Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr07

Orthogroup ID

OG0004085

Physical Location & Seq

Genomic Coordinates Reverse (-)

31911402 .. 31914179

Gene Structure 2778 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD07G1250500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1656 bp

ATGGCTGGTATACATTTCCTTGCCGGTTCCGCCCTGGGCGGTGCAGCTGCTAATGTCTGCATGCTAAGAATCGGTCGCATCTTGCTTGGTTATGGGGTTGGCTTTGCAAACCAGGTGGCTACCATTCTCTGCACCCTATTTTGTTTTATGGTTACATATTACGTAACACAGGTTTTAGGGTTGGAAGAAGCAATGGCAGTTGGGTTAGCAATAGCAGGCGAAGCCGGCCAATACAACGGCAGGATGACGCCCTTCGTCATCCTATCTTGCATGATGGCAGCCACAGGAGGTGTTATTTTCGGCTACGACATTGGAATTTCAGGCGGCGTGACATCAATGGAGCCGTTTCTGAAAAAGTTCTTCCCTGAGGTAAACACGAAGATGAAATCGGACACCAAAATCAGCAACTACTGTAAATTCGACAGCGAATTGTTGACCTCCTTCACATCCTCACTCTACATAGCTGGTCTTGTAGCTTCCCTTTTTGCCAGTTTGGTCACGAGAGCCTACGGCCGCAAGCCTTCAATCCTTGCCGGTGGAGCTGCGTTCCTTGCCGGCTCAGCTCTAAACGGTGCAGCTATGAACATCTACATGCTCATCCTCGGCCGTGTGTTGCTTGGAGTTGGTGTTGGTTTTGGAAATCAGGCTGTTCCTTTCGTCGGCATTGGCGCATTATCAGCTAACCTCATCAACTACGGCACTGAGAAGATCAAAGGCGGTTGGGGGTGGCGAATCTCCCTAGCCTTGGCCGCAGTCCCTGCCTCAGTGCTAACACTCGGCGCATTTTTCCTTCCGGAAACACCCAACAGCCTAATCCAGCGCAGCGCGGACCACCAAACAGCCAAGCTAATGCTGCAACGTATCAGAGGGGTCGATGATGTCCAAGTGGAACTCGATGATCTGATCAAAGCAAACAACATATCGAAAAACATGAAACACCCTTTCAAGAAAATCCTGGAGAGAAAGTATAGGCCTCAGCTTGTGATGGCAATAGCCATACCATTTTTTCAGCAAGTGACAGGGATCAATGTCATAGCCTTTTATGCCCCAATACTTTTTCGAACAATTGGGTTGGGAGAAAGCGCTTCGCTCTTGTCCTCCGTCATGACTGGCGTGGTTGGTACAATCTCAACGTTCATATCCATGCTTATCGTAGACAAATTCGGGCGAAGAGCATTGTTTTTAGCTGGGGGGATTCAAATGTTGGTGTCACAAATTATGGTGGGAGGTGTAATGGTAGCTCAGCTTGGTGATCACGGCGGAGTGAGCAAAGGGTATGCCTATTTGGTGCTGGTTTTGATATGCATTTACGTAGCAGGATTCGGGTGGTCGTGGGGGCCTCTCGGATGGTTGGTTCCGAGTGAGATTTTCCCATTGGAAATTCGATCGGCGGGACAAAGTATCAATGTGGTGGTGAACTTTTTGTTCACTTTCATTGTTGCTCAAACTTTTCTAGCCATGCTCTGCCACTTCAAGGCCGGGATTTTCTTCTTTTTCGGGGGTTGGGGGGTGGTGATGACGGTGTTTGTGTACTTGTTTTTGCCGGAGACGAAGAATGTGCCGATTGAGAAGATGGAGATAGTGTGGCAGGAGCATTGGTTTTGGAGGAGGGTTGTGGGGGATTTTAGCAAGGACACCAAGATGGAAGCTCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0016020

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Sugar_tr

Protein Analysis

552

Amino Acids

59.53

Weight (kDa)

9.22

Isoelectric Point (pI)

32.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Sugar_tr	PF00083	90 - 219	3.6e-24	Sugar (and other) transporter
Sugar_tr	PF00083	221 - 529	3e-81	Sugar (and other) transporter
MFS_1	PF07690	241 - 479	6.7e-10	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004085)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G61520 AT5G61520
fragaria_vesca	FvH4_5g33980 FvH4_7g27100 FvH4_7g27120
malus_domestica	MD01G1178300.v1.1 MD01G1180000.v1.1 MD07G1250500.v1.1
prunus_persica	Prupe.2G274100_v2.0.a1 Prupe.2G274200_v2.0.a1 Prupe.2G274300_v2.0.a1
pyrus_communis	pycom01g18910 pycom07g22350
rosa_chinensis	RchiOBHm_Chr1g0373911 RchiOBHm_Chr7g0233361
rosa_laevigata	RLG00000001275 RLG00000026796
rosa_multiflora	Rmu_sc0002022.1_g000003
rosa_roxburghii	Rroxscaffold_3G00227340 Rroxscaffold_4G00283910
rosa_rugosa	Rorug01G0379700 Rorug07G0280300 Rorug07G0280400
rosa_samantha	Rh1AG389900 Rh1BG354200 Rh1CG367300 Rh1DG384900 Rh7BG409000 Rh7CG455400
rosa_wichuraiana	Rw0G022460 Rw1G034530 Rw7G035880 Rw7G035920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	2 cut(s)	10, 1155
AccII	CGCG	1 cut(s)	827
AccIII	TCCGGA	1 cut(s)	793
AciI	CCGC	9 cut(s)	30, 39, 324, 514, 717, 750, 827, 1260, 1391
AclI	AACGTT	1 cut(s)	1133
AclWI	GGATC	1 cut(s)	1031
AcoI	YGGCCR	4 cut(s)	226, 511, 604, 747
AcsI	RAATTY	4 cut(s)	315, 416, 1160, 1380
AcyI	GRCGYC	1 cut(s)	248
AfaI	GTAC	2 cut(s)	1123, 1532
AfeI	AGCGCT	1 cut(s)	1084
AfiI	CCNNNNNNNGG	3 cut(s)	659, 745, 1474
AgsI	TTSAA	4 cut(s)	525, 946, 1199, 1474
AjnI	CCWGG	3 cut(s)	33, 111, 954
AloI	GAACNNNNNNTCC	2 cut(s)	1338, 1370
Alw26I	GTCTC	1 cut(s)	1541
AlwI	GGATC	1 cut(s)	1031
AlwNI	CAGNNNCTG	1 cut(s)	758
Aor13HI	TCCGGA	1 cut(s)	793
Aor51HI	AGCGCT	1 cut(s)	1084
AoxI	GGCC	7 cut(s)	226, 511, 604, 747, 971, 1337, 1476
ApeKI	GCWGC	7 cut(s)	44, 47, 278, 542, 575, 822, 853
ApoI	RAATTY	4 cut(s)	315, 416, 1160, 1380
Asp700I	GAANNNNTTC	1 cut(s)	356
AspLEI	GCGC	5 cut(s)	671, 782, 822, 827, 1085
AspS9I	GGNCC	2 cut(s)	829, 1337
AsuC2I	CCSGG	1 cut(s)	1480
AsuHPI	GGTGA	3 cut(s)	1262, 1426, 1525
AsuII	TTCGAA	1 cut(s)	1060
AvaII	GGWCC	1 cut(s)	829
AxyI	CCTNAGG	1 cut(s)	366
BauI	CACGAG	1 cut(s)	499
BbvCI	CCTCAGC	1 cut(s)	975
BbvI	GCAGC	7 cut(s)	34, 56, 290, 529, 587, 834, 840
BccI	CCATC	5 cut(s)	268, 979, 1341, 1567, 1636
BceAI	ACGGC	6 cut(s)	253, 328, 526, 591, 712, 1273
BcgI	CGANNNNNNTGC	2 cut(s)	1540, 1574
BciT130I	CCWGG	3 cut(s)	35, 113, 956
BclI	TGATCA	2 cut(s)	903, 1252
BcnI	CCSGG	1 cut(s)	1480
BcoDI	GTCTC	1 cut(s)	1541
BfaI	CTAG	2 cut(s)	740, 1454
BfoI	RGCGCY	1 cut(s)	1086
BlpI	GCTNAGC	2 cut(s)	559, 1242
Bme1390I	CCNGG	4 cut(s)	35, 113, 956, 1480
Bme18I	GGWCC	1 cut(s)	829
BmgT120I	GGNCC	2 cut(s)	829, 1337
BmiI	GGNNCC	4 cut(s)	28, 342, 1338, 1356
BmrFI	CCNGG	4 cut(s)	35, 113, 956, 1480
BmsI	GCATC	1 cut(s)	87
BpmI	CTGGAG	1 cut(s)	977
Bpu10I	CCTNAGC	1 cut(s)	975
Bpu1102I	GCTNAGC	2 cut(s)	559, 1242
Bpu14I	TTCGAA	1 cut(s)	1060
BpuMI	CCSGG	1 cut(s)	1480
BsaAI	YACGTR	2 cut(s)	163, 1312
BsaHI	GRCGYC	1 cut(s)	248
BsaJI	CCNNGG	4 cut(s)	33, 34, 601, 744
BsaWI	WCCGGW	1 cut(s)	793
BsaXI	ACNNNNNCTCC	8 cut(s)	612, 642, 950, 980, 1568, 1583, 1598, 1613
Bsc4I	CCNNNNNNNGG	3 cut(s)	659, 745, 1474
Bse118I	RCCGGY	4 cut(s)	23, 224, 533, 554
Bse1I	ACTGG	2 cut(s)	489, 1114
Bse21I	CCTNAGG	1 cut(s)	366
Bse3DI	GCAATG	1 cut(s)	198
BseAI	TCCGGA	1 cut(s)	793
BseBI	CCWGG	3 cut(s)	35, 113, 956
BseDI	CCNNGG	4 cut(s)	33, 34, 601, 744
BseGI	GGATG	5 cut(s)	249, 258, 446, 597, 1352
BseLI	CCNNNNNNNGG	3 cut(s)	659, 745, 1474
BseMI	GCAATG	1 cut(s)	198
BseMII	CTCAG	6 cut(s)	357, 573, 693, 777, 989, 1256
BseNI	ACTGG	2 cut(s)	489, 1114
BseRI	GAGGAG	1 cut(s)	1621
BseX3I	CGGCCG	2 cut(s)	511, 604
BseXI	GCAGC	7 cut(s)	34, 56, 290, 529, 587, 834, 840
BseYI	CCCAGC	1 cut(s)	1187
BsgI	GTGCAG	3 cut(s)	63, 115, 594
Bsh1236I	CGCG	1 cut(s)	827
Bsh1285I	CGRYCG	4 cut(s)	76, 514, 607, 1388
BshFI	GGCC	7 cut(s)	228, 513, 606, 749, 973, 1339, 1478
BsiEI	CGRYCG	4 cut(s)	76, 514, 607, 1388
BsiSI	CCGG	7 cut(s)	24, 225, 534, 555, 794, 1479, 1544
BslFI	GGGAC	2 cut(s)	740, 1407
BslI	CCNNNNNNNGG	3 cut(s)	659, 745, 1474
BsmAI	GTCTC	1 cut(s)	1541
BsmBI	CGTCTC	1 cut(s)	1541
BsmFI	GGGAC	2 cut(s)	740, 1407
BsnI	GGCC	7 cut(s)	228, 513, 606, 749, 973, 1339, 1478
Bsp119I	TTCGAA	1 cut(s)	1060
Bsp13I	TCCGGA	1 cut(s)	793
Bsp143I	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
Bsp1720I	GCTNAGC	2 cut(s)	559, 1242
BspACI	CCGC	9 cut(s)	30, 39, 324, 514, 717, 750, 827, 1260, 1391
BspANI	GGCC	7 cut(s)	228, 513, 606, 749, 973, 1339, 1478
BspCNI	CTCAG	6 cut(s)	358, 572, 694, 776, 988, 1255
BspEI	TCCGGA	1 cut(s)	793
BspFNI	CGCG	1 cut(s)	827
BspHI	TCATGA	1 cut(s)	1104
BspLI	GGNNCC	4 cut(s)	28, 342, 1338, 1356
BspPI	GGATC	1 cut(s)	1031
BspQI	GCTCTTC	1 cut(s)	1165
BspT104I	TTCGAA	1 cut(s)	1060
BsrDI	GCAATG	1 cut(s)	198
BsrFI	RCCGGY	4 cut(s)	23, 224, 533, 554
BsrI	ACTGG	2 cut(s)	489, 1114
BssAI	RCCGGY	4 cut(s)	23, 224, 533, 554
BssECI	CCNNGG	4 cut(s)	33, 34, 601, 744
BssMI	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
BssNAI	GTATAC	1 cut(s)	11
BssNI	GRCGYC	1 cut(s)	248
BssSI	CACGAG	1 cut(s)	499
BssT1I	CCWWGG	1 cut(s)	744
Bst1107I	GTATAC	1 cut(s)	11
Bst2BI	CACGAG	1 cut(s)	499
Bst2UI	CCWGG	3 cut(s)	35, 113, 956
Bst4CI	ACNGT	3 cut(s)	413, 572, 1522
Bst6I	CTCTTC	1 cut(s)	1165
BstACI	GRCGYC	1 cut(s)	248
BstAPI	GCANNNNNTGC	1 cut(s)	758
BstBAI	YACGTR	2 cut(s)	163, 1312
BstBI	TTCGAA	1 cut(s)	1060
BstC8I	GCNNGC	5 cut(s)	62, 217, 226, 518, 556
BstDEI	CTNAG	7 cut(s)	65, 366, 559, 702, 763, 975, 1242
BstF5I	GGATG	5 cut(s)	249, 258, 446, 597, 1352
BstFNI	CGCG	1 cut(s)	827
BstH2I	RGCGCY	1 cut(s)	1086
BstHHI	GCGC	5 cut(s)	671, 782, 822, 827, 1085
BstKTI	GATC	6 cut(s)	711, 901, 906, 1026, 1255, 1388
BstMAI	GTCTC	1 cut(s)	1541
BstMBI	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
BstMCI	CGRYCG	4 cut(s)	76, 514, 607, 1388
BstNI	CCWGG	3 cut(s)	35, 113, 956
BstNSI	RCATGY	2 cut(s)	64, 595
BstSCI	CCNGG	4 cut(s)	33, 111, 954, 1478
BstSNI	TACGTA	2 cut(s)	163, 1312
BstUI	CGCG	1 cut(s)	827
BstV1I	GCAGC	7 cut(s)	34, 56, 290, 529, 587, 834, 840
BstZ17I	GTATAC	1 cut(s)	11
BstZI	CGGCCG	2 cut(s)	511, 604
Bsu36I	CCTNAGG	1 cut(s)	366
BsuRI	GGCC	7 cut(s)	228, 513, 606, 749, 973, 1339, 1478
BtsCI	GGATG	5 cut(s)	249, 258, 446, 597, 1352
BtsIMutI	CAGTG	2 cut(s)	699, 771
Cac8I	GCNNGC	5 cut(s)	62, 217, 226, 518, 556
CaiI	CAGNNNCTG	1 cut(s)	758
CciI	TCATGA	1 cut(s)	1104
CfoI	GCGC	5 cut(s)	671, 782, 822, 827, 1085
Cfr10I	RCCGGY	4 cut(s)	23, 224, 533, 554
Cfr13I	GGNCC	2 cut(s)	829, 1337
CseI	GACGC	1 cut(s)	256
CsiI	ACCWGGT	1 cut(s)	111
Csp6I	GTAC	2 cut(s)	1122, 1531
CspCI	CAANNNNNGTGG	2 cut(s)	96, 131
CviAII	CATG	7 cut(s)	61, 271, 592, 931, 1105, 1144, 1459
CviQI	GTAC	2 cut(s)	1122, 1531
DdeI	CTNAG	7 cut(s)	65, 366, 559, 702, 763, 975, 1242
DpnI	GATC	6 cut(s)	710, 900, 905, 1025, 1254, 1387
DpnII	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
EaeI	YGGCCR	4 cut(s)	226, 511, 604, 747
EagI	CGGCCG	2 cut(s)	511, 604
Eam1104I	CTCTTC	1 cut(s)	1165
EarI	CTCTTC	1 cut(s)	1165
EciI	GGCGGA	2 cut(s)	19, 1275
EclXI	CGGCCG	2 cut(s)	511, 604
Eco105I	TACGTA	2 cut(s)	163, 1312
Eco130I	CCWWGG	1 cut(s)	744
Eco147I	AGGCCT	1 cut(s)	973
Eco47I	GGWCC	1 cut(s)	829
Eco47III	AGCGCT	1 cut(s)	1084
Eco52I	CGGCCG	2 cut(s)	511, 604
Eco81I	CCTNAGG	1 cut(s)	366
EcoO109I	RGGNCCY	1 cut(s)	1337
EcoRII	CCWGG	3 cut(s)	33, 111, 954
EcoT14I	CCWWGG	1 cut(s)	744
EcoT22I	ATGCAT	1 cut(s)	1307
ErhI	CCWWGG	1 cut(s)	744
Esp3I	CGTCTC	1 cut(s)	1541
FaeI	CATG	7 cut(s)	64, 274, 595, 934, 1108, 1147, 1462
FaqI	GGGAC	2 cut(s)	740, 1407
FatI	CATG	7 cut(s)	60, 270, 591, 930, 1104, 1143, 1458
FauI	CCCGC	1 cut(s)	1384
FbaI	TGATCA	2 cut(s)	903, 1252
FblI	GTMKAC	2 cut(s)	10, 1155
FokI	GGATG	5 cut(s)	245, 256, 433, 584, 1359
FspBI	CTAG	2 cut(s)	740, 1454
GlaI	GCGC	5 cut(s)	670, 781, 821, 826, 1084
GsaI	CCCAGC	1 cut(s)	1191
GsuI	CTGGAG	1 cut(s)	977
HaeII	RGCGCY	1 cut(s)	1086
HaeIII	GGCC	7 cut(s)	228, 513, 606, 749, 973, 1339, 1478
HapII	CCGG	7 cut(s)	24, 225, 534, 555, 794, 1479, 1544
HgaI	GACGC	1 cut(s)	256
HhaI	GCGC	5 cut(s)	671, 782, 822, 827, 1085
Hin1I	GRCGYC	1 cut(s)	248
Hin1II	CATG	7 cut(s)	64, 274, 595, 934, 1108, 1147, 1462
Hin6I	GCGC	5 cut(s)	669, 780, 820, 825, 1083
HinP1I	GCGC	5 cut(s)	669, 780, 820, 825, 1083
HincII	GTYRAC	1 cut(s)	435
HindII	GTYRAC	1 cut(s)	435
HinfI	GANTC	4 cut(s)	69, 732, 1195, 1320
HpaII	CCGG	7 cut(s)	24, 225, 534, 555, 794, 1479, 1544
HphI	GGTGA	3 cut(s)	1262, 1426, 1525
Hpy166II	GTNNAC	7 cut(s)	11, 373, 435, 1156, 1417, 1428, 1531
Hpy188I	TCNGA	6 cut(s)	351, 391, 866, 903, 1346, 1359
Hpy188III	TCNNGA	4 cut(s)	499, 794, 946, 1105
Hpy8I	GTNNAC	7 cut(s)	11, 373, 435, 1156, 1417, 1428, 1531
Hpy99I	CGWCG	1 cut(s)	662
HpyAV	CCTTC	4 cut(s)	262, 451, 531, 800
HpyCH4III	ACNGT	3 cut(s)	413, 572, 1522
HpyCH4IV	ACGT	4 cut(s)	162, 859, 1133, 1311
HpyCH4V	TGCA	8 cut(s)	44, 60, 107, 132, 270, 575, 856, 1305
HpyF3I	CTNAG	7 cut(s)	65, 366, 559, 702, 763, 975, 1242
HpySE526I	ACGT	4 cut(s)	162, 859, 1133, 1311
Hsp92I	GRCGYC	1 cut(s)	248
Hsp92II	CATG	7 cut(s)	64, 274, 595, 934, 1108, 1147, 1462
HspAI	GCGC	5 cut(s)	669, 780, 820, 825, 1083
Kpn2I	TCCGGA	1 cut(s)	793
KroI	GCCGGC	2 cut(s)	224, 554
KroNI	GCCGGC	2 cut(s)	226, 556
Ksp22I	TGATCA	2 cut(s)	903, 1252
Kzo9I	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
LguI	GCTCTTC	1 cut(s)	1165
LmnI	GCTCC	4 cut(s)	340, 539, 1591, 1654
Lsp1109I	GCAGC	7 cut(s)	34, 56, 290, 529, 587, 834, 840
LweI	GCATC	1 cut(s)	87
MabI	ACCWGGT	1 cut(s)	111
MaeI	CTAG	2 cut(s)	740, 1454
MaeII	ACGT	4 cut(s)	162, 859, 1133, 1311
MaeIII	GTNAC	6 cut(s)	151, 163, 328, 496, 1015, 1209
MalI	GATC	6 cut(s)	710, 900, 905, 1025, 1254, 1387
MboI	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
MboII	GAAGA	8 cut(s)	197, 352, 391, 718, 1182, 1480, 1564, 1582
MfeI	CAATTG	1 cut(s)	1065
MluCI	AATT	7 cut(s)	315, 416, 428, 1065, 1160, 1215, 1380
MmeI	TCCRAC	1 cut(s)	162
Mph1103I	ATGCAT	1 cut(s)	1307
MroI	TCCGGA	1 cut(s)	793
MroNI	GCCGGC	2 cut(s)	224, 554
MroXI	GAANNNNTTC	1 cut(s)	356
MslI	CAYNNNNRTG	4 cut(s)	380, 590, 1142, 1640
MspA1I	CMGCKG	1 cut(s)	47
MspI	CCGG	7 cut(s)	24, 225, 534, 555, 794, 1479, 1544
MspR9I	CCNGG	4 cut(s)	35, 113, 956, 1480
MteI	GCGCNGCGC	1 cut(s)	823
MunI	CAATTG	1 cut(s)	1065
MvaI	CCWGG	3 cut(s)	35, 113, 956
MvnI	CGCG	1 cut(s)	827
NaeI	GCCGGC	2 cut(s)	226, 556
NciI	CCSGG	1 cut(s)	1480
NdeII	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
NgoMIV	GCCGGC	2 cut(s)	224, 554
NlaIII	CATG	7 cut(s)	64, 274, 595, 934, 1108, 1147, 1462
NlaIV	GGNNCC	4 cut(s)	28, 342, 1338, 1356
NmeAIII	GCCGAG	2 cut(s)	582, 756
NmuCI	GTSAC	4 cut(s)	328, 496, 1015, 1209
NsiI	ATGCAT	1 cut(s)	1307
NspI	RCATGY	2 cut(s)	64, 595
NspV	TTCGAA	1 cut(s)	1060
PaeI	GCATGC	1 cut(s)	64
PagI	TCATGA	1 cut(s)	1104
PasI	CCCWGGG	1 cut(s)	34
PceI	AGGCCT	1 cut(s)	973
PciSI	GCTCTTC	1 cut(s)	1165
PdiI	GCCGGC	2 cut(s)	226, 556
PdmI	GAANNNNTTC	1 cut(s)	356
PfeI	GAWTC	4 cut(s)	69, 732, 1195, 1320
PfoI	TCCNGGA	1 cut(s)	954
Ple19I	CGATCG	1 cut(s)	1388
Ppu21I	YACGTR	2 cut(s)	163, 1312
Psp1406I	AACGTT	1 cut(s)	1133
Psp6I	CCWGG	3 cut(s)	33, 111, 954
PspFI	CCCAGC	1 cut(s)	1187
PspGI	CCWGG	3 cut(s)	33, 111, 954
PspN4I	GGNNCC	4 cut(s)	28, 342, 1338, 1356
PspPI	GGNCC	2 cut(s)	829, 1337
PstNI	CAGNNNCTG	1 cut(s)	758
PvuI	CGATCG	1 cut(s)	1388
PvuII	CAGCTG	1 cut(s)	47
RsaI	GTAC	2 cut(s)	1123, 1532
RsaNI	GTAC	2 cut(s)	1122, 1531
RseI	CAYNNNNRTG	4 cut(s)	380, 590, 1142, 1640
SapI	GCTCTTC	1 cut(s)	1165
Sau3AI	GATC	6 cut(s)	708, 898, 903, 1023, 1252, 1385
Sau96I	GGNCC	2 cut(s)	829, 1337
ScrFI	CCNGG	4 cut(s)	35, 113, 956, 1480
SexAI	ACCWGGT	1 cut(s)	111
SfaNI	GCATC	1 cut(s)	87
SfuI	TTCGAA	1 cut(s)	1060
SinI	GGWCC	1 cut(s)	829
SmiMI	CAYNNNNRTG	4 cut(s)	380, 590, 1142, 1640
SnaBI	TACGTA	2 cut(s)	163, 1312
SphI	GCATGC	1 cut(s)	64
Sse9I	AATT	7 cut(s)	315, 416, 428, 1065, 1160, 1215, 1380
SseBI	AGGCCT	1 cut(s)	973
SsiI	CCGC	9 cut(s)	30, 39, 324, 514, 717, 750, 827, 1260, 1391
SspMI	CTAG	2 cut(s)	740, 1454
StuI	AGGCCT	1 cut(s)	973
StyD4I	CCNGG	4 cut(s)	33, 111, 954, 1478
StyI	CCWWGG	1 cut(s)	744
TaaI	ACNGT	3 cut(s)	413, 572, 1522
TaiI	ACGT	4 cut(s)	165, 862, 1136, 1314
TaqI	TCGA	6 cut(s)	420, 873, 894, 923, 1060, 1384
TaqII	GACCGA	1 cut(s)	62
TasI	AATT	7 cut(s)	315, 416, 428, 1065, 1160, 1215, 1380
TatI	WGTACW	1 cut(s)	1530
TauI	GCSGC	3 cut(s)	327, 516, 752
TfiI	GAWTC	4 cut(s)	69, 732, 1195, 1320
TscAI	CASTG	2 cut(s)	706, 771
TseFI	GTSAC	4 cut(s)	328, 496, 1015, 1209
TseI	GCWGC	7 cut(s)	44, 47, 278, 542, 575, 822, 853
Tsp45I	GTSAC	4 cut(s)	328, 496, 1015, 1209
TspDTI	ATGAA	5 cut(s)	398, 596, 947, 1126, 1423
TspGWI	ACGGA	1 cut(s)	1090
TspRI	CASTG	2 cut(s)	706, 771
VpaK11BI	GGWCC	1 cut(s)	829
XapI	RAATTY	4 cut(s)	315, 416, 1160, 1380
XceI	RCATGY	2 cut(s)	64, 595
XmiI	GTMKAC	2 cut(s)	10, 1155
XmnI	GAANNNNTTC	1 cut(s)	356
XspI	CTAG	2 cut(s)	740, 1454
Zsp2I	ATGCAT	1 cut(s)	1307

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.