Rw1G032460

Sorting and assembly machinery

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0009225
Physical Location & Seq
Reverse (-)
60716174 .. 60719988
3815 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G032460.1

Sequence Viewer

Length: 1635 bp
ATGGCGGACTCTGAAAACCTAAACCCTAAAGTCGACCCCGAACAACAACCCCCTTCCAATCTCAACCAAAACGGACACCACGACGCCGACCCGGAGGAACCCGATGACGACGAATTCGAAGAAGAAGCAGACGAAGAAGACGACGACGAGGACGACGACGAACCGCCGCCTCCGCTGCAGCGAAAGCCGTCCTCGGAGGAGTCACTGCGTTCGTCGAAGGCCAAGCTGGAGGGCCTGGTCCGTCGGATGGAGACGGAGAAAGTCGATCTGAGGGTGCACGATGTTGTGATCAGAGGGAACACCAAGACCAAGGAGTATCTGATCGAGGCCGAGCTCGAGGGGATTAGGAAGGCCACGACTATGCAGGAGCTTCTCGAGGCCTCGGCTAAGGCCAATGCCAAGCTTAAAGAGCTCGATATCTTCGATTCCGTTCGGATCACGCTCGATTCCGGCCCGCCGGAGCTGCCGCGAACCGCCAACGTCCTTGTCGACGTCGTCGAGACTGAGAGTGTGATTACTGGTGAACTCGGTTGTTACACTAAGCCGGCGGCTAGATCTTGGACAGCTGAGGGTTCACTCAAGTTTAAAAATTTGTTTGGATATGGGGATATGTGGGATGGTTCTTTGGCATATGGTCCCAGCCAAACATCGGAAATAAGTGCTGGTGTGCTTTTACCCCGGTTCAAAGGATTTGTAACTCCTGTGACAGCACGTGTATTCCTTCTTTCCCAAGATTGGCTGGAGTTATCCTCTTATAAAGAGCGAATGGCTGGGCTCTCACTTAGCTTATTTTCAACCAGGCATCATGATGTTGAATACAACCTTGGTTGGCGTACACTTACAGATCCATCACAAATGGCCTCCAAGTCAATTAGAAGGCAGCTTGGCCATGGCTTACTTTCATCCATAAAGTACACATTTACACTTGATAGGAGAAATTCATATTTGAGGCCAACTCGAGGCTATTCTTTTGTTTCTTCTAGTCATGTTGGTGGCCTTTCCCCTGATAGTCGGAGTGTCCGGTTTTTGCGCCAGGAGCTTGATCTTCGTTATGCAGTCCCATTTGGATTTTATCATGCTGCACTCAACTTTGGGGTCTCTGGGGGAGTGGTCTTTCCTTGGGGGAATGGGTTTTTGAACAAGCCATCATCTCTTCCAGAGCGGTTCTTCTTAGGTGGTGATTTCTCTCCTGTTTGCACTCCTGGGGGCCCTGCAACAGTGTGGGGATTTAAAACAAGAGCACTAGGCCCTACTGAACCAAGAAGGCATGTCAGAGATAATTCTAATGATGAGAATTCAGAATCCTCTGGAAGGGATTTTGTTGGAGGAGATCTTGCTCTTTCTGCATTTGCAGACATTTCATTTGACCTTCCATTGCGCTGGTTAAGAGAACGCGGAGTTCATGGACATGTTTTTGCGGGAGCGGGGAATGTAGCAAAACTGTCGGAGAACGAGTTCCGAAAATTTTCCTTGCAGAGGTTCTTTGAGTCATTTCGAAGTTCAGTTGGAGCTGGGATTGTCATCCCCACAAAATTATTTCGCCTTGAGTGCAACTACTACTATATCCTGAAGCAATTTGAGCATGATGGCGGAAAGACTGGCTTTCGGTTTAGCATCTCAGCTCCATCATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006996 GO:0007005 GO:0007006 GO:0007007 GO:0008150 GO:0009987 GO:0016043 GO:0042407 GO:0061024 GO:0071840
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Bac_surface_Ag POTRA

Protein Analysis

544

Amino Acids

60.71

Weight (kDa)

5.37

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Omp85 PF01103 197 - 540 1.1e-28 Omp85 superfamily domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 756
AatII GACGTC 1 cut(s) 495
AccBSI CCGCTC 2 cut(s) 1162, 1424
AccI GTMKAC 2 cut(s) 33, 489
AccII CGCG 2 cut(s) 469, 1395
AclWI GGATC 2 cut(s) 443, 839
AcoI YGGCCR 1 cut(s) 886
AcsI RAATTY 5 cut(s) 113, 589, 937, 1294, 1463
AcuI CTGAAG 1 cut(s) 1589
AcvI CACGTG 1 cut(s) 713
AcyI GRCGYC 2 cut(s) 84, 492
AfaI GTAC 2 cut(s) 835, 914
AfiI CCNNNNNNNGG 6 cut(s) 247, 649, 735, 959, 1311, 1476
AflIII ACRYGT 2 cut(s) 712, 1408
AgsI TTSAA 4 cut(s) 685, 795, 815, 1138
AjnI CCWGG 4 cut(s) 234, 797, 1032, 1201
AjuI GAANNNNNNNTTGG 2 cut(s) 807, 839
AloI GAACNNNNNNTCC 2 cut(s) 1439, 1471
Alw21I GWGCWC 4 cut(s) 279, 336, 414, 1243
Alw26I GTCTC 3 cut(s) 245, 494, 1102
Alw44I GTGCAC 1 cut(s) 275
AlwI GGATC 2 cut(s) 443, 839
Ama87I CYCGRG 3 cut(s) 335, 374, 957
ApaI GGGCCC 1 cut(s) 1211
ApaLI GTGCAC 1 cut(s) 275
ApeKI GCWGC 5 cut(s) 175, 178, 463, 880, 1079
ApoI RAATTY 5 cut(s) 113, 589, 937, 1294, 1463
ArsI GACNNNNNNTTYG 4 cut(s) 98, 130, 1346, 1378
Asp700I GAANNNNTTC 2 cut(s) 1454, 1465
AspLEI GCGC 2 cut(s) 1032, 1380
AspS9I GGNCC 7 cut(s) 232, 238, 452, 635, 1207, 1208, 1247
AsuC2I CCSGG 2 cut(s) 92, 679
AsuHPI GGTGA 2 cut(s) 533, 1190
AsuII TTCGAA 2 cut(s) 117, 1495
AvaI CYCGRG 3 cut(s) 335, 374, 957
AvaII GGWCC 2 cut(s) 238, 635
BaeGI GKGCMC 2 cut(s) 279, 1211
BalI TGGCCA 1 cut(s) 888
BanII GRGCYC 4 cut(s) 336, 414, 777, 1211
BbrPI CACGTG 1 cut(s) 713
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 4 cut(s) 279, 336, 414, 1243
BbvCI CCTCAGC 1 cut(s) 567
BbvI GCAGC 5 cut(s) 162, 190, 450, 892, 1066
BccI CCATC 6 cut(s) 241, 611, 856, 1153, 1580, 1633
BceAI ACGGC 1 cut(s) 172
BcgI CGANNNNNNTGC 2 cut(s) 1425, 1459
BciT130I CCWGG 4 cut(s) 236, 799, 1034, 1203
BclI TGATCA 1 cut(s) 288
BcnI CCSGG 2 cut(s) 92, 679
BcoDI GTCTC 3 cut(s) 245, 494, 1102
BfaI CTAG 3 cut(s) 552, 981, 1244
BfmI CTRYAG 1 cut(s) 176
BglII AGATCT 2 cut(s) 554, 1330
BisI GCNGC 8 cut(s) 167, 176, 179, 464, 467, 549, 881, 1080
BlsI GCNGC 8 cut(s) 168, 177, 180, 465, 468, 550, 882, 1081
Bme1390I CCNGG 6 cut(s) 92, 236, 679, 799, 1034, 1203
Bme18I GGWCC 2 cut(s) 238, 635
BmeT110I CYCGRG 3 cut(s) 335, 374, 957
BmgT120I GGNCC 7 cut(s) 232, 238, 452, 635, 1207, 1208, 1247
BmiI GGNNCC 4 cut(s) 99, 637, 1208, 1209
BmrFI CCNGG 6 cut(s) 92, 236, 679, 799, 1034, 1203
BmsI GCATC 2 cut(s) 811, 1623
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 4 cut(s) 734, 766, 940, 972
BpmI CTGGAG 2 cut(s) 248, 761
Bpu10I CCTNAGC 2 cut(s) 387, 567
Bpu14I TTCGAA 2 cut(s) 117, 1495
BpuEI CTTGAG 2 cut(s) 563, 1565
BpuMI CCSGG 2 cut(s) 92, 679
BsaAI YACGTR 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 1520
BsaHI GRCGYC 2 cut(s) 84, 492
BsaI GGTCTC 1 cut(s) 1102
BsaJI CCNNGG 8 cut(s) 192, 309, 381, 677, 823, 889, 1118, 1202
BsaWI WCCGGW 1 cut(s) 1020
BsaXI ACNNNNNCTCC 2 cut(s) 1439, 1469
Bsc4I CCNNNNNNNGG 6 cut(s) 247, 649, 735, 959, 1311, 1476
Bse118I RCCGGY 1 cut(s) 544
Bse1I ACTGG 2 cut(s) 523, 1603
Bse3DI GCAATG 1 cut(s) 1373
Bse8I GATNNNNATC 1 cut(s) 1520
BseBI CCWGG 4 cut(s) 236, 799, 1034, 1203
BseDI CCNNGG 8 cut(s) 192, 309, 381, 677, 823, 889, 1118, 1202
BseGI GGATG 4 cut(s) 252, 622, 902, 1521
BseJI GATNNNNATC 1 cut(s) 1520
BseLI CCNNNNNNNGG 6 cut(s) 247, 649, 735, 959, 1311, 1476
BseMI GCAATG 1 cut(s) 1373
BseMII CTCAG 4 cut(s) 260, 495, 558, 1632
BseNI ACTGG 2 cut(s) 523, 1603
BseRI GAGGAG 2 cut(s) 212, 1341
BseSI GKGCMC 2 cut(s) 279, 1211
BseXI GCAGC 5 cut(s) 162, 190, 450, 892, 1066
BseYI CCCAGC 3 cut(s) 638, 770, 1511
BsgI GTGCAG 1 cut(s) 1065
Bsh1236I CGCG 2 cut(s) 469, 1395
BsiHKAI GWGCWC 4 cut(s) 279, 336, 414, 1243
BsiHKCI CYCGRG 3 cut(s) 335, 374, 957
BsiSI CCGG 6 cut(s) 92, 450, 458, 545, 679, 1021
BslFI GGGAC 2 cut(s) 621, 1043
BslI CCNNNNNNNGG 6 cut(s) 247, 649, 735, 959, 1311, 1476
BsmAI GTCTC 3 cut(s) 245, 494, 1102
BsmBI CGTCTC 1 cut(s) 245
BsmFI GGGAC 2 cut(s) 621, 1043
Bso31I GGTCTC 1 cut(s) 1102
BsoBI CYCGRG 3 cut(s) 335, 374, 957
Bsp119I TTCGAA 2 cut(s) 117, 1495
Bsp120I GGGCCC 1 cut(s) 1207
Bsp1286I GDGCHC 6 cut(s) 279, 336, 414, 777, 1211, 1243
Bsp143I GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
Bsp19I CCATGG 1 cut(s) 889
BspCNI CTCAG 4 cut(s) 261, 496, 559, 1631
BspFNI CGCG 2 cut(s) 469, 1395
BspHI TCATGA 1 cut(s) 805
BspLI GGNNCC 4 cut(s) 99, 637, 1208, 1209
BspMAI CTGCAG 1 cut(s) 180
BspPI GGATC 2 cut(s) 443, 839
BspT104I TTCGAA 2 cut(s) 117, 1495
BspTNI GGTCTC 1 cut(s) 1102
BsrBI CCGCTC 2 cut(s) 1162, 1424
BsrDI GCAATG 1 cut(s) 1373
BsrFI RCCGGY 1 cut(s) 544
BsrI ACTGG 2 cut(s) 523, 1603
BssAI RCCGGY 1 cut(s) 544
BssECI CCNNGG 8 cut(s) 192, 309, 381, 677, 823, 889, 1118, 1202
BssMI GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
BssNI GRCGYC 2 cut(s) 84, 492
BssT1I CCWWGG 4 cut(s) 309, 823, 889, 1118
Bst2UI CCWGG 4 cut(s) 236, 799, 1034, 1203
Bst4CI ACNGT 2 cut(s) 1219, 1443
Bst6I CTCTTC 1 cut(s) 1158
BstACI GRCGYC 2 cut(s) 84, 492
BstBAI YACGTR 1 cut(s) 713
BstBI TTCGAA 2 cut(s) 117, 1495
BstC8I GCNNGC 2 cut(s) 455, 546
BstDEI CTNAG 8 cut(s) 269, 387, 504, 540, 567, 782, 1171, 1618
BstDSI CCRYGG 1 cut(s) 889
BstENI CCTNNNNNAGG 2 cut(s) 1309, 1474
BstF5I GGATG 4 cut(s) 252, 622, 902, 1521
BstFNI CGCG 2 cut(s) 469, 1395
BstHHI GCGC 2 cut(s) 1032, 1380
BstKTI GATC 8 cut(s) 268, 291, 324, 438, 557, 847, 1045, 1333
BstMAI GTCTC 3 cut(s) 245, 494, 1102
BstMBI GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
BstMWI GCNNNNNNNGC 9 cut(s) 172, 175, 184, 409, 463, 1036, 1343, 1548, 1579
BstNI CCWGG 4 cut(s) 236, 799, 1034, 1203
BstNSI RCATGY 2 cut(s) 1271, 1412
BstSCI CCNGG 6 cut(s) 90, 234, 677, 797, 1032, 1201
BstSFI CTRYAG 1 cut(s) 176
BstSLI GKGCMC 2 cut(s) 279, 1211
BstUI CGCG 2 cut(s) 469, 1395
BstV1I GCAGC 5 cut(s) 162, 190, 450, 892, 1066
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 3 cut(s) 554, 844, 1330
BstXI CCANNNNNNTGG 1 cut(s) 1380
BstYI RGATCY 3 cut(s) 554, 844, 1330
BtgI CCRYGG 1 cut(s) 889
BtsCI GGATG 4 cut(s) 252, 622, 902, 1521
BtsI GCAGTG 1 cut(s) 203
BtsIMutI CAGTG 2 cut(s) 203, 1224
Cac8I GCNNGC 2 cut(s) 455, 546
CciI TCATGA 1 cut(s) 805
CfoI GCGC 2 cut(s) 1032, 1380
Cfr10I RCCGGY 1 cut(s) 544
Cfr13I GGNCC 7 cut(s) 232, 238, 452, 635, 1207, 1208, 1247
CseI GACGC 1 cut(s) 92
Csp6I GTAC 2 cut(s) 834, 913
CviAII CATG 8 cut(s) 806, 890, 986, 1076, 1268, 1403, 1409, 1583
CviQI GTAC 2 cut(s) 834, 913
DdeI CTNAG 8 cut(s) 269, 387, 504, 540, 567, 782, 1171, 1618
DpnI GATC 8 cut(s) 267, 290, 323, 437, 556, 846, 1044, 1332
DpnII GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
DraI TTTAAA 2 cut(s) 586, 1231
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 1 cut(s) 1158
EarI CTCTTC 1 cut(s) 1158
EciI GGCGGA 2 cut(s) 20, 1605
Ecl136II GAGCTC 2 cut(s) 334, 412
Eco130I CCWWGG 4 cut(s) 309, 823, 889, 1118
Eco147I AGGCCT 1 cut(s) 380
Eco24I GRGCYC 4 cut(s) 336, 414, 777, 1211
Eco31I GGTCTC 1 cut(s) 1102
Eco32I GATATC 1 cut(s) 418
Eco47I GGWCC 2 cut(s) 238, 635
Eco53kI GAGCTC 2 cut(s) 334, 412
Eco57I CTGAAG 1 cut(s) 1589
Eco72I CACGTG 1 cut(s) 713
Eco88I CYCGRG 3 cut(s) 335, 374, 957
EcoICRI GAGCTC 2 cut(s) 334, 412
EcoNI CCTNNNNNAGG 2 cut(s) 1309, 1474
EcoO109I RGGNCCY 4 cut(s) 232, 1207, 1208, 1247
EcoRI GAATTC 2 cut(s) 113, 1294
EcoRII CCWGG 4 cut(s) 234, 797, 1032, 1201
EcoRV GATATC 1 cut(s) 418
EcoT14I CCWWGG 4 cut(s) 309, 823, 889, 1118
EcoT38I GRGCYC 4 cut(s) 336, 414, 777, 1211
ErhI CCWWGG 4 cut(s) 309, 823, 889, 1118
Esp3I CGTCTC 1 cut(s) 245
FaeI CATG 8 cut(s) 809, 893, 989, 1079, 1271, 1406, 1412, 1586
FalI AAGNNNNNCTT 2 cut(s) 1586, 1618
FaqI GGGAC 2 cut(s) 621, 1043
FatI CATG 8 cut(s) 805, 889, 985, 1075, 1267, 1402, 1408, 1582
FauI CCCGC 3 cut(s) 462, 1411, 1417
FauNDI CATATG 1 cut(s) 631
FbaI TGATCA 1 cut(s) 288
FblI GTMKAC 2 cut(s) 33, 489
Fnu4HI GCNGC 8 cut(s) 167, 176, 179, 464, 467, 549, 881, 1080
FokI GGATG 4 cut(s) 259, 629, 889, 1508
FriOI GRGCYC 4 cut(s) 336, 414, 777, 1211
Fsp4HI GCNGC 8 cut(s) 167, 176, 179, 464, 467, 549, 881, 1080
FspBI CTAG 3 cut(s) 552, 981, 1244
GlaI GCGC 2 cut(s) 1031, 1379
GluI GCNGC 8 cut(s) 167, 176, 179, 464, 467, 549, 881, 1080
GsaI CCCAGC 3 cut(s) 642, 774, 1515
GsuI CTGGAG 2 cut(s) 248, 761
HapII CCGG 6 cut(s) 92, 450, 458, 545, 679, 1021
HgaI GACGC 1 cut(s) 92
HhaI GCGC 2 cut(s) 1032, 1380
Hin1I GRCGYC 2 cut(s) 84, 492
Hin1II CATG 8 cut(s) 809, 893, 989, 1079, 1271, 1406, 1412, 1586
Hin6I GCGC 2 cut(s) 1030, 1378
HinP1I GCGC 2 cut(s) 1030, 1378
HincII GTYRAC 2 cut(s) 34, 490
HindII GTYRAC 2 cut(s) 34, 490
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 6 cut(s) 8, 200, 425, 446, 1301, 1487
HpaII CCGG 6 cut(s) 92, 450, 458, 545, 679, 1021
HphI GGTGA 2 cut(s) 533, 1190
Hpy166II GTNNAC 7 cut(s) 34, 277, 490, 524, 575, 836, 915
Hpy188III TCNNGA 6 cut(s) 374, 499, 806, 1157, 1308, 1567
Hpy8I GTNNAC 7 cut(s) 34, 277, 490, 524, 575, 836, 915
HpyAV CCTTC 8 cut(s) 63, 211, 343, 731, 870, 1257, 1305, 1379
HpyCH4III ACNGT 2 cut(s) 1219, 1443
HpyCH4IV ACGT 3 cut(s) 480, 492, 712
HpyF10VI GCNNNNNNNGC 9 cut(s) 172, 175, 184, 409, 463, 1036, 1343, 1548, 1579
HpyF3I CTNAG 8 cut(s) 269, 387, 504, 540, 567, 782, 1171, 1618
HpySE526I ACGT 3 cut(s) 480, 492, 712
Hsp92I GRCGYC 2 cut(s) 84, 492
Hsp92II CATG 8 cut(s) 809, 893, 989, 1079, 1271, 1406, 1412, 1586
HspAI GCGC 2 cut(s) 1030, 1378
KroI GCCGGC 1 cut(s) 544
KroNI GCCGGC 1 cut(s) 546
Ksp22I TGATCA 1 cut(s) 288
Kzo9I GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
LmnI GCTCC 6 cut(s) 367, 460, 1036, 1421, 1508, 1627
Lsp1109I GCAGC 5 cut(s) 162, 190, 450, 892, 1066
LweI GCATC 2 cut(s) 811, 1623
MaeI CTAG 3 cut(s) 552, 981, 1244
MaeII ACGT 3 cut(s) 480, 492, 712
MaeIII GTNAC 4 cut(s) 201, 533, 694, 703
MalI GATC 8 cut(s) 267, 290, 323, 437, 556, 846, 1044, 1332
MbiI CCGCTC 2 cut(s) 1162, 1424
MboI GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
MboII GAAGA 9 cut(s) 131, 134, 146, 149, 412, 969, 1037, 1145, 1159
MflI RGATCY 3 cut(s) 554, 844, 1330
MhlI GDGCHC 6 cut(s) 279, 336, 414, 777, 1211, 1243
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 9 cut(s) 113, 589, 870, 937, 1279, 1294, 1463, 1532, 1574
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 3 cut(s) 2, 209, 1496
MmeI TCCRAC 5 cut(s) 224, 992, 1303, 1425, 1486
Mox20I TGGCCA 1 cut(s) 888
MroNI GCCGGC 1 cut(s) 544
MroXI GAANNNNTTC 2 cut(s) 1454, 1465
MscI TGGCCA 1 cut(s) 888
MseI TTAA 4 cut(s) 405, 585, 1230, 1385
MslI CAYNNNNRTG 4 cut(s) 359, 807, 990, 1407
Msp20I TGGCCA 1 cut(s) 888
MspA1I CMGCKG 2 cut(s) 175, 566
MspI CCGG 6 cut(s) 92, 450, 458, 545, 679, 1021
MspR9I CCNGG 6 cut(s) 92, 236, 679, 799, 1034, 1203
MvaI CCWGG 4 cut(s) 236, 799, 1034, 1203
MvnI CGCG 2 cut(s) 469, 1395
MwoI GCNNNNNNNGC 9 cut(s) 172, 175, 184, 409, 463, 1036, 1343, 1548, 1579
NaeI GCCGGC 1 cut(s) 546
NciI CCSGG 2 cut(s) 92, 679
NcoI CCATGG 1 cut(s) 889
NdeI CATATG 1 cut(s) 631
NdeII GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
NgoMIV GCCGGC 1 cut(s) 544
NlaIII CATG 8 cut(s) 809, 893, 989, 1079, 1271, 1406, 1412, 1586
NlaIV GGNNCC 4 cut(s) 99, 637, 1208, 1209
NmeAIII GCCGAG 2 cut(s) 355, 362
NmuCI GTSAC 2 cut(s) 201, 703
NspI RCATGY 2 cut(s) 1271, 1412
NspV TTCGAA 2 cut(s) 117, 1495
PaeR7I CTCGAG 3 cut(s) 335, 374, 957
PagI TCATGA 1 cut(s) 805
PceI AGGCCT 1 cut(s) 380
PciI ACATGT 1 cut(s) 1408
PcsI WCGNNNNNNNCGW 8 cut(s) 78, 114, 138, 150, 153, 420, 486, 495
PdiI GCCGGC 1 cut(s) 546
PdmI GAANNNNTTC 2 cut(s) 1454, 1465
PfeI GAWTC 3 cut(s) 425, 446, 1301
PflFI GACNNNGTC 1 cut(s) 494
PkrI GCNGC 8 cut(s) 168, 177, 180, 465, 468, 550, 882, 1081
PleI GAGTC 3 cut(s) 2, 208, 1495
PmaCI CACGTG 1 cut(s) 713
PmlI CACGTG 1 cut(s) 713
PpsI GAGTC 3 cut(s) 2, 208, 1495
Ppu21I YACGTR 1 cut(s) 713
PscI ACATGT 1 cut(s) 1408
PsiI TTATAA 1 cut(s) 756
Psp124BI GAGCTC 2 cut(s) 336, 414
Psp6I CCWGG 4 cut(s) 234, 797, 1032, 1201
PspCI CACGTG 1 cut(s) 713
PspFI CCCAGC 3 cut(s) 638, 770, 1511
PspGI CCWGG 4 cut(s) 234, 797, 1032, 1201
PspN4I GGNNCC 4 cut(s) 99, 637, 1208, 1209
PspOMI GGGCCC 1 cut(s) 1207
PspPI GGNCC 7 cut(s) 232, 238, 452, 635, 1207, 1208, 1247
PspXI VCTCGAGB 2 cut(s) 335, 957
PstI CTGCAG 1 cut(s) 180
PsuI RGATCY 3 cut(s) 554, 844, 1330
PsyI GACNNNGTC 1 cut(s) 494
PvuII CAGCTG 1 cut(s) 566
RsaI GTAC 2 cut(s) 835, 914
RsaNI GTAC 2 cut(s) 834, 913
RseI CAYNNNNRTG 4 cut(s) 359, 807, 990, 1407
SacI GAGCTC 2 cut(s) 336, 414
SalI GTCGAC 2 cut(s) 32, 488
SaqAI TTAA 4 cut(s) 405, 585, 1230, 1385
SatI GCNGC 8 cut(s) 167, 176, 179, 464, 467, 549, 881, 1080
Sau3AI GATC 8 cut(s) 265, 288, 321, 435, 554, 844, 1042, 1330
Sau96I GGNCC 7 cut(s) 232, 238, 452, 635, 1207, 1208, 1247
SchI GAGTC 3 cut(s) 2, 209, 1496
ScrFI CCNGG 6 cut(s) 92, 236, 679, 799, 1034, 1203
SduI GDGCHC 6 cut(s) 279, 336, 414, 777, 1211, 1243
SfaNI GCATC 2 cut(s) 811, 1623
SfcI CTRYAG 1 cut(s) 176
Sfr274I CTCGAG 3 cut(s) 335, 374, 957
SfuI TTCGAA 2 cut(s) 117, 1495
SinI GGWCC 2 cut(s) 238, 635
SlaI CTCGAG 3 cut(s) 335, 374, 957
SmiMI CAYNNNNRTG 4 cut(s) 359, 807, 990, 1407
SmlI CTYRAG 5 cut(s) 335, 374, 578, 957, 1544
SmoI CTYRAG 5 cut(s) 335, 374, 578, 957, 1544
Sse9I AATT 9 cut(s) 113, 589, 870, 937, 1279, 1294, 1463, 1532, 1574
SseBI AGGCCT 1 cut(s) 380
SspMI CTAG 3 cut(s) 552, 981, 1244
SstI GAGCTC 2 cut(s) 336, 414
StuI AGGCCT 1 cut(s) 380
StyD4I CCNGG 6 cut(s) 90, 234, 677, 797, 1032, 1201
StyI CCWWGG 4 cut(s) 309, 823, 889, 1118
TaaI ACNGT 2 cut(s) 1219, 1443
TaiI ACGT 3 cut(s) 483, 495, 715
TasI AATT 9 cut(s) 113, 589, 870, 937, 1279, 1294, 1463, 1532, 1574
TatI WGTACW 1 cut(s) 912
TauI GCSGC 3 cut(s) 169, 469, 551
TfiI GAWTC 3 cut(s) 425, 446, 1301
Tru1I TTAA 4 cut(s) 405, 585, 1230, 1385
Tru9I TTAA 4 cut(s) 405, 585, 1230, 1385
TscAI CASTG 2 cut(s) 210, 1224
TseFI GTSAC 2 cut(s) 201, 703
TseI GCWGC 5 cut(s) 175, 178, 463, 880, 1079
Tsp45I GTSAC 2 cut(s) 201, 703
TspDTI ATGAA 4 cut(s) 891, 930, 1350, 1391
TspGWI ACGGA 4 cut(s) 87, 230, 269, 418
TspRI CASTG 2 cut(s) 210, 1224
Tth111I GACNNNGTC 1 cut(s) 494
VneI GTGCAC 1 cut(s) 275
VpaK11BI GGWCC 2 cut(s) 238, 635
XagI CCTNNNNNAGG 2 cut(s) 1309, 1474
XapI RAATTY 5 cut(s) 113, 589, 937, 1294, 1463
XceI RCATGY 2 cut(s) 1271, 1412
XhoI CTCGAG 3 cut(s) 335, 374, 957
XmiI GTMKAC 2 cut(s) 33, 489
XmnI GAANNNNTTC 2 cut(s) 1454, 1465
XspI CTAG 3 cut(s) 552, 981, 1244
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.