGAG 3 cut(s) 362, 850, 945
SfuI TTCGAA 1 cut(s) 1483
SinI GGWCC 2 cut(s) 226, 262
SlaI CTCGAG 3 cut(s) 362, 850, 945
SmiMI CAYNNNNRTG 3 cut(s) 347, 978, 1575
SmlI CTYRAG 3 cut(s) 362, 850, 945
SmoI CTYRAG 3 cut(s) 362, 850, 945
SspI AATATT 1 cut(s) 408
SspMI CTAG 6 cut(s) 215, 540, 666, 827, 1232, 1532
StyD4I CCNGG 4 cut(s) 222, 454, 1158, 1294
StyI CCWWGG 2 cut(s) 665, 1106
TaaI ACNGT 4 cut(s) 79, 1189, 1207, 1577
TaiI ACGT 2 cut(s) 273, 638
TaqII GACCGA 1 cut(s) 257
TauI GCSGC 3 cut(s) 371, 374, 539
TfiI GAWTC 6 cut(s) 434, 1119, 1215, 1289, 1304, 1417
Tru1I TTAA 5 cut(s) 333, 644, 909, 1074, 1373
Tru9I TTAA 5 cut(s) 333, 644, 909, 1074, 1373
TscAI CASTG 3 cut(s) 570, 1192, 1212
TseI GCWGC 5 cut(s) 358, 397, 451, 868, 1067
TspDTI ATGAA 3 cut(s) 771, 894, 1338
TspGWI ACGGA 2 cut(s) 1448, 1602
TspRI CASTG 3 cut(s) 570, 1192, 1212
Van91I CCANNNNNTGG 1 cut(s) 799
VpaK11BI GGWCC 2 cut(s) 226, 262
XapI RAATTY 4 cut(s) 731, 925, 1282, 1442
XceI RCATGY 1 cut(s) 1259
XcmI CCANNNNNNNNNTGG 1 cut(s) 472
XhoI CTCGAG 3 cut(s) 362, 850, 945
XmaJI CCTAGG 1 cut(s) 665
XmiI GTMKAC 3 cut(s) 69, 81, 239
XmnI GAANNNNTTC 3 cut(s) 1303, 1442, 1453
XspI CTAG 6 cut(s) 215, 540, 666, 827, 1232, 1532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.